DE3567278D1 - Metachromatic dye sorption means for differential determination of sub-populations of lymphocytes - Google Patents

Metachromatic dye sorption means for differential determination of sub-populations of lymphocytes

Info

Publication number
DE3567278D1
DE3567278D1 DE8585902017T DE3567278T DE3567278D1 DE 3567278 D1 DE3567278 D1 DE 3567278D1 DE 8585902017 T DE8585902017 T DE 8585902017T DE 3567278 T DE3567278 T DE 3567278T DE 3567278 D1 DE3567278 D1 DE 3567278D1
Authority
DE
Germany
Prior art keywords
lymphocytes
populations
sub
differential determination
metachromatic dye
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DE8585902017T
Other languages
English (en)
Inventor
Lawrence Kass
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CYTOCOLOR INC., HINCKLEY, OHIO, US
Original Assignee
Lawrence Kass
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lawrence Kass filed Critical Lawrence Kass
Application granted granted Critical
Publication of DE3567278D1 publication Critical patent/DE3567278D1/de
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N2001/305—Fixative compositions
DE8585902017T 1984-04-27 1985-04-12 Metachromatic dye sorption means for differential determination of sub-populations of lymphocytes Expired DE3567278D1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US06/604,869 US4615878A (en) 1980-03-12 1984-04-27 Metachromatic dye sorption means for differential determination of sub-populations of lymphocytes
PCT/EP1985/000161 WO1985005179A1 (en) 1984-04-27 1985-04-12 Metachromatic dye sorption means for differential determination of sub-populations of lymphocytes

Publications (1)

Publication Number Publication Date
DE3567278D1 true DE3567278D1 (en) 1989-02-09

Family

ID=24421387

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8585902017T Expired DE3567278D1 (en) 1984-04-27 1985-04-12 Metachromatic dye sorption means for differential determination of sub-populations of lymphocytes

Country Status (4)

Country Link
US (1) US4615878A (de)
EP (1) EP0179107B1 (de)
DE (1) DE3567278D1 (de)
WO (1) WO1985005179A1 (de)

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Publication number Priority date Publication date Assignee Title
US4840784A (en) * 1982-12-22 1989-06-20 Eastman Kodak Company Use of pyrylium and thiapyrylium compounds as biological stains
US4810487A (en) * 1986-01-02 1989-03-07 Cytocolor, Inc. Method of identifying polymethine stained lymphocyte subpopulations and compositions thereof
JPH0668491B2 (ja) * 1986-06-30 1994-08-31 株式会社日立製作所 細胞自動分類装置
US5089384A (en) * 1988-11-04 1992-02-18 Amoco Corporation Method and apparatus for selective cell destruction using amplified immunofluorescence
US5340719A (en) * 1990-11-23 1994-08-23 Corporation Coulter Method and apparatus for optically screening microscopic cells
JPH06503171A (ja) * 1990-11-23 1994-04-07 クールター　コーポレイション 顕微鏡的細胞の光学的スクリーニング方法及び装置
US5348859A (en) * 1990-11-23 1994-09-20 Coulter Corporation Method and apparatus for obtaining an absolute white blood cell subset count and white blood cell multipart differential
US6136540A (en) 1994-10-03 2000-10-24 Ikonisys Inc. Automated fluorescence in situ hybridization detection of genetic abnormalities
US5352613A (en) * 1993-10-07 1994-10-04 Tafas Triantafillos P Cytological screening method
WO1996031237A2 (en) * 1995-04-04 1996-10-10 Wound Healing Of Oklahoma Cancer treatment by photodynamic therapy, in combination with an immunoadjuvant
US6316007B1 (en) * 1995-04-04 2001-11-13 Wound Healing Of Oklahoma Combined physical and immunotherapy for cancer
US6956695B2 (en) * 2001-03-19 2005-10-18 Ikonisys, Inc. System and method for increasing the contrast of an image produced by an epifluorescence microscope
WO2006085897A2 (en) 2004-05-13 2006-08-17 Advanced Animal Diagnostics Microfluidic device and leucocyte antigen mediated microfluidic assay
CN101553727A (zh) * 2006-08-04 2009-10-07 伊康尼西斯公司 用于显微系统的图象处理方法
WO2009117652A1 (en) 2008-03-21 2009-09-24 Abbott Point Of Care, Inc. Method and apparatus for determining the hematocrit of a blood sample utilizing the intrinsic pigmentation of hemoglobin contained within the red blood cells
US7929121B2 (en) * 2008-03-21 2011-04-19 Abbott Point Of Care, Inc. Method and apparatus for detecting and counting platelets individually and in aggregate clumps
WO2009117664A2 (en) 2008-03-21 2009-09-24 Abbott Point Of Care, Inc. Method and apparatus for determining red blood cell indices of a blood sample utilizing the intrinsic pigmentation of hemoglobin contained within the red blood cells
EP2260343B1 (de) * 2008-03-21 2016-09-21 Abbott Point Of Care, Inc. Verfahren und vorrichtung zur bestimmung einer fokuslage eines bildaufnahmegeräts zur darstellung einer biologischen probe
US8081303B2 (en) 2008-03-21 2011-12-20 Abbott Point Of Care, Inc. Method and apparatus for analyzing individual cells or particulates using fluorescent quenching and/or bleaching
CA2720299C (en) 2008-04-02 2015-02-17 Abbott Point Of Care, Inc. Self-calibrating gradient dilution in a constituent assay and gradient dilution apparatus performed in a thin film sample
EP2269033B1 (de) * 2008-04-09 2016-09-21 Abbott Point Of Care, Inc. Verfahren zur messund des bereichs einer probe in einer analysekammer
WO2009126505A1 (en) * 2008-04-09 2009-10-15 Abbott Point Of Care, Inc. Method of detecting very low levels of analyte within a thin film fluid sample contained in a thin thickness chamber
US20100255605A1 (en) * 2009-04-02 2010-10-07 Abbott Point Of Care, Inc. Method and device for transferring biologic fluid samples
ES2438841T3 (es) * 2009-12-31 2014-01-20 Abbott Point Of Care, Inc. Método y aparato para determinar el volumen celular medio de los glóbulos rojos en la sangre
US8472693B2 (en) 2010-03-18 2013-06-25 Abbott Point Of Care, Inc. Method for determining at least one hemoglobin related parameter of a whole blood sample
JP6463005B2 (ja) * 2013-05-30 2019-01-30 キヤノン株式会社 マクロファージ識別剤、該マクロファージ識別剤を用いた識別方法、分取方法、評価方法、スクリーニング方法、およびキット

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BE403179A (de) * 1933-05-16
US3617185A (en) * 1968-08-19 1971-11-02 Gaf Corp Stable highly concentrated solutions of basic dyes
US4146604A (en) * 1973-05-31 1979-03-27 Block Engineering, Inc. Differential counting of leukocytes and other cells
US4400370A (en) * 1980-03-12 1983-08-23 Lawrence Kass Metachromatic dye sorption means for differential determination of leukocytes
US4581223A (en) * 1980-03-12 1986-04-08 Lawrence Kass Individual leukocyte determination by means of differential metachromatic dye sorption
DE3014563A1 (de) * 1980-04-16 1981-10-29 Merck Patent Gmbh, 6100 Darmstadt Verfahren zur bestimmung leukaemischer zellen
US4500509A (en) * 1981-03-11 1985-02-19 Lawrence Kass Metachromatic dye sorption and fluorescent light emmisive means for differential determination of developmental stages of neutrophilic granulocytic cells and other leukocytes
US4492752A (en) * 1982-09-03 1985-01-08 Ortho Diagnostics Systems Inc. Method for discriminating between unstained and absorbing dye stained cells

Also Published As

Publication number Publication date
EP0179107A1 (de) 1986-04-30
EP0179107B1 (de) 1989-01-04
US4615878A (en) 1986-10-07
WO1985005179A1 (en) 1985-11-21

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Legal Events

Date Code Title Description
8327 Change in the person/name/address of the patent owner

Owner name: CYTOCOLOR INC., HINCKLEY, OHIO, US

8381 Inventor (new situation)

Free format text: KASS, LAWRENCE, HINCKLEY, OHIO, US

8364 No opposition during term of opposition