DE3326940A1 - ANALYSIS PROCEDURE - Google Patents

Description

PATENT ADVOCATE EUROPEAN PATENT ATTORNEY F. ENDUCH, POST BOX 13 20, D-S034 QERMERINO

TELEPHONE: "X") 84 30 38

TELEX: e21730pat «d CABLES: PATENDLY QERMERING FLOWER STREET β

D-8034 GERMERING July 25, 1983 E / m My files: D-5115

Applicant: Kabushiki Kaisha Daini Seikosha, Tokyo, Japan and Sanuki Kogyo Kabushiki Kaisha, Tokyo, Japan

Analysis procedure

The invention relates to an analysis method, in particular for carrying out a blood group determination can be used.

Lower when determining blood group or other determinations Parts of samples are usually glass plates with one or more flat recesses in which the reaction test is carried out will. The sample and the reagent are mixed in the recess. Known methods of this type are therefore carried out entirely manually and require a large amount of work and one accordingly long work hours.

It is therefore an object of the invention to provide an analysis method of the initially mentioned to improve said type in such a way that only a minimal amount of manual work is required.

This task is carried out by an automatic analysis method according to the Invention solved in which cylindrical reagent vessels with a small diameter are suspended on the circumference of a turntable so that they can be freely pivoted are pivotable in the radial direction. The sample and a reagent are then poured into the vessels and the turntable is then with rotated at high speed to produce a centrifugal separation after mixing and reaction to enable.

The invention is to be explained in more detail, for example, with the aid of the drawing. Show it:

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Fig. 1 is a schematic sectional view of a device for implementation

the method according to the invention;
Fig. 2 is a plan view of the turntable in Fig. 1; and FIG. 3 shows an enlarged sectional view through one of the reagent vessels in FIGS. 1 and 2.

The embodiment shown in Fig. 1 of a device for implementation of the method according to the invention comprises a turntable 2 which carries reagent vessels 1 along its circumference. The turntable 2 is by a motor 3 can be driven and its angle of rotation can be detected by a disk 4 to which a light barrier 5 or the like detection device is assigned is. The turntable can be stopped in predetermined positions and can also be rotated by the motor 3 at high speed.

Along the circumference of the turntable 2, support devices 6 are predetermined Spaced apart from each other. Each support device has two support pins 7 (Fig. 2 and 3), which are in a recess along the circumference of the turntable are arranged facing one another, pivotable about a support ring 8 to store, in each of which a reagent vessel 1 in the form of a small Test tubes can be arranged.

Two support plates 10a, 10b are independent of one another in the vertical direction arranged displaceably above the turntable 2 by drive devices 9a, 9b. A glass tube 11 provided with a drip nozzle for the sample and a number of glass tubes 12 for reagents are on the support disk 10a and 10b each arranged in a position which corresponds to the stopped position of the reagent vessels concerned .11. The glass tubes stand with a storage container 14 for the sample or with storage containers 15 for reagents Via connecting lines in connection, in each of which a pump 13 is provided. The drip nozzles of these glass tubes 11, 12 are in the out Fig. 3 angled manner as seen and in the case of the individual glass tubes in this way arranged differently so that none of the drip nozzles comes into contact with the same area of the inner wall of a reagent vessel 1, to a mutual To prevent contamination in the dropwise hydration. The ends of the glass tubes 11, 12 are in such a way as to face the inner wall the relevant reagent vessel 1 angled that when lowering the glass tube whose drip nozzle comes into contact with the relevant inner wall of the reagent vessel 1.

In using the device, the sample and reagents come first filled into the relevant storage container 14,15, and the empty reagent

vessels 1 are put in the turntable 2. Then the glass tube 11 lowered so that one or more droplets of the sample along the Inner wall of the reagent vessel 1 can be filled. If different determinations have to be carried out, the sample is supplied in successive rotary positions of the turntable.

Then the glass tubes 12 are lowered to accept a reagent drop by drop to fill along the inner wall of the reagent vessel 1 concerned. In the Storage containers 15 can contain different reagents, so that they can be used in different rotational positions of the turntable 2 for the respective reagent vessel can be fed. If desired, the sample can be filled manually into a reagent vessel 1, which is then inserted into the turntable 2 will.

After the sample and reagents are added dropwise as described the turntable 2 rotated at high speed so that the contents of the reagent vessels flows through the occurring centrifugal forces to the bottom and is mixed there.

The supply of the sample and the reagents by the pumps and the rotation of the turntable are sequentially performed by an automatic control mechanism that includes a microprocessor with a predetermined program contains. Such support structures can also be used that the reagent vessels are always arranged at an incline to the turntable.

Therefore, in the method described, the reagent vessels are made in this way arranged on a turntable that can be rotated at high speed, that they can be pivoted in the radial direction and the sample and the reagents can be reliably mixed in the relevant vessels. The method can therefore be carried out automatically, so that in particular when examining many different samples, for example in the case of hematological serial examinations, considerable labor savings are achieved can be.

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Claims (2)

• β O θ β β F. FINALLY, POST BOX 13 26. D-8034 GERMERINQ .-PHYS. FINALLY PATENT ADVOCATE EUROPEAN PATENT ATTORNEY TELEPHONE: (089) 64 35 3Θ TELEXr 52 1730 rate d CABLES: PATENOLICH G BLOSSOM OF FLOWERS 8 D-8QS4 GERMERSf «. 25. Ju! I 1983 E / m My file: D-5115 Applicant: Kabushiki Kajsha Daini Seikosha, Tokyo, Japan and Sanuki Kogyo Kabushiki Kaisha, Tokyo, Japan Claims ( Ly analysis method which can be used in particular to carry out blood group determinations, characterized in that cylindrical reagent vessels with a small diameter are used along the circumference of a turntable in such a way that they can be freely pivoted in the radial direction of the turntable, so that the sample and reagents in the reagent vessels along them Then the turntable is rotated at high speed in order to collect and mix the sample and reagents in the bottom of the vessels. 2. Device for performing the method according to claim 1, characterized support devices (6) arranged by a turntable (2) / enx! ang its circumference for an arrangement which can be pivoted in the radial direction of reagent vessels (1), by a detection device (4,5) for the Rotary position of the turntable, which can be rotated between predetermined rotational positions by a drive device (3), by one in the vertical direction adjustable device for lowering glass tubes (11, 12) for feeding the sample or the reagents at different points along the inner wall of the reagent vessels, and by a programmed control circuit to drive the turntable at high speed, so that the supplied Liquids at the bottom of the reagent vessels .gesammelt and thoroughly mixed will.