CN2390207Y - Microfluid biological chip analysis plate - Google Patents
Microfluid biological chip analysis plate Download PDFInfo
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- CN2390207Y CN2390207Y CN 99229740 CN99229740U CN2390207Y CN 2390207 Y CN2390207 Y CN 2390207Y CN 99229740 CN99229740 CN 99229740 CN 99229740 U CN99229740 U CN 99229740U CN 2390207 Y CN2390207 Y CN 2390207Y
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Abstract
The utility model relates to a detection and analysis plate of a microfluid biochip and a detection method thereof, which applies the microfluid biochip to chemical or biologic analysis systems. The detection and analysis plate of a microfluid biochip is composed of a chip plate and a cover plate, wherein, the chip plate is provided with micro flute passages which are connected with reaction sites; the transparent cover plate seals the chip plate, or the chip plate is provided with the reaction sites, and the cover plate is provided with micro flutes. The detection method is that probes or marked object molecules of different types are respectively fixed on the reaction sites of the detection and analysis plate of a microfluid biochip, and each ingredient of the same test sample on the same chip is simultaneously analyzed.
Description
The utility model microfluid biochip detection-analysis board relates to is a kind of microfluid biochip to be applied to the check and analysis plate of chemistry or bioanalysis and reaction system, especially adopts microfluidic channel to connect the new design of many reflecting points.In very short time, can analyze the required result who obtains multiple composition simultaneously for same raw material or determinand.
Biochip mainly is meant by Micrometer-Nanometer Processing Technology and supermolecule self-assembling technique, in microanalysis unit and the system that the solid chip surface makes up.Biochip can integrate many difference in functionality devices, for example, the pre-service of biological sample, the extraction of inhereditary material, the amplification of specific gene fragment, biological probe array and Capillary Electrophoresis form whole microfluid system, with realize to compound, protein, nucleic acid, cell and other biological components accurately, fast, the screening or the detection of large information capacity.Genetic chip is a most important class biochip, and it the is integrated gene probe of dense arrangement in a large number can be analyzed a large amount of genes at short notice, makes people can promptly read and analyze the program of life.
Biochip has extremely important meaning on biological detection, medical test and medical diagnosis on disease, drug screening and gene sequencing.For example in biology, along with molecular biological continuous development, since the Human Genome Project particularly highly visible was implemented, the data of related nucleic acid, protein sequence and structure were exponential increase.And the most challenging work of next century is exactly after the Human Genome Project is finished, and promptly at the back era gene, how we use a large amount of biomolecule information services in human society, and makes medical diagnosis and treatment produce fundamental change.In medical science, " the subordinate phase medical science on system, organ, tissue, the cell level " transforms to " on the gene level; DNA → RNA → protein → protein and nucleic acid interaction, and the phase III medical science on they and the environmental interaction level ".This gene diagnosis of carrying out on molecular level and gene therapy will be familiar with the root that disease produces at all, and will be hopeful basic understanding and treat the major disease that comprises cancer.The fundamental change of these biology, medical science, a mensuration and the analysis that basic prerequisite is a gene order.Can carry out gene sequencing and analysis effectively apace, will have influence on the enforcement of the Human Genome Project, thereby influence further developing of biology, medical science.The methods that biomolecule detection adopted such as tradition gene comprise a series of numerous and diverse steps such as chemical reaction, gel electrophoresis, these method spended times are longer, and operate complicated, especially time-consuming and be not suitable for portability and check order fast aspect large scale sequencing.Traditional gene tester is being carried out in the improved process, is that the emerging technology of representative is arisen at the historic moment with the biochip.Biochip technology is with many discontinuous analytic process related in the life science, and as specimen preparation, chemical reaction and analyzing and testing etc. are by adopting microelectronics, technologies such as micromechanics are integrated in the chip, make it serialization, integrated, microminiaturization and robotization.The strong means that provide are obtained and analyzed to the maturation of this technology and use and will bring a revolution in medical diagnosis on disease and the life science association areas such as treatment, new drug development, judicial expertise, food and environment of next century for biological information.
96 orifice plates are existing a kind of conventional utensils that are used for the medical science biochemistry detection that generally use.96 reaction tanks on it are isolated from each other and do not pollute mutually, can select the number of reaction tank arbitrarily for use according to user's needs.But required amount of reagent is still bigger, also is not easy to continued operation simultaneously, more is not easy to simultaneously to multi-component continuous fast detecting in the sample.
The purpose of this utility model is the weak point that exists at conventional utensil in the present clinical medicine routine inspection, a kind of microfluid biochip detection-analysis board is proposed, can carry out the continuous express-analysis of many index based on this check and analysis plate, and sample and reagent consumption are little, the operation simple and feasible.
Microfluid biochip detection-analysis board takes following scheme to realize:
Its structure of microfluid biochip detection-analysis board is made of chip board, cover plate, be furnished with little gutter channel on the chip board, the passage first line of a couplet respond site a, b, c, d, transparent cover plate covers the sealing chip board, or on chip board, be furnished with reaction site, transparent cover plate is provided with little groove, cover plate is to have little groove one cover on chip board, little groove on the cover plate joins the reaction site on the chip board, forms a sealing microfluid biochip detection-analysis board with inflow and outflow port.
The microfluid biochip detection-analysis board that the utility model proposes can carry out multi-component continuous express-analysis to micro sample, detects simplely, and the application of microfluid biochip detection-analysis board in check and analysis is following realization:
The probe molecule or the marker molecules of multiple biological or chemical are placed respectively and are fixed on little reaction site of microfluid biochip detection-analysis board, each little reaction site is only fixed a kind of, every kind of probe molecule or marker molecules only with determinand in a certain component hybridize, crosslinked or specific chemical reaction takes place.The sample of detected analysis and various analytical reagent inject according to the program of setting and flow into port, the required little reaction site of flowing through, and produce corresponding effect.Above-mentioned specific reaction, hybridization or crosslinked will not be disturbed in the absorption of other materials.Even interference is arranged, also can be got rid of by methods such as washings.Little reaction site also can take following method to obtain: promptly can constitute by in advance probe substance or label being fixed on the minute solid phase carrier, putting on the site that is provided with on the analysis plates again.Since the probe molecule of each little reaction site or marker molecules only with determinand in specific component react, do not disturb mutually again each other, just can carry out qualitative or quantitative test to each component in the same sample simultaneously on chip piece, the gained result can detect with optics, electrical method.For example available microscope, CCD observation or surperficial excimer resonance (SPR), surface interfere reflection optics, electric field technology such as (RIFS) to observe and provide the result, also available other mark or cold method detection.Thereby increase work efficiency greatly, reduce reagent dosage, really accomplish quick, accurate, robotization and pollution-free.Probe molecule is meant and can produces the interactional material with single-minded recognition material function of specificity with specifying chemistry and biological substance, can adopt biomacromolecule material (as protein, nucleic acid, DNA, PNA, cell etc.), chemicals and polymer substance probe, label can adopt biological substance, chemical substance.
The microfluid biochip detection-analysis board that the utility model proposes, little reaction of being carried out on each little reflecting point can be quickened and regulates and control by physical methods such as electricity, light, sound, for example, can on each little reflecting point, microelectrode be set respectively, control little reaction on the reaction site by the regulation and control electric field.By the size and Orientation of Control current, the required reactant of characteristic reaction is assembled fast, unwanted material leaves fast, thereby adds fast response, improves detection sensitivity and shortens detection time.
The utility model is described in further detail below with reference to accompanying drawing.
Fig. 1 is one of microfluid biochip detection-analysis board that the utility model proposes.
Fig. 2 is the cover plate corresponding with microfluid biochip detection-analysis board shown in Figure 1 that the utility model proposes.
Fig. 3 is two of the microfluid biochip detection-analysis board that the utility model proposes.
Fig. 4 is the cover plate corresponding with microfluid biochip detection-analysis board shown in Figure 3 that the utility model proposes.
Fig. 5 is three of the microfluid biochip detection-analysis board that the utility model proposes.
Fig. 6 is the cover plate corresponding with Fig. 5 microfluid biochip detection-analysis board that the utility model proposes.
Fig. 7 is four of the microfluid biochip detection-analysis board that the utility model proposes.
Fig. 8 is the cover plate corresponding with Fig. 7 microfluid biochip detection-analysis board that the utility model proposes.
Fig. 9 is five used diaphragms of the microfluid biochip detection-analysis board that the utility model proposes.
Figure 10 is five a chip analysis plate of the microfluid biochip detection-analysis board that the utility model proposes.
Figure 11 is the cover plate of the microfluid biochip detection-analysis board corresponding with Fig. 9, Figure 10 that the utility model proposes.
Figure 12 is six of the microfluid biochip detection-analysis board that the utility model proposes.
Figure 13 is the cover plate corresponding with microfluid biochip detection-analysis board shown in Figure 12 that the utility model proposes.
Constitute by chip board, cover plate with reference to accompanying drawing microfluid biochip detection-analysis board structure, be furnished with little gutter channel on the chip board, the passage first line of a couplet respond site a, b, c, d, transparent cover plate covers the sealing chip board, or on chip board, be furnished with reaction site, transparent cover plate is provided with little groove, cover plate is to have little groove one cover on chip board, little groove on the cover plate joins the reaction site on the chip board, forms a sealing microfluid biochip detection-analysis board with inflow and outflow port.
With reference to accompanying drawing 1 is a kind of tandem type plastics (or metal and other material) microfluid biochip detection-analysis boards (abbreviation micro fluidic plate), on this fluid plate, be furnished with little gutter channel with inflow and outflow port, the passage first line of a couplet respond site a, b, c, d ..., these reaction site can be micro reaction pool, reactive tank or other forms.
With reference to accompanying drawing 2 is the chip transparent cover plate (glass or plastics) corresponding with Fig. 1, can cover sealing A plate, makes it to form the micro fluidic plate of sealing.
With reference to accompanying drawing 3,4 is another kind of chip design method, and characteristics are to be furnished with uniform little reaction site on chip board shown in Figure 3.A, b, c, d ..., this slightly reaction site can be regular shapes such as small sircle hole, strip groove, also can be other form.
Fig. 4 is the transparent cover plate corresponding with Fig. 3 chip board, and this cover plate is provided with little groove, when cover plate when having little groove one cover on the A plate, the little groove on the cover plate just in time joins the reflecting point on the chip board.Make the reaction site on the chip board connect to the analysis plates that an envelope increases with microfluid inflow and outflow port by little gutter channel.
With reference to accompanying drawing 5,6, be another kind of chip analysis plate design.On the chip board of Fig. 5, be furnished with a plurality of little grooves, each little groove several little reaction site of connecting.Fig. 6 is the cover plate with the corresponding chip board of Fig. 5.With reference to accompanying drawing 7,8, be another kind of chip board design.On chip analysis plate shown in Figure 7, be furnished with several rows reaction site independent of each other, several reaction site independent of each other arranged with delegation.Fig. 8 is and the corresponding cover plate of Fig. 7 chip analysis plate.Cover and be furnished with some independently little grooves.When cover plate covered on the chip board shown in Figure 7 with the one side that little groove is arranged, each little groove just in time got up delegation's reaction site series connection.
Fig. 9,10,11 is the design of another kind of microfluid biochip detection-analysis board.But Fig. 9 is the diaphragm of fixing biological molecules such as common nylon, fixes some kinds of different molecular probes and biological substance in the above with conventional method.Figure 10 is distributed with microwell array and is associated with the micropore transparent cover plate of pipeline, and Figure 11 is a base plate, diaphragm is clipped between transparent micropore cover plate and the base plate, and the biological fixation site on the diaphragm is overlapped with micropore on the transparent micropore cover plate.Make integral body become a micro fluidic plate.Can the specific reaction on the diaphragm be detected by the optics aspect equally.
Figure 12,13 is the design of another kind of microfluid biochip detection-analysis board.On the chip board of Figure 12, be furnished with the parallel little reflecting point of some row, whenever the little reflecting point that lists does not link to each other each other mutually, Figure 13 is the cover plate corresponding with Figure 12 chip board, on the cover board be furnished with the parallel minitype channel of several rows, 1,2 ... n, be drilled with some micropores between each passage and the cover plate face, microchannel and cover plate face are coupled together.This one side that is drilled with micropore at cover plate is furnished with the little groove of bent type some parallel to each other arranged side by side with above-mentioned microchannel capwise in addition, this slightly groove do not communicate with microchannel and micropore.When this that will have micropore and little groove earlier simultaneously covers on the chip board shown in Figure 12, each row micropore is just in time corresponding identical with little reflecting point of respectively being listed as on the same position of little reflecting point on the chip board, for example, the first row micropore is respectively with respectively to be listed as first little reflecting point of little reflecting point corresponding identical overlapping on the chip board on the cover plate, if the direction of cover plate along curved little groove moved a certain distance, micropore on the cover plate no longer with chip board on little reflecting point overlapping, then with the curved little groove on the cover plate each the little reflecting point on the chip board is coupled together, each curved little groove connects row.If continue to move a certain distance along same direction again, little groove on the cover plate and the little reflecting point on micropore and the chip board will be not overlapping.During use, the little groove on can cover plate links up the little reaction site that respectively is listed as on the chip board, by inlet 1,2 ... n feeds reactant, and these reactants can be identical, also can be different.The translation cover plate is with the micropore on the cover plate, on chip board then.Little reaction site is overlapping, again by the microchannel a on the cover plate
1, a
2, a
3A
n, feed second kind of reactant, these reactants can be identical, also can be different, Using such method, also can continue to introduce the third, the 4th kind ... reactant.If need, a kind of reactant of every adding can make little groove or the little reaction site on micropore and the chip board on the cover plate not overlapping by mobile again cover plate, and each little reaction site is isolated each other fully, reacts certain hour under the condition of setting.Above-mentioned this design can be avoided cross pollution mutually between each little reaction site.After reaction finishes, can cover plate on little groove each little reaction site on the chip board is linked up, feed washing reagent or distilled water washs by inlet.The end product of reaction can detect and provide the result with the method for optics or electricity.
Claims (6)
1. microfluid biochip detection-analysis board, it is characterized in that: its structure of analysis plates is made of chip board, cover plate, be furnished with little gutter channel on the chip board, the passage first line of a couplet site that responds, transparent cover plate covers the sealing chip board, or is furnished with reaction site on chip board, and transparent cover plate is provided with little groove, cover plate is to have little groove one cover on chip board, and the little grooved surface on the cover plate joins the reaction site on the chip board.
2. microfluid biochip detection-analysis board according to claim 1 is characterized in that: (a) preparation has a series of little reaction site on same chip board; (b) this slightly reaction site have by what little gutter channel connected to a sealing that microfluid flows into and the analysis plates of outflow port.
3. microfluid biochip detection-analysis board according to claim 1 is characterized in that reaction site can be micro reaction pool, reactive tank or other forms.
4. microfluid biochip detection-analysis board according to claim 1, but it is characterized in that between chip board and cover plate clamping has the diaphragm of fixing biological molecules.
5. according to the described microfluid biochip detection-analysis board of claim 1, it is characterized in that multiple probe substance that is used to detect and biological substance can be in advance according to the array and the sizes that design, be fixed on nylon membrane, on the fiber diaphragms such as acetate fibre paper, be placed on then between chip board and the cover plate, probe on the fiber diaphragm or the reaction site on biological substance dot matrix and chip board or the cover plate are coincided, make it to constitute a miniflow system.
6. according to the described microfluid biochip detection-analysis board of claim 1, it is characterized in that each little reaction site can be furnished with microelectrode, control little reaction on the reaction site by the regulation and control electric field.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99229740 CN2390207Y (en) | 1999-10-27 | 1999-10-27 | Microfluid biological chip analysis plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99229740 CN2390207Y (en) | 1999-10-27 | 1999-10-27 | Microfluid biological chip analysis plate |
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| Publication Number | Publication Date |
|---|---|
| CN2390207Y true CN2390207Y (en) | 2000-08-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 99229740 Expired - Fee Related CN2390207Y (en) | 1999-10-27 | 1999-10-27 | Microfluid biological chip analysis plate |
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| CN (1) | CN2390207Y (en) |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7645423B2 (en) | 2004-08-20 | 2010-01-12 | International Business Machines Corporation | Optical micro plugs for multichannel and multilayer pharmaceutical device |
| US7744817B2 (en) | 2003-08-11 | 2010-06-29 | Sakura Finetek U.S.A., Inc. | Manifold assembly |
| US7767152B2 (en) | 2003-08-11 | 2010-08-03 | Sakura Finetek U.S.A., Inc. | Reagent container and slide reaction retaining tray, and method of operation |
| US8459509B2 (en) | 2006-05-25 | 2013-06-11 | Sakura Finetek U.S.A., Inc. | Fluid dispensing apparatus |
| CN103255052A (en) * | 2013-05-08 | 2013-08-21 | 中山大学达安基因股份有限公司 | Electrochemistry gene chip |
| US8580568B2 (en) | 2011-09-21 | 2013-11-12 | Sakura Finetek U.S.A., Inc. | Traceability for automated staining system |
| CN103808771A (en) * | 2012-11-15 | 2014-05-21 | 赵朝辉 | Biochemical reaction basin for electrochemical biochip |
| US8752732B2 (en) | 2011-02-01 | 2014-06-17 | Sakura Finetek U.S.A., Inc. | Fluid dispensing system |
| US8932543B2 (en) | 2011-09-21 | 2015-01-13 | Sakura Finetek U.S.A., Inc. | Automated staining system and reaction chamber |
| CN104502516A (en) * | 2015-01-27 | 2015-04-08 | 天津出入境检验检疫局工业产品安全技术中心 | Micro-fluidic refractive index detection method used for polymerized triglyceride |
| US9518899B2 (en) | 2003-08-11 | 2016-12-13 | Sakura Finetek U.S.A., Inc. | Automated reagent dispensing system and method of operation |
-
1999
- 1999-10-27 CN CN 99229740 patent/CN2390207Y/en not_active Expired - Fee Related
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9518899B2 (en) | 2003-08-11 | 2016-12-13 | Sakura Finetek U.S.A., Inc. | Automated reagent dispensing system and method of operation |
| US7744817B2 (en) | 2003-08-11 | 2010-06-29 | Sakura Finetek U.S.A., Inc. | Manifold assembly |
| US7767152B2 (en) | 2003-08-11 | 2010-08-03 | Sakura Finetek U.S.A., Inc. | Reagent container and slide reaction retaining tray, and method of operation |
| US7645423B2 (en) | 2004-08-20 | 2010-01-12 | International Business Machines Corporation | Optical micro plugs for multichannel and multilayer pharmaceutical device |
| US8459509B2 (en) | 2006-05-25 | 2013-06-11 | Sakura Finetek U.S.A., Inc. | Fluid dispensing apparatus |
| US9914124B2 (en) | 2006-05-25 | 2018-03-13 | Sakura Finetek U.S.A., Inc. | Fluid dispensing apparatus |
| US9016526B2 (en) | 2011-02-01 | 2015-04-28 | Sakura Finetek U.S.A., Inc. | Fluid dispensing system |
| US8752732B2 (en) | 2011-02-01 | 2014-06-17 | Sakura Finetek U.S.A., Inc. | Fluid dispensing system |
| US8932543B2 (en) | 2011-09-21 | 2015-01-13 | Sakura Finetek U.S.A., Inc. | Automated staining system and reaction chamber |
| US10295444B2 (en) | 2011-09-21 | 2019-05-21 | Sakura Finetek U.S.A., Inc. | Automated staining system and reaction chamber |
| US9005980B2 (en) | 2011-09-21 | 2015-04-14 | Sakura Finetek U.S.A., Inc. | Traceability for automated staining system |
| US8580568B2 (en) | 2011-09-21 | 2013-11-12 | Sakura Finetek U.S.A., Inc. | Traceability for automated staining system |
| CN103808771A (en) * | 2012-11-15 | 2014-05-21 | 赵朝辉 | Biochemical reaction basin for electrochemical biochip |
| CN103255052A (en) * | 2013-05-08 | 2013-08-21 | 中山大学达安基因股份有限公司 | Electrochemistry gene chip |
| CN103255052B (en) * | 2013-05-08 | 2014-07-30 | 中山大学达安基因股份有限公司 | Electrochemistry gene chip |
| CN104502516A (en) * | 2015-01-27 | 2015-04-08 | 天津出入境检验检疫局工业产品安全技术中心 | Micro-fluidic refractive index detection method used for polymerized triglyceride |
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| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |