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Publication numberCN1955711 A
Publication typeApplication
Application numberCN 200610161067
Publication date2 May 2007
Filing date19 Dec 2003
Priority date20 Dec 2002
Also published asCA2507960A1, CA2507960C, CA2508000A1, CA2508001A1, CA2508070A1, CA2508113A1, CA2508323A1, CA2508323C, CA2508370A1, CA2511032A1, CA2974221A1, CN1726275A, CN1726386A, CN100424166C, CN100472197C, EP1572979A1, EP1572979A4, EP1573297A1, EP1573311A2, EP1573311A4, EP1573312A2, EP1573312A4, EP1573408A2, EP1573408A4, EP1576376A2, EP1576376A4, EP1579190A1, EP1579190A4, EP1579194A2, EP1579194A4, US7400983, US7648678, US7758809, US7937228, US7960178, US8216512, US8257968, US8298815, US8386195, US8394635, US8529836, US8663978, US8673642, US8784735, US8788217, US8969086, US9040284, US9229016, US9599630, US9778273, US20040266015, US20050064535, US20060045806, US20060046298, US20060063265, US20060085140, US20060088928, US20060088940, US20060172426, US20080241876, US20100081167, US20110167930, US20110269238, US20120310422, US20130029409, US20130084567, US20130203103, US20130217108, US20130330252, US20140038232, US20140186218, US20140234170, US20140286838, US20140356935, US20160084862, WO2004057307A1, WO2004058404A2, WO2004058404A3, WO2004058950A1, WO2004059284A2, WO2004059284A3, WO2004059287A2, WO2004059287A3, WO2004059288A2, WO2004059288A3, WO2004059297A1, WO2004059441A2, WO2004059441A3
Publication number200610161067.9, CN 1955711 A, CN 1955711A, CN 200610161067, CN-A-1955711, CN1955711 A, CN1955711A, CN200610161067, CN200610161067.9
Inventors道格斯威特, 马克基, 戈登法因格尔德, 克里斯托弗布坎南, 鲍勃莱思罗普, 约翰法武齐
Applicant丹麦达科有限公司
Export CitationBiBTeX, EndNote, RefMan
External Links: SIPO, Espacenet
Method and apparatus for automatic staining of tissue samples
CN 1955711 A
Abstract  translated from Chinese
本发明涉及一种组织样品染色装置(1)。 The present invention relates to a tissue sample dyeing device (1). 所述装置(1)包括用于接收至少一个试剂容器(3)和至少一个样品的工作区,和用于将至少一种试剂施加在至少一个样品上的探头。 The device (1) comprises means for receiving at least one reagent container (3) and at least one sample of the work area, and at least one reagent is applied for at least one sample probe. 其特征在于具有光学传感器,其构造成记录和自动染色装置中的至少一个部件相关的图象数据。 Characterized by having an optical sensor configured to record automatic staining apparatus and at least one component associated with the image data. 本发明还涉及一种在自动染色装置(1)中染色样品的方法。 The present invention also relates to an automatic staining device (1) sample staining method.
Claims(21)  translated from Chinese
1.一种自动染色装置,包括:工作区,用于接收至少一个试剂容器(3)和至少一个样品;和探头,用于将至少一种试剂施加在至少一个样品上;其特征在于:光学传感器,其构造成记录和自动染色装置中的至少一个部件相关的图象数据。 An automatic staining apparatus, comprising: a working area for receiving the at least one reagent container (3) and at least one sample; and a probe for applying at least one reagent on at least one sample; characterized in that: the optical a sensor configured to record automatic staining apparatus and at least one component associated with the image data.
2.根据权利要求1所述的装置,其特征在于:所述至少一个部件包括从由以下部分构成的组合中选择的要素:样品载体、载玻片(7)、试剂容器(3)、自动染色装置(1)中的位置、自动染色装置(1)中的元件、二维高分辨率符号码、数据矩阵码、条形码、粘附标记、二维符号区和人可读的文本区。 2. The apparatus according to claim 1, wherein: said element comprises at least one member selected from the group consisting of the following parts: sample carrier, slides (7), reagent container (3), automatically dyeing device (1) position, the automatic staining device (1) of the element, a high-resolution two-dimensional symbol number, data matrix codes, bar codes, adhesion markers, two-dimensional symbol regions and human-readable text area.
3.根据权利要求1或2所述的装置,其特征在于:所述至少一个部件是一个生物样品。 3. The apparatus according to claim 1 or claim 2, wherein: said at least one member is a biological sample.
4.根据权利要求1所述的装置,其特征在于:所述生物样品是一个染色的生物样品。 4. The apparatus according to claim 1, wherein: said biological sample is a biological sample stained.
5.根据权利要求1所述的装置,其特征在于:该装置还包括一个机器人部件(20),该机器人部件适合在所述的工作区内活动并能够容纳所述探头。 5. The apparatus according to claim 1, characterized in that: the apparatus further comprises a robot member (20), the robot means adapted in the work area and to accommodate the activities of the probe.
6.根据权利要求1所述的装置,其特征在于:该装置还包括控制部件,所述机器人部件响应该控制部件。 6. The apparatus according to claim 1, characterized in that: the apparatus further comprises a control means, responsive to the control means of the robot means.
7.根据权利要求1所述的装置,其特征在于:所述光学传感器(86)是一个图象捕获的二维光学传感器(86)。 7. The apparatus according to claim 1, wherein: said optical sensor (86) is a two-dimensional image captured by the optical sensor (86).
8.根据权利要求1所述的装置,其特征在于:所述光学传感器(86)产生一个被记录图象数据的数字表示形式。 8. The apparatus according to claim 1, wherein: said optical sensor (86) generates an image data to be recorded in the digital representation.
9.根据权利要求8所述的装置,其特征在于:还包括一个装置,用于将所述数字表示形式发送到一个远端位置以进行远程分析或者远程存贮。 9. The apparatus according to claim 8, characterized in that: further comprising a means for sending said digital representation to a remote location for remote analysis or remote storage.
10.根据权利要求1所述的装置,其特征在于:还包括一个存贮装置,用于存贮被记录的图象数据。 10. The apparatus according to claim 1, characterized in that: further comprising a storage means for storing image data to be recorded.
11.一种在自动染色装置(1)中染色样品的方法,包括以下步骤:在一个工作区内提供至少一个样品;在所述工作区内提供至少一个试剂容器;提供一个用于将至少一种试剂施加在至少一个样品上的探头;和提供一个光学传感器用来记录和自动染色装置中的至少一个部件相关的图象数据。 An automatic staining apparatus (1) in a sample staining method, comprising the steps of: providing at least one of a workspace sample; providing at least one reagent container in the work area; providing a means for at least one and at least one member to provide an optical sensor for recording and automatic staining apparatus associated image data; reagents applied to the sample at least one probe.
12.根据权利要求11所述的方法,其特征在于:所述至少一个部件包括从由以下部分构成的组合中选择的要素:样品载体、载玻片(7)、试剂容器(3)、自动染色装置(1)中的位置、自动染色装置(1)中的元件、二维高分辨率符号码、数据矩阵码、条形码、粘附标记、二维符号区和人可读的文本区。 12. The method according to claim 11, wherein: said element comprises at least one member selected from the group consisting of the following parts: sample carrier, slides (7), reagent container (3), automatically dyeing device (1) position, the automatic staining device (1) of the element, a high-resolution two-dimensional symbol number, data matrix codes, bar codes, adhesion markers, two-dimensional symbol regions and human-readable text area.
13.根据权利要求11所述的方法,其特征在于:所述至少一个部件是一个生物样品。 13. The method according to claim 11, wherein: said at least one member is a biological sample.
14.根据权利要求13所述的方法,其特征在于:所述生物样品是一个染色的生物样品。 14. The method according to claim 13, wherein: said biological sample is a biological sample stained.
15.根据权利要求11所述的方法,其特征在于:还包括以下步骤:提供一个机器人部件(20),该机器人部件适合在所述的工作区内活动并能够容纳所述探头(10)。 15. The method according to claim 11, characterized in that: further comprising the steps of: providing a robot member (20), the robot means adapted in said working area and capable of accommodating said active probe (10).
16.根据权利要求15所述的方法,其特征在于:还包括以下步骤:提供一个控制部件,所述机器人部件响应该控制部件。 16. The method according to claim 15, characterized in that: further comprising the steps of: providing a control means, responsive to the control means of the robot means.
17.根据权利要求11所述的方法,其特征在于:所述光学传感器(86)是一个可捕获图象的二维光学传感器(86)。 17. The method according to claim 11, wherein: said optical sensor (86) is a two-dimensional optical sensor to capture an image (86).
18.根据权利要求11所述的方法,其特征在于:还包括以下步骤:存贮被记录的图象数据。 18. The method according to claim 11, characterized in that: further comprising the steps of: storing image data to be recorded.
19.根据权利要求11所述的方法,其特征在于:还包括以下步骤:产生一个被记录图象数据的数字表示形式。 19. The method according to claim 11, characterized in that: further comprising the steps of: generating a digital image data to be recorded representation.
20.根据权利要求19所述的方法,其特征在于:还包括以下步骤:发送所述数字表示形式到一个远端位置。 20. The method according to claim 19, characterized in that: further comprising the step of: transmitting said digital representation to a remote location.
21.根据权利要求20所述的方法,其特征在于:还包括以下步骤:对所述数字表示形式进行即时远程检查或者远程存档。 21. The method according to claim 20, characterized in that: further comprising the step of: said digital representation of the instant remote inspection or remote archive.
Description  translated from Chinese
组织样品自动染色方法和装置 Tissue staining method and apparatus for automated sample

本案是中国申请号为200380106521.5、申请日为2003年12月19日的题为“组织样品自动染色方法和装置”的分案申请。 This case was Chinese Patent Application No. 200380106521.5, filed as "tissue sample automatic dyeing method and device" divisional application entitled December 19, 2003 of.

技术领域 Technical Field

本发明涉及一种组织样品自动染色的装置和方法。 The present invention relates to an apparatus and method for automatically staining tissue samples. 本发明进一步涉及用于样品处理以及样品处理的数据收集、数据维护和数据检索的系统。 The present invention further relates to methods for sample preparation and sample handling of data collection, data maintenance and data retrieval systems. 本发明特别涉及的应用领域包括免疫组织化学、原位杂交、荧光原位杂交、特异性染色和细胞学,以及潜在的其它化学和生物学应用。 The present invention particularly relates to the application include chemical, in situ hybridization, fluorescence in situ hybridization, specific staining and cytology, as well as other potential chemical and biological applications of immunohistochemistry.

背景技术 Background

免疫组织化学(IHC)应用和其它化学和生物学分析(例如原位杂交、特异性染色和细胞学)中的组织样品处理,要求一个或多个处理次序或规程作为一个或多个样品分析的一部分。 Immunohistochemistry (IHC) applications and other chemical and biological analysis (for example, in situ hybridization, specific staining and cytology) in the tissue sample, require one or more processing order or regulation as one or more sample analysis part. 这些样品处理次序或规程是由要求分析的个体或组织(例如医院的病理学家和组织学家)规定的,或者由待实施的特定分析指令(例如由组织规定的标准方案)来规定。 These samples were processed by order or regulation requires an individual or organization to analyze (such as a hospital pathologist and histologist) regulations, or to be implemented by a specific analysis instructions (such as a standard protocol defined by the organization) to specify.

在样品分析的制备中,生物样品可以通过公知的样品获取技术来取得,并且可以包括在一些应用中甚至是一个或多个分离的细胞的组织。 In the preparation of the samples analyzed, the biological sample can be obtained by a known technique to obtain samples and may be included in some applications even one or more cells of the tissue separation. 组织样品可承载在样品载体(例如载玻片、或者也许是显微镜载玻片)上。 Tissue samples can be carried in the sample carrier (such as a slide, or perhaps a microscope slide) on.

例如,免疫应用需要包括诸如脱蜡、靶回收和染色这些步骤的处理次序或规程。 For example, applications need to include such immunity dewaxing, the processing order or the statutes target recovery and staining of these steps. 以前,在一些应用中,这些步骤由人工来实施,因此潜在地导致这是一个耗时的规程并且需要人员主动参与到样品处理中。 Previously, in some applications, to implement these steps by hand, thus potentially leading to it is a time consuming procedure and requires people to actively participate in the sample processing. 尤其是关于染色过程,多种组织载玻片的自动染色设备是公知的,因为为了满足对有利样品处理以及几乎不用人工烦琐操作的需求,已经对样品处理的自动化作了一些尝试。 Particularly with regard to the dyeing process, many organizations slides automatic dyeing machines are known, because in order to meet the demand for favorable treatment and almost no manual sample tedious operation, automated sample handling has made some attempts.

本发明的各个方面尤其可应用于具有一个或多个处理步骤的样品处理中,所述处理步骤是在一个样品、一部分或全部样品上进行的,这些规程在一些情况下是由呈现样品的各个载体来识别的。 In particular, various aspects of the present invention may be applied to one or more sample processing steps, the processing step is a sample, a part or all of the samples, in some cases these procedures are presented by the respective sample to identify the carrier. 本发明的各个方面尤其可应用于免疫组织化学(IHC)技术、以及原位杂交(ISH)和荧光原位杂交(FISH),特别是包括样品染色的技术。 Various aspects of the invention are particularly applicable to immunohistochemistry (IHC) techniques, and in situ hybridization (ISH) and fluorescence in situ hybridization (FISH), especially including sample staining technique.

本发明的实施例进一步涉及样品处理的自动控制系统。 Embodiments of the present invention further relates to a sample of the automatic control system. 这些实施例还涉及样品处理的数据收集、数据维护、数据检索,特别是处理规程和处理状态的信息分享,例如各个样品或多批处理、样品诊断特性、以及多批处理的实时或适应能力。 These embodiments also relate data sample collection, data maintenance, data retrieval, in particular, the processing procedures and processing status information to share, for example, or each sample batch, a sample diagnostic features, as well as multi-batch or real-time adaptability.

US5,839,091公开了一种组织自动染色的装置,其中将显微镜载玻片排列成许多行,而将试剂瓶储存在毗邻此载玻片部分的部分中。 US5,839,091 discloses an automatic tissue staining apparatus wherein the microscope slide arranged in a plurality of rows, and the reagent bottles stored in the adjoining section of this slide glass section. 机器人头按照控制程序从瓶中拾取预定量的试剂,将这些量的试剂沉积在预定的载玻片上,并吹散载玻片上的液体。 Robot control program in accordance with the pickup head from a predetermined amount of reagent bottle, the amount of these reagents is deposited on a predetermined slides and disperse the liquid on the slide. 此程序在与染色装置连接的计算机上运行。 This program runs on a computer connected to the dyeing apparatus. 染色装置装载有许多载玻片,每个载玻片及其位置都登记在计算机中,并选择染色次序。 Dyeing device loaded with a lot of slides, each slide and its location are registered in the computer, and select dyed order. 该程序还接收有关试剂及其在试剂部分上的位置的数据。 The program also receives data about the agent and its location in the reagent portion. 基于这些载玻片和试剂位置数据,该程序计算染色运行并控制机器人在染色装置中的运动。 Based on these slides and reagents location data, the program calculates the staining run and control the robot motion in the dyeing apparatus.

US6,352,861公开了一种转盘型自动染色装置,其中将载玻片布置在可旋转的转盘式载玻片支持体上,将试剂同样布置在载玻片支持体上方的可旋转的转盘式试剂支持体上。 US6,352,861 discloses a rotary-type automatic staining apparatus in which the slides are arranged on a rotatable carousel slides support, the reagent is also arranged above the slides support a rotatable reagent carousel Support body. 然后将特定载玻片旋转到输送区,将特定的试剂瓶也旋转到该位置,分配试剂到载玻片上。 The slides were then rotated to convey a specific area, a specific reagent bottles are rotated to the position assigned agents to the slide. 载玻片和试剂瓶配有条形码,且提供相关的条形码读取器来分别识别载玻片和试剂。 Slides and reagent bottle with a bar code, and provide a bar code reader to separately identify slides and reagents. 吹散区和识别区也配备在载玻片转盘的四周。 Disperse zone and the identification zone is also equipped with slides around the turntable. 载玻片条形码识别载玻片样品及其特定样品所需的特定免疫组织化学过程。 Slides slides sample bar code recognition and specific sample required for a specific immunohistochemical procedure. 定位试剂条形码读取器,以扫描试剂瓶上的试剂条形码。 Location reagent barcode reader to scan the reagent bottle on the reagent barcode. 来自载玻片条形码读取器和试剂条形码读取器的扫描信息被馈送到计算机内,并分别与载玻片和试剂转盘的索引位置相关。 Scan information from the slides and reagent barcode reader barcode reader is fed into the computer, and are associated with the index position of the slides and reagent carousel. 此信息用来旋转载玻片转盘和试剂转盘,以便为每个载玻片的每个载玻片处理步骤将正确的试剂瓶定位在分配区中。 This information is used to rotate the turntable slides and reagent carousel to the correct reagent bottle positioned in the distribution area for each slide processing steps of each slide.

'091中描述的自动染色装置的缺陷是,载玻片部分和试剂部分的每个组织载玻片和每个试剂瓶的位置,必须分别人工输入到计算机中,因为控制程序不能检查特定载玻片和试剂瓶的部位。 'Defect automatic staining apparatus 091 described is part of the position slides and reagents part of each organization and slides of each bottle must be manually entered into the computer, respectively, because the control program can not check for a specific slide glass portion of the wafer and the reagent bottle. 这样就带来一些风险:用错误的染色规程处理错位的载玻片,并且使染色装置在使用中非常不灵活。 This brings some risks: the wrong procedure dyeing process misplaced slides, and the staining device in use is very inflexible.

虽然'861专利采用了载玻片和试剂的条形码识别,但是这种转盘型装置在运行染色规程时是耗时的,因为这涉及到对于规程中的每个步骤,要用全部试剂存货旋转转盘,并用所有的载玻片旋转载玻片转盘。 Although the '861 patent uses a bar code identification slides and reagents, but the dial-type device when you run the dyeing procedure is time consuming, as it relates to each step in the procedure, to use all reagents inventory rotating turntable and rotating the turntable slides with all the slides. 这些旋转是耗时的,并使得这种类型的装置不适合运行大量的载玻片。 These rotations are time-consuming, and makes this type of device is not suitable for running a large number of slides. 而且,条形码仅能携带少量数据,一般是简单的识别码,这意味着控制计算机必须配有有关识别码的相应数据。 Also, the bar code can only carry a small amount of data, typically a simple identification code, which means that the control computer must be equipped with the appropriate data relating to the identification code.

发明的公开内容本发明的一个目的是,提供一种开始提到的那种组织样品的自动处理装置,这种装置能够自动识别机器中存在的试剂和载玻片存货。 It is an object of the present invention, the disclosure of the invention is to provide an automatic processing apparatus of a kind of tissue samples mentioned at the beginning, that means the machine can automatically identify the presence of reagents and slides later. 另一个目的是,提供对装置相关特性的识别,以便在新装载的载玻片的染色过程启动之前,进行自动制备检查。 Another object is to provide identification of the device-related features for slides staining procedure before starting a new load for automatic preparation of examination.

在一个实施例中,这些目的是通过开始提到的那种装置实现的,其中机器人部件(也许具有机器人头)配有光学传感器(或者也许是2-D光学传感器装置),所述传感器用于检查相关特性的二维图象数据并具有将所捕获的图象数据馈送到控制装置的能力。 In one embodiment, the object is achieved by apparatus of the kind mentioned at the beginning to achieve, where the robot member (perhaps having a robot head) is equipped with an optical sensor (2-D or perhaps the optical sensor device), the sensor is used Check correlation characteristic two-dimensional image data and captured with the image data supplied to the control device capacity.

本发明还提供了一种识别自动染色装置中的至少一个特性的方法,所述染色装置也许包括至少一个载玻片阵列和试剂阵列,以及也用试剂实施载玻片染色的机器人部件(或者也许是机器人装置);所述方法在一个实施例中包括以下步骤:在机器人装置的机器人头上配备光学传感器装置,将所述机器人头上的光学传感器装置移动到预定位置,记录所述位置的相关图象数据,以及将所述图象数据馈送到控制系统,以便根据所述图象数据操纵染色过程。 The present invention also provides an automatic staining apparatus identifying at least one characteristic of the method, the dyeing apparatus may include at least one array of slides and reagents arrays, as well as the implementation of the robot means also slides stained with the reagent (or perhaps the robot apparatus); In one embodiment, the method includes the steps of: a robot head robot apparatus provided with an optical sensor means, means for moving the optical sensor of the robot head to a predetermined position, the position of the recording related image data, and to feed the image data to the control system, in order to manipulate the image data in accordance with the dyeing process.

而且,本发明涉及一种在自动染色装置中染色组织样品的方法,所述染色装置也许包括至少一个载玻片阵列和试剂阵列,以及按照组织样品的特异性染色规程也用试剂实施载玻片染色的机器人部件(或者也许是机器人装置);所述方法在一个实施例中包括以下步骤:在机器人装置的机器人头上配备光学传感器装置,将所述机器人头上的光学传感器装置移动到预定位置,用所述光学传感器装置记录所述位置的相关图象数据; Further, the present invention relates to a method for the automatic staining apparatus for staining of tissue samples, the staining apparatus may include at least one array of slides and reagents arrays, and tissue samples according to protocol-specific staining reagent embodiment also slides Robot member dyed (or perhaps robot apparatus); In one embodiment the method comprises the steps of embodiment: the head of the robot in the robot apparatus is provided with an optical sensor device, the sensor means for moving said optical head to a predetermined position of the robot , with the recording of the location of the optical sensor means associated image data;

将所述图象数据馈送到控制系统,以便根据所述图象数据操纵染色过程;以及也利用来自试剂容器的试剂给组织样品染色。 The image data is fed to the control system, in order to manipulate the dyeing process according to the image data; and also the use of a reagent to stain the tissue sample from the reagent container.

在实施例中,自动染色装置1(是用至少一些自动操作染色的任何装置)可包括试剂容器3。 In an embodiment, the automatic staining apparatus 1 (with at least some of any automated staining apparatus) may comprise a reagent container 3. 一个或多个试剂容器3可位于自动染色装置1的试剂区域2。 One or more reagent containers 3 may be located in a reagent zone 2 of the automatic staining apparatus. 自动染色装置还可包括在此处可位于载玻片上的组织样品74。 Automatic staining apparatus may further include a tissue sample can be located here on the slide 74. 其上具有组织样品的多个载玻片也可位于自动染色装置1的载玻片区域5。 Having a plurality of slides on which tissue sample slide glass may also be located in the region 5 of the automatic staining apparatus. 本发明在实施例中还可包括机器人部件20、一些类型的控制部件、甚至光学传感器(86),也许是图象捕获2-D光学传感器。 In an embodiment of the present invention may also include a robot part 20, some types of control means, even an optical sensor (86), may be 2-D image capture optical sensor. 正如所容易理解的,控制部件85可以是计算机、软件程序、或者仅仅是特定的可编程的处理器功能。 As can be readily appreciated, the control member 85 may be a computer, a software program, or just a particular programmable processor functions.

如上所述,本发明能够光学探测二维图象。 As described above, the present invention is capable of detecting an optical two-dimensional image. 这可通过图像捕获2-D传感器来实现,所述传感器在自动染色装置1中可提供部件的二维图象。 This can be captured by the image sensor to achieve the 2-D, the sensor in an automatic staining apparatus can provide two-dimensional image member. 通过提供具有2-D光学传感器或装置的机器人部件(也许是机器人头),正如在一个实施例中,公共图象处理装置能够具有多个功能。 By providing the robot member having a 2-D or optical sensor means (perhaps robot head), as in one embodiment, the common image processing apparatus capable of having a plurality of functions. 通过利用2-D光学图象处理系统,染色装置的控制系统可容易地适合读取多种数据表示形式,由此部件或者是装置部分的实际图象一样,从而能够评估装置的状况。 By using 2-D optical image processing system, the control system of the dyeing device can easily be adapted to read a plurality of data representation, whereby a member or part of the actual image of the same apparatus, it is possible to assess the status of the device. 光学传感器或光学传感器装置可用来自动识别装置中存在的载玻片和试剂容器,由此光学传感器或光学传感器装置可用来检查载玻片是否在某一载玻片位置错位或缺失等。 An optical sensor or an optical sensor device may be used to automatically identify slides and reagent present in the container means, whereby the optical sensor or an optical sensor device may be used to check whether the slides in a slide glass misplaced or missing location.

光学传感器使按照本发明的染色装置具有迄今不可预见的灵活性并且能够使染色装置中的识别功能自动化。 The optical sensor having flexibility hitherto unforeseen dyeing device according to the present invention and the dyeing apparatus enables automatic identification function. 通过也许在机器人头、甚至是机器人部件上采用CCD-照相机等,可以不再需要用于每个识别任务的各个识别装置。 Perhaps in the head by a robot, and even use CCD- camera on the robot components, etc., you can no longer required for the individual identification means for each identified task. 这意味着,对装置的控制和维修容易进行。 This means that, for the control and maintenance of the device easier. 控制染色装置的软件适合包括装置的多种特性和状况(包括载玻片和试剂信息)的自动识别。 Dyeing device control software for automatic identification includes a variety of features and condition of the device (including the slides and reagent information). 通过利用识别染色装置中相关信息的方法和实施按照本发明的染色过程的方法,自动染色过程耗时更少且包括更多的定性检查,而在载玻片的染色操作中不会怎么降低速度。 Dyeing apparatus by using identification information about the methods and embodiments in accordance with the method of the present invention, the dyeing process, dyeing process automatically takes less and include more qualitative examination, the slide and the dyeing operation is not how slow .

在本发明的一个实施例中,试剂区域容纳多个试剂容器,这些容器固定布置成多行。 In one embodiment of the present invention, the reagent zone containing a plurality of reagent containers, which are arranged a plurality of rows is fixed. 同样,组织样品容纳在载玻片上,这些载玻片在染色过程中在至少一个染色区域或在玻片部分固定布置成多行。 Similarly, tissue samples on glass slides to accommodate, at least one of these slides stained area in the dyeing process or in the slide portion arranged in a plurality of fixed lines. 这些区域的布局使得其代表机器人头的基本上平坦的平台工作区,此工作区可以在X-轴和Y-轴上移动。 Layout such that these areas representative of the head of the robot platform substantially planar workspace, the workspace is movable in the X- and Y- axes. 在一个特别优选的实施例中,一行载玻片和/或试剂被移去或更换,而不会干扰染色过程。 In a particularly preferred embodiment, the line slides and / or reagent is removed or replaced without disturbing the dyeing process.

在另一个优选实施例中,染色装置包括至少两个被试剂区域分开的染色区域,即它们布置成使至少一些组织样品靠近至少一些试剂容器。 In another preferred embodiment, the staining apparatus comprising at least two separate reagent area stained area, i.e. they are arranged so that at least some of the tissue sample is close to at least some of the reagent container. 借此,可以显著限制机器人头为了到达所有载玻片而需要的运动,并借此增大染色装置的容量,由此可减少运行染色规程的时间或得到其它优点。 In this way, it can significantly limit the movement of the robot head in order to reach all the slides and needs, and to increase the capacity of dyeing apparatus, thereby reducing the running time of staining procedures or obtain other advantages. 进一步意识到,利用这种载玻片和试剂部分的布局还可达到更短的处理时间或其它优点,而无需可视系统例如光学传感器。 Further appreciated that use of this reagent arrangement section slides and also achieve shorter processing time or other advantages, such as an optical system without visual sensor.

在本发明的其它实施例中,光学传感器可以是照相机,或者也许包括CCD部件。 In other embodiments of the present invention, the optical sensor may be a camera, or a CCD may include member. 通过词汇“照相机”,应该理解,可以采用任何图象捕获装置,而无论是薄膜、板、存储器,还是任何类型的电子介质,而无论其成象的是光、可视电磁辐射,或者甚至是诸如现在公知的不可视的电磁辐射。 By the term "camera" should be understood, any image capture device, and whether it is a film, sheet, memory, or any type of electronic media, regardless of imaging light, visible electromagnetic radiation, or even Now known as the non-visible electromagnetic radiation. 通过记录相关图象、相关图象数据、甚至记录数字图象数据,利用公知的已经可用的图象处理能力,可以快速的方式在控制系统中完成该数据的计算机处理。 By recording the relevant image, associated image data, and even recording a digital image data using well-known image processing capabilities already available, can be quickly accomplished computer processing of the data in the control system. 而且,通过利用该数字技术,能够用高分辨率记录相对复杂的图象,由此在机器人头连续穿过载玻片标记时,可实现对几个识别(例如一整行载玻片上的标记)的快速记录,于是可避免每个载玻片识别的终止时间和开始时间。 Moreover, by using the digital technology to high-resolution images with relatively complex record, thereby continuously through the slides when the robot head mark, enabling several identification (such as a full line mark on the slide) Fast record, so to avoid identification of each slide end time and start time. 然而,通过本发明,意识到,根据图象解析的需要,还可采用其它图象传感器例如固态传感器,或者也许是CMOS传感器。 However, by the present invention, it realized according to the image resolution required, other image sensors may be employed such as solid state sensors, or perhaps CMOS sensor.

如上所述,光学传感器适合记录装置中存在的各个试剂容器或试剂瓶以及载玻片。 As described above, the optical sensor means for recording the presence of individual reagent containers or reagent bottle, and slides. 虽然其可给更大的区域、甚至整个设备成像,但是它可以构造成以电子、光学或定位的方式单独进行成象。 While it may be to a larger area, or even the entire imaging device, but it can be configured to electronic, optical or targeted manner imaging alone. 无论如何,作为成象能力的结果,无需装载到自动染色装置内的载玻片或试剂容器或试剂瓶的预定位置,因为载玻片和试剂瓶一旦装载到装置内,装置就适合自动识别新的载玻片和试剂瓶。 In any event, as a result of imaging capabilities, without slides or loaded into a predetermined position of the reagent bottle or reagent container automatic staining apparatus, because once the slides and reagent bottle loaded into the apparatus, the apparatus is adapted to automatically recognize the new slides and reagent bottles.

在一个实施例中,试剂容器和载玻片配有光学识别部件。 In one embodiment, the reagent container and slides with optical recognition component. 例如,试剂容器可配有试剂光学识别部件,载玻片可配有载玻片光学识别部件。 For example, the reagent container can be equipped with optical recognition component reagents, slides can be equipped with optical recognition component slides. 这些光学识别部件可包含有关试剂种类的机器可读的数据以及有关瓶内试剂的其它相关数据,载玻片识别部件可含有有关组织样品的数据,例如病人的识别、染色规程等。 Other relevant data which may contain information about the optical component recognition reagent type machine-readable data and relevant reagent bottle, slides may contain data identifying component related tissue sample, such as identification, staining and other patient procedures. 光学识别部件可包括重申信息、甚至冗余信息。 Optical recognition means may include reiterated information, even redundant information. 这可包括重复、甚至部分重复的信息,并且甚至包括可以是或者可以不是有关类似信息的不同译本的信息。 This may include repeated, and even some duplicate information, and even different versions may or may not information about a similar message.

光学识别部件或者替换型光学识别装置可以在或安装在试剂容器上或在载玻片上,这样光学识别部件可以被光学传感器读取。 Alternatively optical components or optical identification means may identify or mounted on the reagent container or on a slide, so that the optical member can be read by an optical recognition sensor. 通过位于“之上(on)”,预计任何相关的方式都能够包括;由此应该理解,无需单独的固定或表面安装。 By situated "above (on)", is expected in any way relevant can include; should thus be appreciated that no separate fixed or surface mounting. 同样,通过在“上方(above)”,应该理解,这不仅存在于诸如有关重力的定义中,而且还存在于诸如大致垂直地在表面等上方的象形定义中。 Similarly, by "above (above)", it should be understood that this is not only present in such definition of gravity, but also present in the pictogram defined above, such as a surface substantially perpendicular to the. 在一个实施例中,光学识别部件可以从上方由光学传感器或替换型装置来读取。 In one embodiment, the optical recognition means or may be replaced by the optical sensor type apparatus for reading from above. 而且,光学识别装置可配置在标记上,标记也许粘附地固定到特定载玻片或试剂瓶上。 Moreover, optical recognition device may be configured on the mark, marking perhaps adhesively fixed to a specific slide or reagent bottle. 借此,标记、也许是粘附标记可呈现给载玻片和试剂瓶上方的机器人头上的光学传感器装置,从而容易进行光学识别装置的读取。 Thereby, the marker, perhaps adhesion tag may be presented to the robot head optical sensor device above the slides and reagent bottle, thereby easily reading optical recognition device. 通过在固定到载玻片上的印刷标记上配备光学识别装置,试剂瓶、各个标记可在现场分别制备,并且可以将相关数据输入到计算机内,而携带所述相关数据的相应标记可在相关的标记印刷机上进行印刷。 By fixed to the slide of the printed indicia with optical recognition apparatus, reagent bottles, each marker may be prepared in the field, respectively, and the relevant data can be entered into the computer, and carries the respective associated data in the relevant tag printing presses on the mark.

在本发明的一个实施例中,一种类型的光学识别部件是,诸如所谓的“InfoglyphTM”型的二维高分辩率符号码。 In one embodiment of the present invention, a type of optical recognition component is a high-resolution two-dimensional, such as so-called "InfoglyphTM" type of symbol number. 光学识别还可以是更通用的二维符号。 Optical identification may also be more common two-dimensional symbols. 二维符号可以是以下数据的表示形式,包括(但不限于):组织样品相关数据、病人识别数据、染色规程数据、试剂相关数据、试剂种类数据、试剂体积相关数据、试剂有效期相关数据、以及类似数据。 A two-dimensional representation of the symbol may be of the following data, including (but not limited to): a tissue sample related data, patient identification data, protocol data staining, reagents related data, the reagent type data, data related to the volume of the reagent, the reagent is valid data, and Similar data. 通过将相关信息编码成众多微小的各个图表元素(一般是象0.02mm(1/100英寸)那么短的45斜线的短线),可获得具有高对比度编码信息标记的高分辩率,所述标记可以在印刷机上印刷,由高分辩率照相机读取。 By the relevant information encoded into many tiny individual chart elements (typically as 0.02mm (1/100 inch) as short as 45 slash short-term), having a high-resolution high-contrast coding information flag, the mark on the printing presses can be read by a high-resolution camera. 可以用不同的颜色和各种材料来提供这种类型的2-D符号标记。 You can use different colors and a variety of materials to provide this type of a 2-D symbols.

或者是,光学识别装置或替换型光学识别部件可以是数据矩阵码、甚至一维条形码,即具有垂直条图案的识别码,所述条的宽度和间隔识别所标记的项目。 Alternatively, an optical recognition apparatus or replace optical recognition means may be a data matrix code, or even one-dimensional barcodes, i.e., a vertical stripe pattern identification code, identifying the width and spacing of the marked items. 使用能够读取2-D符号的光学传感器的优点是,装置能够读取任何种类的光学标识符,因为仅需要对处理所捕获的也许是数字图象的软件做些改动即可。 The advantage of using 2-D symbol can read an optical sensor, the device can read any kind of optical identifier, because of the need to deal with only the captured digital image may be some changes to the software.

在一个实施例中,光学识别部件标记可包括二维(2-D)符号区以及甚至至少一个人可读取的文本区,每个如图5中所示意性表示的。 In one embodiment, the optical mark recognition component may comprise a two-dimensional (2-D) symbol area, and even the text area at least one person can read each Fig. 5 schematically represented. 借此,可由操作者对标记进行额外的目视检查,以便验证印刷标记。 In this way, by the operator to mark additional visual inspection to verify the printed mark.

在2-D图象捕获能力的更先进的用途中,图象处理能力或图象处理器部件适合识别由光学传感器捕获的组织样品自身的纹理或轮廓,并且可以用所述的图象捕获组织特性作为组织样品的个体标示。 In the 2-D image capturing ability of more advanced applications, the image processing capability or the image processor means for identifying tissue samples captured by the optical sensor itself texture or profile, and the image can be captured tissue characteristics of individual tissue samples as marked. 光学传感器可以构造成能够识别组织样品的所需特性,例如(但不限于)组织样品的纹理、轮廓、目视特性、甚至个体特征。 The optical sensor may be configured to be able to identify the desired properties of a tissue sample, such as (but not limited to) tissue sample texture, contour, visual characteristics, even individual characteristics. 当然,多个不同的特征或特性都可按需来识别,以便检测、或许识别包括任何属性、特征等的特性。 Of course, a plurality of different features or characteristics can be identified as needed, in order to detect, identify and perhaps include any attributes, characteristics of properties. 该实施例利用作废的载玻片标记作为自身的组织纹理,或者它的至少一个预定部分(有或者没有放大)用作控制软件中的数据表的标识。 This embodiment utilizes a slide labeled obsolete organization as its own texture, or it's at least a predetermined portion (with or without amplification) to identify the software used to control the data table.

在本发明的一个优选实施例中,光学传感器是可移动的光学传感器,其可沿染色及试剂部分上方的区域移动,所述光学传感器适合确定载玻片在至少一个染色部分的存在和位置。 In a preferred embodiment of the present invention, the optical sensor is movable optical sensor, which is movable along a portion of the top area of the stain and the reagent, the optical sensor adapted to determine the presence and location of slides at least one dye moiety. 通过拥有响应或也许在机器人部件上移动的光学传感器,可容易地实现这一点。 Or perhaps by having the robot in response to movement of the optical sensor unit can easily accomplish this. 一旦将一组新的载玻片装载到装置内,此特性就使得按照本发明该实施例的染色装置自动确定载玻片的位置,以便计算处理步骤的最佳进度。 Once a new set of slides were loaded into the apparatus, so that this feature is automatically determined slides dyeing device according to the embodiment of the invention the position, in order to calculate the optimal schedule processing step. 光学传感器甚至确定组织样品的大致部位和大致区域。 The optical sensor even determine approximate location and the approximate area tissue samples. 而且,此能力还可向装置提供控制软件,警告载玻片是否正确定位,或者在装载载玻片的过程中是否出现其它不规则定位。 Also, this capability also provides control software to devices, warning slides correctly positioned, or in the process of loading slides whether other irregular positioning.

在本发明的另一个实施例中,光学传感器适合定位预先选择的参比部位,以便对控制机器人头运动的机器人控制系统或机器人部件进行自校正。 In another embodiment of the present invention, an optical sensor adapted to be positioned pre-selected reference sites in order to control the movement of the robot head robot control system or robot components self-correction. 照相机用来指出校正系统所需的机器人臂的临界部位,从而使装置将机器人头定位到平台工作区内所有要求的位置和部位。 The camera is used to indicate the critical parts of the system for the correction of the robot arm, so that means the robot head is positioned to the platform work area location and position of all requirements. 如果由于诸如维修等,装置已经移动或损害,那么此特性就可使按照本发明的染色装置具有自动校正机器人运动控制系统的能力,诸如,通过检查滑轨是否正确装配在装置的接收部件内,和/或通过确定装置的预定参比部件的位置,来确定载玻片是否被安排在轨道内(预计宽泛地包含任何局部捆绑集合)。 If such maintenance, the device has been moved or damage, then this feature would enable the ability to automatically correct robot motion control system according to the dyeing apparatus of the present invention, such as, by checking whether the correct mounting rails in the receiving parts of the device, and / or by means of determining the position of a predetermined reference member, it is arranged to determine if the slides in the track (expected to broadly include any partial bundle collection).

在本发明的另一个实施例中,光学传感器可以是照相机,用来记录被操纵的组织样品的图象,该照相机可在所述组织样品经过一染色规程后记录最终组织样品的图象。 In another embodiment of the present invention, the optical sensor may be a camera for recording an image of the tissue sample is manipulated, the camera may be after a final recorded image staining procedure of tissue samples in the tissue sample. 在组织样品接受用于记录所操纵的组织样品的图象之后适合记录最终组织样品图象的照相机。 After the tissue samples were subjected to image tissue samples manipulated for recording suitable for recording the final tissue sample camera images. 借此,可以优选地以高分辨率记录已染色的组织样品的图面或数字图象,以便随后检查或将该数字化图面发送到远程的检查场所。 In this way, drawings or digital images with high resolution recording can be preferably dyed tissue samples for subsequent examination or the digitized drawings sent to the remote test site. 据此,在实施例中,本发明可以储存有关组织样品染色过程的图象。 Accordingly, in an embodiment, the present invention can store the image related to tissue sample dyeing process. 这当然可包括染色或一些其它操作之前和之后的图象。 This of course may include image dye or some other operation before and after. 而且,本发明的此特性可以将要染色或已经染色的组织样品图象存档,以便随后验证组织样品分析或标示是否是所需的。 Furthermore, this feature of the present invention can be dyed or stained tissue sample image has been archived tissue samples for subsequent analysis to verify whether it is required or indicate. 这样,本发明可以自动地使使用者的行为(例如上述那些)容易进行。 Thus, the present invention can automatically make the behavior of the user (e.g., those described above) easily. 为了弄懂多种可能性,自动容易进行的行为是指这样的行为,包括(但不限于):随后存取已染色的组织样品的组织图象、远程存取已染色的组织样品的图象、将已染色的组织样品的图象存档、随后存取未染色的组织样品的组织图象、远程存取未染色的组织样品的图象、将未染色的组织样品的图象存档、以及其它行为。 Then access the image has been stained tissue images of tissue samples, remote access has been stained tissue samples: In order to understand a variety of possibilities, easy to perform automatic behavior refers to behavior, including (but not limited to) , the dyed image filing tissue sample, followed by access to unstained tissue image of a tissue sample, a tissue sample image unstained remote access, the unstained tissue sample image filing, and other behavior.

在可以从一个或多个载玻片或试剂容器中自动识别信息的一些实施例中,配备传感器。 In some embodiments, the identification information automatically from one or more slides or the reagent container, with the sensor. 在一些实施例中,可以由适应性样品处理控制系统来提供方案信息。 In some embodiments, the program information may be provided by the adaptive control sample handling system. 样品处理系统潜在地按照由具有样品的载玻片提供或由适应性样品处理控制系统提供的方案信息,同时、依次或以任何其它临时方式处理一个或多个载玻片,或一批或多批载玻片。 Sample handling system in accordance with potentially provided by the slides have samples or samples provided by the adaptive process control system of the program information, simultaneously, sequentially or in any other way to deal with one or more temporary slides, or a group or Batch slide. 通过适应性样品处理控制系统完成的控制和监测,在处理方案步骤中可插入或除去成批样品或各个样品。 Control and monitoring via adaptive control system to complete sample handling, processing program step can be inserted or removed in bulk samples or individual samples.

可以达到本发明的前述或其它目的的本发明的另一实施例包括:一种样品处理方法,该方法包括以下步骤:接近多个中的至少一个抽屉(drawer);使至少一个样品载体止动组件具有至少一个样品载体;使至少一个抽屉具有至少一个样品载体止动组件;以及适当处理样品载体。 Another of the present invention can achieve the foregoing and other objects of the present invention embodiment includes: a sample processing method, the method comprising the steps of: at least one of the plurality close drawer (drawer); at least one sample carrier stopper carrier assembly having at least one sample; at least one sample carrier having at least one drawer stop assembly; and proper handling of the sample carrier. 适当处理步骤可自动处理样品并且可以连续或批处理载玻片,而且可以提供多个单独的载玻片处理,并且在一些实施例中可以提供丰余的载玻片处理,以便单独处理每个载玻片。 Appropriate processing steps can be processed automatically and continuously or batch sample slides, and slides may be processed to provide a plurality of individual, and in some embodiments may provide redundancy slides treated for separate treatment of each slide.

本发明的实施例还包括一种自动样品处理方法,该方法包括以下步骤:收集方案信息;将方案信息传送给至少一个样品处理系统;适当处理样品;以及从适当处理步骤中收集样品处理信息。 Embodiments of the present invention also includes an automatic sample preparation, the method comprising the steps of: collecting program information; transmitting scheme information to at least one sample processing system; appropriate treatment sample; and collecting the sample processing information from the appropriate process steps. 而且,实施例可提供:维护方案信息、维护样品处理信息、方案信息的信息共享以及样品处理信息。 Moreover, embodiments may provide: maintenance program information, maintain the sample processing information, information sharing program information, and sample information. 这些或其它方法步骤可提供各个样品或多批处理、样品诊断特性、以及多批处理的实时或适应性能力。 These or other methods or procedures provide each sample batch, a sample diagnostic features, as well as multi-batch, real-time or adaptive capacity.

本发明的许多方面可应用于免疫组织化学(IHC)技术,以及样品的原位杂交(ISH)和荧光原位杂交(FISH)特异性染色,还有微阵列,特别是包括样品的靶挽回(target retrieval)或染色的技术。 Many aspects of the present invention may be applied to immunohistochemistry (IHC) techniques, and in situ hybridization of the sample (ISH) and fluorescence in situ hybridization (FISH) staining specificity, as well as microarrays, especially to restore a sample comprising a target ( target retrieval) or dyeing techniques.

支持应该理解为是为本发明的以下方面和实施例而存在的:一种自动染色装置,包括:至少一个试剂容器;至少一个样品;适合影响所述试剂容器和所述样品的机器人部件;控制部件,所述机器人部件响应该控制部件;以及为了对所述自动染色装置中的至少一个部件进行二维成像,而构造的图象捕获2-D光学传感器。 Support should be understood that the following aspects and embodiments of the present invention exist: an automatic staining apparatus, comprising: at least one reagent container; at least one sample; Effect robot member for said sample container and said reagent; control means, responsive to the control means of the robot means; and for the automatic staining apparatus of at least one component of two-dimensional imaging, constructed in 2-D image capture optical sensor.

一种识别自动染色装置中的至少一个特性的方法,包括以下步骤:提供至少一个样品;提供至少一个试剂容器;提供适合影响所述试剂容器和所述样品的机器人部件;光学探测所述自动染色装置中的至少一个部件的二维图象;记录相关图象数据;以及将所述图象数据馈送到所述机器人部件响应的控制部件。 A method of identifying at least one characteristic of the automatic staining apparatus, comprising the steps of: providing at least one sample; providing at least one reagent container; means for providing the robot influence of the sample container and the reagent; optically detecting the Autostainer At least two-dimensional image of a part of the apparatus; recording relevant image data; and the image data will be fed to the robot control means responsive member.

一种在自动染色装置中染色样品的方法,包括以下步骤:提供至少一个样品;提供至少一个试剂容器;提供适合影响所述试剂容器和所述样品的机器人部件;提供响应所述机器人部件并适合探测所述自动染色装置中的至少一个部件的二维图象的光学传感器;记录相关图象数据;以及将所述图象数据馈送到所述机器人部件响应的控制部件。 A method for the automatic staining apparatus for staining a sample, comprising the steps of: providing at least one sample; providing at least one reagent container; means for providing the robot influence of the sample container and the reagent; provided responsive to said robot member and adapted detecting said automatic staining apparatus of at least one member of the two-dimensional image of the optical sensor; image recording relevant data; and the image data supplied to the control section of the robot part response.

一种自动染色装置,包括:至少一个试剂容器;至少一个样品;适合影响所述试剂容器和所述样品的机器人部件;控制部件,所述机器人部件响应该控制部件;以及为了探测所述自动染色装置中的至少一个部件而构造的多功能光学传感器。 An automatic staining apparatus, comprising: at least one reagent container; at least one sample; Effect robot member for said sample container and said reagent; control means, said robot means responsive to the control means; and for detecting the Autostainer Multifunctional optical sensor device in at least one component constructed.

附图简述以下参照附图来描述本发明,其中:图1是按照本发明优选实施例的染色装置的示意性透视图; BRIEF DESCRIPTION The following description of the present invention with reference to the accompanying drawings, in which: Figure 1 is a schematic perspective view of a dyeing device according to a preferred embodiment of the present invention;

图2是图1所示染色装置中的工作区域的附视图;图3是按照本发明一些实施例的染色装置中的机器人部件的细节图;图4是具有光学识别装置的试剂瓶的附视图;图5是其上具有光学识别标记的显微镜载玻片;图6是此标记布局的一个实例;以及图7-图10是载玻片上多种光学识别装置的实例。 Figure 2 is a dyeing apparatus shown in Figure 1 of the attached view of the work area; FIG. 3 is a dyeing device according to the present invention, the details of some embodiments of the robot member; Figure 4 is a view of a reagent bottle attached optical recognition apparatus ; FIG. 5 is an optical identification mark thereon having the microscope slide; FIG. 6 is an example of such a tag layout; FIGS. 7 and 10 are examples of a variety of optical recognition device on a glass slide.

本发明的实施方式图1和图2中表示出按照本发明的自动染色装置1。 Embodiments of the present invention Figure 1 and Figure 2 shows the invention of the automatic staining apparatus. 自动染色装置1包括围绕试剂站或试剂区域2的矩形框架4,以及第一和第二载玻片区域5。 Autostainer Reagent stations around the apparatus 1 comprises a rectangular frame 2 or the reagent zone 4, and the first region and the second slide glass 5. 试剂站或试剂区域2包括试剂瓶或容器室,其中每个室内放置有试剂瓶或试剂容器3,许多分离的轨道6放置在第一和第二区域5上,每个轨道6包括许多并排安装在轨道6内的显微镜载玻片7。 Reagents station 2 comprises a reagent or reagents area bottle or container chamber, wherein each reagent bottle placed indoors or reagent container 3, many of the isolated rail 6 is placed on the first and second regions 5, 6 each track includes a plurality of side by side microscope slides inside track 6 7. 多个试剂容器、甚至载玻片以任何所需的顺序(例如阵列)来放置。 A plurality of reagent containers, slides or in any desired order (e.g., arrays) to be placed. 在图示的实施例中,每个轨道可容纳8个载玻片,但是轨道可设计成能够容纳任何适当数目的载玻片。 In the illustrated embodiment, each track can hold eight slides, but the track may be designed to accommodate any suitable number of slides. 由于并排布置有8个轨道,因此图示实施例可容纳64个载玻片7,每个在载玻片的上面都固定有样品例如组织样品,因此试剂可从上方施加到每个载玻片上的样品中。 Since there are eight tracks arranged side by side, so the illustrated embodiment 7 can accommodate 64 slides, the slides are each in the above sample such as a tissue sample is fixed, so the reagent can be applied from above onto each slide samples. 所处理的样品可以是任何材料,但是最可能是生物材料例如生物样品或生物物种,也许是诸如组织样品例如组织或细胞物种、细胞、细胞集合或组织样品,定义包括细胞系、蛋白质及合成肽、组织、细胞前体、细胞制备品、血液、体液、骨髓、细胞学物种、血液涂片、薄层制备品和微阵列。 The treated sample may be any material, but most likely biological material such as a biological sample or biological species, such as may be tissue samples such as tissue or cell species, cells, cell or tissue sample collection, including the definition of cell lines, proteins and synthetic peptides , tissue, cell precursors, cells prepared products, blood, body fluids, bone marrow, cytology species, blood smears, thin-layer preparation and microarray products. 还应该理解,包括载玻片基生物样品。 It should also be understood that the biological sample comprises a slide glass substrate.

如上所述,本发明可包括在某种程度上影响试剂容器和组织样品的机器人部件。 As described above, the present invention may include a member in some way affect the robot reagent containers and tissue samples. 这样,对试剂容器或组织样品实施的任何类行为、由试剂容器或组织样品产生的行为或只是来自试剂容器或组织样品的信息,都可通过机器人部件容易地进行。 Thus, the behavior of any type or tissue sample reagent containers implemented by the reagent container or a tissue sample or just any behavior or tissue sample from the reagent container information, can be easily performed by a robot components. 机器人部件在实施例中,适合对施加有试剂等(包括作为结果或结合有)的载玻片进行染色。 In an embodiment, robot parts, suitable for applied reagents (including as a result of or in combination with) the slides were stained. 机器人臂或机器人部件20还可将试剂从试剂容器转移到预定的组织样品上。 Robot arm or robot part 20 can also transfer reagent from the reagent container to a predetermined tissue samples. 例如,用于沿X和Y(以及Z)方向(如箭头X和Y所示)移动探头10的机器人部件20,被安置在染色装置的框架4上方。 For example, a robot member (as indicated by arrows X and Y shown) for moving the probe along the X and Y (and Z) in the direction of 10 20, is arranged in the frame 4 above the dyeing apparatus. 机器人臂可以将探头10定位在所有试剂瓶3以及所有载玻片7的上方,并且进一步操纵探头10,以吸取任一瓶3中包含的部分试剂,从而将那部分试剂传送并施加到任一载玻片7上,以便对每个载玻片7上的样品有选择地进行染色和处理。 The robot arm may be positioned above the probe 10 all the reagent bottles 3, and all the slides 7, and further manipulate the probe 10 to partially absorb any reagent bottle 3 contained, so that part of the reagent delivery and applied to any slides 7 to selectively dyeing and processing of sample 7 on each slide. 控制部件通过控制染色过程的全部、甚至任何部分,来管理染色过程。 The control unit by controlling the entire dyeing process, or even any part to manage the dyeing process. 利用合适的控制部件或等同型控制装置(例如具有用于此目的的合适软件和输入数据的计算机(未示出)内的功能),该染色装置1能够对需要不同染色或处理试剂和过程的样品进行染色或处理。 Using suitable control means or equivalent type control device (e.g., having suitable software for this purpose and the input data of a computer (not shown function) within), the apparatus 1 is capable of staining require different staining or treatment reagents and processes The samples are dyed or treated.

如图1和3所示,探头10容纳在机器人头22中并由机器人部件20来操纵。 1 and 3, the probe 10 is accommodated in the robot head 22 in member 20 by a robot to manipulate. 探头10上升到上部位置(在Z方向),在此处清楚地看到,瓶3在探头10的下面,但是机器人可包括机器人头22中用于降低探头10的装置或部件,以便将探头尖浸到所选择的试剂瓶3的内容物中并吸取选定量的用于所选择的染色或处理过程的试剂。 Probe 10 rises to the upper position (in the Z direction), clearly seen here, the bottle 3 below probe 10, but the robot may include a robotic probe head 22 for reducing means or member 10, so that the probe tip immersed in the contents of the selected reagent bottle 3 and drawing a selected amount of reagent for the selected staining or treatment process. 在一个实施例中,本发明包括在机器人部件上配备光学传感器86,或许还包括通过机器人部件的动作将光学传感器移动到预定位置。 In one embodiment, the present invention includes an optical sensor 86 is equipped on the robot part, and perhaps by means of the operation of the robot is moved to a predetermined position of the optical sensor. 作为一个实例,机器人头22配有光学传感器86、甚至是指向下方的CCD照相机25。 As an example, the first robot 22 is equipped with an optical sensor 86, even below the CCD camera 25 point. 光学传感器位于机器人部件上、或许更广泛地响应机器人部件。 The optical sensor is located on the robot part, perhaps broader response robot parts. 在光学传感器定位之后,就在配置光学传感器的部位记录图象数据。 After the optical sensor positioning, image data is recorded in the configuration of the optical sensor site.

在一些实施例中,机器人部件20、甚至机器人头22可包括各种元件,包括(但不限于):可与汽缸39相连的推动工具38、可响应甚至与弹簧泵37相连的探头移动部件36的探头10、以及光学传感器86(如图3所示)。 In some embodiments, the robot part 20, the robot head 22 may even include various elements, including (but not limited to): pushing tool 39 can be connected with the cylinder 38, in response to the probe can be moved even with the spring member 37 connected to the pump 36 The probe 10, and an optical sensor 86 (Figure 3).

在实施例中,光学传感器可检测相关特性的二维图象数据。 In an embodiment, the optical sensor can detect two-dimensional image data related characteristics. 它还适合在总体上探测部件的二维图象。 It is also suitable for two-dimensional image on the whole probe member. 利用照相机确定载玻片和试剂瓶以及装置在工作区域内的其它特性的状态信息,例如阅读配置在试剂容器上的代码,以确定系统内的试剂种类和试剂部位。 Using a camera to determine the slides and reagent bottle and means other characteristics in the work area status information, such as reading the configuration code on the reagent container to determine the reagent type and reagent site system. 照相机还可确定组织样品载体的状态,例如特定玻片的部位、指示有关呈现在载玻片上的组织样品或待实施的处理方案的信息的信息索引例如代码。 The camera can also determine the status of a tissue sample carrier, e.g., a particular slide parts, indicating tissue sample on a slide presentation about the index information or the information processing scheme to be implemented, for example, code. 照相机可用于诊断目的。 The camera can be used for diagnostic purposes. 在一些实施例中,潜在地用计算机扫描样品,以便进一步进行分析。 In some embodiments, the computer scans the sample with potentially for further analysis. 本发明在实施例中可包括计算机图象生物分析部件、或许甚至是用计算机对样品的图象数据进行生物分析。 In an embodiment of the present invention may include a computer image analysis of biological components, perhaps even by computer image analysis of biological data samples.

如上所述,本发明可包括记录各种相关图象数据。 As described above, the present invention may include a record of all relevant image data. 当然,可记录各种相关图象数据。 Of course, you can record all relevant image data. 重要的是,这包括记录部件的校正参比点、或许甚至是装置上或装置内的机器人部件的校正参比位置。 Importantly, this includes calibration reference point recording means, perhaps even correcting the reference position of the robot part of the device or apparatus. 正如所提到的,本发明还可记录载玻片识别图象数据和试剂识别图象数据。 As mentioned, the present invention may further record the image data to identify slides and reagent identification image data. 一个实施例的显著方面是,可记录特定载玻片的光学识别部件、或许仅仅记录有关部件的信息。 A significant aspect of an embodiment is a recordable optical recognition component specific slides, perhaps only record information about the components. 这样的信息当然可以包括有关组织样品的信息。 Such information may include information about the course, the tissue sample. 同样,包括有关试剂容器内含有的试剂的信息的光学标识可记录在试剂容器上。 Similarly, including those related to the reagent container containing an optical identification information of some reagents can be recorded on the reagent container. 它可将二维符号记录在载玻片上、甚至试剂容器上。 It can record two-dimensional symbol on a glass slide, even on the reagent container. 记录在载玻片上的二维符号所代表的数据包括(但不限于):试剂相关数据、试剂种类数据、试剂体积相关数据、试剂有效期相关数据等。 Recorded on the two-dimensional symbols represented by data slide including (but not limited to): Reagent data, the data type of reagent, the reagent volume data, and other data related to the validity reagent. 它还可提供连接部件,所捕获的图象数据通过连接部件传送给控制部件。 It also provides connection means, the captured image data is transmitted to the control section via the connecting member. 它包括将图象数据馈送到控制部件,以便机器人部件可以响应。 Which comprises feeding the image data to the control section, so that the robot can respond member. 在记录相关图象数据之后、或许作为数据馈送到控制部件的结果,本发明可按照相关图象数据来操纵染色或其它过程。 After recording the relevant image data, perhaps as a result of the data fed to the control unit of the present invention may be used to manipulate the image data in accordance with the relevant dyeing or other processes. 这样,本发明可按照组织特异性方案来进行载玻片的染色。 Thus, the present invention can be carried out in accordance with tissue specific programs stained slides.

本发明的染色装置1还包括探头清洗站8和试剂混合器9,机器人部件20还进一步被设置成能够将探头运送到清洗站8以及试剂混合器9。 A dyeing apparatus of the present invention further comprises a reagent probe wash station 8 and the mixer 9, the robot member 20 is provided to be further transported to the probe washing station 8 and a reagent mixer 9.

如图4所示,试剂瓶3在安装有光学识别部件的表面上配有区域30。 4, the reagent bottle 3 on the mounting surface of the optical member with a recognition area 30. 此光学标识可以是携带有关瓶3的内容物的信息(例如试剂种类、制造数据、有效期限等)的粘附标记31。 This identification may be carrying optical information about the contents of the bottle 3 (e.g. the reagent type, manufacturing data, expiration date, etc.) adhesion numeral 31. 所编码的信息是数据矩阵码形式、Infoglyph码形式或2-D码的任何其它类型,也可能主要是简单的1-D码即条形码。 The information is encoded in the form of a data matrix code, any other form or type Infoglyph code 2-D code, it may be mainly simple 1-D bar code symbol that is. 此外,标记31还配有人可读的文本,以便诸如在将试剂瓶装载到染色装置内的过程中有助于操作者操纵试剂瓶。 In addition, numeral 31 also has a human-readable text, so as in the reagent bottle is loaded into the process of dyeing apparatus helps the operator manipulates the reagent bottle.

图5表示出其上安装有标记71的载玻片7。 Figure 5 shows markers mounted thereon slides 71 7. 图6中表示出标记71的一个布局。 Figure 6 shows a layout marker 71. 标记71可以是粘附的光学标识,是为特定载玻片制备的并在标记印刷机(未示出)或任何其它合适的印刷设备上进行印刷。 Mark 71 may be adhered optical identification, is prepared for a specific slide and press the mark (not shown) or any other suitable printing on printing equipment. 甚至在特定情形,如果一批载玻片接受相同的处理,则为这些载玻片提供一组相同的标记。 Even in certain instances, if the same number of slides to accept treatment, compared with a group of these slides offer the same reference numerals. 标记71包括用于有关载玻片7上组织样品的编码信息(例如病人数据、数据和文件号码、染色方案和/或处理步骤)的区域72。 Mark 71 includes encoded information about the tissue samples (such as patient data, and the file number, staining protocol and / or processing steps) on 7 area for 72 slides. 而且,标记71配有一行或多行人可读的文本和/或空白空间,以便制备载玻片的实验室人员在载玻片标记上进行书写。 Also, mark one or more pedestrian 71 with readable text and / or blank space, laboratory staff to prepare slides markers to write on a slide.

在图7-9中,表示出标记71的多种数据编码符号(如图所示的整个标记71,或者仅用于标记区域72(参见图6)。 In Figures 7-9, there is shown a plurality of data marks 71 coded symbols (as shown in the entire marker 71, or only for the mark region 72 (see FIG. 6).

在图7中,表示出InfoglyphTM型的2-D符号。 In Figure 7, showing InfoglyphTM type a 2-D symbol. 这包括或许甚至是信息型地毯型(carpet)符号。 This includes perhaps even informative type carpet (carpet) symbol. 这种2-D符号是有益处的,因为它能够携带大量的光学机器可读信息。 This 2-D symbol is beneficial, because it can carry a large number of optical machine readable information. 利用高分辨率照相机,这种类型的符号可以高分辨率来读取,并且大量信息编码在其中。 High resolution camera, this type of high resolution symbol may be read, and a lot of information encoded therein. 将此符号用不同方向、或许甚至不同颜色的微小斜线来印刷,并且能够用CCD照相机等容易地读取。 This symbol in different directions, and perhaps even different colors to print small slash, and can be easily read by a CCD camera or the like.

图8表示出用作Infoglyph符号替换形式的数据矩阵码。 Figure 8 shows an alternative form of symbols used Infoglyph data matrix code. 数据矩阵同样用CCD照相机来读取,但是可能不会象Infoglyph那样在编码中携带那么多的数据。 The same data is read by CCD matrix camera, but may not be as much data as carry Infoglyph in coding. 然而,如果载玻片和试剂瓶上需要的识别数据较少,则其就更容易印刷,因为它可具有不高的分辨率,从而是一种简单经济有效的解决方案。 However, if the slides and reagent bottles require less identification data, it is easier to print, because it may not have a high resolution, which is a simple and cost-effective solution. 另一种更简单的解决方案在图9中示出,其中符号是老的条形码。 Another, more simple solution is shown in Figure 9, which is the old bar code symbol. 在原理上这意味着,为了读取载玻片和试剂瓶的信息仅需要一个条形码扫描器,但是通过使用2-D传感器,自校正和监测载玻片的安装和在染色装置中的试剂的可能性就可以增强。 In principle this means that in order to read the information on the slides and reagent bottle requires only a bar code scanner, but by using 2-D sensor, self-tuning and monitoring of the installation of slides and reagents in the dyeing apparatus The possibility can be enhanced.

在一个实施例中,载玻片上和试剂瓶上的光学标示是同一类型。 In one embodiment, the optical mark on slides and reagent bottles are of the same type. 这可容易地对染色装置中的识别过程进行图象处理。 This device can be easily dyed in the identification process of the image processing.

一种识别各个载玻片的不同途径,或者是作为实施证实识别或储存证实信息的新能力的一种方式,可记录组织样品74本身的轮廓和/或纹理,例如如图10所示。 A different way to identify each slide, or as a way to identify or confirm embodiment of the new authentication information storage capacity, a tissue sample can be recorded contour 74 itself and / or texture, for example, as shown in FIG. 通过采用照相机能够记录的图象的高分辨率,组织样品本身的独特性质可用作载玻片的图表标示。 By using high-resolution camera capable of recording images of tissue samples themselves may be used as the unique nature of the chart labeled slides. 而且,能够记录已染色的组织样品的图象,从而产生组织样品的数字表示形式。 Further, an image can be recorded stained tissue sample, thereby generating a digital representation of the tissue sample. 该数字图象通过电子传送到用于立即检查的远程地方和/或为随后检查存档。 The digital image is transmitted to a remote place for immediate inspection and / or archived for subsequent examination by electrons. 这可使染色装置在使用上具有独特的灵活性,且可以引入新颖有益的组织样品分析方法。 This allows dyeing device has a unique flexibility of use, and can introduce new ways of organizing useful sample analysis.

除了识别染色装置中的显微镜玻片和试剂瓶之外,2-D光学传感器还能够用于装置的自校正,诸如在装置维修之后,如果装置已经拆卸或运送到另一个地方的话。 In addition to the recognition of a microscope slide staining apparatus and reagent bottles, 2-D optical sensor can also be used for self-correction devices, such as after the device repair, if the device has been removed or transported to another place of words. 通过用照相机捕获图象来识别装置内部的临界部位,图象处理软件可以将所捕获的图象与参比图象进行比较,以确定装置内的某些临界元件是否偏离它们的预定位置,诸如滑轨或玻片是否稍稍偏离,如果偏离,就计算机器人运动控制系统的一组校正数据,并将这组数据用于校正装置。 Capturing the image by the camera to identify the critical parts of the interior of the device, image processing software can be captured image is compared with the reference image to determine certain critical elements of the apparatus has deviated from their predetermined positions, such as slide or slide is slightly shifted, if deviation correction data to calculate a set of robot motion control system, and this set of data for correcting means. 如果所需的校正超过某一尺寸,就向操作者自动发出警告,从而确保装置在载玻片处理过程中不发生故障。 If the desired correction exceeds a certain size, to automatically warn the operator to ensure that the device does not malfunction processing slides. 而且,此图象分析系统还用于确定载玻片是存在于轨道上还是脱离轨道,以产生警告信号。 Moreover, the image analysis system is also used to determine the slides are present on the track or off track, in order to generate a warning signal.

通过本发明,应该意识到,在不脱离如权利要求书限定的本发明范围的前提下,可以对本发明一些优选实施例的上述描述作出各种改变。 By the present invention, it will be appreciated that, as in the appended claims without departing from the scope of the invention as defined in the premise, may some preferred embodiment of the present invention, the above described embodiments and that various changes. 正如所容易理解的,本发明的基本概念可以各种方式来实施。 As can be readily appreciated, the basic concept of the present invention may be implemented in various ways. 它包括染色技术以及用于完成染色和其它功能的多种系统、组件和设备。 It includes staining techniques and a variety of systems, components, and equipment for the dyeing and other functions. 在该申请中,染色技术也是作为用所述的多种系统、组件和设备来完成的所示部分结果以及作为固有采用的步骤来公开的。 In this application, the staining technique is shown in part as a result of a variety of systems, components, and equipment to complete the well as a natural step to open adoption. 它们应该被理解为,采用预计和描述的设备的自然结果。 They should be understood as a natural consequence of the use of the equipment is expected and described. 此外,虽然公开了一些设备,但是应该理解,这些不仅完成了一些方法,而且还能够用其它变换的方式来实施。 Further, although some devices are disclosed, it should be understood that these methods not only complete, but also can use other ways to implement the transformation. 重要的是,对于上述所有内容,所有这些方面都应该理解为包括在本发明的公开文本中。 It is important for all of the above, all of which should be understood to include the disclosure of the present invention.

读者应该意识到,具体论述可能不会非常清楚地描述所有可能的实施例;许多可以选择的例子都是示意的。 The reader should be aware that the specific discussion may not be very clearly describe all possible embodiments; many examples are illustrative of the option. 它还不能充分解释本发明的总的性质,不能明白地表示每一特性或部件是如何实际代表更广泛功能或各种替换型或等同部件的。 It can not fully explain the general nature of the invention, it can not clearly indicate how each feature or component is actually representative of a broader function or various alternative type or equivalent parts. 再者,这些都明显包括在此公开文本中。 Again, these are obviously included in this disclosure. 当本发明使用一些术语重点来描述设备时,设备的每个部件都明显行使一个功能。 When the focus of some of the terms used herein to describe the device when, for each piece of equipment are obviously a function of the exercise. 对于所述设备不仅包括装置权利要求,而且为了实现本发明和每个部件实施的功能,还包括方法或工艺权利要求。 The apparatus comprises a means for not only the claim, and in order to implement the present invention and the functions implemented in each member, further comprising a method or process claims. 无论是说明书还是术语都无意限制本公开文本的范围。 Both terms are not intended to limit the specification or the scope of the present disclosure.

还应该理解,在不脱离本发明本意的前提下可以做出各种变化。 It should also be understood that the present invention without departing from the intended premise that various changes may be made. 这些变化也都明显包括在本说明书中。 These changes are also evident included in the description. 它们还落在本发明的范围内。 They also fall within the scope of the invention. 包括图示的示范性实施例、各种含蓄替换型实施例以及广泛的方法或工艺等的泛泛公开内容都包含在本公开文本中,并且据此用以支持本专利申请或随后专利申请的附加权利要求。 Including the exemplary embodiments illustrated, various subtle and widespread replacement of the embodiment of a method or process, etc. are included in the general disclosure of this disclosure, and accordingly to support this patent application or a subsequent additional patent applications claims.

而且,本发明及权利要求的每个部件还可用各种方式来实现。 Also, each member and the claims of the present invention may also be required to use a variety of ways. 本公开文本应理解为包括每个这样的变型,即任何装置实施例、方法或工艺实施例、或者仅仅是其中任何部件的变型。 This disclosure should be understood to include each such variation, i.e. cases, the method or process embodiment any apparatus embodiment, wherein the variant or simply any component. 尤其是,应该理解为,当本公开文本涉及本发明的部件时,每个部件的词汇可以由等同的装置词汇或方法词汇来表达,即使仅仅功能或结果是一样的。 In particular, it should be understood that when the present disclosure relates to a part of the present invention, each vocabulary component can be expressed by the words or equivalent means method vocabulary, even if only the function or result is the same. 这样的等同、延伸、甚至更多派生词应该认为是包括在每个部件或行为的描述中。 Such equivalent, extending, or more derivatives should be considered included in the description of each member or the behavior. 当期望解释本发明所涉及的广泛范围时,这样的词汇是可替代的,其中需要解释授予本发明的隐含宽泛范围。 When the desired interpretation broad scope of the invention, such a word is an alternative, which implied the need to explain the broad scope of the present invention is granted. 但是作为实例,应该理解,所有的行为都可表达为实施该行为的方式或产生该行为的部件。 But as an example, it should be understood that all behavior can be expressed as a way to implement the act or produce parts of the act. 同样,所公开的每个物理部件应该理解为包括物理部件所实施的行为的公开内容。 Similarly, each physical member disclosed should be understood to include physical components of the embodiment of the disclosure act. 关于最后这方面,作为一个实例,“传感器”的公开应该理解为包括“探测”行为的公开,而无论是否是明显论述,反之,如果有“探测”行为的有效公开,这样的公开就应该理解为包括“传感器”、甚至“探测方式”的公开。 Concerning this last aspect, as an example, "sensor" should be understood to include disclosure "probe" behavior public, regardless of whether it is clearly discussed, on the contrary, if there is a valid public "probe" behavior, it should be understood that such disclosure include "sensor" and even "detection methods" in public. 还应该理解,在具体语言可直译为限定作用的权限中,作为美国的一个实例,其中对部件“装置(means for)”的一些解释被限制窄了,而更宽泛的等同语言(例如“部件”等)可用来避免窄的解释,并且应该理解为被本说明书所包含。 It should also be understood that, in the specific language can be literally translated as defining the role of authority in the United States as an example, in which the member some explanation "means (means for)" is limited to a narrow, while the broader language equivalents (such as "member ", etc.) can be used to avoid a narrow interpretation, and should be understood to be included in the present specification. 这样的变化和等同词汇应该理解为明显包括在此描述中。 Such variations and equivalents should be understood to significantly vocabulary included in this description.

本专利申请中提到的任何专利、专利申请、公开出版物或其它参考文献在此处都作为参考并入本文。 Any patents, patent applications, publications, or other references mentioned in this patent application are herein incorporated by reference herein. 此外,对于所用的每个词汇,应该理解,除非其在本申请中的使用与这些解释不符,否则通用的词典定义都应该理解为是针对每个词汇的,并且诸如the Random House Webster's Unabridged Dictionary(第二版)中包含的所有定义、等同词汇和同义词,在此都作为参考并入本文。 In addition, for each of the terms used, it should be understood that, unless its use in this application is inconsistent with these explanations, otherwise common dictionary definitions should be understood to be for every word, and as the Random House Webster's Unabridged Dictionary ( The second edition all definitions) contained in the equivalent words and synonyms in this are incorporated herein by reference. 最后,任何本申请的以前案件都附属在此并且作为参考并入本文。 Finally, before any of this application in this case they are affiliated and incorporated herein by reference.

这样,这些申请应该理解为对至少以下权利要求是支持的:i)正如本文所公开和描述的,每个样品处理系统和子系统,ii)所公开和描述的相关方法,iii)每个这些系统、组件、设备和方法的类似、等同、甚至是隐含的变型,iv)正如所公开和描述的,完成所示每个功能的那些替换型设计,v)完成所示每个功能的那些替换型设计和方法,vi)作为单独和独立发明示出的每个特征、元件和步骤,vii)由所公开的多种系统或元件增强的应用,viii)由这样的系统或元件产生的最终产品,以及ix)基本上作为此前描述的以及参照任何附带实例的方法和系统、组件、设备及装置,x)所公开的每个部件的多种组合及变换,xi)正如附加在所表现的每个独立权利要求和概念上的,每个潜在的从属权利要求,xii)如上所述的,借助于计算机实施的方法,xiii)如上所述的,可编程的系统,xiv)如上所述的,用数据编码的计算机可读存储器,所述数据用于指导包括行使功能的装置或部件的计算机,xv)象本文所公开和描述的那样构造的计算机,xvi)如本文所公开和描述的,单个或组合压规程和程序,xvii)所公开和描述的相关的方法,xviii)这些每个系统和方法的相似、等同、甚至是明显的变型,xix)正如所公开和描述的,完成所示每个功能的那些替换型设计,xx)完成所示每个功能的那些替换型设计和方法,xxi)作为单独和独立发明示出的每个特征、元件和步骤,xxii)以上每个发明的多种组合及变换。 Thus, these applications should be understood as at least the following claims are supported by: i) As disclosed and described herein, each sample handling systems and subsystems, ii) related methods disclosed and described, iii) each of these systems Those Alternatively, similar components, devices and methods, equivalent, and even implicit variations, iv) as disclosed and described herein, each function as those shown complete replacement design, v) is completed as shown in each function design and methods, vi) as a separate and independent invention shows each feature, component, and step, vii) final products from a variety of enhanced systems or components disclosed in the application, viii) by such systems or components produced and ix) a variety of combinations and converting each component as well as reference to any incidental substantially instance methods and systems, components, equipment and apparatus described previously, x) as disclosed, xi) As demonstrated in each additional independent claims and conceptual potential of each dependent claim, xii) described above, by means of a computer-implemented method, xiii) as described above, a programmable system, xiv) as described above, data encoded with computer readable memory, including the data used to guide the exercise of the function of the device or part of a computer, xv) as disclosed herein and as described computer configuration, xvi) as herein disclosed and described, single or a combination of pressure protocols and procedures, xvii) related methods disclosed and described, xviii) similar to each of these systems and methods, equivalents, and even obvious variant, xix) As disclosed and described, as done in each Replace those design a functional, xx) that the completion of the replacement design and method shown in each function, xxi) as a more separate and independent invention shows each feature, elements or steps, xxii) each of the above invention combinations and permutations.

另外,如果使用或在使用时,按照传统的权利要求解释,过渡词“包括”等的使用,在本文是用来维持“开放式”权利要求的。 In addition, if you use or in use, according to the traditional interpretation of claims, a transitional word "comprising", etc. used in this paper is used to maintain the "open" claims. 这样,除非上下文有其它要求,否则应该理解,词汇“包括(comprise)”或诸如“包括(comprises)”或“包括(comprising)”等的变型,都意指暗示包括规定部件或步骤或部件或步骤的组合,但不排除任何其它部件或步骤或部件或步骤的组合。 Thus, unless the context other requirements, otherwise it should be understood that the word "include (comprise)" or as "includes (comprises)" or "including (comprising)" and other modifications are meant imply the inclusion of a predetermined part or parts, or steps or combinations of steps, but not the exclusion of any other combination of elements or steps or components or steps. 这样的词汇用其最广泛的形式来解释,以便为本申请提供最宽泛的法律许可的范围。 Such vocabulary with its broadest form to explain to the present application provides the broadest extent permitted by law.

所提出的任何权利要求必然作为本发明描述的一部分以参考文献的形式并入本发明,并且申请人清楚地保留用这些权利要求的所有或一部分组合内容作为额外说明书描述的权利,以便支持任何或全部权利要求或其任何部件或元件,申请人还清楚地保留将这些权利要求的任何部分或全部组合内容或其任何部件或元件从说明书的描述中移到权利要求内的权利,或者反之亦然,以便规定本申请或其任何随后连续申请、分案申请或部分连续申请所寻求的保护主题,或者以便在追踪或符合任何国家或条约的专利法、规则或规章的费用方面得到减少的好处,并且以参考文献的形式并入的这些内容应该在本申请的整个待审过程中有效,包括其任何随后该案的连续申请、分案申请或部分连续案的申请或任何再公告或基于其上的延伸申请。 Any right that the claim is inevitable as part of the present invention will be described in the form of which is incorporated herein by reference, and the applicant clearly right to use all or part of a combination of these claims as content specification describes additional reserves in order to support any or all the claims or any element or component, the applicant also reserves the right to clear the contents of all combinations of any part of these claims or any component or components thereof move claimed in claims from the description in the specification, or vice versa , to provide for the protection of the subject of this application, or any subsequent continuous application, a divisional application or part of the continuous application sought, or to obtain a reduction in the cost of benefits tracking or any national or treaty patent law, rules or regulations respect, and incorporated by reference in the form of these elements should be effective in the present application pending the entire process, including any subsequent continuous application of the case, a divisional application or part of the case of continuous application or any further announcement or based on The extension request.

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