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Publication numberCN1726275 A
Publication typeApplication
Application numberCN 200380106521
PCT numberPCT/US2003/040518
Publication date25 Jan 2006
Filing date19 Dec 2003
Priority date20 Dec 2002
Also published asCA2507960A1, CA2507960C, CA2508000A1, CA2508001A1, CA2508070A1, CA2508113A1, CA2508323A1, CA2508323C, CA2508370A1, CA2511032A1, CA2974221A1, CN1726386A, CN1955711A, CN100424166C, CN100472197C, EP1572979A1, EP1572979A4, EP1573297A1, EP1573311A2, EP1573311A4, EP1573312A2, EP1573312A4, EP1573408A2, EP1573408A4, EP1576376A2, EP1576376A4, EP1579190A1, EP1579190A4, EP1579194A2, EP1579194A4, US7400983, US7648678, US7758809, US7937228, US7960178, US8216512, US8257968, US8298815, US8386195, US8394635, US8529836, US8663978, US8673642, US8784735, US8788217, US8969086, US9040284, US9229016, US9599630, US20040266015, US20050064535, US20060045806, US20060046298, US20060063265, US20060085140, US20060088928, US20060088940, US20060172426, US20080241876, US20100081167, US20110167930, US20110269238, US20120310422, US20130029409, US20130084567, US20130203103, US20130217108, US20130330252, US20140038232, US20140186218, US20140234170, US20140286838, US20140356935, US20160084862, WO2004057307A1, WO2004058404A2, WO2004058404A3, WO2004058950A1, WO2004059284A2, WO2004059284A3, WO2004059287A2, WO2004059287A3, WO2004059288A2, WO2004059288A3, WO2004059297A1, WO2004059441A2, WO2004059441A3
Publication number200380106521.5, CN 1726275 A, CN 1726275A, CN 200380106521, CN-A-1726275, CN1726275 A, CN1726275A, CN200380106521, CN200380106521.5, PCT/2003/40518, PCT/US/2003/040518, PCT/US/2003/40518, PCT/US/3/040518, PCT/US/3/40518, PCT/US2003/040518, PCT/US2003/40518, PCT/US2003040518, PCT/US200340518, PCT/US3/040518, PCT/US3/40518, PCT/US3040518, PCT/US340518
Inventors道格斯威特, 马克基, 戈登法因格尔德, 克里斯托弗布坎南, 鲍勃莱思罗普, 约翰法武齐
Applicant达可斯通(丹麦)公司
Export CitationBiBTeX, EndNote, RefMan
External Links: SIPO, Espacenet
Method and apparatus for auto-dyeing of tissue slides
CN 1726275 A
Abstract  translated from Chinese
本发明涉及一种组织样品染色装置(1)。 The present invention relates to a tissue sample dyeing device (1). 所述装置(1)包括试剂部分(2)或试剂容器(3);至少一个染色部分或组织样品;可将试剂移到预定组织样品中的机器人头(22)或机器人部件(20),所述机器人部件(20)可以在试剂和染色部分的上方移动;可以管理染色过程的控制部件(85);用来检测相关特性的二维图像数据并将所捕获的图像数据馈送到控制部件(86)的2-D光学传感器。 The device (1) comprises a reagent portion (2) or the reagent container (3); at least a part or stain tissue samples; reagent can be moved to a predetermined tissue sample robot head (22) or a robot part (20), the said robot member (20) can be moved over the reagent and the staining section; can manage the control means (85) the dyeing process; correlation characteristics for detecting two-dimensional image data and captured image data is fed to the control member (86 ) of 2-D optical sensor. 通过提供具有2-D光学传感器(86)的机器人部件(20),可提供具有多功能的公共图像处理器。 By providing the robot member (20) having a 2-D optical sensor (86), providing a common image processor versatile. 通过采用2-D光学图像处理系统,装置的控制系统可容易地适合读取多种类型的数据表达形式,从而可以识别装置部分的实际图像,以便存取装置的条件。 By using 2-D optical image processing system, a control system apparatus can be easily adapted to read the expression in the form of a plurality of types of data, which can identify the actual image portion of the apparatus, so that the access conditions of the device. 光学传感器用来自动识别装置中存在的载玻片和试剂容器,因此光学传感器可用于检查载玻片是否从载玻片位置错位或缺失等。 The optical sensor is used to automatically identify the slide and reagent present in the container means, so the optical sensor can be used to check whether the slide glass from the slide glass misplaced or missing location.
Claims(34)  translated from Chinese
1.一种自动染色装置,包括:至少一个位于试剂部分内的试剂容器;至少一个样品;至少两个被被所述试剂部分分开的染色部分;适合影响所述试剂容器和所述样品的机器人部件;所述机器人部件响应的控制部件;以及为了对所述自动染色装置中的至少一个部件进行二维成像,而构造的图象捕获2-D光学传感器。 An automatic staining apparatus, comprising: at least one reagent container is located inside the reagent portion; at least one sample; at least two portions separated by said staining reagent part; for the influence of said reagent and said sample container robot member; and a control means in response to said robot member; and for the automatic staining apparatus of at least one component in a two-dimensional image, and configured to capture 2-D image optical sensor.
2.根据权利要求1所述的装置,其特征在于,所述光学传感器适合定位预选的用于自校正所述机器人部件的参比特征。 2. The apparatus according to claim 1, characterized in that the optical sensor is adapted for positioning a preselected reference feature correction since the components of the robot.
3.根据权利要求1或2所述的装置,其特征在于,所述光学传感器适合在所述样品经受染色方案之后记录最终的样品图像。 1 or 3. The apparatus according to claim 2, characterized in that the optical sensor is suitable for recording the final image of the sample after the sample is subjected to staining protocol.
4.根据权利要求1所述的装置,其特征在于,至少一个部件包括从由以下部分构成的组合中选择的要素:二维高分辨率符号码、数据矩阵码、条形码、粘附标记、二维符号区和人可读的文本区;并且所述所述光学传感器适合在所述样品经受染色方案之后记录最终的样品图像。 4. The apparatus according to claim 1, characterized in that at least one component comprises elements selected from the group consisting of the following parts: a high-resolution two-dimensional symbol code, Data Matrix code, barcode, adhesion mark, two dimensional symbols region and a human-readable text area; and wherein the optical sensor for recording the final image of the sample after the samples were subjected to staining solution.
5.根据权利要求1所述的装置,其特征在于,所述光学传感器被构造成能够识别从以下特征构成的组合中选择的特征:样品的纹理、样品的轮廓、样品的可视特性、以及样品的个体识别特征;并且所述光学传感器适合在所述样品经受染色方案之后记录最终的样品图像。 5. The apparatus according to claim 1, characterized in that the optical sensor is configured to be able to identify the following characteristics selected from the group consisting of wherein: visual properties of the texture sample, the outline of the sample, the sample, and individual identification characteristic of the sample; and the optical sensor is suitable for recording the final image of the sample after the sample is subjected to staining protocol.
6.一种识别自动染色装置中的至少一个特性的方法,包括以下步骤:提供至少一个样品;提供至少一个试剂容器;提供适合影响所述试剂容器和所述样品的机器人部件;光学探测所述自动染色装置中的至少一个部件的二维图象;记录相关图象数据;记录所述装置中的机器人部件的校正参比点;以及将所述图象数据馈送到所述机器人部件响应的控制部件。 6. A method of identifying at least one characteristic of the automatic staining apparatus, comprising the steps of: providing at least one sample; providing at least one reagent container; means for providing the robot affect the sample container and the reagent; optical detection of the At least two-dimensional image of an automatic dyeing apparatus member; record relevant image data; recording calibration reference point of the apparatus components of the robot; and feeding said image data to said robot control means responsive components.
7.一种在自动染色装置中染色样品的方法,包括以下步骤:提供至少一个样品;提供位于轨道内的载玻片;提供至少一个试剂容器;提供适合影响所述试剂容器和所述样品的机器人部件;提供响应所述机器人部件并适合探测所述自动染色装置中的至少一个部件的二维图象的光学传感器;记录相关图象数据;记录所述轨道的机器人部件的校正参比位置;以及将所述图象数据馈送到所述机器人部件响应的控制部件。 A method of staining the sample in an automatic dyeing apparatus, comprising the steps of: providing at least one sample; providing located inner rail slides; providing at least one reagent container; to provide for the impact of the reagent container and the sample robot parts; provided responsive to said robot member and an optical sensor for detecting two-dimensional image of the automatic staining apparatus of at least one component; record relevant image data; correcting the reference position of the recording of the track components of the robot; and to feed the image data to the robot control means responsive member.
8.一种自动染色装置,包括:至少一个试剂容器;至少一个样品;适合影响所述试剂容器和所述样品的机器人部件;所述机器人部件响应的控制部件;以及适合定位用于自校正机器人部件的预选参比特征的光学传感器。 An automatic staining apparatus, comprising: at least one reagent container; at least one sample; for the influence of said reagent and said sample container robot member; said control means in response to the robot member; and a suitable location for self-correcting robot member preselected reference characteristic optical sensor.
9.一种自动染色装置,包括:至少一个在试剂部分的试剂容器;至少一个包含在第一载玻片部分的载玻片上的第一样品;至少一个包含在第二载玻片部分的载玻片上的第二样品,其中所述第一载玻片部分与所述第二载玻片部分被所述试剂部分分开;适合影响所述试剂容器和所述第一及所述第二样品的机器人部件;以及所述机器人部件响应的控制部件。 An automatic staining apparatus, comprising: at least one reagent in the reagent container portion; at least one included in the first portion of the sample slide on the first slide glass; at least one included in the second part of the slide glass the second sample on the slide, wherein the first portion and the second slide glass portion is separated from the slide glass reagent portion; reagent container and the fit of the first and the second sample and control means responsive member of the robot; robot member.
10.一种自动染色装置,包括:至少一个试剂容器;至少一个样品;适合影响所述试剂容器和所述样品的机器人部件;所述机器人部件响应的控制部件;以及图像捕获2-D传感器,所述传感器被构造成能够给所述自动染色装置内的至少一个部件二维成像,其中所述至少一个部件包括具有重复信息的光学识别部件。 An automatic staining apparatus, comprising: at least one reagent container; at least one sample; for affecting said robot member said reagent container and the sample; and a control means in response to said robot member; and an image capture 2-D sensor, the sensor is configured to enable the automatic staining apparatus to said at least one component of the two-dimensional image, wherein said at least one component comprises an optical recognition unit having duplicate information.
11.根据权利要求10所述的装置,其特征在于,所述重复信息包括多重复信息。 11. The apparatus according to claim 10, wherein said information comprises a plurality of repeating duplicate information.
12.根据权利要求10所述的装置,其特征在于,所述重复信息包括冗余信息。 12. The apparatus according to claim 10, wherein said information comprises repeating redundant information.
13.根据权利要求10、12所述的装置,其特征在于,所述至少一个部件包括光学识别部件。 13. The apparatus according to claim 10, 12, characterized in that said at least one component comprises an optical recognition unit.
14.根据权利要求11所述的装置,其特征在于,所述光学识别部件包括二维高分辨率符号码。 14. The apparatus according to claim 11, wherein said optical component comprises a two-dimensional high-resolution character identification number.
15.根据权利要求11所述的装置,其特征在于,所述光学识别部件包括数据矩阵码。 15. The apparatus according to claim 11, wherein said identification means comprises an optical data matrix code.
16.根据权利要求11所述的装置,其特征在于,所述光学识别部件包括条形码。 16. The apparatus according to claim 11, wherein said optical component comprises a bar code identification.
17.一种自动染色装置,包括:至少一个试剂容器;至少一个样品;适合影响所述试剂容器和所述组织样品的机器人部件;所述机器人部件响应的控制部件;图像捕获2-D传感器,所述传感器被构造成能够给所述自动染色装置内的至少一个部件二维成像;以及计算机图像生物分析部件。 17. An automatic staining apparatus, comprising: at least one reagent container; at least one sample; robot part for the impact of the reagent container and the tissue sample; and a control means responsive member of the robot; image capturing 2-D sensor, the sensor is configured to enable the automatic staining apparatus to said at least one component of the two-dimensional image; and computer image analysis of biological components.
18.根据权利要求17所述的装置,其特征在于,所述光学传感器包括照相机。 18. The apparatus according to claim 17, characterized in that the optical sensor comprises a camera.
19.根据权利要求18所述的装置,其特征在于,所述照相机包括CCD部件。 19. The apparatus according to claim 18, characterized in that said camera comprises a CCD member.
20.根据权利要求17所述的装置,其特征在于,所述样品包括容纳在载玻片上的生物样品。 20. The apparatus according to claim 17, wherein said sample comprises a biological sample on a glass slide accommodated.
21.一种识别自动染色装置中的至少一个特性的方法,包括以下步骤:提供至少一个样品;提供至少一个试剂容器;提供适合影响所述试剂容器和所述样品的机器人部件;光学探测所述自动染色装置中的至少一个部件的二维图象;记录相关图象数据;将所述图象数据馈送到所述机器人部件响应的控制部件;以及用计算机对所述至少一个样品的图像数据进行生物分析。 21. An automatic staining apparatus identifying at least one characteristic of the method, comprising the steps of: providing at least one sample; providing at least one reagent container; means for providing the robot affect the sample container and the reagent; optical detection of the At least two-dimensional image of an automatic dyeing apparatus member; record relevant image data; to be supplied to said image data in response to the control section of the robot member; and a computer for the image data of said at least one sample were bioanalysis.
22.根据权利要求21所述的方法,其特征在于,所述光学探测所述自动染色装置中的至少一个部件的二维图象的步骤,包括采用照相机的步骤。 22. The method according to claim 21, wherein said step of two-dimensional image of the optical detection of the automatic staining apparatus of at least one component, comprising the step of using a camera.
23.根据权利要求22所述的方法,其特征在于,所述采用照相机的步骤,包括采用CCD部件的步骤。 23. The method according to claim 22, wherein said step of using a camera, comprising the step of using CCD member.
24.根据权利要求21、22或23所述的方法,其特征在于,所述提供至少一个样品的步骤,包括采用载玻片的步骤。 21, 22 or 24. The method according to claim 23, wherein said step of providing at least one sample, comprising the step of using a glass slide.
25.一种在自动染色装置中染色样品的方法,包括以下步骤:提供至少一个样品;提供至少一个试剂容器;提供适合影响所述试剂容器和所述样品的机器人部件;提供响应所述机器人部件并适合探测所述自动染色装置中的至少一个部件的二维图象的光学传感器;记录相关图象数据;将所述图象数据馈送到所述机器人部件响应的控制部件;以及用计算机对所述至少一个样品的图像数据进行生物分析。 25. A method of staining the sample in an automatic dyeing apparatus, comprising the steps of: providing at least one sample; providing at least one reagent container; means for providing the robot affect the sample container and the reagent; providing in response to said robot member and for detecting two-dimensional image of the automatic staining apparatus of at least one member of an optical sensor; record relevant image data; feeding said image data to said control means in response to the robot member; and with the computer said at least one sample image data of biological analysis.
26.根据权利要求25所述的方法,其特征在于,所述提供至少一个样品的步骤,包括采用载玻片的步骤。 26. The method according to claim 25, wherein said step of providing at least one sample, comprising the step of using a glass slide.
27.根据权利要求25或26所述的方法,其特征在于,所述提供光学传感器的步骤,包括采用照相机的步骤。 27. The method according to claim 25 or claim 26, wherein said step of providing an optical sensor, comprising the step of using a camera.
28.根据权利要求25或26所述的方法,其特征在于,所述提供光学传感器的步骤,包括采用CCD部件的步骤。 28. The method according to claim 25 or claim 26, wherein said step of providing an optical sensor, comprising the step of using CCD member.
29.根据权利要求25所述的方法,其特征在于,还包括储存有关组织样品染色过程的图像的步骤。 29. The method according to claim 25, characterized in that, further comprising the step of storing image related tissue samples of the dyeing process.
30.一种自动染色装置,包括:至少一个试剂容器;至少一个样品;适合影响所述试剂容器和所述样品的机器人部件;所述机器人部件响应的控制部件;被构造成能够探测所述自动染色装置中的至少一个部件的多功能光学传感器;以及计算机图像生物分析部件。 30. An automatic staining apparatus, comprising: at least one reagent container; at least one sample; for the influence of said reagent and said sample container robot member; said control means in response to the robot part; can be configured to automatically detect the analysis and computer image of the biological part; multifunctional optical sensor means at least one dye component.
31.根据权利要求30所述的装置,其特征在于,所述至少一个样品包括容纳在载玻片上的至少一个样品。 31. The apparatus according to claim 30, characterized in that said at least one sample comprising at least one sample on a glass slide accommodated.
32.根据权利要求30所述的装置,其特征在于,所述光学传感器包括照相机。 32. The apparatus according to claim 30, characterized in that the optical sensor comprises a camera.
33.根据权利要求30所述的装置,其特征在于,所述光学传感器包括CCD部件。 33. The apparatus according to claim 30, characterized in that the optical sensor comprises a CCD member.
34.根据权利要求30所述的装置,其特征在于,还包括有关组织样品染色过程的储存图像。 34. The apparatus according to claim 30, characterized in that, further comprising a tissue sample dyeing process relevant storage image.
Description  translated from Chinese
组织样品自动染色方法和装置 Tissue samples staining method and apparatus for automatic

技术领域 FIELD

本发明涉及一种组织样品自动染色的装置和方法。 The present invention relates to an apparatus and method for automatic staining of tissue samples. 本发明进一步涉及用于样品处理以及样品处理的数据收集、数据维护和数据检索的系统。 The present invention further relates to a sample handling and sample handling data collection, data storage and data retrieval system. 本发明特别涉及的应用领域包括免疫组织化学、原位杂交、荧光原位杂交、特异性染色和细胞学,以及潜在的其它化学和生物学应用。 The present invention particularly relates to application areas include chemical, in situ hybridization, fluorescence in situ hybridization, specific staining and cytology, as well as potentially other chemical and biological applications of immunohistochemistry.

背景技术 BACKGROUND

免疫组织化学(IHC)应用和其它化学和生物学分析(例如原位杂交、特异性染色和细胞学)中的组织样品处理,要求一个或多个处理次序或规程作为一个或多个样品分析的一部分。 Immunohistochemistry (IHC) applications and other chemical and biological analysis (e.g., in situ hybridization, and staining cytological specificity) in the tissue sample, require one or more processing sequences or protocols as one or more sample analysis part of it. 这些样品处理次序或规程是由要求分析的个体或组织(例如医院的病理学家和组织学家)规定的,或者由待实施的特定分析指令(例如由组织规定的标准方案)来规定。 These samples were processed by the requirements of the order or regulation is analyzed individual or organization (such as a hospital pathologist and histologist) regulations, or to be implemented by specific analysis instructions (such as the standard program provided by the organization) to specify.

在样品分析的制备中,生物样品可以通过公知的样品获取技术来取得,并且可以包括在一些应用中甚至是一个或多个分离的细胞的组织。 In the preparation of samples analyzed, the biological sample can be obtained by known techniques to obtain a sample, and may include in some applications even one or more cells of the tissue separation. 组织样品可容纳在样品载体(例如载玻片、或者也许是显微镜载玻片)上。 Tissue samples may be contained in the sample carrier (e.g. a glass slide, or perhaps a microscope slide) on.

例如,免疫应用需要包括诸如脱蜡、靶回收和染色这些步骤的处理次序或规程。 For example, the application needs to include such immunity dewaxing, processing order or regulation of target recovery and staining of these steps. 以前,在一些应用中,这些步骤由人工来实施,因此潜在地导致这是一个耗时的规程并且需要人员主动参与到样品处理中。 Previously, in some applications, these steps manually implemented, thus potentially leading to it is a time consuming procedure and requires personnel to actively participate in the sample processing. 尤其是关于染色过程,多种组织载玻片的自动染色设备是公知的,因为为了满足对有利样品处理以及几乎不用人工烦琐操作的需求,已经对样品处理的自动化作了一些尝试。 Particularly with regard to the dyeing process, a variety of automatic staining tissue slides are well-known devices, because in order to meet the demand for enabling virtually no manual sample handling and cumbersome operation of automated sample processing has made some attempts.

本发明的各个方面尤其可应用于具有一个或多个处理步骤的样品处理中,所述处理步骤是在一个样品、一部分或全部样品上进行的,这些规程在一些情况下是由呈现样品的各个载体来识别的。 Aspects of the present invention is particularly applicable to samples treated with one or more processing steps, the processing step is in a sample, a part or all of the samples, and in some cases these procedures are presented for each sample by a vector to recognize. 本发明的各个方面尤其可应用于免疫组织化学(IHC)技术、以及原位杂交(ISH)和荧光原位杂交(FISH),特别是包括样品染色的技术。 Aspects of the present invention is particularly applicable to immunohistochemistry (IHC) techniques, and in situ hybridization (ISH) and fluorescence in situ hybridization (FISH), especially including sample dyeing technique.

本发明的实施例进一步涉及样品处理的自动控制系统。 Embodiments of the present invention further relates to an automatic sample processing control system. 这些实施例还涉及样品处理的数据收集、数据维护、数据检索,特别是处理规程和处理状态的信息分享,例如各个样品或多批处理、样品诊断特性、以及多批处理的实时或适应能力。 These embodiments also relates to data processing of the sample collection, data maintenance, data retrieval, in particular, the processing procedures and processing status information sharing, such as the respective sample or batch, sample diagnostic features, as well as multi-batch or real-time adaptability.

US5,839,091公开了一种组织自动染色的装置,其中将显微镜载玻片布置成许多行,而将试剂瓶储存在毗邻此载玻片部分的部分中。 US5,839,091 discloses an automatic tissue staining apparatus, wherein the microscope slide is arranged in a plurality of rows, and the reagent bottle is stored in the next section of this portion of the slide glass. 机器人头按照控制程序从瓶中拾取预定量的试剂,将这些量的试剂沉积在预定的载玻片上,并吹散载玻片上的液体。 The robot head in accordance with a predetermined control program from the bottle pick-up amount of reagent, the amount of these reagents will be deposited on the predetermined slides and blow away the liquid on the slide glass. 此程序在与染色装置偶连的计算机上运行。 This program runs on a computer with dual staining device connected. 染色装置装载有许多载玻片,每个载玻片及其位置都登记在计算机中,并选择染色次序。 Staining apparatus loaded with a lot of slides, each slide and its location are registered in the computer, and select staining order. 该程序还接收有关试剂及其在试剂部分上的位置的数据。 The program also receives data about the agent and its location on the reagent portion. 基于这些载玻片和试剂位置数据,程序计算染色运行并控制机器人在染色装置中的运动。 Based on these slides and reagents position data, the program calculates the dyeing operation and control of the robot movement in the dyeing apparatus.

US6,352,861公开了一种转盘型自动染色装置,其中将载玻片布置在可旋转的转盘式载玻片支持体上,将试剂同样布置在载玻片支持体上方的可旋转的转盘式试剂支持体上。 US6,352,861 discloses an automatic dial-type dyeing apparatus, in which the slides are arranged on a rotatable carousel slides the support, the same reagent is arranged above the slide glass body is rotatably supported by a reagent carousel support body. 然后将特定载玻片旋转到输送区,将特定的试剂瓶也旋转到该位置,试剂被分配到载玻片上。 The slides were then rotated to convey the specific area, a specific reagent bottle is also rotated to this position, the reagent is assigned to the slide. 载玻片和试剂瓶配有条形码,且提供相关的条形码读取器来分别识别载玻片和试剂。 Slides and reagent bottle with a bar code, and provide a bar code reader to identify the slide and reagent, respectively. 吹散区和识别区也配备在载玻片转盘的四周。 Disperse the area and the identification zone is also equipped with slides around the turntable. 载玻片条形码读取器识别载玻片样品及其特定样品所需的特定免疫组织化学过程。 Slides barcode reader to identify a particular sample slides and samples required for a specific immunohistochemical procedure. 定位试剂条形码读取器,以扫描试剂瓶上的试剂条形码。 Positioning the reagent barcode reader to scan the reagent bottle on the reagent barcode. 来自载玻片条形码读取器和试剂条形码读取器的扫描信息被馈送到计算机内,并分别与载玻片和试剂转盘的索引位置相关。 Scan information from the slides and reagent barcode reader bar code reader is supplied to the computer, and are associated with the index position of the slide and the reagent turntable. 此信息用来旋转载玻片转盘和试剂转盘,以便为每个载玻片的每个载玻片处理步骤将正确的试剂瓶定位在分配区中。 This information is used to rotate the turntable and reagent carousel slides to the correct location in the allocation area reagent bottle for each slide processing steps of each slide.

'091中描述的自动染色装置的缺陷是,载玻片部分和试剂部分的每个组织载玻片和每个试剂瓶的位置,必须分别人工输入到计算机中,这是因为控制程序不能检查特定载玻片和试剂瓶的部位。 'Defect Autostainer apparatus 091 is described, the position of the slide glass portion and the reagent portion of each tissue slides and each reagent bottle, must be manually inputted into the computer, because the control program can not check a specific part of the slide and reagent bottles. 这具有用错误的染色规程处理错位载玻片并且染色装置使用非常不灵活的危险。 It has a deal with the wrong procedure stained slides and stained misplaced device using very inflexible danger.

虽然'861专利采用了载玻片和试剂的条形码识别,但是这种转盘型装置在运行染色规程时是耗时的,因为这涉及到对于规程中的每个步骤,要用全部试剂存货旋转转盘,并用所有的载玻片旋转载玻片转盘。 Although the '861 patent uses a bar code identification slides and reagents, but the dial-type device when running staining procedures are time-consuming because it involves procedures for each step, to use all reagents inventory rotating turntable and rotate the turntable slides with all slides. 这些旋转是耗时的,并使得这种类型的装置不适合运行大量的载玻片。 These rotations are time-consuming, and makes this type of device is not suitable for running a large number of slides. 而且,条形码仅能携带少量数据,一般是简单的识别码,这意味着控制计算机必须配有有关识别码的相应数据。 Moreover, the bar code can only carry small amounts of data, usually a simple identification code, which means that the control computer must be equipped with the appropriate data relating to the identification code.

发明的公开内容本发明的一个目的是,提供一种开始提到的那种组织样品的自动处理装置,这种装置能够自动识别机器中存在的试剂和载玻片存货。 An object of the present invention is the disclosure of the present invention is to provide automatic processing apparatus of a kind of tissue samples mentioned at the beginning, which means the machine can automatically identify the presence of reagents and slides inventory. 另一个目的是,提供对装置相关特性的识别,以便在新装载的载玻片的染色过程启动之前,进行自动制备检查。 Another object is to provide identification of the relevant characteristics of the apparatus, so that the slide glass before the dyeing process the newly loaded start, automatic inspection prepared.

在一个实施例中,这些目的是通过开始提到的那种装置实现的,其中机器人部件(也许具有机器人头)配有光学传感器(或者也许是2-D光学传感器装置),所述传感器用于检查相关特性的二维图象数据并具有将所捕获的图象数据馈送到控制装置的能力。 In one embodiment, the object is achieved by apparatus of the kind mentioned at the beginning of the implementation, where the robot member (perhaps having a robot head) is equipped with an optical sensor (or maybe 2-D optical sensor device), the sensor is used Check-correlation characteristics and the two-dimensional image data having the captured image data supplied to the control device capabilities.

本发明还提供了一种识别自动染色装置中的至少一个特性的方法,所述染色装置也许包括至少一个载玻片阵列和试剂阵列,以及也用试剂实施载玻片染色的机器人部件(或者也许是机器人装置);所述方法在一个实施例中包括以下步骤:在机器人装置的机器人头上配备光学传感器装置,将所述机器人头上的光学传感器装置移动到预定位置,记录所述位置的相关图象数据,以及将所述图象数据馈送到控制系统,以便根据所述图象数据操纵染色过程。 The present invention also provides a method of identifying the automatic staining apparatus of at least one characteristic of the method, the staining apparatus may include at least one glass slide array and an array of reagent, and a reagent for the implementation of the robot member also slides stained (or perhaps the robot apparatus); the method in one embodiment comprises the steps of: the head of the robot in the robot apparatus is provided with an optical sensor device, the optical sensor means for moving the robot head to a predetermined position related to the position of the recording image data, and to feed the image data to the control system, in order to manipulate the image data according to the dyeing process.

而且,本发明涉及一种在自动染色装置中染色组织样品的方法,所述染色装置也许包括至少一个载玻片阵列和试剂阵列,以及按照组织样品的特异性染色规程也用试剂实施载玻片染色的机器人部件(或者也许是机器人装置);所述方法在一个实施例中包括以下步骤:在机器人装置的机器人头上配备光学传感器装置,将所述机器人头上的光学传感器装置移动到预定位置,用所述光学传感器装置记录所述位置的相关图象数据;将所述图象数据馈送到控制系统,以便根据所述图象数据操纵染色过程;以及也利用来自试剂容器的试剂给组织样品染色。 Moreover, the present invention relates to a method in an automatic staining apparatus stained tissue samples, the staining apparatus may include at least one array slides and reagents array, and in accordance with the procedures of the tissue sample specific staining reagent of embodiment also slides Robot member dyed (or perhaps robot apparatus); In one embodiment, the method comprises the steps of embodiment: the head of the robot in the robot apparatus is provided with an optical sensor device, the optical sensor means for moving the robot head to a predetermined position , using the optical sensor means for recording the position of the relevant image data; to feed the image data to the control system, in order to manipulate the dyeing process according to the image data; and also by using a reagent from the reagent container to a tissue sample staining.

在实施例中,自动染色装置1(是用至少一些自动操作染色的任何装置)可包括试剂容器3。 In an embodiment, the automatic staining apparatus 1 (with at least some of any automated staining apparatus) may include a reagent container 3. 一个或多个试剂容器3可位于自动染色装置1的试剂部分2。 One or more reagent reagent container 3 may be located partially automated staining apparatus 1 of 2. 自动染色装置还可包括在此处可位于载玻片上的组织样品74。 Automatic staining apparatus may further comprise a tissue sample herein may be located on the slide 74. 其上具有组织样品的多个载玻片也可位于自动染色装置1的载玻片部分5。 Having a plurality of slides on which tissue sample may also be located on a slide glass automatic staining apparatus 1 part 5. 本发明在实施例中还可包括机器人部件20、一些类型的控制部件、甚至光学传感器(86),也许是图象捕获2-D光学传感器。 In an embodiment of the present invention may also include a robot part 20, some type of control means, even an optical sensor (86), perhaps the image capture 2-D optical sensor. 正如所容易理解的,控制部件85可以是计算机、软件程序、或者仅仅是特定的可编程的处理器功能。 As can be readily appreciated, the control member 85 may be a computer, a software program, or just a particular programmable processor functions.

如上所述,本发明能够光学探测二维图象。 As described above, the present invention is capable of optically detecting two-dimensional image. 这可通过图像捕获2-D传感器来实现,所述传感器在自动染色装置1中可提供部件的二维图象。 This is achieved by the image capturing 2-D sensors to achieve, in the sensor in the automatic staining apparatus 1 can provide two-dimensional image member. 通过提供具有2-D光学传感器或装置的机器人部件(也许是机器人头),正如在一个实施例中,公共图象处理装置能够具有多个功能。 By providing the robot member having a 2-D optical sensor or device (perhaps a robot head), as in one embodiment, the common image processing apparatus capable of having a plurality of functions. 通过利用2-D光学图象处理系统,染色装置的控制系统可容易地适合读取多种数据表示形式,由此部件或者是装置部分的实际图象一样,从而能够评估装置的状况。 By using 2-D optical image processing system, the control system can be easily dyed apparatus for reading a variety of data representations, whereby the member or part of the actual image of the same apparatus, it is possible to assess the status of the device. 光学传感器或光学传感器装置可用来自动识别装置中存在的载玻片和试剂容器,由此光学传感器或光学传感器装置可用来检查载玻片是否在某一载玻片位置错位或缺失等。 An optical sensor or an optical sensor device may be used to automatically identify the slide and reagent present in the container means, whereby an optical sensor or an optical sensor device may be used to check whether the slides in a slide glass misplaced or missing location.

光学传感器使按照本发明的染色装置具有迄今不可预见的灵活性并且能够使染色装置中的识别功能自动化。 The optical sensor having flexibility hitherto unforeseen dyeing device according to the present invention and the dyeing apparatus enables automatic identification function. 通过也许在机器人头、甚至是机器人部件上采用CCD-照相机等,可以不再需要用于每个识别任务的各个识别装置。 Perhaps by the robot head, even using CCD- camera on the robot parts, etc. may be no longer required for each of the respective identification means recognition task. 这意味着,对装置的控制和维修容易进行。 This means that, for the control and maintenance of the device easier. 控制染色装置的软件适合包括装置的多种特性和状况(包括载玻片和试剂信息)的自动识别。 Control staining apparatus including software for automatic identification of a variety of features and status means (including slides and reagent information). 通过利用识别染色装置中相关信息的方法和实施按照本发明的染色过程的方法,自动染色过程几乎不耗时且包括更多的定性检查,而在载玻片的染色操作中不会显著损失速度。 Dyeing apparatus by using identification information about the implementation of the method and method according to the dyeing process of the present invention, the automatic dyeing process is hardly more time-consuming and include qualitative examination, and dyeing the slide glass without significant loss in operating speed .

在本发明的一个实施例中,试剂部分容纳多个试剂容器,这些容器固定布置成多行。 In one embodiment of the present invention, the reagent portion accommodating a plurality of reagent containers, which are arranged into a plurality of fixed lines. 同样,组织样品容纳在载玻片上,这些载玻片在染色过程中在至少一个染色部分或在玻片部分固定布置成多行。 Similarly, tissue samples were housed on a slide, which slides in the dyeing process in dyeing, at least one part of the fixed part or the slide is arranged in a plurality of rows. 这些部分的布局使得其代表机器人头的基本上平坦的平台工作区,此工作区可以在X-轴和Y-轴上移动。 Layout which represents these portions such that the robot head is substantially flat platform workspace, the workspace can be moved in the X- and Y- axes. 在一个特别优选的实施例中,一行载玻片和/或试剂被除去或更换,而不会干扰染色过程。 In a particularly preferred embodiment, a line slides and / or reagent is removed or replaced without disturbing the dyeing process.

在另一个优选实施例中,染色装置包括至少两个被试剂部分分开的染色部分,即它们布置成使至少一些组织样品靠近至少一些试剂容器。 In another preferred embodiment, the staining apparatus comprising at least two separate portions staining reagent portion, i.e., they are arranged so that at least some of the tissue sample is close to at least some of the reagent container. 借此,可以显著限制机器人头为了到达所有载玻片而需要的运动,并借此增大染色装置的容量,由此可减少运行染色规程的时间或得到其它优点。 In this way, you can significantly limit the movement of the robot head in order to reach all the slides and needs, and to increase the capacity of dyeing apparatus, thereby reducing the running time of staining procedures or get other advantages. 进一步意识到,利用这种载玻片和试剂部分的布局还可达到更短的处理时间或其它优点,而无需可视系统例如光学传感器。 Further appreciated that using this arrangement reagent portion of the slide and also to achieve a shorter processing time or other advantages, such as an optical system without visual sensor.

在本发明的其它实施例中,光学传感器可以是照相机,或者也许包括CCD部件。 In other embodiments of the present invention, the optical sensor may be a camera, or may include CCD member. 通过词汇“照相机”,应该理解,可以采用任何图象捕获装置,而无论是薄膜、板、存储器,还是任何类型的电子介质,而无论其成象的是光、可视电磁辐射,或者甚至是诸如现在公知的不可视的电磁辐射。 By the term "camera" is to be understood, any image capture device, and whether it is a film, plate, memory, or any type of electronic media, regardless of imaging light, visible electromagnetic radiation, or even now known as non-visible electromagnetic radiation. 通过记录相关图象、相关图象数据、甚至记录数字图象数据,利用公知的已经可用的图象处理能力,可以快速的方式在控制系统中完成该数据的计算机处理。 By recording the relevant image, associated image data, and even recording digital image data, using a known image processing capabilities already available, can be quickly accomplished computer processing of the data in the control system. 而且,通过利用该数字技术,能够用高分辨率记录相对复杂的图象,由此在机器人头连续穿过载玻片标记时,可实现对几个识别(例如一整行载玻片上的标记)的快速记录,于是可避免每个载玻片识别的终止时间和开始时间。 Further, by using the digital technique, it is possible to use relatively complex image resolution record, whereby when the robot head slides through continuous marking can be realized on several identification (e.g., a whole line mark on the slide) fast recording, so to avoid identification of each slide end time and start time. 然而,通过本发明,意识到,根据图象解析的需要,还可采用其它图象传感器例如固态传感器,或者也许是CMOS传感器。 However, by the present invention, realized according to the image resolution required, other image sensors may be employed such as solid state sensors, or perhaps is a CMOS sensor.

如上所述,光学传感器适合记录装置中存在的各个试剂容器或试剂瓶以及载玻片。 As described above, the optical sensor means for recording the presence of individual reagent containers or reagent bottles and slides. 虽然其可给更大的区域、甚至整个设备成像,但是它可以构造成以电子、光学或定位的方式单独进行成象。 Although it may be to a larger area, or even the entire image forming apparatus, but it can be configured to electronic, optical or positioning imagewise manner separately. 无论如何,作为成象能力的结果,无需装载到自动染色装置内的载玻片或试剂容器或试剂瓶的预定位置,因为载玻片和试剂瓶一旦装载到装置内,装置就适合自动识别新的载玻片和试剂瓶。 In any event, as a result of imaging capabilities, without loading the slide or the predetermined positions of the reagent containers or reagent bottles automatic staining apparatus, because once the slides and reagent bottle loaded into the apparatus, the apparatus is adapted to automatically recognize the new slides and reagent bottles.

在一个实施例中,试剂容器和载玻片配有光学识别部件。 In one embodiment, the reagent containers and slides with the optical recognition component. 例如,试剂容器可配有试剂光学识别部件,载玻片可配有载玻片光学识别部件。 For example, the reagent container can be equipped with optical recognition reagent components, the slides can be equipped with optical recognition component slides. 这些光学识别部件可包含有关试剂种类的机器可读的数据以及有关瓶内试剂的其它相关数据,载玻片识别部件可含有有关组织样品的数据,例如病人的识别、染色规程等。 Other relevant data which may contain information about the optical recognition component reagent type of machine-readable data and relevant reagent bottle, slides recognition component may contain data relevant tissue sample, such as identifying, dyeing procedure the patient and the like. 光学识别部件可包括重申信息、甚至冗余信息。 Optical recognition component may include reiterated information, even redundant information. 这可包括重复、甚至部分重复的信息,并且甚至包括可以是或者可以不是有关类似信息的不同译本的信息。 This may include repeat, and even some duplicate information, and may or may even include different translations of the information is not related to similar information.

光学识别部件或者替换型光学识别装置可以光学识别部件能够由光学传感器读取的方式位于、甚至安装在试剂容器上或玻片上。 Optical identification of components or replace optical recognition device can identify the part of the optical optical sensor can be located by the way read, or even mounted on glass slides on the reagent container. 通过位于“之上(on)”,预计任何相关的方式都能够包括;由此应该理解,无需单独的固定或表面安装。 By located "above (on)", is expected to have any relevant way can include; should thus be appreciated that no separate fixed or surface mounting. 同样,通过在“上方(above)”,应该理解,这不仅存在于诸如有关重力的定义中,而且还存在于诸如大致垂直地在表面等上方的象形定义中。 Similarly, in the "up (above)", it should be understood that this is not only present in the medium such as a definition of gravity, but also in the pictogram is defined over the surface or the like in such a substantially vertically. 在一个实施例中,光学识别部件可以从上方由光学传感器或替换型装置来读取。 In one embodiment, the optical recognition unit or can be replaced by an optical sensor type device to read from above. 而且,光学识别装置可配置在标记上,标记也许粘附地固定到特定载玻片或试剂瓶上。 Moreover, optical recognition device may be configured on the tags, which may adhesively secured to a particular slide or reagent bottles. 借此,标记、也许是粘附标记可呈现给载玻片和试剂瓶上方的机器人头上的光学传感器装置,从而容易进行光学识别装置的读取。 Thereby, the mark may be adhered tag may be presented to the robot head of the optical sensor device above the slides and reagent bottles, thereby easily reading optical identification device. 通过在固定到载玻片上的印刷标记上配备光学识别装置,试剂瓶、各个标记可在现场分别制备,并且可以将相关数据输入到计算机内,而携带所述相关数据的相应标记可在相关的标记印刷机上进行印刷。 By fixed to the slide of the printed indicia with optical recognition means, reagent bottles, each mark can be prepared in the field, respectively, and the relevant data can be entered into the computer, and carrying the associated data corresponding indicia can be associated printing marks on the printing press.

在本发明的一个实施例中,一种类型的光学识别部件是,诸如所谓的“InfoglyphTM”型的二维高分辩率符号码。 In one embodiment of the present invention, one type of optical identification means is two-dimensional high-resolution, such as the so-called "InfoglyphTM" type of symbol codes. 光学识别还可以是更通用的二维符号。 Optical recognition can also be more general two-dimensional symbols. 二维符号可以是以下数据的表示形式,包括(但不限于):组织样品相关数据、病人识别数据、染色规程数据、试剂相关数据、试剂种类数据、试剂体积相关数据、试剂有效期相关数据、以及类似数据。 A two-dimensional representation of the symbol may be the following data, including (but not limited to): a tissue sample related data, patient identification data, protocol data staining, reagents related data, the reagent type data, data related to the reagent volume, the reagent is valid data, and similar data. 通过将相关信息编码成众多微小的各个图表元素(一般是象0.02mm(1/100英寸)那么短的45斜线的短线),可获得具有高对比度编码言息标记的高分辩率,所述标记可以在印刷机上印刷,被高分辩率照相机读取。 Through relevant information encoded into many tiny individual chart elements (usually as 0.02mm (1/100 inch) shorter then 45 slash short-term), obtained with a high-resolution high-contrast information encoded word mark, the reference mark on the printing presses can, read high-resolution camera. 可以用不同的颜色和各种材料来提供这种类型的2-D符号标记。 Can use different colors and various materials to provide this type of 2-D symbol.

或者是,光学识别装置或替换型光学识别部件可以是数据矩阵码、甚至一维条形码,即具有垂直条图案的识别码,所述条的宽度和间隔识别所标记的项目。 Alternatively, an optical recognition device or replace optical recognition means may be a data matrix code, or even one-dimensional barcode, i.e., a vertical bar pattern having an identification code identifying the strip width and spacing of the marked items. 使用能够读取2-D符号的光学传感器的优点是,装置能够读取任何种类的光学标识符,因为仅需要适应处理所捕获的也许是数字图象的软件即可。 The advantage of using 2-D symbols can read an optical sensor is that any kind of device capable of reading an optical identifier, since only the need to adapt the processing of the captured digital image may be a software.

在一个实施例中,光学识别部件标记可包括二维(2-D)符号区以及甚至至少一个人可读取的文本区,每个如图5中所定义性表示的。 In one embodiment, the optical mark recognition component may comprise a two-dimensional (2-D) symbol area and the text area or the at least one person can be read, each as defined in Figure 5 showing the. 借此,可由操作者对标记进行额外的目视检查,以便验证印刷标记。 Whereby, marked by the operator additional visual inspection, in order to verify the printed indicia.

在2-D图象捕获能力的更先进的用途中,图象处理能力或图象处理器部件适合识别由光学传感器捕获的组织样品自身的纹理或轮廓,并且可以用所述的图象捕获组织特性作为组织样品的个体标示。 In the 2-D image capturing ability of the more advanced applications, the image processing capacity, or the image processor means is adapted to identify a tissue sample captured by the optical sensor itself texture or contour, and can be said image capture tissue characteristics of individual tissue samples as marked. 光学传感器可以构造成能够识别组织样品的所需特性,例如(但不限于)组织样品的纹理、轮廓、目视特性、甚至个体特征。 The optical sensor may be configured to be able to identify the desired characteristics of the tissue sample, such as (but not limited to) tissue sample texture, contour, visual characteristics, even individual characteristics. 当然,多个不同的特征或特性都可按需来识别,以便检测、或许识别包括任何属性、特征等的特性。 Of course, a plurality of different features or characteristics can be identified as needed, in order to detect, identify and perhaps include any attributes, characteristics of properties. 该实施例利用作废的载玻片标记作为自身的组织纹理,或者它的至少一个预定部分(有或者没有放大)用作控制软件中的数据表的标识。 This embodiment utilizes a slide labeled obsolete as its own tissue texture, or it's at least a predetermined portion (with or without amplification) to identify the software used to control the data table.

在本发明的一个优选实施例中,光学传感器是可移动的光学传感器,其可沿染色及试剂部分上方的区域移动,所述光学传感器适合确定载玻片在至少一个染色部分的存在和位置。 In a preferred embodiment of the present invention, the optical sensor is movable optical sensor, which is movable along the area above the staining and reagent section, said optical sensor adapted to determine the presence and location of slides in at least one dye moiety. 通过拥有响应或也许在机器人部件上移动的光学传感器,可容易地实现这一点。 By having or perhaps in response to movement of the robot part optical sensor, this can be easily realized. 一旦将一组新的载玻片装载到装置内,此特性就使得按照本发明该实施例的染色装置自动确定载玻片的位置,以便计算处理步骤的最佳进度。 Once a new set of slides were loaded into the apparatus, so that this feature is automatically determined according to stained slides of the embodiment of the invention means the position, in order to calculate the optimal schedule processing step. 光学传感器甚至确定组织样品的大致部位和大致区域。 Optical sensors determine the organization even approximate location and the approximate area of the sample. 而且,此能力还可向装置提供控制软件,警告载玻片是否正确定位,或者在装载载玻片的过程中是否出现其它不规则定位。 Moreover, this capability also provides means to control software, warning slides correctly positioned, or in the course of the loading slides whether other irregular positioning.

在本发明的另一个实施例中,光学传感器适合定位预先选择的参比部位,以便对控制机器人头运动的机器人控制系统或机器人部件进行自校正。 In another embodiment of the present invention, the optical sensor adapted to be positioned a pre-selected reference location, in order to control the robot head motion of the robot control system or robotic components can be aligned automatically. 照相机用来指出校正系统所需的机器人臂的临界部位,从而使装置将机器人头定位到平台工作区内所有要求的位置和部位。 The camera is used to indicate the critical portion of the system required for the correction of the robot arm, so that the robot head positioning means to the platform position and location of all the work area required. 如果由于诸如维修等,装置已经移动或损害,那么此特性就可使按照本发明的染色装置具有自动校正机器人运动控制系统的能力,诸如,通过检查滑轨是否正确装配在装置的接收部件内,和/或通过确定装置的预定参比部件的位置,来确定载玻片是否布置在轨道内(预计宽泛地包含任何局部捆绑集合)。 If such maintenance, means have been moved or damaged, then this feature would enable the ability to automatically correct the robot motion control system in accordance with the dyeing apparatus of the present invention, such as, by checking whether the correct rail member fitted in the reception apparatus, and / or by means of determining the predetermined position of the reference member, to determine whether the slide glass arranged on the inner rail (expected broadly include any partial bundle collection).

在本发明的另一个实施例中,光学传感器可以是,在组织样品接受用于记录所操纵的组织样品的图象之后适合记录最终组织样品图象的照相机。 In another embodiment of the present invention, the optical sensor may be, after tissue samples were subjected to image tissue samples manipulated for recording suitable for recording an image of the final sample tissue camera. 借此,可以优选地以高分辨率记录已染色的组织样品的图面或数字图象,以便随后检查或将该数字化图面发送到远程的检查场所。 Whereby drawing or digital image recording with high resolution can be preferably dyed tissue samples for subsequent examination or the digitized drawings sent to the remote test site. 据此,在实施例中,本发明可以储存有关组织样品染色过程的图象。 Accordingly, in an embodiment, the present invention can store the image related tissue sample dyeing process. 这当然可包括染色或一些其它操作之前和之后的图象。 This of course may include coloring or some other image before and after the operation. 而且,本发明的此特性可以将要染色或已经染色的组织样品图象存档,以便随后验证组织样品分析或标示是否是所需的。 Moreover, this feature of the present invention can be dyed or stained tissue sample images have been archived tissue samples for subsequent analysis or verification is required to indicate whether the. 这样,本发明可以自动地使使用者的行为(例如上述那些)容易进行。 Thus, the present invention can automatically cause the user's behavior (e.g., those described above) can be facilitated. 为了弄懂多种可能性,自动容易进行的行为是指这样的行为,包括(但不限于):随后存取已染色的组织样品的组织图象、远程存取已染色的组织样品的图象、将已染色的组织样品的图象存档、随后存取未染色的组织样品的组织图象、远程存取未染色的组织样品的图象、将未染色的组织样品的图象存档、以及其它行为。 Then access the image stained tissue sample of tissue images, remote access has been stained tissue samples: In order to understand a variety of possibilities, automatic behavior easy means such behavior, including (but not limited to) , the dyed image filing tissue sample, followed by access to image tissue unstained tissue samples, tissue samples can be remotely accessed image unstained, the unstained tissue sample image filing, and other behavior.

在可以从一个或多个载玻片或试剂容器中自动识别信息的一些实施例中,配备传感器。 In some embodiments, the identification information may be automatically slide or from one or more reagent containers, and with the sensor. 在一些实施例中,可以由适应性样品处理控制系统来提供方案信息。 In some embodiments, the program information may be provided by adaptive control sample handling system. 样品处理系统潜在地按照由具有样品的载玻片提供或由适应性样品处理控制系统提供的方案信息,同时、依次或以任何其它临时方式处理一个或多个载玻片,或一批或多批载玻片。 Sample handling system in accordance with potentially provided by slides having a specimen or sample handling provided by the adaptive control system scheme information, simultaneously, sequentially or in any other manner one or more temporary slides, or a group or Batch slide. 通过适应性样品处理控制系统完成的控制和监测,在处理方案步骤中可插入或除去成批样品或各个样品。 Control and monitoring of sample processing by adaptive control system is completed, the program in the processing step can be inserted or removed sample or batch of each sample.

可以达到本发明的前述或其它目的的本发明的另一实施例包括:一种样品处理方法,该方法包括以下步骤:接近多个中的至少一个抽屉(drawer);使至少一个样品载体止动组件具有至少一个样品载体;使至少一个抽屉具有至少一个样品载体止动组件;以及适当处理样品载体。 Another of the invention can achieve the aforementioned and other objects of the invention embodiments include: A sample processing method, the method comprising the steps of: a plurality of at least one close drawer (drawer); at least one sample carrier stopper component having at least one sample carrier; at least one drawer having at least one sample carrier stopper assembly; and appropriate treatment of the sample carrier. 适当处理步骤可自动处理样品并且可以连续或批处理样品,而且可以提供多个单独的载玻片处理,并且在一些实施例中可以提供丰余的载玻片处理,以便单独处理每个载玻片。 Appropriate processing step automatically processes the sample and may be continuous or batch samples, and can provide a plurality of individual slides treated, and in some embodiments may provide redundancy in the processing of slides, each slide glass so treated separately tablets.

本发明的实施例还包括一种自动样品处理方法,该方法包括以下步骤:收集方案信息;将方案信息传送给至少一个样品处理系统;适当处理样品;以及从适当处理步骤中收集样品处理信息。 Embodiments of the present invention further comprises an automatic sample processing method, the method comprising the steps of: collecting program information; transmitting scheme information to at least one sample processing system; appropriately treated sample; and collecting the sample processing information from the appropriate treatment step. 而且,实施例可提供:维护方案信息、维护样品处理信息、方案信息的信息共享以及样品处理信息。 Moreover, embodiments may provide: maintenance program information, maintaining the sample processing information, program information, and information sharing sample processing information. 这些或其它方法步骤可提供各个样品或多批处理、样品诊断特性、以及多批处理的实时或适应性能力。 These and other methods are available for each step or batch samples, sample diagnostic features, and more real-time or batch adaptive capacity.

本发明的许多方面可应用于免疫组织化学(IHC)技术,以及样品的原位杂交(ISH)和荧光原位杂交(FISH)特异性染色,还有微阵列,特别是包括样品的靶挽回(target retrieval)或染色的技术。 Many aspects of the present invention may be applied to immunohistochemistry (IHC) techniques, and in situ hybridization of the sample (ISH) and fluorescence in situ hybridization (FISH) specific staining, as well as microarrays, in particular a sample comprising a target restore ( target retrieval) or staining technique.

支持应该理解为是为本发明的以下方面和实施例而存在的:一种自动染色装置,包括:至少一个试剂容器;至少一个样品;适合影响所述试剂容器和所述样品的机器人部件;所述机器人部件响应的控制部件;以及为了对所述自动染色装置中的至少一个部件进行二维成像,而构造的图象捕获2-D光学传感器。 An automatic staining apparatus, comprising: a: The following aspects should be understood to support and embodiments of the present invention and the presence of at least one reagent container; at least one sample; robot member for the reagent container and the sample effects; the control means in response to said robot member; and for the automatic staining apparatus of at least one component in a two-dimensional image, and the image capture 2-D structure of the optical sensor.

一种识别自动染色装置中的至少一个特性的方法,包括以下步骤:提供至少一个样品; A method for automatic staining apparatus identifying at least one characteristic, comprising the steps of: providing at least one sample;

提供至少一个试剂容器;提供适合影响所述试剂容器和所述样品的机器人部件;光学探测所述自动染色装置中的至少一个部件的二维图象;记录相关图象数据;以及将所述图象数据馈送到所述机器人部件响应的控制部件。 Providing at least one reagent container; means for providing the robot affect the sample container and the reagent; optical detection of at least two-dimensional image of the automatic staining apparatus of one member; record relevant image data; and the FIG. image data to the control section of the robot part response.

一种在自动染色装置中染色样品的方法,包括以下步骤:提供至少一个样品;提供至少一个试剂容器;提供适合影响所述试剂容器和所述样品的机器人部件;提供响应所述机器人部件并适合探测所述自动染色装置中的至少一个部件的二维图象的光学传感器;记录相关图象数据;以及将所述图象数据馈送到所述机器人部件响应的控制部件。 A method for automatic staining apparatus stained sample, comprising the steps of: providing at least one sample; providing at least one reagent container; means for providing the robot affect the sample container and the reagent; provided responsive to said robot member and adapted detecting said automatic staining apparatus of the two-dimensional image of at least a part of an optical sensor; recording-related image data; and the image data will be fed to the control section of the robot part response.

一种自动染色装置,包括:至少一个试剂容器;至少一个样品;适合影响所述试剂容器和所述样品的机器人部件;所述机器人部件响应的控制部件;以及为了探测所述自动染色装置中的至少一个部件而构造的多功能光学传感器。 An automatic staining apparatus, comprising: at least one reagent container; at least one sample; adapted affect the reagent container and the sample components of the robot; robot part in response to said control means; and means for detecting in the Autostainer Multifunctional optical sensor at least one component constructed.

附图简述以下参照附图来描述本发明,其中:图1是按照本发明优选实施例的染色装置的示意性透视图;图2是图1所示染色装置中的工作区域的顶视图;图3是按照本发明一些实施例的染色装置中的机器人部件的细节图; BRIEF DESCRIPTION The following description of the present invention with reference to the accompanying drawings, in which: Figure 1 is a schematic perspective view of a dyeing apparatus according to a preferred embodiment of the present invention; FIG. 2 is a dyeing apparatus shown in Figure 1 a top view of the work area; Figure 3 is a detail view of the dyeing apparatus of the present invention, some embodiments of the components of the robot;

图4是具有光学识别装置的试剂瓶的顶视图;图5是其上具有光学识别标记的显微镜载玻片;图6是此标记布局的一个实例;以及图7-图10是载玻片上多种光学识别装置的实例。 Figure 4 is a top view of a reagent bottle having an optical recognition apparatus; FIG. 5 is on a microscope slide having an optical identification mark; Fig. 6 is an example of such a tag layout; and Figs. 7 to 10 on the glass slide is more Examples of types of optical recognition apparatus.

本发明的实施方式图1和图2中表示出按照本发明的自动染色装置1。 Embodiment of the present invention. FIG. 1 and FIG. 2 shows the present invention in accordance with the automatic dyeing apparatus 1. 自动染色装置1包括围绕试剂站或试剂部分2的矩形框架4,以及第一和第二载玻片部分5,试剂站或试剂部分2包括试剂瓶或容器室,其中每个室内放置有试剂瓶或试剂容器3,许多分离的轨道6放置在第一和第二部分5上,每个轨道6包括许多一个挨一个地安装在轨道6内的显微镜载玻片7。 Automatic staining apparatus 1 comprises a reagent station or around the rectangular frame portion 2 of the reagent 4, and first and second slide glass part 5, the reagent station comprises a reagent or reagents portion 2 bottle or container chamber, wherein the interior of each reagent bottle placed or the reagent container 3, a number of separate rail 6 is placed on the first and second portions 5, 6 of each track comprises a plurality of side by side rails 6 mounted within the microscope slide 7. 多个试剂容器、甚至载玻片以任何所需的顺序(例如阵列)来放置。 A plurality of reagent containers, or even the slides in any desired order (e.g., arrays) to be placed. 在图示的实施例中,每个轨道可容纳8个载玻片,但是轨道可设计成能够容纳任何适当数目的载玻片。 In the illustrated embodiment, each track can hold eight slides, but the track may be designed to accommodate any suitable number of slides. 由于一个挨一个地布置有8个轨道,因此图示实施例可容纳64个载玻片7,每个在载玻片的上面都固定有样品例如组织样品,因此试剂可从上方施加到每个载玻片上的样品中。 Since there are arranged side by side eight tracks, and therefore the illustrated embodiment can accommodate 64 slides 7, each of the above slides are fixed to a sample such as a tissue sample, thus the reagent can be applied from above to each of the sample on the slide. 所处理的样品可以是任何材料,但是最可能是生物材料例如生物样品或生物物种,也许是诸如组织样品例如组织或细胞物种、细胞、细胞集合或组织样品,定义包括细胞系、蛋白质及合成肽、组织、细胞前体、细胞制备品、血液、体液、骨髓、细胞学物种、血液涂片、薄层制备品和微阵列。 The treated sample may be any material, but the most likely biological material such as a biological sample or biological species, such as may be tissue samples such as tissue or cell species, cell, collection of cells or tissue samples, including cell lines definition, proteins and synthetic peptides , tissue, cell precursor, preparing goods cells, blood, body fluids, bone marrow, cytology species, blood smears, thin-layer preparation and microarray products. 还应该理解,包括载玻片基生物样品。 It should also be understood that the biological sample comprises a slide glass base.

如上所述,本发明可包括在某种程度上影响试剂容器和组织样品的机器人部件。 As described above, the present invention may include a robot part in some way affect the reagent container and tissue samples. 这样,对试剂容器或组织样品实施的任何类行为、由试剂容器或组织样品产生的行为或只是来自试剂容器或组织样品的信息,都可通过机器人部件容易地进行。 Thus, the behavior of any kind of reagent containers or tissue sample embodiment, the tissue sample from the reagent container or generated or simply acts or tissue sample from a reagent container information, can be easily carried out by a robot member. 机器人部件在实施例中,适合对施加有试剂等(包括作为结果或结合有)的载玻片进行染色。 In an embodiment, the robot parts, suitable for applying the reagent, etc. (including as a result of or in combination with) glass slides for staining. 机器人臂或机器人部件20还可将试剂从试剂容器转移到预定的组织样品上。 Robotic arm or robot part 20 can also be transferred from the reagent container reagent onto a predetermined tissue sample. 例如,用于沿X和Y(以及Z)方向(如箭头X和Y所示)移动探头10的机器人部件20,布置在染色装置的框架4上方。 For example, a robot member (as indicated by arrows X and Y shown) for moving the probe along the X and Y (and Z) direction 10 of 20, arranged in the frame 4 above the dyeing apparatus. 机器人臂可以将探头10定位在所有试剂瓶3以及所有载玻片7的上方,并且进一步操纵探头10,以吸取任一瓶3中包含的部分试剂,从而将那部分试剂传送并施加到任一载玻片7上,以便对每个载玻片7上的样品有选择地进行染色和处理。 Robotic arm 10 may be positioned on top of all the probe reagent bottle 3, and all the slides 7, and further manipulating the probe 10, in order to absorb any portion contained in the reagent bottle 3, so that part of the reagent will be applied to any transmission and slides 7, to selectively dyed and treated sample 7 on each slide. 控制部件通过控制染色过程的全部、甚至任何部分,来管理染色过程。 Control means by controlling the whole dyeing process, or even any part, to manage the dyeing process. 利用合适的控制部件或等同型控制装置(例如具有用于此目的的合适软件和输入数据的计算机(未示出)内的能力),该染色装置1能够对需要不同染色或处理试剂和过程的样品进行染色或处理。 Using suitable control means or equivalent type control means (e.g., having suitable software for this purpose and the input data of the computer (not shown capability) within), the dyeing apparatus 1 is capable of dyeing or treatment require different reagents and procedures The samples are dyed or treated.

如图1和3所示,探头10容纳在机器人头22中并由机器人部件20来操纵。 1 and 3, the probe 10 is accommodated in the robot head 22 in member 20 by a robot to manipulate. 探头10上升到上部位置(在Z方向),在此处清楚地看到,瓶3在探头10的下面,但是机器人可包括机器人头22中用于降低探头10的装置或部件,以便将探头尖浸到所选择的试剂瓶3的内容物中并吸取选定量的用于所选择的染色或处理过程的试剂。 Probe 10 rises to the upper position (in the Z direction), here clearly seen, the bottle 3 below probe 10, but the robot 22 may include a robot head 10 for lowering the probe apparatus or member, so that the probe tip immersed in the selected contents of the reagent bottle 3 and drawing a selected amount of reagent for the selected staining or treatment process. 在一个实施例中,本发明包括在机器人部件上配备光学传感器86,或许还包括通过机器人部件的动作将光学传感器移动到预定位置。 In one embodiment, the present invention includes an optical sensor 86 is equipped on the robot part, and possibly also by means of the operation of the robot will move the optical pickup to a predetermined position. 作为一个实例,机器人头22配有光学传感器86、甚至是指向下方的CCD照相机25。 As an example, the robot head 22 is equipped with an optical sensor 86, even below the CCD camera 25 point. 光学传感器位于机器人部件上、或许更广泛地响应机器人部件。 The optical sensor is located on the robot part, the robot may respond to a broader part. 在光学传感器定位之后,就在构建光学传感器的部位记录图象数据。 After the optical sensor positioning, image data is recorded in the construction site of the optical sensor.

在一些实施例中,机器人部件20、甚至机器人头22可包括各种元件,包括(但不限于):可与汽缸39相连的推动工具38、可响应甚至与弹簧泵37相连的探头移动部件36的探头10、以及光学传感器86(如图3所示)。 In some embodiments, the robot part 20, the robot head 22 may even include various elements, including (but not limited to): pushing tool 39 can be connected to the cylinder 38, can move in response to the probe even with the spring member 37 connected to the pump 36 The probe 10, and an optical sensor 86 (shown in Figure 3).

在实施例中,光学传感器可检测相关特性的二维图象数据。 In an embodiment, the optical sensor can detect two-dimensional image data-related characteristics. 它还适合在总体上探测部件的二维图象。 It is also suitable in general two-dimensional image detecting member. 利用照相机确定载玻片和试剂瓶以及装置在工作区域内的其它特性的状态信息,例如阅读配置在试剂容器上的代码,以确定系统内的试剂种类和试剂部位。 Using a camera to determine the slides and reagent bottles and means other characteristics in the work area of the status information, such as reading the reagent container arranged on the code to determine the reagents and reagent site within the system. 照相机还可确定组织样品载体的状态,例如特定玻片的部位、指示有关呈现在载玻片上的组织样品或待实施的处理方案的信息的信息索引例如代码。 The camera may also determine the status of a tissue sample carrier, such as a specific slide location, indicating the relevant tissue samples on slides presented the index information or the information processing scheme to be implemented, for example, code. 照相机可用于诊断目的。 The camera can be used for diagnostic purposes. 在一些实施例中,潜在地用计算机扫描样品,以便进一步进行分析。 In some embodiments, the computer scans the sample potentially for further analysis. 本发明在实施例中可包括计算机图象生物分析部件、或许甚至是用计算机对样品的图象数据进行生物分析。 In an embodiment of the present invention may include a computer image analysis of biological components, perhaps even with a computer for image data analysis of biological samples.

如上所述,本发明可包括记录各种相关图象数据。 As described above, the present invention may include a record of all relevant image data. 当然,可记录各种相关图象数据。 Of course, you can record a variety of related image data. 重要的是,这包括记录部件的校正参比点、或许甚至是装置上或装置内的机器人部件的校正参比位置。 Importantly, this includes a correction reference dot recording member, perhaps even correcting the reference position of the robot apparatus or the member within the device. 正如所提到的,本发明还可记录载玻片识别图象数据和试剂识别图象数据。 As mentioned, the present invention may also be recorded to identify the image data and the slide glass reagent identification image data. 一个实施例的显著方面是,可记录特定载玻片的光学识别部件、或许仅仅记录有关部件的信息。 A significant aspect of an embodiment is that the specific slides recordable optical recognition unit, perhaps only information about the recording member. 这样的信息当然包括有关组织样品的信息。 Of course, such information includes information about tissue samples. 同样,包括有关试剂容器内含有的试剂的信息的光学标识可记录在试剂容器上。 Similarly, the reagent container contents includes information about the optical identification information can be recorded in some of the reagent on the reagent containers. 它可将二维符号记录载玻片上、甚至试剂容器上。 It can record two-dimensional symbol on a glass slide, and even on the reagent container. 记录在载玻片上的二维符号所代表的数据包括(但不限于):试剂相关数据、试剂种类数据、试剂体积相关数据、试剂有效期相关数据等。 Recorded on the two-dimensional symbols represented by data of the slide glass including (but not limited to): a reagent related data, the reagent type data, data related to the reagent volume, the reagent is valid data and the like. 它还可提供连接部件,所捕获的图象数据通过连接部件传送给控制部件。 It also provides connection means, the captured image data to the control unit via the connecting member. 它包括将图象数据馈送到控制部件,以便响应机器人部件。 Which comprises feeding the image data to the control section, in response to components of the robot. 在记录相关图象数据之后、或许作为数据馈送到控制部件的结果,本发明可按照相关图象数据来操纵染色或其它过程。 After recording the image data is relevant, perhaps as a result of the data fed to the control member, in accordance with the present invention may be used to manipulate the image data related to the dyeing or other processes. 这样,本发明可按照组织特异性方案来进行载玻片的染色。 Thus, in accordance with the present invention may be tissue-specific programs for staining slides.

本发明的染色装置1还包括探头清洗站8和试剂混合器9,机器人部件20还布置成能够将探头运送到清洗站8以及试剂混合器9。 Dyeing device 1 of the invention further comprises a reagent probe wash station 8 and the mixer 9, the robot member 20 also arranged to be conveyed to the probe washing station 8 and a reagent mixer 9.

如图4所示,试剂瓶3在安装有光学识别部件的表面上配有区域30。 4, the reagent bottle 3 on the mounting surface of the optical member with a recognition area 30. 此光学标识可以是携带有关瓶3的内容物的信息(例如试剂种类、制造数据、有效期限等)的粘附标记31。 The optical marking may carry information about the contents of the bottle 3 (e.g., the reagent type, manufacturing data, expiration date, etc.) adhesion numeral 31. 所编码的信息是数据矩阵码形式、Infoglyph码形式或2-D码的任何其它类型,主要还是简单的1-D码即条形码。 The information is encoded in the form of a data matrix code, any other form or type of Infoglyph code 2-D codes, mainly simple 1-D code i.e. barcode. 此外,标记31还配有人可读的文本,以便诸如在将试剂瓶装载到染色装置内的过程中有助于操作者操纵试剂瓶。 Furthermore, marking 31 comes with a human-readable text, such as in the reagent bottle to be loaded into the process of staining within the device helps the operator to manipulate the reagent bottle.

图5表示出其上安装有标记71的载玻片7。 Figure 5 shows markers mounted thereon slides 71 7. 图6中表示出标记71的一个布局。 Figure 6 shows a layout of the mark 71. 标记71可以是粘附的光学标示,是为特定载玻片制备的并在标记印刷机(未示出)或任何其它合适的印刷设备上进行印刷。 Tag 71 may be adhered to the optical marking is produced in the tag for a particular slide and press (not shown) printed on, or any other suitable printing devices. 甚至在特定情形,如果一批载玻片接受相同的处理,则为这些载玻片提供一组相同的标记。 Even in certain instances, if the same number of slides accepted treatment, compared with a group of these slides provide the same reference numerals. 标记71包括用于有关载玻片7上组织样品的编码信息(例如病人数据、数据和文件号码、染色方案和/或处理步骤)的区域72。 Mark 71 includes coded information about the tissue samples (such as patient data, and the file number, staining solutions and / or processing steps) on 7 area for 72 slides. 而且,标记71配有一行或多行人可读的文本和/或空白空间,以便制备载玻片的实验室人员在载玻片标记上进行书写。 Moreover, marker 71 is equipped with one or more pedestrian-readable text and / or blank spaces, laboratory personnel to prepare slides on the slide that tag writing.

在图7-9中,表示出标记71的多种数据编码符号(如图所示的整个标记71,或者仅用于标记区域72(参见图6))。 In Figures 7-9, there is shown a plurality of data marks 71 coded symbols (as shown in the entire marker 71, or only for the mark region 72 (see FIG. 6)).

在图7中,表示出InfoglyphTM型的2-D符号。 In Figure 7, there is shown InfoglyphTM type of 2-D symbol. 这包括或许甚至是信息型地毯型(carpet)符号。 This includes information Carpet perhaps even type (carpet) symbol. 这种2-D符号是有益处的,因为它能够携带大量的光学机器可读信息。 This 2-D symbol is beneficial, because it can carry large amounts of optically machine readable information. 利用高分辨率照相机,这种类型的符号可以高分辨率来读取,并且大量信息编码在其中。 Using high-resolution cameras, high-resolution of this type of symbols may be read, and the large amount of information encoded therein. 将此符号用不同方向、或许甚至不同颜色的微小斜线来印刷,并且能够用CCD照相机等容易地读取。 This symbol in different directions, and perhaps even different colors to print small diagonal, and can be easily read by a CCD camera or the like.

图8表示出用作Infoglyph符号替换形式的数据矩阵码。 Figure 8 shows an alternative form of symbols used as Infoglyph data matrix codes. 数据矩阵同样用CCD照相机来读取,但是不可将编码中的许多数据作为Infoglyph来携带。 The same data matrix with a CCD camera to read, but not many of encoding data as Infoglyph to carry. 然而,如果载玻片和试剂瓶上需要的识别数据较少,则其就更容易印刷,因为它可具有不高的分辨率,从而是一种简单经济有效的解决方案。 However, if the reagent bottle on glass slides and identifying data needed less, then it is easier to print, because it does not have high resolution, and thus is a simple and cost-effective solution. 另一种更简单的解决方案在图9中示出,其中符号是老的条形码。 Another simpler solution is shown in Fig. 9, wherein the symbol is the old barcode. 在原理上这意味着,为了读取载玻片和试剂瓶的信息仅需要一个条形码扫描器,但是要使用2-D传感器,因此自校正和监测载玻片和试剂在染色装置中的安装的可能性得以增强。 In principle this means that, in order to read information slides and reagent bottle requires only a bar code scanner, but using 2-D sensor, thus monitoring and self-tuning slides and reagent installed in the dyeing apparatus possibility is enhanced.

在一个实施例中,载玻片上和试剂瓶上的光学标示是同一类型。 In one embodiment, the optical marking on glass slides and reagent bottles are of the same type. 这可容易地对染色装置中的识别过程进行图象处理。 This can be easily dyed device recognizing process for image processing.

一种识别各个载玻片的不同途径,或者是作为实施证实识别或储存证实信息的新能力的一种方式,可记录组织样品74本身的轮廓和/或纹理,例如如图10所示。 A different way of identifying individual slides, or as a way to identify or confirm the implementation of new authentication information storage capacity, a tissue sample can be recorded contour 74 itself and / or textures, such as shown in Figure 10. 通过采用照相机能够记录的图象的高分辨率,组织样品本身的独特性质可用作载玻片的图表标示。 By using high-resolution camera capable of recording an image, the unique nature of the tissue sample itself may be used as a slide glass graph marked. 而且,能够记录已染色的组织样品的图象,从而产生组织样品的数字表示形式。 Moreover, the ability to record images of the tissue sample has been stained, thereby generating a digital representation of the tissue sample. 该数字图象通过电子传送到用于立即检查的远程地方和/或为随后检查存档。 The digital image is transmitted to a remote place to check for immediate and / or archived electronically for subsequent examination. 这可使染色装置在使用上具有独特的灵活性,且可以引入新颖有益的组织样品分析方法。 This allows staining apparatus having a unique flexibility of use, and may introduce a novel method useful for tissue sample analysis.

除了识别染色装置中的显微镜玻片和试剂瓶之外,2-D光学传感器还能够用于装置的自校正,诸如在装置维修之后,如果装置已经拆卸并运送到另一个地方的话。 In addition to identifying the dyeing apparatus microscope slide and reagent bottles, 2-D optical sensors can also be used for self-correcting device, such as after the device repair, if the device has been removed and transported to another place of the words. 通过用照相机捕获图象来识别装置内部的临界部位,图象处理软件可以将所捕获的图象与参比图象进行比较,以确定装置内的某些临界元件是否偏离它们的预定位置,诸如滑轨或玻片是否稍稍偏离,如果偏离,就计算机器人运动控制系统的一组校正数据,并将这组数据用于校正装置。 The captured image by the camera to identify the critical parts inside the device, image processing software can be captured image is compared with the reference image to determine certain critical element within the apparatus has deviated from their predetermined positions, such as or is slightly deviated from the slide rail, if deviation correction data to calculate a set of robot motion control system, and means for correcting the set of data. 如果所需的校正超过某一尺寸,就向操作者自动发出警告,从而确保装置在载玻片处理过程中不发生故障。 If the desired correction exceeds a certain size, it sends an alarm to the operator, thereby ensuring that no failure occurs in the device processing slides. 而且,此图象分析系统还用于确定载玻片是存在于轨道上还是脱离轨道,以产生警告信号。 Moreover, the image analysis system is also used to determine the slides are present on the track or off track to generate a warning signal.

通过本发明,应该意识到,在不脱离如权利要求书限定的本发明范围的前提下,可以对本发明一些优选实施例的上述描述作出各种改变。 By the present invention, it should be appreciated that in the appended claims without departing from the scope of the invention as defined in the premise, the present invention can be of some preferred embodiment of the above described embodiments and that various changes. 正如所容易理解的,本发明的基本概念可以各种方式来实施。 As readily understood, the basic concepts of the present invention can be implemented in various ways. 它包括染色技术以及用于完成染色和其它功能的多种系统、组件和设备。 It includes staining techniques and a variety of systems, components, and equipment used to complete the dyeing and other functions. 在该申请中,染色技术也是作为用所述的多种系统、组件和设备来完成的所示部分结果以及作为固有采用的步骤来公开的。 In this application, the staining technique is shown in part as a result of a variety of systems, components, and equipment used to complete the well as the steps inherent to the use of disclosed. 它们应该被理解为,采用预计和描述的设备的自然结果。 They should be understood as natural results are expected and apparatus described herein. 此外,虽然公开了一些设备,但是应该理解,这些不仅完成了一些方法,而且还以许多方式来变化。 Further, although some devices are disclosed, it should be understood that these not only completed a number of methods, but also be varied in many ways. 重要的是,对于上述所有内容,所有这些方面都应该理解为包括在本发明的公开文本中。 Importantly, for all of the above, all of which should be understood to include disclosure of the present invention.

读者应该意识到,具体论述不可明白地描述所有可能的实施例;许多变型都是含蓄的。 The reader should be aware that the specific discussion may not explicitly describe all embodiments; many variations are subtle. 它还不能充分解释本发明的总的性质,不能明白地表示每一特性或部件是如何实际代表更广泛功能或各种替换型或等同部件的。 It can not fully explain the general nature of the invention, not showing clear how each feature or component actually represent a wider variety of functions or replacement-type or equivalent member. 再者,这些都明显包括在此公开文本中。 Furthermore, these are obviously included in this disclosure text. 当本发明用偏向设备的术语来描述时,设备的每个部件都明显行使一个功能。 When the deflection device of the present invention, the term used to describe, for each part of the apparatus are significantly perform a function. 对于所述设备不仅包括装置权利要求,而且为了实现本发明和每个部件实施的功能,还包括方法或工艺权利要求。 The apparatus comprises a means for not only the claim, and in order to implement the present invention and the functions implemented in each member, further comprises a method or process claims. 无论是说明书还是术语都无意限定本公开文本的范围。 Whether manual or terms are not intended to limit the scope of the present disclosure.

还应该理解,在不脱离本发明本意的前提下可以做出各种变化。 It should also be understood that the intention of the present invention without departing from the premise that various changes may be made. 这些变化也都明显包括在本说明书中。 These changes are also included in the present specification clearly. 它们还落在本发明的范围内。 They also fall within the scope of the invention. 包括图示的示范性实施例、各种含蓄替换型实施例以及广泛的方法或工艺等的泛泛公开内容都包含在本公开文本中,并且据此用以支持本专利申请或随后专利申请的附加权利要求。 Including the exemplary embodiments illustrated, alternative embodiment for various subtle and extensive methods or processes such as general disclosures are included in this disclosure, and accordingly to support this patent application or subsequent additional patent applications claims.

而且,本发明及权利要求的每个部件还可用各种方式来实现。 Moreover, each component of the invention and claimed in the present claim may be implemented in various ways. 本公开文本应理解为包括每个这样的变型,即任何装置实施例、方法或工艺实施例、或者仅仅是其中任何部件的变型。 This disclosure should be understood to include each of such variations, i.e. cases, a method or process embodiment any apparatus embodiment, wherein the variant or simply any component. 尤其是,应该理解为,当本公开文本涉及本发明的部件时,每个部件的词汇可以由等同的装置词汇或方法词汇来表达,即使仅仅功能或结果是一样的。 In particular, it should be understood that when the present disclosure relates to a component of the present invention, each word component can be expressed by equivalent apparatus or method vocabulary words, even if only the function or result is the same. 这样的等同、延伸、甚至更多派生词应该认为是包括在每个部件或行为的描述中。 Such equivalents, extending, or more derivatives should be considered to be included in the description of each part or behavior of. 当期望解释本发明所涉及的广泛范围时,这样的词汇是可替代的,其中需要解释授予本发明的隐含宽泛范围。 As widely expected to explain the scope of the invention when such words are fungible, which implied the need to explain the broad scope of the present invention is granted. 但是作为实例,应该理解,所有的行为都可表达为实施该行为的方式或产生该行为的部件。 As an example, however, it should be understood that all actions can be expressed as the embodiment of the generating means the act or acts. 同样,所公开的每个物理部件应该理解为包括物理部件所实施的行为的公开内容。 Similarly, each physical member disclosed should be understood to include the behavior of the physical components of the embodiment of the disclosure. 关于最后这方面,作为一个实例,“传感器”的公开应该理解为包括“探测”行为的公开,而无论是否是明显论述的,反之,如果有“探测”行为的有效公开,这样的公开就应该理解为包括“传感器”、甚至“探测方式”的公开。 Regard to this last aspect, as an example, "sensor" should be understood to include disclosure "probe" behavior disclosed, regardless of whether it is obvious discussed, on the contrary, if there is "detected" acts effectively disclosed, such disclosure should understood to include the "sensor" and even "probe mode" in public. 还应该理解,在具体语言可直译为限定作用的权限中,作为美国的一个实例,其中对部件“装置(means for)”的一些解释被限制窄了,而更宽泛的等同语言(例如“部件”等)可用来避免窄的解释,并且应该理解为被本说明书所包含。 It should also be understood that, in a particular language can be literally translated as the permissions defined role, as one example of the United States, of which some explanation of parts "means (means for)" is limited to narrow, and broader equivalent language (e.g., "parts "etc.) can be used to avoid a narrow interpretation, and should be understood to be included in the present specification. 这样的变化和等同词汇应该理解为明显包括在此描述中。 Such variations and equivalents should be understood as distinct words included in this description.

本专利申请中提到的任何专利、专利申请、公开出版物或其它参考文献在此处都作为参考并入本文。 Any patents, patent applications, publications, or other references mentioned in this patent application are herein incorporated herein by reference. 此外,对于所用的每个词汇,应该理解,除非其在本申请中的使用与这些解释不符,否则通用的词典定义都应该理解为是针对每个词汇的,并且诸如the Random House Webster's Unabridged Dictionary(第二版)中包含的所有定义、等同词汇和同义词,在此都作为参考并入本文。 In addition, for each vocabulary used, it should be understood that, unless their use in this application are inconsistent with these explanations, otherwise the common dictionary definitions should be understood to be for each vocabulary, and such as the Random House Webster's Unabridged Dictionary ( Second Edition All definitions) contained, equivalent words and synonyms herein are incorporated by reference herein. 最后,任何本申请的以前案件都附属在此并且作为参考并入本文。 Finally, in any case before the present application and are affiliated herein incorporated by reference herein.

这样,这些申请应该理解为对至少以下权利要求是支持的:i)正如本文所公开和描述的,每个样品处理系统和子系统,ii)所公开和描述的相关方法,iii)每个这些系统、组件、设备和方法的类似、等同、甚至是隐含的变型,iv)正如所公开和描述的,完成所示每个功能的那些替换型设计,v)完成所示每个功能的那些替换型设计和方法,vi)作为单独和独立发明示出的每个特征、元件和步骤,vii)由所公开的多种系统或元件增强的应用,viii)由这样的系统或元件产生的最终产品,以及ix基本上作为此前描述的以及参照任何附带实例的方法和系统、组件、设备及装置,x)所公开的每个部件的多种组合及变换,vi)正如附加在所表现的每个独立权利要求和概念上的,每个潜在的从属权利要求,xii)如上所述的,借助于计算机实施的方法,xiii)如上所述的,可编程的系统,xiv)如上所述的,用数据编码的计算机可读存储器,所述数据用于指导包括行使功能的装置或部件的计算机,xv)象本文所公开和描述的那样构造的计算机,xvi)如本文所公开和描述的,单个或组合压规程和程序,xvii)所公开和描述的相关的方法,xviii)这些每个系统和方法的相似、等同、甚至是明显的变型,xix)正如所公开和描述的,完成所示每个功能的那些替换型设计,xx)完成所示每个功能的那些替换型设计和方法,xxi)作为单独和独立发明示出的每个特征、元件和步骤,xxii)以上每个发明的多种组合及变换。 Thus, these should be understood to apply to at least the following claims are supported: i) as herein disclosed and described herein, each sample handling systems and subsystems, ii) the related methods disclosed and described, iii) each of these systems Those alternatives, similar components, devices and methods, equivalent, and even implicit variations, iv) As disclosed and described herein, complete replacement of those shown in each function design, v) to complete each function as shown in design and methods, vi) as a separate and independent invention shows each of the features, elements or steps, vii) final products from a variety of systems or components to enhance the application of the disclosed, viii) by such systems or components produced , and various combination and transformation ix substantially any accompanying reference examples as well as methods and systems, components, devices and apparatus described hereinbefore, x) of each component disclosed, vi) As demonstrated in each of the additional independent claims and conceptual, each potential dependent claims, xii) described above, by means of a computer-implemented method, xiii) described above, a programmable system, xiv) described above, with a computer readable memory encoded data, the data used to guide the exercise means comprising a computer or member functions, xv) as disclosed herein and computer constructed as described, xvi) as herein disclosed and described, single or COMPOSITE rules and procedures, xvii) related methods disclosed and described, xviii) similar to each of these systems and methods, equivalent, or even obvious variant, xix) as disclosed and described, as done in each Replace those design features, xx) who completed the replacement design and methods shown in each function, xxi) as separate and independent of each issued a clarification of the features, elements and steps, xxii) a variety of each of the above invention combination and transformation.

另外,如果使用或在使用时,按照传统的权利要求解释,过渡句“包括”等的使用,在本文是用来维持“开放式”权利要求的。 Also, if you use or is in use, according to the traditional interpretation of claims, the transition period "includes" and used in this paper is used to maintain the "open" claims. 这样,除非上下文有其它要求,否则应该理解,词汇“包括(comprise)”或诸如“包括(comprises)”或“包括(comprising)”等的变型,都意指暗示包括规定部件或步骤或部件或步骤的组合,但不排除任何其它部件或步骤或部件或步骤的组合。 Thus, unless the context other requirements, otherwise it should be understood that the word "include (comprise)" or the like "includes (comprises)" or "including (comprising)" and other variations, are meant imply the inclusion of a predetermined part or parts, or steps or combinations of steps, but not the exclusion of any other combination of elements or steps or components or steps. 这样的词汇用其最广泛的形式来解释,以便为本申请提供最宽泛的法律许可的范围。 Such vocabulary used in its broadest form to explain to the present application provides the broadest extent permitted by law.

所提出的任何权利要求必然作为本发明描述的一部分以参考文献的形式并入本发明,并且申请人清楚地保留用这些权利要求的所有或一部分组合内容作为额外说明书描述的权利,以便支持任何或全部权利要求或其任何部件或元件,申请人还清楚地保留将这些权利要求的任何部分或全部组合内容或其任何部件或元件从说明书的描述中移到权利要求内的权利,或者反之亦然,以便规定本申请或其任何随后继续、分立或连续申请所寻求的保护主题,或者以便在追踪或符合任何国家或条约的专利法、规则或规章的费用方面得到减少的好处,并且以参考文献的形式并入的这些内容应该在本申请的整个待审过程中有效,包括其任何随后继续、分立或连续的申请或任何重新发行物或扩展物。 Any rights that the claim is bound as part of the present invention is described as incorporated herein by reference, and is claimed by the applicant is clearly a combination of all or part of the content of these claims as additional description describes the retention, in order to support any or all the claims or any parts or elements, the applicant also clearly retain all combinations of any part of these claims or the contents or any member or element as claimed in claim moved from within the description of the specification, or vice versa to the regulations or any benefit then continues, discrete or continuous application sought protection theme, or to be reduced in line with the cost of tracking or any country or patent law treaty, rule or regulation of the aspects of the present application, and by reference The form of these elements are incorporated in the present application should be valid throughout the pending process, including any subsequently continued, discrete or continuous application thereof or any reissue or extension thereof.

Referenced by
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CN102565428A *5 Aug 201111 Jul 2012美国樱花检验仪器株式会社Automated system and method of processing biological specimens
CN102565428B *5 Aug 201116 Dec 2015美国樱花检验仪器株式会社处理生物标本的自动化系统和方法
CN102854050A *29 Sep 20122 Jan 2013梁建中Fully-automatic double-rail staining instrument for frozen sections
CN104053979A *15 Nov 201217 Sep 2014莱卡生物系统墨尔本私人有限公司Biological sample treatment apparatus
CN104101529A *27 Jul 201415 Oct 2014李强Pathological dyeing device
CN104101529B *27 Jul 201420 Jul 2016李强病理染色装置
CN104630055A *8 Nov 201320 May 2015西安科技大学Main control board of in-situ hybridization system
CN104777027A *25 Mar 201515 Jul 2015江苏硕世生物科技有限公司Multifunctional fully-automatic Gram staining instrument
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