CN1448723A - Biological chip capable of being disassembled and assembled - Google Patents
Biological chip capable of being disassembled and assembled Download PDFInfo
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- CN1448723A CN1448723A CN 02113540 CN02113540A CN1448723A CN 1448723 A CN1448723 A CN 1448723A CN 02113540 CN02113540 CN 02113540 CN 02113540 A CN02113540 A CN 02113540A CN 1448723 A CN1448723 A CN 1448723A
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- probe card
- orifice plate
- probe
- reaction tank
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Abstract
The detachable biochip with one solid carrier and capable of being used in analyzing several specimens consists of base plate, probe plate on the base plate, perforated plate on the probe plate with holes corresponding to the array probes, bolt mechanism for positioning the probe array, and irreversible sealing mechanism to form sealing between the probe plate and the perforated plate. The probe plate includes several probe arrays, each of which may be used in several analyses. Before scanning, the sealing mechanism makes the liquid medium in different zones maintained separately, and during scanning, the sealing mechanism is detached for near distance scanning. The present invention has enlarged analysis range, high analysis speed and relatively low biochip analysis cost.
Description
Technical field
The present invention relates to a kind of biochip, particularly a kind of many array bio-chips.
Background technology
Biochip, be meant that the microarray that contains the biologically active material that is coated on the solid phase carrier (comprises DNA, antigen, antibody, cell, biologically active microarrays such as medicine), and can hatch it, washing waits compatible reaction and purification process, a kind of analytic product that can discern label by devices such as scanners.According to the difference of the probe array number of the affine material that is coated on the biologically active on the solid phase material, biochip can be divided into single array chip and many array chips.Single array chip can only be analyzed one fen sample in design, and many array chips can divide sample analysis to the M more than a minute in design.According to the difference of chip surface structure, biochip has planar chip and concave surface chip.At present, planar chip only is used for single array chip, and the concave surface chip is mainly used in many array chips.Planar chip only has a plane, and probe array region and edge surface thereof are in same plane.Its production method is, directly bioprobe is fixed on this surface plate to form probe array, and need not to remake forming processes can use.The concave surface chip has two above planes, and its probe array region is lower than this regional boundary surface, thereby forms a reaction tank.All application of samples are hatched, and operations such as washing are all carried out in reaction tank.The production method of existing concave surface biochip has two kinds.First method is a stationary probe after the first moulding, and promptly by mold pressing, technologies such as grinding out form concave surface on chip material, bioprobe is fixed in to form an independently probe array on each in type concave surface then.Second method is first stationary probe aftershaping, is about to bioprobe and at first is fixed in dull and stereotyped going up and forms M probe array, and then make a compound concave surface with technology such as bonding or chimeric, to make reaction tank usefulness.The production method of existing concave surface chip can only be produced the chip with permanent concave surface.The advantage of planar chip is the restriction of scanner few, can carry out the scanning of near-zero distance to label, and shortcoming is to have only a probe array, can only handle a sample.Though and present concave surface chip can wrap by a plurality of probe arrays and each probe array and all is in the matrix reaction tank, be easy to application of sample, hatch, washing waits the operation of reaction, but shortcoming is when scanning, owing to have the interface of permanent reaction tank height and protrusion, than planar chip to scanner more specific (special) requirements to be arranged, thereby limited its application widely.
Summary of the invention
Purpose of the present invention is intended to overcome the shortcoming of above-mentioned existing chip, has concave structure for the user provides a kind of when reacting, and have the biochip of the detachable use of planar structure when scanning.This chip is when finishing the sufficiently high reaction tank height of reaction needed, with two-layer or two-layer above material, through non-irreversible being sealed to form of surface of contact; Scanning when not needing the reaction tank height, only needing to contain the probe card and other material that the reaction tank height is provided of probe array, separating with physics mode and get final product.
The object of the present invention is achieved like this:
A kind of biochip of detachable use is characterized in that and can install and remove in use as required, when promptly each probe array is isolated mutually on the same chip of needs, is assembled into the structure that can isolate mutually, when not needing to isolate isolation structure is removed.The core of this chip is a flat board (probe card) that is fixed with M probe array, the basic structure of this chip is by probe card, base plate, spacer sleeve (when needing), become orifice plate, register pin and non-irreversible sealing mechanism are formed (basic structure as shown in Figure 1); Place the probe card that outside surface is printed with M probe array on the base plate, be placed to orifice plate on the plate face of probe card, become be equipped with on the orifice plate with probe card on M the corresponding reaction tank of giving of probe array with M eyelet highly, (in needs, also can M disposable spacer sleeve of isolating open column will be contained, embed in M the eyelet of orifice plate, with the reactant liquor phase medium with become the orifice plate isolated), be placed on the one-tenth orifice plate of probe card, by be arranged on its plate face with base plate on dowel hole is connected with pin that pin is formed entire chip is located, make probe card and become between the orifice plate or probe card by non-irreversible sealing mechanism, barrier film, become the surface of contact of (under the situation of using barrier film) between the orifice plate to form not leakage sealed;
The biochip of this detachable use, its production method also differs widely with the production method of present biochip: 1) it can independently be produced by sub-unit, only recombinant is installed in use, 2) can directly bioprobe be fixed on M array of formation on the planar substrates, no longer carry out the PROCESS FOR TREATMENT that other forms permanent concaveconvex structure, can directly use 3 with other parts) except that probe card, other parts of being produced can be disposable, also can use repeatedly.
Probe card among the present invention, it is characterized by: the individual independently probe array of M (M 〉=1) is arranged on the flat board, probe array region and border thereof (discrepancy in elevation is less than 0.2mm) all in the same plane, each probe array in use can be handled a sample.The material of this probe card can be a glass, and plastics, film and other can be used for the material that bio-chip substrate is made.The production of middle probe plate of the present invention, can distinguish fully with the production of present biochip and to come: because it can be fixed a plurality of probe arrays and produce difference with existing planar chip and come on a plate, because it is not have other production technology that generates concave surface processing after fixing a plurality of probe arrays on the flat board again, just can directly supplies with the user and come with existing concave surface chip difference.
One-tenth orifice plate among the present invention, it is characterized by: have on the plate with above-mentioned probe card on M M the hole that probe array place regional area is corresponding, there are enough height in these holes, to guarantee that M the reaction tank that itself and above-mentioned probe card zone forms has enough height, can guarantee application of sample, hatch, cleaning etc. with test relevant being reflected at and independently carry out in each reaction tank; This one-tenth orifice plate can be a kind of material, as iron content or nonferrous plastics, and glass, pottery, stainless steel also can be to be composited by these materials, for example adds one deck rubber diaphragm in stainless bottom surface to increase sealing property and protection probe card.It can be disposable becoming orifice plate, also can be nonexpondable.Its production can be carried out with probe card is supporting, also can independently carry out, and can also produce as parts of bio chip device.
Spacer sleeve of the present invention, it is characterized by: the cylindrical sleeve that contains hollow in the chamber that M makes by rubber or plastics or rubber/plastic composite (for example the plastic hollow post adds the rubber blanket film), place them on the probe card in the need time spent, respectively each probe array ecological segregation is sealed and embeds in the eyelet of orifice plate, in order to reactant liquor is kept apart with becoming orifice plate, guarantee into that orifice plate in use can not pollute and reusable.Spacer sleeve of the present invention is disposable use parts.Its production can be carried out with probe card is supporting, also can independently carry out, and can also produce as parts of bio chip device.
Non-irreversible sealing mechanism among the present invention, it is characterized by: 1) probe card with become all surface of contact between the orifice plate (when not using spacer sleeve only for becoming surface of contact of orifice plate/probe card, orifice plate/spacer sleeve/two surface of contact of probe card are arranged into when using spacer sleeve) upward formation sealing, avoid reaction medium to see through this surface of contact and leak, the pollution between reaction tank occurs; 2) above-mentioned sealing right and wrong are irreversible, and promptly when the needs reaction tank had enough height, above-mentioned surface of contact must be in sealing state, and when not needing the reaction tank height, above-mentioned surface of contact can separate.The concrete form of this sealing mechanism includes: 1. become orifice plate enough closely to be pressed together under the self gravitation effect on the probe card, form M independent mutual impervious reaction tank; 2. become rubber concave surface on the orifice plate under vacuum action closely attached to probe card on, form M independent mutual impervious reaction tank; 3. become orifice plate under the effect of screw-driving power, closely to be pressed together on the probe card, form M independent mutual impervious reaction tank; 4. become orifice plate outside boundary's pressure act on down and closely be pressed together on the probe card, form M independent mutual impervious reaction tank; 5. become orifice plate under the clamping force effect between dop and the draw-in groove, closely to be fastened on the probe card, form M independent mutual impervious reaction tank; 6. become orifice plate under magnet (permanent magnet or electromagnet) magneticaction, closely to be pressed together on and form M independent mutual impervious reaction tank on the probe card.
Base plate among the present invention; be parts of above-mentioned non-irreversible sealing mechanism and alignment device; it is characterized by: obligato part and be fixed with alignment device in the irreversible sealing mechanism of right and wrong; its manufacturing materials can be iron content or nonferrous plastics, rubber, pottery; glass; steel, material and their compounds such as magnet, for example the attached one deck rubber of steel surface with the protection probe card.Its production can be carried out with probe card is supporting, also can independently carry out, and can also produce as parts of bio chip device.
The invention has the advantages that: with minimum production cost, realized that biochip (comprises application of sample when reaction, hatch, operations such as washing) have than high-freedom degree reaction conditions (for example: the reaction tank height, volume etc.), (for example: entire chip is in same plane during in scanning the time to have the condition of scanning than high-freedom degree in scanning, the height of entire chip can minimize), thereby realized production than the many array bio-chips of high-freedom degree with minimum cost, for on same probe card, carrying out the multiple analysis of various product, provide an economically viable scheme.
Description of drawings
Fig. 1 is a chip structure vertical profile synoptic diagram of the present invention
Fig. 2 is a probe card synoptic diagram of the present invention
Fig. 3 relies on into the chip structure vertical profile synoptic diagram that orifice plate gravity is realized non-irreversible sealing example for the present invention
Fig. 4 relies on into orifice plate for the present invention and probe card surface of contact place vacuumizes the chip structure vertical profile synoptic diagram of realizing non-irreversible sealing example
Fig. 5 cuts open chip structure vertical profile synoptic diagram for what the present invention relied on that the power of being threaded realizes non-irreversible sealing example
Extraneous mechanical pressure realizes the chip structure vertical profile synoptic diagram of non-irreversible sealing example to Fig. 6 for the present invention relies on
Fig. 7 connects the chip structure vertical profile synoptic diagram of realizing non-irreversible sealing example for the present invention relies on cassette
Fig. 8 realizes the chip structure vertical profile synoptic diagram of non-irreversible sealing example for the present invention relies on attraction of permanent magnet
Fig. 9 realizes the chip structure vertical profile synoptic diagram of non-irreversible sealing example for the present invention relies on electromagnet suction
Mark among the figure: 1 for becoming orifice plate, and 2 is spacer sleeve, and 3 is probe card, 4 is base plate, and 5 is vacuum-pumping pipeline, and 6 is threaded hole, 7 is screw, and 8 is external force, and 9 is dop, 10 is draw-in groove, and 11 is permanent magnet, and 12 is electromagnet, 13 is register pin, and 14 is dowel hole, and 15 for becoming orifice plate self counterweight, 16 are into the eyelet on the orifice plate, and 17 is the probe card mounting groove.
Embodiment
Below in conjunction with the embodiment accompanying drawing, the present invention is done further detail explanation:
The basic structure of the biochip of the detachable use of the present invention as shown in Figure 1, it is by becoming orifice plate 1, probe card 3, base plate 4, register pin 13 is formed; Probe card 3 is positioned in the groove 17 of base plate 4, and spacer sleeve 2 embeds in the eyelet 16 of orifice plate 1 when need are isolated, and is becoming between orifice plate 1 and the probe card 3, and register pin 13 is located whole biochip.Realize sealing function under physical action, (having under the situation of spacer sleeve, between the post hole and probe card 3 for spacer sleeve) forms the reaction tank of M sealing between eyelet on the one-tenth orifice plate 1 and the probe card 3.
Example 1: the production of many array probes plate
Utilize present some model machine (this experiment uses U.S. Microarray printer XMM47.832 type manually to put model machine), hepatitis C antigen (BJ Children's Hospital's hepatopathy research institute), the third liver peptide antigen P21 (French SEDAC company), the third liver NS4 district peptide antigen (French SEDAC company) and the third liver NS3 district proteantigen (BJ Children's Hospital's hepatopathy research institute), totally four kinds of probes, concentration with 1mg/me, (wide * long * thick) is of a size of on the slide of having made activation processing of 25 * 75 * 1mm (French SEDAC) o'clock to one, its point sample mode is: each antigen has four probes, 16 probes of four kinds of antigens form one 4 * 4 array, each probe array is of a size of 4 * 4mm, 14 probe arrays of 2 row are arranged on each slide, whenever show 7.With the confining liquid sealing, clean then behind the point sample, dry up, promptly make 4 antigen probe plates (Fig. 2) of 14 standby arrays.
Example 2: the example (Fig. 3) that carries out non-irreversible sealing by gravity
In the present invention, become orifice plate 1 under the action of gravity of self, closely be pressed on the probe card 3, rely on into the gravity of orifice plate 1 self, cooperate the effect of spacer sleeve 2, be embodied as and form M reaction tank that seals between orifice plate 1 and the probe card 3.When not needing reaction tank after drying up, only needing has the gravity of spacer sleeve to become orifice plate to remove embedding, just can obtain the multiprobe array planar chip for scanning.
Example 3: by vacuumizing the example of realizing non-irreversible sealing
As shown in Figure 4, becoming on the orifice plate 1 has vacuum line 5, and when the one-tenth orifice plate 1 of having adorned spacer sleeve 2 was pressed on the probe card 3, pipeline 5 outwards vacuumized by finding time, under the atmospheric pressure effect, will become orifice plate 1, spacer sleeve 2, probe card 3 threes closely to force together, form the reaction tank of M sealing.When not needing reaction tank, only need open vacuum-pumping pipeline and make it fill air with, can remove spacer sleeve and become orifice plate, only use probe card.
Example 4: the example of realizing non-irreversible sealing by helicitic texture
As shown in Figure 5, become on orifice plate 1 and the base plate 4 corresponding threaded hole 6 is arranged, use screw 7 will become orifice plate 1, spacer sleeve 2, probe card 3, base plate 4 closely to combine, form the reaction tank of M sealing, when not needing reaction tank, only need turn on spiral shell and make a call to 7, can remove into the orifice plate spacer sleeve, continue to employ probe card.
Example 5: the example of realizing non-irreversible sealing by exterior mechanical gravity
As shown in Figure 6, become orifice plate 1, spacer sleeve 2, probe card 3, base plate 4 down, closely press together, form the reaction tank of M sealing in external force 8 (as impressed pressure, pincers power etc.) effect.When not needing reaction tank, only need remove external force screw thread is reversed, can unload into orifice plate and spacer sleeve, take out probe card.
Example 6: connect the example of realizing non-irreversible sealing by cassette
As shown in Figure 7, become on the orifice plate 1 two dops 9 are arranged, two draw-in grooves 10 are arranged on the base plate 4, probe card 3 is placed on the base plate 4, and after the one-tenth orifice plate 1 that will adorn spacer sleeve 2 is placed on the probe card 3, two dops 9 are pressed in the draw-in groove 10, are embodied as between orifice plate 1, spacer sleeve 2, probe card 3, the base plate 4 and combine closely, form the reaction tank of M sealing.When not needing reaction tank, only need press down dop 9 releasings and connect, can unload into orifice plate and spacer sleeve, take out probe card with the buckle of 10 of draw-in grooves.
Example 7: the example of realizing non-irreversible sealing by permanent magnet
As shown in Figure 8, permanent magnet 11 is housed in base plate 4, the material that becomes orifice plate 1 is the stainless steel compound substance of stainless steel and one deck rubber (when not the using spacer sleeve as), when with probe card 3, spacer sleeve 2, when becoming orifice plate 1 to be successively placed on the base plate 4,11 pairs of steel of magnet become orifice plate 1 that bigger suction is arranged, combine closely being embodied as under this suction between orifice plate 1, spacer sleeve 2, probe card 3, the base plate 4, form the reaction tank of M sealing.
Example 8: by the example of the non-irreversible sealing of high-speed double electromagnet
As shown in Figure 9, electromagnet 12 is housed in base plate 4, the material that becomes orifice plate 1 is the stainless steel compound substance of stainless steel and one deck rubber (when not the using spacer sleeve as), probe card 3, spacer sleeve 2, one-tenth orifice plate 1 are successively placed on the base plate 4, when electromagnet 12 energisings, become orifice plate 1 that bigger suction is arranged to steel, combine closely being embodied as under this suction between orifice plate 1, spacer sleeve 2, probe card 3, the base plate 4, form the reaction tank of M sealing.
Example 9: reaction and the example that scans
The probe card of making by example 1, then by example 2 to 7 different device chips that example 8 obtains, pass through compatible reaction in this example, behind the purification reaction, take the probe card that barrier assembly will have label apart and scan.Its process is just the same:
From the third liver standard items (deciding institute), select 3 negative samples (numbering 1 available from Chinese biological goods and medicine inspection
-, 2
-, 21
-) and 9 positive ( numberings 1
+, 4
+, 7
+, 21
+, 24
+, 31
+, 35
+, 38
+, 39
+), with confining liquid these samples are done 20 times of dilutions, respectively get then in the reaction tank that the 50ul dilute sample is added to these 7 chips respectively.Hatched 1 hour at 37 ℃, more respectively with 50ul cleansing solution washing four times.Get 50ul more respectively and add in the reaction tank of chip, hatched 1 hour under 37 ℃ with the rhodamine mark IgG-A-M of confining liquid dilution.After hatching out, to each reaction tank washing four times, distilled water washs secondary with cleansing solution, and absolute ethyl alcohol is developed a film once, and room temperature is dried.So far reaction finishes, and different according to chip then non-irreversible sealing mechanisms splits out isolation sealing component and takes out probe card, puts into scanner respectively and carries out the scanner uni data processing, and the gained result is as follows:
Table 1 (example 4 obtains): the result of biochip in example 8 tests
| Blood serum sample | RIBA result | The analyzing biochips result | |||
| Anti-HCV | Core | NS3 | NS4 | ||
| 1 -2 -21 -1 +4 +7 +21 +24 +31 +35 +38 +39 + | ? ? ? NS3 +NS3 ++NS3 ++++NS3 ++++NS3 ++++NS4 +NS3 ++NS4 +NS3 ++++NS3 +Core ++ | 545(45) 657(63) 589(59) 13254(768) 7453(879) 12432(650) 28547(3965) 21784(2509) 124611(973) 25423(1795) 10568(798) 39034(2543) | ? ? ? 325(59) 469(56) 342(54) 356(41) 395(42) 387(54) 476(67) 524(45) 43985(3098) | ? ? ? 352(49) 5498(420) 4563(654) 13568(2904) 481(65) 5248(657) 6830(597) 8675(805) 289(45) | ? ? ? 348(52) 505(42) 324(29) 367(41) 7548(1241) 21765(1534) 501(67) 596(60) 286(67) |
| Blank test | 5833(49) | ||||
Above data are statistical average numerical value, and the numerical value in the bracket is standard deviation.
7 kinds of unanimities as a result that different chip obtained (result slightly), these results also are consistent with the result that planar chip obtained (result slightly) with present standard.
The biochip that the present invention relates to can the time prevent from mutual seepage between the reaction tank to make chip have only a plane again when scanning in reaction, thus the technical difficulty when having reduced greatly M sample analyzed, and analysis cost is reduced greatly.
Claims (10)
1, a kind of biochip of detachable use is characterized in that: it is by base plate (4), and probe card (3) becomes orifice plate (1), and register pin (13) and non-irreversible sealing mechanism are formed; The probe card (3) that outside surface is printed with M probe array is housed on the base plate (4), become be equipped with on the orifice plate (1) corresponding with the probe array zone on the probe card (3), give reaction tank with M eyelet (16) highly, become orifice plate to be placed on the probe card (3), is connected with pin that pin (13) is formed and makes the entire chip location by being arranged on into pin-and-hole (14) on orifice plate (1) and the base plate (4), make probe card (3) and become the surface of contact formation between the orifice plate (1) not leakage sealed by non-irreversible sealing mechanism; Can install and remove in use as required, when promptly each probe array position isolates mutually on the same chip of needs, be assembled into the structure that to isolate mutually, when not needing to isolate, isolation structure be removed.
2, according to the described biochip of claim 1, it is characterized in that: probe array region on the probe card (3) and border thereof (discrepancy in elevation is less than 0.2mm) all in the same plane, a plurality of independently probe arrays can be arranged, handle a plurality of samples.Probe card with these features is merely able to use in detachable biochip of the present invention.
3, according to the described biochip of claim 1, it is characterized in that: become have on the orifice plate (1) with probe card (3) on corresponding M the eyelet (16) in active region, M probe array place, these eyelets have enough height, to guarantee that M the reaction tank that itself and above-mentioned probe card (3) form has enough height, guarantee application of sample, hatch, cleaning etc. with test relevant being reflected in each reaction tank and independently carry out.
4, according to the described biochip of claim 1, it is characterized in that: the cylindrical sleeve of hollow in spacer sleeve (2) the chamber that to be M made by rubber or plastics with sealing property, place them on the probe card (3) in the need time spent, respectively each probe array ecological segregation is sealed and is embedded in in the eyelet (16) of orifice plate (1), in order to reactant liquor with become orifice plate isolated.
5, according to the described biochip of claim 1, it is characterized in that: its non-irreversible sealing mechanism is by becoming orifice plate (1) under the self gravitation effect, closely be pressed together on the probe card (3), forming the mechanism that M independent mutual impervious reaction tank constituted.
6, according to the described biochip of claim 1, it is characterized in that: its non-irreversible sealing mechanism is by becoming orifice plate (1) under the effect that vacuum tube (5) is vacuumized, closely be adsorbed on the probe card (3), form the mechanism that M independent mutual impervious reaction tank constituted.
7, according to the described biochip of claim 1, it is characterized in that: its non-irreversible sealing mechanism is by becoming orifice plate (1) under screw (7) screwing force effect, closely be pressed together on chip (3) plate, form the mechanism that M independent mutual impervious reaction tank constituted.
8, according to the described biochip of claim 1, it is characterized in that: its non-irreversible sealing mechanism is by becoming orifice plate (1) to act on down at ambient pressure (8), closely be pressed together on the probe card (3), form the mechanism that M independent mutual impervious reaction tank constituted.
9, according to the described biochip of claim 1, it is characterized in that: its non-irreversible sealing mechanism is by becoming orifice plate (1) under the clamping force effect between dop (9) and the draw-in groove (10), closely be pressed together on the probe card (3), form the mechanism that M independent mutual impervious reaction tank constituted.
10, according to the described biochip of claim 1, it is characterized in that: its non-irreversible sealing mechanism is by becoming orifice plate (1) under the magneticaction of magnet [permanent magnet (11) or electromagnet (12)], closely be pressed together on the probe card (3), form the mechanism that M independent mutual impervious reaction tank constituted.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021135401A CN1186635C (en) | 2002-03-29 | 2002-03-29 | Biological chip capable of being disassembled and assembled |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021135401A CN1186635C (en) | 2002-03-29 | 2002-03-29 | Biological chip capable of being disassembled and assembled |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1448723A true CN1448723A (en) | 2003-10-15 |
| CN1186635C CN1186635C (en) | 2005-01-26 |
Family
ID=28680577
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB021135401A Expired - Fee Related CN1186635C (en) | 2002-03-29 | 2002-03-29 | Biological chip capable of being disassembled and assembled |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1186635C (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004083863A1 (en) * | 2003-03-04 | 2004-09-30 | Chengdu Kuachang Medical Industrial Limited | An integrating analysis chip with minimized reactors and its application |
| CN101988921A (en) * | 2009-07-29 | 2011-03-23 | 丹耐克斯技术有限公司 | Sample plate |
| CN1825121B (en) * | 2005-02-24 | 2011-06-08 | 上海裕隆生物科技有限公司 | Plastic chip |
| CN102226147A (en) * | 2011-05-30 | 2011-10-26 | 苏州偲聚生物材料有限公司 | Biochip incubation reactor |
| CN104181293A (en) * | 2014-08-26 | 2014-12-03 | 梧州市红十字会医院 | Enzyme-linked immunosorbent assay method |
| US9523701B2 (en) | 2009-07-29 | 2016-12-20 | Dynex Technologies, Inc. | Sample plate systems and methods |
| CN107922910A (en) * | 2016-07-29 | 2018-04-17 | 苏文弘 | Microfluidic device and application thereof and application method |
| CN108786941A (en) * | 2018-05-26 | 2018-11-13 | 大连大学 | A kind of preparation method of novel magnetic self assembled three-dimensional paper chip |
-
2002
- 2002-03-29 CN CNB021135401A patent/CN1186635C/en not_active Expired - Fee Related
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004083863A1 (en) * | 2003-03-04 | 2004-09-30 | Chengdu Kuachang Medical Industrial Limited | An integrating analysis chip with minimized reactors and its application |
| CN1825121B (en) * | 2005-02-24 | 2011-06-08 | 上海裕隆生物科技有限公司 | Plastic chip |
| US9523701B2 (en) | 2009-07-29 | 2016-12-20 | Dynex Technologies, Inc. | Sample plate systems and methods |
| CN101988921A (en) * | 2009-07-29 | 2011-03-23 | 丹耐克斯技术有限公司 | Sample plate |
| US10969386B2 (en) | 2009-07-29 | 2021-04-06 | Dynex Technologies, Inc. | Sample plate systems and methods |
| CN101988921B (en) * | 2009-07-29 | 2014-05-14 | 丹耐克斯技术有限公司 | Sample plate |
| US9857367B2 (en) | 2009-07-29 | 2018-01-02 | Dynex Technologies, Inc. | Sample plate systems and methods |
| US9244069B2 (en) | 2009-07-29 | 2016-01-26 | Dynex Technologies | Sample plate systems and methods |
| CN102226147A (en) * | 2011-05-30 | 2011-10-26 | 苏州偲聚生物材料有限公司 | Biochip incubation reactor |
| CN102226147B (en) * | 2011-05-30 | 2013-01-23 | 苏州偲聚生物材料有限公司 | Biochip incubation reactor |
| CN104181293A (en) * | 2014-08-26 | 2014-12-03 | 梧州市红十字会医院 | Enzyme-linked immunosorbent assay method |
| CN107922910A (en) * | 2016-07-29 | 2018-04-17 | 苏文弘 | Microfluidic device and application thereof and application method |
| CN108786941A (en) * | 2018-05-26 | 2018-11-13 | 大连大学 | A kind of preparation method of novel magnetic self assembled three-dimensional paper chip |
| CN108786941B (en) * | 2018-05-26 | 2020-11-27 | 大连大学 | Preparation method of novel magnetic self-assembly three-dimensional paper chip |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1186635C (en) | 2005-01-26 |
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