CN1445003A - Method and devices for preparing biology derivation material - Google Patents
Method and devices for preparing biology derivation material Download PDFInfo
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- CN1445003A CN1445003A CN 03117711 CN03117711A CN1445003A CN 1445003 A CN1445003 A CN 1445003A CN 03117711 CN03117711 CN 03117711 CN 03117711 A CN03117711 A CN 03117711A CN 1445003 A CN1445003 A CN 1445003A
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Abstract
A process for preparing the biologic derivative material from the biologic tissue/organ features that a processing medium is used to remove fat, cell, protein and calcium from said tissue/organ. Its apparatus is composed of electric motor, microcomputer to control said motor, washing cage driven by said motor to rotate in two directions for washing the tissue/organ and mixture of said processing medium, and temp controller. The processed material has a certain bioactivity.
Description
Technical field the present invention relates to the bio-derived material manufacturing system, particularly keeps certain biological tissue basic structure, removes the manufacture method and the device thereof of antigenic bio-derived material substantially.
Technical background is more and more because of the damaged patient of tissue/organ that disease, wound cause, and has become modal clinical problem, is to disable and dead major reason.The damaged replacement method for implantation treatment commonly used of tissue/organ in the prior art.Existing alternative comprises xenogenesis, allogeneic, autologous tissue/organ and artificial material.Heteroplasm transplants and causes fastish rejection, is used for the clinical day that also takes; Allohisto compatibility's graft-rejection is inevitable, and organizes limitedly, is not easy to produce in batches; The autograft meeting cause for district's pain, infection, damage and can provide tissue-derived limited, impossible industrialization; Cause foreign body reaction after traditional artificial material implants, secondary infection, exposed and healing are bad etc., and this just makes the scientist go to seek more ideal tissue substituent.Along with continually developing of new biomaterial for medical purpose, brought new hope for the treatment of clinical tissue/organ defect.Require the tissue/organ substitute to possess following condition clinically: excellent biological compatibility be arranged, the wearing and tearing that do not have of permanent substitute; Temporary substitute should have tissue regeneration guided bone/inductivity, requires to have material degradation and synchronous, ideal surface characteristic of tissue regeneration and higher porosity; The mechanical property of substitute and host tissue is complementary.But from the result of clinical trial, that simple inorganic material has is frangible, mechanical property does not match and shortcoming such as healing delay; Simple organic material substitute exists mechanical strength poor, and catabolite is piled up and caused that sour environment is not easy to shortcomings such as organization healing; Inorganic material and organic material compound is subjected to the restriction of material technological level up till now, is difficult to reach the substitute of clinical needs; The synthetic material substitute that is used to repair soft tissue and organ defect is short of very much.
Bio-derived material is to utilize biological tissue/organ, through special handling, removes its antigenicity, keeps the active class material of its basic structure and part biological.This class material not only overcome artificial material existing shortcoming, satisfy clinical practice, and the source is not limited, making is simple, be convenient to batch process, be comparatively ideal at present biomaterial (Dahms SE, Pieiechota HJ, Dahiya R, etal.Composition and biomechanical properties of the bladder acelluarmatrix graft:comparative analysis in rat, pig and human.BritiShJournal of Urology (1998) .82.411-419).But there is following shortcoming in prior biological derived material manufacture method: (1) tissue/organ and treatment media are carried out static treatment, make that treatment media is difficult for infiltrating, mix inhomogeneous, insufficient during processing, the residual composition that should remove down in the tissue/organ body has influenced the performance of the bio-derived material of making.(2) in the processing procedure of tissue/organ, fail fully to guarantee constant temperature (37 ℃) state, make that the biological activity loss of tissue/organ is too many, the bioactive substance of preserving reduces, and is unfavorable for the due inducing action of organizing of bio-derived material performance.(3) in bio-derived material manufacturing process, do not take into full account the saturation problem of removing composition in the treatment media, like this, caused the remaining all the time composition that will remove in the bio-derived material body of handling, it is thorough, insufficient that bio-derived material is handled.(4) prior biological derived material processing method, its processing time is controlled roughly by manual method, the ratio that treatment media accounts for biological tissue/organ also is artificial, the control parameter of technological process has very big difference when making its each processing, the quality that is difficult to the bio-derived material of control treatment is unfavorable for realizing the standardization of production.
Summary of the invention the object of the present invention is to provide a kind of simple in structure, easy to use, by microcomputerized control working time, rotary speed, by temperature controller control temperature, under dynamic, permanent degree condition, to handle thoroughly, fully, have tissue/organ basic structure, remove the method and the device thereof of antigenic bio-derived material substantially.
Manufacture method of the present invention is biological tissue/organ to be removed add treatment media in the device that unwanted portion of tissue is placed on the design and handle, and processing procedure comprises: (1) removes fat; (2) remove lipoprotein and glycoprotein; (3) cell in the removal tissue; (4) remove the inside and outside antigen protein of cell, thereby obtain: have tissue/organ basic structure, remove antigenic bio-derived material substantially,
Above-mentioned biological tissue/organ comprises: (1) soft tissue comprises animal nerve, tendon, ligament, skin, blood vessel, pericardium, cardiac valve, bladder, large intestine, small intestinal; (2) sclerous tissues comprises animal bone, cartilage, tooth; Above-mentioned treatment media comprises: (1) biochemical reagents comprise hydrogen peroxide, trypsin, Collagenase, Triton X-100, Hydrazoic acid,sodium salt, food abluent; (2) organic solvent comprises chloroform, methanol, ethanol, petroleum ether; (3) salt comprises ammonium sulfate, ammonium chloride, sodium carbonate.
The bio-derived material treatment process condition: (1) is shaped and processes: at room temperature use yarn cutting machine and operating theater instruments, and use normal saline flushing, the processing time is 2.0～4.0 hours; (2) cleaning by degreasing: under 37 ℃ in rinse bath with 10%～30% hydrogen peroxide, handled 24～48 hours; (3) rinsing: at room temperature in rinse bath, use rinsed with deionized water 3 times; (4) deproteinization cleans: use chloroform-methanol 3: 1 down at 37 ℃, handled 12-72 hour; (5) rinsing: at room temperature, in rinse bath, use rinsed with deionized water 3 times; (6) lyophilization: under-35 ℃ of conditions in freezer dryer dry 48～72 hours.
The producing device of bio-derived material of the present invention, its structure comprises control section and mechanical part: (1) control section comprises rotating speed control and temperature control two parts, rotating speed control comprises motor, panel control circuit and the display screen of microcomputerized control, can set: rotation cleans that the cage direction of rotation is variable, rotational time and rotating speed adjustable, all parameters are predeterminable; The temperature control part branch comprises: be made up of temperature controller, solid-state relay and electric heating tube three parts.Be provided with electric heating tube in the rinse bath outer lower end,, compare with it by thermocouple sample temperature signal by the temperature controller design temperature, switch by temperature controller control solid-state relay, reach control temperature ascending, descending with this, thereby guarantee the temperature adjusting range: room temperature to 39 ℃, ± 1 ℃ of constant temperature.(2) mechanical part comprises by micro computer, and variable speed electric motors, particularly cleans the cage rotation by driving wheel and rotating shaft driven rotary; Rotation is cleaned the cage two ends and is fixed in the rinse bath with fixed pulley, after rotation cleaning cage removable cover is opened, pending biological tissue and organ can be placed in one, cleanout fluid fully contacts with organ with biological tissue in the cleaning process, rotation is cleaned cage and is provided with handle, it can be taken out rinse bath, the loam cake of rinse bath seals with sealing strip, and be provided with observation window, be convenient to observe rotation and clean the cage duty, after the processing, after fat in the treatment media and protein add fractional distillation medium or precipitating agent by addition funnel and liquid feeding valve, separate, the waste liquid after the separation imports in the waste liquid tank by water discharging valve.
Concrete work is as follows: pack in cage is cleaned in rotation treatment media and fritter biological tissue/organ, the assurance treatment media is cleaned the cage rotation with rotation under Electric Machine Control.Rinse bath loam cake and rinse bath are tight with sealing strip envelope, on be covered with observation window and control panel, can set rotating speed of motor, rotational time, and drive and clean cage along clockwise or counter-clockwise two-way rotation, temperature control guarantees that treatment media is in 37 ℃ of temperature constant states.Fatty, the protein of institute can guarantee to obtain to handle bio-derived material completely in the treatment media after separatory, precipitation purify.Addition funnel is used to add treatment media, separating medium or precipitating liquid.Waste liquid imports waste liquid tank and removes after treatment.
The present invention compared with prior art has following advantage:
(1) cleans the two-way rotation of cage by microcomputerized control motor-driven rotation, make tissue/organ and treatment media carry out dynamic process, guarantee that treatment media is easy to infiltrate, mix homogeneously during processing, fully, the uniformity of the bio-derived material processing that has guaranteed.
(2) in the processing procedure of tissue/organ, temperature controller guarantees the temperature constant state of treatment media, the biological activity loss of the feasible tissue/organ of handling reduces, and preserves more bioactive substance, the due inducing action of organizing of bio-derived material performance that is beneficial to make.
(3) in bio-derived material manufacturing process, fully take into account the saturation problem of removing composition in the treatment media, in rinse bath by fractional distillation or sedimentary method, effectively remove fat, protein composition in the treatment media, like this, the not remaining composition that will remove in the bio-derived material body that has guaranteed to handle, it is thorough, abundant that bio-derived material is handled.
(3) the present invention has adopted the processing method of automatic control, its treatment conditions can accurately be controlled, treatment media accounts for the ratio of biological tissue/organ and also can determine, the control parameter of technological process is basic identical when making its each processing, be convenient to the quality of the bio-derived material of control treatment, be beneficial to the standardization that realizes production.
Below be accompanying drawing drawing explanation of the present invention:
Fig. 1 is the structural front view of producing device of the present invention.
Fig. 2 is the structure vertical view of producing device of the present invention.
Fig. 3 is the left view that producing device of the present invention cleans cage and rinse bath.
Fig. 4 is the structural representation that cage is cleaned in producing device rotation of the present invention.
Fig. 5 is bio-derived material processing technological flow figure of the present invention.
Fig. 6 is a bio-derived material cleaning by degreasing process chart of the present invention.
Fig. 7 is bio-derived material deproteinization cleaning process flow figure of the present invention.
Fig. 8 is a rotating-speed control circuit theory diagram of the present invention.
Fig. 9 is a temperature-control circuit theory diagram of the present invention.
Specific embodiment the present invention is described in further detail below with reference to embodiment and accompanying drawing:
1. 1 kinds of bio-derived material manufacture methods of embodiment: be biological tissue/organ to be removed add treatment media in the device that unwanted portion of tissue is placed on the design and handle, processing procedure comprises: remove fat 1.; 2. remove lipoprotein and glycoprotein; 3. remove the cell in the tissue; 4. the inside and outside antigen protein of removal cell, thus obtain: have tissue/organ basic structure, remove antigenic bio-derived material substantially,
Above-mentioned biological tissue/organ comprises: 1. soft tissue comprises animal nerve, tendon, ligament, skin, blood vessel, pericardium, cardiac valve, bladder, large intestine, small intestinal; 2. sclerous tissues comprises animal bone, cartilage, tooth;
Above-mentioned treatment media comprises: 1. biochemical reagents comprise hydrogen peroxide, trypsin, Collagenase, Triton X-100, Hydrazoic acid,sodium salt, food abluent; 2. organic solvent comprises chloroform, methanol, ethanol, petroleum ether; 3. salt comprises ammonium sulfate, ammonium chloride, sodium carbonate.
The process conditions that bio-derived material processing, cleaning by degreasing, deproteinization clean are listed as follows (referring to monologue story-telling with gestures accompanying drawing 5,6,7) respectively:
Table 1 bio-derived material treatment process condition processing apparatus or solution-treated temperature (℃) yarn cutting machine is processed in processing time (h) shaping and the operating theater instruments physiological water circulates towards room temperature 4-6
Wash-out fat cleans dynamic cleaning groove and hydrogen peroxide (10%～30%) 37 24-48 rinsing dynamic cleaning grooves and deionized water room temperature and takes off albumen cleaning dynamic cleaning groove and chloroform-methanol (3: 1) 37 12-72 rinsing dynamic cleaning grooves and 3 freeze drying freeze driers-35 of deionized water room temperature 48-72 for 3 times
Table 2 bio-derived material cleaning by degreasing process conditions processing apparatus or solution-treated temperature (℃) processing time (h) dynamically cleans dynamic rinse bath and hydrogen peroxide (10%～30%) 37 24-48 extract petroleum ether 37 3 times
Table 3 bio-derived material deproteinization cleaning condition processing apparatus or solution-treated temperature (℃) processing time (h) dynamically cleans dynamic rinse bath and chloroform-methanol (3: 1) 37 36-48 precipitating precipitating agent 37 3 times
Embodiment 2. is referring to Figure of description 1-4,8 and 9, a kind of bio-derived material producing device, its structure comprises control section and mechanical part: 1. control section comprises rotating speed control and temperature control two parts: (1) rotating speed control section is the direction of rotation of cleaning the cage motor by microprocessor control rotation, rotational time and rotating speed, keyboard provides the instruction of various parameters to microprocessor by the keyboard monitoring interface, and on the liquid crystal device, show, control section can be set following parameter: the cage direction of rotation is cleaned in rotation, rotational time and rotating speed adjustable, all parameters are predeterminable.(2) the temperature control part branch comprises: be made up of temperature controller (19) and solid-state relay (20) and electric heating tube (21) three parts, be provided with electric heating tube in the rinse bath outer lower end, by the temperature controller design temperature, compare with it by thermocouple sample temperature signal, switch by temperature controller control solid-state relay, reach control temperature ascending, descending with this, thereby guarantee the temperature adjusting range: room temperature to 39 ℃, ± 1 ℃ of constant temperature.2. mechanical part comprises by micro computer (1) control variable speed electric motors, particularly (2), cleans cage (5) rotation by driving wheel (3) and rotating shaft (4) driven rotary; Rotation is cleaned the cage two ends and is fixed in the rinse bath (7) with fixed pulley (6), after rotation cleaning cage removable cover (8) is opened, pending biological tissue and organ (9) can be placed in one, cleanout fluid in the cleaning process (10) fully contacts with organ with biological tissue, rotation is cleaned cage and is provided with handle (11), it can be taken out rinse bath, the loam cake of rinse bath (12) is provided with observation window (13), be convenient to observe rotation and clean the cage duty, loam cake and rinse bath seal with sealing strip (14), after fat in the treatment media and protein add fractional distillation medium or precipitating agent by addition funnel (15) and liquid feeding valve (16), separate, the waste liquid after the processing imports in the waste liquid tank (18) by flowing water Valve (17).
Embodiment 3: the making of part deproteinization bio-derived bone.Get 2 kilograms of fresh pig ribs of butchering, cut the back open and remove soft tissue and bone marrow, be processed into 2-5 centimetre meropodium.Place bio-derived material manufacturing system rinse bath, dynamically cleaned 1 hour for 4 liters, remove surface fat and other impurity with containing food abluent 10% physiological salt liquid; Handled 48 hours with 4 liters of 30% hydrogen peroxide dynamic circulation under 37 ℃ of conditions, slough the fat in the rib, use the petroleum ether fractionation of fatty; Clean 3 times with physiological salt liquid; Add 4 liters of chloroform-methanols (3: 1) dynamic circulation under 37 ℃ of conditions then and handled 72 hours, the part deproteinization is removed protein in the chloroform-methanol with ammonium sulfate; Clean 3 times with physiological salt liquid; Obtain part deproteinization bio-derived bone at last, after lyophilizing is sealed up for safekeeping, be stored in the cryogenic refrigerator.
Embodiment 4: the making of biologically-derived decalcification bone.Get 2 kilograms of fresh pig ribs of butchering, cut the back open and remove soft tissue and bone marrow, be processed into 2-5 centimetre long section.Place bio-derived material manufacturing system rinse bath, dynamically cleaned 1 hour for 4 liters, remove surface fat and other impurity with containing food abluent 10% physiological salt liquid; Handled 48 hours with 4 liters of 30% hydrogen peroxide dynamic circulation under 37 ℃ of conditions, slough the fat in the rib, use the petroleum ether fractionation of fatty; Clean 3 times with physiological salt liquid; Add the dynamic circulation decalcification 60 hours under room temperature of 4 liters of 0.6M hydrochloric acid then, precipitate calcium salt with sodium carbonate; Go in the ethanol and dynamically cleaned 4 hours, remove residual hydrogen peroxide; Handled 24 hours with 4 liters of chloroform-methanols (3: 1) dynamic circulation under 37 ℃ of conditions, the part deproteinization is removed protein in the chloroform-methanol with ammonium sulfate; Obtain biologically-derived decalcification bone at last, after lyophilizing is sealed up for safekeeping, be stored in the cryogenic refrigerator.
Embodiment 5: the making of bio-derivative tendon.Get the fresh pig of butchering and play 0.5 kilogram of tendon, remove surperficial aponeurosis (aponeuroses), make 4 centimetres long section.Place bio-derived material manufacturing system rinse bath, dynamically cleaned 1 hour for 1 liter, remove surface fat and other impurity with containing food abluent 10% physiological salt liquid; Handled 12 hours with 1 liter of 10% hydrogen peroxide dynamic circulation under 37 ℃ of conditions, slough the fat of playing in the tendon, use the petroleum ether fractionation of fatty; Clean 3 times with physiological salt liquid; Add 1 liter of chloroform-methanol (3: 1) dynamic circulation under 37 ℃ of conditions then and handled 24 hours, further slough cell and the fat played in the tendon, clean 3 times with physiological salt liquid; Obtain bio-derivative tendon at last, after lyophilizing is sealed up for safekeeping, be stored in the cryogenic refrigerator.
Embodiment 6: the making of biologically-derived bladder.Get one of fresh Vesica sus domestica of butchering, cut open, get the first half from equator, tear mucosa after, be placed in the bio-derived material manufacturing system rinse bath.Dynamically cleaned 1 hour for 1 liter with containing food abluent 10% physiological salt liquid earlier, remove surface fat and other impurity; Handled the part cell in the dissolved matrix 16 hours with 1 liter of 10mmol/L normal saline (containing 0.1% Hydrazoic acid,sodium salt) dynamic circulation under 37 ℃ of conditions; Clean 3 times with physiological salt liquid; Handled 24 hours with the solution that contains 1mol/LNaCl and DNA enzyme dynamic circulation under 37 ℃ of conditions then, further dissolved cell is also removed composition in all cells; Then continue dynamic circulation processing 12 hours, to remove cell membrane lipid bilayer and intracellular membrane fat with containing 4% NaTDC (containing 0.1% Hydrazoic acid,sodium salt) solution.Clean 3 times with physiological salt liquid at last, obtain biologically-derived bladder, after lyophilizing is sealed up for safekeeping, be stored in the cryogenic refrigerator.
Claims (2)
1. bio-derived material manufacture method: it is characterized in that: biological tissue/organ removed add treatment media in the device that unwanted portion of tissue is placed on the design and handle, processing procedure comprises: (1) removes fat; (2) remove lipoprotein and glycoprotein; (3) cell in the removal tissue; (4) remove the inside and outside antigen protein of cell, thereby obtain: have tissue/organ basic structure, remove antigenic bio-derived material substantially, 1.1 above-mentioned biological tissue/organ comprises: the 1.1.1 soft tissue comprises animal nerve, tendon, ligament, skin, blood vessel, pericardium, cardiac valve, bladder, large intestine, small intestinal; 1.1.2 sclerous tissues comprises animal bone, cartilage, tooth; 1.2 above-mentioned treatment media comprises: the 1.2.1 biochemical reagents comprise hydrogen peroxide, trypsin, Collagenase, Triton X-100, Hydrazoic acid,sodium salt, food abluent; 1.2.2 organic solvent comprises chloroform, methanol, ethanol, petroleum ether; 1.2.3 salt comprises ammonium sulfate, ammonium chloride, sodium carbonate; The processing 1.3 bio-derived material treatment process condition: 1.3.1 is shaped: at room temperature with yarn cutting machine or operating theater instruments, and use normal saline flushing, the time is 2.0～4.0 hours; 1.3.2 cleaning by degreasing: the hydrogen peroxide with 10%-30% dynamically cleaned 24～48 hours in rinse bath under 37 ℃; 1.3.2.1 extract fat 3 times with petroleum ether down at 37 ℃, changed hydrogen peroxide once every 24 hours; Dry hydrogen peroxide 1.3.2.2 at room temperature clean cage, obtain defat biological tissue with rotation; 1.3.3 rinsing: at room temperature in rinse bath, use rinsed with deionized water 3 times; 1.3.4 deproteinization cleans: use chloroform-methanol 3: 1 down at 37 ℃, handled 12-72 hour; 1.3.4.1 using ammonium sulfate 4mol/L, pH7.0 to saltout down at 37 ℃ contains albumen and fatty treatment media, removes albumen and fat 3 times; Dry chloroform-methanol 3 times 1.3.4.2 at room temperature clean cage, obtain defat, deproteinization biological tissue with rotation; 1.3.5 rinsing: at room temperature, in rinse bath, use rinsed with deionized water 3 times;
2. implement the bio-derived material producing device of claim 1, its structure is characterised in that: comprise control section and mechanical part: 2.1 control sections comprise rotating speed control and temperature control two parts, 2.1.1 the rotating speed control section is direction of rotation, rotational time and the rotating speed that is cleaned the cage motor by microprocessor control rotation, keyboard provides the instruction of various parameters by the keyboard monitoring interface to microprocessor, and shows on liquid crystal display; 2.1.2 rotation is cleaned, and the cage direction of rotation is variable, rotational time and rotating speed adjustable, it is predeterminable that cage direction of rotation, rotational time and rotating speed are cleaned in the 2.1.3 rotation; 2.2 the temperature control part branch comprises: form by temperature controller (19) and solid-state relay (20) and electric heating tube (21) three parts, be provided with electric heating tube in the rinse bath outer lower end, by the temperature controller design temperature, compare with it by thermocouple sample temperature signal, switch by temperature controller control solid-state relay, reach control temperature ascending, descending with this, thereby guarantee the temperature adjusting range: room temperature to 39 ℃, ± 1 ℃ of constant temperature; 2.3 mechanical part comprises by micro computer (1) control variable speed electric motors, particularly (2), cleans cage (5) rotation by driving wheel (3) and rotating shaft (4) driven rotary; Rotation is cleaned the cage two ends and is fixed in the rinse bath (7) with fixed pulley (6), after rotation cleaning cage removable cover (8) is opened, pending biological tissue and organ (9) can be placed in one, cleanout fluid in the cleaning process (10) fully contacts with organ with biological tissue, rotation is cleaned cage and is provided with handle (11), it can be taken out rinse bath, the loam cake of rinse bath (12) is provided with observation window (13), be convenient to observe rotation and clean the cage duty, loam cake and rinse bath seal with sealing strip (14), fat in the treatment media and protein, after addition funnel (15) and liquid feeding valve (16) adding fractional distillation medium or precipitating agent, separate, the waste liquid after the separation imports in the waste liquid tank (18) by flowing water Valve (17); 2.3.1 it is 2mm-6mm that the aperture, side of cage is cleaned in rotation, guarantees that the inside and outside treatment media of cage is with easy access, guarantees that simultaneously cage inner tissue organ does not spill.
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