CN1325906C - Portable multifunctional electrochemical biological detecting instrument - Google Patents
Portable multifunctional electrochemical biological detecting instrument Download PDFInfo
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- CN1325906C CN1325906C CNB021571848A CN02157184A CN1325906C CN 1325906 C CN1325906 C CN 1325906C CN B021571848 A CNB021571848 A CN B021571848A CN 02157184 A CN02157184 A CN 02157184A CN 1325906 C CN1325906 C CN 1325906C
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Abstract
The present invention relates to a portable multifunctional electrochemical biologic detecting instrument which comprises a plurality of concentration sensors, a plurality of external information memories and a multifunctional signal analysis processor. Each concentration sensor has a reacting region. A substance which can react with a corresponding target object to be measured can be placed on the reacting region which is provided with at least two independent electrodes which are not connected mutually, wherein one electrode is a reference electrode, and the other electrode is a work electrode. When detection reaction is generated, the work electrode can output an electrochemical reaction signal.
Description
Technical field
The present invention is about a kind of Portable multifunctional bio detector, particularly relevant for a kind of Portable multifunctional bio detector that detects the concentration of plurality of target determinand.Portable multifunctional bio detector of the present invention at every turn can a pair of concentration sensor detects the concentration of a corresponding target determinand with the external information internal memory, and calculate the concentration of the target determinand of above-mentioned correspondence by a multifunctional signal analysis processor, and will detect its result's output, simultaneously can cooperate a state detecting device, whether normal in order to the state of detecting Portable multifunctional bio detector.
Background technology
The Portable bio-detector mainly is to be used for accurate large-scale checkout equipment and the difficult detection site that arrives of operating personnel, measure as remote local researchs such as field mountain forests, or provide common people to be convenient to fast detecting, as house or fixed point nursing detections such as blood sugar, cholesterol, protoheme and uric acid.The Portable bio-detector is the most common with optical profile type and electric chemical formula two big classes.Because the large-scale checkout equipment of most of existing precision, use optical measuring, so the portable optical bio-detector is also comparatively general more, as the blood glucose meter of early-stage development promptly be.The optical biologic detector cooperates light source and light detection device because of need, can't with detector again microminiaturization to be convenient for carrying, also because of its assembly easily along with the time instability that becomes that wears out, moreover disturbed by external light source and produce error.The development of electrochemica biological detector can improve this shortcoming, and its advantage not only overcomes outside the aforesaid drawbacks, and relatively the survey concentration sensor of both uses is on commercial production, and the cost of manufacture that galvanochemistry is surveyed concentration sensor is more cheap.Therefore, the portable optical bio-detector is progressively replaced by electric chemical formula.
Though the advantage of Portable electrochemica biological detector is many, still have its shortcoming to wait to improve, mainly be the electrochemical signals that transmits on the electrode, meeting is difference because of the singularity of surveying concentration sensor.This is by the preparation of the every batch of medicament or use reasons such as error of the activity of ferment to be caused, make is not all every batch survey concentration sensor production after, the detection of same concentrations is all had the same electrical chemical signal.Therefore, need have the detection of this batch survey concentration sensor characteristic and the parameter of calculation correction, allow similar concentration sensor can apply to identical electrochemica biological detector, the error between reduction batch by outside input.In the mode of outside input parameter control electrochemica biological detector, just, utilize software program and the processor both deposited to carry out computing, again with its result's output as in the data input computing machine that disk is stored.With the more utilization of microminiaturization of this notion, just as hand held card insert type game machine.U.S.Patent No.4,975,647 are a bio-analysis instrument of utilizing outside input parameter to control, this is stated as relevant for the matching used test group of a blood analyser, the parameter of definition of data such as the mensuration correction opportunity that it was suitable for, the container of fluid and concentration, be established in the extrapolation storage card that an inside is provided with the parameter checksum, reach the invention of accurate detection by this.U.S.Patent No.5,366,609 is a kind of example that utilizes the Portable electrochemica biological detector of outside input parameter control, the inventive features of this patent is: 1) utilize predetermined Cottrell current relation formula (U.S.Patent No.5,366, in 609 and 5,243,516 narration is also arranged):
N=transmission electronic number; The F=Faraday constant; A=measures electrode area
The C=analyte concentration; The coefficient of diffusion of D=electroactive material; The t=time
Push back formula with it:
With the electrochemical current analog signal of a period of time inner analysis thing (glucose), at a time between in the interval, constantly detect with cumulative data after, convert the digital signal output of analyte (glucose) concentration to through microprocessor etc.; 2) utilize one store the extrapolation storage card of related assays parameter and microprocessor between, several opportunitys when detecting, utilize the mechanism of constantly checking repeatedly, confirm that the extrapolation storage is stuck in testing process and whether still exists and maybe may be replaced, to avoid the measures of input error parameter.The computing formula of this invention and measures need the design collocation of more loaded down with trivial details and accurate software program and nextport hardware component NextPort, and the cost of its enforcement must obviously reduce.
Portable electrochemica biological detector in the market, still with single machine and single function can detection, as Boehringer Mannheim, Roche, MediSense, the blood glucose meter of Matsushita etc. and blood sugar concentration test pieces, these manufacturers all do not have the Portable electrochemica biological detector of producing the marketing multi-functional machine as yet.The present invention by multiple in order to the preparation of detectable concentration medicament or ferment sensor, survey concentration sensor (TaiWan, China patent of invention card number 109466 and 124332) as blood sugar, uric acid is surveyed concentration sensor (TaiWan, China patent of invention card numbers 107073 and United States Patent (USP) card US6,258,230B1), protoheme is surveyed concentration sensor (TaiWan, China patent announcement numbers 466344 and Application No. 09/771,634) etc., the detection of corresponding outside input with it cooperates with the parameter of calculation correction, not only simplify the design of software program and nextport hardware component NextPort, can reach the concentration of accurate detection object, more the concentration of multiple specific objective thing can be detected, be integrated in a Portable detector, so that implementation cost can obviously reduce, become the Portable multifunction electric chemical-biological detector of a tractor serves several purposes.
Because the Portable multifunction electric chemical-biological detector after integrating, must be able to allow the operator whether normally can confirm at any time to the state of detector, therefore the present invention designs the state detecting device of a fixed resistance value resistance simultaneously, whether the state that just can detect detector by this easily is normal, to increase the accuracy of Portable electrochemica biological detector integral body.
Summary of the invention
One object of the present invention is, by electrochemical principle with the multinomial detection components that can be used for detecting object concentration, be integrated into a multi-functional bio-detector, and by with the external information internal memory of concentration sensor coupling, during with detectable concentration sensor parameters needed information stores wherein so that the function of detector conversion; The external information internal memory of concentration sensor coupling, both combine with the multifunctional signal analysis processor, become the electric chemical formula bio-detector with multi-functional machine, and the volume operation that lightly is easy to carry.
For this reason, the invention provides a kind of Portable multifunction electric chemical formula bio-detector, this detector comprises: a plurality of concentration sensors, each concentration sensor has a reaction zone, a reference electrode and a working electrode, wherein these electrodes are independent and do not link to each other mutually, on this reaction zone, can put one can be corresponding with one the material of target determinand reaction when detection reaction takes place, working electrode can be exported an electrochemical reaction signal; A plurality of external information internal memories, it corresponds respectively to this a plurality of concentration sensors, and each corresponding external information internal memory can provide the parameter information in order to the concentration of analyzing this corresponding target determinand; One multifunctional signal analysis processor, it has a microprocessor, an one electronics erasable programmable read/write memory and an environment temperature sensor, this multifunctional signal analysis processor has two external input terminals at least, can link to each other with this external information internal memory with this concentration sensor respectively, this microprocessor all changes the parameter information in this external information internal memory over to earlier to this electronics erasable programmable read/write memory, making microprocessor only adopt internal information to carry out detects, and when electrochemical reaction, the parameter information that the electrochemical reaction signal that can export by this sensor and this electronics erasable programmable read/write memory are provided, and combination is in order to set up this temperature sensor of a temperature compensation, calculating the concentration of this corresponding target determinand, and with its testing result output; Wherein this detector one group of concentration sensor of arranging in pairs or groups mutually of each use and external information internal memory detect the concentration of corresponding target determinand, can detect the concentration of different target determinand by this.
By microprocessor in the multifunctional signal analysis processor and electronics erasable programmable read/write memory, change the parameter of external information internal memory over to electronics erasable programmable read/write memory, making microprocessor only adopt internal information to carry out detects, up to the parameter of carrying out new external information internal memory, the parameter of electronics erasable programmable read/write memory just is updated, and utilize the parameter checksum to confirm that the parameter input is errorless, to guarantee the unlikely loss of detected parameters information.
Electrode grounding by concentration sensor becomes reference electrode, another outside of serving as reasons provides a reference voltage to make the working electrode of its signal through amplifier and feedback resistance amplifying signal, the electrochemical reaction signal of when detecting, working electrode being exported, to supply with this multifunctional signal analysis processor, calculate the concentration of target determinand.
Be connected to working electrode by the non-positive input that has one group of adjustable amplifier of multiplying power in the multifunctional signal analysis processor, and can provide a fixed voltage to working electrode; In target determinand and reactant effect at the beginning after, interruption provides voltage, and after the detection stand-by period of appointment in the electronics erasable programmable read/write memory of wait multifunctional signal analysis processor, provide a fixed voltage again to working electrode, to form the electrochemical reaction environment.This moment is by the signal of working electrode output electrochemical reaction, and with the parameter detecting of appointment in the electronics erasable programmable read/write memory of multifunctional signal analysis processor, will from working electrode detect signal rationally amplify, again via the computing method parameter of appointment in the information internal memory, calculate the concentration of target determinand, and testing result is exported.
Another object of the present invention is to, by a state detecting device in the detector, whether the state that can detect detector easily is normal, makes things convenient for the user to detect the detector state at any time, to increase the accuracy of Portable electrochemica biological detector integral body.
Description of drawings
Fig. 1 is the electrochemical signals and the uric acid concentration graph of a relation of known uric acid sensor.
Fig. 2 is a multifunction electric chemical formula bio-detector of the present invention.
Fig. 3 is external information card structure figure.
Fig. 4 is concentration sensor of the present invention and state detecting device structural map, and wherein concentration sensor contains reaction zone, electrode, electrode; In addition, contain two electrodes and a resistance on the PCB of state detecting device.
Fig. 5 is the functional block diagram of multifunctional signal analysis processor of the present invention.
Fig. 6 is a signal enlarger composition of the present invention.
The signal enlarger composition that Fig. 7 extends for the present invention.
Fig. 8 is a software flow pattern of the present invention.
Fig. 9 detects figure for blood sugar concentration of the present invention.
Figure 10 detects figure for uric acid concentration of the present invention.
Figure 11 detects figure for protoheme concentration of the present invention.
Figure 12 detects figure for total cholesterol concentration of the present invention.
Figure 13 detects figure for lactic acid concn of the present invention.
The element numbers explanation
1 multifunctional signal analysis processor
2 external information memory banks
3 concentration sensor slots
4 buttons
5 display panels
6 external information cards
7 concentration sensors
8 reaction zones
9 electrodes
10 electrodes
11 enclosing covers
12 enclosing covers
13 PCB
14 external information internal memories
15 Chip electric capacity
16 leads
17 state detecting devices
The 18-1 electrode
The 18-2 electrode
19 fixed resistance value resistance
20 microprocessors
21 electronics erasable programmable read/write memories
22 electric power controllers
23 reference voltage unit
24 concentration sensors insert detecting unit
25 signal amplification units
26 access selected cells
27 environment temperature sensors
28 simulations are to digital switching device
29 value of reading unit selecting arrangements
30 display device
The 31 storage value of reading devices
32 buses
33 amplifiers
34 wiring
35 wiring
36 wiring
37 change-over switches
38 feedback resistances
39 feedback resistances
40 wiring
41 wiring
42 wiring
43 wiring
44 amplifiers
45 resistance
46 resistance
47 resistance
48 resistance
49 change-over switches
50 change-over switches
51 change-over switches
52 change-over switches
53 wiring
54 wiring
55 wiring
56 wiring
57 wiring
58 wiring
59 wiring
60 wiring
61 wiring
62 wiring
63 arrows
74 arrows 74
S device to be measured
The L1 lead
The L2 lead
Embodiment
Control electrochemica biological detector with microprocessor by outside input parameter, it is a known technology, the present invention is integrated into a multi-functional bio-detector for multinomial detection components (that is: a plurality of concentration sensors, a plurality of external information internal memory and a multifunctional signal analysis processor) with electrochemical principle detection object concentration.Simultaneously by respectively with the corresponding a plurality of external information internal memories of above-mentioned a plurality of concentration sensors, above-mentioned a plurality of sensor parameters needed information are stored in respectively wherein during with the concentration that detects the different target determinand, so can detect the concentration of different types of target determinand.A plurality of concentration sensor of the present invention, (ask for an interview TaiWan, China patent of invention card numbers 107073 and United States Patent (USP) card US6.258 as blood sugar concentration sensor (ask for an interview TaiWan, China patent of invention card numbers 109466 and TaiWan, China patent of invention card number 124332), uric acid concentration sensor, 230B1), protoheme concentration sensor (asking for an interview TaiWan, China patent announcement numbers 466344 and Application No. 09/771,634) etc.Be found in suitable electrochemical reaction time following (300 millivolts) by BAS CV-50W voltammetric analyzer, the electrochemical current signal of unitary determination (back is measured for the 7th second as switching on), can calculate electrochemical source of current value and testing concentration between relation.For example, among Fig. 1 shown uric acid concentration sensor in the record result's of voltammetric analyzer curve map.That is electric current and testing concentration between have the relational expression of linear equation in two unknowns formula, therefore when setting outside the detection that should import and calculation correction parameter, with
i＝kC+d
The i=electrochemical source of current; The C=testing concentration
The correction factor 1 of k=determinand; The correction factor 2 of d=determinand
Just can detect the concentration of target determinand.This result's characteristic is: (1) not only need not estimate foundation as testing concentration by the Cottrell current relation formula, (2) and need not continue the detecting with cumulative data detect with computing (as U.S.Pat.No.5,366,609 and 5, narration in 243,516).So, not only simplify the design of software program and nextport hardware component NextPort, can reach the concentration of accurate detection object, more the concentration of multiple specific objective thing can be detected, be integrated in a Portable detector, to become the Portable multifunction electric chemical-biological detector of a tractor serves several purposes.In addition, multi-functional integration of the present invention can make implementation cost significantly reduce.
As shown in Figures 2 and 3, multifunction electric chemical formula bio-detector of the present invention comprises: a plurality of concentration sensors 7; Each concentration sensor 7 has 8, one reference electrodes of a reaction zone and a working electrode, and wherein these electrodes 9,10 are independent and do not link to each other mutually, on this reaction zone, can put one can be corresponding with one the material of target determinand reaction; When detection reaction took place, working electrode can be exported an electrochemical reaction signal; A plurality of external information internal memories 14, it corresponds respectively to this a plurality of concentration sensors 7, and when detecting, each corresponding external information internal memory 14 is contained in the external information card 6, and the parameter information in order to the concentration of analyzing this corresponding target determinand can be provided; One multifunctional signal analysis processor 1, it has a microprocessor 20, an one electronics erasable programmable read/write memory 2 and an environment temperature sensor 27, this multifunctional signal analysis processor 1 has two external input terminals at least, can link to each other with this external information internal memory 14 with this concentration sensor 7 respectively, this microprocessor all changes the parameter information in this external information internal memory over to earlier to this electronics erasable programmable read/write memory 21, so when electrochemical reaction, the parameter information that the electrochemical reaction signal that can be exported by this sensor 7 and this electronics erasable programmable read/write memory 21 are provided, and combination is in order to set up this temperature sensor 7 of a temperature compensation, calculating the concentration of this corresponding target determinand, and will detect its result's output; And a state detecting device 17, it includes two absolute electrode 18-1,18-2, and these absolute electrodes are connected by the resistance 19 of a fixed resistance value.This detector 17 can link to each other with the concentration sensor link that is connected this multifunctional signal analysis processor 1, measure this fixed resistance value resistance 19 by this multifunctional signal analysis processor 1, be built in the resistance of this processor in whether meeting, as the whether normal foundation of this multifunctional signal analysis processor state.Each a pair of concentration sensor 7 of use of this detector and external information internal memory 14 detect the concentration of a corresponding target determinand, can detect the concentration of different target determinand by this.
If concentration sensor 7 is during utilization ferment catalysis system, reactive material can comprise: (1) can with the ferment or the required a series of ferment of subsequent reactions of target determinand reaction, (2) have the electron mediator of redox active, but as when using non-ferment system, then do not have (1).With these reactive materials, the carrier that utilizes hydrophilic macromolecule to form is fixed in the electrochemical reaction district of concentration sensor with reactive material, and has two mutual disjunct electrodes of independence at least in this district, and wherein one is reference electrode, and another is a working electrode.When detection reaction takes place, can provide under the electrochemical reaction environment, by the signal of working electrode output electrochemical reaction, and, make detector detect the different target determinand according to the kind of reactant on the sensor.
As shown in Figure 3, this external information card 6 contains an external information internal memory 14, other conventional detected parameters such as activating threshold values, target determinand identification code, determination step, minute, temperature compensation, parameter sum total check code, computing method is contained in its inside, uses when providing multifunctional signal analysis processor 1 to calculate the target testing concentration.This external information internal memory 14 can be ROM (read-only memory) (ROM), ultraviolet erasing internal memory (UVEPROM), or is electronics erasable programmable read/write memory (EEPROM).
Concentration sensor 7 and state detecting device 17 outward appearance comparison charts as shown in Figure 4, wherein the spacing of 10 at electrode 9 electrodes equates with spacing between electrode 18-1 electrode 18-2.The usefulness of the simple and easy state detecting that act as multifunctional signal analysis processor 1 of state detecting device 17.
The function of multifunctional signal analysis processor 1 is illustrated by Fig. 5, data in the external information internal memory 14 can be passed through bus 32 with data read in the external information internal memory 14 by microprocessor 20, the data storing that reads in electronics erasable programmable read/write memory 21, is calculated the data that read by external information internal memory 14 simultaneously and obtained the parameter summation.With the comparison of the parameter checksum of this parameter summation and external information internal memory 14, whether the data of judging external information internal memory 14 mistake.One concentration sensor 7 can insert detecting unit 24 by concentration sensor and check whether a concentration sensor 7 has the concentration sensor of insertion slot 3.Microprocessor 20 receives detecting unit 24 signals by bus 32, to determine concentration sensor 7 whether the concentration sensor of insertion slot 3 is arranged, if received signal is a low level, then wakes multifunctional signal analysis processor 1 immediately.Microprocessor 20 is by bus 32 control access selected cells 26.Access selected cell 26 makes the voltage level of reference voltage unit 23 be sent to simulation to digital switching device 28.Microprocessor 20 is obtained the output numerical value of simulation to digital switching device 28 by bus 32 again, and judges whether the voltage level of reference voltage unit 23 is unusual.Concentration sensor 7 can obtain the accurate position of suitable polarizing voltage by reference voltage unit 23, and via signal amplification unit 25 concentration sensor 7 reaction signals is amplified, and enlargement ratio is selected via access selected cell 26.This amplifying signal is sent to simulation to digital switching device 28 by the output terminal of signal amplification unit 25 by access selected cell 26.Microprocessor 20 is simulated digital switching device 28 by bus 32 controls.Then, obtain simulation to the row operation of going forward side by side of the output valve of digital switching device 28.Environment temperature sensor 27 is obtained environment temperature, and its numerical value that corresponds to environment temperature is sent to simulation to digital switching device 28 via access selected cell 26.Microprocessor 20 is simulated digital switching device 28 by bus 32 controls.Then, obtain the output valve of simulation, the row operation and show whether present environment temperature has too high or too low situation of going forward side by side to digital switching device 28.After microprocessor 20 is obtained the data of electronics erasable programmable read/write memory 21, by bus 32 control reference voltage unit 23, reference voltage unit 23 provide appropriate voltage position standard to concentration sensor 7, signal amplification unit 25, simulation to digital switching device 28, environment temperature sensor 27.The power supply of each unit of electric power controller 22 management as: microprocessor 20, electronics erasable programmable read/write memory 21, external information internal memory 14, reference voltage unit 23, signal amplification unit 25, simulation are to digital switching device 28.Which kind of unit the value of reading unit selecting arrangement 29 selects measure blood for the user.Microprocessor 20 is by the signal of bus 32 with the unit's of reading selecting arrangement 29.Display device 30 shows various information and test result by bus 32.The measuring value that is stored in the electronics erasable programmable read/write memory 21 can show the storage value of reading by the storage value of reading device 31.
Electrochemical signal enlarger as shown in Figure 6, signal amplification unit 25 is to the structural map of access selected cell 26, its inside is contained an amplifier 33, and is switched switch 37, a feedback resistance 38, a feedback resistance 39, an access selected cell 26.In addition, a device S to be measured (can be concentration sensor 7 or state detecting device 17), lead L1 (electrode 10 of indicated concentration sensor 7 or the electrode 18-2 of state detecting device 17), lead L2 (electrode 9 of indicated concentration sensor 7 or the electrode 18-1 of state detecting device 17).Wiring 35 connects the non-forward end of amplifier 33 and the lead L2 of device S to be measured, and a tip node of change-over switch 37 is connected with wiring 35 through wiring 36 with a tip node of feedback resistance 39.The other end contact of change-over switch 37 is connected with a tip node of feedback resistance 38 through wiring 41 with the other end contact of feedback resistance 39.The other end contact of feedback resistance 38 is connected to wiring 42, and the control end points of change-over switch 37 is connected to access selected cell 26 through wiring 40.The lead L1 of device S to be measured is through wiring 43 ground connection.Reference voltage unit 23 is received wiring 34, and provide the suitable current potential of lead L2 of device to be measured S, this current potential will cause an electric current, current path by the lead L2 of device to be measured S to the reaction zone 8 of concentration sensor 7 or the fixed resistance value resistance 19 of state detecting device, and then to the lead L1 of device S to be measured to ground connection.This electric current amplifies through amplifier 33 and feedback resistance 38 or feedback resistance 39.Amplifying signal is sent to access selected cell 26 by lead 42.Before measuring amplifier 33 output signals, access selected cell 26 send the control end points of a voltage level to change-over switch 37, closing or opening with control its switch 37 via wiring 40.When change-over switch 37 closures, feedback path has only feedback resistance 38, and measure pattern for blood sugar this moment.When change-over switch 37 is opened, feedback path is that feedback resistance 38 adds feedback resistance 39, is uric acid measurement pattern this moment.Access selected cell 26 can be selected and by various simulating signals, these simulating signals is sent to simulation to digital switching device 28.Microprocessor 20 is by bus 32 control access selected cells 26.When concentration sensor 7 or state detecting device 17 inserts concentration sensor slots 3, different electric currents flow through reaction zone 8 or fixed resistance value resistance 19 will be arranged, judge it is that concentration sensor 7 or state detecting device insert according to this.If the state detecting device inserts concentration sensor slot 3, the electric current of the fixed resistance value resistance 19 of then will flowing through amplifies, and this value of reading is compared by the standard upper lower limit value that microprocessor 20 calculates in back and the internal memory.If the state detecting device is normal, when test value surpasses standard bound in the internal memory, can be as the whether unusual simple and easy judgement of multifunctional signal analysis processor 1.
Fig. 7 is the extension of electrochemical signal enlarger, it amplifies character deriving for Fig. 6 signal enlarger composition, mechanism derive by this can allow multiple biology sensor share this circuit the time, can rationally be amplified the usefulness of reinforcement electrochemical signals resolution when this detects on demand.In Fig. 6, an end points of a wiring 58 is connected in an amplifier 44 anodes, and another end points is connected to reference voltage end, so that the non-forward end of amplifier 44 has a voltage to level off to amplifier 44 anodes.One wiring 57 is connected in amplifier 44 negative terminals and sensor polarization end.The non-forward end of amplifier 44 is connected with resistance R n1 45 and change-over switch swn1 49 through wiring 57 and wiring 59, wiring 59 to 62 of wiring have a plurality of feedback resistance series connection, as Rn1 45～Rnn 48, wiring 59 to 62 of wiring have a plurality of change-over switch swn1 49～swnn 52 series connection.Amplifier 44 output terminals link to each other with resistance R nn 48 and change-over switch swnn 52 through wiring 62.Resistance R n1 45 is connected in change-over switch swn149 and change-over switch swn2 50 tie points with resistance R n2 46 tie points through wiring 60.Fixed resistance Rn2 46 is connected in change-over switch swn2 50 and change-over switch swn3 51 tie points with resistance R n3 47 tie points through wiring 60, and the rest may be inferred.Wiring 53 to wiring 56 is respectively the control line of swn1 49 to swnn 52.Different sensors can use not resistance and change-over switch on the same group respectively, opens a way as swn1.Simultaneously, when swn2 to swnn is all short circuit, then the impedance between Rn2 to Rnn is promptly little, can be considered Rn1 45 this moment and do the feedback amplification, amplify if do Rn2 46 feedbacks, with the swn2 open circuit, swn1 and swn3 to swnn are all short circuit simultaneously, the rest may be inferred, wherein Rn1, Rn2, Rn3 ... .Rnn and swn1, swn2, swn3 ... .swnn is extensible a plurality of.
Fig. 8 is for showing partly software flow pattern of a multifunctional signal analysis processor 1.After polarizing voltage that 1 all initialization of multifunctional signal analysis processor and power check and reference voltage unit 23 provide has checked or testing concentration measure finish back or the QC liquid measure of fixed concentration survey finish back or the inside value of reading store demonstration finish after or the signal analysis and processing device have any unusually after, signal analysis and processing device 1 promptly enters sleep state, and electric power controller 22 cuts out each device among Fig. 5.In step 64, signal analysis and processing device 1 is waken by concentration sensor or state detecting device.Then, judged whether that in step 65 external information card 6 inserts external information memory bank 2.If insertion arranged, then explanation is read the content in the external information internal memory 14 on the external information card 6 among the EEPROM 21 in the inlet signal analysis processor 1 in step 66.If no external information card 6 inserts, then can not read contents in the external information internal memory 14 and read to the interior EEPROM 21 of signal analysis and processing device 1.In step 67, signal analysis and processing device 1 is according to EEPROM21's " target determinand kind identification code " does the switching that detects which kind of determinand function, its change action is to change change-over switch 37 ON among Fig. 6 or the action of OFF, and the types in the EEPROM 21 are chosen parameter value and deposited in the rCOdeCardOption internal memory.
Then, step 68 expression obtains the resistance value of reading via concentration sensor in the concentration sensor slot 3 or state detecting device.Inspecting in the concentration sensor slot 3 according to the resistance condition is state detecting device or normal concentration sensor or bad concentration sensor 17.If the result who judges is improper concentration sensor, if then get back to sleep state according to the bad concentration sensor of resistance size condition judgment through step 69.If be judged as the state detecting device, then enter step 70.After step 70 expression signal analysis and processing device 1 is chosen the QC liquid pattern of fixed concentration, and get back to step 68 input end.
If judge the normal concentration sensor through step 68.Then to step 71.In step 71, environment temperature sensor 27 is obtained environment temperature, but and obtains calculated value to judge that environment temperature is whether in test specification.If environment temperature is normal, in step 72, after the QC liquid of fixed concentration or determinand splash in the concentration sensor 7 in the reaction zone 8, obtain its output voltage measuring value.If measuring value greater than the threshold values in the EEPROM 21, is then controlled reference voltage unit 23 by microprocessor 20 by bus 32, whether control the supplies of concentration sensor voltage by voltage cell 23, this section test duration is provided by the test duration parameter in the EEPROM 21.End when measuring, microprocessor 20 obtains concentration sensor reacting dose measured value.Enter step 73 this moment.In step 73, obtain the calculating parameter that to arrange in pairs or groups, through calculating the testing concentration value of reading.After the bound comparison of this value of reading in EEPROM 21, the value of reading or symbol are shown by display device 30.Then, enter sleep state at last.
Embodiment 1.
With blood sugar concentration sensor (ask for an interview TaiWan, China patent of invention card number 124332), by Portable multifunction electric chemical formula bio-detector of the present invention, carry out the analysis of whole blood blood sugar concentration, and be with reference to comparison method with YSI 2300 (electrochemical determination), it is splendid to determine that it detects effect, as shown in Figure 9.
Embodiment 2.
With uric acid concentration sensor (ask for an interview TaiWan, China patent of invention card number 107073), by Portable multifunction electric chemical formula bio-detector of the present invention, carry out the whole blood uric acid concentration analysis, and be with reference to comparison method with EPAC 6140 Biochemical Analyzers and Roche Uric Acid plus test group (ferment optical detecting method), it is splendid to determine that it detects effect, as shown in figure 10.
Embodiment 3.
With protoheme concentration sensor (asking for an interview TaiWan, China patent of invention notification number 466344), by Portable multifunction electric chemical formula bio-detector of the present invention, carry out whole blood protoheme concentration analysis, and be with reference to comparison method with Metertek SP-870 spectroanalysis instrument and SIGMA Hemoglobin test group (cyanbematin optical detecting method), it is good to determine that it detects effect, as Figure 11.
Embodiment 4.
The total cholesterol concentration sensor that is prepared from cholesterol oxidase, cholesterol esterase, surfactants and electronmediator, by Portable multifunction electric chemical formula bio-detector of the present invention, carry out the analysis of T-CHOL concentration of standard solution, and with the MetertekSP-870 spectroanalysis instrument with provide T-CHOL test group (ferment optical detecting method) for oneself for reference to comparison method, it is good to determine that it detects effect, as shown in figure 12.
Embodiment 5.
The lactic acid concn sensor that is prepared from lactate oxidase and electron mediator, by Portable multifunction electric chemical formula bio-detector of the present invention, carry out the lactic acid concn analysis of serum, with EPAC 6140 Biochemical Analyzers and SIGMA lactate test group (ferment optical detecting method) is with reference to comparison method, it is good to determine that it detects effect, as shown in figure 13.
Method of the present invention and feature will be more obvious through above-mentioned example explanation, but be it should be understood that any modification or change of being done under the spirit of the present invention that do not break away from, and all belong to protection scope of the present invention.
Claims (8)
1, a kind of Portable multifunction electric chemical formula bio-detector, this detector comprises:
A plurality of concentration sensors, each concentration sensor has a reaction zone, a reference electrode and a working electrode, wherein these electrodes are independent and continuous mutually, on this reaction zone, can put one can be corresponding with one the material of target determinand reaction, when detection reaction took place, working electrode can be exported an electrochemical reaction signal;
A plurality of external information internal memories, it corresponds respectively to this a plurality of concentration sensors, and each corresponding external information internal memory can provide the parameter information in order to the concentration of analyzing this corresponding target determinand;
One multifunctional signal analysis processor, it has a microprocessor, an one electronics erasable programmable read/write memory and an environment temperature sensor, this multifunctional signal analysis processor has two external input terminals at least, can link to each other with this external information internal memory with this concentration sensor respectively, this microprocessor all changes the parameter information in this external information internal memory over to earlier to this electronics erasable programmable read/write memory, making microprocessor only adopt internal information to carry out detects, and when electrochemical reaction, the parameter information that the electrochemical reaction signal that can export by this sensor and this electronics erasable programmable read/write memory are provided, and combination is in order to set up this temperature sensor of a temperature compensation, calculating the concentration of this corresponding target determinand, and with its testing result output;
Wherein this detector one group of concentration sensor of arranging in pairs or groups mutually of each use and external information internal memory detect the concentration of corresponding target determinand, can detect the concentration of different target determinand thus.
2, detector as claimed in claim 1, wherein this external information internal memory can be electronics erasable programmable read/write memory.
3, detector as claimed in claim 1, wherein the stored parameter of this external information internal memory comprises the detected parameters that activates threshold values, target determinand identification code, determination step, minute, temperature compensation, parameter checksum, computing method, uses when calculating a target testing concentration in order to this multifunctional signal analysis processor to be provided.
4, detector as claimed in claim 1, wherein this external information internal memory also comprises a parameter checksum, and calculate the data of this external information internal memory simultaneously and obtain the parameter summation by this microprocessor, with this parameter summation and this parameter checksum relatively, the data of judging this external information internal memory mistake whether.
5, detector as claimed in claim 1, wherein this sensor becomes reference electrode with an electrode grounding, another outside of serving as reasons provides a reference voltage to make the working electrode of its signal through amplifier and feedback resistance amplifying signal, electrochemical reaction signal with working electrode output when detecting offers this multifunctional signal analysis processor, calculates the concentration of target determinand.
6, detector as claimed in claim 1, this multifunctional signal analysis processor include one group of adjustable amplifier of multiplying power, and non-positive input is connected to this working electrode.
7, detector as claimed in claim 1, wherein
Provide a fixed voltage to working electrode by this microprocessor, and
After target determinand and reactant effect at the beginning, interrupt this voltage is provided, and after waiting for the detection stand-by period of appointment in this electronics erasable programmable read/write memory, provide a fixed voltage again to working electrode, to form the electrochemical reaction environment.
8, detector as claimed in claim 1, this detector further comprises:
One state detecting device, it includes two absolute electrodes, these absolute electrodes are connected by the resistance of fixed resistance value, this detector can link to each other with the concentration sensor link that is connected this multifunctional signal analysis processor, measure the resistance of this fixed resistance value by this multifunctional signal analysis processor, be built in the resistance of this processor in whether meeting, as the whether normal foundation of this multifunctional signal analysis processor state.
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| CNB021571848A CN1325906C (en) | 2002-12-16 | 2002-12-16 | Portable multifunctional electrochemical biological detecting instrument |
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| CNB021571848A CN1325906C (en) | 2002-12-16 | 2002-12-16 | Portable multifunctional electrochemical biological detecting instrument |
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| CN1508538A CN1508538A (en) | 2004-06-30 |
| CN1325906C true CN1325906C (en) | 2007-07-11 |
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| CNB021571848A Expired - Lifetime CN1325906C (en) | 2002-12-16 | 2002-12-16 | Portable multifunctional electrochemical biological detecting instrument |
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| TW559660B (en) * | 2002-06-21 | 2003-11-01 | Apex Biotechnology Corp | Portable multifunctional electrochemical bio-analyzer |
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| US8066866B2 (en) * | 2005-10-17 | 2011-11-29 | Lifescan, Inc. | Methods for measuring physiological fluids |
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| US8000918B2 (en) * | 2007-10-23 | 2011-08-16 | Edwards Lifesciences Corporation | Monitoring and compensating for temperature-related error in an electrochemical sensor |
| CN101470118A (en) * | 2007-12-24 | 2009-07-01 | 天津九安医疗电子股份有限公司 | Blood sugar detection apparatus |
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| CN103543185B (en) * | 2012-12-06 | 2015-06-24 | 理邦（美国）诊断有限公司 | Testing cartridge for an in vitro medical diagnostic device |
| CN104000601A (en) * | 2013-02-22 | 2014-08-27 | 谭唱 | Rapid blood uric acid measuring instrument |
| CN112603299A (en) * | 2020-11-20 | 2021-04-06 | 微泰医疗器械(杭州)股份有限公司 | Calibration method of implantable monitoring device, sensor assembly and blood glucose monitoring system |
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