CN105717289A - ELISA plate - Google Patents
ELISA plate Download PDFInfo
- Publication number
- CN105717289A CN105717289A CN201610173828.6A CN201610173828A CN105717289A CN 105717289 A CN105717289 A CN 105717289A CN 201610173828 A CN201610173828 A CN 201610173828A CN 105717289 A CN105717289 A CN 105717289A
- Authority
- CN
- China
- Prior art keywords
- micropore
- elisa plate
- protrusion
- wall
- reactive tank
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000002965 ELISA Methods 0.000 title claims abstract 16
- 239000000463 material Substances 0.000 claims description 5
- 239000011159 matrix material Substances 0.000 claims description 4
- 238000009826 distribution Methods 0.000 claims description 3
- 238000006243 chemical reaction Methods 0.000 abstract description 14
- 239000011148 porous material Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 description 8
- 238000001514 detection method Methods 0.000 description 6
- 239000007790 solid phase Substances 0.000 description 6
- 239000013076 target substance Substances 0.000 description 6
- 239000000427 antigen Substances 0.000 description 5
- 102000036639 antigens Human genes 0.000 description 5
- 108091007433 antigens Proteins 0.000 description 5
- 239000004793 Polystyrene Substances 0.000 description 4
- 230000002452 interceptive effect Effects 0.000 description 4
- 229920002223 polystyrene Polymers 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 238000006911 enzymatic reaction Methods 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 125000003636 chemical group Chemical group 0.000 description 1
- 238000010835 comparative analysis Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
Abstract
The invention discloses an ELISA plate. The ELISA plate can comprise a reaction tank and at least one microhole. Each microhole comprises a microhole outer wall and a protrusion, wherein the microhole outer wall is arranged on a bottom plate of the reaction tank, the protrusion is arranged in the microhole and connected with the bottom plate, and the protrusion is a conical tubular protrusion concentric with the microhole, or the protrusion is composed of a plurality of protruding blocks distributed in a conical tube shape and the conical tube formed by the protruding blocks is concentric with the microhole. The ELISA plate with the bottom of a concentric cone frustum structure with pores increases the reaction superficial area.
Description
Technical field
The present invention relates to the reaction enzymes field of immune detection in bioscience research field or medical diagnosis, particularly relate to a kind of ELISA Plate.
Background technology
Immune detection is the method for the modal detection target molecule of life science, and elisa (ELISA) is the important component part of immune detection.ELISA is broadly divided into two kinds of reactions: a kind of is immunoreation between antigen and antibody, and another kind is biomolecule and the solid phase surface adsorbed.
In elisa (ELISA), participate in the antigen of immunological response, antibody, traget antibody or the purity of antigen, concentration and ratio；Buffer kind, concentration and ionic strength, pH value and the condition such as reaction temperature, time play pivotal role.Additionally, the absorption of antigen, antibody or antigen antibody complex is also played very important effect by the ELISA Plate surface as carrier.
In prior art, the most frequently used material of ELISA ELISA Plate is polystyrene.In recent years, in order to increase the sensitivity of biomolecule and the affinity of solid phase surface, raising reaction, strengthening the stability etc. of detection, the selection of ELISA Plate solid phase material and process cause the very big of this area research worker and pay close attention to.At present, this area research worker is devoted to study the chemical property being modified the method such as polystyrene surface and ultraviolet irradiation change polystyrene surface by covalent cross-linking chemical group activating functional groups, chemical reaction more, and then improve the ELISA Plate hole surface affinity to biomolecule, and have been achieved for significant progress.
But, polystyrene ELISA Plate still suffers from the Surface area considerations in another problem and ELISA Plate hole and biomolecular reaction.ELISA Plate majority of the prior art is at the bottom of flat (structure is Figure of description 1 such as), the U-shaped end or V-type, flat refractive index is low, be suitable to microplate reader detection, the ELISA Plate refractive index at the U-shaped end is higher, facilitating application of sample, inhale the operation such as sample, mixing, the ELISA Plate at the bottom of V can pipette samples accurately.But the ELISA Plate hole of above-mentioned several ELISA Plate is less with the surface area of biomolecular reaction, it is unfavorable for fully quickly carrying out of reaction.
Summary of the invention
It is an object of the invention to provide a kind of ELISA Plate, the problem that the solid phase surface adsorbed to solve above-mentioned biomolecule amasss, make response area increase.
For achieving the above object, the invention provides following scheme: the application provides a kind of ELISA Plate, it is possible to including: reactive tank and at least one micropore；Described micropore includes micropore outer wall and protrusion, described micropore outer wall is placed on the base plate of described reactive tank, described protrusion is placed in described micropore, it is connected with described base plate, described protrusion is protruding with described micropore concentric tapered tubular, or the protruding block that described protrusion is distributed by multiple tapered tubulars is constituted, the conically shaped that the plurality of protruding block is formed is concentric with described micropore.
Optionally, described protrusion is made up of the protruding block often enclosing three tapered tubular distributions, is highly 0.2～5.0mm, and wall thickness is 0.1～1.0mm, has three holes between described three protruding blocks, and each width is 0.1～2.0mm.
Optionally, the conically shaped concentric structure that 3～5 circles are formed it is provided with by three described protrusions in described micropore, the diameter of the concentric tapered cylinder that described three protrusions are formed is different, respectively 0.2～2.0mm, 0.6～4.0mm, 1～6.0mm, 1.5～8.0mm, 2.0～10mm.All having hole, each width between any two protrusion in described three protrusions is 0.1～2.0mm.
Optionally, described micropore outer wall is higher than described protrusion.
Optionally, described micropore outer wall is divided into upper and lower two parts, and top is divided into cylindrical portion, and radius is 0.1～5.0mm, and wall thickness is 0.1～1.0mm, is highly 0.1～20mm；Bottom is divided into infundibulum wide at the top and narrow at the bottom, and the radius of the upper shed of described infundibulum is identical with the radius of described cylindrical portion, is 0.1～5.0mm, and lower port radius is 0.1～4.0mm, and wall thickness is 0.1～1.0mm.The height of infundibulum is 0.2～6.0mm.The under shed of described infundibulum is connected with described reactive tank base plate.
Optionally, described micropore is that matrix array is uniformly arranged on described reactive tank base plate, is 1～500.Optionally, each lateral wall of described micropore and the upright projection of described reactive tank base plate are all non-intersect with adjacent cells.
Optionally, described reactive tank includes base plate and sidewall, and the relatively described sidewall in described reactive tank base plate four limit stretches out a part.
Optionally, described protrusion is made up of transparent or semitransparent material.
Optionally, described ELISA Plate, it is characterised in that the area of ELISA Plate is 10～200 × 5～200mm2 ELISA Plate, each ELISA Plate includes 1～500 ELISA Plate micropore.
The present invention arranges the purpose of protrusion and is in that to increase the surface area of micropore, increases the chance that target substance contact with the target molecule of solid phase surface, while can use less sample, detect the content of high concentration target substance.
Hole of the present invention is arranged on the wall of tapered protrusion thing, and described hole runs through the longitudinal section of whole protrusion.Therefore, the hole that the present invention is arranged can make liquid in the internal free-flow of micropore, makes target substance and target molecule fully react.
The infundibulum of micropore bottom of the present invention, infundibulum is bottom micropore and the buffer unit on top.
Protrusion wall of the present invention is the hollow tubular structure having certain altitude and thickness, protrudes wall and is possible to prevent interfering between micropore.
According to specific embodiment provided by the invention, the invention discloses techniques below effect: a kind of bottom of present invention protection is the ELISA Plate with hole concentric circular platform structure, adds the surface area of reaction, reduce interfering between micropore simultaneously.
Accompanying drawing explanation
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, the accompanying drawing used required in embodiment will be briefly described below, apparently, accompanying drawing in the following describes is only some embodiments of the present invention, for those of ordinary skill in the art, under the premise not paying creative work, it is also possible to obtain other accompanying drawing according to these accompanying drawings；
Fig. 1 is the axonometric chart of tradition ELISA Plate；
Fig. 2 is top view (Fig. 2 A) and the front view (Fig. 2 B) of the microcellular structure of the present invention；
Fig. 3 is the axonometric chart of the microcellular structure of the present invention；
Fig. 4 A-1 is the top view of 20 hole ELISA Plate of 4 × 5 rectangular array of the present invention；
Fig. 4 A-2 is the side view of 20 hole ELISA Plate of 4 × 5 rectangular array of the present invention；
Fig. 4 B-1 is the top view of 28 hole ELISA Plate of 4 × 7 rectangular array of the present invention；
Fig. 4 B-2 is the side view of 28 hole ELISA Plate of 4 × 7 rectangular array of the present invention；
Fig. 5 is the a-a sectional view of 28 hole ELISA Plate of 20 hole ELISA Plate of 4 × 5 rectangular array of the present invention or 4 × 7 rectangular array；
Fig. 6 A and Fig. 6 B is the axonometric chart of 28 hole ELISA Plate of 20 hole ELISA Plate of 4 × 5 rectangular array of the present invention and 4 × 7 rectangular array respectively；
1-micropore；2-micropore outer wall；3-protrusion；4-cylindrical portion；5-infundibulum；6-protruding block；7-hole；8-reactive tank base plate；9-reactive tank sidewall.
Detailed description of the invention
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described, it is clear that described embodiment is only a part of embodiment of the present invention, rather than whole embodiments.Based on the embodiment in the present invention, the every other embodiment that those of ordinary skill in the art obtain under not making creative work premise, broadly fall into the scope of protection of the invention.
It is an object of the invention to provide a kind of ELISA Plate, to solve above-mentioned prior art Problems existing, make response area increase.
Understandable for enabling the above-mentioned purpose of the present invention, feature and advantage to become apparent from, below in conjunction with the drawings and specific embodiments, the present invention is further detailed explanation.
The application provides a kind of ELISA Plate, it is possible to include structure as shown in Figure 3: reactive tank and at least one micropore 1；Described micropore 1 includes micropore outer wall 2 and protrusion 3, described micropore outer wall 2 is placed on the base plate of described reactive tank, described protrusion 3 is placed in described micropore, it is connected with described base plate, described protrusion 3 is protruding with described micropore concentric tapered tubular, or the protruding block 6 that described protrusion is distributed by multiple tapered tubulars is constituted, the conically shaped that the plurality of protruding block 6 is formed is concentric with described micropore.
Optionally, the protruding block 6 that described protrusion 3 is distributed by three tapered tubulars is constituted, and has three holes 7 between described three protruding blocks 6.
Optionally, it is provided with 3～5 conically shaped concentric structures that formed by three described protrusions of circle in described micropore 1 to form, the conically shaped that described three protrusions are formed with one heart and the diameter of the conically shaped formed different, respectively 0.2～2.0mm, 0.6～4.0mm, 1～6.0mm, 1.5～8.0mm, 2.0～10mm, being highly 0.2～5.0mm, wall thickness is 0.1～1.0mm.In described three protrusions, the hole 7 of arbitrary protrusion 3 is all relative with the middle part of the protruding block 6 of adjacent protrusion thing 3.
Optionally, described micropore outer wall is higher than described protrusion.
Optionally, described micropore outer wall is divided into upper and lower two parts, and top is divided into cylindrical portion 4, and radius is 0.1～5.0mm, and wall thickness is 0.1～1.0mm, is highly 0.1～20mm；Bottom is divided into infundibulum 5 wide at the top and narrow at the bottom, and the radius of the upper shed of described infundibulum is identical with the radius of described cylindrical portion, is 0.1～5.0mm, and lower port radius is 0.1～4.0mm, and wall thickness is 0.1～1.0mm.The height of infundibulum is 0.2～6.0mm.The under shed of described infundibulum is connected with described reactive tank base plate 8.
Optionally, described micropore 1 is uniformly arranged on described reactive tank base plate 8 in matrix array.
Optionally, each lateral wall of described micropore 1 and the upright projection of described reactive tank base plate 8 are all non-intersect with adjacent cells.
Optionally, described protrusion is made up of transparent or semitransparent material.
Optionally, described ELISA Plate, it is characterised in that the area of ELISA Plate is 10～200 × 5～200mm2 ELISA Plate, each ELISA Plate includes 1～500 ELISA Plate micropore.
Below in conjunction with embodiment, ELISA Plate provided by the invention is described in detail, but they can not be interpreted as limiting the scope of the present invention.
Embodiment 1
As shown in Figures 2 and 3, present embodiments providing a kind of microcellular structure formed with the protrusion of hole, the infundibulum of micropore bottom and protrusion three, the wall part on micropore top within micropore, this microcellular structure is a kind of microcellular structure that can increase reaction table area.
Embodiment 2
As in figure 2 it is shown, the outer wall of each taper tubular protrusion is provided with equally distributed three inwall arc length L1The hole of=0.5mm, hole runs through the whole longitudinal section of taper tubular protrusion.Hole can allow liquid in the internal free-flow of micropore.
Embodiment 3
As shown in Figure 5, Figure 6, this example provides 20 hole ELISA Plate of 4 × 5 rectangular array and 28 hole ELISA Plate of 4 × 7 rectangular array, and except the matrix arrangement difference of micropore, 20 hole ELISA Plate are identical with the microcellular structure of 28 hole ELISA Plate.
Embodiment 4
As shown in Figure 5, it is provided that the diameter of 20 hole ELISA Plate of 4 × 5 rectangular array and the taper tubular protrusion bottom 28 hole ELISA Plate micropores of 4 × 7 rectangular array is respectively set to φ1=4.0mm, φ2=3.0mm, φ3=2.0mm and φ4=1.0mm, outermost taper tubular protrusion thickness is set to δ 1=0.2mm, the thickness of other taper tubular protrusions is disposed as δ2=0.15mm, the height of taper tubular protrusion is disposed as h1=0.8mm, taper tubular protrusion is set to α=67.38 ° with the angle at the bottom of ELISA Plate plate.
The infundibulum of micropore bottom is hollow round table-like structure, and the height of infundibulum is 1/2nd of taper tubular protrusion height, i.e. h2=0.4mm.
The protrusion wall on micropore top is tubular structure, and the height protruding wall is set to 5/4ths of micropore bottom level, i.e. h3=1.0mm.
The microcellular structure surface area computing formula of embodiment 4:
The surface area of the flat micropore of tradition
The surface area of embodiment 4 micropore medial wall and
The surface area reduced of the hole of embodiment 4 taper tubular protrusion sidewall
Embodiment 4 micropore lateral wall be perpendicular at the bottom of ELISA Plate plate projection surface area and
The surface area that embodiment 4 micropore is total
The surface area S that embodiment 4 micropore is totalAlwaysWith conventional microporous surface area SFlat microporeRatio be
The volume calculations formula of micropore described in embodiment 4:
The volume of outermost micropore
The volume of other taper tubular protrusions
The volume of hole
The volume of micropore
Embodiment 5
As shown in Fig. 4 A-1 and Fig. 4 B-1, the enzyme mark version of present invention protection, described array distribution micropore on reactive tank base plate is sequentially carried out numeral numbering, string micropore label respectively "-" in the middle of wherein, "+", " ++ " and " +++ ".Micropore is numbered, facilitates the observation of enzyme reaction, record, it is possible to the difference of enzyme reaction in clearly comparative analysis difference numbering micropore.
The present invention arranges the purpose of tapered protrusion thing and is in that to increase the surface area of micropore, increases the chance that target substance contact with the target molecule of solid phase surface, while can use less sample, detect the content of high concentration target substance.
Hole of the present invention is arranged on the wall of tapered protrusion thing, and described hole runs through the longitudinal section of whole concentric round platform.Therefore, the hole that the present invention is arranged can make liquid in the internal free-flow of micropore, makes target substance and target molecule fully react.
The infundibulum of micropore bottom of the present invention, infundibulum is bottom micropore and the buffer unit on top.
Protrusion wall of the present invention is the hollow tubular structure having certain altitude and thickness, protrudes wall and is possible to prevent interfering between micropore.
According to specific embodiment provided by the invention, the invention discloses techniques below effect: a kind of bottom of present invention protection is the ELISA Plate with hole concentric circular platform structure, adds the surface area of reaction, reduce interfering between micropore simultaneously.
In this specification, each embodiment adopts the mode gone forward one by one to describe, and what each embodiment stressed is the difference with other embodiments, between each embodiment identical similar portion mutually referring to.For system disclosed in embodiment, owing to it corresponds to the method disclosed in Example, so what describe is fairly simple, relevant part illustrates referring to method part.
Principles of the invention and embodiment are set forth by specific case used herein, and the explanation of above example is only intended to help to understand method and the core concept thereof of the present invention；Simultaneously for one of ordinary skill in the art, according to the thought of the present invention, all will change in specific embodiments and applications.In sum, this specification content should not be construed as limitation of the present invention.
Claims (10)
1. an ELISA Plate, it is characterised in that: including: reactive tank and at least one micropore (1)；Described micropore (1) includes micropore outer wall (2) and protrusion (3), described micropore outer wall (2) is placed on the base plate (8) of described reactive tank, described protrusion (3) is placed in described micropore, it is connected with described base plate, described protrusion (3) is protruding with described micropore concentric tapered tubular, or the protruding block that described protrusion (3) is distributed by multiple tapered tubulars is constituted, the conically shaped that the plurality of protruding block (6) is formed is concentric with described micropore.
2. a kind of ELISA Plate according to claim 1, it is characterized in that: described protrusion (3) is made up of the protruding block (6) often enclosing three tapered tubular distributions, it is highly 0.2～5.0mm, wall thickness is 0.1～1.0mm, having three holes (7) between described three protruding blocks (6), each width is 0.1～2.0mm.
3. a kind of ELISA Plate according to claim 2, it is characterized in that: be provided with 3～5 conically shaped concentric structures that formed by three described protrusions of circle in described micropore (1) and form (3), the conically shaped that described three protrusions (3) are formed with one heart and the diameter of the conically shaped formed different, respectively 0.2～2.0mm, 0.6～4.0mm, 1～6.0mm, 1.5～8.0mm, 2.0～10mm.All having hole (7) between any two protrusion in described three protrusions (3), each width is 0.1～2.0mm.
4. a kind of ELISA Plate according to claim 1, it is characterised in that: described micropore outer wall is higher than described protrusion.
5. a kind of ELISA Plate according to claim 1, it is characterised in that: described micropore outer wall is divided into upper and lower two parts, and top is divided into cylindrical portion (4), and radius is 0.1～5.0mm, and wall thickness is 0.1～1.0mm, is highly 0.1～20mm；Bottom is divided into infundibulum (5) wide at the top and narrow at the bottom, and the radius of the upper shed of described infundibulum (5) is identical with the radius of described cylindrical portion (4), is 0.1～5.0mm, and lower port radius is 0.1～4.0mm, and wall thickness is 0.1～1.0mm.The height of infundibulum is 0.2～6.0mm.The under shed of described infundibulum (5) is connected with described reactive tank base plate (8).
6. a kind of ELISA Plate according to claim 1, it is characterised in that: described micropore (1) is uniformly arranged in matrix array on described reactive tank base plate (8), is 1～500.
7. a kind of ELISA Plate according to claim 1, it is characterised in that: each lateral wall of described micropore (1) and the upright projection of described reactive tank base plate (8) are all non-intersect with adjacent cells.
8. a kind of ELISA Plate according to claim 1, it is characterized in that: described reactive tank includes base plate (8) and sidewall (9), the relatively described sidewall (9) in described reactive tank base plate (8) four limit stretches out a part.
9. ELISA Plate according to any one of claim 1 to 8, it is characterised in that described protrusion is made up of transparent or semitransparent material.
10. ELISA Plate according to claim 1, it is characterised in that the area of ELISA Plate is 10～200 × 5～200mm2ELISA Plate, each ELISA Plate includes 1～500 ELISA Plate micropore.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610173828.6A CN105717289B (en) | 2016-03-24 | 2016-03-24 | A kind of ELISA Plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610173828.6A CN105717289B (en) | 2016-03-24 | 2016-03-24 | A kind of ELISA Plate |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105717289A true CN105717289A (en) | 2016-06-29 |
| CN105717289B CN105717289B (en) | 2017-11-24 |
Family
ID=56159229
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610173828.6A Active CN105717289B (en) | 2016-03-24 | 2016-03-24 | A kind of ELISA Plate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105717289B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018006466A1 (en) * | 2016-07-08 | 2018-01-11 | 沈阳医学院 | Freely-assembled enzyme label plate |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5679310A (en) * | 1995-07-11 | 1997-10-21 | Polyfiltronics, Inc. | High surface area multiwell test plate |
| CN2760557Y (en) * | 2004-12-21 | 2006-02-22 | 上海荣盛生物技术有限公司 | Elisa plate with adsorptive force |
| CN101533009A (en) * | 2009-04-15 | 2009-09-16 | 徐恩良 | Micropore structure of microplate strip |
| CN202693587U (en) * | 2012-08-13 | 2013-01-23 | 沃克(天津)生物科技有限公司 | Reaction plate without measuring light transmittance |
| CN104977405A (en) * | 2015-07-13 | 2015-10-14 | 徐恩良 | Immunodetection micropore |
| CN205506833U (en) * | 2016-03-24 | 2016-08-24 | 何韶衡 | Elisa plate |
-
2016
- 2016-03-24 CN CN201610173828.6A patent/CN105717289B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5679310A (en) * | 1995-07-11 | 1997-10-21 | Polyfiltronics, Inc. | High surface area multiwell test plate |
| CN2760557Y (en) * | 2004-12-21 | 2006-02-22 | 上海荣盛生物技术有限公司 | Elisa plate with adsorptive force |
| CN101533009A (en) * | 2009-04-15 | 2009-09-16 | 徐恩良 | Micropore structure of microplate strip |
| CN202693587U (en) * | 2012-08-13 | 2013-01-23 | 沃克(天津)生物科技有限公司 | Reaction plate without measuring light transmittance |
| CN104977405A (en) * | 2015-07-13 | 2015-10-14 | 徐恩良 | Immunodetection micropore |
| CN205506833U (en) * | 2016-03-24 | 2016-08-24 | 何韶衡 | Elisa plate |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018006466A1 (en) * | 2016-07-08 | 2018-01-11 | 沈阳医学院 | Freely-assembled enzyme label plate |
| US10507467B2 (en) | 2016-07-08 | 2019-12-17 | Shenyang Medical College | Freely-assembled ELISA plate |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105717289B (en) | 2017-11-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR20150047598A (en) | Microwell plate | |
| KR101167435B1 (en) | Cell Chip | |
| CN105699644B (en) | A kind of ELISA Plate | |
| CN102698672B (en) | Microfluidic reactor | |
| CN105717289A (en) | ELISA plate | |
| CN108226249B (en) | Disposable nanopore biosensor and manufacturing method thereof | |
| CN205506833U (en) | Elisa plate | |
| CN206838154U (en) | A kind of clinical laboratory inspection test tube rack | |
| CN205965943U (en) | Combined multifunctional test -tube frame | |
| CN202013353U (en) | Elisa plate with detachable elisa hole | |
| CN111826284A (en) | High-flux culture plate, high-flux multi-organ co-culture chip and application thereof | |
| CN202189053U (en) | Biological chip | |
| CN207318405U (en) | A kind of nano-pore detection device of modified biological probe | |
| CN1661039A (en) | Method for preparing chips of cell and equipment | |
| CN201192640Y (en) | Weighing boat special for experimental laboratory | |
| CN203960220U (en) | For the needle cover of paramagnetic particle method | |
| CN214122123U (en) | Detection device for double derivation of single-column post-derivation liquid chromatograph | |
| CN2554176Y (en) | Bio-chip for detecting rheumatic disease index | |
| CN107486269A (en) | The classifiable tumor markers in detecting micro flow control chip device that women is applicable | |
| Xiao et al. | Single‐Cell Enzymatic Screening for Epithelial Mesenchymal Transition with an Ultrasensitive Superwetting Droplet‐Array Microchip | |
| CN107340393A (en) | Six kinds of classifiable tumor mark multichannels while detection micro flow control chip device | |
| CN107335485A (en) | General type is used for the micro flow control chip device for detecting Diagnostic Value of Several Serum Tumor Markers simultaneously | |
| CN107335487A (en) | The classifiable tumor markers in detecting micro flow control chip device that women is applicable | |
| CN107754891A (en) | The micro flow control chip device of a variety of classifiable tumor marks of women is detected simultaneously | |
| CN107486266A (en) | The classifiable tumor markers in detecting micro flow control chip device that male is applicable |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210310 Address after: 110027 no.36-3, Huahai Road, Shenyang Economic and Technological Development Zone, Liaoning Province Patentee after: SHENYANG HUIMINYUAN BIOTECHNOLOGY Co.,Ltd. Address before: 121000 35-97, jinxiutiandi District D, Lingnan Xili, Taihe District, Jinzhou City, Liaoning Province Patentee before: He Shaoheng |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A type of enzyme-linked immunosorbent assay plate Effective date of registration: 20231225 Granted publication date: 20171124 Pledgee: Shenyang Science and Technology Venture Capital Co.,Ltd. Pledgor: SHENYANG HUIMINYUAN BIOTECHNOLOGY Co.,Ltd. Registration number: Y2023210000355 |