CN105606573A - Rapid intraoperative pathological diagnosis system and method - Google Patents
Rapid intraoperative pathological diagnosis system and method Download PDFInfo
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- CN105606573A CN105606573A CN201510967267.2A CN201510967267A CN105606573A CN 105606573 A CN105606573 A CN 105606573A CN 201510967267 A CN201510967267 A CN 201510967267A CN 105606573 A CN105606573 A CN 105606573A
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
Abstract
The invention is suitable for the technical field of medical information and provides a rapid intraoperative pathological diagnosis system and method. The system comprises a sample preparation module, an optical imaging module and a data analysis module, wherein the sample preparation module extracts a diseased sample tissue from a patient body, fixes the diseased sample tissue by adopting a chemical reagent, then performs optical clearing treatment on the diseased sample tissue by adopting an optical clearing agent and finally performs fluorescent staining treatment on the diseased sample tissue; the optical imaging module first arranges and fixes the diseased sample tissue subjected to the fluorescent staining treatment, and then performs optical scanning imaging treatment on the diseased sample tissue subjected to the fluorescent staining treatment, by adopting a functional microimaging system, so as to obtain a functional diagram and an optical section diagram of the diseased sample tissue; and the data analysis module performs comparative analysis on the functional diagram and the optical section diagram as well as existing disease database data to generate a diagnosis result report. The system and the method are high in section-making speed and high in section-making quality.
Description
Technical field
The invention belongs to medical information technical field, the system that relates in particular to the diagnosis of a kind of early diagnosis withMethod.
Background technology
Pathological diagnosis is the diagnosis of tool authority in current modern medicine, and it refers to the need of answering Clinics and PracticesWant, in patient body, take out pathological tissues, carry out the technology of pathologic finding. By to pathological tissues and cellThe analysis of form, identification, then in conjunction with visually observing and clinical related data, make the diagnosis of various diseases.
Pathological diagnosis in art, short because of its required time, in art, just can determine that pathology is good pernicious, according to diagnosis knotFruit can, for the further operation plan of clinical formulation provides foundation, be subject to the clinical hand of domestic and international various big hospital day by dayThe welcome of subject chamber. In art, pathological diagnosis is made a definite diagnosis the not clear pathology of character in operation is carried out,If be diagnosed as malignant tumour, just can directly select modus operandi and scope according to result, optimize operation sideCase, thus second operation and damage effectively avoided. Sick in the art that various big hospital generally uses both at home and abroad at presentReason diagnostic method mainly contains three kinds: quick frozen-section pathologic finding in (1) art, it is by excision in operationPathological tissue fast film-making under freezing state, after conventional rapid dyeing, carry out fast for Pathology Doctors 'Pathological diagnosis, then will report the result issue clinical. Pathology Doctors ' is generally being received about sample 30-50 minuteMake the quick diagnosis of sample. But it has certain limitation in application, and tissue can not be too small, can notMass simultaneous is provided pathological diagnosis result, and ice crystal easily appears in frozen section, and cellular swelling distortion etc. are existingResemble. (2) routine paraffin wax section pathologic finding, applied range, is pathology excellent diagnostics, but the film-making timeVery long, making pathological diagnosis needs 3-5 days, can not meet clinical and patient far away to early diagnosing early treatmentDemand. (3) quick paraffin sections pathologic finding, it is by high-temperature heating, allows sample be organized in the short timeInside complete fast dewatering and waxdip process, its principle is similar to routine paraffin wax section pathologic finding to effect. RootAccording to drawing materials, the factor such as the diagnostic level of production effect and Pathology Doctors ', its needs 50 minutes-24 hours sidesCan send report. Although chipping qualities is cut into slices not second to routine paraffin wax, cuts owing to having simplified routine paraffin waxThe manufacturing process of sheet, its slice-making quality is difficult to control.
Summary of the invention
In view of this, the embodiment of the present invention provides a kind of System and method for of early diagnosis diagnosis, to separateThe method of pathological diagnosis in the art that certainly prior art provides, film-making speed is slow and slice-making quality is very rambunctiousProblem.
First aspect, the system that provides a kind of early diagnosis to diagnose, is characterized in that described system bagDraw together: sample is prepared module, optical imagery module and data analysis module;
Described sample is prepared module and is comprised:
Tissue collecting unit, for extracting pathology sample tissue in patient body;
Organize fixed cell, for the pathology sample that adopts the fixing described tissue collecting of chemical reagent unit to extractTissue;
Organize optical transparency unit, for adopting optical transparency agent to the described fixing pathology sample of fixed cell of organizingTissue carries out optical transparency processing;
Tissue staining unit, for carrying out to the described optical transparency unit of organizing the pathology sample that optical transparency processing obtainsThis tissue carries out fluorescent staining processing;
Described optical imagery module comprises:
Sample placement unit, for placing and fixing described tissue staining unit and carry out fluorescent staining processing and obtainPathology sample tissue;
Scanning imagery unit, for adopting functional form micro imaging system to carry out glimmering to described tissue staining unitLight dyeing processes the pathology sample tissue obtaining and carries out optical scanner imaging, obtains pathology sample tissueFunctional diagram and optical section figure;
Described data analysis module, the functional diagram that described scanning imagery unit is obtained and optical section figure are with existingThere is illness database data to be analyzed, generate diagnostic result report.
Further, the described optical transparency unit of organizing is to the described fixing pathology sample tissue of fixed cell of organizingCarrying out optical transparency processing is specially: the refractive index changing in pathology sample tissue is not mated, and increases light in pathologyPenetration power in sample tissue.
Further, described scanning imagery unit comprises:
The first imaging subelement, for by functional form micro imaging system to pathology sample tissue horizontal twoDimension scanning obtains the functional diagram of pathology sample tissue;
The second imaging subelement, for longitudinally the sweeping pathology sample tissue by functional form micro imaging systemRetouch the optical section figure that obtains pathology sample tissue.
Further, described scanning imagery unit also comprises:
Image storage and display unit, for storing and showing described the first imaging subelement and the second imaging singlyFunctional diagram and the optical section figure of the pathology sample tissue that unit's scanning obtains;
Graphics processing unit, for the functional diagram to the storage of described image storage and display unit and optical section figureCarry out digital processing.
Further, described data analysis module comprises:
Pathogeny analytic unit, sweeps for the illness data and the described optical imagery module that contrast existing databaseRetouch the functional diagram and the optical section figure that obtain, infer disease source and evolutionary process;
Lesion nature analytic unit, for determining and analyzing lesion nature;
Diagnostic analysis unit, for carrying out medical diagnosis on disease analysis, provides diagnostic comments;
Report generation unit, for generating and sending diagnosis report.
Second aspect, a kind of method that provides early diagnosis to diagnose, described method comprises:
In patient body, extract pathology sample tissue;
Adopt the fixing described pathology sample tissue of chemical reagent;
Adopt optical transparency agent to carry out optical transparency processing to fixing described pathology sample tissue;
Carry out fluorescent staining processing to carrying out the pathology sample tissue that optical transparency processing obtains;
Place and fixedly carry out the pathology sample tissue that fluorescent staining processing obtains;
Adopt functional form micro imaging system to carry out light to the pathology sample tissue that carries out fluorescent staining processing and obtainLearn scanning imagery processing, obtain functional diagram and the optical section figure of pathology sample tissue;
It is right that the functional diagram that scanning imagery processing is obtained and optical section figure and existing illness database data carry outThan analyzing, generate diagnostic result report.
Further, the agent of described employing optical transparency is carried out optical transparency processing to fixing described pathology sample tissueBe specially: the refractive index changing in pathology sample tissue is not mated, and increases light wearing in pathology sample tissuePower thoroughly.
Further, the pathology that described employing functional form micro imaging system obtains carrying out fluorescent staining processingSample tissue carries out optical scanner imaging, obtains functional diagram and the optical section figure of pathology sample tissue,Comprise:
By functional form micro imaging system, the horizontal two-dimensional scan of pathology sample tissue is obtained to pathology sample groupThe functional diagram of knitting;
By functional form micro imaging system, the longitudinal scanning of pathology sample tissue is obtained to pathology sample tissueOptical section figure.
Further, the disease obtaining carrying out fluorescent staining processing at described employing functional form micro imaging systemThis tissue that changes carries out optical scanner imaging, obtains functional diagram and the optical section figure of pathology sample tissueAfterwards, also comprise:
Functional diagram and the optical section figure of the pathology sample tissue that storage reading scan obtain;
Described functional diagram and optical section figure are carried out to digital processing.
Further, the described functional diagram that scanning imagery processing is obtained and optical section figure and existing illness numberBe analyzed according to database data, generate diagnostic result report, comprising:
Illness data and described optical imagery module in contrast existing database scan the functional diagram and the light that obtainLearn slice map, infer disease source and evolutionary process;
Determine and analyze lesion nature;
Carry out medical diagnosis on disease analysis, provide diagnostic comments;
Generate and send diagnosis report.
In the embodiment of the present invention, by sample prepare module to pathology sample tissue be fixed, optical transparency,After fluorescent staining is processed, then by optical imagery module to sample prepare pathology sample prepared by module organize intoRow optical imagery scans, and can obtain functional diagram and the optical section figure of pathology sample tissue. This by drawingThe mode that light inlet transparent technology combines with optical image technology, can realize the optics of similar actual slice and cutSheet function, has further avoided the shared time of actual slice. And, owing to there not being actual slice,Pathology sample tissue morphology and structure are preserved complete, and optical imagery effect definition can be cut into slices with routine paraffin waxThe effect of pathologic finding compares favourably, for doctor provides diagnostic data comparatively accurately. In addition, with current applicationIn maximum arts, pathologic finding-quick frozen-section pathologic finding is compared, fast in a kind of art provided by the inventionSpeed pathological diagnosis system, can realize the quick diagnosis of 60 minutes in theory the soonest.
Brief description of the drawings
Fig. 1 is the structured flowchart of the system embodiment of early diagnosis diagnosis of the present invention;
Fig. 2 is the realization flow figure of the embodiment of the method for early diagnosis diagnosis of the present invention.
Detailed description of the invention
In order to make object of the present invention, technical scheme and advantage clearer, below in conjunction with accompanying drawing and realityExecute example, the present invention is further elaborated. Only should be appreciated that specific embodiment described hereinOnly, in order to explain the present invention, be not intended to limit the present invention.
The system of the early diagnosis diagnosis providing in embodiments of the present invention, comprises: sample is prepared mouldPiece, optical imagery module and data analysis module; Described sample is prepared module and is comprised: tissue collecting unit,For extract pathology sample tissue in patient body; Organize fixed cell, for adopting chemical reagent to fix instituteState the pathology sample tissue that tissue collecting unit extracts; Organize optical transparency unit, for adopting optical transparency agent pairThe described fixing pathology sample tissue of fixed cell of organizing carries out optical transparency processing; Tissue staining unit, forOrganize optical transparency unit to carry out the pathology sample tissue that optical transparency processing obtains carry out fluorescent staining place to describedReason; Described optical imagery module comprises: sample placement unit, and for placing and fix described tissue staining listUnit carries out the pathology sample tissue that fluorescent staining processing obtains; Scanning imagery unit, for adopting functional form aobviousMicro-imaging system is carried out to described tissue staining unit the pathology sample tissue that fluorescent staining processing obtains and is carried out lightLearn scanning imagery processing, obtain functional diagram and the optical section figure of pathology sample tissue; Described data analysis mouldPiece, the functional diagram that described scanning imagery unit is obtained and optical section figure and existing illness database data enterRow comparative analysis, generates diagnostic result report.
Below in conjunction with specific embodiment, realization of the present invention is described in detail:
Embodiment mono-
Fig. 1 show the early diagnosis diagnosis that the embodiment of the present invention one provides system concrete structureBlock diagram, for convenience of explanation, only shows the part relevant to the embodiment of the present invention. This early diagnosisThe system 1 of diagnosis comprises: sample is prepared module 11, optical imagery module 12 and data analysis module 13;
Wherein, described sample is prepared module 11 and is comprised:
Tissue collecting unit 111, for extracting pathology sample tissue in patient body;
Organize fixed cell 112, for the pathology that adopts the fixing described tissue collecting of chemical reagent unit to extractSample tissue;
Organize optical transparency unit 113, for adopting optical transparency agent to the described fixing pathology of fixed cell of organizingSample tissue carries out optical transparency processing;
Tissue staining unit 114, for carrying out to the described optical transparency unit of organizing the disease that optical transparency processing obtainsThis tissue that changes carries out fluorescent staining processing.
Concrete, tissue collecting unit 111 can extract pathology sample tissue in patient body, for follow-up rightPathology sample tissue treatment provides must material. The operation of extracting includes but not limited to cut, clamp get, pierce through,Peel off etc.
Fixing pathology sample tissue refers to the component of solidifying rapidly in pathology sample tissue, and keeps it to liveStructure when body, organizes after the fixing pathology sample tissue of fixed cell 112, can be conducive to follow-up to pathologyThe optical transparency of sample tissue is processed and fluorescent staining processing. The chemical reagent of fixing pathology sample tissue comprise butBe not limited to formalin, formaldehyde, phosphate buffers etc., are controlled at 10 to the fixing used time of pathology sample tissueAbout minute.
Optical transparency processing refers to that the refractive index changing in pathology sample tissue do not mate, and increase light is at pathology samplePenetration power in tissue, carries out, after optical transparency processing, can facilitating subsequent optical imaging to pathology sample tissueModule 12 is carried out the operation of profound optical section to pathology sample tissue. After this pathology sample tissue is without againComplex operations while carrying out similar making paraffin section, has reduced the time that sample is made greatly. To differencePathology sample tissue can specific aim adopt the clarifier of different formulations, different proportionings, as number of patent applicationClarifier in CN104568553A, according to the situation of pathology sample tissue, can suitably change fructose, poly-The ratio of ethylene glycol, urea, glycerine etc. The optical transparency processing used time to pathology sample tissue is controlled at 10About minute.
To the fluorescent staining processing of pathology sample tissue, can facilitate subsequent optical image-forming module 12 to pathology sampleThis tissue carries out the operation of optical scanner imaging. When the selection of fluorescent dye can be according to subsequent optical imaging, selectThe micro imaging system of selecting and determining, staining method includes but not limited to fluorescence immunization coloration, fluorescent antibody stainingDeng. The fluorescent staining processing used time to pathology sample tissue was controlled at about 10 minutes.
Described optical imagery module 12 comprises:
Sample placement unit 121, for placing and fixing described tissue staining unit and carry out fluorescent staining processingThe pathology sample tissue obtaining;
Scanning imagery unit 122, for adopting functional form micro imaging system to enter described tissue staining unitThe pathology sample tissue that row fluorescent staining processing obtains carries out optical scanner imaging, obtains pathology sample groupThe functional diagram of knitting and optical section figure.
Wherein, functional form micro imaging system includes but not limited to that confocal microscopic image system, two-photon showMicro-imaging system, mating plate formula illumination micro imaging system, Structured Illumination micro imaging system etc.
Concrete, sample placement unit 121 can be anchored on pathology sample tissue functional form micro-imaging systemOn the objective table of system, to guarantee that, in the process of subsequent optical imaging, pathology sample is not organized and can be moved.Wherein, the firm mode of sample includes but not limited to screw locking, spring leaf top pressure etc.
Scanning imagery unit 122 comprises:
The first imaging subelement, for by functional form micro imaging system to pathology sample tissue horizontal twoDimension scanning obtains the functional diagram of pathology sample tissue;
The second imaging subelement, for longitudinally the sweeping pathology sample tissue by functional form micro imaging systemRetouch the optical section figure that obtains pathology sample tissue.
Wherein, the selection of functional form micro imaging system determines that the mode of transversal scanning includes but not limited to structureOptical illumination surface imaging, line by line scan, spiral scan etc., the precision of longitudinal scanning is according to pathology sample tissueThickness and the demand of slice thickness can select in 0.5 μ m-5 μ m fluctuation. Optics to pathology sample tissue is sweptRetouching the imaging used time was controlled at about 10 minutes.
Preferably, described optical imagery module 12 can also comprise:
Image storage and display unit 123, for storing and showing that described scanning imagery unit 122 carries out opticsFunctional diagram and the optical section figure of the pathology sample tissue that scanning imagery processing obtains.
Preferably, described optical imagery module 12 can also comprise:
Graphics processing unit 124, for functional diagram and light that described image storage and display unit 123 is storedLearn slice map and carry out digital processing.
Wherein, the view data that image storage and display unit 123 obtains batch scanning imaging is according to useFamily parameter setting is stored in local computer, and is shown to operation user for intuitive judgment. User can be certainlyDefinition arranges the form, naming method, display mode of image storage etc.
The view data that graphics processing unit 124 can obtain batch scanning imaging is done digital processing,To improve contrast and the display effect of image. User can do image according to self-demand level and smooth, filtering,Go the operations such as background.
Described data analysis module 13, functional diagram and optical section that described optical imagery module 12 is obtainedFigure is analyzed with existing illness database data, generates diagnostic result report.
Concrete, described data analysis module 13 comprises:
Pathogeny analytic unit 131, for contrasting illness data and the described optical imagery mould of existing databaseThe functional diagram that piece 12 obtains and optical section figure, organize the approaching disease of most probable to determine with detection pathology sampleDisease, and then infer disease source and evolutionary process.
Wherein, during with existing database Data Comparison, operation includes but not limited to image characteristics extraction, image spyLevy coupling, characteristics of image search etc.
Lesion nature analytic unit 132, for determining and analyzing lesion nature. Can judge pathology sample groupThe happening part knitted, clinical symptoms, biological behaviour etc., and further analyze the range size of operation plan,The diffusion of disorders post surgery or radical cure situation etc. For example, for neoplastic lesion, include but not limited to understand tumourWhether optimum, if malignant tumour, its spread condition of growing, infiltration scope, the degree of depth, has or not transfer,Whether want tumor resection edge tissues etc.
Diagnostic analysis unit 133, for carrying out medical diagnosis on disease analysis, provides diagnostic comments. By in conjunction with frontState analysis result and algorithms library and provide tentative diagnosis result. Providing of diagnostic result includes but not limited to algorithms libraryOptimum results, empirical Pathology Doctors ' analysis result etc.
Report generation unit 134, for generating and sending diagnosis report. Can form and send CDReport, provide the final scheme of operation. User can self-defined report template, parameter setting, naming methodDeng. Report content comprises pathogeny analysis, lesion nature analysis, diagnostic result etc.
The present embodiment, by sample prepare module to pathology sample tissue be fixed, optical transparency, fluorescence dyeAfter look is processed, then by optical imagery module, sample is prepared to pathology sample tissue prepared by module and carry out opticsImage scanning, can obtain functional diagram and the optical section figure of pathology sample tissue. This saturating by introducing lightThe mode that bright technology combines with optical image technology, can realize the optical section function of similar actual slice,Further avoid the shared time of actual slice. And, owing to not having actual slice, pathology sampleTissue morphology and structure are preserved complete, optical imagery effect definition can with the routine paraffin wax pathologic finding of cutting into slicesEffect compare favourably, for doctor provides diagnostic data comparatively accurately. In addition, the art maximum with current applicationMiddle pathologic finding-quick frozen-section pathologic finding is compared, and a kind of early diagnosis provided by the invention is examinedDisconnected system, can realize the quick diagnosis of 60 minutes in theory the soonest.
Embodiment bis-
The realization flow of the method that the early diagnosis that Fig. 2 shows the embodiment of the present invention two to be provided is diagnosed,Details are as follows:
In step S201, in patient body, extract pathology sample tissue.
In the embodiment of the present invention, can in patient body, extract pathology sample tissue, for follow-up to pathology sampleTissue treatment provides must material. The operation of extracting includes but not limited to cut, clamps and get, pierce through, peel off etc.
In step S202, adopt the fixing described pathology sample tissue of chemical reagent.
In the embodiment of the present invention, fixing pathology sample tissue refers to the composition solidifying rapidly in pathology sample tissueMaterial, and structure while keeping its live body, after fixing pathology sample tissue, can be conducive to follow-up to pathologyThe optical transparency of sample tissue is processed and fluorescent staining processing. The chemical reagent of fixing pathology sample tissue comprise butBe not limited to formalin, formaldehyde, phosphate buffers etc., are controlled at 10 to the fixing used time of pathology sample tissueAbout minute.
In step S203, adopt optical transparency agent to carry out optical transparency place to fixing described pathology sample tissueReason.
In the embodiment of the present invention, optical transparency processing refers to that the refractive index changing in pathology sample tissue do not mate,Increase the penetration power of light in pathology sample tissue, pathology sample tissue is carried out after optical transparency processing, canFacilitate subsequent optical image-forming module to carry out the operation of profound optical section to pathology sample tissue. After this pathologyThe complex operations of sample tissue when carrying out similar making paraffin section again, has reduced sample making greatlyTime. Different pathology sample tissues can specific aim be adopted to the clarifier of different formulations, different proportionings,As the clarifier in number of patent application CN104568553A, according to the situation of pathology sample tissue, can be suitableThe ratio of change fructose, polyethylene glycol, urea, glycerine etc. The optical transparency of pathology sample tissue is processed and usedIn time, was controlled at about 10 minutes.
In step S204, carry out fluorescent staining place to carrying out the pathology sample tissue that optical transparency processing obtainsReason.
In the embodiment of the present invention, to the fluorescent staining processing of pathology sample tissue, can facilitate subsequent optical to studyAs module, pathology sample tissue is carried out the operation of optical scanner imaging. The selection of fluorescent dye can according to afterThe micro imaging system of selecting when continuous optical imagery and determining, staining method include but not limited to fluorescence immunization coloration,Fluorescent antibody staining etc. The fluorescent staining processing used time to pathology sample tissue was controlled at about 10 minutes.
In step S205, place and fixedly carry out the pathology sample tissue that fluorescent staining processing obtains.
In the embodiment of the present invention, pathology sample tissue can be anchored on to the loading of functional form micro imaging systemOn platform, to guarantee that, in the process of subsequent optical imaging, pathology sample is not organized and can be moved. Wherein, sampleThis firm mode includes but not limited to screw locking, spring leaf top pressure etc.
In step S206, the pathology that adopts functional form micro imaging system to obtain carrying out fluorescent staining processingSample tissue carries out optical scanner imaging, obtains functional diagram and the optical section figure of pathology sample tissue.
In the embodiment of the present invention, by functional form micro imaging system, the horizontal two dimension of pathology sample tissue is sweptRetouch the functional diagram that obtains pathology sample tissue; The indulging pathology sample tissue by functional form micro imaging systemObtain the optical section figure of pathology sample tissue to scanning.
Preferably, after obtaining the functional diagram and optical section figure of pathology sample tissue, all right: storageAnd functional diagram and the optical section figure of the pathology sample tissue that obtains of reading scan, and to described functional diagram and lightLearn slice map and carry out digital processing.
Concrete, the view data that batch scanning imaging can be obtained arranges storage according to customer parameterIn local computer, and be shown to operation user for intuitive judgment. User can customize and image is set depositsThe form, naming method, display mode of storage etc.
The view data that imaging obtains to batch scanning is done digital processing, can improve the contrast of imageWith display effect. User can do image according to self-demand level and smooth, and filtering, goes the operations such as background.
In step S207, the functional diagram that scanning imagery processing is obtained and optical section figure and existing illness numberBe analyzed according to database data, generate diagnostic result report.
In the embodiment of the present invention, illness data and the optical imagery module that can first contrast in existing database obtainThe functional diagram arriving and optical section figure, organize the approaching illness of most probable to determine with detecting pathology sample, entersAnd deduction disease source and evolutionary process.
Wherein, during with existing database Data Comparison, operation includes but not limited to image characteristics extraction, image spyLevy coupling, characteristics of image search etc.
Determine again and analyze lesion nature.
Concrete, can judge the happening part, clinical symptoms, biological behaviour of pathology sample tissue etc.,And further analyze the range size of operation plan, the diffusion of disorders post surgery or radical cure situation etc. For example forWhether optimum neoplastic lesion, include but not limited to understand tumour, if malignant tumour, its diffusion of growingSituation, infiltration scope, the degree of depth, has or not transfer, whether wants tumor resection edge tissues etc.
Then carry out medical diagnosis on disease analysis, provide diagnostic comments.
Concrete, by providing tentative diagnosis result in conjunction with aforementioned analysis result and algorithms library. Diagnostic resultProvide and include but not limited to algorithms library optimum results, empirical Pathology Doctors ' analysis result etc.
Finally generate and send diagnosis report.
Concrete, can form and send CD report, provide the final scheme of operation. User canSelf-defined report template, parameter setting, naming method etc. Report content comprises pathogeny analysis, lesion natureAnalysis, diagnostic result etc.
Should be understood that in embodiments of the present invention, the size of the sequence number of above-mentioned each process does not also mean that execution is suitableThe priority of order, the execution sequence of each process should be definite with its function and internal logic, and should be real to the present inventionExecute routine implementation process and form any restriction.
One of ordinary skill in the art will appreciate that all or part of step realizing in the various embodiments described above methodBe can carry out the hardware that instruction is relevant by program to complete, corresponding program can be stored in a computer canRead in storage medium, described storage medium, as ROM/RAM, disk or CD etc.
Those of ordinary skill in the art can recognize, in conjunction with respectively showing of embodiment description disclosed hereinUnit and the algorithm steps of example, can come with the combination of electronic hardware or computer software and electronic hardwareRealize. These functions are carried out with hardware or software mode actually, depend on the application-specific of technical schemeAnd design constraint. Professional and technical personnel can realize with distinct methods each specifically should being used forDescribed function, but this realization should not thought and exceeds scope of the present invention.
Those skilled in the art can be well understood to, for convenience and simplicity of description, and foregoing descriptionThe specific works process of system, device and unit, can be with reference to the corresponding process in preceding method embodiment,Do not repeat them here.
In the several embodiment that provide in the application, should be understood that disclosed system, device and sideMethod, can realize by another way. For example, device embodiment described above is only schematic, for example, the division of described unit, is only that a kind of logic function is divided, and can have another when actual realizationOuter dividing mode, for example multiple unit or assembly can in conjunction with or can be integrated into another system, orSome features can be ignored, or do not carry out. Another point, shown or discussed coupling each other or straightConnecing coupling or communicate to connect can be by some interfaces, INDIRECT COUPLING or the communication connection of device or unit,Can be electrically, machinery or other form.
The described unit as separating component explanation can or can not be also physically to separate, asThe parts that unit shows can be or can not be also physical locations, can be positioned at a place, orAlso can be distributed on multiple NEs. Can select according to the actual needs wherein some or all ofThe object of the present embodiment scheme is realized in unit.
In addition, the each functional unit in each embodiment of the present invention can be integrated in a processing unit,Also can be that the independent physics of unit exists, also can be integrated in a unit in two or more unitIn.
If described function realizes also as independently production marketing or use using the form of SFU software functional unitTime, can be stored in a computer read/write memory medium. Based on such understanding, skill of the present inventionThe part that art scheme contributes to prior art in essence in other words or the part of this technical scheme can be withThe form of software product embodies, and this computer software product is stored in a storage medium, if compriseDry instruction in order to make a computer equipment (can be personal computer, server, or the network equipmentDeng) carry out all or part of step of method described in the present invention each embodiment. And aforesaid storage medium bagDraw together: USB flash disk, portable hard drive, read-only storage (ROM, Read-OnlyMemory), arbitrary access are depositedReservoir (RAM, RandomAccessMemory), magnetic disc or CD etc. are various can storage program generationThe medium of code.
The above be only the specific embodiment of the present invention, but protection scope of the present invention is not limited toThis, any be familiar with those skilled in the art the present invention disclose technical scope in, can expect easilyChange or replace, within all should being encompassed in protection scope of the present invention. Therefore, protection scope of the present invention shouldThe described protection domain with claim is as the criterion.
Claims (10)
1. a system for early diagnosis diagnosis, is characterized in that, described system comprises: sample systemStandby module, optical imagery module and data analysis module;
Described sample is prepared module and is comprised:
Tissue collecting unit, for extracting pathology sample tissue in patient body;
Organize fixed cell, for the pathology sample that adopts the fixing described tissue collecting of chemical reagent unit to extractTissue;
Organize optical transparency unit, for adopting optical transparency agent to the described fixing pathology sample of fixed cell of organizingTissue carries out optical transparency processing;
Tissue staining unit, for carrying out to the described optical transparency unit of organizing the pathology sample that optical transparency processing obtainsThis tissue carries out fluorescent staining processing;
Described optical imagery module comprises:
Sample placement unit, for placing and fixing described tissue staining unit and carry out fluorescent staining processing and obtainPathology sample tissue;
Scanning imagery unit, for adopting functional form micro imaging system to carry out glimmering to described tissue staining unitLight dyeing processes the pathology sample tissue obtaining and carries out optical scanner imaging, obtains pathology sample tissueFunctional diagram and optical section figure;
Described data analysis module, the functional diagram that described scanning imagery unit is obtained and optical section figure are with existingThere is illness database data to be analyzed, generate diagnostic result report.
2. the system as claimed in claim 1, is characterized in that, the described optical transparency unit of organizing is to described groupKnitting the fixing pathology sample tissue of fixed cell carries out optical transparency processing and is specially: change in pathology sample tissueRefractive index do not mate, increase the penetration power of light in pathology sample tissue.
3. the system as claimed in claim 1, is characterized in that, described scanning imagery unit comprises:
The first imaging subelement, for by functional form micro imaging system to pathology sample tissue horizontal twoDimension scanning obtains the functional diagram of pathology sample tissue;
The second imaging subelement, for longitudinally the sweeping pathology sample tissue by functional form micro imaging systemRetouch the optical section figure that obtains pathology sample tissue.
4. system as claimed in claim 3, is characterized in that, described scanning imagery unit also comprises:
Image storage and display unit, for storing and showing described the first imaging subelement and the second imaging singlyFunctional diagram and the optical section figure of the pathology sample tissue that unit's scanning obtains;
Graphics processing unit, for the functional diagram to the storage of described image storage and display unit and optical section figureCarry out digital processing.
5. the system as claimed in claim 1, is characterized in that, described data analysis module comprises:
Pathogeny analytic unit, sweeps for the illness data and the described optical imagery module that contrast existing databaseRetouch the functional diagram and the optical section figure that obtain, infer disease source and evolutionary process;
Lesion nature analytic unit, for determining and analyzing lesion nature;
Diagnostic analysis unit, for carrying out medical diagnosis on disease analysis, provides diagnostic comments;
Report generation unit, for generating and sending diagnosis report.
6. a method for early diagnosis diagnosis, is characterized in that, described method comprises:
In patient body, extract pathology sample tissue;
Adopt the fixing described pathology sample tissue of chemical reagent;
Adopt optical transparency agent to carry out optical transparency processing to fixing described pathology sample tissue;
Carry out fluorescent staining processing to carrying out the pathology sample tissue that optical transparency processing obtains;
Place and fixedly carry out the pathology sample tissue that fluorescent staining processing obtains;
Adopt functional form micro imaging system to carry out light to the pathology sample tissue that carries out fluorescent staining processing and obtainLearn scanning imagery processing, obtain functional diagram and the optical section figure of pathology sample tissue;
It is right that the functional diagram that scanning imagery processing is obtained and optical section figure and existing illness database data carry outThan analyzing, generate diagnostic result report.
7. method as claimed in claim 6, is characterized in that, the agent of described employing optical transparency is to fixing instituteStating pathology sample tissue carries out optical transparency processing and is specially: the refractive index changing in pathology sample tissue is not mated,Increase the penetration power of light in pathology sample tissue.
8. method as claimed in claim 6, is characterized in that, described employing functional form micro imaging systemCarry out optical scanner imaging to carrying out the pathology sample tissue that fluorescent staining processing obtains, obtain pathology sampleThe functional diagram of this tissue and optical section figure, comprising:
By functional form micro imaging system, the horizontal two-dimensional scan of pathology sample tissue is obtained to pathology sample groupThe functional diagram of knitting;
By functional form micro imaging system, the longitudinal scanning of pathology sample tissue is obtained to pathology sample tissueOptical section figure.
9. method as claimed in claim 8, is characterized in that, at described employing functional form micro-imaging isSystem carries out optical scanner imaging to carrying out the pathology sample tissue that fluorescent staining processing obtains, and obtains pathologyAfter the functional diagram of sample tissue and optical section figure, also comprise:
Functional diagram and the optical section figure of the pathology sample tissue that storage reading scan obtain;
Described functional diagram and optical section figure are carried out to digital processing.
10. method as claimed in claim 6, is characterized in that, described scanning imagery processing is obtainedFunctional diagram and optical section figure and existing illness database data are analyzed, and generate diagnostic result report,Comprise:
Illness data and described optical imagery module in contrast existing database scan the functional diagram and the light that obtainLearn slice map, infer disease source and evolutionary process;
Determine and analyze lesion nature;
Carry out medical diagnosis on disease analysis, provide diagnostic comments;
Generate and send diagnosis report.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106370488A (en) * | 2016-08-23 | 2017-02-01 | 宁波江丰生物信息技术有限公司 | Cycle tumor cell slice-based diagnosis system and method |
| CN106548038A (en) * | 2016-12-09 | 2017-03-29 | 江西中科九峰智慧医疗科技有限公司 | A kind of Knowledge based engineering medical imaging acquisition analysis system |
| CN109979588A (en) * | 2019-01-22 | 2019-07-05 | 北京金智拓科技有限公司 | Image scanning pathological section system |
| CN110398503A (en) * | 2019-02-27 | 2019-11-01 | 广西壮族自治区农业科学院 | A kind of plant pest method of inspection based on geometric shape transmission measurement |
| CN110799843A (en) * | 2017-08-22 | 2020-02-14 | 莱卡生物系统墨尔本私人有限公司 | System and method for monitoring tissue samples to be processed by a tissue processor |
| CN111795879A (en) * | 2020-07-14 | 2020-10-20 | 青岛大学附属医院 | Application of pathological specimen manufactured by dehydration set in digital evaluation system |
| CN112529907A (en) * | 2020-10-19 | 2021-03-19 | 江苏珂玛麒生物科技有限公司 | SR staining-based rat lung fiber analysis system and working method thereof |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0994388A1 (en) * | 1998-10-14 | 2000-04-19 | Eastman Kodak Company | Medical diagnostic film for soft tissue imaging (II) |
| US6102928A (en) * | 1990-03-02 | 2000-08-15 | General Surgical Innovations, Inc. | Method of dissecting tissue layers |
| US6353753B1 (en) * | 1998-05-05 | 2002-03-05 | Stephen Thomas Flock | Optical imaging of deep anatomic structures |
| US7194118B1 (en) * | 2000-11-10 | 2007-03-20 | Lucid, Inc. | System for optically sectioning and mapping surgically excised tissue |
| CN101788709A (en) * | 2010-03-10 | 2010-07-28 | 广西大学 | Digital confocal microscope optical section collecting device |
| CN101487775B (en) * | 2009-01-20 | 2010-12-29 | 天津大学 | Use of borneol preparation in tissue optical clearing agent preparation |
| CN102284066A (en) * | 2011-05-09 | 2011-12-21 | 南开大学 | Novel application of whiteruss as optical transparency reinforcing agent |
| CN102301269A (en) * | 2008-12-05 | 2011-12-28 | 优尼森索股份公司 | Optical Sectioning Of A Sample And Detection Of Particles In A Sample |
| CN102749231A (en) * | 2011-10-14 | 2012-10-24 | 华中科技大学 | Optical clearing agent for bone tissue |
| CN104568553A (en) * | 2014-12-30 | 2015-04-29 | 深圳先进技术研究院 | Tissue optical clearing agent and application thereof |
-
2015
- 2015-12-22 CN CN201510967267.2A patent/CN105606573B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6102928A (en) * | 1990-03-02 | 2000-08-15 | General Surgical Innovations, Inc. | Method of dissecting tissue layers |
| US6353753B1 (en) * | 1998-05-05 | 2002-03-05 | Stephen Thomas Flock | Optical imaging of deep anatomic structures |
| EP0994388A1 (en) * | 1998-10-14 | 2000-04-19 | Eastman Kodak Company | Medical diagnostic film for soft tissue imaging (II) |
| US7194118B1 (en) * | 2000-11-10 | 2007-03-20 | Lucid, Inc. | System for optically sectioning and mapping surgically excised tissue |
| CN102301269A (en) * | 2008-12-05 | 2011-12-28 | 优尼森索股份公司 | Optical Sectioning Of A Sample And Detection Of Particles In A Sample |
| CN101487775B (en) * | 2009-01-20 | 2010-12-29 | 天津大学 | Use of borneol preparation in tissue optical clearing agent preparation |
| CN101788709A (en) * | 2010-03-10 | 2010-07-28 | 广西大学 | Digital confocal microscope optical section collecting device |
| CN102284066A (en) * | 2011-05-09 | 2011-12-21 | 南开大学 | Novel application of whiteruss as optical transparency reinforcing agent |
| CN102749231A (en) * | 2011-10-14 | 2012-10-24 | 华中科技大学 | Optical clearing agent for bone tissue |
| CN104568553A (en) * | 2014-12-30 | 2015-04-29 | 深圳先进技术研究院 | Tissue optical clearing agent and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 陈丹 等: "现代医学成像技术新进展", 《滨州医学院学报》 * |
| 陈煌林: "光透明剂对生物组织二次谐波成像的影响", 《中国优秀硕士学位论文全文数据库 基础科学辑》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106370488A (en) * | 2016-08-23 | 2017-02-01 | 宁波江丰生物信息技术有限公司 | Cycle tumor cell slice-based diagnosis system and method |
| CN106548038A (en) * | 2016-12-09 | 2017-03-29 | 江西中科九峰智慧医疗科技有限公司 | A kind of Knowledge based engineering medical imaging acquisition analysis system |
| CN110799843A (en) * | 2017-08-22 | 2020-02-14 | 莱卡生物系统墨尔本私人有限公司 | System and method for monitoring tissue samples to be processed by a tissue processor |
| CN110799843B (en) * | 2017-08-22 | 2023-08-22 | 莱卡生物系统墨尔本私人有限公司 | System and method for monitoring tissue samples to be processed by a tissue processor |
| CN109979588A (en) * | 2019-01-22 | 2019-07-05 | 北京金智拓科技有限公司 | Image scanning pathological section system |
| CN110398503A (en) * | 2019-02-27 | 2019-11-01 | 广西壮族自治区农业科学院 | A kind of plant pest method of inspection based on geometric shape transmission measurement |
| CN111795879A (en) * | 2020-07-14 | 2020-10-20 | 青岛大学附属医院 | Application of pathological specimen manufactured by dehydration set in digital evaluation system |
| CN112529907A (en) * | 2020-10-19 | 2021-03-19 | 江苏珂玛麒生物科技有限公司 | SR staining-based rat lung fiber analysis system and working method thereof |
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