CN105593679A

CN105593679A - Ph indicator device and formulation

Info

Publication number: CN105593679A
Application number: CN201480055581.7A
Authority: CN
Inventors: J.K.希克斯; V.J.哈蒙德
Original assignee: Smith and Nephew PLC
Current assignee: Smith and Nephew PLC
Priority date: 2013-10-08
Filing date: 2014-10-08
Publication date: 2016-05-18
Anticipated expiration: 2034-10-08
Also published as: BR112015015287A2; WO2015052225A1; CN108204972A; EP3055689B1; US9829471B2; GB201317746D0; RU2015139562A; JP6396481B2; AU2014333841B2; CN105593679B; JP2016535856A; US20180196021A1; ZA201503770B; US10288590B2; CA2895628A1; EP3055689A1; AU2014333841A1; US20150308994A1; RU2015139562A3

Abstract

Disclosed herein are devices and methods for determining the pH of fluid. Example devices include a device comprising a surface configured to contact the fluid and a pH indicator covalently bound thereto, wherein the pH indicator has a first colour prior to contact with the fluid and changes colour as a function of the pH of the fluid.

Description

PH indicator device and preparation

Background of invention

The pH's of test fluid flow sample need to be the requirement of many industries reliably, particularly wherein pH indicate potential quality,The industry of security or health problem. PH measures in for example medicine, biology, chemistry, agricultural, forestry, Food Science, environment section, oceanography, civil engineering, Chemical Engineering, nutrition, water treatment and water are important in purifying.

The pH of test water termly. The pH of routine monitor drinking water is to guarantee that it is safe for drinking, and routine test tripWater in swimming pool is to guarantee that it is safe for swimming therein. The pH of monitoring fish pond or river changes can indicative for environments dirtDye. In agricultural and gardening, know that the pH of soil not only can instruct the suitable crops of selection, can also distinguish and whether existLocal environment problem, as polluted. At food and brewing industry, keep suitable pH scope to send out in Food & Drink processIn raw many physical and chemical reactions, be vital. The pH of monitoring body fluid may be useful diagnostic method. For example,Verified, the pH of saliva can predict the neurological susceptibility to a series of diseases, comprises cancer, heart disease and osteoporosis.

PH test is carried out with pH meter conventionally, but these are difficult to carry out for many application, because they needCarry out periodic calibration with standard buffer solution. In addition, glass electrode is fragile, and must often keep moistening, conventionallyElectrode function obstacle to avoid the dehydration of pH sensing membrane and to cause thereupon in acid solution. Disposable pH test paper is available,But owing to changing the persistence of color with the pH of test sample, test paper can not be shown any rheological parameters' change with time of pH. In addition, oneThe feature of inferior property has increased cost meaning.

Exist for can be real-time, reversible and needs of the device of pH in test fluid stably.

Summary of the invention

The application discloses the apparatus and method that relate to the device with the indicator of the pH for monitoring fluid pH. Other advantage andImproving to those skilled in the art will be apparent in the time reading the application.

In one aspect, be provided for the device of the pH that measures fluid sample. This device preferably includes and is configured to contactThe pH indicator that the surface of this fluid and covalency are fixed thereon, wherein pH indicator with this fluid contact before have firstColor along with the pH of this fluid changes color along color spectrum (colourspectrum). In embodiments, pH indicator ringsAnswer the change of pH and change color, and at for example about 0.1 unit, about 0.2 unit, about 0.3 unit, about 0.4 listUnder the interval at position or about 0.5 pH of unit interval, this change color can detect. It is envisaged for, detection level is by basisTesting tool type used and changing. The electronic detectors of change color that for example, can detect light are as colour meter (colourMeter) can detect 0.1 unit pH change. By comparison, human eye only can visual detection and the pH of about 0.5 unitChange relevant change color. In embodiments, the pH indicator using in this device can detect about pH0 extremelyThe approximately pH of pH14, and indicate pH to change by the mode of the change color along color spectrum, shades of colour and specific pH in this spectrumRelevant. In embodiments, pH indicator can detect the pH of about pH5.0 to about pH10.0. In embodiments, pHIndicator can detect the pH of about pH5.5 to about pH9.5. More particularly, pH indicator can detect about pH6.5To the pH of about pH9.5. Suitable pH indicator comprises phenylazo compound, those as enumerated in table 1, and it can be available fromFraunhoferEMFT,Germany。

Table 1: phenylazo compound

Numbering	Chemical name
		GJM-514	2-[4 (2-hydroxyethyl sulfonyl)-phenyl] diazenyl]-4-methylphenol
GJM-546	1-hydroxyl-4-[4[(hydroxyethyl sulfonyl)-phenylazo]-naphthalene-2-sulfonic acid salt/ester 1 -->
		GJM-492	The fluoro-4-[4[(2-hydroxyl ethane of 2-sulfonyl)-phenylazo]-6-metoxyphenol
GJM-534	4-[4-(2-hydroxyethyl sulfonyl)-phenylazo]-2,6-syringol

In some embodiments, pH indicator is triarylmethane dye. In some embodiments, pH indicator isFluorescent dye.

In embodiments, the combination of pH indicator inclusion compound, described combination allows to detect than single by usingThe wider pH scope that compound can detect. For example, the combination that pH indicator comprises phenylazo compound. In embodimentIn, this combination comprises at least two kinds of phenylazo compounds that are selected from group cited in table 1. In embodiments, this combinationComprise at least three kinds of phenylazo compounds that are selected from group cited in table 1. In embodiments, this combination comprises at least oneThe phenylazo compound that kind is selected from group cited in table 1 is not the compound of phenylazo compound with at least one. ?In embodiment, derivative or the trim of the phenylazo compound of enumerating in imagination table 1.

In embodiments, this device is cellulosic material, for example cellulose liner. In embodiments, this device isNonwoven web or membrana perforata.

In some embodiments, this fluid is liquid. Limiting examples comprises water. In some embodiments, shouldFluid is gas, for example, for face shield. In some embodiments, this fluid is moisture. Limiting examples comprises and soilThe moisture that sample is relevant. In some embodiments, this fluid is body sample. Limiting examples comprises saliva, urine, bloodLiquid, sweat/sweat.

In another aspect, be provided for measuring the device of fluid sample pH. This device preferably includes: (a) fluid contactSurface, (b) relative nonfluid contact surface, the pH indicator that (c) comprises the pH indicator that covalency is fixed therein, this pH refers toShow the pH of agent instruction fluid, the wherein change of the pH of this fluid of color response of pH indicator and changing, and (d) at least one useIn the conduit towards pH indicator guiding fluid. This conduit contributes to towards pH indicator guiding fluid, and can not lead to fingerShow and in the way of agent, significantly change this pH. In certain embodiments, the material of this conduit does not contain acid or alkali official energy, that is to say,It is neutral, and can from fluid, not remove any acid or alkali entity, until it arrives pH indication mechanism. In some enforcementIn scheme, this device has outer surface, and pH indicator is positioned at this outer surface or is positioned near this outer surface. In other enforcement sideIn case, this device has the neighboring of extending between fluid contact surfaces and relative nonfluid contact surface, pH instructionDistrict is positioned at this neighboring or is positioned near this neighboring. In certain embodiments, conduit side direction guiding fluid is towards thisPH indicator. In embodiments, pH indicator is corresponding to the change of pH and change color, and this change color is for example aboutUnder the interval at 0.1 unit, about 0.2 unit, about 0.3 unit, about 0.4 unit or about 0.5 pH of unit interval, be to examineSurvey. Imagination, detection level will change according to testing tool type used. For example, electronic detectors are as colour meterThe pH that can detect 0.1 unit changes. By comparison, human eye only can visual detection change relevant to the pH of about 0.5 unitChange color. In embodiments, the pH indicator using in this device can detect the pH between pH0 to 14, and logicalCross along the mode of the change color of color spectrum and indicate pH to change, in this spectrum, shades of colour is relevant to specific pH. In embodiments,This pH indicator can detect the pH of about pH5 to about pH10. Especially, this pH indicator can detect about pH5.5To the pH of about pH9.5. More particularly, this pH indicator can detect the pH of about pH6.5 to about pH9.5. SuitablePH indicator comprises phenylazo compound, those of the group of enumerating in table 1 as being selected from. In embodiments, pH indicator bagContaining the combination of compound, described combination allows to detect the wider pH scope than detecting by use single compound. ExampleAs, the combination that pH indicator comprises phenylazo compound. In embodiments, this combination comprises at least two kinds and is selected from table 1The phenylazo compound of cited group. In embodiments, this combination comprises at least three kinds and is selected from group cited in table 1Phenylazo compound. In embodiments, this combination comprises at least one and is selected from the phenylazo of group cited in table 1Compound is not the compound of phenylazo compound with at least one. In embodiments, the phenyl of enumerating in imagination table 1 is evenThe derivative of nitrogen compound or trim.

In aspect another, be provided for the preparation of the pH that indicates fluid. Advantageously, pH indicator covalency is fixed on this systemIn agent and therefore, can in the time of contact, do not washed away by fluid. Said preparation preferably comprises the dyestuff that serves as pH indicator. This dyestuff canComprise phenylazo compound, wherein the change of the pH of the color response fluid of this phenylazo compound and changing. ImplementingIn scheme, the pH that this pH dyestuff responds 0.5 unit gap changes and change color. For example, this pH indicator is for each 0.5 unitThe pH change at interval has different colours. The pH indicator using in this device can detect pH5 to 10, be in particular pH5.5 to 9.5 and be more particularly the pH of pH6.5 to 9.5. Suitable pH indicator comprises phenylazo compound, as is selected from table 1In those of the group enumerated. In embodiments, this pH indicator comprises permission detection than examining by use single compoundThe combination of the compound of the wider pH scope measuring. For example, the combination that this pH indicator comprises phenylazo compound. In realityExecute in scheme, this combination comprises at least two kinds of phenylazo compounds that are selected from the group of enumerating in table 1. In embodiments, shouldCombination comprises at least three kinds of phenylazo compounds that are selected from the group of enumerating in table 1. In embodiments, this combination comprises at leastA kind of phenylazo compound that is selected from the group of enumerating in table 1 is not the compound of phenylazo compound with at least one. ?In embodiment, derivative or the trim of the phenylazo compound of enumerating in imagination table 1. In embodiments, by this systemAgent is applied to for detection of the device of pH during fabrication. In embodiments, said preparation is a kind of adhesive. In embodimentIn, this adhesive is a kind of low cohesive adhesive, for example silicone (silicon) adhesive. In other embodiments, contemplated, said preparation is a kind of gel, for example, the semi-rigid or rigid gel of suitable type, its not can with fluid contact to be testedTime disintegration. Said preparation can be used for according to first and/or the device of second aspect.

The method of pH for monitoring fluid is provided in another aspect. The method preferably includes following steps: (a)Provide and comprise the surperficial device that is configured to this fluid of contact, described surface has covalent bond pH indicator thereon,Wherein this pH indicator with fluid contact before there is the first color, and change color along with the pH of fluid, (b) make this dressPut and this fluid contact, (c) assess the color of this pH indicator. It is envisaged for, the method can be used for many application, at theseIn application, know that the pH of fluid sample is most important to determining quality control or security. The limiting examples of potential applicationComprise: food storing; Rotten (spoilage) indicator of packaging; Red wine; Wine brewing; Drinking water, swimming-pool water, river or fish pondAnalyze; Agricultural and gardening; Clothes, for example sweat analysis; The on-line monitoring of technique, gas, liquid; Skin nursing-medicine (skinSection) or cosmetics; Detect change/inconsistent container coating and surface; Monitoring medicine discharges or stability.

In aspect another, device comprises the fluid contact surfaces with covalent bond pH marking tools thereon, itsIn this pH marking tools with fluid contact before there is the first color, and change color along with the pH of fluid.

In another aspect, device comprises pH marking tools, wherein this pH marking tools with fluid contact before haveThe first color, and change color along with the pH of fluid, and for the conduit instrument towards this pH marking tools guiding fluid.

After the reading disclosure, those skilled in the art can change and revise these embodiments. Can be withAny combination and sub-portfolio (comprising multiple subordinate combinations and sub-portfolio) adopt described one or more further features hereinRealize above-mentioned feature and aspect. Above describe or illustrative various feature, comprise its any component, can be in other systemMiddle combination or integrated. In addition, some feature can be omitted or not implement.

Other field of the applicability of disclosed apparatus and method will become obvious by the detailed description hereinafter providing.Should be appreciated that this detailed description and instantiation, in instruction when particular, be only intended to illustration purpose but not be intended to limit these public affairsOpen the scope of any claim that maybe may pursue.

Accompanying drawing is described

By reference to the accompanying drawings, below considering while describing in detail by understanding more fully aforementioned and other object and advantage, wherein identicalReference numeral refers to identical parts in the whole text. It is illustrative but not with any side that the embodiment of these descriptions is interpreted asFormula restriction:

Figure 1A and 1B are the sectional views with the illustrative apparatus of pH indicator, and its color changes because the pH of fluid changes.

Fig. 2 A and 2B are the sectional views of illustrative apparatus, and wherein fluid guides to the pH that comprises pH indicator via conduitIndicator, the color of this indicator changes because the pH of fluid changes.

After Fig. 3 is the Post-Op(operation with GJM-514 dyeing) photo of sample, show that response is along pH unit gapThe dye colour of the solution of scale change pH changes.

Fig. 4 A-F is a colour examining measured value (colourpenmeasurements) of the Post-Op sample that shows in Fig. 3Diagram.

Fig. 5 is the photo with the Post-Op sample of the first dye combinations dyeing, has shown that response is along pH unit gap scaleChange the dye combinations change color of the solution of pH.

Fig. 6 A-D is the diagram of a colour examining measured value of the Post-Op sample that shows in Fig. 5.

Fig. 7 is the photo with the Post-Op sample of the second dye combinations dyeing, has shown that response is along pH unit gap scaleChange the dye combinations change color of the cushioning liquid of pH.

Fig. 8 A-E is the diagram of a colour examining measured value of the Post-Op sample that shows in Fig. 7.

Fig. 9 is the photo with the Post-Op sample of the 3rd dye combinations dyeing, has shown that response is along pH unit gap scaleChange the dye combinations change color of the cushioning liquid of pH.

Figure 10 A-F is the diagram of a colour examining measured value of the Post-Op sample that shows in Fig. 9.

Figure 11 is the photo with the Post-Op sample of the 4th dye combinations dyeing, has shown to respond to mark along pH unit gapDegree changes the dye combinations change color of the cushioning liquid of pH.

Figure 12 A-E is the diagram of a colour examining measured value of the Post-Op sample that shows in Figure 11.

Figure 13 A-F is the photograph that wherein alternately pumps into the responsive gauze of pH in the external wound model of horse serum of pH5 and pH8Sheet.

Figure 13 A is the photo of pH5 after about 2.5 hours.

Figure 13 B is the photo of pH5 after about 5.5 hours.

Figure 13 C is the photo of pH8 after about 8 hours.

Figure 13 D is the photo of pH5 after about 3.5 hours.

Figure 13 E is the photo of pH5 after about 5.5 hours, and the flow velocity of horse serum was located at 3.5 hours to improve.

Figure 13 F is the photo of pH5 after 7.5 hours, and the flow velocity of horse serum was located at 3.5 hours and 5.5 hours to improve.

Figure 14 A to F wherein alternately pumps into the responsive foam of pH in the external wound model of horse serum of pH5 and pH8The photo of (V.A.C.WhiteFoam, the trade mark of KCI).

Figure 14 A is the photo after about 2.5 hours under pH5.

Figure 14 B is the photo after about 5.5 hours under pH5.

Figure 14 C is the photo after about 15 hours under pH8.

Figure 14 D is the photo after about 3.5 hours under pH5.

Figure 14 E is the photo after about 5.5 hours under pH5, and the flow velocity of horse serum was located at 3.5 hours to improve.

Figure 14 F is the photo after about 7.5 hours under pH5, and the flow velocity of horse serum was located to carry at 3.5 hours and 5.5 hoursHigh.

Figure 15 A to E is the photo that wherein alternately pumps into pH sensitivity gauze in the external wound model of alkalescence and acid water.

Figure 15 A is the photo at the 1st day 8 a.m. demonstration alkaline pH.

Figure 15 B is at the 1st afternoon 12:57(5 hour) show the photo of alkaline pH.

Figure 15 C is at 08:03(24 hour in the 2nd day morning) show the photo of alkaline pH.

Figure 15 D is at the 2nd afternoon 12:41(5 hour) show the photo of acid pH.

Figure 15 E is at the 2nd day 15:06(7 hour) show the photo of acid pH.

Figure 15 F is at the 2nd day 16:47(9 hour) show the photo of acid pH.

Figure 16 A to F is the photo that wherein alternately pumps into pH sensitivity foam in the external wound model of alkalescence and acid water.

Figure 16 A is the photo at the 1st day 8 a.m. demonstration alkaline pH.

Figure 16 B is at the 1st afternoon 12:57(5 hour) show the photo of alkaline pH.

Figure 16 C is at 08:03(24 hour in the 2nd day morning) show the photo of alkaline pH.

Figure 16 D is at the 2nd day 09:06(1 hour) show the photo of acid pH.

Figure 16 E is at the 2nd day 15:06(7 hour) show the photo of acid pH.

Figure 16 F is at the 2nd day 16:47(9 hour) show the photo of acid pH.

Figure 17 A to H wherein alternately pumps in transparent organic glass (Perspex) wound model of alkaline and acid waterThe photo of the responsive foam of pH, alternately pumps into alkalescence and acid water.

Figure 17 A is the photo at the 1st day 8 a.m. demonstration alkaline pH.

Figure 17 B is at the 1st day 12:56(5 hour) show the photo of alkaline pH.

Figure 17 C is at the 1st day 16:20(8.5 hour) show the photo of alkaline pH.

Figure 17 D is at 8:02(24 hour in the 2nd day morning) show the photo of alkaline pH.

Figure 17 E is at 9:05(1 hour in the 2nd day morning) show the photo of acid pH.

Figure 17 F is at 10:50(3 hour in the 2nd day morning) show the photo of acid pH.

Figure 17 G is at the 2nd day 13:26(5.5 hour) show the photo of acid pH.

Figure 17 H is at the 2nd day 15:05(7 hour) show the photo of acid pH.

Detailed Description Of The Invention

In order to understand apparatus and method described herein, some illustrative embodiment and embodiment will be described now.

Figure 1A has described to have the device 100 of fluid contact surfaces 102 and relative nonfluid contact surface 104. Figure 1BDescribe the device 100 that it is contacted with fluid 106. Device 100 can be by any material that is suitable for the not disintegration with fluid contactMaterial is made.

This device also comprises pH indicator 108, is applied on the one or both sides on surface 102 and/or 104. PH refers toShow that agent covalency is fixed on surface 102 and/or 104 or adjacent to surface 102 and/or 104, so that it can not washed away by fluid.

In embodiments, this pH indicator chemically binds to surface 102 and/or 104. For example, this pH indicator is directBe covalently bond to surface 102 and/or 104. In alternate embodiment, surface 102 and/or 104 is arranged in adhesive, andAnd this pH indicator is covalently bond to the reactive part in this adhesive. For example,, for constructing the conventional propylene of wound dressingAcids adhesive, as K5(Smith&Nephew, Inc), the residue that contains methacrylic acid 2-hydroxyl-ethyl ester, it providesSide joint is to reactive functional hydroxyl (OH) group of polymer backbone, and this pH indicator can be covalently bond to this reactive functionalOn hydroxyl. Other suitable adhesive comprises the acrylic adhesive with OH or COOH side group.

Only pH indicator being applied in a surperficial embodiment of non-porous device, can provide and indicate subsequentlyPH indicator is applied to the instruction of which side. This instruction makes user can be the process of placing on fluid or in fluidIn be suitably orientated this device, there is the surperficial correct orientation of pH indicator and make it and fluid contact to guarantee.

This pH indicator can be across substantially whole surperficial 102 and/or 104 applying to allow to determine at the curved liquid of fluid sampleAny variation of the pH that face (meniscus) is located. Or this pH indicator can be applied to the discrete regions on surface 102 and/or 104Territory. This pH indicator with fluid contact before show the first color, and along with the pH of this fluid changes color. This pH indicatorThe first color can be colourless.

This pH indicator can respond pH and reversibly change color. In embodiments, this pH indicator is phenylazoCompound. In certain embodiments, this phenylazo compound is selected from the group of enumerating in table 1. In some embodiments, shouldPhenylazo compound is not 2-[4 (2-hydroxyethyl sulfonyl)-phenyl] diazenyl]-4-methylphenol. In some enforcementIn scheme, this phenylazo compound is not hydroxyl-4-[4[(hydroxyethyl sulfonyl)-phenylazo]-naphthalene-2-sulfonic acid salt/Ester. In some embodiments, this phenylazo compound is not the fluoro-4-[4[(2-hydroxyl ethane of 2-sulfonyl)-phenyl is occasionallyNitrogen]-6-metoxyphenol. In some embodiments, this phenylazo compound is not 4-[4-(2-hydroxyethyl sulphonylBase)-phenylazo]-2,6-syringol. In certain embodiments, this phenylazo compound is 2-[4 (2-hydroxylEthylsulfonyl)-phenyl] diazenyl]-4-methylphenol. In some embodiments, this pH indicator comprises multiple phenylAzo-compound. In some embodiments, this pH indicator comprises the combination of phenylazo compound, for example, be selected from table 1The combination of the phenylazo compound of the group of enumerating. In some embodiments, this pH indicator comprises two kinds of phenylazosThe combination of compound. In some embodiments, this pH indicator comprises the combination of three kinds of phenylazo compounds. In some enforcementIn scheme, 2-[4 (2-hydroxyethyl sulfonyl)-phenyl] diazenyl]-4-methylphenol and at least one be selected from row in table 1Other phenylazo compound combination of the group of lifting. The ratio of phenylazo compound can be 1:1, but it is contemplated that otherRatio, such as but not limited to 0.5:1.5 or 1.5:0.5 or 1:2 or 2:1 or 1:0.1. In alternate embodiment, this pHIndicator comprises at least one phenylazo compound, for example, be selected from the phenylazo compound of the group of enumerating in table 1, and alsoOther compound of at least one of non-phenylazo compound. In certain embodiments, this pH indicator is not phenylazoCompound.

Fig. 2 A&B has shown the device that wherein can monitor the transient change of pH at this device in original position. Fig. 2 AShown the sectional view of the device 200 that comprises absorber element 204, the lower surface of described absorber element 204 is fluid contact tablesFace 206. This device also comprises pH indicator 208, and it is positioned at relative nonfluid contact surface 210 places or adjacent to described surface210. This pH indicator comprises pH indicator (for example, pH indicator as disclosed herein), and it can respond pH variation canChange color contraryly. Shown in this, in embodiment, pH indicator 208 is arranged on absorbed layer 204 tops, makes this pH instructionAgent can be through time monitoring and remove this device without adhering to from it in any substrate of this device.

Hyaline layer 212 covers at least a portion pH indicator, and it protects the integrality of this pH indicator, but still allows to makeUser through time monitor the color of pH indicator. This device comprises that at least one is configured to fluid is guided to this pH indicator206 conduit, guarantees that the pH of fluid can significantly not change in the time of its assembly through this device. Can use one or more leadingPipe. As shown in Fig. 2 A&B, use two conduits, although also can comprise one or more other conduits. Two conduit 214 Hes216 is vertical orientated and extend across this device. This conduit preferably seals, so as not with absorbed layer replacement fluids, but and pHIndicator 208 is communicated with and fluid is guided to the pH indicator 208 that is positioned at this device top. This conduit can be thin capillaryForm, it is towards pH indicator 208 transmitting fluids. This conduit can or can be made up of wick material in conjunction with wick material, instituteState wick material for example for woven, non-woven, knitting, the tow be made up of suitable material or fiber are to promote this fluid towards pHThe wicking of indicator 208. In alternate embodiment, at lateral margin 218 or 220 places of this device or pH instruction is provided in its vicinityDistrict, and in this device, provide at least one conduit to guide this fluid to pH indicator with side direction. In some embodiments, existForm in the layer of this device of this outside of deivce face this pH indicator is provided, and do not use transparent cover layer. Real at someExecute in scheme, this conduit can be taked rectangular form, or when there is elongated diamond shape in the time that fluid contact surfaces is observed.Or conduit can be made up of cross or quadrangle form.

Also considered to fix the method for phenylazo dyestuff on the device shown in Fig. 1 and 2. An example comprises followingStep:

In first step, 25 milligrams of phenylazo pH indicating dyes, the phenylazo pH that is for example selected from the group of enumerating in table 1 refers toShow dyestuff, with 140 microlitre strong sulfuric acid response 30 minutes to form dye solution.

In second step, in the dye solution forming in first step, add 200 ml distilled waters.

In third step, in the solution forming in second step, add the NaOH of 406 microlitre 32%w/v moltenLiquid.

In the 4th step, to the sodium carbonate liquor that adds 25.45 milliliters of 2.36M in the solution forming in third step.

In the 5th step, in the solution forming in the 4th step, add the NaOH of 1.35 milliliters of 32%w/v moltenLiquid, and its volume is supplemented to 250 milliliters with distilled water.

In the 6th step, will be placed in this solution and make it react about 1-in conjunction with the material of pH indicating dye thereon2 hours. The example of suitable material includes but not limited to: the TENCEL fiber of Durafiber product, Allevyn product poly-The cellulosic mat of ammonia ester foam, OpsitePost-op product or scribble the polyurethane film of K5 adhesive, all can be available from Smith&Nephew, Inc. This material is subsequently with distilled water washing, until do not have dyestuff to be released. Subsequently that this material is dry.

Embodiment

Prepare the liner sample from OpsitePost-Op dressing (Smith&Nephew, Inc) in different sample kindsProduct, the combination covalent bond of each sample and a kind of phenylazo dyestuff or phenylazo dyestuff, described phenylazo dyestuff choosingFrom GJM-514, GJM-492, GJM-546 and GJM-534. The structure of these dyestuffs is presented in table 1. Have been found that these dyeMaterial has the color shifting properties changing according to the change of pH. Use as above made this Post-Op about the method as described in Fig. 1-3Sample and independent GJM-514 or with the combination covalent bond of GJM-514 and one of GJM-492, GJM-546 and GJM-534.Post-Op material is exposed to the cushioning liquid of the pH with 5,5.5,6,6.5,7,7.5,8,8.5,9 and 9.5. Absorb each sampleThe photo of product is to prove the visible change of color. Colour examining pen (for example DrLangeColourPen)---a kind of pen type colourityMeter---for detection of the immesurable small change color of human eye. A colour examining measured value includes but not limited to three kinds of different readingNumber: L*, a* and b* value.

Brightness/the luminosity of L* representative color

OL*=0 is black

OL*=100 is diffusivity white

A* is the color position between red/magenta and green

The positive a* value representation of o magenta

O bears a* value representation green

B* is the color position between yellow and blueness

The positive b* value representation of o yellow

O bears b* value representation blueness.

Embodiment 1: with the Post-OpPad of GJM-514 dyeing

Will with the covalently bound liner sample from OpsitePost-Op dressing (Smith&Nephew) of dyestuff GJM-514Product are exposed to the cushioning liquid under pH5-pH9.5. Photo plate in Fig. 3 has been shown the face of GJM-514 within the scope of this pHLook changes, by yellow (under pH5) extremely pink (under pH9.5).

Table 2 shown the color of GJM-514 dyestuff within the scope of the pH of pH5-pH9.5 a colour examining measured value (L*,A* and b*). Optimum dye as pH indicator is for example, survey at particular color parameter (L*, a* or b*) within the scope of wide pHValue aspect shows the dyestuff of linear change. Outside this range of linearity, this dyestuff can not respond the variation of pH and change faceLook, or the variation of color is too little to such an extent as to cannot detect.

pH	L*	a*	b*
				5	63.3	-1.9	41.5
5.5	69.2	0.3	36.2
				6	65.7	1.4	35.1
6.5	59.3	1.2	35.5
				7	56.9	2	33.6
7.5	55.4	4.8	30.6
				8	46.8	10.4	21.4
8.5	43.3	15.6	15.4
				9	40.2	21.3	8.7
9.5	37.5	24.8	4.9

Table 2.

Fig. 4 A and 4B have shown the L* measured value of the GJM-514 dyestuff of obtaining with colour examining pen presenting with graphics mode. FigureThe L* result of 4A shows, from pH5.5 to pH9.5, L* value reduces, because the luminosity of dyestuff reduces with respect to the pH improving.These results are also plotted in Fig. 4 B, and have shown the range of linearity between pH7.5 and 9.5. This Trendline has-8.18Slope and 0.9918 R²Value.

Fig. 4 C and 4D have shown the a* measured value of the GJM-514 dyestuff of obtaining with colour examining pen presenting with graphics mode. Figure4C has shown the a* measured value that the different pH values place between pH5-pH9.5 obtains. Fig. 4 D has shown between pH7.5 to 9The a* measured value at different pH values place in the linear segment of Trendline. This Trendline has 10.94 slope and 0.9997 R²Value.

Fig. 4 E and 4F have shown the figure description of the b* measured value that GJM-514 dyestuff is obtained. Fig. 4 E has shown at pH5-The b* measured value that different pH values place between pH9.5 obtains. Fig. 4 E has shown in the linear segment of Trendline in different pH valuesUnder b* measured value. Can be found out by Fig. 4 E, this value between pH5.5 to pH7, be suitable one make peace stable, pH7 itThey start to reduce afterwards. Fig. 4 F shows, this result has provided linear downward trend between pH7.5 to pH9, slope is-14.34, and R²Value is 0.991.

Consider the photo of a colour examining result and sample, the working range the most accurately of GJM514 be pH7.5 to pH9 itBetween. The linear trend of this b* measured value has the slope (14.34) steeper than a* measured value (10.94), therefore ought make to use upWhile learning reader instead of human eye, will preferably use b* so that the more accurately instruction of dressing pH to be provided.

Embodiment 2: with GJM-514:GJM-492(1:1) Post-OpPad of dyeing

By being 1:1 with ratio, dyestuff GJM-514:GJM-492 is covalently bound from OpsitePost-Op dressing (SmithGasket sample &Nephew) is exposed to the cushioning liquid under pH5-pH9.5. Photo plate in Fig. 5 has been shown at this pHChange color in scope, by yellow (under pH5) extremely orange (under pH9.5).

Table 3 has shown the colour examining of the color of GJM-514:GJM-492 dye combinations within the scope of the pH of pH5-pH9.5Measured value (L*, a* and b*).

pH	L*	a*	b*
				5	53.8	11.5	43.3 8 -->
5.5	50.7	17.4	37.9
				6	45.3	23.9	37.5
6.5	40.4	29.9	35.4
				7	39.7	30.9	33.8
7.5	39.9	30.4	29.9
				8	34.5	31.5	29.2
8.5	37.4	28	29.3
				9	33.8	30.7	25
9.5	33.1	31.3	23.2

Table 3.

Fig. 6 A has shown the L* measured value of obtaining with colour examining pen presenting with graphics mode. The L* result table presenting in Fig. 6 ABright, in the scope from pH5.5 to pH9.5, L* value reduces, but does not follow linear downward trend. This L* value therefore not byBe considered as the reliable instruction of the change color of this dye combinations within the scope of tested pH.

Fig. 6 B and 6C have shown the a* measured value of obtaining with colour examining pen presenting with graphics mode. Fig. 6 B has shown at pHThe a* measured value that different pH values place between 5-pH9.5 obtains. Fig. 6 C has shown in the linear segment of Trendline at different pHThe a* measured value at value place. Between pH5 to 6.5, can determine linear trend (slope=12.34, R upwards²=0.9997), show to have detectable change color along red/pinkish red extremely green scale within the scope of this pH.

Fig. 6 D has shown the figure description of the b* measured value of obtaining with colour examining pen. Can find out, not have significant b* valueChange, but have downward trend.

The photo of considering a colour examining result and sample, the working range of this dye combinations appears as pH5 to pH6.5Between. A* has provided the available Trendline in this region, and it can be used for assessing pH by material color.

Embodiment 3: with GJM-514:GJM-546(1:1) Post-OpPad of dyeing

By being 1:1 with ratio, dyestuff GJM-514:546 is covalently bound from OpsitePost-Op dressing (Smith&Nephew) gasket sample is exposed to the cushioning liquid under pH5-pH9.5. Photo plate in Fig. 7 has been shown at this pH modelChange color in enclosing, by orange (under pH5) extremely pink (under pH9.5).

Table 4 has shown the colour examining of the color of GJM-514:GJM-546 dye combinations within the scope of the pH of pH5-pH9.5Measured value (L*, a* and b*).

pH	L*	a*	b*
				5	45.7	22.7	44.1
5.5	43.4	22.8	40.1
				6	43.9	24.8	34.6
6.5	36.5	27	25
				7	33.4	25.7	16
7.5	28.3	27.8	7.1
				8	26.9	26.6	1.3
8.5	25.6	29.3	-0.7
				9	24.5	28.8	-2.3
9.5	23.9	29.5	-3.8

Table 4.

Fig. 8 A and 8B have shown the figure description of the L* measured value of obtaining with colour examining pen. Fig. 8 A shows all data points, andFig. 8 B has repainted data point in the range of linearity between pH5 to pH8. This Trendline has-6.3702 slope, R²Value is 0.9982.

Fig. 8 C has shown that the a* obtaining with colour examining pen presenting with graphics mode within the scope of pH5-pH9.5 measuresValue. This result changes excessive to a* measured value to such an extent as to cannot consider to ring for measuring reliably GJM514:546 dye combinationsThe change color of answering pH to change.

Fig. 8 D and 8E have shown the figure description of the b* measured value of obtaining with colour examining pen. Fig. 8 E has shown at pH5-pHThe b* measured value of obtaining under different pH values between 9.5, and can find out, this result is followed to decline from pH5 to pH8 andGesture, but seem to occur platform after pH8. Fig. 8 E has shown the b* under the different pH in the linear segment of TrendlineMeasured value, this Trendline has-18.3 slope and 0.9997 R². Because b* result has provided more precipitous slope, thereforeIt is believed that monitoring b* value will be provided the more pin-point reading of pH by dressing color. The working range of this dye combinations appears as pH6 extremelypH7.5。

Embodiment 4: with GJM514:534(1:1) Post-OpPad of dyeing

By covalently bound from OpsitePost-Op dressing (Smith& with the dyestuff GJM514:534 of 1:1 ratioNephew) gasket sample is exposed to the cushioning liquid under pH5-pH9.5. Photo plate in Fig. 9 has been shown at this pH modelChange color in enclosing, by yellow (under pH5) extremely red (under pH9.5).

Table 5 has shown the colour examining of the color of GJM-514:GJM-534 dye combinations within the scope of the pH of pH5-pH9.5Measured value (L*, a* and b*).

pH	L*	a*	b*
				5	53.4	6.1	50.3
5.5	52.3	7.5	45.4
				6	53.8	7.6	46.1
6.5	49.7	9.8	35.4
				7	43.1	16.2	29.9
7.5	37.4	16.2	18.9
				8	33.4	20.4	11.9
8.5	31.9	22.8	5.3
				9	27.7	27.6	3.6
9.5	28.9	29.1	-0.5

Table 5.

Figure 10 A and 10B have shown the figure description of the L* measured value of obtaining with colour examining pen. Figure 10 A shows all data points,And Figure 10 B is only presented at those data points in the range of linearity. Observe the general downward trend by pH6 to pH9. This trendLine has-8.8286 slope and 0.9742 R²Value.

Figure 10 C and 10D have shown the a* measured value of obtaining with colour examining pen presenting with graphics mode. Figure 10 C has shownThe a* measured value that different pH values place between pH5-pH9.5 obtains. Figure 10 D has shown in the linear segment of Trendline notWith the a* measured value at pH value place. Result has shown the ascendant trend between pH6 to pH9, and this Trendline has 6.6335Slope and 0.9924 R²Value.

Figure 10 E and 10F have shown the figure description of the b* measured value of obtaining with colour examining pen. Figure 10 E has shown at pH5-The b* measured value of obtaining under different pH values between pH9.5, and can find out, this result is followed downward trend, until pH9. The Trendline showing in Figure 10 F has-16.314 slope and 0.9925 R between pH6 and pH9²Value. By this colour examiningMeasured value is known, and the working range of this dye combinations is between pH6 to pH9, and b* value can be used for by material color essenceReally measure pH.

Embodiment 5: with GJM514:534(1:0.509) Post-OpPad of dyeing

By being 1:0.509 with ratio, dyestuff GJM514:534 is covalently bound from OpsitePost-Op dressing (SmithGasket sample &Nephew) is exposed to the cushioning liquid under pH5-pH9.5. Photo plate in Figure 11 has been shown at this pHChange color in scope, by yellow (under pH5) extremely red (under pH9.5).

Table 6 has shown the colour examining of the color of GJM-514:GJM-534 dye combinations within the scope of the pH of pH5-pH9.5Measured value (L*, a* and b*).

pH	L*	a*	b*
				5	55.4	4.9	43.1
5.5	57.6	2.9	42.6
				6	56.8	3.4	42.7
6.5	51.2	5	40
				7	49	8.8	34.7
7.5	39.8	11.4	23.5
				8	39	17.6	15
8.5	36.5	22.4	10.1
				9	34.2	24.3	5.8
9.5	32.3	25.3	0.3

Table 6.

Figure 12 A has shown the figure description of the L* measured value of obtaining with colour examining pen. Observe by one of pH6 to pH9.5As downward trend.

Figure 12 B and 12C have shown the a* measured value of obtaining with colour examining pen presenting with graphics mode. Figure 12 B has shownThe a* measured value that different pH values place between pH5-pH9.5 obtains. Figure 12 C has shown in the linear segment of Trendline notWith the a* measured value at pH value place. Result has shown the linear ascendant trend between pH6.5 to pH8.5, and this Trendline has8.72 slope and 0.9987 R²Value.

Figure 12 D and 12E have shown the figure description of the b* measured value of obtaining with colour examining pen. Figure 12 D has shown at pH5-The b* measured value of obtaining under different pH values between pH9.5, and can find out, this result is abided by between pH6 and pH8.5Follow downward trend. The Trendline showing in Figure 12 E has 15.9 slope and 0.9833 R²Value. Consider a colour examining result andThe photo of sample, the working range of this dye combinations is between pH6 and pH8.5, and b* value can be used for by material color essenceReally measure pH.

Embodiment 6 and 7

About the conventional method of above-mentioned preparation covalent bond dyestuff, different materials also can be used in conjunction with this dyestuff.

Gauze (Kerlix, the trade mark of Covidiene) and polyvinyl alcohol foam (V.A.C.WhiteFoam, the business of KCIMark) sample as the disclosure in the whole text described in like that and ratio be 1:3.92 dyestuff GJM-546 and 492 covalent bond.

These materials below can be used as the pH sensing filler of negative pressure wound therapy (NPWT). By using with dragWith experiment, they are assessed.

Material

Material
	Pork (long saddle or boston butt, about 2 kilograms, skin is intact, about 20 × 20 centimetres of surface area)
The responsive VAC foam of pH
	The responsive gauze of pH
Renasys drop cloth
	Horse serum
Citric acid
	Sodium acid carbonate

Equipment

Equipment
	Renasys EZ plus pump
Peristaltic pump
	Renasys EZ tank
Epidural needle
	Preservative film
Pipeline
	Glass plate
Scalpel
	PH meter

Method

Use these solution that horse serum is adjusted to pH5 and pH8 for use in meat model.

1. place a slice preservative film at glass plate/tray bottom, and on this preservative film, place a slice and there is complete skin upwardsThe pork of skin.

2. pork pies are rolled in preservative film, increase if necessary more preservative films, so that meat is completely sealed.

3. by removing skin/fat/muscle, manufacture 2 wounds with scalpel, diameter is respectively done for oneself about 50 millimeters, at tissueIn the degree of depth be 25 millimeters (at least separately 2 centimetres), there is the bottom of relatively flat and minimum tissue valve prosthesis.

4. insert epidural catheter pin through wound side, make tip appear at the external margin place of meat. Use this pin to connectLogical peripump tubing, is located at wound bottom. (for other wound repeating said steps).

5. the Flexi-fix of use small pieces and/or caulk compound (" white-tac ") are fixing leaves preservative film with fluid-encapsulated pipeOpening.

6. the following combination of test:

A. the VAC foam of dyeing

B. the gauze of dyeing

7. add foam to be bridged to intact healthy skin and two bridgings are connected together to operated by single-port.On wound, filler and bridge joint foam, seal with drop cloth.

8. in drop cloth, manufacture aperture, it is positioned on foam bridge and uses Flexi-fix band connectivity port.

9. this port be connected to RENASYSNPWT pump (being set in-120 millimetress of mercury) and open.

10. open peristaltic pump (be set to and carry 40 mul/min) to carry the horse serum of pH8 to wound bed.

11. monitoring dressing are until fluid starts to appear at (attention time span) in tank.

12. change into fluid the horse serum of pH5 and keep flow step 11) in definite time quantum. Take subsequently dressingPhoto.

13. change into fluid the horse serum of pH8 and keep flow step 11) in definite time quantum. Take subsequently dressingPhoto.

14. become fluid to the horse serum of pH5 again and keep flow step 11) in definite time quantum. Take subsequently dressingPhoto.

15. in the time that experiment finishes, and disconnects pipeline, and seals the meat in preservative film to dispose. With soap/water clean all withThe surface that meat is contacted.

Determine that dyeing VAC foam and gauze detect the ability that in wound fluid, pH changes

After the first meat model experiment, wash the responsive gauze of pH and VAC foam, and subsequently for additional wound model, use pHThe water regulating. In addition, the responsive dyed yarn cloth of the pH of extra section is placed in transparent organic glass wound model and pumping fluidPass through.

All wound model are monitored by picked-up photo, and those that carry out in meat are only monitored from end face,But transparent organic glass model can be from the monitoring of all sides.

Result and discussion

This foam is orange in the time being packed into wound, but gauze has more redness. It is believed that gauze be redness be byIn there is PHMB on gauze, this is alkalescence.

Meat model 1

In fluid starts to appear at tank and before this material starts variable color, the horse serum of pH5 is pumped in Wound fillingAbout 2.5 hours, start thus this experiment. After about 5.5 hours, pH5 horse serum solution is become to pH8 horse serum, andAnd it is moved whole night. In the morning, solution is become again to pH5 horse serum subsequently and pump into several hours (due to time restriction,Flow velocity was increased to 80 mul/min after 3.5 hours).

The image of the responsive dyeing of pH gauze rheological parameters' change with time is found in Figure 13 A to F; Show that gauze is being exposed to pH5 horse serumAfter 5.5 hours, start to become orange, and reverted to redness being exposed to after one night of pH8 serum. Be exposed to pH subsequentlyAfter 5 a few hours, gauze starts again to transfer to orange, now finishes experiment. In the time removing gauze, can find out, gauze bottom is mainFor orange, can find out, color and pH are therefore being changed in the direction of drop cloth by wound bed in whole gauze, thisCan explain by the following fact: wound tends to be full of, as being full of bathtub, therefore when pump liquid is slow by Wound fillingWhen transmission, this pH spended time is to become another pH from a pH.

The VAC foam of the responsive dyeing of pH through time the image that changes be found in Figure 14 A to F. They have shown that this foam is cruellyWhile being exposed to pH5 horse serum, become yellow (5.5 hours images), and when being exposed to pH8 whole night time, this foam becomes redness.As with gauze, foam starts to become yellow/orange be again exposed to pH5 serum a few hours before experiment finishes after, this HuangLook/orange can the most clearly be seen near bridge joint foam.

Meat model 2

For the second meat model, first use alkaline aqueous solution and continued to pump into model whole night. Morning, subsequentlyThis solution is replaced by acidic aqueous solution and continues pumping a few hours.

The image of the responsive gauze of pH is found in Figure 15 A to F, and shows that this gauze becomes redness in alkaline solution, andIn fluid switches to acidic aqueous solution 5 hours, this gauze starts to become orange. It is believed that this change color opens in wound bottomBegin, and extend upwardly to this surface in the time that the pH in wound changes, it is described above, is similar to the side of wherein filling up bathtubFormula. Be apparent that, change color from the teeth outwards starts near the region being located immediately at below this port, and this can be interpreted asThis is the terminal (leaving a little) of fluid, and therefore this pH will be stable near this region first from the teeth outwards.

Adopt the VAC foam of dyeing also can see identical trend, as shown in Figure 16 A to F. When there being alkaline fluidsTime, this foam becomes redness, and in the time that fluid becomes acidity, this foam starts to become yellow. Be similar to gauze, at fluid from woundFirst the port removing can notice the change color of seeing from the teeth outwards around.

Transparent organic glass wound model

Also in transparent organic glass wound model, use the gauze of the responsive dyeing of pH to carry out this experiment to can see wholeThe change color of wound. In this case, this fluid is not pumped into by bottom, but is pumped into by the left-hand side of wound, asThat on the image in Figure 17 A to H, sees is such. The half of fluid intake on phase the same side of this port and on wound wallPlace. The area that it is believed that this wound is less than those that produce in meat, therefore in the time that the pump speed in two experiments is identical, and faceLook change occurs sooner. Can find out, in the time that alkaline fluids pumps into wound, gauze becomes redness and (located at T=0 hour, in woundIn mouthful, had some alkaline fluids, therefore a part for gauze has been red). By all figure in Figure 17 A to HCan find out, color change from left to right for picture---end face (top graph picture) of wound and bottom (each right bottom diagram picture)---Traversing through wound, and wound bottom is slightly prior to the upper surface of wound. This change color pattern as expected becauseFluid is filled by bottom, and therefore pH changes in bottom prior to top. It is so true to nature that this organic glass model is not so good as meat model,Because fluid and the content from meat mean due to possible buffering effect, pH may need the time of more growing to becomeChange.

Conclusion and suggestion

VAC foam and the gauze of the responsive dyeing of pH, variable color in the time that they are exposed to different pH solution. Be used to indicate the face of different pHLook is apparent, and this color can reverse by add other pH solution to wound.

It being understood that description is above only illustrative, be not limited to given details. Although in the disclosureSome embodiments are provided, should be appreciated that disclosed apparatus and method and their assembly can with many itsIts particular form is implemented, and does not leave the scope of the present disclosure.

Those skilled in the art will expect changing and amendment after the reading disclosure. Disclosed feature can be with hereinDescribed in one or more further features any combination and sub-portfolio (comprising combination and the sub-portfolio of multiple subordinates) enforcement. OnThe various features that literary composition is described or shown, comprise its any part, in other systems combination or integrated. In addition, certainA little features can be omitted or not implement.

The example that changes, replaces and change can be determined by those skilled in the art, and can not leave public affairs hereinIn the scope of the information of opening, carry out. All bibliography mentioned in this article are quoted with it and are incorporated in full herein and form this through thisA part for application.

Claims

1. for measuring the device of pH of fluid, described device comprises that the fluid with covalency pH indicator fixed thereon connectsTouch surface, wherein said pH indicator with described fluid contact before there is the first color, and along with the pH of described fluidAnd change color.

2. device according to claim 1, the change color in wherein said pH indicator becomes at the pH of 0.1 unit gapUnder changing, can record.

3. device according to claim 2, the change color in wherein said pH indicator is at extremely approximately pH of about pH5Between 10, can record.

4. device according to claim 3, the change color in wherein said pH indicator at about pH5.5 extremely approximatelyBetween pH9.5, can record.

5. according to the device described in claim 3 or 4, the change color in wherein said pH indicator is extremely large at about pH6.5Between about pH9.5, can record.

6. according to the device described in claim 1 to 5 any one, wherein said pH indicator comprises phenylazo compound.

7. device according to claim 6, wherein said phenylazo compound is selected from the group of enumerating in table 1.

8. according to the device described in claim 6 or 7, the combination that wherein said pH indicator comprises phenylazo compound.

9. according to the device described in claim 1 to 8 any one, wherein said device comprises cellulosic material.

10. a device, it comprises:

(a) fluid contact surfaces,

(b) relative nonfluid contact surface,

(c) the pH indicator that comprises covalent bond pH indicator therein, the pH of described pH indicator instruction fluid, Qi ZhongsuoState the change of the pH of fluid described in the color response of pH indicator and change; With,

(d) at least one is for the conduit towards described pH indicator guiding fluid.

11. devices according to claim 10, wherein said device has outer surface, and wherein pH indicator is positioned at instituteState outer surface or be positioned near described outer surface.

12. devices according to claim 11, wherein said device has in described fluid contact surfaces with described relativeNonfluid contact surface between the neighboring of extending, and wherein said outer surface is described neighboring.

13. according to claim 10 to the device described in 12 any one, and wherein said at least one conduit is indicated towards described pHDistrict's side direction guiding fluid.

14. according to claim 10 to the device described in 13 any one, and the change color in wherein said pH indicator is single 0.1The pH of bit interval can record under changing.

15. devices according to claim 14, the change color in wherein said pH indicator at about pH5 extremely approximatelyBetween pH10, can record.

16. devices according to claim 15, the change color in wherein said pH indicator is extremely large at about pH5.5Between about pH9.5, can record.

17. according to the device described in claim 15 or 16, and the change color in wherein said pH indicator is at about pH6.5To approximately recording between pH9.5.

18. according to claim 10 to the fluid dressing described in 17 any one, and wherein said pH indicator comprises phenylazoCompound.

19. devices according to claim 18, wherein said phenylazo compound is selected from the group of enumerating in table 1.

20. according to the device described in claim 18 or 19, the combination that wherein said pH indicator comprises phenylazo compound.

21. are used to indicate the preparation that comprises dyestuff of the pH of fluid, and described dyestuff comprises phenylazo compound, and institute whereinState the change of the pH of fluid described in the color response of phenylazo compound and change.

22. preparations according to claim 21, wherein said phenylazo compound is selected from the group of enumerating in table 1.

23. according to the preparation described in claim 21 or 22, the combination that wherein said dyestuff comprises phenylazo compound.

24. according to the preparation described in claim 21 to 23 any one, and the change color in wherein said pH indicator is single 0.1The pH of bit interval can record under changing.

25. preparations according to claim 24, the change color in wherein said pH indicator at about pH5 extremely approximatelyBetween pH10, can record.

26. preparations according to claim 25, the change color in wherein said pH indicator at about pH5 extremely approximatelyBetween pH9.5, can record.

27. according to the preparation described in claim 25 or 26, and the change color in wherein said pH indicator is at about pH6.5To approximately recording between pH9.5.

28. for monitoring the method for pH of fluid, said method comprising the steps of:

(a) provide and comprise the surperficial device that is configured to the described fluid of contact, it is fixed thereon that described surface has covalencyPH indicator, wherein said pH indicator with described fluid contact before there is the first color, and along with described fluidPH and change color,

(b) make described device and described fluid contact; With

(c) assess the color of described pH indicator.

29. comprise the device of the fluid contact surfaces with covalency pH marking tools fixed thereon, wherein said pH instructionInstrument with described fluid contact before there is the first color, and change color along with the pH of described fluid.

30. 1 kinds of devices, it comprises:

(a) pH marking tools, wherein said pH marking tools with fluid contact before there is the first color, and along with described streamThe pH of body and change color; With,

(b) for guide the conduit instrument of described fluid towards described pH marking tools.

The system of 31. pH for detection of fluid, it comprises that the device of claim 1-20 any one or claim 21-27 appointAt least one in the preparation of.