CN105334248A - Preparation method of electrochemical sensor for detection of homocysteine - Google Patents
Preparation method of electrochemical sensor for detection of homocysteine Download PDFInfo
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- CN105334248A CN105334248A CN201510802844.2A CN201510802844A CN105334248A CN 105334248 A CN105334248 A CN 105334248A CN 201510802844 A CN201510802844 A CN 201510802844A CN 105334248 A CN105334248 A CN 105334248A
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Abstract
The invention provides a preparation method of an electrochemical sensor for detection of homocysteine. The preparation method comprises preparation of a conducting polymer modified electrode and preparation of a poly(methyl violet) modified conducting polymer electrochemical sensor. The conducting polymer modified electrode is put into a phosphoric acid buffer solution containing 8*10<-5> mol/L poly(methyl violet) and subjected to electrolytic deposition for five minutes under +1.6V, then the scanned conducting polymer modified electrode is taken out and washed several times with absolute ethanol and secondary distilled water sequentially, and the poly(methyl violet) modified conducting polymer electrochemical sensor is prepared. The preparation method of the sensor is simple, and the sensitivity is high.
Description
Technical field
The present invention relates to a kind of electrochemical sensor, specifically a kind of electrochemical sensor preparation method detected fast for homocysteine.
Background technology
Homocysteine is also known as homocysteine, and be a kind of amino acid containing sulfydryl, in recent years, medical research has confirmed that the content of homocysteine in blood plasma and the raising of coronary heart disease and the cranial vascular disease incidence of disease have close contacting.Therefore, the pathological study of the relevant disease that the level of detection homocysteine is right is significant.
Establish the detection of various analysis for homocysteine at present, as photometric detection method, ultraviolet spectrophotometry, fluorescence method, spectrometry, electrochemical assay and enzyme immunoassay etc.But above-mentioned a lot of method also exists the shortcomings such as pre-service complexity, cost is high, sensitivity is low.Therefore timely, accurate, sensitive detection is carried out to homocysteine, become pressing issues urgently to be resolved hurrily.
Summary of the invention
The object of the invention is for prior art above shortcomings, the electrochemical sensor that a kind of quick, sensitive, easy homocysteine detects is provided.This sensor is based on the amplification of material to electrochemical detection signal, constructs the electrochemical sensor that advanced composite material (ACM) is modified.
The present invention relates to the preparation method of the electrochemical sensor detected for homocysteine, comprise the following steps:
The first step: the preparation of conducting polymer modified electrode:
First glass-carbon electrode is carried out ultrasonic cleaning in redistilled water after polishing; Then the glass-carbon electrode after cleaning puts into M polymer monomer, the water-soluble quinones sulfonate of M and graphene oxide solution, logical nitrogen is after 30 minutes, three-electrode system is adopted to carry out continuous current electro-deposition, then gained modified electrode is carried out electrochemical reduction, obtain conducting polymer/quinones sulfonated bodies/graphene oxide composite material modified electrode.
Second step: the preparation of the conducting polymer electrochemical sensor that poly-methyl violet is modified:
Conducting polymer modified electrode is placed in containing 8 × 10
-5in the phosphate buffer solution of mol/L methyl violet, electro-deposition five minutes under+1.6V, then take out the conducting polymer modified electrode through overscanning, rinse several times in turn successively with absolute ethyl alcohol and redistilled water, obtain the conducting polymer electrochemical sensor that poly-methyl violet is modified.
Described quinones sulfonate is the one of anthraquinone or phenanthrenequione.
Described M polymer monomer is the one in pyrroles, aniline, 3,4-rthylene dioxythiophene.
Specific embodiment
Embodiment is implemented under premised on technical solution of the present invention, give detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
Embodiment 1:
The glass-carbon electrode that pre-service is good is put into the phosphate buffer solution of pH=7.0, in the potential range of-0.2V ~+2.0V, carry out scan round process 10min with the sweep speed of 20mV/s.
Then electrode is put into the pyrrole monomer containing 0.1M, 0.0005M phenanthrenequione sulfonate, 0.5mg/ml graphene oxide water solution, logical N
2after 30min, adopt three-electrode system to carry out continuous current electro-deposition, applying electric current is 0.5mAcm
-2, the electricity of electro-deposition is 1.2 coulombs; Electrochemical reduction is carried out being placed in three-electrode system after being cleaned by obtained polypyrrole film, applying voltage is 1.1V, electrolytic solution is PBS buffer solution, repeatedly cleans can obtain polypyrrole/phenanthrenequione sulfonate/graphene composite material modified electrode after reaction with water;
Above-mentioned conducting polymer modified electrode is placed in containing 8 × 10
-5in the phosphate buffer solution of mol/L methyl violet, electro-deposition five minutes under+1.6V, then take out the conducting polymer modified electrode through overscanning, rinse several times in turn successively with absolute ethyl alcohol and redistilled water, obtain the conducting polymer electrochemical sensor that poly-methyl violet is modified.
Embodiment 2:
The glass-carbon electrode that pre-service is good is put into the phosphate buffered solution of pH=5.0, in the potential range of-0.2V ~+1.0V, carry out scan round process 15min with the sweep speed of 15mV/s;
Then electrode is put into pyrrole monomer, 0.0005M anthraquinone sulphonates, the 1.0mg/ml graphene oxide water solution containing 0.2M, logical N
2after 30min, adopt three-electrode system to carry out continuous current electro-deposition, applying electric current is 2mAcm
-2, the electricity of electro-deposition is 2.0 coulombs; Electrochemical reduction is carried out being placed in three-electrode system after being cleaned by obtained polypyrrole film, applying voltage is 1.2V, electrolytic solution is PBS buffer solution, repeatedly cleans can obtain polypyrrole/anthraquinone sulphonates/graphene composite material modified electrode after reaction with water;
Above-mentioned conducting polymer modified electrode is placed in containing 8 × 10
-5in the phosphate buffer solution of mol/L methyl violet, electro-deposition five minutes under+1.6V, then take out the conducting polymer modified electrode through overscanning, rinse several times in turn successively with absolute ethyl alcohol and redistilled water, obtain the conducting polymer electrochemical sensor that poly-methyl violet is modified.
Embodiment 3:
The glass-carbon electrode that pre-service is good is put into the phosphate buffered solution of pH=7.0, in the potential range of-0.2V ~+2.0V, carry out scan round process 50min with the sweep speed of 20mV/s;
Then electrode is put into containing aniline monomer, 0.1M phenanthrenequione sulfonate, the 1.0mg/ml graphene oxide water solution containing 0.05M, logical N
2after 30min, adopt three-electrode system to carry out continuous current electro-deposition, applying electric current is 1mAcm
-2, the electricity of electro-deposition is 1.6 coulombs; Electrochemical reduction is carried out being placed in three-electrode system after being cleaned by obtained polyaniline film, applying voltage is 1.0V, electrolytic solution is PBS buffer solution, repeatedly cleans can obtain polyaniline/phenanthrenequione sulfonate/graphene composite material modified electrode after reaction with water;
Above-mentioned conducting polymer modified electrode is placed in containing 8 × 10
-5in the phosphate buffer solution of mol/L methyl violet, electro-deposition five minutes under+1.6V, then take out the conducting polymer modified electrode through overscanning, rinse several times in turn successively with absolute ethyl alcohol and redistilled water, obtain the conducting polymer electrochemical sensor that poly-methyl violet is modified.
Claims (3)
1., for a preparation method for the electrochemical sensor of homocysteine detection, it is characterized in that, comprise the following steps:
The first step: the preparation of conducting polymer modified electrode:
First glass-carbon electrode is carried out ultrasonic cleaning in redistilled water after polishing; Then the glass-carbon electrode after cleaning puts into M polymer monomer, the water-soluble quinones sulfonate of M and graphene oxide solution, logical nitrogen is after 30 minutes, three-electrode system is adopted to carry out continuous current electro-deposition, then gained modified electrode is carried out electrochemical reduction, obtain conducting polymer/quinones sulfonated bodies/graphene oxide composite material modified electrode;
Second step: the preparation of the conducting polymer electrochemical sensor that poly-methyl violet is modified:
Conducting polymer modified electrode is placed in containing 8 × 10
-5in the phosphate buffer solution of mol/L methyl violet, electro-deposition five minutes under+1.6V, then take out the conducting polymer modified electrode through overscanning, rinse several times in turn successively with absolute ethyl alcohol and redistilled water, obtain the conducting polymer electrochemical sensor that poly-methyl violet is modified.
2. a kind of preparation method of electrochemical sensor detected for homocysteine according to claim 1, is characterized in that, described quinones sulfonate is the one in anthraquinone or phenanthrenequione.
3. a kind of preparation method of electrochemical sensor detected for homocysteine according to claim 1, it is characterized in that, described M polymer monomer is the one in pyrroles, aniline, 3,4-rthylene dioxythiophene.
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| CN201510802844.2A CN105334248B (en) | 2015-11-19 | 2015-11-19 | A kind of preparation method of electrochemical sensor for homocysteine detection |
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| CN201510802844.2A CN105334248B (en) | 2015-11-19 | 2015-11-19 | A kind of preparation method of electrochemical sensor for homocysteine detection |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106928434A (en) * | 2017-03-07 | 2017-07-07 | 常州大学 | A kind of method and its application by the tartrate ion synthesis of chiral conducting polymer that adulterates |
| CN110344077A (en) * | 2019-07-01 | 2019-10-18 | 吉林大学 | A method of by l-cysteine electrochemistry formated n-acetyl-L-cysteine |
| WO2020043026A1 (en) * | 2018-08-28 | 2020-03-05 | 长沙理工大学 | Method and sensor for detecting l-cystine |
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| US6652734B1 (en) * | 1999-03-16 | 2003-11-25 | Lifescan, Inc. | Sensor with improved shelf life |
| WO2005012871A2 (en) * | 2003-07-25 | 2005-02-10 | Dexcom, Inc. | Increasing bias for oxygen production in an electrode system |
| US20080164156A1 (en) * | 2007-01-05 | 2008-07-10 | Xueji Zhang | Enzymatic method for detecting a sulfur containing amino acid using an electrochemical sensor |
| WO2014112746A1 (en) * | 2013-01-21 | 2014-07-24 | 이손이엔엘(주) | Biosensor including microchip |
| CN104684477A (en) * | 2012-09-28 | 2015-06-03 | 德克斯康公司 | Zwitterion surface modifications for continuous sensors |
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2015
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US6652734B1 (en) * | 1999-03-16 | 2003-11-25 | Lifescan, Inc. | Sensor with improved shelf life |
| WO2005012871A2 (en) * | 2003-07-25 | 2005-02-10 | Dexcom, Inc. | Increasing bias for oxygen production in an electrode system |
| US20080164156A1 (en) * | 2007-01-05 | 2008-07-10 | Xueji Zhang | Enzymatic method for detecting a sulfur containing amino acid using an electrochemical sensor |
| CN104684477A (en) * | 2012-09-28 | 2015-06-03 | 德克斯康公司 | Zwitterion surface modifications for continuous sensors |
| WO2014112746A1 (en) * | 2013-01-21 | 2014-07-24 | 이손이엔엘(주) | Biosensor including microchip |
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| PALANISAMY KANNAN等: "Nitrogen doped graphene nanosheet supported platinum nanoparticles as high performance electrochemical homocysteine biosensors", 《JOURNAL OF MATERIALS CHEMISTRY B》 * |
| XIAOQIANG WANG等: "A Novel l-Cysteine Electrochemical Sensor Using Sulfonated Graphene-poly(3,4-Ethylenedioxythiophene) Composite Film Decorated with Gold Nanoparticles", 《ELECTROANALYSIS》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106928434A (en) * | 2017-03-07 | 2017-07-07 | 常州大学 | A kind of method and its application by the tartrate ion synthesis of chiral conducting polymer that adulterates |
| WO2020043026A1 (en) * | 2018-08-28 | 2020-03-05 | 长沙理工大学 | Method and sensor for detecting l-cystine |
| US11939625B2 (en) | 2018-08-28 | 2024-03-26 | Changsha University Of Science And Technology | Method and sensor for detecting L-cystine |
| CN110344077A (en) * | 2019-07-01 | 2019-10-18 | 吉林大学 | A method of by l-cysteine electrochemistry formated n-acetyl-L-cysteine |
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| CN105334248B (en) | 2018-01-16 |
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