CN105176796A - Vibrating equipment of cell culture fluid - Google Patents
Vibrating equipment of cell culture fluid Download PDFInfo
- Publication number
- CN105176796A CN105176796A CN201510622544.6A CN201510622544A CN105176796A CN 105176796 A CN105176796 A CN 105176796A CN 201510622544 A CN201510622544 A CN 201510622544A CN 105176796 A CN105176796 A CN 105176796A
- Authority
- CN
- China
- Prior art keywords
- cell culture
- culture fluid
- vibration device
- vibration
- vibrating diaphragm
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
Abstract
The invention discloses vibrating equipment of cell culture fluid. The vibrating equipment of the cell culture fluid comprises a signal generator and a loudspeaker which is electrically connected with the signal generator, the loudspeaker comprises a magnet, a basin frame and a vibrating membrane, and the vibrating membrane promotes the cell culture fluid to vibrate. According to the vibrating equipment of the cell culture fluid, vibration of the vibrating membrane of the loudspeaker is utilized to promote the cell culture fluid to vibrate, the sensitivity is higher, and the applicable frequency range is wider.
Description
Technical field
The present invention relates to a kind of vibration device of cell culture fluid.
Background technology
Existing cell analysis technology, is usually adopted tracer liquid resistance value to analyze liquid, is detected the growth conditions of cell by the change analyzing fluid impedance.But, the resistance value that same oscillation frequency possibility correspondence two is different.And if during an additional frequency, detect and easily make mistakes.And when impressed frequency is greater than liquid resonant frequency, may can't detect resistance value, measurement sensistivity is inadequate.
Summary of the invention
The technical problem to be solved in the present invention is, provides a kind of vibration device of cell culture fluid, and the vibration device sensitivity of this cell culture fluid is higher, and the scope of application is wider.
The present invention is for solving the problems of the technologies described above adopted technical scheme:
A kind of vibration device of cell culture fluid, the loud speaker that the vibration device of wherein said cell culture fluid comprises signal generator, is electrically connected with described signal generator, described loud speaker comprises magnet, frame and vibrating diaphragm, and described vibrating diaphragm impels described cell culture fluid to vibrate.
The invention has the beneficial effects as follows, utilize the vibration of membrane of shaking of loud speaker to vibrate to impel cell culture fluid, sensitivity is higher, and it is wider to be suitable for range of frequency.
Preferably, the vibration device of described cell culture fluid also comprises the accommodation box for cell culture fluid described in splendid attire, be arranged at the web member of described vibrating diaphragm, described web member support described accommodation box with by the vibration passing of described vibrating diaphragm to the cell culture fluid in described accommodation box.
Preferably, the signal frequency range that described signal generator sends is 20-6000 hertz.
Preferably, the vibration device of described cell culture fluid also comprises the accommodation box for cell culture fluid described in splendid attire, be arranged at the web member of described vibrating diaphragm, described web member to stretch in described accommodation box and contact described cell culture fluid with by the vibration passing of described vibrating diaphragm to the cell culture fluid in described accommodation box.
Preferably, described web member is provided with a plurality of vibration head, and described vibration head stretches in described cell culture fluid completely.
Preferably, the signal frequency range that described signal generator sends is 6000-20000 hertz.
Preferably, the signal frequency that described signal generator sends is 20-20000 hertz.
Accompanying drawing explanation
Above-described object of the present invention, technical scheme and beneficial effect by the detailed description that can realize specific embodiments of the invention below, can describe and clearly obtain simultaneously by reference to the accompanying drawings.
Identical label in accompanying drawing and specification sheets and symbol are for representing identical or equivalent element.
Fig. 1 shows the schematic diagram of the vibration device of the cell culture fluid that the embodiment of the present invention provides.
Wherein: 8. signal generator, 10. the first loud speaker, 12. first magnet, 14. first frame, 16. first vibrating diaphragms, 17. first accommodation boxes, 18. first web members, 19. second loud speakers, 20. second magnet, 21. second frame, 22. second vibrating diaphragms, 23. second accommodation boxes, 24. second web members, 26. vibration head.
Embodiment
As shown in Figure 1, a kind of vibration device of cell culture fluid, comprise signal generator 8, the first loud speaker 10, first loud speaker 10 of being electrically connected with signal generator 8 comprises the first magnet 12, first frame 14 and the first vibrating diaphragm 16, first vibrating diaphragm 16 impels cell culture fluid to vibrate.Signal generator 8 produces the signal of each frequency band, and the first vibrating diaphragm 16 being delivered to the first loud speaker 10, first loud speaker 10 vibrates, thus impels cell culture fluid to vibrate.
The invention has the beneficial effects as follows, utilize the vibration of the first vibrating diaphragm 16 of the first loud speaker 10 to vibrate to impel cell culture fluid, sensitivity is higher, thus accuracy of detection is higher, and it is wider to be suitable for range of frequency.
In this preferred embodiment, the vibration that the signal that can detect each frequency band of low frequency, intermediate frequency and high frequency produces is on the impact of cell culture fluid.Concrete, the signal frequency that signal generator 8 sends is 20-20000 hertz.
Further, the vibration device of cell culture fluid also comprises the first accommodation box 17 for splendid attire cell culture fluid, the first web member 18, first web member 18 being arranged at the first vibrating diaphragm 16 support the first accommodation box 17 with by the vibration passing of the first vibrating diaphragm 16 to the cell culture fluid in the first accommodation box 17.Now, the first loud speaker 10 is positioned at the below of cell culture fluid.The vibration produced for the signal detecting low and medium frequency is on the impact of cell culture fluid.Specifically, the signal frequency range that now signal generator 8 sends is 20-6000 hertz.
In addition, for making the frequency band of this equipment Inspection wider, the vibration device of cell culture fluid also comprises the second loud speaker 19, second loud speaker 19 comprises the second magnet 20, second frame 21 and the second vibrating diaphragm 22, the vibration device of cell culture fluid also comprises the second accommodation box 23 for splendid attire cell culture fluid, the second web member 24, second web member 24 being arranged at the second vibrating diaphragm 22 to stretch in the second accommodation box 23 and exposing cell nutrient solution with direct by the vibration passing of the second vibrating diaphragm 22 to the cell culture fluid in the second accommodation box 23.Concrete, the second web member 24 is provided with a plurality of vibration head 26, and vibration head 26 stretches in cell culture fluid completely.
Now, the second loud speaker 19 is positioned at the top of cell culture fluid.For detecting vibration that high-frequency signal produces to the impact of cell culture fluid.Specifically, the signal frequency range that now signal generator 8 sends is 6000-20000 hertz.Due to, the vibration that high-frequency signal produces is less, is Loss reducing, accurately detect that vibration that certain radio-frequency signal produces is on the impact of cell culture fluid, vibration head 26 is directly extend in cell culture fluid, makes sensitivity higher, detect more accurate.
Above embodiment is only the present invention's one, two kinds of embodiments wherein, and it describes comparatively concrete and detailed, but therefore can not be interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that for the person of ordinary skill of the art, under the prerequisite not departing from inventive concept of the present invention, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (7)
1. the vibration device of a cell culture fluid, it is characterized in that: the loud speaker that the vibration device of described cell culture fluid comprises signal generator, is electrically connected with described signal generator, described loud speaker comprises magnet, frame and vibrating diaphragm, and described vibrating diaphragm impels described cell culture fluid to vibrate.
2. the vibration device of cell culture fluid according to claim 1, it is characterized in that: the vibration device of described cell culture fluid also comprises the accommodation box for cell culture fluid described in splendid attire, be arranged at the web member of described vibrating diaphragm, described web member support described accommodation box with by the vibration passing of described vibrating diaphragm to the cell culture fluid in described accommodation box.
3. the vibration device of cell culture fluid according to claim 1, is characterized in that: the signal frequency range that described signal generator sends is 20-6000 hertz.
4. the vibration device of cell culture fluid according to claim 2, it is characterized in that: the vibration device of described cell culture fluid also comprises the accommodation box for cell culture fluid described in splendid attire, be arranged at the web member of described vibrating diaphragm, described web member to stretch in described accommodation box and contact described cell culture fluid with by the vibration passing of described vibrating diaphragm to the cell culture fluid in described accommodation box.
5. the vibration device of cell culture fluid according to claim 4, is characterized in that: described web member is provided with a plurality of vibration head, and described vibration head stretches in described cell culture fluid completely.
6. the vibration device of cell culture fluid according to claim 4, is characterized in that: the signal frequency range that described signal generator sends is 6000-20000 hertz.
7. the vibration device of cell culture fluid according to claim 1, is characterized in that: the signal frequency that described signal generator sends is 20-20000 hertz.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510622544.6A CN105176796B (en) | 2015-09-28 | 2015-09-28 | The vibratory equipment of cell culture fluid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510622544.6A CN105176796B (en) | 2015-09-28 | 2015-09-28 | The vibratory equipment of cell culture fluid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105176796A true CN105176796A (en) | 2015-12-23 |
| CN105176796B CN105176796B (en) | 2018-02-02 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510622544.6A Active CN105176796B (en) | 2015-09-28 | 2015-09-28 | The vibratory equipment of cell culture fluid |
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| Country | Link |
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| CN (1) | CN105176796B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1564713A (en) * | 2001-10-04 | 2005-01-12 | 塞弗德公司 | Apparatus and method for rapid disruption of cells or viruses |
| US20060024803A1 (en) * | 2001-02-19 | 2006-02-02 | Dornier Medtech Systems Gmbh | Method and device for ultrasonic inoculation of biological cell material |
| WO2006119932A1 (en) * | 2005-05-09 | 2006-11-16 | Orion Diagnostica Oy | Sonication of a medium |
| CN204145742U (en) * | 2014-09-05 | 2015-02-04 | 寻乌县爱馨泰电子商贸有限公司 | A kind of motor-operated horn with vibration transmission mechanism |
-
2015
- 2015-09-28 CN CN201510622544.6A patent/CN105176796B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060024803A1 (en) * | 2001-02-19 | 2006-02-02 | Dornier Medtech Systems Gmbh | Method and device for ultrasonic inoculation of biological cell material |
| CN1564713A (en) * | 2001-10-04 | 2005-01-12 | 塞弗德公司 | Apparatus and method for rapid disruption of cells or viruses |
| WO2006119932A1 (en) * | 2005-05-09 | 2006-11-16 | Orion Diagnostica Oy | Sonication of a medium |
| CN204145742U (en) * | 2014-09-05 | 2015-02-04 | 寻乌县爱馨泰电子商贸有限公司 | A kind of motor-operated horn with vibration transmission mechanism |
Non-Patent Citations (1)
| Title |
|---|
| 杜鹃等: "《电声技术与音响系统》", 31 May 2015 * |
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| Publication number | Publication date |
|---|---|
| CN105176796B (en) | 2018-02-02 |
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