CN104829341A - Seaweed oligosaccharide micro-ecology drip irrigation fertilizer and preparation method thereof - Google Patents
Seaweed oligosaccharide micro-ecology drip irrigation fertilizer and preparation method thereof Download PDFInfo
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Abstract
The present invention belongs to the technical field of seaweed fertilizers, and particularly relates to a seaweed oligosaccharide micro-ecology drip irrigation fertilizer, which comprises, by weight, 10-60% of a seaweed oligosaccharide solution, 10-50% of a molasses solution, 20-40% of a composite microorganism bacterial solution, and 10-40% of large, medium and trace elements, wherein crushed seaweed and water are mixed, a biological lyase is added to carry out enzymolysis so as to obtain a seaweed enzymolysis liquid, and extraction and pressure filtration are performed under an alkaline condition so as to obtain the seaweed oligosaccharide solution. The invention further provides a preparation method of the seaweed oligosaccharide micro-ecology drip irrigation fertilizer. According to the present invention, the effective component in the seaweed is substantially retained, the utilization rate of crops on the nutrients can be substantially improved, the preparation period is substantially shortened, the necessary organic matter and the trace elements can be supplemented, the composite microorganism bacterial solution is added, functions of disease resistance, stress tolerance, phosphorus dissolving, potassium dissolving, and soil physical structure increasing are provided, and various types of soil-borne diseases can be effectively prevented and controlled.
Description
Technical field
The invention belongs to seaweed fertilizer technical field, particularly relate to a kind of alga oligosaccharide Tiny ecosystem drip irrigation fertilizer and preparation method thereof.
Background technology
Seaweed fertilizer is natural fertilizer, and its fertilizer efficiency depends on nutritive ingredient contained in seaweed fertilizer, and the marine alga as fertilizer is generally large-scale economic class marine alga.The development experience of seaweed fertilizer three phases: rot marine alga → varec (powder) → seaweed extracted liquor.Core substance in seaweed fertilizer is pure natural Seaweed Extract, main raw material is selected from natural seaweed, through special biochemical process process, extract the essence substance in marine alga, retain natural radioactivity component, containing a large amount of non-nitrogen containing organism, the potassium that land plant is incomparable, calcium, magnesium, zinc, iodine etc. more than 40 plant mineral element and abundant VITAMIN, special in Sargassum polysaccharides specific in marine alga, alginic acid, highly unsaturated fatty acids and multiple natural phant growth regulator, there is very high biological activity, can the generation of the nonspecific activity factor in stimulating plant body, regulate Endogenous hormone balance.
Seaweed extracted liquor is the solvend extracted from large-scale fast-growing brown alga, is the mixture of plurality of active ingredients.Containing abundant growth regulatory substance and the antibiotics such as mineral substance, amino acid, vitamins and other nutritious components and phytokinin, growth hormone, phenols in marine alga liquid, be applied on food crop, fruit, vegetables, flowers and nursery stock, the growth of crop root and stem can be promoted, improve output, oil recovery enhancement, strengthens the ability of crop opposing poor environment.
Seaweed fertilizer raw material is selected from natural seaweed, has good affinity with terrestrial plant, nontoxic to people, animal, environmentally safe, can as organic food special fertilizer material.Seaweed fertilizer directly can make soil or by plant, soil be increased organic, activates the various beneficial microorganisms in soil, and these biologies can play a part catalyzer in plant-microbe metabolite circulation, and the biopotency of soil is increased.The metabolite of plants and soil microorganism can be plant provides more nutrient.Seaweed fertilizer is a kind of natural biological preparation, can work harmoniously with plant-soil ecosystem.Natural compounds such as the Sodium Alginate that it contains is natural soil amendment, the formation of soil aggregate can be promoted, improve soil internal void space, coordinate solid, liquid, gas proportions in soil, recover due to soil that over-burden and chemical pollution and the lac balance that loses, increase soil organisms vigor, promote the release of available nutrient, be conducive to root growth, improve the resistance of crop.
According to another statistics, just there is kind more than 100 in China only large-scale economical alga, is wherein no lack of the kind having high fertilizer efficiency, and resource is very abundant, and development and application seaweed fertilizer has a extensive future.
At present, along with the development of agricultural, seaweed fertilizer is combined with biological bacteria becomes a new research direction, microbial bacteria and organic substance combine, improve Soil structure and soil micro-ecosystem system, rehabilitating soil, plays global advantage, to set up optimum farmland's ecocycle system and crop alimentary combined system.But, the seaweed fertilizer of current interpolation biological bacteria, biologically active substance in seaweed fertilizer destroys more serious, and it is single to add bacterial classification, at raising utilization rate of fertilizer, improve Soil structure, improve Variation of Integrated Soil Fertility, promote that effective microorganisms body is formed, strengthen disease-resistant crops ability aspect effect undesirable.
The impact of production method on product biologically active substance and nutrient composition content of marine alga fertilizer is very big, the production of current seaweed fertilizer mainly adopts chemical hydrolysis, physics extraction method, enzymolysis process, biological fermentation process etc., adopt the highly basic hot environment of chemical hydrolysis can break the activity of meeting marine alga endogenous substance, physics extraction method is the object adopting the technique of high-temperature low-pressure cooling to reach the fragmentation of alginic cell wall, be better than chemical hydrolysis, but working condition is harsh, production cost is higher, industrialization transforms and there is larger difficulty, enzymolysis process and biological fermentation process are, by enzymolysis or fermenting process, the macromolecular substance forming marine alga is degraded into small molecules, water miscible material, remain the biologically active substance in marine alga and nutritive substance to greatest extent, and can be absorbed and used by plants, but also there is a lot of defects, one is that organism of fermentation bacterial classification is single, the enzyme system produced is single, be difficult to really to degrade the macromolecular substance of marine alga, affect the action effect of marine alga fertilizer, two is that biological fermentation process is prepared marine alga fertilizer and also existed and adopt fermentable bacterial classification to soil without any beneficial effect, simple adds production cost.
Summary of the invention
The object of the present invention is to provide a kind of alga oligosaccharide Tiny ecosystem drip irrigation fertilizer, greatly can retain the biologically active substance in marine alga and nutritive substance, add composite bacteria, easy to use, effective raising utilization rate of fertilizer, improves Soil structure, improves Variation of Integrated Soil Fertility, promote that effective microorganisms body is formed, strengthen disease-resistant crops ability.
The present invention realizes like this, a kind of alga oligosaccharide Tiny ecosystem drip irrigation fertilizer, comprise the component of following weight percent: the alga oligosaccharide solution of 10 ~ 60%, the liquid molasses of 10 ~ 50%, the composite microbial bacteria liquid of 20 ~ 40%, large, the middle and trace element of 10 ~ 40%, described alga oligosaccharide solution be by pulverize after marine alga mix with water, add biological lyase enzymolysis and obtain marine alga enzymolysis solution, extract in the basic conditions, press filtration and obtaining.
Improve as one, marine alga after described pulverizing and water are by 1:(4 ~ 7) weight ratio mix, add the biological lyase accounting for described marine alga and water mixed liquid 0.2 ~ 1% weight ratio, temperature be 35 ~ 75 DEG C, under pH value is 3 ~ 5 conditions, enzymolysis obtains marine alga enzymolysis solution in 12 ~ 24 hours, then pH value to 8.0 ~ 8.5 of marine alga enzymolysis solution are regulated, obtain described alga oligosaccharide solution again through press filtration, in described alga oligosaccharide solution, the weight content of alga oligosaccharide is more than or equal to 3.5%.
As improving further, in described marine alga enzymolysis solution, add potassium hydroxide solution adjust ph to 8.0 ~ 8.5.
As improving further, described biological lyase comprises Alginate Lyases and cellulase.
As improving further, described Alginate Lyases is endo-type mannuronic acid enzyme.
Improve as one, described composite microbial bacteria liquid is the one in bacillus bacterium liquid, EM bacterium liquid, actinomyces bacterium liquid, or is composited by wherein several.
Improve as one, described large, middle and trace element comprise in urea, potassium primary phosphate, saltpetre, chelating calcium, chelated magnesium, chelated iron, chelated zinc, chelated copper, Chelated Manganese, ammonium molybdate, boric acid, borax two or more.
Another object of the present invention is to the preparation method that a kind of alga oligosaccharide Tiny ecosystem drip irrigation fertilizer is provided, the effective constituent in natural seaweed can be retained greatly, improve crop greatly to the utilization ratio of nutrient, and organic matter, the trace element of needs can be supplemented, and can greatly shorten the cycle of preparing alga oligosaccharide.
A preparation method for alga oligosaccharide Tiny ecosystem drip irrigation fertilizer, described preparation method comprises the following steps:
(1) preparation of alga oligosaccharide solution: marine alga is dried and pulverizes, sea-tangle after pulverizing is added to the water to obtain mixed solution, the part by weight of sea-tangle and water is 1:(4 ~ 7), the biological lyase accounting for mixed solution weight 0.2 ~ 1% is added in the mixed solution of sea-tangle and water, under temperature is 35 ~ 75 DEG C of conditions, pH value is 3 ~ 5, enzymolysis 12 ~ 24 hours, obtain marine alga enzymolysis solution, in marine alga enzymolysis solution, add potassium hydroxide solution makes pH value be adjusted to 8 ~ 8.5, filtered by filtering equipment, filter residue, namely obtain alga oligosaccharide solution;
(2) cultivation of composite microbial bacteria liquid: by the bacterial classification in composite microbial bacteria liquid respectively fermentation culture obtain the single bacterium liquid of each bacterial classification, then that single bacterium liquid is in addition composite, obtained composite microbial bacteria liquid;
(3) obtained in step (1) alga oligosaccharide solution adds liquid molasses and large, middle and trace element, maintain the temperature at 70 DEG C and carry out chelatropic reaction, add composite microbial bacteria liquid obtained in step (2) after cooling, stir.
Improve as one, in the enzymolysis process of described step (1), add milk-acid bacteria.
Improve as one, in described step (2), the single bacterium liquid equal proportion of each bacterial classification is composite.
Owing to adopting technique scheme, the invention has the beneficial effects as follows:
Alga oligosaccharide Tiny ecosystem drip irrigation fertilizer provided by the invention, organic materials is mixed with composite microbial bacteria liquid, and add large, in, trace element and make organic fertilizer, wherein, alga oligosaccharide solution is through enzyme-squash techniqued, avoid and utilize pure chemistry method to be extracted by high temperature and high pressure method in the past, keep the activity of biologically active substance in marine alga, effective constituent in very big reservation marine alga, greatly can improve the utilization ratio of crop to nutrient, greatly shorten preparation cycle, and necessary organic matter and trace element can be supplemented, composite microbial bacteria liquid comprises multiple beneficial mushroom, microorganism carries out vital movement in soil, not only can improve the n-p-k element supply absorption level of plant, thus improve plant nutrition situation and outside increase yield, the growth of all kinds of plant hormone stimulating plant can also be produced, some pathogenic micro-organism of antagonism and suppress disease and pest, have disease-resistant, degeneration-resistant, phosphorus decomposing, potassium decomposing, increase the function of soil physics structure, and can effectively prevent and treat all kinds of soil-borne disease.
Alginate Lyases and cellulase is used in alga oligosaccharide in-solution digestion process, cellulase can enzymolysis marine alga cellulose tissue formed carbohydrate, alginates in Alginate Lyases degraded marine alga, and the type of the Substratspezifitaet of Alginate Lyases and alga oligosaccharide adapts, belong to endo-type mannuronic acid enzyme, be namely by extracting in marine organisms, there is specificity, compared with general enzyme, its enzymolysis speed and extraction yield improve greatly.Potassium hydroxide solution is used to regulate the pH value of marine alga enzymolysis solution, the main component of alga oligosaccharide is made to be potassium alginate, greatly increase Determination of Potassium in fertilizer, and the content of potassium alginate is more than 3.5% in alga oligosaccharide solution, preparation technology is simple, and can ensure the high-content of alga oligosaccharide in fertilizer.
Concrete hydrolysis temperature is for controlling between 35-75 degree, pH plays degradation speed obviously to be accelerated when being less than 5, when being less than 3, Lalgine can dewater precipitation, therefore pH controls between 3-5, enzymolysis speed is fast, greatly retain the effective constituent in marine alga simultaneously, and in enzymolysis process, in the mixed solution of marine alga and water, add milk-acid bacteria, because marine alga can carry out spontaneous fermentation in enzymolysis process, in fermentative degradation process, organic substance can produce some corrupt substances, fermented liquid can be made to become stench, milk-acid bacteria can produce lactobacillin, the generation of this phenomenon can be contained, and milk-acid bacteria can reduce the pH value in enzymolysis process thus promote the enzymolysis of marine alga, the pH value of marine alga enzymolysis solution is kept to be 8.0-8.5 in pressure-filtering process, avoid the activity of the biologically active substance in strong basicity environmental disruption marine alga enzymolysis solution, take into account again the solvability requirement of potassium alginate.
Liquid molasses adds honey by molasses culture medium, nitrogen, phosphorus, calcium, sodium, trace element, fermented bacterium is added in the substratum of the nutritive element compositions such as VITAMIN, carry out under aseptic condition in stainless steel fermentor tank closing fermentation, extract the substratum after fermentation through low temperature dewatering, molasses are formed after evaporation concentration, containing abundant organic composition, can with medium trace element generation chelatropic reaction, the present invention first liquid molasses is mixed with alga oligosaccharide solution then add large, in, trace element, comprising the medium trace element of Chelating state, and carry out chelatropic reaction, make the better effects if of medium trace element chelating, function nutrition group is more stable, not easily run off in soil, antagonism is there is between the medium trace element that effective prevention is added, dietetic alimentation utilization ratio can improve more than 30%.
Composite microbial bacteria liquid is bacillus bacterium liquid, EM bacterium liquid, one in actinomyces bacterium liquid, or be composited by wherein several, after huge microorganism species enters soil, the permeability of soil can be improved rapidly, the metabolite of probiotic bacterium in soil increases natural growth hormone to farm crop, have disease-resistant, degeneration-resistant, phosphorus decomposing, potassium decomposing, increase the function of soil physics structure, and can effectively prevent and treat all kinds of soil-borne disease, the single bacterium liquid of each bacterial classification is obtained after each bacterial classification ferments separately respectively, again fermentation is carried out in single bacterium liquid mixing and obtain complex micro organism fungicide, composite microbiological fertilizer prepared by the method effectively can keep bacterial activity, synergetic property between bacterial classification is strong, fertilizer efficiency is high.
The present invention not only by N P and K and trace element with the use of, and mineral element, microbial bacteria and organic substance are combined, play global advantage, set up optimum farmland's ecocycle system and crop alimentary combined system, make full use of Soil capabilities, reach and make crop yield increase income best effect.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearly understand, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
Embodiment one
A kind of alga oligosaccharide Tiny ecosystem drip irrigation fertilizer, comprises the component of following weight percent: the alga oligosaccharide solution of 20%, the liquid molasses of 20%, the composite microbial bacteria liquid of 40%, large, the middle and trace element of 20%.
Above-mentioned alga oligosaccharide solution be by pulverize after marine alga mix with the weight ratio of water by 1:4, add the biological lyase accounting for marine alga and water mixed liquid weight 0.5%, add the milk-acid bacteria accounting for marine alga and water mixed liquid weight 1%, temperature be 60 DEG C, under pH value is 3 ~ 5 conditions, enzymolysis obtains marine alga enzymolysis solution in 12 hours, then potassium hydroxide solution adjust ph to 8.0 ~ 8.5 are added in marine alga enzymolysis solution, carry out press filtration through pressure filter again and obtain alga oligosaccharide solution, in alga oligosaccharide solution, the main component of alga oligosaccharide is potassium alginate, and its weight content is 3.9%.
Wherein, biological lyase comprises Alginate Lyases and cellulase, and Alginate Lyases is endo-type mannuronic acid enzyme; Composite microbial bacteria liquid is composited by bacillus bacterium liquid and EM bacterium liquid; Described large, middle and trace element comprises urea, saltpetre, chelated iron, chelated zinc, boric acid.
The preparation method of above-mentioned alga oligosaccharide Tiny ecosystem drip irrigation fertilizer, comprises the following steps:
(1) preparation of alga oligosaccharide solution: sea-tangle is dried and pulverizes, sea-tangle after pulverizing is added to the water to obtain mixed solution, the part by weight of sea-tangle and water is 1:4, the biological lyase accounting for mixed solution weight 0.5% is added in the mixed solution of sea-tangle and water, under temperature is 60 DEG C of conditions, pH value is 3 ~ 5, enzymolysis 12 hours, and in enzymolysis process, add the milk-acid bacteria accounting for sea-tangle and water mixed liquid weight 1%, obtain marine alga enzymolysis solution, in marine alga enzymolysis solution, add potassium hydroxide solution makes pH value be adjusted to 8.0 ~ 8.5, filtered by filtering equipment, filter residue, namely alga oligosaccharide solution is obtained,
(2) cultivation of composite microbial bacteria liquid: be 35 DEG C in temperature, under air tight condition, aerobic Bacillus strain is inoculated respectively in activation medium, activation culture 10 ~ 12 hours, then cultured bacterial classification is fermented respectively in production fermention medium and expand numerous 24 ~ 72 hours, expand in numerous process in activation culture and fermentation and constantly shake or stir, obtain various genus bacillus bacterium liquid; EM bacterial classification is connected in the substratum of high-temperature sterilization, anaerobically fermenting 24 ~ 48 hours, obtains EM bacterium liquid, then genus bacillus bacterium liquid and EM bacterium liquid are mixed to get composite microbial bacteria liquid according to the ratio of weight ratio 1:1;
(3) obtained in step (1) alga oligosaccharide solution adds liquid molasses and large, middle and trace element, maintain the temperature at 70 DEG C and carry out chelatropic reaction, chelatropic reaction 45 minutes, composite microbial bacteria liquid obtained in step (2) is added in cooling afterwards, stirs.
Embodiment two
A kind of alga oligosaccharide Tiny ecosystem drip irrigation fertilizer, comprises the component of following weight percent: the alga oligosaccharide solution of 10%, the liquid molasses of 30%, the composite microbial bacteria liquid of 30%, large, the middle and trace element of 30%.
Above-mentioned alga oligosaccharide solution be by pulverize after sargassun mix with the weight ratio of water by 1:4, add the biological lyase accounting for sargassun and water mixed liquid 0.2% weight ratio, it is 35 DEG C in temperature, pH value is under 3 ~ 5 conditions, enzymolysis obtains marine alga enzymolysis solution in 16 hours, and in enzymolysis process, add the milk-acid bacteria accounting for marine alga and water mixed liquid weight 0.5%, then potassium hydroxide solution adjust ph to 8.0 ~ 8.5 are added in marine alga enzymolysis solution, carry out press filtration through pressure filter again and obtain alga oligosaccharide solution, in alga oligosaccharide solution, the main component of alga oligosaccharide is potassium alginate, its weight content is 3.8%.
Wherein, biological lyase comprises Alginate Lyases and cellulase, and Alginate Lyases is endo-type mannuronic acid enzyme; Composite microbial bacteria liquid is composited by bacillus bacterium liquid and actinomycetes bacterium liquid; Described large, middle and trace element comprises urea, potassium primary phosphate, saltpetre, chelated iron, chelated zinc, Chelated Manganese, ammonium molybdate, boric acid.
The preparation method of above-mentioned alga oligosaccharide Tiny ecosystem drip irrigation fertilizer, comprises the following steps:
(1) preparation of alga oligosaccharide solution: sargassun is dried and pulverizes, sargassun after pulverizing is added to the water to obtain mixed solution, the part by weight of sargassun and water is 1:4, the biological lyase accounting for mixed solution weight 0.2% is added in the mixed solution of sargassun and water, under temperature is 35 DEG C of conditions, pH value is 3 ~ 5, enzymolysis 16 hours, and in enzymolysis process, add the milk-acid bacteria accounting for marine alga and water mixed liquid weight 0.5%, obtain marine alga enzymolysis solution, in marine alga enzymolysis solution, add potassium hydroxide solution makes pH value be adjusted to 8.0 ~ 8.5, filtered by filtering equipment, filter residue, namely alga oligosaccharide solution is obtained,
(2) cultivation of composite microbial bacteria liquid: be 35 DEG C in temperature, under air tight condition, aerobic Bacillus strain is inoculated respectively in activation medium, activation culture 10 ~ 12 hours, then cultured bacterial classification is fermented respectively in production fermention medium and expand numerous 24 ~ 72 hours, expand in numerous process in activation culture and fermentation and constantly shake or stir, obtain genus bacillus bacterium liquid; Actinomyces strain inoculation is accounted for 2.5wt% to W-Gum, soybean cake powder accounts for 3.5 ~ 4wt%, corn steep liquor accounts for 1.5wt%, thiamines accounts for 1wt%, soybean oil accounts in the liquid nutrient medium of 1%, is 28 DEG C, between pH value 6.0 ~ 7.5 in temperature, cultivate 24 hours i.e. obtained actinomyces bacterium liquid, then genus bacillus bacterium liquid and actinomyces bacterium liquid are mixed to get composite microbial bacteria liquid according to the ratio of weight ratio 1:1;
(3) obtained in step (1) alga oligosaccharide solution adds liquid molasses and large, middle and trace element, maintain the temperature at 70 DEG C and carry out chelatropic reaction, chelatropic reaction cooled after 45 minutes, add composite microbial bacteria liquid obtained in step (2), stir.
Embodiment three
A kind of alga oligosaccharide Tiny ecosystem drip irrigation fertilizer, comprises the component of following weight percent: the alga oligosaccharide solution of 60%, the liquid molasses of 10%, the composite microbial bacteria liquid of 20%, large, the middle and trace element of 10%.
Above-mentioned alga oligosaccharide solution be by pulverize after sea-tangle mix with the weight ratio of water by 1:7, add the biological lyase accounting for sea-tangle and water mixed liquid 1% weight ratio, it is 75 DEG C in temperature, pH value is under 3 ~ 5 conditions, enzymolysis obtains marine alga enzymolysis solution in 24 hours, and in enzymolysis process, add the milk-acid bacteria accounting for marine alga and water mixed liquid weight 0.8%, then potassium hydroxide solution adjust ph to 8.0 ~ 8.5 are added in marine alga enzymolysis solution, carry out press filtration through pressure filter again and obtain alga oligosaccharide solution, in alga oligosaccharide solution, the main component of alga oligosaccharide is potassium alginate, its weight percent is 3.7%.
Wherein, biological lyase comprises Alginate Lyases and cellulase, and Alginate Lyases is endo-type mannuronic acid enzyme; Composite microbial bacteria liquid is composited by EM bacterium liquid and actinomyces bacterium liquid; Described large, middle and trace element comprise in urea, potassium primary phosphate, saltpetre, chelating calcium, chelated magnesium, chelated iron, chelated zinc, chelated copper, Chelated Manganese, ammonium molybdate, boric acid, borax two or more.
The preparation method of above-mentioned alga oligosaccharide Tiny ecosystem drip irrigation fertilizer, comprises the following steps:
(1) preparation of alga oligosaccharide solution: sea-tangle is dried and pulverizes, sea-tangle after pulverizing is added to the water to obtain mixed solution, the part by weight of sea-tangle and water is 1:7, the biological lyase accounting for mixed solution weight 1% is added in the mixed solution of sea-tangle and water, under temperature is 75 DEG C of conditions, pH value is 3 ~ 5, enzymolysis 24 hours, and in enzymolysis process, add the milk-acid bacteria accounting for sea-tangle and water mixed liquid weight 0.8%, obtain marine alga enzymolysis solution, in marine alga enzymolysis solution, add potassium hydroxide solution makes pH value be adjusted to 8.0 ~ 8.5, filtered by filtering equipment, filter residue, namely alga oligosaccharide solution is obtained,
(2) cultivation of composite microbial bacteria liquid: EM bacterial classification is connected in the substratum of high-temperature sterilization, anaerobically fermenting 24 ~ 48 hours, obtains EM bacterium liquid; Actinomyces strain inoculation is accounted for 2.5wt% to W-Gum, soybean cake powder accounts for 3.5 ~ 4wt%, corn steep liquor accounts for 1.5wt%, thiamines accounts for 1wt%, soybean oil accounts in the liquid nutrient medium of 1%, is 28 DEG C, between pH value 6.0 ~ 7.5 in temperature, cultivate 24 hours i.e. obtained actinomyces bacterium liquid, then EM bacterium liquid and actinomyces bacterium liquid are mixed to get composite microbial bacteria liquid according to the ratio of weight ratio 1:1;
(3) obtained in step (1) alga oligosaccharide solution adds liquid molasses and large, middle and trace element, maintain the temperature at 70 DEG C and carry out chelatropic reaction, chelatropic reaction cooled after 1 hour, add composite microbial bacteria liquid obtained in step (2), stir.
Embodiment four
A kind of alga oligosaccharide Tiny ecosystem drip irrigation fertilizer, comprises the component of following weight percent: the alga oligosaccharide solution of 20%, the liquid molasses of 50%, the composite microbial bacteria liquid of 20%, large, the middle and trace element of 10%.
Above-mentioned alga oligosaccharide solution be by pulverize after sargassun mix with the weight ratio of water by 1:6, add the biological lyase accounting for sargassun and water mixed liquid 0.8% weight ratio, it is 50 DEG C in temperature, pH value is under 3 ~ 5 conditions, enzymolysis obtains marine alga enzymolysis solution in 18 hours, and in enzymolysis process, add the milk-acid bacteria accounting for marine alga and water mixed liquid weight 1.2%, then potassium hydroxide solution adjust ph to 8.0 ~ 8.5 are added in marine alga enzymolysis solution, carry out press filtration through pressure filter again and obtain alga oligosaccharide solution, in alga oligosaccharide solution, the main component of alga oligosaccharide is potassium alginate, its weight percent is 3.6%.
Wherein, biological lyase comprises Alginate Lyases and cellulase, and Alginate Lyases is endo-type mannuronic acid enzyme; Composite microbial bacteria liquid is composited by bacillus bacterium liquid, EM bacterium liquid, actinomyces bacterium liquid; Described large, middle and trace element comprises urea, potassium primary phosphate, saltpetre, chelating calcium, chelated magnesium, chelated iron, chelated zinc, chelated copper, Chelated Manganese, ammonium molybdate, boric acid, borax.
The preparation method of above-mentioned alga oligosaccharide Tiny ecosystem drip irrigation fertilizer, comprises the following steps:
(1) preparation of alga oligosaccharide solution: sargassun is dried and pulverizes, sargassun after pulverizing is added to the water to obtain mixed solution, the part by weight of sargassun and water is 1:6, the biological lyase accounting for mixed solution weight 0.8% is added in the mixed solution of sargassun and water, under temperature is 50 DEG C of conditions, pH value is 3 ~ 5, enzymolysis 18 hours, and in enzymolysis process, add the milk-acid bacteria accounting for marine alga and water mixed liquid weight 1.2%, obtain marine alga enzymolysis solution, in marine alga enzymolysis solution, add potassium hydroxide solution makes pH value be adjusted to 8.0 ~ 8.5, filtered by filtering equipment, filter residue, namely alga oligosaccharide solution is obtained,
(2) cultivation of composite microbial bacteria liquid: be 35 DEG C in temperature, under air tight condition, aerobic Bacillus strain is inoculated respectively in activation medium, activation culture 10 ~ 12 hours, then cultured bacterial classification is fermented respectively in production fermention medium and expand numerous 24 ~ 72 hours, expand in numerous process in activation culture and fermentation and constantly shake or stir, obtain genus bacillus bacterium liquid; EM bacterial classification is connected in the substratum of high-temperature sterilization, anaerobically fermenting 24 ~ 48 hours, obtains EM bacterium liquid; Actinomyces strain inoculation is accounted for 2.5wt% to W-Gum, soybean cake powder accounts for 3.5 ~ 4wt%, corn steep liquor accounts for 1.5wt%, thiamines accounts for 1wt%, soybean oil accounts in the liquid nutrient medium of 1wt%, is about 28 DEG C in temperature, and pH is 6.0 ~ 7.5, cultivate 24 hours i.e. obtained actinomyces bacterium liquid, respectively genus bacillus bacterium liquid, EM bacterium liquid and actinomyces bacterium liquid are mixed to get composite microbial bacteria liquid according to the ratio of weight ratio 1:1:1;
(3) obtained in step (1) alga oligosaccharide solution adds liquid molasses and large, middle and trace element, maintain the temperature at 70 DEG C and carry out chelatropic reaction, chelatropic reaction cooled after 1 hour, add composite microbial bacteria liquid obtained in step (2), stir.
Embodiment five
A kind of alga oligosaccharide Tiny ecosystem drip irrigation fertilizer, comprises the component of following weight percent: the alga oligosaccharide solution of 20%, the liquid molasses of 20%, the composite microbial bacteria liquid of 20%, large, the middle and trace element of 40%.
Above-mentioned alga oligosaccharide solution be by pulverize after sea-tangle mix with the weight ratio of water by 1:5, add the biological lyase accounting for sea-tangle and water mixed liquid 0.4% weight ratio, it is 50 DEG C in temperature, pH value is under 3 ~ 5 conditions, enzymolysis obtains marine alga enzymolysis solution in 16 hours, and in enzymolysis process, add the milk-acid bacteria accounting for marine alga and water mixed liquid weight 1.5%, then potassium hydroxide solution adjust ph to 8.0 ~ 8.5 are added in marine alga enzymolysis solution, carry out press filtration through pressure filter again and obtain alga oligosaccharide solution, in alga oligosaccharide solution, the main component of alga oligosaccharide is potassium alginate, its weight content is 3.7%.
Wherein, biological lyase comprises Alginate Lyases and cellulase, and Alginate Lyases is endo-type mannuronic acid enzyme; Composite microbial bacteria liquid is EM bacterium liquid; Described large, middle and trace element comprises urea, potassium primary phosphate, saltpetre, chelating calcium, chelated magnesium, chelated iron, chelated zinc, chelated copper, Chelated Manganese, ammonium molybdate, boric acid, borax.
The preparation method of above-mentioned alga oligosaccharide Tiny ecosystem drip irrigation fertilizer, comprises the following steps:
(1) preparation of alga oligosaccharide solution: marine alga is dried and pulverizes, sea-tangle after pulverizing is added to the water to obtain mixed solution, the part by weight of sea-tangle and water is 1:6, the biological lyase accounting for mixed solution weight 0.4% is added in the mixed solution of sea-tangle and water, under temperature is 50 DEG C of conditions, pH value is 3 ~ 5, enzymolysis 16 hours, and in enzymolysis process, add the milk-acid bacteria accounting for marine alga and water mixed liquid weight 1.5%, obtain marine alga enzymolysis solution, in marine alga enzymolysis solution, add potassium hydroxide solution makes pH value be adjusted to 8.0 ~ 8.5, filtered by filtering equipment, filter residue, namely alga oligosaccharide solution is obtained,
(2) cultivation of composite microbial bacteria liquid: be 35 DEG C, under air tight condition in temperature, is connected in the substratum of high-temperature sterilization by EM bacterial classification, anaerobically fermenting 24 ~ 48 hours, obtains EM bacterium liquid;
(3) obtained in step (1) alga oligosaccharide solution adds liquid molasses and large, middle and trace element, maintain the temperature at 70 DEG C and carry out chelatropic reaction, chelatropic reaction cooled after 80 minutes, add composite microbial bacteria liquid obtained in step (2), stir.
The alga oligosaccharide Tiny ecosystem drip irrigation fertilizer obtained by embodiment one, uses 1 ~ 10KG for every mu, dilutes 300 ~ 500 times of drip irrigations or punching is executed, and test at Experimental Base, compared with peasant's conventional fertilizer application, comparing result is as following table:
By using crops such as cucumber, eggplant, apple, oranges and tangerines and grapes, can find out, compared with peasant's conventional fertilizer application, crop yield more than 15% can be improved, enhance resistance against diseases simultaneously, improve product quality, visible, alga oligosaccharide Tiny ecosystem drip irrigation fertilizer provided by the invention, significantly improves the utilization ratio of Nitrogen In Soils phosphorus potassium and medium trace element, effectively reduces planting cost.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.
Claims (10)
1. an alga oligosaccharide Tiny ecosystem drip irrigation fertilizer, it is characterized in that, comprise the component of following weight percent: the alga oligosaccharide solution of 10 ~ 60%, the liquid molasses of 10 ~ 50%, the composite microbial bacteria liquid of 20 ~ 40%, large, the middle and trace element of 10 ~ 40%, described alga oligosaccharide solution be by pulverize after marine alga mix with water, add biological lyase enzymolysis and obtain marine alga enzymolysis solution, extract in the basic conditions, press filtration and obtaining.
2. alga oligosaccharide Tiny ecosystem drip irrigation fertilizer according to claim 1, it is characterized in that, marine alga after described pulverizing and water are by 1:(4 ~ 7) weight ratio mix, add the biological lyase accounting for described marine alga and water mixed liquid 0.2 ~ 1% weight ratio, temperature be 35 ~ 75 DEG C, under pH value is 3 ~ 5 conditions, enzymolysis obtains marine alga enzymolysis solution in 12 ~ 24 hours, then pH value to 8.0 ~ 8.5 of marine alga enzymolysis solution are regulated, obtain described alga oligosaccharide solution again through press filtration, in described alga oligosaccharide solution, the weight content of alga oligosaccharide is more than or equal to 3.5%.
3. alga oligosaccharide Tiny ecosystem drip irrigation fertilizer according to claim 2, is characterized in that, adds potassium hydroxide solution adjust ph to 8.0 ~ 8.5 in described marine alga enzymolysis solution.
4. alga oligosaccharide Tiny ecosystem drip irrigation fertilizer according to claim 3, is characterized in that, described biological lyase comprises Alginate Lyases and cellulase.
5. alga oligosaccharide Tiny ecosystem drip irrigation fertilizer according to claim 4, is characterized in that, described Alginate Lyases is endo-type mannuronic acid enzyme.
6. alga oligosaccharide Tiny ecosystem drip irrigation fertilizer according to claim 1, is characterized in that, described composite microbial bacteria liquid is the one in bacillus bacterium liquid, EM bacterium liquid, actinomyces bacterium liquid, or is composited by wherein several.
7. alga oligosaccharide Tiny ecosystem drip irrigation fertilizer according to claim 1, it is characterized in that, described large, middle and trace element comprise in urea, potassium primary phosphate, saltpetre, chelating calcium, chelated magnesium, chelated iron, chelated zinc, chelated copper, Chelated Manganese, ammonium molybdate, boric acid, borax two or more.
8. a preparation method for alga oligosaccharide Tiny ecosystem drip irrigation fertilizer as claimed in claim 1, is characterized in that, comprise the following steps:
(1) preparation of alga oligosaccharide solution: marine alga is dried and pulverizes, sea-tangle after pulverizing is added to the water to obtain mixed solution, the part by weight of sea-tangle and water is 1:(4 ~ 7), the biological lyase accounting for mixed solution weight 0.2 ~ 1% is added in the mixed solution of sea-tangle and water, under temperature is 35 ~ 75 DEG C of conditions, pH value is 3 ~ 5, enzymolysis 12 ~ 24 hours, obtain marine alga enzymolysis solution, in marine alga enzymolysis solution, add potassium hydroxide solution makes pH value be adjusted to 8 ~ 8.5, filtered by filtering equipment, filter residue, namely obtain alga oligosaccharide solution;
(2) cultivation of composite microbial bacteria liquid: by the bacterial classification in composite microbial bacteria liquid respectively fermentation culture obtain the single bacterium liquid of each bacterial classification, then that single bacterium liquid is in addition composite, obtained composite microbial bacteria liquid;
(3) obtained in step (1) alga oligosaccharide solution adds liquid molasses and large, middle and trace element, maintain the temperature at 70 DEG C and carry out chelatropic reaction, add composite microbial bacteria liquid obtained in step (2) after cooling, stir.
9. the preparation method of alga oligosaccharide Tiny ecosystem drip irrigation fertilizer according to claim 8, is characterized in that, adds milk-acid bacteria in the enzymolysis process of described step (1).
10. the preparation method of alga oligosaccharide Tiny ecosystem drip irrigation fertilizer according to claim 8, is characterized in that, in described step (2), the single bacterium liquid equal proportion of each bacterial classification is composite.
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