CN104825233B - Experimental device for frog heart perfusion - Google Patents
Experimental device for frog heart perfusion Download PDFInfo
- Publication number
- CN104825233B CN104825233B CN201510241994.0A CN201510241994A CN104825233B CN 104825233 B CN104825233 B CN 104825233B CN 201510241994 A CN201510241994 A CN 201510241994A CN 104825233 B CN104825233 B CN 104825233B
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- CN
- China
- Prior art keywords
- plug
- frog heart
- artery
- arterial
- frog
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
Abstract
The invention relates to an experimental device for medical function and physiology, in particular to an experimental device for frog heart perfusion. The experimental device comprises a buffer tube, a three-way buffer artery tube, an artery plug, an artery cannula, a quantitative drop device, a venous cannula and a venous plug, wherein an opening is formed in the top of the buffer tube; the three-way buffer artery tube is connected with the buffer tube; the artery plug is connected with one branch tube of the three-way buffer artery tube; the artery cannula is connected with the other branch tube of the three-way buffer artery tube; the quantitative drop device is connected with the artery cannula; the venous cannula is arranged below the quantitative drop device; the venous plug is connected with the venous cannula; the artery plug is inserted into an aorta of a frog heart; the venous plug is inserted into a postcaval vein of the frog heart. According to the experimental device for the frog heart perfusion, disclosed by the invention, the buffer action for perfused liquid ejected in the blood ejection process of the frog heart is realized, uninterrupted dropwise outflow of the perfused liquid can be kept, the perfused liquid can flow at the systole and diastole of the heart, and quantitative record of liquid can be realized by the quantitative drop device.
Description
Technical field
The present invention relates to Medical Function, Physiology Experiment device, and in particular to a kind of frog heart perfusion experiment device.
Background technology
Perfused isolated heart is one of important means of research cardiac function, is widely used in medical science, biology, pharmacy etc.
In teaching and scientific research work.The activity of heart, the easypro contracting work(of research heart can intuitively be observed using Perfused isolated heart system
Energy, heart rate and the rhythm and pace of moving things change, the impact to cardiac function such as load and various humoral factors in front and back.Carried out in vitro using frog heart
Cardiac perfusion is a kind of more conventional model, stable because of it, economical, widely used by people always.Carry out isolated heart filling
Stream experiment, it is necessary first to ensure heart long period survival in vitro, then carries out perfusion and applies experiment according to experiment purpose
Factor.The operations such as intubation, the blood flow flushing before either heart is in vitro, or replace blood to enter with artificial solution after heart is in vitro
Row perfusion, makes heart departing from physiological neuro humor environment.Therefore, make the isolated heart long period survive, and remain good
Good state is particularly important for completing to test and obtaining reliability experimental data.
Injection is in the form of a column in paradoxical expansion infusion liquid in traditional experiment device, and relaxing period is penetrated because of no pressure without infusion liquid
Go out.Primitive apparatus is without separator tube, it is impossible to ensure that relaxing period infusion liquid is projected, and systole is then projected into column, is not easy to the frog heart and is penetrated
Go out the quantization of liquid.
The content of the invention
An object of the present invention is to solve to ensure that relaxing period infusion liquid is projected, being not easy to frog heart liquid jet
A kind of quantification problem, there is provided frog heart perfusion experiment device, the infusion liquid projected during blood is penetrated to the frog heart and plays cushioning effect, can
Keep the continual drop-wise of infusion liquid to flow out, have infusion liquid to flow out in paradoxical expansion and relaxing period, by apparatus for counting drops energy
Realize that liquid quantifies record.
In order to reach above-mentioned technique effect, technical scheme includes：
One kind improves frog heart perfusion experiment device, including tremulous pulse plug, arterial cannulation, with the amount that the arterial cannulation connects
The vein plug for change drip apparatus, the venous cannulation being arranged on below the quantization drip apparatus, connecting with the venous cannulation, it is described
On the aorta of the tremulous pulse plug insertion frog heart, on the posterior vena cava of the vein plug insertion frog heart, it is characterised in that including top
Portion is provided with the separator tube of opening, one that arterial, threeway buffer arterial is buffered with the threeway of separator tube connection
Another arm connection arterial cannulation of pipe connection tremulous pulse plug, threeway buffering arterial.
The quantization drip apparatus include note dropper, note dropping electrode and note drop guide line.
The quantization drip apparatus recording mode is that 16 drops are calculated as 1ml.
Beneficial effect of the present invention includes：
1st, frog heart perfusion experiment device of the present invention, the infusion liquid projected during blood is penetrated to the frog heart and plays cushioning effect, can
Keep the continual drop-wise of infusion liquid to flow out, have infusion liquid to flow out in paradoxical expansion and relaxing period, by quantifying drip apparatus
Can realize that liquid quantifies record.
2nd, frog heart perfusion experiment device of the present invention, can to a certain degree improve the temperature of circulating liquid, keep frog heart specimen
Activity.
3rd, frog heart perfusion experiment device of the present invention, device is simple, easily operation, more accurately measures frog cardiac output, master
The physiological datas such as tremulous pulse blood volume, improve accuracy rate, the success rate of experiment.
Description of the drawings
Fig. 1 show frog heart perfusion experiment device schematic diagram of the present invention.
Fig. 2 show partial enlarged drawing at the A of frog heart perfusion experiment device Fig. 1 of the present invention.
Diagram：1- separator tubes, 2- threeways buffering arterial, 3- tremulous pulse plugs, 4- arterial cannulations, 5- quantify drip apparatus, 51-
Note dropper, 52- note dropping electrodes, 53- note drop guide lines, 6- venous cannulations, 7- vein plugs, 8- biological functional systems.
Specific embodiment
The specific embodiment of the invention is described in detail below in conjunction with concrete accompanying drawing.It should be noted that in following embodiments
The technical characteristic of description or the combination of technical characteristic are not construed as isolated, and they can be mutually combined so as to reach
To superior technique effect.In the accompanying drawing of following embodiments, the identical label that each accompanying drawing occurs represent identical feature or
Person's part, in can be applicable to different embodiments.
Embodiment：
A kind of frog heart perfusion experiment device schematic diagram of the invention as shown in Figure 1.A kind of frog heart perfusion experiment device, including top
Portion is provided with the separator tube 1 of opening, buffers arterial 2 with the threeway of the connection of the separator tube 1, buffers arterial with the threeway
The tremulous pulse plug 3 of 2 arm connection, buffer with the threeway arterial 2 the connection of another arm arterial cannulation 4, same
The connection of the arterial cannulation 4 quantify drip apparatus 5, be arranged on the venous cannulation 6 of quantization drip apparatus 5 lower section, with described quiet
The vein plug 7 of the connection of arteries and veins intubation 6, on the aorta of the insertion of tremulous pulse plug 3 frog heart, the vein plug 7 inserts the frog heart
Posterior vena cava on.
As shown in Fig. 2 the quantization drip apparatus 5 include note dropper 51, note dropping electrode 52 and note drop guide line 53.
Quantization drip apparatus 5 recording mode is that 16 drops are calculated as 1ml.
When preparing perfusion experiment, experimental provision is connected, with probe frog spinal cord and brain are destroyed, dorsal positions are fixed on frog board
On.The thoracic cavity exposure frog heart is opened, pericardium is cut off, first ringer solution is filled in the separator tube 1 of device, the tremulous pulse in device is inserted
In the insertion frog aorta of pipe 3, with line ligation is fixed；The frog heart is turned over upwards, caval vein is exposed, by the venous cannulation 6 in device
Ligation in insertion caval vein is fixed.Blood vessel is cut off with operating scissorss, the frog heart is proposed from thoracic cavity, be fixed on universal stand.Will
The experimental provision of the present invention connects with biological functional system 8.There are the quantization drip apparatus 5 of note drop in a device.Quantify drop
Device 5 is made up of note dropper 51, note dropping electrode 52 and note drop guide line 53.Quantify drip apparatus 5 and be placed in just going up for venous cannulation 6
Side.Quantify drip apparatus 5 and biological functional system 8, biological function experiment are connected by note dropping electrode 52 and note drop guide line 53
System 8 is connected with computer, when there is drips, by remembering that dropping electrode 52 senses, and induced signal is delivered to into biological machine
Energy experimental system 8, just can write down on computers, realize that quantization is acted on such that it is able to determine frog cardiac output.
Separator tube 1 adds infusion based on buffering and energy conversion in separator tube 1.The connection threeway buffering of separator tube 1
Arterial 2, the arm connection tremulous pulse plug 3, threeway of the threeway buffering arterial 2 buffers the another of arterial 2
Arm connects arterial cannulation 4, and after the insertion aorta of tremulous pulse plug 3, the threeway buffering Jing distributing T-pipes of arterial 2 make infusion liquid one
Tremulous pulse plug 3 is partially into, another part Jing arterial cannulations 4 are entered and quantify drip apparatus 5, and quantified drip apparatus 5 carry out quantization note
Record, the drop of note 16 is 1ml, and then infusion liquid Jing venous cannulation 6 arrives 7 times hearts of vein plug.Formed extracorporeal circulation in the body frog
Heart perfusion device.
A kind of frog heart perfusion experiment device of the present invention, the infusion liquid projected during blood is penetrated to the frog heart and plays cushioning effect,
The continual drop-wise of infusion liquid can be kept to flow out, have infusion liquid to flow out in paradoxical expansion and relaxing period, by quantifying drop dress
Putting can realize that liquid quantifies record.
Above-mentioned detailed description is illustrating for the possible embodiments for invention, and the embodiment simultaneously is not used to limit this
Bright the scope of the claims, all equivalence enforcements or change without departing from the present invention, should be contained in the scope of the claims of the present invention.
In addition, those skilled in the art can also the claims in the present invention scope of disclosure and spirit in do other forms with
Various modifications, addition and replacement in details.Certainly, these are made according to present invention spirit various modifications, addition and replacement
Deng change, all should be included within scope of the present invention.
Claims (3)
1. a kind of frog heart perfusion experiment device, including tremulous pulse plug, arterial cannulation, the quantization drop dress connected with the arterial cannulation
The venous cannulation put, being arranged on below the quantization drip apparatus, the vein plug connected with the venous cannulation, the tremulous pulse is inserted
On the aorta of the head insertion frog heart, on the posterior vena cava of the vein plug insertion frog heart, it is characterised in that arrange including top
The separator tube for having opening, the threeway connected with the separator tube buffer arterial, the arm connection of threeway buffering arterial
Another arm connection arterial cannulation of tremulous pulse plug, threeway buffering arterial.
2. a kind of frog heart perfusion experiment device according to claim 1, it is characterised in that the quantization drip apparatus include note
Dropper, note dropping electrode and note drop guide line.
3. a kind of frog heart perfusion experiment device according to claim 1, it is characterised in that the quantization drip apparatus record side
Formula is that 16 drops are calculated as 1ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510241994.0A CN104825233B (en) | 2015-05-13 | 2015-05-13 | Experimental device for frog heart perfusion |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510241994.0A CN104825233B (en) | 2015-05-13 | 2015-05-13 | Experimental device for frog heart perfusion |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104825233A CN104825233A (en) | 2015-08-12 |
| CN104825233B true CN104825233B (en) | 2017-05-03 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510241994.0A Expired - Fee Related CN104825233B (en) | 2015-05-13 | 2015-05-13 | Experimental device for frog heart perfusion |
Country Status (1)
| Country | Link |
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| CN (1) | CN104825233B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108091231A (en) * | 2017-12-07 | 2018-05-29 | 梁辰 | A kind of frog heart perfusion experiment device and its application process |
| CN108196042A (en) * | 2018-03-16 | 2018-06-22 | 延边大学 | Amphibian Perfused isolated heart device |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4459977A (en) * | 1981-03-27 | 1984-07-17 | Veronique Pizon | Coronary sinus retroperfusion apparatus for the treatment of myocardial ischemia |
| US5687718A (en) * | 1994-07-09 | 1997-11-18 | Hewlett-Packard Company | Device for continuously detecting blood parameters |
| CN2582078Y (en) * | 2002-08-28 | 2003-10-22 | 王庆山 | Apparatus for counting drops of in vitro frog heart perfusion |
| CN201293783Y (en) * | 2008-10-13 | 2009-08-19 | 武汉大学 | Device for circulation and perfusion of in-vitro heart of laboratory animal |
| CN201441461U (en) * | 2009-07-10 | 2010-04-28 | 泰山学院 | Novel frog heart perfusion experiment device |
-
2015
- 2015-05-13 CN CN201510241994.0A patent/CN104825233B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4459977A (en) * | 1981-03-27 | 1984-07-17 | Veronique Pizon | Coronary sinus retroperfusion apparatus for the treatment of myocardial ischemia |
| US5687718A (en) * | 1994-07-09 | 1997-11-18 | Hewlett-Packard Company | Device for continuously detecting blood parameters |
| CN2582078Y (en) * | 2002-08-28 | 2003-10-22 | 王庆山 | Apparatus for counting drops of in vitro frog heart perfusion |
| CN201293783Y (en) * | 2008-10-13 | 2009-08-19 | 武汉大学 | Device for circulation and perfusion of in-vitro heart of laboratory animal |
| CN201441461U (en) * | 2009-07-10 | 2010-04-28 | 泰山学院 | Novel frog heart perfusion experiment device |
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| Publication number | Publication date |
|---|---|
| CN104825233A (en) | 2015-08-12 |
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Granted publication date: 20170503 Termination date: 20180513 |