CN104825233A - Improved experimental device for frog heart perfusion - Google Patents
Improved experimental device for frog heart perfusion Download PDFInfo
- Publication number
- CN104825233A CN104825233A CN201510241994.0A CN201510241994A CN104825233A CN 104825233 A CN104825233 A CN 104825233A CN 201510241994 A CN201510241994 A CN 201510241994A CN 104825233 A CN104825233 A CN 104825233A
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- CN
- China
- Prior art keywords
- frog heart
- artery
- plug
- arterial
- tube
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
Abstract
The invention relates to an experimental device for medical function and physiology, in particular to an improved experimental device for frog heart perfusion. The improved experimental device comprises a buffer tube, a three-way buffer artery tube, an artery plug, an artery cannula, a quantitative drop device, a venous cannula and a venous plug, wherein an opening is formed in the top of the buffer tube; the three-way buffer artery tube is connected with the buffer tube; the artery plug is connected with one branch tube of the three-way buffer artery tube; the artery cannula is connected with the other branch tube of the three-way buffer artery tube; the quantitative drop device is connected with the artery cannula; the venous cannula is arranged below the quantitative drop device; the venous plug is connected with the venous cannula; the artery plug is inserted into an aorta of a frog heart; the venous plug is inserted into a postcaval vein of the frog heart. According to the improved experimental device for the frog heart perfusion, disclosed by the invention, the buffer action for perfused liquid ejected in the blood ejection process of the frog heart is realized, uninterrupted dropwise outflow of the perfused liquid can be kept, the perfused liquid can flow at the systole and diastole of the heart, and quantitative record of liquid can be realized by the quantitative drop device.
Description
Technical field
The present invention relates to Medical Function, Physiology Experiment device, be specifically related to a kind of improvement frog heart perfusion experiment device.
Background technology
Perfused isolated heart is one of important means of research cardiac function, is widely used in the teaching and scientific research work such as medical science, biology, pharmacy.Adopt Perfused isolated heart system can observe the activity of heart intuitively, research heart systolic and diastolic function, heart rate and rhythm and pace of moving things change, the impact on cardiac function such as front and back load and various humoral factors.Adopting frog heart to carry out Perfused isolated heart is a kind of model comparatively commonly used, because it is stablized, economical, is extensively adopted by people always.Carry out Perfused isolated heart experiment, first need to ensure heart long period survival in vitro, then experimentally object is carried out perfusion and is applied empirical factor.Be no matter heart in vitro before the operation such as intubate, blood flow flushing, or heart in vitro after replace blood to carry out perfusion by artificial solution, the physiological neuro humor environment that all made heart depart from.Therefore, survived the isolated heart long period, and it is very important for completing experiment and obtaining reliable experimental data to maintain good state.
Be column injection in paradoxical expansion infusion liquid in traditional experiment device, and relaxing period is because penetrating without infusion liquid without pressure.Primitive apparatus, without separator tube, cannot ensure that relaxing period infusion liquid penetrates, and systole then becomes column to penetrate, and is not easy to the quantification of frog heart liquid jet.
Summary of the invention
An object of the present invention cannot ensure that relaxing period infusion liquid penetrates for solving, be not easy to the quantification problem of frog heart liquid jet, there is provided a kind of and improve frog heart perfusion experiment device, the infusion liquid penetrated in blood process is penetrated to the frog heart and plays cushioning effect, the continual drop-wise of infusion liquid can be kept to flow out, all there is infusion liquid to flow out at paradoxical expansion and relaxing period, liquid can be realized by apparatus for counting drops and quantize record.
In order to reach above-mentioned technique effect, technical scheme of the present invention comprises:
A kind of improvement frog heart perfusion experiment device, comprise the separator tube that top is provided with opening, the threeway buffering arterial connected with described separator tube, the tremulous pulse plug connected with an arm of described threeway buffering arterial, the arterial cannulation connected with another arm of described threeway buffering arterial, the quantification drip apparatus connected with described arterial cannulation, the vein plug that is arranged on venous cannulation below described quantification drip apparatus, connects with described venous cannulation, described tremulous pulse plug inserts on the aorta of the frog heart, and described vein plug inserts on the posterior vena cava of the frog heart.
Described quantification drip apparatus comprises note dropper, note dropping electrode and note and drips guide line.
Described quantification drip apparatus recording mode is 16 and counts 1ml.
Beneficial effect of the present invention comprises:
1, the present invention's one improves frog heart perfusion experiment device, the infusion liquid penetrated in blood process is penetrated to the frog heart and plays cushioning effect, the continual drop-wise of infusion liquid can be kept to flow out, all have infusion liquid to flow out at paradoxical expansion and relaxing period, drip apparatus can realize liquid quantification record by quantifying.
2, the present invention's one improves frog heart perfusion experiment device, to a certain degree can improve the temperature of circulating fluid, keeps the activity of frog heart specimen.
3, the present invention's one improves frog heart perfusion experiment device, and device is simple, easily operates, and measures the physiological datas such as frog cardiac output, aorta blood volume more accurately, improves the accuracy rate of experiment, success rate.
Accompanying drawing explanation
Figure 1 shows that the present invention's one improves frog heart perfusion experiment device schematic diagram.
Figure 2 shows that a kind of A place partial enlarged drawing improving frog heart perfusion experiment device Fig. 1 of the present invention.
Diagram: 1-separator tube, 2-threeway buffering arterial, 3-tremulous pulse plug, 4-arterial cannulation, 5-quantize drip apparatus, 51-remembers that dropper, 52-remember that dropping electrode, 53-note drips guide line, 6-venous cannulation, 7-vein plug, 8-biological functional system.
Detailed description of the invention
Hereafter will describe the specific embodiment of the invention in detail in conjunction with concrete accompanying drawing.It should be noted that the combination of technical characteristic or the technical characteristic described in following embodiment should not be considered to isolated, they can mutually be combined thus be reached better technique effect.In the accompanying drawing of following embodiment, the identical label that each accompanying drawing occurs represents identical feature or parts, can be applicable in different embodiment.
Embodiment:
The present invention's one improves frog heart perfusion experiment device schematic diagram as shown in Figure 1.A kind of improvement frog heart perfusion experiment device, comprise the separator tube 1 that top is provided with opening, the threeway buffering arterial 2 connected with described separator tube 1, with the tremulous pulse plug 3 that an arm of described threeway buffering arterial 2 connects, with the arterial cannulation 4 that another arm of described threeway buffering arterial 2 connects, with the quantification drip apparatus 5 that described arterial cannulation 4 connects, be arranged on the venous cannulation 6 below described quantification drip apparatus 5, with the vein plug 7 that described venous cannulation 6 connects, described tremulous pulse plug 3 inserts on the aorta of the frog heart, described vein plug 7 inserts on the posterior vena cava of the frog heart.
As shown in Figure 2, described quantification drip apparatus 5 comprises note dropper 51, note dropping electrode 52 and a note guide line 53.
Described quantification drip apparatus 5 recording mode is 16 and counts 1ml.
When preparing perfusion experiment, connect experimental provision, destroy frog spinal cord and brain with probe, dorsal position is fixed on frog board.Open thoracic cavity and expose the frog heart, cut off pericardium, first in the separator tube 1 of device, fill ringer solution, the arterial cannulation 3 in handle assembly inserts in frog aorta, fixes ligation with line; The frog heart is upwards turned over, exposes caval vein, the venous cannulation 6 in device is inserted ligation in caval vein and fixes.Cut off blood vessel with operating scissors, the frog heart is proposed in thoracic cavity, is fixed on universal stand.Experimental provision of the present invention is connected with biological functional system 8.There is the quantification drip apparatus 5 that note is dripped in a device.Quantize drip apparatus 5 to be made up of note dropper 51, note dropping electrode 52 and a note guide line 53.Quantize drip apparatus 5 to be placed in directly over venous cannulation 6.Quantize drip apparatus 5 and be connected biological functional system 8 by note dropping electrode 52 and a note guide line 53, biological functional system 8 is connected with computer, when there being drips, responded to by note dropping electrode 52, and induced signal is delivered to biological functional system 8, just can write down on computers, thus frog cardiac output can be measured realize quantification effect.
Separator tube 1, based on buffering and power conversion, adds infusion in separator tube 1.Separator tube 1 is communicated with threeway buffering arterial 2, an arm of described threeway buffering arterial 2 connects tremulous pulse plug 3, another arm of described threeway buffering arterial 2 connects arterial cannulation 4, after tremulous pulse plug 3 inserts aorta, threeway buffering arterial 2 is through distributing T-pipe, an infusion liquid part is made to enter tremulous pulse plug 3, another part enters through arterial cannulation 4 and quantizes drip apparatus 5, quantification record is carried out through quantizing drip apparatus 5, remembering 16 is 1ml, and then infusion liquid arrives vein plug 7 times hearts through venous cannulation 6.Formed an extracorporeal circulation at body frog heart perfusion device.
The present invention's one improves frog heart perfusion experiment device, the infusion liquid penetrated in blood process is penetrated to the frog heart and plays cushioning effect, the continual drop-wise of infusion liquid can be kept to flow out, all have infusion liquid to flow out at paradoxical expansion and relaxing period, drip apparatus can realize liquid quantification record by quantifying.
Above-mentioned detailed description is the illustrating of possible embodiments for invention, and this embodiment is also not used to limit the scope of the claims of the present invention, does not allly depart from equivalence of the present invention and implements or change, and all should be contained in the scope of the claims of the present invention.
In addition, those skilled in the art also can make various amendments in other form and details, interpolation and replacement in the claims in the present invention scope of disclosure and spirit.Certainly, the changes such as these various amendments made according to the present invention's spirit, interpolation and replacement, all should be included within the present invention's scope required for protection.
Claims (3)
1. one kind is improved frog heart perfusion experiment device, it is characterized in that, comprise the separator tube that top is provided with opening, the threeway buffering arterial connected with described separator tube, with the tremulous pulse plug that an arm of described threeway buffering arterial connects, with the arterial cannulation that another arm of described threeway buffering arterial connects, the quantification drip apparatus connected with described arterial cannulation, be arranged on the venous cannulation below described quantification drip apparatus, the vein plug connected with described venous cannulation, described tremulous pulse plug inserts on the aorta of the frog heart, described vein plug inserts on the posterior vena cava of the frog heart.
2. one according to claim 1 improves frog heart perfusion experiment device, it is characterized in that, described quantification drip apparatus comprises note dropper, note dropping electrode and note and drips guide line.
3. one according to claim 1 improves frog heart perfusion experiment device, and it is characterized in that, described quantification drip apparatus recording mode is 16 and counts 1ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510241994.0A CN104825233B (en) | 2015-05-13 | 2015-05-13 | Experimental device for frog heart perfusion |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510241994.0A CN104825233B (en) | 2015-05-13 | 2015-05-13 | Experimental device for frog heart perfusion |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104825233A true CN104825233A (en) | 2015-08-12 |
| CN104825233B CN104825233B (en) | 2017-05-03 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510241994.0A Expired - Fee Related CN104825233B (en) | 2015-05-13 | 2015-05-13 | Experimental device for frog heart perfusion |
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| Country | Link |
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| CN (1) | CN104825233B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108091231A (en) * | 2017-12-07 | 2018-05-29 | 梁辰 | A kind of frog heart perfusion experiment device and its application process |
| CN108196042A (en) * | 2018-03-16 | 2018-06-22 | 延边大学 | Amphibian Perfused isolated heart device |
| CN110320351A (en) * | 2019-08-21 | 2019-10-11 | 延边大学 | Isolated frog heart perfusion experiment instructional device |
| RU2815290C1 (en) * | 2023-05-26 | 2024-03-13 | Акционерное Общество КардиоСистемФарма | Perfusion method and perfusion system device for donor heart preservation |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4459977A (en) * | 1981-03-27 | 1984-07-17 | Veronique Pizon | Coronary sinus retroperfusion apparatus for the treatment of myocardial ischemia |
| US5687718A (en) * | 1994-07-09 | 1997-11-18 | Hewlett-Packard Company | Device for continuously detecting blood parameters |
| CN2582078Y (en) * | 2002-08-28 | 2003-10-22 | 王庆山 | Apparatus for counting drops of in vitro frog heart perfusion |
| CN201293783Y (en) * | 2008-10-13 | 2009-08-19 | 武汉大学 | Device for circulation and perfusion of in-vitro heart of laboratory animal |
| CN201441461U (en) * | 2009-07-10 | 2010-04-28 | 泰山学院 | Novel frog heart perfusion experiment device |
-
2015
- 2015-05-13 CN CN201510241994.0A patent/CN104825233B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4459977A (en) * | 1981-03-27 | 1984-07-17 | Veronique Pizon | Coronary sinus retroperfusion apparatus for the treatment of myocardial ischemia |
| US5687718A (en) * | 1994-07-09 | 1997-11-18 | Hewlett-Packard Company | Device for continuously detecting blood parameters |
| CN2582078Y (en) * | 2002-08-28 | 2003-10-22 | 王庆山 | Apparatus for counting drops of in vitro frog heart perfusion |
| CN201293783Y (en) * | 2008-10-13 | 2009-08-19 | 武汉大学 | Device for circulation and perfusion of in-vitro heart of laboratory animal |
| CN201441461U (en) * | 2009-07-10 | 2010-04-28 | 泰山学院 | Novel frog heart perfusion experiment device |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108091231A (en) * | 2017-12-07 | 2018-05-29 | 梁辰 | A kind of frog heart perfusion experiment device and its application process |
| CN108196042A (en) * | 2018-03-16 | 2018-06-22 | 延边大学 | Amphibian Perfused isolated heart device |
| CN110320351A (en) * | 2019-08-21 | 2019-10-11 | 延边大学 | Isolated frog heart perfusion experiment instructional device |
| RU2815290C1 (en) * | 2023-05-26 | 2024-03-13 | Акционерное Общество КардиоСистемФарма | Perfusion method and perfusion system device for donor heart preservation |
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| Publication number | Publication date |
|---|---|
| CN104825233B (en) | 2017-05-03 |
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Granted publication date: 20170503 Termination date: 20180513 |