CN104807792B - A kind of negative index photonic crystal type bioluminescence detector - Google Patents
A kind of negative index photonic crystal type bioluminescence detector Download PDFInfo
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- CN104807792B CN104807792B CN201510203559.9A CN201510203559A CN104807792B CN 104807792 B CN104807792 B CN 104807792B CN 201510203559 A CN201510203559 A CN 201510203559A CN 104807792 B CN104807792 B CN 104807792B
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- photonic crystal
- negative index
- index photonic
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Abstract
The invention discloses a kind of negative index photonic crystal type bioluminescence detector, including main body box, optical substrate and transparent colloid, negative index photonic crystal in main body box is alternateed and overlapped to form by piezoelectric material layer and material with negative refractive index layer, piezoelectric material layer and control power electric connection, change negative index photonic crystal narrowband transfer characteristic, the negative index photonic crystal is attached in optical substrate, the upper surface of negative index photonic crystal sets the first photodetector and the second photodetector, first photodetector and the second photodetector are received and excite fluorescence signal through negative index photonic crystal is filtered, and excite fluorescence signal to be converted into electric signal this, electric signal sends processor to and is processed.The single device of the present invention is the filtering of the selection and wavelength of fluorescence for being capable of achieving excitation laser wavelength, and various fluorescence tissues can be detected simultaneously, solve the quantitative computational problem that different wave length causes, and fluorescence measurement precision reaches 96.8%.
Description
Technical field
The present invention relates to biological tissue's Autofluorescence analysis technical field, and in particular to a kind of negative index photon is brilliant
Build bioluminescence detector.
Background technology
Fluorescence emission spectrum is that fluorescent material absorbs the result launched again after radiation, when fluorescence excitation spectrum refers to different ripples
Long to excite the relative efficiency for causing and launching a certain wavelength fluorescent, fluorescence intensity is with the fluorescent material fluorescence emission spectrum
The integral area amount strong and weak to represent fluorescence.Recently as the appearance of different fluorescent markers, intracellular different cells are marked
Device or the intracellular different ions of detection are possibly realized, and the fluorescence emitting characteristics according to fluorescent marker select suitable exciting light
Source, living body fluorescent imaging technique within several years for just having grown up, be widely used to Cellular tracking, diagnosing tumor and
The aspects such as drug development, show huge application potential in clinical practice.
In current biological tissue XRF with its sensitivity it is high, selectivity is good, easy to operate the advantages of analyzed
The favor of worker, Pharmaceutical Analysis is applied to by XRF, is analyzed and identified in effective ingredient, pharmacokinetic
Learn the aspects such as research, clinical medicine and Toxicity Analysis and obtain tremendous development, and be widely used in the neck such as biochemical analysis, biomedicine
The trace analysis in domain.
China has been disclosed for multinomial patent, such as《A kind of microfluidic system of single beam biological cell detection and screening and
Method》(CN103439242 A) is marked using up-conversion luminescence nanomaterial to problem cell in target to be measured, using near
The method of infrared band laser induction up-conversion luminescence carries out the detection of problem cell by laser scanning optical detection means.
《A kind of method based on gold nano cluster fluorescence probe quick detection concentration of glucose》(CN103837516 A) and《For visiting
The apparatus and method for surveying the biological components that fluorogen marked》(CN101410708A) a kind of simple fluid sample is then provided
In the fluorogen mark biological components method that carries out technical Analysis.Foregoing invention patent in order to obtain corresponding fluorescent characteristic,
Using the excitation source that incident wavelength is single, this excitation source cannot be adjusted, unitary function, it is impossible to while recognizing multiple glimmering
Light tissue.
The content of the invention
For above-mentioned prior art, visited it is an object of the invention to provide a kind of negative index photonic crystal type bioluminescence
Device is surveyed, it is intended to be capable of achieving the selection of excitation laser wavelength using single device and adjusts biological tissue's fluorescence excitation ripple
It is long, so as to reach the purpose that various fluorescence tissues are detected simultaneously, and it is prevented effectively from the interference of the fluorescence tissue of other wavelength.
In order to solve the above-mentioned technical problem, the present invention is adopted the following technical scheme that：
A kind of negative index photonic crystal type bioluminescence detector, including optical substrate 12 and transparent colloid 13, optics
Substrate be located at transparent colloid on, it is characterised in that also including main body box 11, the main body box 11 be placed in optical substrate 12 it
On, main body box is mainly by laser 14, collimation lens 15, display module 16, laser driving circuit 18, processor, negative refraction
Rate photon crystal 1 10, the first photodetector 111, the second photodetector 113 and control power supply 115 are constituted；Processor is connected
Control laser driving circuit 18, laser driving circuit 18 drives the underface of laser, laser 14 to install the collimation
Lens 15；The negative index photon crystal 1 10 is alternateed and overlapped to form by piezoelectric material layer and material with negative refractive index layer, is pressed
Material layer and control power electric connection, change negative index photonic crystal arrowband, and the negative index photon crystal 1 10 adheres to
In optical substrate, the upper surface of negative index photon crystal 1 10 sets the smooth electrical resistivity survey of first photodetector 111 and second
Device 113 is surveyed, the first photodetector 111 and the second photodetector 113 are located at the lower section both sides of collimation lens 15；First smooth electrical resistivity survey
The survey photodetector 113 of device 111 and second is received and excites fluorescence signal through negative index photon crystal 1 10 is filtered, and will
This excites fluorescence signal to be converted into electric signal, and electric signal sends processor to and processed, and result is displayed in display module
On 16.
In the present invention, negative index photonic crystal type bioluminescence detector, also including transparent colloid, on transparent colloid
The optical substrate is set, and the main body box 11 is located at the surface of optical substrate.
In the present invention, the processor is dsp processor, arm processor or single-chip microcomputer.
In the present invention, the main body box 11 also includes light-absorbing coating 112, and the light-absorbing coating is attached to optical substrate 12
On, the unnecessary scattering fluorescence that absorption is transmitted from skin reduces environmental disturbances signal.
In the present invention, wavelength of fluorescence adjustment button 17 is additionally provided with the main body box, the wavelength of fluorescence adjustment is pressed
Button is connected with P processors 19, and control laser launches different specific excitation wavelengths.
In the present invention, the laser 14 is short pulse tunable laser, and wave-length coverage is 150nm~460nm,
Further, the short pulse tunable laser is the ultraviolet or nearly purple light that wavelength is 230nm~345nm
Pulse laser.
In the present invention, the negative index photon crystal 1 10 is made up of one-dimensional negative index photonic crystal, while tool
There are arrowband and wideband filtered characteristic.
In the present invention, the transparent colloid is made up of flexible PVC colloidal sol, with isotropic feature, thickness
Less than 0.2mm.
In the present invention, the photodetector 113 of first photodetector 111 and second is photodiode or photoelectricity
Multiplier tube, detection range is 860~1500nm, and optimum detection scope is 930~1150nm.
Operation principle of the invention is：
After exciting light incides collimation lens, incide negative index photonic crystal, due to negative index photonic crystal by
What material with negative refractive index and piezoelectric were constituted, while having arrowband and wideband filtered characteristic, piezoelectric therein is received
To control power supply so that the arrowband of negative index photonic crystal is moved；If the wavelength of exciting light is in negative index
In the range of photonic crystal arrowband, then exciting light enters by after negative index photonic crystal, optical substrate and transparent colloid
Skin, so as to form fluorescence with corresponding biological tissue, the fluorescence for exciting is transmitted from skin, passes sequentially through optical substrate
Become electric signal with being received by a photoelectric detector after negative index photonic crystal, this electric signal is sent in processor after carrying out to be located
Reason；When the wavelength of exciting light is not fallen within the arrowband of negative index photonic crystal, then exciting light is complete by negative index photonic crystal
Reflection, it is impossible to enter into skin, so as to the signal for avoiding other excitation wavelengths is disturbed.
Compared with prior art, the invention has the advantages that：
First, excitation source incident wavelength is controllable.The launch wavelength of laser is adjusted by laser drive circuit, while controlling electricity
Source adjusts the refractive index of piezoelectric, so as to directly affect the propagation characteristic of negative index photonic crystal, reaches adjustment adjustment and swashs
Luminous narrow-band filtering characteristic matches fluorescence spectrum, solves the quantitative computational problem that different wave length causes, and improves fluorescence measurement
Precision reaches 96.8%.
2nd, it is the filtering of the selection and wavelength of fluorescence for being capable of achieving excitation laser wavelength using single device, by fluorescence ripple
Adjustment button control laser long launches different specific excitation wavelength and the arrowband filters using negative index photonic crystal
Wave property can be realized being detected while various fluorescence tissues, using simple, realize conveniently.
3rd, light-absorbing coating is provided with optical substrate, exciting light selection narrow-band filtering, light-absorbing coating absorption is saturating from skin
The unnecessary scattering fluorescence for shooting out, it is possible to prevente effectively from the influence of other fluorescence tissues.
Brief description of the drawings
Fig. 1 is the structural representation of the embodiment of the present invention one；
Fig. 2 is the periodic structure schematic diagram of negative index photonic crystal in the embodiment of the present invention one；
Fig. 3 is the transmission feature figure of negative index photonic crystal in the embodiment of the present invention one；
Fig. 4 is negative index photonic crystal transmission feature figure under different modulating in the embodiment of the present invention one.
The fluorescent energy figure that Fig. 5 goes out for different wave length laser excitation in the embodiment of the present invention one.
Reference is：11 is main body box, 12 is optical substrate, 13 is transparent colloid, 14 is laser, 15 is to collimate thoroughly
Mirror, 16 be display module, 17 be wavelength of fluorescence adjustment button, 18 be laser driving circuit, 19 be dsp processor, 110 be negative
Refractive index photonic crystal, 111 be the first photodetector, 112 be light-absorbing coating, 113 be the second photodetector, 114 be interior
Power supply, 115 are put to control power supply.
Specific embodiment
Below in conjunction with the drawings and the specific embodiments, the invention will be further described.
A kind of negative index photonic crystal type bioluminescence detector, including optical substrate 12 and transparent colloid 13, optics
Substrate be located at transparent colloid on, it is characterised in that also including main body box 11, the main body box 11 be placed in optical substrate 12 it
On, main body box is mainly by laser 14, collimation lens 15, display module 16, laser driving circuit 18, dsp processor 19, negative
Refractive index photon crystal 1 10, the first photodetector 111, the second photodetector 113 and control power supply 115 are constituted；At DSP
The reason connection control laser driving circuit 18 of device 19, laser driving circuit 18 drives laser 14, the underface of laser 14
The collimation lens 15 is installed；The negative index photonic crystal alternates weight by piezoelectric material layer and material with negative refractive index layer
Folded formation, piezoelectric material layer and control power electric connection, change negative index photonic crystal arrowband, and the negative index photon is brilliant
Body 110 is attached in optical substrate, and the upper surface of negative index photon crystal 1 10 sets the He of the first photodetector 111
Second photodetector 113, the first photodetector 111 and the second photodetector 113 are located at the lower section both sides of collimation lens 15；
First photodetector 111 and the second photodetector 113 are received and excite fluorescence through negative index photon crystal 1 10 is filtered
Signal, and excite fluorescence signal to be converted into electric signal this, electric signal sends dsp processor 19 to and is processed, result
It is displayed on display module 16.The present invention uses built-in power 114, with good portability.
Formation mechenism of the invention：Negative index photon crystal 1 10 is made up of material with negative refractive index and piezoelectric, and
And there is arrowband and wideband filtered characteristic simultaneously, piezoelectric therein is controlled power supply 115 and controls so that negative index light
The arrowband of sub- crystal 110 is moved；If the wavelength of exciting light is in the range of the arrowband of negative index photon crystal 1 10,
Exciting light by entering skin after negative index photon crystal 1 10, optical substrate 12 and transparent colloid 13, so as to it is corresponding
Biological tissue forms fluorescence, and the fluorescence for exciting is transmitted from skin, passes sequentially through optical substrate 12 and negative index photon
Received by the first photodetector and the second photodetector 113 after crystal 110, the signal of reception is sent in dsp processor 19,
Unnecessary scattering fluorescence is absorbed by light-absorbing coating material 112, reduces environmental disturbances signal.
Embodiment one：
A kind of negative index photonic crystal type bioluminescence panel detector structure is as shown in Figure 1 in this example.Wherein negative index
Photonic crystal be by refractive index be respectively -0.65 material with negative refractive index and refractive index for 2.297 piezoelectrics are alternately present group
Into, wherein jth layer of piezo-electric material is controlled power supply 115 and controls, as shown in Figure 2.
The transmission feature figure of the negative index photonic crystal as shown in figure 3, it has arrowband and wideband filtered characteristic simultaneously,
Wherein in short wavelength region, (wave-length coverage is 324nm~326nm) has very sharp filtering bandwidth and long wavelength region (wavelength model
It is 864~1088nm to enclose) there is a filtering bandwidth wider, light can only could be by negative index photonic crystal in this two sections of wavelength
110。
Can become the piezoelectric thickness being made up of dielectric elastomeric material by controlling the applied voltage of power supply 115
Change, empirical equation is represented by：
Wherein elastic strain amplitude S is relevant with applied voltage V, and d is the thickness of piezoelectric, and P is the bullet light of piezoelectric
Coefficient, Δ n is modulation depth.The negative index in the case of modulation depth is respectively Δ n=0, Δ n=0.1, tri- kinds of Δ n=0.2
The change of photonic crystal arrowband and wideband filtered characteristic, as shown in Figure 4.
While it is relevant with excitation wavelength using the fluorescence that different short wavelength lasers are inspired, as long as so excitation light is more than certain
Individual value can be encouraged, but there is quantitative problem, and the energy of the luminescence generated by light obtained by different excitation wavelengths is different, such as schemes
Shown in 5.
When measuring, button 17 can be adjusted by wavelength of fluorescence and selects different specific excitation wavelengths, dsp processor 19
After receiving selected wavelength, the launch wavelength of laser 14 is controlled by laser drive circuit 18, simultaneous processor 19 is sent out
Predeterminated voltage signal is sent to control piezoelectric by voltage to control power supply 115, control power supply 115 so that negative index photon
The arrowband of crystal 110 is moved, so that narrow-band filtering characteristic can just meet the wavelength of laser 14, realizes that arrowband is filtered
Ripple, then exciting light is by entering skin after negative index photon crystal 1 10, optical substrate 12 and transparent colloid 13, so as to
Corresponding biological tissue forms fluorescence, and the fluorescence for exciting is transmitted from skin, passes sequentially through optical substrate 12 and negative refraction
Received by the first photodetector and the second photodetector after rate photon crystal 1 10, the signal of reception is sent to dsp processor 19
In post-processed, due to related operation can be used using two detectors, effectively improve treatment effeciency and the degree of accuracy, finally
The result for the treatment of is sent to display module 16.The trouble for exchanging LASER Light Source so can be both eliminated, can be quantified again and be examined
Survey, while avoiding the interference from other fluorescence, improve fluorescence measurement precision and reach 96.8%.
Because current Photonic Band computational methods are ripe, it is sufficient to ensure to pass through the reasonably combined formation structure of material
The forbidden band scope of middle needs, while by introducing form paracycle or other structures in the structure, it is also possible to so that photonic crystal
The Properties Forbidden Band of needs is produced in forbidden band scope.
It should be understood that the above embodiments are merely to illustrate technical scheme rather than its limitations.Art
Technical staff by design different photonic crystal band features can construct easily different from above-described embodiment one one
Plant negative index photonic crystal type bioluminescence detector.Therefore, the present invention should include all that in appended claims
Changes, modifications and variations in the range of book.
Claims (10)
1. a kind of negative index photonic crystal type bioluminescence detector, it is characterised in that including optical substrate (12), main body box
(11), the main body box (11) is placed on optical substrate (12), main body box mainly by laser (14), collimation lens (15),
Display module (16), laser driving circuit (18), processor (19), negative index photonic crystal (110), the first photodetection
Device (111), the second photodetector (113) and control power supply (115) are constituted；Processor (19) connection control laser drives electricity
Road (18), laser driving circuit (18) drives the underface of laser, laser (14) to install the collimation lens (15)；Institute
State negative index photonic crystal (110) to be alternateed and overlapped to form by piezoelectric material layer and material with negative refractive index layer, piezoelectric
Layer and control power electric connection, change negative index photonic crystal arrowband, and the negative index photonic crystal (110) is attached to light
Learn in substrate (12), the upper surface of negative index photonic crystal (110) sets first photodetector (111) and the second light
Electric explorer (113), the first photodetector (111) and the second photodetector (113) are positioned at collimation lens (15) lower section two
Side；First photodetector (111) and the second photodetector (113) are received after being filtered through negative index photonic crystal (110)
Excite fluorescence signal, and excite fluorescence signal to be converted into electric signal this, electric signal sends processor to and is processed, treatment
Result is displayed on display module (16).
2. negative index photonic crystal type bioluminescence detector according to claim 1, it is characterised in that also including saturating
Gelatin body (13), sets the optical substrate (12) on transparent colloid, the main body box (11) is positioned at the surface of optical substrate.
3. negative index photonic crystal type bioluminescence detector according to claim 1, it is characterised in that the treatment
Device is dsp processor or arm processor or single-chip microcomputer.
4. negative index photonic crystal type bioluminescence detector according to claim 1, it is characterised in that the main body
Box (11) also includes light-absorbing coating (112), and the light-absorbing coating is attached in optical substrate (12).
5. negative index photonic crystal type bioluminescence detector according to claim 1 and 2, it is characterised in that described
Wavelength of fluorescence is additionally provided with main body box and adjusts button (17), the wavelength of fluorescence adjustment button is connected with processor.
6. negative index photonic crystal type bioluminescence detector according to claim 1, it is characterised in that the laser
Device (14) is short pulse tunable laser, and wave-length coverage is 150nm~460nm.
7. negative index photonic crystal type bioluminescence detector according to claim 6, it is characterised in that the short arteries and veins
It is the ultraviolet or nearly purple light pulse laser that wavelength is 230nm~345nm to rush tunable laser.
8. negative index photonic crystal type bioluminescence detector according to claim 1, it is characterised in that the negative folding
Rate photonic crystal (110) is penetrated to be made up of one-dimensional negative index photonic crystal.
9. negative index photonic crystal type bioluminescence detector according to claim 2, it is characterised in that described transparent
Colloid (13) is made up of flexible PVC colloidal sol, and thickness is less than 0.2mm.
10. negative index photonic crystal type bioluminescence detector according to claim 1, it is characterised in that described
One photodetector (111) and the second photodetector (113) are photodiode or photomultiplier, and detection range is 860
~1500nm.
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Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8304746B2 (en) * | 2007-03-19 | 2012-11-06 | Shimadzu Corporation | Fluorescent measurement device for living body and exciting light-irradiating device for fluorescent measurement |
-
2015
- 2015-04-27 CN CN201510203559.9A patent/CN104807792B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3916197A (en) * | 1973-11-28 | 1975-10-28 | Particle Technology Inc | Method and apparatus for classifying biological cells |
| CN2496015Y (en) * | 2001-04-23 | 2002-06-19 | 吴志音 | Double-mark determining apparatus for laser inducing fluorescence |
| CN101975769A (en) * | 2010-09-17 | 2011-02-16 | 福建师范大学 | Human tissue autofluorescence detection system based on excitation of light sources with different wavelength |
| CN103424387A (en) * | 2013-06-20 | 2013-12-04 | 中国科学院苏州生物医学工程技术研究所 | Fluorescence spectrum detection device for pesticide residue |
Non-Patent Citations (2)
| Title |
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| Fundamentals and practice for ultrasensitive laser-induced fluorescence detection in microanalytical systems;Mitchell E.Johnson,et al.;《Electrophoresis》;20041231;第25卷;第3513-3527页 * |
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