CN104713752A - Smoke collecting device and smoke collecting method for evaluating biological effects of electronic cigarette smoke - Google Patents
Smoke collecting device and smoke collecting method for evaluating biological effects of electronic cigarette smoke Download PDFInfo
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- CN104713752A CN104713752A CN201510147058.3A CN201510147058A CN104713752A CN 104713752 A CN104713752 A CN 104713752A CN 201510147058 A CN201510147058 A CN 201510147058A CN 104713752 A CN104713752 A CN 104713752A
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- flue gas
- smoke
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- electronic cigarette
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Abstract
The invention discloses a smoke collecting device and a smoke collecting method for evaluating biological effects of electronic cigarette smoke. The device is formed by combining a smoke collecting bottle, and a smoke collecting liquor transfer tube, wherein the smoke collecting liquor transfer tube is inserted into the smoke collecting bottle; a smoke outlet and a smoke inlet are formed in the smoke collecting bottle; and the position of the smoke inlet is higher than the smoke outlet. The smoke collecting liquor transfer tube is used for getting a proper amount of collecting liquor from a biological safety cabinet and adding the collected collecting liquor into the smoke collecting bottle, and a smoke inlet path rubber tube of a linear smoke sucker is tightly connected to the smoke inlet; and an automatic smoke sucker procedure is set to perform smoke collecting. The smoke collecting device is simple in structure and convenient to operate, the collecting liquor is not prone to pollution, main ingredients of the electronic cigarette smoke can be directly and effectively collected, and technical support is provided for effectively evaluating the biological effects of the electronic cigarette smoke.
Description
Technical field
The present invention relates to a kind of smoke gathering device for the evaluation of electronic cigarette flue gas biological effect and method.
Background technology
Electronic cigarette, has another name called nicotine electron delivery system, is a kind ofly to consume product containing nicotine steam via suction nozzle, and by atomizer, electronic cigarette tobacco tar is atomized into flue gas, directly sucks, whole process does not experience burning.The composition of electronic cigarette tobacco tar comprises nicotine, propylene glycol, glycerine, odor additive and water etc., is the electronic atomized liquid coordinating electronic cigarette to use.Namely electronic cigarette flue gas is the flue gas as cigarette produced by electronic smoke atomizer Heating Electron cigarette tobacco tar.Current research shows, the principal ingredient of electronic cigarette flue gas has some compositions soluble in water such as glycerine, nicotine, acetaldehyde, propylene glycol monomethyl ether, phenmethylol and benzoic acid.
Because the principle that electronic cigarette flue gas produces and composition all have very big difference with cigarette, the biological effect therefore produced electronic cigarette flue gas is evaluated and is extremely necessary.When biological effect evaluation is carried out to traditional cigarette flue gas, the mode of main employing cambridge filter trapping main flume, but owing to containing a large amount of glycerine in flue gas when evaluating the biological effect of electronic cigarette flue gas, cambridge filter overload is easily there is when puff number is too much, follow-uply also to extract with DMSO, therefore be necessary to set up a kind of method of simple, rapid acquiring electronic cigarette flue gas, provide technical support for electronic cigarette flue gas carries out biological effect evaluation accurately and efficiently.For traditional cigarette, also useful trapping solution device that traditional cigarette flue gas is trapped, as " a kind of device 201120063251.6 for the full fume exposure of cigarette ", but when carrying out electronic cigarette flue gas trapping with this device, trapping solution easily enters smoking machine from exhanst gas outlet suck-back, and trapping solution is directly poured out from the bottleneck of full fume exposure bottle, easily pollutes trapping solution, therefore, this exposing device is not suitable for the trapping of electronic cigarette flue gas.
Summary of the invention
The object of the present invention is to provide a kind of smoke gathering device for the evaluation of electronic cigarette flue gas biological effect and method, provide technical support for electronic cigarette flue gas carries out biological effect evaluation accurately and efficiently.
The smoke gathering device of electronic cigarette flue gas biological effect of the present invention evaluation is bonded by flue gas trapping bottle, flue gas trapping liquid transfer pipeline, flue gas trapping liquid transfer pipeline inserts in flue gas trapping bottle, flue gas trapping bottle has flue gas gas outlet and smoke air inlet, the position of smoke air inlet is higher than the position of flue gas gas outlet.
Flue gas trapping liquid transfer pipeline top is ear washing bulb.
Based on this device, the flue gas trapping method for the evaluation of electronic cigarette flue gas biological effect of the present invention is carried out according to the following steps:
The first step: the smoke gathering device of electronic cigarette flue gas biological effect of the present invention evaluation is carried out autoclaving, and dries for subsequent use in an oven;
Second step: get appropriate trapping solution (cell culture medium or bacteria culture media or water etc.) with flue gas trapping liquid transfer pipeline and add in flue gas trapping bottle, the liquid level of trapping solution higher than the root of gas approach, lower than the root of flue gas gas outlet; Flue gas trapping liquid transfer pipeline makes the bottleneck of trapping bottle and the ear washing bulb close contact of flue gas trapping liquid transfer pipeline, ensures the leakproofness in whole trapping chamber after inserting flue gas trapping bottle;
3rd step: linear pattern smoking machine flue gas incoming road sebific duct is closely connected on smoke air inlet;
4th step: one end sebific duct two ends being connected with the filtrator of sebific duct is closely attached to flue gas gas outlet place, then by the sebific duct of the filtrator other end closely and smoking machine loop compact siro spinning technology;
5th step: setting automatic smoking machine program, starts flue gas trapping;
6th step: flue gas trapping terminates, removes the connection rubber tube of this device and smoking machine with the backward of erection sequence;
7th step: subsequent biological test is carried out in the flue gas trapping liquid taking-up having trapped flue gas in trapping bottle with flue gas trapping liquid transfer pipeline.
Compared to the capturing device of the traditional flue gas of trapping, trapping solution liquid level of the present invention, higher than gas approach, lower than exhanst gas outlet, solves the problem that trapping solution suck-back enters smoking machine; Meanwhile, the present invention utilizes ear washing bulb by the trapping solution sucking-off in flue gas trapping bottle, easy, pollute fast, not easily.The pollution problem of suck-back problem and trapping solution can be solved by the invention of practical operation proved, and by follow-up Biological Detection means, can directly, the principal ingredient of effective trap electrons cigarette flue gas, reflect the biological effect of electronic cigarette flue gas.Technical support is provided for electronic cigarette flue gas carries out effective biological effect evaluation.
Accompanying drawing explanation
Fig. 1 is smoke gathering device structural representation of the present invention.
In figure: 1-flue gas trapping liquid transfer pipeline, 2-flue gas gas outlet, 3-smoke air inlet, 4-flue gas trapping bottle, 5-ear washing bulb.
Embodiment
As shown in Figure 1, the smoke gathering device of electronic cigarette flue gas biological effect of the present invention evaluation is bonded by flue gas trapping bottle 4, flue gas trapping liquid transfer pipeline 1, flue gas trapping liquid transfer pipeline 1 inserts in flue gas trapping bottle 4, flue gas trapping bottle has flue gas gas outlet 2 and smoke air inlet 3, the position of smoke air inlet 3 is higher than the position of flue gas gas outlet 2.Flue gas trapping liquid transfer pipeline 1 top is ear washing bulb 5(is the conventional apparatus in laboratory).
This device forms with glass tube blowing by testing by traditional handicraft, and flue gas trapping liquid transfer pipeline is ear washing bulb.This smoke gathering device uses with the connection of orthoscopic smoking machine.
The flue gas trapping method that electronic cigarette flue gas biological effect of the present invention is evaluated is as follows:
The first step: the smoke gathering device of electronic cigarette flue gas biological effect of the present invention evaluation is carried out autoclaving, and dries for subsequent use in an oven;
Second step: get appropriate trapping solution (cell culture medium or bacteria culture media or water etc.) with flue gas trapping liquid transfer pipeline 1 from Biohazard Safety Equipment and add in flue gas trapping bottle 4, the liquid level of trapping solution higher than the root of gas approach 3, lower than the root of flue gas gas outlet 2; Flue gas trapping liquid transfer pipeline makes the bottleneck of trapping bottle and ear washing bulb 5 close contact of flue gas trapping liquid transfer pipeline, ensures the leakproofness in whole trapping chamber after inserting flue gas trapping bottle;
3rd step: linear pattern smoking machine flue gas incoming road sebific duct is closely connected on smoke air inlet 3;
4th step: one end sebific duct two ends being connected with the filtrator of sebific duct is closely attached to flue gas gas outlet 2 place, the sebific duct of the filtrator other end is tight and smoking machine loop compact siro spinning technology again, filter is to prevent the trapping solution in flue gas trapping bottle 4 from entering in smoking machine loop; Described filtrator is existing apparatus.
5th step: setting automatic smoking machine program, starts flue gas trapping;
6th step: flue gas trapping terminates, removes the connection rubber tube of this device and smoking machine with the backward of erection sequence;
7th step: subsequent biological test is carried out in the flue gas trapping liquid taking-up having trapped flue gas in trapping bottle with flue gas trapping liquid transfer pipeline.
Practical application shows, this device reaches designing requirement completely.
Claims (2)
1. the smoke gathering device of an electronic cigarette flue gas biological effect evaluation, it is characterized in that being bonded by flue gas trapping bottle, flue gas trapping liquid transfer pipeline, flue gas trapping liquid transfer pipeline inserts in flue gas trapping bottle, flue gas trapping bottle has flue gas gas outlet and smoke air inlet, the position of smoke air inlet is higher than the position of flue gas gas outlet.
2. a flue gas trapping method for electronic cigarette flue gas biological effect evaluation, is characterized in that utilizing the device described in claim 1 to carry out according to the following steps:
The first step: the smoke gathering device of electronic cigarette flue gas biological effect of the present invention evaluation is carried out autoclaving, and dries for subsequent use in an oven;
Second step: get appropriate trapping solution with flue gas trapping liquid transfer pipeline from Biohazard Safety Equipment and add in flue gas trapping bottle, the liquid level of trapping solution higher than the root of gas approach, lower than the root of flue gas gas outlet; Flue gas trapping liquid transfer pipeline makes the bottleneck of trapping bottle and the ear washing bulb close contact of flue gas trapping liquid transfer pipeline, ensures the leakproofness in whole trapping chamber after inserting flue gas trapping bottle;
3rd step: linear pattern smoking machine flue gas incoming road sebific duct is closely connected on smoke air inlet;
4th step: one end sebific duct two ends being connected with the filtrator of sebific duct is closely attached to flue gas gas outlet place, then by the sebific duct of the filtrator other end closely and smoking machine loop compact siro spinning technology;
5th step: setting automatic smoking machine program, starts flue gas trapping;
6th step: flue gas trapping terminates, removes the connection rubber tube of this device and smoking machine with the backward of erection sequence;
7th step: subsequent biological test is carried out in the flue gas trapping liquid taking-up having trapped flue gas in trapping bottle with flue gas trapping liquid transfer pipeline.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510147058.3A CN104713752B (en) | 2015-03-31 | 2015-03-31 | A kind of smoke gathering device evaluated for electronic cigarette flue gas biological effect and method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510147058.3A CN104713752B (en) | 2015-03-31 | 2015-03-31 | A kind of smoke gathering device evaluated for electronic cigarette flue gas biological effect and method |
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| CN104713752A true CN104713752A (en) | 2015-06-17 |
| CN104713752B CN104713752B (en) | 2017-09-22 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106520910A (en) * | 2016-11-25 | 2017-03-22 | 云南中烟工业有限责任公司 | Method for detecting influences of electronic cigarette products on cell superoxide dismutase activity |
| CN108226136A (en) * | 2018-01-26 | 2018-06-29 | 福州大学 | A kind of device and its application method of quick detection Nicotine in Tobacco content |
| CN109609592A (en) * | 2018-11-28 | 2019-04-12 | 云南中烟工业有限责任公司 | A method of detection heating does not burn Cigarette grain phase to the influence of cellular superoxide hydrogenase activity |
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| US5763360A (en) * | 1994-02-02 | 1998-06-09 | The Regents Of The University Of California | Quantitative organic vapor-particle sampler |
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| CN106520910A (en) * | 2016-11-25 | 2017-03-22 | 云南中烟工业有限责任公司 | Method for detecting influences of electronic cigarette products on cell superoxide dismutase activity |
| CN108226136A (en) * | 2018-01-26 | 2018-06-29 | 福州大学 | A kind of device and its application method of quick detection Nicotine in Tobacco content |
| CN109609592A (en) * | 2018-11-28 | 2019-04-12 | 云南中烟工业有限责任公司 | A method of detection heating does not burn Cigarette grain phase to the influence of cellular superoxide hydrogenase activity |
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| CN104713752B (en) | 2017-09-22 |
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