CN104316386B - A kind of device for being used to carry out scanning electron microscope example freeze fracture - Google Patents
A kind of device for being used to carry out scanning electron microscope example freeze fracture Download PDFInfo
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- CN104316386B CN104316386B CN201410563142.9A CN201410563142A CN104316386B CN 104316386 B CN104316386 B CN 104316386B CN 201410563142 A CN201410563142 A CN 201410563142A CN 104316386 B CN104316386 B CN 104316386B
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- liquid nitrogen
- sample stage
- cutter
- freeze fracture
- tissue
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Abstract
Liquid nitrogen precooler is insufficient during the application pathology technique with the apparatus field, the existing scanning electron microscope example freeze fracture of solution, easily frostbite, percussion dynamics are uneven, direction is offset, natural section produces not good technical problem accidentally by operating personnel.The technical scheme of use is：The device includes freeze fracture apparatus main body and master station, freeze fracture apparatus main body includes liquid nitrogen tank, sample stage, the fixing device of sample stage both sides, cutter, cutting tool arm and its internal liquid nitrogen EGR and resetting means, knocking device, knocking device arm and its internal resetting means, master station includes secured adjusted device, sample stage adjusting means, percussion dynamics adjusting means, liquid nitrogen loop start device and percussion starter.The technical scheme of the application passes through freeze fracture apparatus main body and master station joint operation, it is ensured that tap direction up rightness and dynamics stability, it is easy to organize the formation of natural section, easy to use, saves manpower, avoids Cryogenic burns, raising from being fabricated to power.
Description
Technical field
The application belongs to pathology technique with the apparatus field, more particularly to a kind of for carrying out freeze fracture to scanning electron microscope example
Device.
Background technology
Freeze fracturing is the sample preparation technology for scanning electron microscopic observation tissue, cell interior fine structure, is passed through
Freeze fracture is carried out in liquid nitrogen, tissue is produced natural section, is easy to observation.At present, conventional method be by metallic plate and
Tissue specimen after fixation is put snap frozen on a metal plate, a hand-held haemostatic clamp folder by blade in advance in liquid nitrogen after precooling
Firmly the blade after precooling, is placed in tissue by blade lower end and treats breaking part, another hand-held mallet taps blade upper end, makes the group of adfreezing
Fast fracture is knitted, natural section is produced, freeze fracture process need to be rapidly completed, it is to avoid the tissue of adfreezing and the blade of precooling are multiple
Temperature.This method metallic plate and blade are both needed to Liquid nitrogen precooler, and operating process needs quickly, to easily cause operating personnel's frostbite, this
Outside, freeze fracture moment, the vertical extent of blade is difficult to grasp with the direction of mallet percussion, dynamics, is easily caused group after fracture
Fragmentation is knitted, enters to splash, lose, and is not easy to the natural section of observation needed for producing, and the operation needs two people to complete simultaneously,
It is very inconvenient.
The content of the invention
A kind of device for being used to carry out scanning electron microscope example freeze fracture, the device includes：
Freeze fracture apparatus main body (1) and master station (9), freeze fracture apparatus main body (1）It is connected with master station (9), it is main
Control platform (9) is connected with external power source and freeze fracture apparatus main body (1) is powered, and freeze fracture apparatus main body (1) includes sample
Platform (2), the fixing device (3) of sample stage (2) both sides, the liquid nitrogen tank (8) below sample stage, cutter (4), cutter (4) with
Liquid nitrogen tank (8) is connected by cutting tool arm (5), cut tool arm (5) inside there is liquid nitrogen EGR (13) and first to reset and fill
Put (15), knocking device (6), knocking device (6) with liquid nitrogen tank (8) by knocking device arm (7) to be connected, knocking device arm
(7) internal to have the second resetting means (17), master station (9) includes secured adjusted device (10), sample stage adjusting means (11), struck
Hit dynamics adjusting means (12), liquid nitrogen loop start device (14), tap starter (16).
Before sample freeze fracture, first poured into liquid nitrogen tank (8) in liquid nitrogen, liquid nitrogen and freeze fracture apparatus main body (1)
Sample stage (2), fixing device (3) contact of sample stage (2) both sides, carry out precooling, while being followed by the liquid nitrogen in master station (9)
Ring starter (14) starts the liquid nitrogen EGR (13) in freeze fracture apparatus main body (1), and liquid nitrogen EGR (13) will
Liquid nitrogen circulation conveying in liquid nitrogen tank (8) reaches the position that cutting tool arm (5) is connected with cutter (4) to tool arm (5) is cut
After turn back defeated time, complete circulation of the liquid nitrogen in cutting tool arm (5), reach the cutter (4) being connected with cutting tool arm (5)
Precooling effect.
Sample stage (2), the fixing device (3) of sample stage (2) both sides in freeze fracture apparatus main body (1), cutter
(4), cutting tool arm (5) is using the good metal material of smooth pieces, oil-stain-preventing, temperature conduction performance.
Liquid nitrogen tank (8) is outside and cutting tool arm (5) is outside using the wrapping of thermal insulation material.
After precooling is complete, tissue sample is put into the center snap frozen of sample stage (2), will wait to be broken when being put into
Face is vertical with the plane of sample stage (2), and parallel with knife face direction.
Sample stage (2) is connected with the sample stage adjusting means (11) of master station (9), can be according to the thickness of tissue when using
Degree, high high adjustment is carried out by sample stage adjusting means (11) to sample stage (2).
Sample stage (2) both sides are equipped with fixing device (3), fixing device (3) and the secured adjusted device (10) of master station (9)
It is connected, according to the size of tissue, length when using, the interval of fixing device (3) is carried out by secured adjusted device (10)
Folding and unfolding is adjusted, and realizes the fixation to tissue.
Fixing device (3) top has the groove design for agreeing with the edge of a knife.
Sample stage (2) and fixing device (3) can accurately be adjusted according to the actual volume of tissue sample, quality hardness
Section.
After tissue sample site secured adjusted is good, is started by the percussion starter (16) of master station (9) and tap dress
Put (6), cutter (4) are produced to the tissue top fixed on sample stage (2) and hung down under the percussion effect of knocking device (6)
Straight external force effect, makes tissue act on natural fracture along this vertical external force, forms the natural section available for observation.
Cutter (4) length is more than the maximum spacing between both sides fixing device (3), to ensure that cutter (4) can only be cut
To the depth of groove.
Knocking device (6) is connected with the percussion dynamics adjusting means (12) of master station (9), taps dynamics adjusting means
(12) there is compass scale, type that can be according to tissue when using, quality hardness are right by tapping dynamics adjusting means (12)
The percussion dynamics of knocking device (6) is adjusted.
Knocking device (6) and coupled knocking device arm (7) are made of the material for not leading temperature.
Planar design is used at the top of cutter (4), with ensure under the percussion effect of knocking device (6) can stress it is equal
It is even.
The one side being in contact at the top of cutter (4) with knocking device (6) has thermal insulation material.
Cut tool arm (5) and knocking device arm (7) has the first resetting means (15) and the second resetting means (17) respectively,
Resetted with meeting automatic rebounding after the completion of percussion.
As a result of the technical scheme of the application, liquid nitrogen is pre- during solving existing scanning electron microscope example freeze fracture
Cold insufficient, operating personnel easily accidentally frostbite, operating process need to be rapidly completed, percussion dynamics is uneven, direction skew, caused to cut
Have no progeny tissue fragmentation, enter to splash, lose and natural section produces not good technical problem.Pass through freeze fracture apparatus main body and master control
The joint operation of platform, it is ensured that tap direction up rightness and dynamics stability, it is easy to organize the formation of natural section, it is to avoid freezing is disconnected
Fragmentation is organized after splitting, enters to splash, lose, by the use of liquid nitrogen tank and liquid nitrogen EGR, realizes and reached in the short time that precooling is imitated
Really, wrapped by outside using thermal insulation material, it is to avoid operating personnel touch accidentally causes liquid nitrogen frostbite.The technical scheme of the application
Flexible and convenient in use, it is easy to adjust, save manpower, avoid Cryogenic burns, improve operating efficiency and sample making quality and
Success rate, and make freeze fracture process more individuation, the need for meeting different observers.
Brief description of the drawings
Shown in figure：
Fig. 1：According to the device for being used to carry out scanning electron microscope example freeze fracture of the application embodiment
Fig. 2：According to the sample stage of the application embodiment and the top view of fixing device
Embodiment
The application provides a kind of device for being used to carry out scanning electron microscope example freeze fracture.The device includes freeze fracture
Apparatus main body (1) and master station (9), freeze fracture apparatus main body (1) are connected with master station (9), master station (9) and external power source
Is connected and freeze fracture apparatus main body (1) is powered, freeze fracture apparatus main body (1) includes sample stage (2), sample stage (2) two
Liquid nitrogen tank (8), cutter (4), cutter (4) below the fixing device (3) of side, sample stage pass through cutting with liquid nitrogen tank (8)
Tool arm (5) is connected, cut tool arm (5) inside have liquid nitrogen EGR (13) and the first resetting means (15), knocking device
(6), knocking device (6) is connected with liquid nitrogen tank (8) by knocking device arm (7), has the second reset inside knocking device arm (7)
Device (17), master station (9) includes secured adjusted device (10), sample stage adjusting means (11), taps dynamics adjusting means
(12), liquid nitrogen loop start device (14), percussion starter (16).
Before sample freeze fracture, first poured into liquid nitrogen tank (8) in liquid nitrogen, liquid nitrogen and freeze fracture apparatus main body (1)
Sample stage (2), fixing device (3) contact of sample stage (2) both sides, carry out precooling, while being followed by the liquid nitrogen in master station (9)
Ring starter (14) starts the liquid nitrogen EGR (13) in freeze fracture apparatus main body (1), and liquid nitrogen EGR (13) will
Liquid nitrogen circulation conveying in liquid nitrogen tank (8) reaches the position that cutting tool arm (5) is connected with cutter (4) to tool arm (5) is cut
After turn back defeated time, complete circulation of the liquid nitrogen in cutting tool arm (5), reach the cutter (4) being connected with cutting tool arm (5)
Precooling effect.Sample stage (2), the fixing device (3) of sample stage (2) both sides in freeze fracture apparatus main body (1), cutter
(4), cutting tool arm (5) is using the metal material design of smooth pieces, oil-stain-preventing, and temperature conduction performance is good, it is easy in short-term
It is interior to reach precooling effect, in addition, liquid nitrogen tank (8) is outside and cutting tool arm (5) outside uses the wrapping of thermal insulation material, it is to avoid behaviour
Make personnel and touch accidentally to cause liquid nitrogen frostbite.
After precooling is complete, tissue sample is put into the center snap frozen of sample stage (2), will wait to be broken when being put into
Face is vertical with the plane of sample stage (2), and parallel with knife face direction.The sample stage adjusting means of sample stage (2) and master station (9)
(11) it is connected, high low-key can be carried out to sample stage (2) by sample stage adjusting means (11) according to the thickness of tissue when using
Section, it is ensured that being organized in during freeze fracture can be acted on by the external force of cutter (4).Sample stage (2) both sides are equipped with fixation
Device (3), fixing device (3) is connected with the secured adjusted device (10) of master station (9), can be according to the big of tissue when using
Small, length, carries out folding and unfolding regulation to the interval of fixing device (3) by secured adjusted device (10), realizes the fixation to tissue,
Make to be organized in during freeze fracture by external force effect it is excessive when will not fragmentation, enter to splash and position changes and causes section inclined
Move.Fixing device (3) top has the groove design for agreeing with the edge of a knife, has stop cushioning effect to cutter (4), it is to avoid knife edge mistake
Degree damage, and be prevented effectively to organizing the full-cutting cut surface for being formed with tool marks, it is beneficial to tissue under external force
Natural fracture, forms natural section.Sample stage (2) is respectively provided with controllability with fixing device (3), can be according to tissue sample
Actual volume, quality hardness carry out accurate adjustment, and flexible and convenient makes tissue cut off process more individuation, to meet different sights
The need for the person of examining.
After tissue sample site secured adjusted is good, is started by the percussion starter (16) of master station (9) and tap dress
Put (6), cutter (4) are produced to the tissue top fixed on sample stage (2) and hung down under the percussion effect of knocking device (6)
Straight external force effect, makes tissue act on natural fracture along this vertical external force, forms the natural section available for observation.Cutter (4)
Length is more than the maximum spacing between both sides fixing device (3), to ensure that cutter (4) can only switch to the depth of groove, it is to avoid
It is full-cutting to organizing during cutting off, it is formed with the cut surface rather than natural section of tool marks.Knocking device (6) and master
The percussion dynamics adjusting means (12) of control platform (9) is connected, and tapping dynamics adjusting means (12) has compass scale, can when using
According to the type of tissue, quality hardness, the percussion dynamics of knocking device (6) is adjusted by tapping dynamics adjusting means (12)
Section, knocking device (6) and coupled knocking device arm (7) are made of the material for not leading temperature, save liquid nitrogen.Cutting
Planar design is used at the top of knife (4), with ensure percussion effect in knocking device (6) it is lower can uniform force, cutter (4) push up
The one side that portion is in contact with knocking device (6) has thermal insulation material, to prevent knocking device (6) and cutter (4) excessive temperature differentials,
The low temperature of cutter (4) is impacted during percussion, cutter (4) are designed using smooth pieces, oil-stain-preventing metal material,
It is easy to temperature conduction, is easy to wipe and clears up, it is to avoid tissue sample pollutes, meanwhile, cutter (4) interchangeable bit, it is to avoid due to
For a long time using caused knife edge rust, tissue, which is cut off, to be obstructed, and natural section is not good.Cutter according to the sharpness of the knife edge,
Change with plurality of specifications is available, this porous class loading of lung is organized as if waiting to cut off, the slightly blunt cutting of the optional knife edge
Knife, the finer and close tissue of this quality of liver is organized as if waiting to cut off, and can select the knife edge slightly sharp cutter, and cutter can be according to
Factually border organization type, quality carry out replacing selection, are beneficial to carry out individuation freeze fracture to tissue specimen, improve sample
Quality is made, the observation requirementses of different specimens are met, in addition, cutting tool arm (5) and knocking device arm (7) inner upper end corner
There is the first resetting means (15) and the second resetting means (17) respectively, resetted with meeting automatic rebounding after the completion of percussion.Cutting
The use of knife (4) and knocking device (6) makes can possess stable vertical external force effect during tissue freeze fracture, is easy to group
Natural fracture is knitted, tissue is cut off process more mechanization, it is to avoid a variety of artificial uncertainties are to cutting off the shadow that effect is caused
Ring, improve preparation of specimen's quality.
Claims (2)
1. a kind of device for being used to carry out scanning electron microscope example freeze fracture, the device includes：
Freeze fracture apparatus main body (1) and master station (9), freeze fracture apparatus main body (1) are connected with master station (9), master station
(9) is connected with external power source and freeze fracture apparatus main body (1) is powered, freeze fracture apparatus main body (1) includes sample stage
(2), the fixing device (3) of sample stage (2) both sides, liquid nitrogen tank (8), cutter (4) and knocking device (6) below sample stage；
The cutter (4) are connected with liquid nitrogen tank (8) by cutting tool arm (5), have liquid nitrogen EGR inside cutting tool arm (5)
And the first resetting means (15) (13)；The knocking device (6) is connected with liquid nitrogen tank (8) by knocking device arm (7), is tapped
There is the second resetting means (17) inside devices arms (7)；Master station (9) includes secured adjusted device (10), sample stage adjusting means
(11) dynamics adjusting means (12), liquid nitrogen loop start device (14), are tapped, starter (16) is tapped；
Before sample freeze fracture, liquid nitrogen, liquid nitrogen and the sample in freeze fracture apparatus main body (1) are first poured into liquid nitrogen tank (8)
Platform (2), fixing device (3) contact of sample stage (2) both sides, carry out precooling, while passing through the liquid nitrogen circulation open in master station (9)
Dynamic device (14) starts the liquid nitrogen EGR (13) in freeze fracture apparatus main body (1), and liquid nitrogen EGR (13) is by liquid nitrogen
Liquid nitrogen circulation conveying in groove (8) reached and rolled over behind the position that cutting tool arm (5) is connected with cutter (4) to tool arm (5) is cut
Return defeated time, complete circulation of the liquid nitrogen in cutting tool arm (5), make to reach precooling with the cutter (4) that cutting tool arm (5) is connected
Effect；
Sample stage (2), the fixing device (3) of sample stage (2) both sides, cutter (4) in freeze fracture apparatus main body (1), cut
Cutter arm (5) is using the good metal material of smooth pieces, oil-stain-preventing, temperature conduction performance；
Liquid nitrogen tank (8) is outside and cutting tool arm (5) is outside using the wrapping of thermal insulation material；
After precooling is complete, tissue sample is put into the center snap frozen of sample stage (2), will be treated when being put into the plane of disruption with
The plane of sample stage (2) is vertical, and parallel with knife face direction；
Sample stage (2) is connected with the sample stage adjusting means (11) of master station (9), can be led to when using according to the thickness of tissue
Cross sample stage adjusting means (11) and high high adjustment is carried out to sample stage (2)；
Sample stage (2) both sides are equipped with fixing device (3), and fixing device (3) is connected with the secured adjusted device (10) of master station (9)
Connect, according to the size of tissue, length when using, folding and unfolding is carried out to the interval of fixing device (3) by secured adjusted device (10)
Regulation, realizes the fixation to tissue；
Fixing device (3) top has the groove design for agreeing with the edge of a knife；
Sample stage (2) and fixing device (3) can carry out accurate adjustment according to the actual volume of tissue sample, quality hardness；
After tissue sample site secured adjusted is good, knocking device is started by the percussion starter (16) of master station (9)
(6), cutter (4) are produced vertical under the percussion effect of knocking device (6) to the tissue top fixed on sample stage (2)
External force is acted on, and tissue is acted on natural fracture along this vertical external force, is formed the natural section available for observation；
Cutter (4) length is more than the maximum spacing between both sides fixing device (3), to ensure that it is recessed that cutter (4) can only be switched to
The depth of groove；
Knocking device (6) is connected with the percussion dynamics adjusting means (12) of master station (9), taps dynamics adjusting means (12) tool
There is compass scale, type that can be according to tissue when using, quality hardness are filled by tapping dynamics adjusting means (12) to tapping
The percussion dynamics for putting (6) is adjusted；
Knocking device (6) and coupled knocking device arm (7) are made of the material for not leading temperature；
Planar design is used at the top of cutter (4), so that ensure being capable of uniform force under the percussion effect of knocking device (6)；
The one side being in contact at the top of cutter (4) with knocking device (6) has thermal insulation material；
Cut tool arm (5) and knocking device arm (7) has the first resetting means (15) and the second resetting means (17) respectively, with full
Automatic rebounding resets after the completion of foot is tapped.
2. the device as claimed in claim 1 for being used to carry out scanning electron microscope example freeze fracture, wherein cutter (4) are can
Cutter head is changed, according to the sharpness of the knife edge, with the available replacing of plurality of specifications, porous class group is organized as if waiting to cut off
Knit, from the knife edge slightly blunt cutter, the finer and close tissue of quality is organized as if waiting to cut off, from the knife edge slightly sharp cutting
Knife.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410563142.9A CN104316386B (en) | 2014-10-22 | 2014-10-22 | A kind of device for being used to carry out scanning electron microscope example freeze fracture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410563142.9A CN104316386B (en) | 2014-10-22 | 2014-10-22 | A kind of device for being used to carry out scanning electron microscope example freeze fracture |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104316386A CN104316386A (en) | 2015-01-28 |
| CN104316386B true CN104316386B (en) | 2017-07-18 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410563142.9A Expired - Fee Related CN104316386B (en) | 2014-10-22 | 2014-10-22 | A kind of device for being used to carry out scanning electron microscope example freeze fracture |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106404511B (en) * | 2016-08-31 | 2018-10-09 | 山东山大附属生殖医院有限公司 | Biological sample materials refrigerating plant |
| CN106483693B (en) * | 2016-12-30 | 2019-12-31 | 深圳市华星光电技术有限公司 | Device and method for detecting liquid crystal state in liquid crystal panel |
| CN106814095B (en) * | 2017-02-12 | 2019-05-24 | 马新军 | Freezing sample preparation device for scanning electron microscope |
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|---|---|---|---|---|
| US4336691A (en) * | 1979-12-13 | 1982-06-29 | The Board Of Trustees Of The Leland Stanford Junior University | Cryojet rapid freezing apparatus |
| CN2268492Y (en) * | 1996-09-11 | 1997-11-26 | 李朝林 | Semiconductor constant frozen sheet cutter |
| CN101665236A (en) * | 2009-09-08 | 2010-03-10 | 北京航空航天大学 | Controllable temperature sample table with controllable temperature range of 77K to 400K |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3781723B2 (en) * | 2002-12-20 | 2006-05-31 | 日本電子株式会社 | Sample holder for freezing breakage of high-pressure freezing apparatus and high-pressure freezing apparatus |
-
2014
- 2014-10-22 CN CN201410563142.9A patent/CN104316386B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4336691A (en) * | 1979-12-13 | 1982-06-29 | The Board Of Trustees Of The Leland Stanford Junior University | Cryojet rapid freezing apparatus |
| CN2268492Y (en) * | 1996-09-11 | 1997-11-26 | 李朝林 | Semiconductor constant frozen sheet cutter |
| CN101665236A (en) * | 2009-09-08 | 2010-03-10 | 北京航空航天大学 | Controllable temperature sample table with controllable temperature range of 77K to 400K |
Non-Patent Citations (1)
| Title |
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| 不同固定方法对植物细胞扫描电镜观察用冷冻断裂样品的影响;胡阿林 等;《植物学报》;19900615;第32卷;第438-441页 * |
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| CN104316386A (en) | 2015-01-28 |
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