CN104224117B - It is a kind of optical spectrum encoded confocal imaging method and system to be cooperateed with optical coherence tomography - Google Patents
It is a kind of optical spectrum encoded confocal imaging method and system to be cooperateed with optical coherence tomography Download PDFInfo
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Abstract
Confocal imaging method and system are cooperateed with optical coherence tomography the invention discloses one kind is optical spectrum encoded, the system is based on the combination of optical coherence tomography (OCT) imaging technique and optical spectrum encoded confocal microscopy (SECM) technology, the sample arm by OCT systems for proposing shares same light path with the sample arm of SECM systems, so that being synchronously scanned, and the light returned from sample scattering,single is respectively through respective optic path to feeler arm to sample same position from the light beam of OCT systems and SECM system exits in sample arm.The feeler arm of proposition uses two fiber optic collimator mirrors to irradiate grating with different incidence angles, optical grating diffraction is set to go out two groups of spectral signals of no overlap outgoing, the signal of as SECM and OCT, the light path of the sample arm in the present invention is simpler, it is convenient to carry out, light path is also compacter, stabilization, and the image quality of system is high, mechanics of biological tissue is imaged more accurate.
Description
Technical field
The present invention relates to optical spectrum encoded confocal microscopy and Optical Coherence Tomography Imaging Technology, more particularly to a kind of spectrum
Encoded confocal cooperates with the technology of synchronous imaging with optical coherence tomography.
Background technology
Optical coherent chromatographic imaging (Optical Coherence Tomography, abbreviation OCT) technology is a kind of emerging
Biomedical optical imaging technique, can realize carrying out noncontact, not damaged, high score to the structure of biological tissue and physiological function
Resolution is imaged, and is had broad application prospects in the early detection of disease and in body biopsy field.
Optical spectrum encoded confocal microscopy (Spectrally Encoded Confocal Microscopy, abbreviation SECM) technology
It is a kind of confocal imaging method of minimally invasive single-mode fiber, the technology is realized detecting sample simultaneously using wideband light source and diffraction grating
Reflectivity at the point of product multiple lateral attitude.By detecting the spatial information of sample arm, pumped FIR laser confocal microscope system can in order to avoid
Except mechanical scanning, there is provided resolution ratio tends to the vivo biological tissue image of electronmicroscopic level, its compact nature can be realized
It is fitted into small diameter conduits or endoscopic catheters.
Comparatively speaking, the imaging depth of OCT technology is greater than SECM technologies.OCT technology can provide axial resolution high
Biological tissue's section tomographic map, SECM technologies can provide the biological tissue superficial layer cross-sectional view of high lateral resolution
Picture, the advantage of two kinds of technologies in terms of spatial resolution complements one another, therefore OCT technology can with the imaging that cooperates with of SECM technologies
More high spatial resolution and more comprehensive biological tissue's microscopy information are provided, are examined in biomedical imaging and non-biological material
To all have great importance in the application such as survey.
It is mostly the pendulum based on scanning galvanometer in the method that the OCT systems for being proposed at present are imaged with SECM systematic collaborations
Move to realize that the switch between two kinds of systems switches, this use galvanometer is complex as the system architecture of mechanical switch, and
And scanning imagery while not accomplishing truly, if sample is biopsy samples, just probably appears in and cut
The situation of the two systems image space misalignment caused because live body is moved during changing.In addition, at present being proposed
In the method that OCT systems are imaged with SECM systematic collaborations, OCT systems and SECM systems are respectively used to using two light sources mostly,
And additionally use two feeler arms and separately detect the sample signal of OCT systems and the sample signal of SECM systems so that integrate
System afterwards is excessively complicated, is not sufficiently stable, and builds relatively costly.Therefore, how in the case where light path is relatively simple, design
Go out it is a kind of it is optical spectrum encoded it is confocal cooperate with imaging method and system with optical coherence tomography, realization synchronous scanning truly into
Picture, while reducing the quantity of light source and feeler arm, makes the collaboration compacter stabilization of imaging system structure, just turn into OCT systems with
The big target that SECM systematic collaborations imaging system is developed.
The content of the invention
Goal of the invention：In order to overcome the deficiencies in the prior art, a kind of optical spectrum encoded confocal and optical coherence is proposed
Chromatography collaboration imaging method and system.This is optical spectrum encoded confocal to cooperate with imaging method and system to use light with optical coherence tomography
The sample arm of coherence chromatographic imaging (OCT) system shares same light with the sample arm of optical spectrum encoded confocal microscopy (SECM) system
Road so that in sample arm from the light beam of OCT systems and SECM system exits to sample same position synchronously be scanned, from sample
The light that scattering,single is returned respectively through two fiber optic collimator mirrors in respective optic path to feeler arm, by by the two light
Fine collimating mirror irradiates grating with different incidence angles, optical grating diffraction is gone out two groups of spectral signals of no overlap outgoing, as SECM and
The signal of OCT, by amasthenic lens, focuses on the left-half pixel and right side of linear charge-coupled array (CCD) respectively
Divide pixel, that is, realize detecting the spectral signal from SECM and OCT systems simultaneously.Data processing acquisition is carried out through incoming computer
The OCT image of sample axial resolution high and the SECM images of high lateral resolution, realize that OCT systems are excellent with SECM systems
The complementary collaboration imaging of gesture.
To achieve the above object, the technical solution adopted by the present invention is：A kind of optical spectrum encoded confocal and optical coherence tomography
Collaboration imaging method, same light path is shared by the sample arm of the sample arm of OCT systems and SECM systems so that in sample arm from
The light beam of OCT systems and SECM system exits to sample same position synchronously be scanned, from the light that sample scattering,single is returned
Respectively through two fiber optic collimator mirrors in respective optic path to feeler arm, entered with difference by by the two fiber optic collimator mirrors
Firing angle irradiates grating, optical grating diffraction is gone out two groups of spectral signals of no overlap outgoing, and the spectral signal is the letter of SECM and OCT
Number, by amasthenic lens, the left-half pixel and right half part pixel of linear charge-coupled array are focused on respectively, that is, realize
The spectral signal from SECM and OCT systems is detected simultaneously.
It is a kind of that imaging system, including light are cooperateed with optical coherence tomography based on optical spectrum encoded confocal described in claim 1
Source (1), reference arm (2), sample arm (3), feeler arm (4), Transmission Fibers (5), the first optoisolator (6), the second optoisolator
(7), the first fiber coupler (8) and the second fiber coupler (9), one end of first fiber coupler (8) passes through respectively
Transmission Fibers (5) connect light source (1) and feeler arm (4) respectively, and the other end connects sample arm respectively by Transmission Fibers (5)
(3) and the second fiber coupler (9) one end, and be provided with the first light between the light source (1) and the first fiber coupler (8)
Isolator (6), is connected to the second optoisolator (7) between the first fiber coupler (8) and the second fiber coupler (9)；Described
One end that two fiber couplers (9) are connected with the first fiber coupler (8) is also connected with feeler arm (4), and second optical fiber
The other end of coupler (9) connects reference arm (2) and sample arm (3) respectively.
The sample arm (3) including set gradually by light flow speed and direction the first fiber optic collimator mirror (10), the first grating (11),
First convex lens (12), the second convex lens (13) and microcobjective (16), first fiber coupler (8) and the second optical fiber
Coupler (9) connection one end be connected with the first fiber optic collimator mirror (10), and first grating (11) spot center, first
Convex lens (12) center, the second convex lens (13) center and microcobjective center are on same optical axis；With the first convex lens
(12) it is Z axis with the second convex lens (13) optical axis line direction, is connected with the optical axis of the second fiber optic collimator mirror (14) and galvanometer (15)
Line direction is Y-axis, and the center with the first convex lens is origin, according to the right-hand rule, sets up coordinate system；First grating (11)
Surface is perpendicular to YZ planes, and first grating (11) and the angle of XZ are a；Also include being set gradually by beam propagation order
The second fiber optic collimator mirror (14) and galvanometer (15), one end and the of second fiber coupler (9) and reference arm (2) connection
Two fiber optic collimator mirrors (14) are connected, and the galvanometer (15) is arranged between the first grating (11) and the first convex lens (12), described
Galvanometer (15) surface perpendicular to YZ planes, and galvanometer (15) and the second fiber optic collimator mirror (14) height in same level, separately
Outer second fiber optic collimator mirror (14) and galvanometer (15) are below the hot spot on the first grating (11) along the skew of positive X-direction
Amount>0；Light beam from the first fiber coupler (8) is projected the first grating (11) table by first fiber optic collimator mirror (10)
Hot spot is formed on face, the spectrum gone out from the first optical grating diffraction is incident upon the top half of the first convex lens (12)；Second optical fiber is accurate
Be incident upon light beam from the second fiber coupler (9) on galvanometer (15) by straight mirror (14), and the light beam reflects by galvanometer (15)
In the latter half of the first convex lens (12), by the sweep limits for adjusting the first grating (11) placed angle a and galvanometer (15)
So that the scanning angular region from the light beam of galvanometer (15) reflection is equal with the angle dispersion scope of the first grating (11) emergent light；Projection
The first convex lens (12) the light beam of two parts up and down by after the first convex lens (12), it is burnt in the image space of the first convex lens (12)
Flat focus are into the bright line spot of same horizontal direction, and this bright line spot is vertical with the optical axis of the first convex lens (12) in midpoint
It is orthogonal, and continue to incide on the second convex lens (13)；Assembled from the light beam of the second convex lens (13) outgoing, and all entered
It is mapped in microcobjective (16), the light beam focused on by microcobjective (16) is incident upon on sample；Returned from sample scattering,single
Light returned through original optical path and return to respective light respectively through the first fiber optic collimator mirror (10), the second fiber optic collimator mirror (14)
Road, and enter feeler arm.
The feeler arm (4) including the 3rd fiber optic collimator mirror (17), the 4th fiber optic collimator mirror (18), the second grating (19),
Amasthenic lens (20) and linear charge-coupled array (21)；First fiber coupler (8) and light source (1) connection one end with
4th fiber optic collimator mirror (18) connect, and second fiber coupler (9) and the first fiber coupler (8) connection one end and
3rd fiber optic collimator mirror (17) is connected；3rd fiber optic collimator mirror (17) and the 4th fiber optic collimator mirror (18) are in the second grating
(19) the same side, and the 3rd fiber optic collimator mirror (17) and the 4th fiber optic collimator mirror (18) respectively with the second grating (19) into
Different angles is placed；And the second grating (19) opposite side places linear charge-coupled array (21), the amasthenic lens
(20) it is placed between the second grating (19) and linear charge-coupled array (21)；3rd fiber optic collimator mirror (17) is collected and
From in the light beam of the first fiber coupler (8), and the 4th fiber optic collimator mirror (18) is collected and comes from the second fiber coupler
(9) light beam, and the 3rd fiber optic collimator mirror (17), the 4th fiber optic collimator mirror (18) are respectively with different incident angles by two
Individual light beam is projected on the second grating (19), the second grating (19) diffraction is gone out two groups of spectral signals of no overlap outgoing, as
The signal of SECM and OCT, by amasthenic lens (20), focus on respectively line array CCD (21) photosurface left-half pixel and
Right half part pixel.
Preferably：The distance between first convex lens (12) and second convex lens (13) for two lens focal length it
With the second convex lens (13) are equal to the focal length of the second convex lens (13) with the distance of microcobjective (16).Two groups of spectrum letter
Focus on CCD photosurfaces to number no overlap.
The a kind of optical spectrum encoded confocal of present invention offer cooperates with imaging method and system with optical coherence tomography, compared to existing
Technology, has the advantages that：
1st, by using by the sample of the sample arm of optical coherence tomography system and optical spectrum encoded confocal microscope system
Arm shares same light path so that the light beam in sample arm from OCT systems and SECM system exits is to the same stepping of sample same position
Row scanning, effectively overcomes the problem brought using mechanical switch during by two system combinations, has been truly realized synchronous scanning sample
Product.In addition, the method and system compared to before, the light path of the sample arm in the present invention is simpler, convenient to carry out, and light path is also more
Step up to gather, stablize.
2nd, confocal imaging method and system are cooperateed with optical coherence tomography by using optical spectrum encoded so that the axle of the system
There is large increase relative to single SECM systems to resolution ratio, and make the lateral resolution of the system relative to single
OCT systems are greatly improved, and greatly optimize the image quality of system.
3rd, by using by the sample of the sample arm of optical coherence tomography system and optical spectrum encoded confocal microscope system
Arm shares same light path, it is only necessary to use a light source；In addition, being compiled by using by optical coherence tomography system and spectrum
Code confocal microscope system realizes the light path of detection simultaneously in feeler arm so that OCT systems are realized detecting simultaneously with SECM systems
Imaging, this detection method is eliminated while being carried out the trouble of detectable signal using two feeler arms, it is only necessary to a feeler arm
The spectral signal of OCT systems and SECM systems is detected simultaneously so that system architecture is compacter, light path is more stablized, and saves
About build cost.
4th, confocal imaging method and system are cooperateed with optical coherence tomography by using optical spectrum encoded so that SECM systems with
The imaging results of OCT systems mutually registration, pixel alignment, so that the system is more accurate to mechanics of biological tissue imaging.
Brief description of the drawings
Fig. 1 is structural representation of the invention；
Fig. 2 is the schematic diagram of sample arm；
Fig. 3 is the schematic diagram of feeler arm；
In figure：1st, light source, 2, reference arm, 3, sample arm, 4, feeler arm, 5, Transmission Fibers, the 6, first optoisolator, 7,
Two optoisolators, the 8, first fiber coupler, the 9, second fiber coupler, the 10, first fiber optic collimator mirror, the 11, first grating,
12nd, the first convex lens, the 13, second convex lens, the 14, second fiber optic collimator mirror, 15, galvanometer, 16, microcobjective, the 17, the 3rd optical fiber
Collimating mirror, the 18, the 4th fiber optic collimator mirror, the 19, second grating, 20, amasthenic lens, 21, linear charge-coupled array (CCD).
Specific embodiment
The present invention is further illustrated with reference to the accompanying drawings and examples, and the purpose of the present invention and effect will become more
Plus substantially.
It is a kind of it is optical spectrum encoded it is confocal imaging method is cooperateed with optical coherence tomography, as Figure 1-3, by the sample of OCT systems
Product arm shares same light path with the sample arm of SECM systems so that from OCT systems and the light beam of SECM system exits in sample arm
Sample same position is synchronously scanned, the light from the return of sample scattering,single is respectively through respective optic path to feeler arm
In two fiber optic collimator mirrors, by by the two fiber optic collimator mirrors with different incidence angles irradiate grating, optical grating diffraction is gone out two
The spectral signal of group no overlap outgoing, the spectral signal is the signal of SECM and OCT, by amasthenic lens, focuses on respectively
The left-half pixel and right half part pixel of linear charge-coupled array, that is, realize that detection comes from SECM and OCT systems simultaneously
Spectral signal.
It is a kind of that imaging system is cooperateed with optical coherence tomography based on optical spectrum encoded confocal described in claim 1, such as Fig. 1 institutes
Show, including light source 1, reference arm 2, sample arm 3, feeler arm 4, Transmission Fibers 5, the first optoisolator 6, the second optoisolator 7,
Distinguished by Transmission Fibers 5 respectively one fiber coupler 8 and the second fiber coupler 9, one end of first fiber coupler 8
Connection light source 1 and feeler arm 4, and the other end connects the one of the fiber coupler 9 of sample arm 3 and second respectively by Transmission Fibers 5
End, and the first optoisolator 6, the first fiber coupler 8 and second are provided between the fiber coupler 8 of the light source 1 and first
The second optoisolator 7 is connected between fiber coupler 9；Second fiber coupler 9 be connected with the first fiber coupler 8 one
End is also connected with feeler arm 4, and the other end of second fiber coupler 9 connects reference arm 2 and sample arm 3 respectively.
The broadband light that light source 1 sends is transmitted to the first fiber coupler 8 by the optoisolator 6 of Transmission Fibers 5 and first, and
It is divided into the first fiber optic collimator mirror 10 and the second optoisolator 7 that two-beam is transmitted separately in sample arm 3.It is accurate from the first optical fiber
The light beam of the straight incidence of mirror 10 is reflected through sample arm 3, again passes by the first fiber coupler 8 and Transmission Fibers 5 are transmitted to spy
The 4th collimating mirror 18 in test arm 4 is simultaneously detected.Light beam from the outgoing of the second optoisolator 7 is by the second fiber coupler 9
And it is divided into two parts, a part of light beam is into reference arm 2 and reflects, and another part light beam enters the second of sample arm 3
Fiber optic collimator mirror 14 and reflecting by sample arm 3, the two-beam for reflecting are coupled simultaneously by the second fiber coupler 9
Interfere, interference light is by the 3rd fiber optic collimator mirror 17 in the incident feeler arm 4 of Transmission Fibers 5 and enters spy in feeler arm
Survey.
Reference arm 2 is used to provide reference optical signal.It is typical that reference arm is by fiber optic collimator mirror, convex lens and is fixed on translation
Speculum group on platform into.
Optoisolator is a kind of magneto-optic device that can be absorbed or offset reverse transfers.Fibre optic isolater is generally used to protect
Shield light source prevents the optical damage that intensity noise causes from the influence of back reflection.Typical optoisolator parameter is referred to
The optoisolator of the Thorlabs companies production in the U.S..
Fiber coupler is a kind of element for realizing optical signal branch or combining.Typical optoisolator parameter can join
Examine the fiber coupler of the Thorlabs companies production in the U.S..
As shown in Fig. 2 the sample arm 3 includes the first fiber optic collimator mirror 10, the first light that are set gradually by light flow speed and direction
Grid 11, the first convex lens 12, the second convex lens 13 and microcobjective 16, the optical fiber coupling of first fiber coupler 8 and second
One end of the connection of clutch 9 is connected with the first fiber optic collimator mirror 10, and the spot center of first grating 11, the first convex lens 12
Center, the center of the second convex lens 13 and microcobjective center are on same optical axis；With the first convex lens 12 and the second convex lens
The optical axis line direction of mirror 13 is Z axis, and the optical axis line direction with the second fiber optic collimator mirror 14 and galvanometer 15 is Y-axis, i.e., such as Fig. 2 institutes
Show, vertical paper direction and perpendicular to the direction of the first convex lens 12 and the line of centres of the second convex lens 13 be Y-axis, it is convex with first
The center of lens is origin, according to the right-hand rule, sets up coordinate system；The surface of first grating 11 is perpendicular to YZ planes, and institute
The angle that the first grating 11 is stated with XZ is a；Also include the second fiber optic collimator mirror 14 set gradually by beam propagation order and shake
One end of mirror 15, second fiber coupler 9 and the connection of reference arm 2 is connected with the second fiber optic collimator mirror 14, the galvanometer 15
It is arranged between the first grating 11 and the first convex lens 12, the surface of the galvanometer 15 is perpendicular to YZ planes, and galvanometer 15 and second
, in same level, second fiber optic collimator mirror 14 and galvanometer 15 are on the first grating 11 in addition for the height of fiber optic collimator mirror 14
Hot spot lower section along positive X-direction side-play amount>0；
First fiber optic collimator mirror 10 projects on the surface of the first grating 11 light beam from the first fiber coupler 8
Hot spot is formed, the spectrum gone out from the first optical grating diffraction is incident upon the top half of the first convex lens 12；Second fiber optic collimator mirror 14
Light beam from the second fiber coupler 9 is incident upon on galvanometer 15, the light beam is reflected in the first convex lens 12 by galvanometer 15
The latter half, the light for causing to be reflected from galvanometer 15 by the sweep limits for adjusting the placed angle a of the first grating 11 and galvanometer 15
The scanning angular region of beam is equal with the angle dispersion scope of the emergent light of the first grating 11.It is incident upon two up and down of the first convex lens 12
Divided beams is by after the first convex lens 12, the bright line of same horizontal direction being focused into the image space focal plane of the first convex lens 12
Spot, this bright line spot continues to incide on the second convex lens 13 in midpoint and the optical axis perpendicular quadrature of the first convex lens 12；
Assembled from the light beam of the outgoing of the second convex lens 13, and all incided microcobjective 16, focused on by microcobjective 16
Light beam be incident upon on sample；The light returned from sample scattering,single is returned and respectively through the first fiber optic collimator mirror through original optical path
10th, the second fiber optic collimator mirror 14 returns to respective light path, and enters feeler arm.The convex lens 13 of first convex lens 12 and second
The distance between be the focal length sum of two lens, the second convex lens 13 are equal to the second convex lens 13 with the distance of microcobjective 16
Focal length.Focus on CCD photosurfaces to two groups of spectral signal no overlaps.
Specifically：First fiber optic collimator mirror 10 is collected the light beam from optical spectrum encoded confocal microscopy SECM systems and is thrown
It is mapped on the first grating 11, the spectrum gone out from the first optical grating diffraction is incident upon the top half of the first convex lens 12, the second optical fiber
Collimating mirror 14 collects the light beam from optical coherent chromatographic imaging OCT systems and the reflection by galvanometer 15 is incident upon the first convex lens
The latter half of mirror 12, causes what is reflected from galvanometer 15 by the sweep limits for adjusting the placed angle of the first grating 11 and galvanometer 15
The scanning angular region of light beam is equal with the angle dispersion scope of the emergent light of the first grating 11.It is incident upon up and down the two of the first convex lens 12
Segment beam is focused into the bright of horizontal direction by after the first convex lens 12 in the image space focal plane of the first convex lens 12
Line, in midpoint and the optical axis perpendicular quadrature of the first convex lens 12, two-beam is coupled this bright line since then, and continues incidence
To in the second convex lens 13.Restrained when comparing incident first convex lens 12 from the light beam of the outgoing of the second convex lens 13, and entirely
Portion is incided in microcobjective 16, and the light beam focused on by microcobjective 16 is incident upon on sample.Returned from sample scattering,single
Light returned through original optical path and respectively through the first fiber optic collimator mirror 10, the second fiber optic collimator mirror 14 return to respective light path, and
Into feeler arm.
Grating can make light beam that dispersion occurs, and the light of different wave length is beaten on sample successively, optical spectrum encoded so as to realize.
Grating can be divided into transmissive diffraction grating and reflective diffraction gratings, and typical grating parameter refers to the Wasatch in the U.S.
The grating of Photonics companies production.
Fiber optic collimator mirror is accurately positioned with GRIN Lens by tail optical fiber and formed, the diverging light transformation that it can send optical fiber
Into the parallel spatial light of collimation.
The drive signal that galvanometer 15 can send according to computer controller, makes its optical scanning head realize pivoting, from
And cause that the light beam from optical scanning head reflection is scanned in a plane.It is usually used in being realized in the sample arm of OCT systems
Scanning of the light beam to sample various location.
Microcobjective 16 can focus on the beam heights of sample arm, and the luminous point for making to get on sample is smaller, so that being
The lateral resolution of system is improved.
As shown in figure 3, the feeler arm 4 includes the 3rd fiber optic collimator mirror 17, the 4th fiber optic collimator mirror 18, the second grating
19th, amasthenic lens 20 and linear charge-coupled array 21；First fiber coupler 8 and one end and the 4th of the connection of light source 1
Fiber optic collimator mirror 18 is connected, and one end of 9 and first fiber coupler of the second fiber coupler 8 connection is accurate with the 3rd optical fiber
Straight mirror 17 is connected；3rd fiber optic collimator mirror 17 and the 4th fiber optic collimator mirror 18 are in the same side of the second grating 19 and described
3rd fiber optic collimator mirror 17 and the 4th fiber optic collimator mirror 18 angle different from the second 19 one-tenth of grating respectively are placed；And described second
The opposite side of grating 19 places linear charge-coupled array 21, and the amasthenic lens 20 is placed on the second grating 19 and linear array electric charge coupling
Between clutch part 21.
3rd fiber optic collimator mirror 17 collects the light beam for coming from the first fiber coupler 8, and the 4th optical fiber is accurate
Straight mirror 18 collects the light beam for coming from the second fiber coupler 9, and the 3rd fiber optic collimator mirror 17, the 4th fiber optic collimator mirror 18
Two light beams are projected on the second grating 19 with different incident angles respectively, the diffraction of the second grating 19 is gone out two groups of no overlaps
The signal of the spectral signal of outgoing, as SECM and OCT, by amasthenic lens 20, focuses on the photosurface of line array CCD 21 respectively
Left-half pixel and right half part pixel.
Specifically：3rd fiber optic collimator mirror 17, the 4th fiber optic collimator mirror 18 collect the light of OCT systems and SECM systems respectively
Beam, and projected on the second grating 19 light beam from two systems with different incident angles, by calculating and adjusting this
Two sizes of incidence angle, make the diffraction of the second grating 19 go out two groups of spectral signals of no overlap outgoing, the letter of as SECM and OCT
Number, and one of which spectral signal the corresponding angle of emergence of maximum wavelength it is corresponding with the minimum wavelength of another group of spectral signal go out
Firing angle is equal, and so this two groups of spectral signals are propagated in the space of the right and left respectively, and two groups of spectral signals are tightly adjacent,
In then projecting amasthenic lens 20.By the focusing of amasthenic lens 20 so that light of this two groups of spectral signals in linear array CCD21
A bright line is focused on quick face, left and right one side of something of this bright line is respectively the spectral signal of SECM systems and OCT systems, i.e.,
The left-half and right half part of the photosurface of line array CCD 21 separately detect the spectral signal of SECM systems and OCT systems.
Light intensity signal can be converted into voltage signal by line array CCD 21 in real time, and voltage signal is passed through data transmission cable
It is defeated to be acquired to computer.Typical line array CCD parameter refers to the line array CCD (Aviiva of French Atmel companies production
SM2).Compared with traditional picture pick-up device, line array CCD 21 has that spectral response is wide, good linearity, wide dynamic range, noise are low, spirit
Many advantages such as sensitivity is high, real-time Transmission and electric charge self-scanning, be widely used at present remotely sensed image, satellite monitoring,
The fields such as machine vision.
One kind disclosed by the invention is optical spectrum encoded confocal to cooperate with imaging method and system, the general of proposition with optical coherence tomography
The sample arm of optical coherence tomography collaboration (OCT) imaging system shares same with the sample arm of optical spectrum encoded confocal (SECM) system
Light path so that in sample arm from the light beam of OCT systems and SECM system exits to sample same position synchronously be scanned, and
The light returned from sample scattering,single returns to respective light path and returns to feeler arm respectively, and this optical coupled method effectively overcomes
The problem brought using mechanical switch during by two system combinations, has been truly realized the same position of synchronous scanning sample.This
It is kind optical spectrum encoded confocal imaging method and system to be cooperateed with optical coherence tomography so that the axial resolution of the system relative to
SECM systems have large increase, and its lateral resolution is greatly improved relative to OCT systems, optimize system
Image quality.In addition, the method and system compared to before, the light path of the sample arm in the present invention is simpler, convenient to carry out, and
And only need to, using a light source, save cost and simplified system architecture, light path is also more stablized.The feeler arm of proposition is adopted
Grating is irradiated with different incidence angles with based on two fiber optic collimator mirrors, optical grating diffraction is gone out two groups of spectrum of no overlap outgoing and is believed
Number, the signal of as SECM and OCT, by amasthenic lens, focuses on the left-half of linear charge-coupled array (CCD) respectively
Pixel and right half part pixel, that is, realize detecting the spectral signal from SECM and OCT systems simultaneously.Carried out through incoming computer
Data processing obtains the OCT image of sample axial resolution high and the SECM images of high lateral resolution, realizes OCT systems
Complementary with SECM system advantages cooperates with imaging.This detection method eliminates answering using two spectrometer detection signals simultaneously
Miscellaneous structure, it is only necessary to the spectral signal of SECM systems and OCT systems is detected with a feeler arm so that system architecture is more stepped up
Gather, light path is more stable, while also save build cost, and obtain grating in feeler arm, convex lens and line array CCD
Sufficiently utilize.Additionally, the imaging results of two systems mutually registration, pixel alignment, make the imaging results of system more accurate.This
The optical spectrum encoded confocal above advantage that imaging method and system are cooperateed with optical coherence tomography is planted in biomedical imaging and material
The application fields such as material detection suffer from important meaning.
The above is only the preferred embodiment of the present invention, it should be pointed out that：For the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (3)
- It is 1. a kind of optical spectrum encoded confocal to cooperate with imaging system with optical coherence tomography, it is characterised in that：Including light source (1), reference Arm (2), sample arm (3), feeler arm (4), Transmission Fibers (5), the first optoisolator (6), the second optoisolator (7), the first light Fine coupler (8) and the second fiber coupler (9), one end of first fiber coupler (8) pass through Transmission Fibers (5) respectively Light source (1) and feeler arm (4) are connected respectively, and the other end connects sample arm (3) and the second optical fiber respectively by Transmission Fibers (5) The first optoisolator (6) is provided between one end of coupler (9), and the light source (1) and the first fiber coupler (8), the The second optoisolator (7) is connected between one fiber coupler (8) and the second fiber coupler (9)；Second fiber coupler (9) one end being connected with the first fiber coupler (8) is also connected with feeler arm (4), and second fiber coupler (9) is another One end connects reference arm (2) and sample arm (3) respectively；The sample arm (3) includes the first light for being set gradually by light flow speed and direction Fine collimating mirror (10), the first grating (11), the first convex lens (12), the second convex lens (13) and microcobjective (16), it is described One end of first fiber coupler (8) and the second fiber coupler (9) connection is connected with the first fiber optic collimator mirror (10), and described The spot center of the first grating (11), the first convex lens (12) center, the second convex lens (13) center and microcobjective center On same optical axis；It is Z axis with the first convex lens (12) and the second convex lens (13) optical axis line direction, it is accurate with the second optical fiber The optical axis line direction of straight mirror (14) and galvanometer (15) is Y-axis, and the center with the first convex lens is origin, according to the right-hand rule, Set up coordinate system；First grating (11) surface is perpendicular to YZ planes, and first grating (11) and the angle of XZ are a； Also include the second fiber optic collimator mirror (14) and the galvanometer (15) that are set gradually by beam propagation order, second fiber coupler (9) and reference arm (2) connection one end be connected with the second fiber optic collimator mirror (14), the galvanometer (15) is arranged at the first grating (11) and the first convex lens (12) between, galvanometer (15) surface is accurate with the second optical fiber perpendicular to YZ planes, and galvanometer (15) , in same level, second fiber optic collimator mirror (14) and galvanometer (15) are in the first grating (11) in addition for the height of straight mirror (14) On hot spot lower section along positive X-direction side-play amount>0；First fiber optic collimator mirror (10) will be from the first fiber coupler (8) light beam to be projected and form hot spot on the first grating (11) surface, and it is convex that the spectrum gone out from the first optical grating diffraction is incident upon first The top half of lens (12)；Light beam from the second fiber coupler (9) is incident upon galvanometer by the second fiber optic collimator mirror (14) (15) on, the light beam is reflected in the latter half of the first convex lens (12) by galvanometer (15), by adjusting the first grating (11) The sweep limits of placed angle a and galvanometer (15) causes the scanning angular region and the first grating of the light beam from galvanometer (15) reflection (11) the angle dispersion scope of emergent light is equal；The light beam of two parts up and down of the first convex lens (12) is incident upon by the first convex lens (12) after, the bright line spot of same horizontal direction is focused into the image space focal plane of the first convex lens (12), this bright line spot exists The optical axis perpendicular quadrature of midpoint and the first convex lens (12), and continue to incide on the second convex lens (13)；From the second convex lens The light beam of mirror (13) outgoing is assembled, and all incides microcobjective (16), by the light that microcobjective (16) is focused on Beam is incident upon on sample；The light returned from sample scattering,single is returned and respectively through the first fiber optic collimator mirror through original optical path (10), the second fiber optic collimator mirror (14) returns to respective light path, and enters feeler arm；The feeler arm (4) includes the 3rd optical fiber Collimating mirror (17), the 4th fiber optic collimator mirror (18), the second grating (19), amasthenic lens (20) and linear charge-coupled array (21)；One end of first fiber coupler (8) and light source (1) connection is connected with the 4th fiber optic collimator mirror (18), and described One end of second fiber coupler (9) and the first fiber coupler (8) connection is connected with the 3rd fiber optic collimator mirror (17)；Described Three fiber optic collimator mirrors (17) and the 4th fiber optic collimator mirror (18) are in the same side of the second grating (19), and the 3rd fiber optic collimator Mirror (17) and the 4th fiber optic collimator mirror (18) are placed from the second grating (19) into different angles respectively；And second grating (19) opposite side places linear charge-coupled array (21), and the amasthenic lens (20) is placed on the second grating (19) with linear array electricity Between lotus coupled apparatus (21)；3rd fiber optic collimator mirror (17) collects the light beam for coming from the first fiber coupler (8), and 4th fiber optic collimator mirror (18) collects the light beam for coming from the second fiber coupler (9), and the 3rd fiber optic collimator mirror (17), the 4th fiber optic collimator mirror (18) is respectively projected on the second grating (19) two light beams with different incident angles, is made Second grating (19) diffraction goes out two groups of spectral signals of no overlap outgoing, the signal of as SECM and OCT, by amasthenic lens (20) the left-half pixel and right half part pixel of linear charge-coupled array (21) photosurface, are focused on respectively.
- It is 2. according to claim 1 optical spectrum encoded confocal to cooperate with imaging system with optical coherence tomography, it is characterised in that：Institute It is the focal length sum of two lens to state the distance between the first convex lens (12) and second convex lens (13), the second convex lens (13) with The distance of microcobjective (16) is equal to the focal length of the second convex lens (13).
- It is 3. according to claim 2 optical spectrum encoded confocal to cooperate with imaging system with optical coherence tomography, it is characterised in that：Institute Focus on stating two groups of spectral signal no overlaps on linear charge-coupled array (21) photosurface.
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