CN104224117A - Spectrally encoded confocal and optical coherence tomography cooperative imaging method and system - Google Patents
Spectrally encoded confocal and optical coherence tomography cooperative imaging method and system Download PDFInfo
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Abstract
The invention discloses a spectrally encoded confocal and optical coherence tomography cooperative imaging method and system. The system is based on the combination of an OCT (Optical Coherence Tomography) imaging technique and an SECM (Spectrally Encoded Confocal Microscopy) technique, a sample arm of an OCT system and a sample arm of an SECM system share the same light path, so that light beams emitted from the sample arms of the OCT system and the SECM system simultaneously scan the same position of a sample, and light which is scattered and returns back from the sample at a single time is respectively transmitted to a detection arm through respective light paths. The detection arm adopts two optical fiber collimating lenses to irradiate an optical grating from different incident angles, so that the optical grating diffracts two groups of spectral signals which are emitted without crossing over, i.e. SECM and OCT signals. The light path of the sample arm in the system is simpler, the implementation is facilitated, the light path is more compact and stable, the system imaging quality is high and biological tissue structures can be imaged more accurately.
Description
Technical field
The present invention relates to optical spectrum encoded confocal microscopy and Optical Coherence Tomography Imaging Technology, particularly relate to a kind of optical spectrum encoded confocal technology of working in coordination with synchronous imaging with optical coherence tomography.
Background technology
Optical coherent chromatographic imaging (Optical Coherence Tomography, being called for short OCT) technology is a kind of emerging biomedical optical imaging technique, can realize carrying out noncontact, not damaged, high-resolution imaging to the structure of biological tissue and physiological function, in the earlier detection of disease with have broad application prospects in body biopsy field.
Optical spectrum encoded confocal microscopy (Spectrally Encoded Confocal Microscopy, being called for short SECM) technology is a kind of confocal imaging method of micro-intrusion single-mode fiber, this technology adopts wideband light source and diffraction grating to realize detecting the reflectance at some place, sample multiple lateral attitude simultaneously.By detecting the spatial information of sample arm, pumped FIR laser confocal microscope system can exempt mechanical scanning, provides the vivo biological tissue image that resolution is tending towards electronmicroscopic level, and its compact nature can realize loading in small diameter conduits or endoscopic catheters.
Comparatively speaking, the imaging depth of OCT technology is greater than SECM technology.OCT technology can provide biological tissue's section tomographic map of high axial resolution, SECM technology can provide the biological tissue of high lateral resolution shallow top layer cross-sectional image, the advantage of two kinds of technology in spatial resolution complements one another, therefore the collaborative imaging of OCT technology and SECM technology can provide more high spatial resolution and more comprehensively biological tissue's microscopy information, all will have great importance in the application such as biomedical imaging and non-biological material detection.
In the OCT system proposed at present and the method for SECM systematic collaboration imaging, the switching over realizing between two kinds of systems based on the swing of scanning galvanometer mostly, this employing galvanometer is comparatively complicated as the system structure of mechanical switch, and scanning imagery while not accomplishing truly, if sample is biopsy samples, just probably appear at the situation of two the system imaging position misalignment caused because live body moves in handoff procedure.In addition, in the method for current proposed OCT system and the imaging of SECM systematic collaboration, most employing two light sources are respectively used to OCT system and SECM system, and have employed two feeler arms and detect the sample signal of OCT system and the sample signal of SECM system respectively, make the system after integrating too complicated, stable not, and it is higher to build cost.Therefore, how in the comparatively simple situation of light path, design and a kind of optical spectrum encodedly confocally work in coordination with formation method and system with optical coherence tomography, realize synchronous scanning imaging truly, reduce the quantity of light source and feeler arm simultaneously, make collaborative imaging system structure compacter stable, just become the general objective that OCT system and SECM systematic collaboration imaging system are developed.
Summary of the invention
Goal of the invention: in order to overcome the deficiencies in the prior art, proposes a kind of optical spectrum encodedly confocally to work in coordination with formation method and system with optical coherence tomography.This optical spectrum encoded confocal formation method and system of working in coordination with optical coherence tomography adopts the sample arm of the sample arm of optical coherent chromatographic imaging (OCT) system and optical spectrum encoded confocal microscopy (SECM) system is shared same light path, make synchronously to scan from the light beam of OCT system and SECM system exit sample same position in sample arm, the light returned from sample scattering,single respectively through respective optic path to the fiber optic collimator mirror of two feeler arm, by these two fiber optic collimator mirrors are irradiated grating with different incidence angles, optical grating diffraction is made to go out two groups of spectral signals without overlapping outgoing, be the signal of SECM and OCT, through amasthenic lens, focus on left-half pixel and the right half part pixel of linear charge-coupled array (CCD) respectively, namely realize detecting the spectral signal from SECM and OCT system simultaneously.Carrying out the OCT image of date processing acquisition sample height axial resolution and the SECM image of high lateral resolution through importing computer into, realizing the collaborative imaging of OCT system and the complementation of SECM system advantage.
For achieving the above object, the technical solution used in the present invention is: a kind of optical spectrum encodedly confocally work in coordination with formation method with optical coherence tomography, the sample arm of the sample arm of OCT system and SECM system is shared same light path, make synchronously to scan from the light beam of OCT system and SECM system exit sample same position in sample arm, the light returned from sample scattering,single respectively through respective optic path to the fiber optic collimator mirror of two feeler arm, by these two fiber optic collimator mirrors are irradiated grating with different incidence angles, optical grating diffraction is made to go out two groups of spectral signals without overlapping outgoing, this spectral signal is the signal of SECM and OCT, through amasthenic lens, focus on left-half pixel and the right half part pixel of linear charge-coupled array respectively, namely realize detecting the spectral signal from SECM and OCT system simultaneously.
A kind of optical spectrum encodedly confocally work in coordination with imaging system with optical coherence tomography based on according to claim 1, comprise light source (1), reference arm (2), sample arm (3), feeler arm (4), Transmission Fibers (5), first optoisolator (6), second optoisolator (7), first fiber coupler (8) and the second fiber coupler (9), one end of described first fiber coupler (8) connects light source (1) and feeler arm (4) respectively respectively by Transmission Fibers (5), and the other end connects one end of sample arm (3) and the second fiber coupler (9) respectively by Transmission Fibers (5), and be provided with the first optoisolator (6) between described light source (1) and the first fiber coupler (8), the second optoisolator (7) is connected between first fiber coupler (8) and the second fiber coupler (9), one end that described second fiber coupler (9) is connected with the first fiber coupler (8) is also connected with feeler arm (4), and the other end of described second fiber coupler (9) connects reference arm (2) and sample arm (3) respectively.
Described sample arm (3) comprises and flows to by the light velocity the first fiber optic collimator mirror (10), the first grating (11), the first convex lens (12), the second convex lens (13) and the microcobjective (16) that set gradually, one end that described first fiber coupler (8) is connected with the second fiber coupler (9) is connected with the first fiber optic collimator mirror (10), and the spot center of described first grating (11), the first convex lens (12) center, the second convex lens (13) center and microcobjective center are on same optical axis, with the first convex lens (12) and the second convex lens (13) optical axis line direction for Z axis, with the optical axis line direction of the second fiber optic collimator mirror (14) and galvanometer (15) for Y-axis, with the center of the first convex lens for initial point, according to the right-hand rule, set up coordinate system, described first grating (11) surface is perpendicular to YZ plane, and described first grating (11) is a with the angle of XZ, also comprise the second fiber optic collimator mirror (14) and galvanometer (15) that set gradually by beam propagation order, one end that described second fiber coupler (9) is connected with reference arm (2) is connected with the second fiber optic collimator mirror (14), described galvanometer (15) is arranged between the first grating (11) and the first convex lens (12), described galvanometer (15) surface is perpendicular to YZ plane, and the height of galvanometer (15) and the second fiber optic collimator mirror (14) is in same level, in addition below described second fiber optic collimator mirror (14) and the hot spot of galvanometer (15) on the first grating (11) along the side-play amount >0 of positive X-direction, beam projection from the first fiber coupler (8) is formed hot spot to the first grating (11) by described first fiber optic collimator mirror (10) on the surface, and the spectrum gone out from the first optical grating diffraction is incident upon the first half of the first convex lens (12), second fiber optic collimator mirror (14) will from the beam projection of the second fiber coupler (9) on galvanometer (15), this light beam is reflected in the latter half of the first convex lens (12) through galvanometer (15), is made the scan angle scope of the light beam reflected from galvanometer (15) equal with the angle dispersion scope of the first grating (11) emergent light by the sweep limits adjusting the first grating (11) placed angle a and galvanometer (15), be incident upon the light beam of two parts up and down of the first convex lens (12) after the first convex lens (12), the bright line speckle of same horizontal direction is focused in the image space focal plane of the first convex lens (12), this bright line speckle at the optical axis perpendicular quadrature of midpoint and the first convex lens (12), and continues to incide on the second convex lens (13), assembled from the light beam of the second convex lens (13) outgoing, and all incide in microcobjective (16), the beam projection focused on through microcobjective (16) is on sample, the light returned from sample scattering,single returns through original optical path and returns respective light path respectively through the first fiber optic collimator mirror (10), the second fiber optic collimator mirror (14), and enter feeler arm.
Described feeler arm (4) comprises the 3rd fiber optic collimator mirror (17), the 4th fiber optic collimator mirror (18), the second grating (19), amasthenic lens (20) and linear charge-coupled array (21), one end that described first fiber coupler (8) is connected with light source (1) is connected with the 4th fiber optic collimator mirror (18), and one end that described second fiber coupler (9) is connected with the first fiber coupler (8) is connected with the 3rd fiber optic collimator mirror (17), described 3rd fiber optic collimator mirror (17) and the 4th fiber optic collimator mirror (18) are in the same side of the second grating (19), and described 3rd fiber optic collimator mirror (17) and the 4th fiber optic collimator mirror (18) become different angles to place respectively from the second grating (19), and described second grating (19) opposite side places linear charge-coupled array (21), described amasthenic lens (20) is placed between the second grating (19) and linear charge-coupled array (21), the light beam coming from the first fiber coupler (8) collected by described 3rd fiber optic collimator mirror (17), and the light beam coming from the second fiber coupler (9) collected by described 4th fiber optic collimator mirror (18), and described 3rd fiber optic collimator mirror (17), 4th fiber optic collimator mirror (18) respectively with different incident angles by two beam projection on the second grating (19), the second grating (19) diffraction is made to go out two groups of spectral signals without overlapping outgoing, be the signal of SECM and OCT, through amasthenic lens (20), focus on left-half pixel and the right half part pixel of line array CCD (21) photosurface respectively.
Preferred: the distance between described first convex lens (12) and the second convex lens (13) is the focal length sum of two lens, the second convex lens (13) equals the focal length of the second convex lens (13) with the distance of microcobjective (16).Described two groups of spectral signals focus on CCD photosurface without overlapping.
One provided by the invention is optical spectrum encoded confocally works in coordination with formation method and system with optical coherence tomography, compared to existing technology, has following beneficial effect:
1, by adopting, the sample arm of the sample arm of optical coherence tomography system and optical spectrum encoded confocal microscope system is shared same light path, make synchronously to scan from the light beam of OCT system and SECM system exit sample same position in sample arm, effectively overcome by two systems in conjunction with time use the problem brought of mechanical switch, really accomplished synchronous scanning sample.In addition, the method and system before comparing, the light path of the sample arm in the present invention is more simple, is convenient to implement, and light path is also compacter, stable.
2, optical spectrum encodedly confocally formation method and system is worked in coordination with optical coherence tomography by adopting, the axial resolution of this system is made to have had large increase relative to independent SECM system, and the lateral resolution of this system is greatly improved relative to independent OCT system, greatly optimizes the image quality of system.
3, by adopting, the sample arm of the sample arm of optical coherence tomography system and optical spectrum encoded confocal microscope system being shared same light path, only needing employing light source; In addition, by adopting light path optical coherence tomography system and optical spectrum encoded confocal microscope system being realized detecting in feeler arm simultaneously, OCT system and SECM system is made to realize detection imaging simultaneously, this detection method eliminates and uses two feeler arms to carry out the trouble of detectable signal simultaneously, only need the spectral signal simultaneously detecting OCT system and SECM system with a feeler arm, make system structure compacter, light path is more stable, and has saved and build cost.
4, optical spectrum encodedly confocally working in coordination with formation method and system with optical coherence tomography by adopting, the mutual registration of the imaging results of SECM system and OCT system, pixel being alignd, thus makes this system more accurate to mechanics of biological tissue imaging.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention;
Fig. 2 is the schematic diagram of sample arm;
Fig. 3 is the schematic diagram of feeler arm;
In figure: 1, light source, 2, reference arm, 3, sample arm, 4, feeler arm, 5, Transmission Fibers, the 6, first optoisolator, the 7, second optoisolator, 8, the first fiber coupler, 9, the second fiber coupler, the 10, first fiber optic collimator mirror, the 11, first grating, 12, the first convex lens, 13, the second convex lens, the 14, second fiber optic collimator mirror, 15, galvanometer, 16, microcobjective, 17, the 3rd fiber optic collimator mirror, the 18, the 4th fiber optic collimator mirror, the 19, second grating, 20, amasthenic lens, 21, linear charge-coupled array (CCD).
Detailed description of the invention
Below in conjunction with drawings and Examples, the present invention is further illustrated, and object of the present invention and effect will become more obvious.
A kind of optical spectrum encodedly confocally work in coordination with formation method with optical coherence tomography, as Figure 1-3, the sample arm of the sample arm of OCT system and SECM system is shared same light path, make synchronously to scan from the light beam of OCT system and SECM system exit sample same position in sample arm, the light returned from sample scattering,single respectively through respective optic path to the fiber optic collimator mirror of two feeler arm, by these two fiber optic collimator mirrors are irradiated grating with different incidence angles, optical grating diffraction is made to go out two groups of spectral signals without overlapping outgoing, this spectral signal is the signal of SECM and OCT, through amasthenic lens, focus on left-half pixel and the right half part pixel of linear charge-coupled array respectively, namely realize detecting the spectral signal from SECM and OCT system simultaneously.
A kind of optical spectrum encodedly confocally work in coordination with imaging system with optical coherence tomography based on according to claim 1, as shown in Figure 1, comprise light source 1, reference arm 2, sample arm 3, feeler arm 4, Transmission Fibers 5, first optoisolator 6, second optoisolator 7, first fiber coupler 8 and the second fiber coupler 9, one end of described first fiber coupler 8 connects light source 1 and feeler arm 4 respectively respectively by Transmission Fibers 5, and the other end connects one end of sample arm 3 and the second fiber coupler 9 respectively by Transmission Fibers 5, and be provided with the first optoisolator 6 between described light source 1 and the first fiber coupler 8, the second optoisolator 7 is connected between first fiber coupler 8 and the second fiber coupler 9, one end that described second fiber coupler 9 is connected with the first fiber coupler 8 is also connected with feeler arm 4, and the other end of described second fiber coupler 9 connects reference arm 2 and sample arm 3 respectively.
The broadband light that light source 1 sends transfers to the first fiber coupler 8 through Transmission Fibers 5 and the first optoisolator 6, and is divided into two-beam and transfers to the first fiber optic collimator mirror 10 and the second optoisolator 7 in sample arm 3 respectively.Reflect through sample arm 3 from the light beam of the first fiber optic collimator mirror 10 incidence, again transfer to the 4th collimating mirror 18 in feeler arm 4 through the first fiber coupler 8 and Transmission Fibers 5 and detect.Two parts are divided into through the second fiber coupler 9 from the light beam of the second optoisolator 7 outgoing, part light beam enters reference arm 2 and reflects, another part light beam enters the second fiber optic collimator mirror 14 of sample arm 3 and reflecting through sample arm 3, the two-beam reflected is coupled through the second fiber coupler 9 and interferes, and the three fiber optic collimator mirror 17 of interference light in the incident feeler arm 4 of Transmission Fibers 5 entering in feeler arm detects.
Reference arm 2 is for providing reference optical signal.Typical reference arm is made up of fiber optic collimator mirror, convex lens and the reflecting mirror be fixed on translation stage.
Optoisolator is a kind of magneto-optic device that can absorb or offset reverse transfers.Fibre optic isolater is generally used for protection light source from the impact of back reflection, prevents the optical damage that intensity noise causes.The optoisolator that typical optoisolator parameter can be produced with reference to the Thorlabs company of the U.S..
Fiber coupler is a kind of element for realizing optical signal shunt or conjunction road.The fiber coupler that typical optoisolator parameter can be produced with reference to the Thorlabs company of the U.S..
As shown in Figure 2, described sample arm 3 comprises and flows to by the light velocity the first fiber optic collimator mirror 10, first grating 11, first convex lens 12, second convex lens 13 and the microcobjective 16 set gradually, one end that described first fiber coupler 8 is connected with the second fiber coupler 9 is connected with the first fiber optic collimator mirror 10, and the spot center of described first grating 11, the first convex lens 12 center, the second convex lens 13 center and microcobjective center are on same optical axis; With the first convex lens 12 and the second convex lens 13 optical axis line direction for Z axis, with the optical axis line direction of the second fiber optic collimator mirror 14 and galvanometer 15 for Y-axis, namely as shown in Figure 2, vertical paper direction and direction perpendicular to the first convex lens 12 and second convex lens 13 line of centres is Y-axis, with the center of the first convex lens for initial point, according to the right-hand rule, set up coordinate system; Described first grating 11 surface is perpendicular to YZ plane, and described first grating 11 is a with the angle of XZ; Also comprise the second fiber optic collimator mirror 14 and galvanometer 15 that set gradually by beam propagation order, one end that described second fiber coupler 9 is connected with reference arm 2 is connected with the second fiber optic collimator mirror 14, described galvanometer 15 is arranged between the first grating 11 and the first convex lens 12, described galvanometer 15 surface is perpendicular to YZ plane, and the height of galvanometer 15 and the second fiber optic collimator mirror 14 is in same level, in addition below described second fiber optic collimator mirror 14 and the hot spot of galvanometer 15 on the first grating 11 along the side-play amount >0 of positive X-direction;
Beam projection from the first fiber coupler 8 is formed hot spot to the first grating 11 by described first fiber optic collimator mirror 10 on the surface, and the spectrum gone out from the first optical grating diffraction is incident upon the first half of the first convex lens 12; Second fiber optic collimator mirror 14 by the beam projection from the second fiber coupler 9 on galvanometer 15, this light beam is reflected in the latter half of the first convex lens 12 through galvanometer 15, is made the scan angle scope of the light beam reflected from galvanometer 15 equal with the angle dispersion scope of the first grating 11 emergent light by the sweep limits adjusting the first grating 11 placed angle a and galvanometer 15.Be incident upon the light beam of two parts up and down of the first convex lens 12 after the first convex lens 12, the bright line speckle of same horizontal direction is focused in the image space focal plane of the first convex lens 12, this bright line speckle at the optical axis perpendicular quadrature of midpoint and the first convex lens 12, and continues to incide on the second convex lens 13; Assembled from the light beam of the second convex lens 13 outgoing, and all incided in microcobjective 16, through microcobjective 16 focus on beam projection on sample; The light returned from sample scattering,single returns through original optical path and returns respective light path respectively through the first fiber optic collimator mirror 10, second fiber optic collimator mirror 14, and enters feeler arm.Distance between described first convex lens 12 and the second convex lens 13 is the focal length sum of two lens, and the second convex lens 13 and the distance of microcobjective 16 equal the focal length of the second convex lens 13.Described two groups of spectral signals focus on CCD photosurface without overlapping.
Concrete: the first fiber optic collimator mirror 10 is collected the light beam from optical spectrum encoded confocal microscopy SECM system and is projected on the first grating 11, the spectrum gone out from the first optical grating diffraction is incident upon the first half of the first convex lens 12, second fiber optic collimator mirror 14 is collected the light beam from optical coherent chromatographic imaging OCT system and is incident upon the latter half of the first convex lens 12 by the reflection of galvanometer 15, made the scan angle scope of the light beam reflected from galvanometer 15 equal with the angle dispersion scope of the first grating 11 emergent light by the sweep limits adjusting the first grating 11 placed angle and galvanometer 15.Be incident upon the light beam of two parts up and down of the first convex lens 12 after the first convex lens 12, the bright line of a horizontal direction is focused in the image space focal plane of the first convex lens 12, this bright line is at the optical axis perpendicular quadrature of midpoint and the first convex lens 12, two-beam is coupled since then, and continues to incide in the second convex lens 13.Restrained when the light beam of the second convex lens 13 outgoing compares incident first convex lens 12, and all incided in microcobjective 16, through microcobjective 16 focus on beam projection on sample.The light returned from sample scattering,single returns through original optical path and returns respective light path respectively through the first fiber optic collimator mirror 10, second fiber optic collimator mirror 14, and enters feeler arm.
Grating can make light beam generation dispersion, the light of different wave length is beaten on sample successively, thus realizes optical spectrum encoded.Grating can be divided into transmissive diffraction grating and reflective diffraction gratings, the grating that typical grating parameter can be produced with reference to the Wasatch Photonics company of the U.S..
Fiber optic collimator mirror is accurately located by tail optical fiber and GRIN Lens and is formed, and the diverging light that optical fiber sends can be transformed into the parallel spatial light of collimation by it.
The drive singal that galvanometer 15 can send according to computer controller, makes its optical scanning head realize swaying, thus makes the light beam reflected from this optical scanning head a plane interscan.Be usually used in the sample arm of OCT system, realizing the scanning of light beam to sample diverse location place.
The beam heights of sample arm can focus on by microcobjective 16, makes the luminous point got on sample less, thus the lateral resolution of system is improved.
As shown in Figure 3, described feeler arm 4 comprises the 3rd fiber optic collimator mirror 17, the 4th fiber optic collimator mirror 18, second grating 19, amasthenic lens 20 and linear charge-coupled array 21; One end that described first fiber coupler 8 is connected with light source 1 is connected with the 4th fiber optic collimator mirror 18, and one end that described second fiber coupler 9 is connected with the first fiber coupler 8 is connected with the 3rd fiber optic collimator mirror 17; Described 3rd fiber optic collimator mirror 17 and the 4th fiber optic collimator mirror 18 are in the same side of the second grating 19, and described 3rd fiber optic collimator mirror 17 angle different from 19 one-tenth, the second grating respectively with the 4th fiber optic collimator mirror 18 is placed; And described second grating 19 opposite side places linear charge-coupled array 21, described amasthenic lens 20 is placed between the second grating 19 and linear charge-coupled array 21.
The light beam coming from the first fiber coupler 8 collected by described 3rd fiber optic collimator mirror 17, and the light beam coming from the second fiber coupler 9 collected by described 4th fiber optic collimator mirror 18, and described 3rd fiber optic collimator mirror 17, the 4th fiber optic collimator mirror 18 respectively with different incident angles by two beam projection on the second grating 19, the second grating 19 diffraction is made to go out two groups of spectral signals without overlapping outgoing, be the signal of SECM and OCT, through amasthenic lens 20, focus on left-half pixel and the right half part pixel of line array CCD 21 photosurface respectively.
Concrete: the 3rd fiber optic collimator mirror 17, the light beam of OCT system and SECM system collected respectively by 4th fiber optic collimator mirror 18, and with different incident angles by the beam projection from two systems on the second grating 19, by calculating and adjust the size of these two angle of incidence, the second grating 19 diffraction is made to go out two groups of spectral signals without overlapping outgoing, be the signal of SECM and OCT, and the angle of emergence that wherein maximum wavelength of one group of spectral signal is corresponding is equal with another angle of emergence organizing the minimum wavelength of spectral signal corresponding, these two groups of spectral signals are propagated respectively in the space of the right and left like this, and two groups of spectral signals are tightly adjacent, project subsequently in amasthenic lens 20.Through the focusing of amasthenic lens 20, make these two groups of spectral signals on the photosurface of linear array CCD21, be focused into a bright line, left and right one side of something of this bright line is respectively the spectral signal of SECM system and OCT system, and namely the left-half of line array CCD 21 photosurface and right half part detect the spectral signal of SECM system and OCT system respectively.
Line array CCD 21 can be real-time light intensity signal is converted into voltage signal, voltage signal is transferred to computer collection through data transmission cable.The line array CCD (Aviiva SM2) that typical line array CCD parameter can be produced with reference to French Atmel company.Compared with traditional picture pick-up device, line array CCD 21 has that spectral response is wide, good linearity, wide dynamic range, noise are low, highly sensitive, many-sided advantage such as real-time Transmission and electric charge self-scanning, has been widely used in the fields such as remotely sensed image, satellite monitoring, machine vision at present.
One disclosed by the invention is optical spectrum encoded confocally works in coordination with formation method and system with optical coherence tomography, what propose shares same light path by collaborative for the optical coherence tomography sample arm of (OCT) imaging system and the sample arm of optical spectrum encoded confocal (SECM) system, make synchronously to scan from the light beam of OCT system and SECM system exit sample same position in sample arm, and return respective light path respectively from the light that sample scattering,single returns and return feeler arm, the method of this optical coupled effectively overcome by two systems in conjunction with time use mechanical switch to bring problem, really accomplish the same position of synchronous scanning sample.Thisly optical spectrum encodedly confocally work in coordination with formation method and system with optical coherence tomography, the axial resolution of this system is made to have had large increase relative to SECM system, and its lateral resolution is greatly improved relative to OCT system, optimizes the image quality of system.In addition, the method and system before comparing, the light path of the sample arm in the present invention is more simple, and be convenient to implement, and only need employing light source, saved cost and simplified system structure, light path is also more stable.The feeler arm proposed adopts and irradiates grating based on two fiber optic collimator mirrors with different incidence angles, optical grating diffraction is made to go out two groups of spectral signals without overlapping outgoing, be the signal of SECM and OCT, through amasthenic lens, focus on left-half pixel and the right half part pixel of linear charge-coupled array (CCD) respectively, namely realize detecting the spectral signal from SECM and OCT system simultaneously.Carrying out the OCT image of date processing acquisition sample height axial resolution and the SECM image of high lateral resolution through importing computer into, realizing the collaborative imaging of OCT system and the complementation of SECM system advantage.This detection method eliminates the labyrinth simultaneously using two spectrometer detection signals, only need the spectral signal by a feeler arm detection SECM system and OCT system, make system structure compacter, light path is more stable, also save simultaneously and build cost, and the grating in feeler arm, convex lens and line array CCD are fully utilized.In addition, the mutual registration of imaging results of two systems, pixel alignment, make the imaging results of system more accurate.This optical spectrum encoded confocal above advantage of working in coordination with formation method and system with optical coherence tomography all has great significance in the application such as biomedical imaging and material tests.
The above is only the preferred embodiment of the present invention; be noted that for those skilled in the art; under the premise without departing from the principles of the invention, can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (6)
1. optical spectrum encodedly confocally work in coordination with formation method with optical coherence tomography for one kind, it is characterized in that: the sample arm of the sample arm of OCT system and SECM system is shared same light path, make synchronously to scan from the light beam of OCT system and SECM system exit sample same position in sample arm, the light returned from sample scattering,single respectively through respective optic path to the fiber optic collimator mirror of two feeler arm, by these two fiber optic collimator mirrors are irradiated grating with different incidence angles, optical grating diffraction is made to go out two groups of spectral signals without overlapping outgoing, this spectral signal is the signal of SECM and OCT, through amasthenic lens, focus on left-half pixel and the right half part pixel of linear charge-coupled array respectively, namely realize detecting the spectral signal from SECM and OCT system simultaneously.
2. optical spectrum encodedly confocally work in coordination with imaging system with optical coherence tomography based on according to claim 1 for one kind, it is characterized in that: comprise light source (1), reference arm (2), sample arm (3), feeler arm (4), Transmission Fibers (5), first optoisolator (6), second optoisolator (7), first fiber coupler (8) and the second fiber coupler (9), one end of described first fiber coupler (8) connects light source (1) and feeler arm (4) respectively respectively by Transmission Fibers (5), and the other end connects one end of sample arm (3) and the second fiber coupler (9) respectively by Transmission Fibers (5), and be provided with the first optoisolator (6) between described light source (1) and the first fiber coupler (8), the second optoisolator (7) is connected between first fiber coupler (8) and the second fiber coupler (9), one end that described second fiber coupler (9) is connected with the first fiber coupler (8) is also connected with feeler arm (4), and the other end of described second fiber coupler (9) connects reference arm (2) and sample arm (3) respectively.
3. according to claim 2ly optical spectrum encodedly confocally work in coordination with imaging system with optical coherence tomography, it is characterized in that: described sample arm (3) comprises and flows to by the light velocity the first fiber optic collimator mirror (10) set gradually, first grating (11), first convex lens (12), second convex lens (13) and microcobjective (16), one end that described first fiber coupler (8) is connected with the second fiber coupler (9) is connected with the first fiber optic collimator mirror (10), and the spot center of described first grating (11), first convex lens (12) center, second convex lens (13) center and microcobjective center are on same optical axis, with the first convex lens (12) and the second convex lens (13) optical axis line direction for Z axis, with the optical axis line direction of the second fiber optic collimator mirror (14) and galvanometer (15) for Y-axis, with the center of the first convex lens for initial point, according to the right-hand rule, set up coordinate system, described first grating (11) surface is perpendicular to YZ plane, and described first grating (11) is a with the angle of XZ, also comprise the second fiber optic collimator mirror (14) and galvanometer (15) that set gradually by beam propagation order, one end that described second fiber coupler (9) is connected with reference arm (2) is connected with the second fiber optic collimator mirror (14), described galvanometer (15) is arranged between the first grating (11) and the first convex lens (12), described galvanometer (15) surface is perpendicular to YZ plane, and the height of galvanometer (15) and the second fiber optic collimator mirror (14) is in same level, in addition below described second fiber optic collimator mirror (14) and the hot spot of galvanometer (15) on the first grating (11) along the side-play amount >0 of positive X-direction, beam projection from the first fiber coupler (8) is formed hot spot to the first grating (11) by described first fiber optic collimator mirror (10) on the surface, and the spectrum gone out from the first optical grating diffraction is incident upon the first half of the first convex lens (12), second fiber optic collimator mirror (14) will from the beam projection of the second fiber coupler (9) on galvanometer (15), this light beam is reflected in the latter half of the first convex lens (12) through galvanometer (15), is made the scan angle scope of the light beam reflected from galvanometer (15) equal with the angle dispersion scope of the first grating (11) emergent light by the sweep limits adjusting the first grating (11) placed angle a and galvanometer (15), be incident upon the light beam of two parts up and down of the first convex lens (12) after the first convex lens (12), the bright line speckle of same horizontal direction is focused in the image space focal plane of the first convex lens (12), this bright line speckle at the optical axis perpendicular quadrature of midpoint and the first convex lens (12), and continues to incide on the second convex lens (13), assembled from the light beam of the second convex lens (13) outgoing, and all incide in microcobjective (16), the beam projection focused on through microcobjective (16) is on sample, the light returned from sample scattering,single returns through original optical path and returns respective light path respectively through the first fiber optic collimator mirror (10), the second fiber optic collimator mirror (14), and enter feeler arm.
4. according to claim 3ly optical spectrum encodedly confocally work in coordination with imaging system with optical coherence tomography, it is characterized in that: described feeler arm (4) comprises the 3rd fiber optic collimator mirror (17), the 4th fiber optic collimator mirror (18), the second grating (19), amasthenic lens (20) and linear charge-coupled array (21), one end that described first fiber coupler (8) is connected with light source (1) is connected with the 4th fiber optic collimator mirror (18), and one end that described second fiber coupler (9) is connected with the first fiber coupler (8) is connected with the 3rd fiber optic collimator mirror (17), described 3rd fiber optic collimator mirror (17) and the 4th fiber optic collimator mirror (18) are in the same side of the second grating (19), and described 3rd fiber optic collimator mirror (17) and the 4th fiber optic collimator mirror (18) become different angles to place respectively from the second grating (19), and described second grating (19) opposite side places linear charge-coupled array (21), described amasthenic lens (20) is placed between the second grating (19) and linear charge-coupled array (21), the light beam coming from the first fiber coupler (8) collected by described 3rd fiber optic collimator mirror (17), and the light beam coming from the second fiber coupler (9) collected by described 4th fiber optic collimator mirror (18), and described 3rd fiber optic collimator mirror (17), 4th fiber optic collimator mirror (18) respectively with different incident angles by two beam projection on the second grating (19), the second grating (19) diffraction is made to go out two groups of spectral signals without overlapping outgoing, be the signal of SECM and OCT, through amasthenic lens (20), focus on left-half pixel and the right half part pixel of line array CCD (21) photosurface respectively.
5. according to claim 4ly optical spectrum encodedly confocally work in coordination with imaging system with optical coherence tomography, it is characterized in that: the distance between described first convex lens (12) and the second convex lens (13) is the focal length sum of two lens, the second convex lens (13) equals the focal length of the second convex lens (13) with the distance of microcobjective (16).
6. according to claim 5ly optical spectrum encodedly confocally work in coordination with imaging system with optical coherence tomography, it is characterized in that: described two groups of spectral signals focus on CCD photosurface without overlapping.
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