CN104062293A - Method for detecting advance yeast coherence causing factors in barley or malt - Google Patents
Method for detecting advance yeast coherence causing factors in barley or malt Download PDFInfo
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- CN104062293A CN104062293A CN201410314223.5A CN201410314223A CN104062293A CN 104062293 A CN104062293 A CN 104062293A CN 201410314223 A CN201410314223 A CN 201410314223A CN 104062293 A CN104062293 A CN 104062293A
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- fructus hordei
- hordei germinatus
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Abstract
The invention relates to the field of beer brewing, in particular to a method for detecting advance yeast coherence causing factors in barley or malt. According to the method, yeast does not need to be used, and the PYF vitality in the barley and the malt can be directly reflected by determining the content of water-soluble xylan and directly detected; compared with an existing fermentation method and an optical density colorimetric method, the yeast is not used, influence of the objective condition like the yeast activity can be avoided, and the method has the advantages of being high in data accuracy and good in repeatability.
Description
Technical field
The present invention relates to brewing field, it is particularly related to a kind of detection and in barley or Fructus Hordei Germinatus, causes the method that yeast shifts to an earlier date the coherency factor.
Background technology
The yeast Yeast Flocculation phenomenon that (premature yeast flocculation, PYF) refers to that yeast ferments during the fermentation while not yet finishing and to occur of flocculating in advance.The PYF factor is a negative barley, malt quality index, derives from barley and Fructus Hordei Germinatus, and its stable chemical nature is heat-resisting, once enter brewing process, is difficult to dispel its negative effect, is the factor that affects latter stage of ripening yeast number most critical.
At present, in Fructus Hordei Germinatus, to shift to an earlier date the detection major part of AgF be to be based upon in yeast-leavened mechanism to yeast, and these methods are totally divided into two classes from principle, and a class is rapid fermentation method, and ultimate principle is: Fructus Hordei Germinatus PYF factor pair yeast flocculence exerts an influence; Use active good production yeast to be added in the agreement act wheat juice of improvement, the suspension yeast count after fermenting by detection, determines the impact of Fructus Hordei Germinatus on yeast flocculence; Another kind of is optical density colourimetry, and the PYF factor of reaching an agreement in wheat juice by extraction makes it react in specific reaction system with yeast, by the turbidity value of supernatant after assaying reaction, and the PYF factor of reflection Fructus Hordei Germinatus.The common point of these two kinds of methods all needs to use yeast, what reflect is Fructus Hordei Germinatus PYF vigor, the combined influence of inoculation yeast state to Yeast Flocculation performance, be applicable to the flocculation performance for predict inoculation yeast under formula Fructus Hordei Germinatus large production latter stage of ripening, be not suitable for for objective evaluation Fructus Hordei Germinatus PYF quality and instruct malthouse's wheat process optimization processed.
This detection method, provide a kind of can visual inspection barley and Fructus Hordei Germinatus PYF vigor (not needing to utilize yeast), accurately, the PYF factors check method of favorable reproducibility, the method is not subject to the impact of the objective condition such as yeast activity, oxygenation, compare with fermentation method, data accurately, favorable reproducibility, time short (shortening to 9 hours by 40 hours).
Summary of the invention
The present invention is directed to the deficiencies in the prior art, the object of the invention is to develop a kind of do not need to use yeast can visual inspection barley and Fructus Hordei Germinatus PYF vigor, accurately, the detection method of favorable reproducibility, with its prediction barley and Fructus Hordei Germinatus, cause the ability that yeast flocculates in advance.
The PYF factor is because barley ectoderm is subject to microbial infection, the xylan fragment a kind of water-soluble character, that there is certain molecular weight producing, therefore can extract, be purified into the xylan fragment that this part can characterize barley, Fructus Hordei Germinatus PYF vigor, by xylan content, directly reflect the PYF vigor of barley, Fructus Hordei Germinatus.
Technical scheme of the present invention is:
Detect in barley or Fructus Hordei Germinatus and cause the method that yeast shifts to an earlier date the coherency factor (PYF), the step of described method is as follows:
Step 1: get the sample that moisture is Wc, pulverize, weigh sample W
2for 25g is in the triangular flask of 500mL, add 300mL deionized water, mix; The 150rpm 1-2h that vibrates, adds water constant weight to W
1for 350g, Filter paper filtering, filtered fluid detects proportion S
g, extract Me; Get filtered fluid 10mL and add 30mL absolute ethyl alcohol, mix; Ice bath alcohol precipitation reaction 1h; The centrifugal 10min of sample 10000rpm after reaction, removes supernatant, and precipitation adds 10mL deionized water to mix, and 85 ℃ of water-bath 2h dissolve; After dissolving, 10000rpm carries out centrifugal removal precipitation, and the freezing preservation of supernatant is standby;
Step 2: drawing standard curve, utilize wood sugar and the phloroglucin chromogenic reaction under acid condition, form red material, measure content, computing formula is as follows:
Y＝a X
552+b
Wherein: X
552for 552nm up-to-date style product absorbance; A is the slope of typical curve; B is the intercept of typical curve; Y is xylose concentration in supernatant, mg/L;
Wherein: G is Water soluble pentosan content in sample, mg/100g over dry Fructus Hordei Germinatus; N is extension rate; Y is xylose concentration in supernatant, mg/L; Wc is the moisture of sample, %; Me is the extract content of brewer's wort, g; S
gproportion for brewer's wort; W
1for sample wheat juice weight, g; W
2for example weight, g; 0.65 is wood sugar and xylan conversion coefficient.
Preferably, the sample described in step 1 is barley or Fructus Hordei Germinatus.
Preferably, in step 1, in ice bath alcohol precipitation course of reaction, within every 20 minutes, shake up once.
Preferably, in step 1, sample is pulverized and is adopted EBC comminutor.
Preferably, in step 2, wood sugar Specification Curve of Increasing method is: the configuration of 500mg/L wood sugar standard solution: 50mg wood sugar is settled to 100mL with deionized water; Take out respectively 1.0,2.0,3.0,4.0,6.0, wood sugar standard solution described in 8.0mL, be diluted to 10mL, the wood sugar standard solution of preparation variable concentrations, get respectively the standard solution 1mL after dilution, add after phloroglucin-glacial acetic acid reactant liquor 5mL, mix boiling water bath reaction 25min, ice bath cessation reaction; The water of take adds reactant liquor as contrast, measures each concentration wood sugar at 552nm absorbance, according to known xylose concentration value, Criterion curve.
Preferably, the step of step 2 chromogenic reaction is: get 3 color-comparison tubes and identify respectively, number; Blank: in No. 1 color comparison tube, to add 1mL distilled water; Sample: add 1mL to prepare sample in 2, No. 3 color comparison tubes; In color comparison tube, respectively add phloroglucin-glacial acetic acid reactant liquor 5mL; Immediately 3 color comparison tubes are put into boiling water bath reaction 25 minutes, at stage of reaction color comparison tube, needed stopper beyond the Great Wall; Ice bath cessation reaction; With blank, regulate instrument zero, under 552nm wavelength, detect the light absorption value of testing sample.
At present a lot of to the research of the Fructus Hordei Germinatus PYF factor, about its composition and molecular structure, also not come to a conclusion, the result that different research institutions provides is all not identical.At this, we are not that specifically to study the PYF factor be what on earth, and its molecular structure how, just hopes and extract the material that can cause Yeast Flocculation in barley or Fructus Hordei Germinatus, represents the number of the PYF factor with the xylan content in this material.
Compared with prior art, the present invention has following features:
One: the present invention is with respect to current Rapid Fermentation detection method, and shorten detection time, shortened to 9 hours by original 40 hours;
Its two: the present invention, with respect to existing fermentation method and optical density colourimetry, does not use yeast, is not subject to the impact of the objective condition such as yeast activity, has the data precision high, the advantage of favorable reproducibility;
Its three: the present invention has set up the relation of Water soluble pentosan and PYF, defines it and can substitute PYF and detect.
Therefore, the present invention can detect the PYF factor in barley, Fructus Hordei Germinatus, is the incoming inspection of Brewery Fructus Hordei Germinatus, and the daily quality management of Fructus Hordei Germinatus factory provides a kind of effective method.
Accompanying drawing explanation
Accompanying drawing 1 is Water soluble pentosan and PYF related coefficient.
Embodiment
The specific embodiment of the present invention is as follows:
Embodiment 1:
Get 12 Fructus Hordei Germinatus samples of different manufacturers, Fructus Hordei Germinatus kind comprises and adds wheat, Australia wheat, state wheat, with 20 ℃, 40 hours fermentation (rapid fermentation method) measure respectively the PYF of Fructus Hordei Germinatus, record the PYF of sample from 20%～159%.
One, Water soluble pentosan detects
By each accurate weighing 25.0g of these 12 samples, add 300mL water, shake after 1 hour on the shaking table of 150rpm, balance constant weight is to 350.0g, with Medium speed filter paper, filter, get 10mL filtrate and add 30mL absolute ethyl alcohol, in ice bath, alcohol precipitation is 1 hour, and centre shakes up (every 20 minutes once) twice, the centrifugal 10min of sample 10000rpm after reaction, remove supernatant, precipitation adds 10mL deionized water to mix, and 85 ℃ of water-bath 2h dissolve.After dissolving, 10000rpm carries out centrifugal removal precipitation, gets supernatant standby.
Phloroglucin-glacial acetic acid reactant liquor preparation (developer): take 1.28g phloroglucin and be dissolved in 5mL absolute ethyl alcohol, use 55mL glacial acetic acid, 1mL concentrated hydrochloric acid, 5mL phloroglucin-ethanolic solution, the D/W of 0.5mL1.75% mixes.
Get 3 color comparison tubes and identify respectively, number, in 1# color comparison tube, add 1mL distilled water, 2#, 3# color comparison tube add respectively 1mL to prepare sample, in 3 color comparison tubes, respectively add 5mL phloroglucin-glacial acetic acid reactant liquor, in boiling water bath, react 25 minutes, ice bath cessation reaction, regulates instrument zero with blank, under 552nm wavelength, detect the light absorption value of testing sample, the Water soluble pentosan content (mg/100g over dry Fructus Hordei Germinatus) that calculates sample according to formula is as table 1:
Table 1:
Two, traditional zymotic method detects Fructus Hordei Germinatus PYF
Use saccharifying instrument to prepare agreement act wheat juice in 12 samples, get filtrate 280mL in SCOTT bottle, build bottle cap, put into pot, after boiling, Steam Heating 40min, makes wheat juice occur protein precipitation, is then cooled to 20～30 ℃, uses double-deck Filter paper filtering.
With graduated cylinder, measure 220mL wheat juice and pour in SCOTT bottle, according to wort concentration, add appropriate syrup, be made into pol and be 12% wheat juice and dissolve and mix, screw bottle cap, then put into pot, wet sterilization 20min after boiling, cooling standby.
Yeast is prepared: the yeast paste (2～4 generation) of production and application is got in sterile working, fetches in rear placement refrigerator, in 8hr, uses.Get the 40mL centrifuge tube for alcohol swab wiping of drawing, move into yeast paste, in the centrifugal 10min of 4000rpm, supernatant inclines; Get the 40mL centrifuge tube for alcohol swab wiping of drawing, take the yeast paste 1.10g after centrifugal.
The wheat juice preparing is added to about 10-20mL in the centrifuge tube that weighs yeast, after using vortex oscillator fully to shake, rejoin in wheat juice, and utilize wheat juice to rinse 3 times centrifuge tube, washing fluid all returns in SCOTT bottle; Screw oxygenation.
Fermentation: oxygenation is complete, pours wheat juice in dry, clean EBC color comparison tube into after wheat juice shakes gently, uses masking foil that mouth is sealed, and the incubator that is uprightly placed in 9.5 ℃ is cultivated 5 days, carries out yeast counts, and calculates PYF value (%), as table 2.
Table 2:
| Numbering | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| PYF(％) | 159 | 120 | 105 | 102 | 98 | 82 | 64 | 53 | 29 | 28 | 16 | 13 |
Three, mapping is calculated
Water soluble pentosan and PYF related coefficient, as described in Figure 1, by above data and Fig. 1, can be found out, use prediction Fructus Hordei Germinatus that the present invention develops to cause method and the traditional zymotic method that yeast flocculates in advance and there is higher correlativity, can answer by Fructus Hordei Germinatus Water soluble pentosan content back the size of PYF value.
Embodiment 2:
Utilize detection method of the present invention, to adding wheat Metcalfe1212, Australia wheat Vlamingh1302, tri-kind wheat process processed Water soluble pentosan Changing Patterns of Australia wheat Gairdner1306 are analyzed, experimental data is as table 3:
Table 3: wheat process Water soluble pentosan Changing Pattern processed
By analyzing data, can find out:
Although barley variety is different, Water soluble pentosan content no significant difference in raw material barley, the PYF of barley and barley variety are irrelevant;
Water soluble pentosan content, along with wheat process processed constantly increases, reaches peak value in second or the 3rd day in germination, starts afterwards to reduce, and in wheat process processed, the PYF of Fructus Hordei Germinatus first raises, then reduces.
By example 2, can be found out, utilize detection method of the present invention to carry out Fructus Hordei Germinatus PYF detection to barley, wheat process processed and finished product Fructus Hordei Germinatus, for improvement and the optimization of wheat technique processed provides Data support.
Claims (6)
1. detect in barley or Fructus Hordei Germinatus and cause the method that yeast shifts to an earlier date the coherency factor (PYF), it is characterized in that the step of described method is as follows:
Step 1: get the sample that moisture is Wc, pulverize, weigh sample W
2for 25g is in the triangular flask of 500ml, add 300mL deionized water, mix; The 150rpm 1-2h that vibrates, adds water constant weight to W
1for 350g, Filter paper filtering, filtered fluid detects proportion S
g, extract Me; Get filtered fluid 10mL and add 30mL absolute ethyl alcohol, mix; Ice bath alcohol precipitation reaction 1h; The centrifugal 10min of sample 10000rpm after reaction, removes supernatant, and precipitation adds 10mL deionized water to mix, and 85 ℃ of water-bath 2h dissolve; After dissolving, 10000rpm carries out centrifugal removal precipitation, and the freezing preservation of supernatant is standby;
Step 2: drawing standard curve, utilize wood sugar and the phloroglucin chromogenic reaction under acid condition, form red material, measure content, computing formula is as follows:
Y＝a X
552+b
Wherein: X
552for 552nm up-to-date style product absorbance; A is the slope of typical curve; B is the intercept of typical curve; Y is xylose concentration in supernatant, mg/L;
Wherein: G is Water soluble pentosan content in sample, mg/100g over dry Fructus Hordei Germinatus; N is extension rate; Y is xylose concentration in supernatant, mg/L; Wc is the moisture of sample, %; Me is the extract content of brewer's wort, g; S
gproportion for brewer's wort; W
1for sample wheat juice weight, g; W
2for example weight, g; 0.65 is wood sugar and xylan conversion coefficient.
2. in detection barley according to claim 1 or Fructus Hordei Germinatus, cause the method that yeast shifts to an earlier date the coherency factor (PYF), it is characterized in that the sample described in step 1 is barley or Fructus Hordei Germinatus.
3. in detection barley according to claim 1 or Fructus Hordei Germinatus, cause the method that yeast shifts to an earlier date the coherency factor (PYF), it is characterized in that in step 1, in ice bath alcohol precipitation course of reaction, within every 20 minutes, shaking up once.
4. in detection barley according to claim 1 or Fructus Hordei Germinatus, cause the method that yeast shifts to an earlier date the coherency factor (PYF), it is characterized in that in step 1, sample is pulverized employing EBC comminutor.
5. in detection barley according to claim 1 or Fructus Hordei Germinatus, cause the method that yeast shifts to an earlier date the coherency factor (PYF), it is characterized in that in step 2, wood sugar Specification Curve of Increasing method is: the configuration of 500mg/L wood sugar standard solution: 50mg wood sugar is settled to 100mL with deionized water; Take out respectively 1.0,2.0,3.0,4.0,6.0, wood sugar standard solution described in 8.0mL, be diluted to 10mL, the wood sugar standard solution of preparation variable concentrations, get respectively the standard solution 1mL after dilution, add after phloroglucin-glacial acetic acid reactant liquor 5mL, mix boiling water bath reaction 25 minutes, ice bath cessation reaction; The water of take adds reactant liquor as contrast, measures each concentration wood sugar at 552nm absorbance, according to known xylose concentration value, Criterion curve.
6. in detection barley according to claim 1 or Fructus Hordei Germinatus, cause the method that yeast shifts to an earlier date the coherency factor (PYF), it is characterized in that the step of step 2 chromogenic reaction is: get 3 color-comparison tubes and identify respectively, number; Blank: in No. 1 color comparison tube, to add 1ml distilled water; Sample: add 1ml to prepare sample in 2, No. 3 color comparison tubes; In color comparison tube, respectively add phloroglucin-glacial acetic acid reactant liquor 5ml; Immediately 3 color comparison tubes are put into boiling water bath reaction 25 minutes, ice bath cessation reaction; With blank, regulate instrument zero, under 552nm wavelength, detect the light absorption value of testing sample.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105200117A (en) * | 2015-10-15 | 2015-12-30 | 燕京啤酒(桂林漓泉)股份有限公司 | Method for detecting PYF (premature yeast flocculation) factor in malt |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5047351A (en) * | 1986-09-01 | 1991-09-10 | Fuji Photo Film Co., Ltd. | Optical end-point type analytical method |
| WO2008053603A1 (en) * | 2006-11-02 | 2008-05-08 | Kirin Beer Kabushiki Kaisha | Method of improving fermentability of early flocculating malt |
| CN101349636A (en) * | 2007-07-16 | 2009-01-21 | 青岛啤酒股份有限公司 | Method for testing germinated barley flocculating degree |
| CN101451958A (en) * | 2008-12-29 | 2009-06-10 | 东莞泛亚太生物科技有限公司 | Xylanase activity detecting method |
| CN101493420A (en) * | 2009-02-24 | 2009-07-29 | 华南理工大学 | Method for rapidly measuring pentoses and hexose content in hemicellulose extract |
| JP2011072208A (en) * | 2009-09-29 | 2011-04-14 | Kirin Brewery Co Ltd | Method for producing barley malt having suppressed early flocculation of yeast |
| JP2011125285A (en) * | 2009-12-18 | 2011-06-30 | Kirin Brewery Co Ltd | Method for quickly measuring factor causing early flocculation of yeast, and measurement apparatus therefor |
-
2014
- 2014-07-03 CN CN201410314223.5A patent/CN104062293B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5047351A (en) * | 1986-09-01 | 1991-09-10 | Fuji Photo Film Co., Ltd. | Optical end-point type analytical method |
| WO2008053603A1 (en) * | 2006-11-02 | 2008-05-08 | Kirin Beer Kabushiki Kaisha | Method of improving fermentability of early flocculating malt |
| CN101349636A (en) * | 2007-07-16 | 2009-01-21 | 青岛啤酒股份有限公司 | Method for testing germinated barley flocculating degree |
| CN101451958A (en) * | 2008-12-29 | 2009-06-10 | 东莞泛亚太生物科技有限公司 | Xylanase activity detecting method |
| CN101493420A (en) * | 2009-02-24 | 2009-07-29 | 华南理工大学 | Method for rapidly measuring pentoses and hexose content in hemicellulose extract |
| JP2011072208A (en) * | 2009-09-29 | 2011-04-14 | Kirin Brewery Co Ltd | Method for producing barley malt having suppressed early flocculation of yeast |
| JP2011125285A (en) * | 2009-12-18 | 2011-06-30 | Kirin Brewery Co Ltd | Method for quickly measuring factor causing early flocculation of yeast, and measurement apparatus therefor |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105200117A (en) * | 2015-10-15 | 2015-12-30 | 燕京啤酒(桂林漓泉)股份有限公司 | Method for detecting PYF (premature yeast flocculation) factor in malt |
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