CN104034709A - Optical system of fluoroanalyzer - Google Patents
Optical system of fluoroanalyzer Download PDFInfo
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- CN104034709A CN104034709A CN201410250599.4A CN201410250599A CN104034709A CN 104034709 A CN104034709 A CN 104034709A CN 201410250599 A CN201410250599 A CN 201410250599A CN 104034709 A CN104034709 A CN 104034709A
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- objective lens
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- mirror
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Abstract
The invention belongs to the technical field of optics, and in particular relates to an optical system of a fluoroanalyzer. The optical system comprises a laser reflector base, a laser reflector, a reflector, a reflector pressing plate, a reflector base, a lens base, an objective lens group and an image lens group, wherein the laser reflector is fixed on the laser reflector base; the laser reflector base is fixed on the reflector base, the reflector pressing plate and the reflector base are used for fixing a reflector on the front of the lens base, and the center of the reflector is provided with a 45-degree inclined hole; the objective lens group is fixed on the lower part of the reflector base, the image lens group is fixed on the lower part of the lens base, and the image mirror group is fixed on the rear of the lens base and comprises a dichroscope for detecting that a laser beam irradiates the laser reflector to pass through the 45-degree inclined hole of the center of the reflector so as to penetrate through the light of the objective lens group to irradiate the image lens group through the reflection of the reflector. The optical system of the fluoroanalyzer provided by the invention has the advantages that the structure is simple and compact, and the accuracy and reliability of a detection system are remarkably improved.
Description
Technical field
The invention belongs to fluorescence analysis, optical light splitting technology field, relate to especially a kind of fluorescence analyser optical system.
Background technology
At present, existing fluorescence analyser major defect is, Systems for optical inspection complex structure, resolution and accuracy of detection to detected sample are low, be difficult to reach to the requirement of detection of biological samples precision index, common fluorescence analyser adopts the light with certain bandwidth as detection light source, detected biological specimen to be irradiated conventionally, but the detected light that biological specimen inspires mixes in light path with former detection light conventionally, cause detection system resolution very low, thereby reduce the complete machine precision index of fluorescence analyser, therefore provide a kind of fluorescence analyser optical system that improves fluorescence analyser accuracy of detection and reliability significant.
Summary of the invention
In order to solve existing fluorescence analyser Systems for optical inspection complex structure, to resolution and the low technical matters of accuracy of detection of detected sample, the present invention proposes a kind of fluorescence analyser optical system that can improve fluorescence analyser accuracy of detection and reliability.
The technical solution adopted in the present invention is as follows:
A kind of fluorescence analyser optical system, comprise: laser reflection microscope base, laser mirror, catoptron, catoptron pressing plate, mirror unit, microscope base, objective lens, picture mirror group, wherein said laser mirror is fixed on described laser reflection microscope base, described laser reflection microscope base is fixed on described mirror unit, described catoptron pressing plate and described mirror unit are fixed on described catoptron the front portion of described microscope base, and described catoptron center has the inclined hole of one 45 °, described objective lens is fixed on the bottom of described microscope base, describedly be fixed on the back of described microscope base and described as comprising a dichroscope in mirror group as mirror group, detection laser beam incides described objective lens through 45 ° of inclined holes at described catoptron center after being irradiated to described laser mirror, see through the light of described objective lens again by being irradiated in described picture mirror group after described catoptron reflection.
Further, described as mirror group successively by as mirror group lens, as mirror group spacer ring, as mirror group lens, as the little microscope base of mirror group, dichroscope be fixed and form as mirror group trim ring.
Further, described objective lens is pressed abd fixed on laser beam expanding lens, objective lens spacer ring, objective lens lens, objective lens spacer ring and objective lens lens in the little microscope base of objective lens and forms successively by objective lens trim ring.
Further, described laser mirror is fixed on described laser reflection microscope base by bonding method, and described laser reflection microscope base is fixed by screws on described mirror unit.
The groundwork principle of fluorescence analyser optical system of the present invention is: utilize dichroscope to carry out filtering to entering as the light (being made up of former detection laser and biological specimen exciting light) that mixes in mirror group, obtaining biological specimen exciting light is imaging.
The invention has the beneficial effects as follows: this fluorescence analyser optical system, solve fluorescence analyser Systems for optical inspection complex structure, to resolution and the low problem of accuracy of detection of detected sample, it is simple in structure, compact, has significantly improved detection system precision and reliability.
Brief description of the drawings
Fig. 1 is fluorescence analyser optical system structure assembling schematic diagram.
Fig. 2 is fluorescence analyser optical system structure explosive view.
In figure: 1. laser reflection microscope base, 2. laser mirror, 3. screw, 4. catoptron, 5. catoptron pressing plate, 6. microscope base, 7. objective lens trim ring, 8. laser beam expanding lens, 9. objective lens spacer ring, 10. objective lens lens, the little microscope base of 11. objective lens, 12. mirror units, 13. picture mirror group spacer rings, 14. picture mirror group lens, the little microscope base of 15. picture mirror group, 16. dichroscopes, 17. picture mirror group trim rings.
Embodiment
Below in conjunction with Fig. 1-Fig. 2, the specific embodiment of the present invention is elaborated.
Fluorescence analyser optical system of the present invention comprises: laser reflection microscope base 1, laser mirror 2, catoptron 4, catoptron pressing plate 5, mirror unit 12, microscope base 6, objective lens, as mirror group, successively objective lens lens 10, objective lens spacer ring 9, objective lens lens 10, objective lens spacer ring 9, laser beam expanding lens 8 are packed into and utilize objective lens trim ring 7 compression that is fixed after the little microscope base 11 of objective lens, and little the objective lens assembling microscope base 11 is screwed in microscope base 6 and forms objective lens, objective lens is used for observing biological specimen.
Dichroscope 16 is packed into as the rear utilization of the little microscope base 15 of mirror group and is fixed and clamped as mirror group trim ring 17, successively will be as mirror group lens 14, as mirror group spacer ring 13, pack into after microscope base 6 as mirror group lens 14, utilize as the little microscope base 15 of mirror group be fixed compress after group imaging lens group.
Laser mirror 2 is pasted and is fixed on laser reflection microscope base 1, then laser reflection microscope base 1 is fixed on mirror unit 12 by screw 3.
Utilize catoptron pressing plate 5 and mirror unit 12 that catoptron 4 is fixed on microscope base 6 by screw 3, debug 45 ° of inclined hole centers of catoptron 4 and objective lens center superposition through optics.
When detection, detection laser beam horizontal direction impinges perpendicularly on laser beam expanding lens 8 through catoptron 4 central small hole after being irradiated to 45 ° of laser mirrors of putting 2, laser after expanding is irradiated to the light that inspires specific wavelength on whole detected biological specimen after objective lens lens 10 optically focused, this exciting light and former laser are mixed in together, successively see through objective lens lens 10 and laser beam expanding lens 8, and after being reflected by catoptron 4, be irradiated in picture mirror group, this mixes light and is irradiated on dichroscope 16 after as mirror group lens 14 optically focused, utilize dichroscope 16 to filter after former detection laser, only having exciting light to see through as the light hurdle on mirror group trim ring 17 is irradiated on photomultiplier thereafter, convert optical information to telecommunications breath, finally can reach the object of imaging.
Claims (4)
1. a fluorescence analyser optical system, it is characterized in that: comprising: laser reflection microscope base (1), laser mirror (2), catoptron (4), catoptron pressing plate (5), mirror unit (12), microscope base (6), objective lens, picture mirror group, wherein said laser mirror (2) is fixed on described laser reflection microscope base (1), described laser reflection microscope base (1) is fixed on described mirror unit (12), described catoptron pressing plate (5) and described mirror unit (12) are fixed on described catoptron (4) front portion of described microscope base (6), and described catoptron (4) center has the inclined hole of one 45 °, described objective lens is fixed on the bottom of described microscope base (6), describedly be fixed on the back of described microscope base (6) and described as comprising a dichroscope in mirror group as mirror group, detection laser beam incides described objective lens through 45 ° of inclined holes at described catoptron (4) center after being irradiated to described laser mirror (2), see through the light of described objective lens again by being irradiated in described picture mirror group after described catoptron (4) reflection.
2. fluorescence analyser optical system as claimed in claim 1, is characterized in that: described picture mirror group successively by picture mirror group lens (14), as mirror group spacer ring (13), as mirror group lens (14), be fixed formation as the little microscope base of mirror group (15), dichroscope (16) and picture mirror group trim ring (17).
3. fluorescence analyser optical system as claimed in claim 1 or 2, is characterized in that: described objective lens is pressed abd fixed on laser beam expanding lens (8), objective lens spacer ring (9), objective lens lens (10), objective lens spacer ring (9) and objective lens lens (10) successively in the little microscope base of objective lens (11) and is formed by objective lens trim ring (7).
4. fluorescence analyser optical system as claimed in claim 1 or 2, it is characterized in that: described laser mirror (2) is fixed on described laser reflection microscope base (1) by bonding method, it is upper that described laser reflection microscope base (1) is fixed by screws in described mirror unit (12), and described objective lens screws in described microscope base (6) by the little microscope base of described objective lens (11) screw thread pair.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410250599.4A CN104034709A (en) | 2014-06-06 | 2014-06-06 | Optical system of fluoroanalyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410250599.4A CN104034709A (en) | 2014-06-06 | 2014-06-06 | Optical system of fluoroanalyzer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104034709A true CN104034709A (en) | 2014-09-10 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410250599.4A Pending CN104034709A (en) | 2014-06-06 | 2014-06-06 | Optical system of fluoroanalyzer |
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| CN (1) | CN104034709A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106772977A (en) * | 2016-12-26 | 2017-05-31 | 云南北方驰宏光电有限公司 | One kind is infrared to merge microlens and assembly method with the double light of visible ray |
| CN106989525A (en) * | 2017-04-10 | 2017-07-28 | 天津清芸主力能源科技有限公司 | A kind of disc type solar energy photo-thermal power generation amplitude transformer device |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1092864A (en) * | 1992-11-27 | 1994-09-28 | 英国石油国际有限公司 | Identify the method for liquid petroleum product |
| US5926271A (en) * | 1995-12-20 | 1999-07-20 | Zeta Technology | Laser-induced fluorescence detector having a capillary detection cell and method for identifying trace compounds implemented by the same device |
| CN1515923A (en) * | 2003-01-02 | 2004-07-28 | 财团法人工业技术研究院 | Light uniformization microprojector device |
| CN102818794A (en) * | 2012-07-23 | 2012-12-12 | 苏州生物医学工程技术研究所 | Biological fluorescence microscopic detection instrument |
| CN103134784A (en) * | 2013-02-05 | 2013-06-05 | 华中科技大学 | Optical fiber living body fluorescence excitation spectral imaging device |
-
2014
- 2014-06-06 CN CN201410250599.4A patent/CN104034709A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1092864A (en) * | 1992-11-27 | 1994-09-28 | 英国石油国际有限公司 | Identify the method for liquid petroleum product |
| US5926271A (en) * | 1995-12-20 | 1999-07-20 | Zeta Technology | Laser-induced fluorescence detector having a capillary detection cell and method for identifying trace compounds implemented by the same device |
| CN1515923A (en) * | 2003-01-02 | 2004-07-28 | 财团法人工业技术研究院 | Light uniformization microprojector device |
| CN102818794A (en) * | 2012-07-23 | 2012-12-12 | 苏州生物医学工程技术研究所 | Biological fluorescence microscopic detection instrument |
| CN103134784A (en) * | 2013-02-05 | 2013-06-05 | 华中科技大学 | Optical fiber living body fluorescence excitation spectral imaging device |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106772977A (en) * | 2016-12-26 | 2017-05-31 | 云南北方驰宏光电有限公司 | One kind is infrared to merge microlens and assembly method with the double light of visible ray |
| CN106989525A (en) * | 2017-04-10 | 2017-07-28 | 天津清芸主力能源科技有限公司 | A kind of disc type solar energy photo-thermal power generation amplitude transformer device |
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Application publication date: 20140910 |