CN104027852A

CN104027852A - Surface modifying method for biological material

Info

Publication number: CN104027852A
Application number: CN201410291528.9A
Authority: CN
Inventors: 潘长江; 王亚楠; 刘涛; 侯彦华; 张临财
Original assignee: Huaiyin Institute of Technology
Current assignee: Huai'an qingjiangpu district market supervision comprehensive service center
Priority date: 2014-06-26
Filing date: 2014-06-26
Publication date: 2014-09-10
Anticipated expiration: 2034-06-26
Also published as: CN104027852B

Abstract

The invention discloses a surface modifying method for a biological material. The surface modifying method comprises the step of carrying out oxygen gas plasma activation treatment on the material surface and then carrying out surface self-assembling; then, alternatively immobilizing chemical molecules with different functional groups in sequence by adopting a micro-contact printing technology, so that the biological active molecules with different biological functions are immobilized on the material surface to obtain the biological material with multifunctional biological activity. The method disclosed by the invention is adopted to treat the material surface, so that multifunctional biological activity is provided for the material surface, and therefore, the surface modifying method is applied to the fields such as biological materials, tissue engineering and implantable medical device research.

Description

The surface modifying method of biomaterial

Technical field

The invention belongs to technical field of biological material, be specifically related to a kind of surface modifying method of biomaterial.

Background technology

Biomaterial has important application at aspects such as improving health and prevent disease, the biomaterial of implant into body inevitably can with tissue and the body fluid generation interfacial interaction of implant site, thereby cause a series of side reaction and clinical complication, cause graft failure, therefore, the surface property of material often has conclusive effect to implanting success or failure.

Embedded material and the interaction of implanting between surrounding are very complicated, therefore, the surface modification of biomaterial must be considered material and the multiple interaction mechanism of implanting surrounding comprehensively, by building multifunctional bio-active surface, carry out the interface biological behaviour between controlled material and tissue or body fluid, and then essence ground improves performance and the function of embedded material.

At present, the multifunctional modification method of material surface is mainly to adopt the single molecule with multiple biological function to carry out modification, or fix two or more bioactive molecules at material surface, fixing method be mainly physisorphtion (as, self assembly layer by layer) and chemical method (as, order grafting, grafting simultaneously etc.).But these methods mainly still depend on conventional surface modification technology, fixing biomolecule activity is affected, and between molecule, also can influence each other, therefore, be difficult to realize the Effective Regulation of surrounding tissue and the performance and the function that fundamentally improve embedded material; Simultaneously, in the process of the fixing multiple bioactive molecule of conventional surface modification technology, reacting to each other between biomolecule (as, order grafting biomolecule) and the covering benefit of front and back fixing biological molecules (as, self assembly layer by layer) can cause influencing each other between fixing various biomolecules, be difficult to give full play to the biological activity of different kinds of molecules, and surface property is difficult to regulation and control simultaneously.

Summary of the invention

The object of the invention is to: the surface modifying method that proposes a kind of biomaterial, by the method, build the material surface with multifunctional bio-active, give material multifunctional bio-active, thereby the interfacial interaction between Effective Regulation material and implantation host, improves the biocompatibility of material and is implanted to power.

The technical solution that the present invention adopts is: first material surface is carried out after oxygen gas plasma activation processing to self assembly thiazolinyl silane again, then adopt Microcontact printing fixedly to there is successively the chemical molecular of different functional groups, and then successively the bioactive molecule with difference in functionality is fixed on to material surface, obtain the biomaterial with multifunctional bio-active.

Wherein, the discharge mode of described oxygen gas plasma activation processing is radio-frequency discharge mode, and discharge power is 50W～300W, and the processing time is 10～30 minutes.

Wherein, described surface self-organization is surface self-organization thiazolinyl silane, thiazolinyl silane is allyltrimethoxysilanis, allyltriethoxysilane or allyltrichlorosilane, the self-assembling reaction time is 6～24 hours, solution concentration is 5mM～100mM, and solvent is a kind of in dichloromethane, ethanol, toluene, dimethyl sulfoxide, dimethyl formamide.

Wherein, described Microcontact printing is to adopt ribbon micrographics seal on the matrix surface of self assembly thiazolinyl silane, to replace successively fixed compound 1, compound 2 and compound 3, compound 1 is the chemical molecular (3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propanoic acid and analog thereof) that two ends are respectively sulfydryl (SH) and carboxyl (COOH), and compound 2 is that two ends are respectively sulfydryl (SH) and azido group (N ₃) chemical molecular (N-(2-azido ethyl)-3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propionic acid amide. and analog thereof), compound 3 is that two ends are respectively amino (NH ₂) and the chemical molecular (2-(2-(2-amino ethoxy) ethyoxyl) the ethyl carbamic acid tert-butyl ester and analog thereof) of t-butyl carbamate base (Boc).

Wherein, described micro-contact printing fixed compound 1 is by compound 1(20～80mM) and 2, the alcohol mixed solution of 2-dimethoxy-phenyl 1-Phenylethanone. (DMPA, 10～40mM) is added drop-wise to seal surface, fully adsorbs after 1～3 minute and slowly dries up with argon; Again seal is fully contacted with the material surface of two key modifications, after the irradiation under ultraviolet ray 1～3min of room temperature with 365nm, take off seal, with drying up with Ar after alcoholic solution ultrasonic cleaning; Matrix is further immersed into EDC(10～40mM)/NHS(5～20mM) mixed solution in react 1～4 hour, after distilled water cleans, with Ar, dry up.

Wherein, described micro-contact printing fixed compound 2 is by compound 2(20～80mM) and DMPA(10～40mM) alcohol mixed solution be added drop-wise to seal surface, fully adsorb after 1～3 minute and dry up with Ar; Seal is fully contacted with matrix surface, irradiation under ultraviolet ray 1～3min of 365nm for room temperature, carefully takes off seal again, and matrix is with drying up with Ar after alcoholic solution ultrasonic cleaning.

Wherein, described micro-contact printing fixed compound 3 is by compound 3(5～20mM) alcoholic solution be added drop-wise to PDMS seal surface, fully adsorb after 1～3 minute and dry up with Ar; Again seal is fully contacted to 1～3min with matrix surface, carefully take off seal, matrix is room temperature reaction 10～60min in 90% TFA solution with being immersed into mass concentration after alcoholic solution ultrasonic cleaning, after cleaning, dries up.

Wherein, the described bioactive molecule fixedly successively with different biological functions is ,-N ₃group position is fixing containing amino bioactive molecule, the fixing bioactive molecule containing sulfydryl (SH) in C=C position ,-NH ₂carboxylic bioactive molecule is fixed in position ,-NHS group position is fixing containing amino bioactive molecule.Containing amino bioactive molecule, be hirudin, protein (albumin, antithrombase, Fibrinogen etc.) or polypeptide (arginine-glycine-aspartic acid (RGD), arginine-glutamic acid-aspartic acid-valine (REDV) etc.); Bioactive molecule containing sulfydryl (SH) is mercapto-polyglycol (SH-PEG); Carboxylic bioactive molecule is extracellular matrix protein (fibronectin, laminin，LN, bone morphogenetic protein etc.), cell growth factor (endothelial cell growth factor (ECGF) (VEGF) etc.), albumin or heparin.

Wherein, the fixing bioactive molecule containing sulfydryl of material surface position of double bond is that polyfunctional group surface modifying material is immersed into bioactive molecule (2～40mM) and DMPA(2～20mM) mixed solution in, 365nm irradiation under ultraviolet ray 3～5min, fully cleans with distilled water after taking-up.

Wherein, material surface-NH ₂the fixing carboxylic bioactive molecule in position is that polyfunctional group surface modifying material is immersed into EDC(5～40mM)/NHS(5～20mM) fully reaction 2～8 hours in the mixed solution of/bioactive molecule (2～10mg/ml), after taking-up, with distilled water, fully clean.

Wherein, material surface-N ₃position is fixing is that polyfunctional group surface modifying material is immersed in the bioactive molecule solution of 1～10mg/ml containing amino bioactive molecule, and 365nm ultra-vioket radiation 3～5 minutes, fully cleans with distilled water after taking-up.

Wherein, material surface-NHS group position is fixing is that the material of surface modification is immersed in bioactive molecule (1～10mg/ml) solution containing amino bioactive molecule, and stirring at normal temperature reaction 2～8 hours is fully cleaned rear dry with distilled water.

the present invention has the following advantages:

(1) adopt Microcontact printing to build multifunctional bio-active surface, at the fixing multiple bioactive molecule of material surface, by the synergism between multiple bioactive molecule, improve performance and the function of biomaterial comprehensively.

(2) emphasize micrographics that material surface bioactive substance and bioactive substance the form regulating and controlling effect to embedded material surrounding, by regulating the size and dimension of micrographics to control fixed amount and the topological structure of material surface biomolecule.

(3) by microcell, be controlled at the fixing different bioactive molecule in different regions, eliminate influencing each other between molecule, fixedly do not consume mutual chemical active radical during various biomolecules, give full play to the functional activity of various biomolecules.

(4) by the different combinations of biomolecules of choose reasonable, can build the material surface with different multifunction activities, adapt to different tissues reaction needed.

(5) by changing the size of micrographics and selecting suitable bioactive molecule combination can obtain having different multi-functional bioactivity surfaces, and then adapt to the different interfacial interactions of implanting environment, improve the biocompatibility of material and be implanted to power.

(6) the method applied in the present invention can be applied to the surface treatment of nearly all biomaterial, is widely used in the surface-treated every field of biomaterial, organizational project and Implantable Medical Device.

Accompanying drawing explanation

Fig. 1 is the fundamental reaction step schematic diagram of biomaterial multifunctional bio-active surface construction, in figure, comprises the following steps:

(1) surface active and self assembly: material surface carries out after oxygen gas plasma activation processing self assembly thiazolinyl silane again, at material surface, introduces the two key groups of C=C;

(2) polyfunctional group chemical surface builds: adopt Microcontact printing to replace successively the fixedly chemical molecular of different functional groups, build multi-functional chemical surface;

(3) surface in situ bioactive molecule is fixed: adopt the surface in situ chemistry fixing means fixing different bioactive molecules in different functional groups region successively, obtain having the biomaterial of multifunctional bio-active.

The specific embodiment

In order further to explain technical scheme of the present invention, below by specific embodiment, the present invention will be described in detail, and these embodiment can not be interpreted as it is the restriction to technical scheme.

embodiment 1:according to following steps, material surface is processed

(1) surface active and self assembly: after sample fully cleans, with oxygen gas plasma, process, discharge power is 50W, and the processing time is 5 minutes; Sample after processing is immersed in the alcoholic solution of allyltrimethoxysilanis of 5mM and reacts 6 hours, after fully cleaning successively, dries after taking-up with ethanol and distilled water.

(2) polyfunctional group chemical surface builds: first, by compound 1(20mM, 1ml) and DMPA(10mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorb after 1 minute and slowly dry up afterwards and fully contact with the material surface of two key modifications with argon, after 365nm irradiation under ultraviolet ray 1min, take off seal, with drying up with Ar after alcoholic solution ultrasonic cleaning, further be immersed into EDC(10mM, 2ml)/NHS(5mM, in mixed solution 2ml), react 1 hour, distilled water dries up with Ar after cleaning; Then, by compound 2(20mM, 1ml) and DMPA(10mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorbs after 1 minute and dries up afterwards and fully contact with matrix surface with Ar, 365nm irradiation under ultraviolet ray 1min, take off seal, matrix is with drying up with Ar after alcoholic solution ultrasonic cleaning; Finally, by compound 3(5mM, alcoholic solution 1ml) is added drop-wise to PDMS seal surface, adsorb and with Ar, dry up after 1 minute, seal is fully contacted to 1min with matrix surface, carefully take off seal, matrix, with being immersed into room temperature reaction 10min in 90% trifluoroacetic acid (TFA) solution after alcoholic solution ultrasonic cleaning, dries up after cleaning; Compound 1 is 3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propanoic acid, compound 2 is N-(2-azido ethyl)-3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propionic acid amide., and compound 3 is 2-(2-(2-amino ethoxy) ethyoxyl) the ethyl carbamic acid tert-butyl ester.

(3) surface in situ bioactive molecule is fixed: first, at the fixing mercapto-polyglycol (SH-PEG) of C=C position of double bond, polyfunctional group surface modifying material is immersed into SH-PEG(2mM, 2ml) and DMPA(2mM, in mixed solution 2ml), 365nm irradiation under ultraviolet ray 3min, fully cleans with distilled water after taking-up; Then, at-NH ₂carboxylic bioactive molecule is fixed in position, and material is immersed into EDC(5mM, 1ml)/NHS(5mM, 1ml) fully reaction 2 hours in the mixed solution of/heparin (2mg/ml, 2ml), after taking-up, with distilled water, fully clean; Again, at-N ₃position is fixing to be immersed into polyfunctional group surface modifying material in the hirudin solution of 1mg/ml containing amino bioactive molecule, and 365nm ultra-vioket radiation 3 minutes, fully cleans with distilled water after taking-up; Finally ,-NHS group position is fixing to be immersed into the material of surface modification in the fibronectin solution of 1mg/ml containing amino bioactive molecule, and stirring at normal temperature reaction 2 hours, fully cleans rear dry with distilled water.

embodiment 2:according to following steps, material surface is processed

(1) surface active and self assembly: after sample fully cleans, with oxygen gas plasma, process, discharge power is 150W, and the processing time is 20 minutes; Sample after processing is immersed in the alcoholic solution of allyltriethoxysilane of 5mM and reacts 12 hours, after fully cleaning, dries after taking out with ethanol and distilled water;

(2) polyfunctional group chemical surface builds: first, by compound 1(50mM, 1ml) and DMPA(25mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorb after 2 minutes and slowly dry up afterwards and fully contact with the material surface of two key modifications with argon, after 365nm irradiation under ultraviolet ray 2min, take off seal, with drying up with Ar after alcoholic solution ultrasonic cleaning, matrix is further immersed into EDC(20mM, 2ml)/NHS(10mM, in mixed solution 2ml), react 2 hours, distilled water dries up with Ar after cleaning; Then, by compound 2(50mM, 1ml) and DMPA(25mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorbs after 2 minutes and dries up afterwards and fully contact with matrix surface with Ar, 365nm irradiation under ultraviolet ray 2min, take off seal, matrix is with drying up with Ar after alcoholic solution ultrasonic cleaning; Finally, by compound 3(10mM, alcoholic solution 1ml) is added drop-wise to PDMS seal surface, adsorb and with Ar, dry up after 2 minutes, seal is fully contacted to 2min with matrix surface, carefully take off seal, matrix, with being immersed into room temperature reaction 30min in 90% TFA solution after alcoholic solution ultrasonic cleaning, dries up after cleaning; Compound 1 is 3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propanoic acid, compound 2 is N-(2-azido ethyl)-3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propionic acid amide., and compound 3 is 2-(2-(2-amino ethoxy) ethyoxyl) the ethyl carbamic acid tert-butyl ester.

(3) surface in situ bioactive molecule is fixed: first, at the fixing mercapto-polyglycol of C=C position of double bond, polyfunctional group surface modifying material is immersed into SH-PEG(20mM, 2ml) and DMPA(10mM, in mixed solution 2ml), 365nm irradiation under ultraviolet ray 4min, fully cleans with distilled water after taking-up; Then, at-NH ₂carboxylic bioactive molecule is fixed in position, and material is immersed into EDC(20mM, 1ml)/NHS(10mM, 1ml) fully reaction 4 hours in the mixed solution of/albumin (5mg/ml, 2ml), after taking-up, with distilled water, fully clean; Again, at-N ₃position is fixing to be immersed into polyfunctional group surface modifying material in the fibrinogen solution of 5mg/ml containing amino bioactive molecule, and 365nm ultra-vioket radiation 4 minutes, fully cleans with distilled water after taking-up; Finally, fixing containing amino bioactive molecule in-NHS group position, the material of surface modification being immersed into VEGF(5mg/ml) in solution, stirring at normal temperature reaction 4 hours, fully cleans rear dry with distilled water.

embodiment 3:according to following steps, material surface is processed

(1) surface active and self assembly: after sample fully cleans, with oxygen gas plasma, process, discharge power is 300W, and the processing time is 30 minutes; Sample after processing is immersed in the toluene solution of 5mM allyltrichlorosilane and reacts 24 hours, after fully cleaning, dries after taking out with toluene, acetone and distilled water;

(2) polyfunctional group chemical surface builds: first, by compound 1(80mM, 1ml) and DMPA(40mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorb after 3 minutes and slowly dry up afterwards and fully contact with the material surface of two key modifications with Ar gas, after 365nm irradiation under ultraviolet ray 3min, take off seal, with drying up with Ar after alcoholic solution ultrasonic cleaning, matrix is further immersed into EDC(40mM, 2ml)/NHS(20mM, in mixed solution 2ml), react 4 hours, distilled water dries up with Ar after cleaning; Then, by compound 2(80mM, 1ml) and DMPA(40mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorbs after 3 minutes and dries up afterwards and fully contact with matrix surface with Ar, 365nm irradiation under ultraviolet ray 3min, take off seal, matrix is with drying up with Ar after alcoholic solution ultrasonic cleaning; Finally, by compound 3(20mM, 1ml) solution is added drop-wise to PDMS seal surface, adsorb and with Ar, dry up after 3 minutes, seal is fully contacted to 3min with matrix surface, carefully take off seal, matrix, with being immersed into room temperature reaction 60min in 90% trifluoroacetic acid (TFA) solution after alcoholic solution ultrasonic cleaning, dries up after cleaning; Compound 1 is 3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propanoic acid, compound 2 is N-(2-azido ethyl)-3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propionic acid amide., and compound 3 is 2-(2-(2-amino ethoxy) ethyoxyl) the ethyl carbamic acid tert-butyl ester;

(3) surface in situ bioactive molecule is fixed: first, at the fixing mercapto-polyglycol of C=C position of double bond, polyfunctional group surface modifying material is immersed into mercapto-polyglycol (40mM, 2ml) and DMPA(20mM, in mixed solution 2ml), 365nm irradiation under ultraviolet ray 5min, fully cleans with distilled water after taking-up; Then, at-NH ₂carboxylic bioactive molecule is fixed in position, and material is immersed into EDC(40mM, 1ml)/NHS(20mM, 1ml) fully reaction 8 hours in the mixed solution of/Antithrombin Ⅲ (10mg/ml, 2ml), after taking-up, with distilled water, fully clean; Again, at-N ₃position is fixing to be immersed into polyfunctional group surface modifying material in the hirudin solution of 10mg/ml containing amino bioactive molecule, and 365nm ultra-vioket radiation 5 minutes, fully cleans with distilled water after taking-up; Finally, fixing containing amino bioactive molecule in-NHS group position, the material of surface modification being immersed into REDV(10mg/ml) in solution, stirring at normal temperature reaction 8 hours, fully cleans rear dry with distilled water.

embodiment 4:according to following steps, material surface is processed

(1) surface active and self assembly: after sample fully cleans, with oxygen gas plasma, process, discharge power is 50W, and the processing time is 10 minutes; Sample after processing is immersed in the dimethyl sulphoxide solution of allyltrimethoxysilanis of 50mM and reacts 6 hours, after taking out, with dimethyl sulfoxide, ethanol and distilled water, fully after cleaning, dries successively;

(2) polyfunctional group chemical surface builds: first, by compound 1(20mM, 1ml) and DMPA(10mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorb after 1 minute and slowly dry up afterwards and fully contact with the material surface of two key modifications with argon, after 365nm irradiation under ultraviolet ray 1min, take off seal, with drying up with Ar after alcoholic solution ultrasonic cleaning, matrix is further immersed into EDC(10mM, 2ml)/NHS(5mM, in mixed solution 2ml), react 1 hour, distilled water dries up with Ar after cleaning; Then, by compound 2(20mM, 1ml) and DMPA(10mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorbs after 1 minute and dries up afterwards and fully contact with matrix surface with Ar, 365nm irradiation under ultraviolet ray 1min, take off seal, matrix is with drying up with Ar after alcoholic solution ultrasonic cleaning; Finally, by compound 3(5mM, alcoholic solution 1ml) is added drop-wise to PDMS seal surface, adsorb and with Ar, dry up after 1 minute, seal is fully contacted to 1min with matrix surface, carefully take off seal, matrix, with being immersed into room temperature reaction 10min in 90% trifluoroacetic acid (TFA) solution after alcoholic solution ultrasonic cleaning, dries up after cleaning; Compound 1 is 3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propanoic acid, compound 2 is N-(2-azido ethyl)-3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propionic acid amide., and compound 3 is 2-(2-(2-amino ethoxy) ethyoxyl) the ethyl carbamic acid tert-butyl ester.

(3) surface in situ bioactive molecule is fixed: first, at the fixing mercapto-polyglycol of C=C position of double bond, polyfunctional group surface modifying material is immersed into SH-PEG(2mM, 2ml) and DMPA(2mM, in mixed solution 2ml), 365nm irradiation under ultraviolet ray 3min, fully cleans with distilled water after taking-up; Then, at-NH ₂carboxylic bioactive molecule is fixed in position, and material is immersed into EDC(5mM, 1ml)/NHS(5mM, 1ml)/bone morphogenesis protein-2 (BMP-2,2mg/ml, 2ml) mixed solution in fully reaction 2 hours, after taking-up, with distilled water, fully clean; Again, at-N ₃position is fixing to be immersed into polyfunctional group surface modifying material in the albumin solution of 1mg/ml containing amino bioactive molecule, and 365nm ultra-vioket radiation 3 minutes, fully cleans with distilled water after taking-up; Finally ,-NHS group position is fixing to be immersed into the material of surface modification in the RGD solution of 1mg/ml containing amino bioactive molecule, and stirring at normal temperature reaction 2 hours, fully cleans rear dry with distilled water.

embodiment 5:according to following steps, material surface is processed

(1) surface active and self assembly: after sample fully cleans, with oxygen gas plasma, process, discharge power is 200W, and the processing time is 20 minutes; Sample after processing is immersed in the dimethyl formamide solution of allyltriethoxysilane of 50mM and reacts 12 hours, after taking out, with ethanol and distilled water, fully cleans and dries.

(2) polyfunctional group chemical surface builds: first, by compound 1(50mM, 1ml) and DMPA(25mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorb after 2 minutes and slowly dry up afterwards and fully contact with the material surface of two key modifications with Ar gas, after 365nm irradiation under ultraviolet ray 2min, take off seal, with drying up with Ar after alcoholic solution ultrasonic cleaning, matrix is further immersed into EDC(20mM, 2ml)/NHS(10mM, in mixed solution 2ml), react 2 hours, distilled water dries up with Ar after cleaning; Then, by compound 2(50mM, 1ml) and DMPA(25mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorbs after 2 minutes and dries up afterwards and fully contact with matrix surface with Ar, 365nm irradiation under ultraviolet ray 2min, take off seal, matrix is with drying up with Ar after alcoholic solution ultrasonic cleaning; Finally, by compound 3(10mM, alcoholic solution 1ml) is added drop-wise to PDMS seal surface, adsorb and with Ar, dry up after 2 minutes, seal is fully contacted to 2min with matrix surface, carefully take off seal, matrix, with being immersed into room temperature reaction 30min in 90% TFA solution after alcoholic solution ultrasonic cleaning, dries up after cleaning; Compound 1 is 3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propanoic acid, compound 2 is N-(2-azido ethyl)-3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propionic acid amide., and compound 3 is 2-(2-(2-amino ethoxy) ethyoxyl) the ethyl carbamic acid tert-butyl ester;

(3) surface in situ bioactive molecule is fixed: first, at the fixing mercapto-polyglycol of C=C position of double bond, polyfunctional group surface modifying material is immersed into mercapto-polyglycol (20mM, 2ml) and DMPA(10mM, in mixed solution 2ml), 365nm irradiation under ultraviolet ray 4min, fully cleans with distilled water after taking-up; Then, at-NH ₂carboxylic bioactive molecule is fixed in position, and material is immersed into EDC(20mM, 1ml)/NHS(10mM, 1ml) fully reaction 4 hours in the mixed solution of/heparin (5mg/ml, 2ml), after taking-up, with distilled water, fully clean; Again, at-N ₃position is fixing to be immersed into polyfunctional group surface modifying material in the laminin solution of 5mg/ml containing amino bioactive molecule, and 365nm ultra-vioket radiation 4 minutes, fully cleans with distilled water after taking-up; Finally ,-NHS group position is fixing to be immersed into the material of surface modification in the VEGF solution of 5mg/ml containing amino bioactive molecule, and stirring at normal temperature reaction 4 hours, fully cleans rear dry with distilled water.

embodiment 6:according to following steps, material surface is processed

(1) surface active and self assembly: sample is processed with oxygen gas plasma after fully cleaning, discharge power is 300W, processing time is 30 minutes, sample after processing is immersed in the alcoholic solution of 50mM allyltrimethoxysilanis and reacts 24 hours, after taking out, with ethanol and distilled water, fully after cleaning, dries successively;

(2) polyfunctional group chemical surface builds: first, by compound 1(40mM, 1ml) and DMPA(40mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorb after 3 minutes and slowly dry up afterwards and fully contact with the material surface of two key modifications with argon, after 365nm irradiation under ultraviolet ray 3min, take off seal, with drying up with Ar after alcoholic solution ultrasonic cleaning, matrix is further immersed into EDC(40mM, 2ml)/NHS(20mM, in mixed solution 2ml), react 4 hours, distilled water dries up with Ar after cleaning; Then, by compound 2(80mM, 1ml) and DMPA(40mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorbs after 3 minutes and dries up afterwards and fully contact with matrix surface with Ar, 365nm irradiation under ultraviolet ray 3min, take off seal, matrix is with drying up with Ar after alcoholic solution ultrasonic cleaning; Finally, by the alcoholic solution (20mM of compound 3,1ml) be added drop-wise to PDMS seal surface, adsorb and with Ar, dry up after 3 minutes, seal is fully contacted to 3min with matrix surface, carefully take off seal, matrix, with being immersed into room temperature reaction 60min in 90% TFA solution after alcoholic solution ultrasonic cleaning, dries up after cleaning; Compound 1 is 3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propanoic acid, compound 2 is N-(2-azido ethyl)-3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propionic acid amide., and compound 3 is 2-(2-(2-amino ethoxy) ethyoxyl) the ethyl carbamic acid tert-butyl ester;

(3) surface in situ bioactive molecule is fixed: first, at the fixing mercapto-polyglycol of C=C position of double bond, polyfunctional group surface modifying material is immersed into SH-PEG(40mM, 2ml) and DMPA(20mM, in mixed solution 2ml), 365nm irradiation under ultraviolet ray 5min, fully cleans with distilled water after taking-up; Then, at-NH ₂carboxylic bioactive molecule is fixed in position, and material is immersed into EDC(40mM, 1ml)/NHS(20mM, 1ml) fully reaction 8 hours in the mixed solution of/hirudin (10mg/ml, 2ml), after taking-up, with distilled water, fully clean; Again, at-N ₃position is fixing to be immersed into polyfunctional group surface modifying material in the fibronectin solution of 10mg/ml containing amino bioactive molecule, and 365nm ultra-vioket radiation 5 minutes, fully cleans with distilled water after taking-up; Finally ,-NHS group position is fixing to be immersed into the material of surface modification in the REDV solution of 10mg/ml containing amino bioactive molecule, and stirring at normal temperature reaction 8 hours, fully cleans rear dry with distilled water.

embodiment 7:according to following steps, material surface is processed

(1) surface active and self assembly: after sample fully cleans, with oxygen gas plasma processing, (discharge power is 50W, processing time is 10 minutes), sample after processing is immersed in the dichloromethane solution of 100mM allyltrichlorosilane and reacts 6 hours, after fully cleaning, dries after taking out with dichloromethane, acetone and distilled water;

(2) polyfunctional group chemical surface builds: first, by compound 1(20mM, 1ml) and DMPA(10mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorb after 1 minute and slowly dry up afterwards and fully contact with the material surface of two key modifications with argon, after 365nm irradiation under ultraviolet ray 1min, take off seal, with drying up with Ar after alcoholic solution ultrasonic cleaning, matrix is further immersed into EDC(10mM, 2ml)/NHS(5mM, in mixed solution 2ml), react 1 hour, distilled water dries up with Ar after cleaning; Then, by compound 2(20mM, 1ml) and DMPA(10mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorbs after 1 minute and dries up afterwards and fully contact with matrix surface with Ar, 365nm irradiation under ultraviolet ray 1min, take off seal, matrix is with drying up with Ar after alcoholic solution ultrasonic cleaning; Finally, by compound 3(5mM) alcoholic solution be added drop-wise to PDMS seal surface, adsorb and with Ar, dry up after 1 minute, seal is fully contacted to 1min with matrix surface, carefully take off seal, matrix, with being immersed into room temperature reaction 10min in 90% TFA solution after alcoholic solution ultrasonic cleaning, dries up after cleaning; Compound 1 is 3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propanoic acid, compound 2 is N-(2-azido ethyl)-3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propionic acid amide., and compound 3 is 2-(2-(2-amino ethoxy) ethyoxyl) the ethyl carbamic acid tert-butyl ester;

(3) surface in situ bioactive molecule is fixed: first, at the fixing mercapto-polyglycol of C=C position of double bond, polyfunctional group surface modifying material is immersed into SH-PEG(2mM, 2ml) and DMPA(2mM, in mixed solution 2ml), 365nm irradiation under ultraviolet ray 3min, fully cleans with distilled water after taking-up; Then, at-NH ₂carboxylic bioactive molecule is fixed in position, and material is immersed into EDC(5mM, 1ml)/NHS(5mM, 1ml) fully reaction 2 hours in the mixed solution of/heparin (2mg/ml, 2ml), after taking-up, with distilled water, fully clean; Again, at-N ₃position is fixing to be immersed into polyfunctional group surface modifying material in the hirudin solution of 1mg/ml containing amino bioactive molecule, and 365nm ultra-vioket radiation 3 minutes, fully cleans with distilled water after taking-up; Finally, fixing containing amino bioactive molecule in-NHS group position, material is immersed in the albumin solution of 1mg/ml to stirring at normal temperature reaction 2 hours, with distilled water, fully clean rear dry.

embodiment 8:according to following steps, material surface is processed

(1) surface active and self assembly: sample is processed with oxygen gas plasma after fully cleaning, discharge power is 200W, processing time is 20 minutes, sample after processing is immersed in the toluene solution of 100mM allyltrimethoxysilanis and reacts 12 hours, after taking out, with toluene, acetone and distilled water, fully after cleaning, dries successively;

(2) polyfunctional group chemical surface builds: first, by compound 1(50mM, 1ml) and DMPA(25mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorb after 2 minutes and slowly dry up afterwards and fully contact with the material surface of two key modifications with argon, after 365nm irradiation under ultraviolet ray 2min, take off seal, with drying up with Ar after alcoholic solution ultrasonic cleaning, matrix is further immersed into EDC(20mM, 2ml)/NHS(10mM, in mixed solution 2ml), react 2 hours, distilled water dries up with Ar after cleaning; Then, by compound 2(50mM, 1ml) and DMPA(25mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorbs after 2 minutes and dries up afterwards and fully contact with matrix surface with Ar, 365nm irradiation under ultraviolet ray 2min, take off seal, matrix is with drying up with Ar after alcoholic solution ultrasonic cleaning; Finally, by compound 3(10mM, alcoholic solution 1ml) is added drop-wise to PDMS seal surface, adsorb and with Ar, dry up after 2 minutes, seal is fully contacted to 2min with matrix surface, carefully take off seal, matrix, with being immersed into room temperature reaction 30min in 90% TFA solution after alcoholic solution ultrasonic cleaning, dries up after cleaning; Compound 1 is 3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propanoic acid, compound 2 is N-(2-azido ethyl)-3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propionic acid amide., and compound 3 is 2-(2-(2-amino ethoxy) ethyoxyl) the ethyl carbamic acid tert-butyl ester;

(3) surface in situ bioactive molecule is fixed: first, at the fixing mercapto-polyglycol of C=C position of double bond, polyfunctional group surface modifying material is immersed into mercapto-polyglycol (20mM, 2ml) and DMPA(10mM, in mixed solution 2ml), 365nm irradiation under ultraviolet ray 4min, fully cleans with distilled water after taking-up; Then, at-NH ₂carboxylic bioactive molecule is fixed in position, and material is immersed into EDC(20mM, 1ml)/NHS(10mM, 1ml) fully reaction 4 hours in the mixed solution of/heparin (5mg/ml, 2ml), after taking-up, with distilled water, fully clean; Again, at-N ₃position is fixing to be immersed into polyfunctional group surface modifying material in the hirudin solution of 5mg/ml containing amino bioactive molecule, and 365nm ultra-vioket radiation 4 minutes, fully cleans with distilled water after taking-up; Finally, fixing containing amino bioactive molecule in-NHS group position, the material of surface modification is immersed in the fibronectin solution of 5mg/ml to stirring at normal temperature reaction 4 hours, with distilled water, fully clean rear dry.

embodiment 9:according to following steps, material surface is processed

(1) surface active and self assembly: sample is processed with oxygen gas plasma after fully cleaning, discharge power is 300W, processing time is 30 minutes, sample after processing is immersed in the alcoholic solution of 100mM allyltriethoxysilane and reacts 24 hours, after fully cleaning, dries after taking out with ethanol and distilled water;

(2) polyfunctional group chemical surface builds: first, by compound 1(80mM, 1ml) and DMPA(40mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorb after 3 minutes and slowly dry up afterwards and fully contact with the material surface of two key modifications with argon, after 365nm irradiation under ultraviolet ray 3min, take off seal, with drying up with Ar after alcoholic solution ultrasonic cleaning, matrix is further immersed into EDC(40mM, 2ml)/NHS(20mM, in mixed solution 2ml), react 4 hours, after cleaning, with Ar, dry up; Then, by compound 2(80mM, 1ml) and DMPA(40mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorbs after 3 minutes and dries up afterwards and fully contact with matrix surface with Ar, 365nm irradiation under ultraviolet ray 3min, take off seal, matrix is with drying up with Ar after alcoholic solution ultrasonic cleaning; Finally, by compound 3(20mM, alcoholic solution 1ml) is added drop-wise to PDMS seal surface, adsorb and with Ar, dry up after 3 minutes, seal is fully contacted to 3min with matrix surface, carefully take off seal, matrix, with being immersed into room temperature reaction 60min in 90% trifluoroacetic acid (TFA) solution after alcoholic solution ultrasonic cleaning, dries up after cleaning; Compound 1 is 3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propanoic acid, compound 2 is N-(2-azido ethyl)-3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propionic acid amide., and compound 3 is 2-(2-(2-amino ethoxy) ethyoxyl) the ethyl carbamic acid tert-butyl ester;

(3) surface in situ bioactive molecule is fixed: first, at the fixing mercapto-polyglycol of C=C position of double bond, polyfunctional group surface modifying material is immersed into SH-PEG(40mM, 2ml) and DMPA(20mM, in mixed solution 2ml), 365nm irradiation under ultraviolet ray 5min, fully cleans with distilled water after taking-up; Then, at-NH ₂carboxylic bioactive molecule is fixed in position, and material is immersed into EDC(40mM, 1ml)/NHS(20mM, 1ml) fully reaction 8 hours in the mixed solution of/heparin (10mg/ml, 2ml), after taking-up, with distilled water, fully clean; Again, at-N ₃position is fixing to be immersed into polyfunctional group surface modifying material in the hirudin solution of 10mg/ml containing amino bioactive molecule, and 365nm ultra-vioket radiation 5 minutes, fully cleans with distilled water after taking-up; Finally ,-NHS group position is fixing to be immersed into the material of surface modification in the REDV solution of 10mg/ml containing amino bioactive molecule, and stirring at normal temperature reaction 8 hours, fully cleans rear dry with distilled water.

embodiment 10:according to following steps, material surface is processed

(1) surface active and self assembly: sample is processed with oxygen gas plasma after fully cleaning, discharge power is 50W, processing time is 10 minutes, sample after processing is immersed in the toluene solution of 5mM allyltrichlorosilane and reacts 6 hours, after fully cleaning, dries after taking out with toluene, acetone and distilled water;

(2) polyfunctional group chemical surface builds: first, by compound 1(20mM, 1ml) and DMPA(10mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorb after 1 minute and slowly dry up afterwards and fully contact with the material surface of two key modifications with argon, after 365nm irradiation under ultraviolet ray 1min, take off seal, with drying up with Ar after alcoholic solution ultrasonic cleaning, matrix is further immersed into EDC(10mM, 2ml)/NHS(5mM, in mixed solution 2ml), react 1 hour, distilled water dries up with Ar after cleaning; Then, by compound 2(20mM, 1ml) and DMPA(10mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorbs after 1 minute and dries up afterwards and fully contact with matrix surface with Ar, 365nm irradiation under ultraviolet ray 1min, take off seal, matrix is with drying up with Ar after alcoholic solution ultrasonic cleaning; Finally, by compound 3(5mM, alcoholic solution 1ml) is added drop-wise to PDMS seal surface, adsorb and with Ar, dry up after 1～3 minute, seal is fully contacted to 1min with matrix surface, carefully take off seal, matrix, with being immersed into room temperature reaction 10min in 90% trifluoroacetic acid (TFA) solution after alcoholic solution ultrasonic cleaning, dries up after cleaning; Compound 1 is 3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propanoic acid, compound 2 is N-(2-azido ethyl)-3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propionic acid amide., and compound 3 is 2-(2-(2-amino ethoxy) ethyoxyl) the ethyl carbamic acid tert-butyl ester;

(3) surface in situ bioactive molecule is fixed: first, at the fixing mercapto-polyglycol of C=C position of double bond, polyfunctional group surface modifying material is immersed into mercapto-polyglycol (20mM, 2ml) and DMPA(10mM, in mixed solution 2ml), 365nm irradiation under ultraviolet ray 3min, fully cleans with distilled water after taking-up; Then, at-NH ₂carboxylic bioactive molecule is fixed in position, and material is immersed into EDC(5mM, 1ml)/NHS(5mM, 1ml) fully reaction 2 hours in the mixed solution of/heparin (5mg/ml, 2ml), after taking-up, with distilled water, fully clean; Again, at-N ₃position is fixing to be immersed into polyfunctional group surface modifying material in the hirudin solution of 5mg/ml containing amino bioactive molecule, and 365nm ultra-vioket radiation 3 minutes, fully cleans with distilled water after taking-up; Finally ,-NHS group position is fixing to be immersed into the material of surface modification in the RGD solution of 5mg/ml containing amino bioactive molecule, and stirring at normal temperature reaction 2 hours, fully cleans rear dry with distilled water.

embodiment 11:according to following steps, material surface is processed

(1) surface active and self assembly: sample is processed with oxygen gas plasma after fully cleaning, discharge power is 200W, processing time is 20 minutes, sample after processing is immersed in the dichloromethane solution of 5mM allyltrichlorosilane and reacts 12 hours, after fully cleaning, dries after taking out with dichloromethane, acetone and distilled water;

(2) polyfunctional group chemical surface builds: first, by compound 1(20mM, 1ml) and DMPA(10mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorb after 2 minutes and slowly dry up afterwards and fully contact with the material surface of two key modifications with argon, after 365nm irradiation under ultraviolet ray 2min, take off seal, with drying up with Ar after alcoholic solution ultrasonic cleaning, matrix is further immersed into EDC(20mM, 2ml)/NHS(10mM, in mixed solution 2ml), react 2 hours, distilled water dries up with Ar after cleaning; Then, by compound 2(20mM, 1ml) and DMPA(10mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorbs after 2 minutes and dries up afterwards and fully contact with matrix surface with Ar, 365nm irradiation under ultraviolet ray 2min, take off seal, matrix is with drying up with Ar after alcoholic solution ultrasonic cleaning; Finally, by compound 3(5mM, alcoholic solution 1ml) is added drop-wise to PDMS seal surface, adsorb and with Ar, dry up after 2 minutes, seal is fully contacted to 2min with matrix surface, carefully take off seal, matrix, with being immersed into room temperature reaction 30min in 90% trifluoroacetic acid (TFA) solution after alcoholic solution ultrasonic cleaning, dries up after cleaning; Compound 1 is 3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propanoic acid, compound 2 is N-(2-azido ethyl)-3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propionic acid amide., and compound 3 is 2-(2-(2-amino ethoxy) ethyoxyl) the ethyl carbamic acid tert-butyl ester;

(3) surface in situ bioactive molecule is fixed: first, at the fixing mercapto-polyglycol of C=C position of double bond, polyfunctional group surface modifying material is immersed into mercapto-polyglycol (40mM, 2ml) and DMPA(20mM, in mixed solution 2ml), 365nm irradiation under ultraviolet ray 4min, fully cleans with distilled water after taking-up; Then, at-NH ₂carboxylic bioactive molecule is fixed in position, and material is immersed into EDC(20mM, 1ml)/NHS(10mM, 1ml)/bone morphogenetic protein 2 (BMP-2,10mg/ml, 2ml) mixed solution in fully reaction 4 hours, after taking-up, with distilled water, fully clean; Again, at-N ₃position is fixing to be immersed into polyfunctional group surface modifying material in the hirudin solution of 10mg/ml containing amino bioactive molecule, and 365nm ultra-vioket radiation 4 minutes, fully cleans with distilled water after taking-up; Finally ,-NHS group position is fixing to be immersed into the material of surface modification in the fibronectin solution of 10mg/ml containing amino bioactive molecule, and stirring at normal temperature reaction 4 hours, fully cleans rear dry with distilled water.

embodiment 12:according to following steps, material surface is processed

(1) surface active and self assembly: sample is processed with oxygen gas plasma after fully cleaning, discharge power is 300W, processing time is 30 minutes, sample after processing is immersed in the dimethyl formamide solution of 5mM allyltrimethoxysilanis and reacts 12 hours, after fully cleaning, dries after taking out with dimethyl formamide, ethanol and distilled water;

(2) polyfunctional group chemical surface builds: first, by compound 1(50mM, 1ml) and DMPA(25mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorb after 3 minutes and slowly dry up afterwards and fully contact with the material surface of two key modifications with argon, after 365nm irradiation under ultraviolet ray 3min, take off seal, with drying up with Ar after alcoholic solution ultrasonic cleaning, matrix is further immersed into EDC(40mM, 2ml)/NHS(20mM, in mixed solution 2ml), react 4 hours, distilled water dries up with Ar after cleaning; Then, by compound 2(50mM, 1ml) and DMPA(25mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorbs after 3 minutes and dries up afterwards and fully contact with matrix surface with Ar, 365nm irradiation under ultraviolet ray 3min, take off seal, matrix is with drying up with Ar after alcoholic solution ultrasonic cleaning; Finally, by compound 3(10mM, alcoholic solution 1ml) is added drop-wise to PDMS seal surface, adsorb and with Ar, dry up after 3 minutes, seal is fully contacted to 3min with matrix surface, carefully take off seal, matrix, with being immersed into room temperature reaction 60min in 90% trifluoroacetic acid (TFA) solution after alcoholic solution ultrasonic cleaning, dries up after cleaning; Compound 1 is 3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propanoic acid, compound 2 is N-(2-azido ethyl)-3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propionic acid amide., and compound 3 is 2-(2-(2-amino ethoxy) ethyoxyl) the ethyl carbamic acid tert-butyl ester;

(3) surface in situ bioactive molecule is fixed: first, at the fixing mercapto-polyglycol of C=C position of double bond, polyfunctional group surface modifying material is immersed into mercapto-polyglycol (40mM, 2ml) and DMPA(20mM, in mixed solution 2ml), 365nm irradiation under ultraviolet ray 5min, fully cleans with distilled water after taking-up; Then, at-NH ₂carboxylic bioactive molecule is fixed in position, and material is immersed into EDC(40mM, 1ml)/NHS(20mM, 1ml) fully reaction 8 hours in the mixed solution of/heparin (10mg/ml, 2ml), after taking-up, with distilled water, fully clean; Again, at-N ₃position is fixing to be immersed into polyfunctional group surface modifying material in the hirudin solution of 10mg/ml containing amino bioactive molecule, and 365nm ultra-vioket radiation 5 minutes, fully cleans with distilled water after taking-up; Finally ,-NHS group position is fixing to be immersed into the material of surface modification in the BMP-2 solution of 10mg/ml containing amino bioactive molecule, and stirring at normal temperature reaction 8 hours, fully cleans rear dry with distilled water.

embodiment 13:according to following steps, material surface is processed

(1) surface active and self assembly: after sample fully cleans, with oxygen gas plasma processing, (discharge power is 50W, processing time is 10 minutes), sample after processing is immersed in the toluene solution of 5mM allyltrichlorosilane and reacts 6 hours, after fully cleaning, dries after taking out with toluene, acetone and distilled water;

(2) polyfunctional group chemical surface builds: first, by compound 1(50mM, 1ml) and DMPA(25mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorb after 1 minute and slowly dry up afterwards and fully contact with the material surface of two key modifications with argon, after 365nm irradiation under ultraviolet ray 1min, take off seal, with drying up with Ar after alcoholic solution ultrasonic cleaning, matrix is further immersed into EDC(10mM, 2ml)/NHS(5mM, in mixed solution 2ml), react 1 hour, distilled water dries up with Ar after cleaning; Then, by compound 2(50mM, 1ml) and DMPA(25mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorbs after 1 minute and dries up afterwards and fully contact with matrix surface with Ar, 365nm irradiation under ultraviolet ray 1min, take off seal, matrix is with drying up with Ar after alcoholic solution ultrasonic cleaning; Finally, by compound 3(10mM, alcoholic solution 1ml) is added drop-wise to PDMS seal surface, adsorb and with Ar, dry up after 1 minute, seal is fully contacted to 1min with matrix surface, carefully take off seal, matrix, with being immersed into room temperature reaction 10min in 90% trifluoroacetic acid (TFA) solution after alcoholic solution ultrasonic cleaning, dries up after cleaning; Compound 1 is 3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propanoic acid, compound 2 is N-(2-azido ethyl)-3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propionic acid amide., and compound 3 is 2-(2-(2-amino ethoxy) ethyoxyl) the ethyl carbamic acid tert-butyl ester;

(3) surface in situ bioactive molecule is fixed: first, at the fixing mercapto-polyglycol of C=C position of double bond, polyfunctional group surface modifying material is immersed into SH-PEG(2mM, 2ml) and DMPA(2mM, in mixed solution 2ml), 365nm irradiation under ultraviolet ray 3min, fully cleans with distilled water after taking-up; Then, at-NH ₂carboxylic bioactive molecule is fixed in position, and material is immersed into EDC(5mM, 1ml)/NHS(5mM, 1ml) fully reaction 2 hours in the mixed solution of/albumin (2mg/ml, 2ml), after taking-up, with distilled water, fully clean; Again, at-N ₃position is fixing to be immersed into polyfunctional group surface modifying material in the hirudin solution of 1mg/ml containing amino bioactive molecule, and 365nm ultra-vioket radiation 3 minutes, fully cleans with distilled water after taking-up; Finally ,-NHS group position is fixing to be immersed into the material of surface modification in the REDV solution of 1mg/ml containing amino bioactive molecule, and stirring at normal temperature reaction 2 hours, fully cleans rear dry with distilled water.

embodiment 14:according to following steps, material surface is processed

(1) surface active and self assembly: after sample fully cleans, with oxygen gas plasma processing, (discharge power is 200W, processing time is 20 minutes), sample after processing is immersed in the alcoholic solution of 5mM allyltrimethoxysilanis and reacts 12 hours, after fully cleaning, dries after taking out with ethanol and distilled water;

(2) polyfunctional group chemical surface builds: first, by compound 1(80mM, 1ml) and DMPA(40mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorb after 2 minutes and slowly dry up afterwards and fully contact with the material surface of two key modifications with argon, after 365nm irradiation under ultraviolet ray 2min, take off seal, with drying up with Ar after alcoholic solution ultrasonic cleaning, matrix is further immersed into EDC(20mM, 2ml)/NHS(10mM, in mixed solution 2ml), react 2 hours, distilled water dries up with Ar after cleaning; Then, by compound 2(80mM, 1ml) and DMPA(40mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorbs after 2 minutes and dries up afterwards and fully contact with matrix surface with Ar, 365nm irradiation under ultraviolet ray 2min, take off seal, matrix is with drying up with Ar after alcoholic solution ultrasonic cleaning; Finally, by compound 3(20mM, alcoholic solution 1ml) is added drop-wise to PDMS seal surface, adsorb and with Ar, dry up after 2 minutes, seal is fully contacted to 2min with matrix surface, carefully take off seal, matrix, with being immersed into room temperature reaction 30min in 90% trifluoroacetic acid (TFA) solution after alcoholic solution ultrasonic cleaning, dries up after cleaning; Compound 1 is 3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propanoic acid, compound 2 is N-(2-azido ethyl)-3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propionic acid amide., and compound 3 is 2-(2-(2-amino ethoxy) ethyoxyl) the ethyl carbamic acid tert-butyl ester;

(3) surface in situ bioactive molecule is fixed: first, at the fixing mercapto-polyglycol of C=C position of double bond, polyfunctional group surface modifying material is immersed into mercapto-polyglycol (2mM, 2ml) and DMPA(2mM, in mixed solution 2ml), 365nm irradiation under ultraviolet ray 4min, fully cleans with distilled water after taking-up; Then, at-NH ₂carboxylic bioactive molecule is fixed in position, and material is immersed into EDC(20mM, 1ml)/NHS(10mM, 1ml) fully reaction 4 hours in the mixed solution of/heparin (2mg/ml, 2ml), after taking-up, with distilled water, fully clean; Again, at-N ₃position is fixing to be immersed into polyfunctional group surface modifying material in the BMP-2 solution of 1mg/ml containing amino bioactive molecule, and 365nm ultra-vioket radiation 4 minutes, fully cleans with distilled water after taking-up; Finally ,-NHS group position is fixing to be immersed into the material of surface modification in the REDV solution of 1mg/ml containing amino bioactive molecule, and stirring at normal temperature reaction 4 hours, fully cleans rear dry with distilled water.

embodiment 15:according to following steps, material surface is processed

(1) surface active and self assembly: sample is processed with oxygen gas plasma after fully cleaning, discharge power is 300W, processing time is 30 minutes, sample after processing is immersed in the alcoholic solution of 5mM allyltriethoxysilane and reacts 24 hours, after fully cleaning, dries after taking out with ethanol and distilled water;

(2) polyfunctional group chemical surface builds: first, by compound 1(80mM, 1ml) and DMPA(40mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorb after 3 minutes and slowly dry up afterwards and fully contact with the material surface of two key modifications with argon, after 365nm irradiation under ultraviolet ray 3min, take off seal, with drying up with Ar after alcoholic solution ultrasonic cleaning, matrix is further immersed into EDC(40mM, 2ml)/NHS(20mM, in mixed solution 2ml), react 4 hours, distilled water dries up with Ar after cleaning; Then, by compound 2(80mM, 1ml) and DMPA(40mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorbs after 3 minutes and dries up afterwards and fully contact with matrix surface with Ar, 365nm irradiation under ultraviolet ray 3min, take off seal, matrix is with drying up with Ar after alcoholic solution ultrasonic cleaning; Finally, by compound 3(20mM, alcoholic solution 1ml) is added drop-wise to PDMS seal surface, adsorb and with Ar, dry up after 3 minutes, seal is fully contacted to 3min with matrix surface, carefully take off seal, matrix, with being immersed into room temperature reaction 60min in 90% trifluoroacetic acid (TFA) solution after alcoholic solution ultrasonic cleaning, dries up after cleaning; Compound 1 is 3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propanoic acid, compound 2 is N-(2-azido ethyl)-3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propionic acid amide., and compound 3 is 2-(2-(2-amino ethoxy) ethyoxyl) the ethyl carbamic acid tert-butyl ester;

(3) surface in situ bioactive molecule is fixed: first, at the fixing mercapto-polyglycol of C=C position of double bond, polyfunctional group surface modifying material is immersed into mercapto-polyglycol (20mM, 2ml) and DMPA(10mM, in mixed solution 2ml), 365nm irradiation under ultraviolet ray 5min, fully cleans with distilled water after taking-up; Then, at-NH ₂carboxylic bioactive molecule is fixed in position, and material is immersed into EDC(40mM, 1ml)/NHS(20mM, 1ml) fully reaction 8 hours in the mixed solution of/heparin (5mg/ml, 2ml), after taking-up, with distilled water, fully clean; Again, at-N ₃position is fixing to be immersed into polyfunctional group surface modifying material in the hirudin solution of 5mg/ml containing amino bioactive molecule, and 365nm ultra-vioket radiation 5 minutes, fully cleans with distilled water after taking-up; Finally ,-NHS group position is fixing to be immersed into the material of surface modification in the BMP-2 solution of 5mg/ml containing amino bioactive molecule, and stirring at normal temperature reaction 8 hours, fully cleans rear dry with distilled water.

embodiment 16:according to following steps, material surface is processed

(1) surface active and self assembly: sample is processed with oxygen gas plasma after fully cleaning, discharge power is 50W, processing time is 10 minutes, sample after processing is immersed in the toluene solution of 50mM allyltrichlorosilane and reacts 6 hours, after fully cleaning, dries after taking out with toluene, acetone and distilled water;

(2) polyfunctional group chemical surface builds: first, by compound 1(20mM, 1ml) and DMPA(10mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorb after 1 minute and slowly dry up afterwards and fully contact with the material surface of two key modifications with argon, after 365nm irradiation under ultraviolet ray 1min, take off seal, with drying up with Ar after alcoholic solution ultrasonic cleaning, matrix is further immersed into EDC(10mM, 2ml)/NHS(5mM, in mixed solution 2ml), react 1 hour, distilled water dries up with Ar after cleaning; Then, by compound 2(20mM, 1ml) and DMPA(10mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorbs after 1 minute and dries up afterwards and fully contact with matrix surface with Ar, 365nm irradiation under ultraviolet ray 1min, take off seal, matrix is with drying up with Ar after alcoholic solution ultrasonic cleaning; Finally, by compound 3(5mM, alcoholic solution 1ml) is added drop-wise to PDMS seal surface, adsorb and with Ar, dry up after 1 minute, seal is fully contacted to 1min with matrix surface, carefully take off seal, matrix, with being immersed into room temperature reaction 10min in 90% trifluoroacetic acid (TFA) solution after alcoholic solution ultrasonic cleaning, dries up after cleaning; Compound 1 is 3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propanoic acid, compound 2 is N-(2-azido ethyl)-3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propionic acid amide., and compound 3 is 2-(2-(2-amino ethoxy) ethyoxyl) the ethyl carbamic acid tert-butyl ester;

(3) surface in situ bioactive molecule is fixed: first, at the fixing mercapto-polyglycol of C=C position of double bond, polyfunctional group surface modifying material is immersed into mercapto-polyglycol (20mM, 2ml) and DMPA(10mM, in mixed solution 2ml), 365nm irradiation under ultraviolet ray 3min, fully cleans with distilled water after taking-up; Then, at-NH ₂carboxylic bioactive molecule is fixed in position, and material is immersed into EDC(5mM, 1ml)/NHS(5mM, 1ml) fully reaction 2 hours in the mixed solution of/heparin (5mg/ml, 2ml), after taking-up, with distilled water, fully clean; Again, at-N ₃position is fixing to be immersed into polyfunctional group surface modifying material in the hirudin solution of 5mg/ml containing amino bioactive molecule, and 365nm ultra-vioket radiation 3 minutes, fully cleans with distilled water after taking-up; Finally ,-NHS group position is fixing to be immersed into the material of surface modification in the albumin solution of 5mg/ml containing amino bioactive molecule, and stirring at normal temperature reaction 2 hours, fully cleans rear dry with distilled water.

embodiment 17:according to following steps, material surface is processed

(1) surface active and self assembly: sample is processed with oxygen gas plasma after fully cleaning, discharge power is 200W, processing time is 20 minutes, sample after processing is immersed in the dimethyl formamide solution of 50mM allyltrimethoxysilanis and reacts 12 hours, after fully cleaning, dries after taking out with dimethyl formamide, ethanol and distilled water;

(3) surface in situ bioactive molecule is fixed: first, at the fixing mercapto-polyglycol of C=C position of double bond, polyfunctional group surface modifying material is immersed into mercapto-polyglycol (40mM, 2ml) and DMPA(20mM, in mixed solution 2ml), 365nm irradiation under ultraviolet ray 4min, fully cleans with distilled water after taking-up; Then, at-NH ₂carboxylic bioactive molecule is fixed in position, and material is immersed into EDC(20mM, 1ml)/NHS(10mM, 1ml)/REDV(10mg/ml, 2ml) and mixed solution in fully reaction 4 hours, after taking-up, with distilled water, fully clean; Again, at-N ₃position is fixing to be immersed into polyfunctional group surface modifying material in the hirudin solution of 10mg/ml containing amino bioactive molecule, and 365nm ultra-vioket radiation 4 minutes, fully cleans with distilled water after taking-up; Finally ,-NHS group position is fixing to be immersed into the material of surface modification in the VEGF solution of 10mg/ml containing amino bioactive molecule, and stirring at normal temperature reaction 4 hours, fully cleans rear dry with distilled water.

embodiment 18:according to following steps, material surface is processed

(1) surface active and self assembly: sample is processed with oxygen gas plasma after fully cleaning, discharge power is 300W, processing time is 30 minutes, sample after processing is immersed in the toluene solution of 50mM allyltrichlorosilane and reacts 24 hours, after fully cleaning, dries after taking out with toluene, acetone and distilled water;

(2) polyfunctional group chemical surface builds: first, by compound 1(50mM, 1ml) and DMPA(25mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorb after 3 minutes and slowly dry up afterwards and fully contact with the material surface of two key modifications with argon, after 365nm irradiation under ultraviolet ray 3min, take off seal, with drying up with Ar after alcoholic solution ultrasonic cleaning, matrix is further immersed into EDC(40mM, 2ml)/NHS(20mM, in mixed solution 2ml), react 4 hours, distilled water dries up with Ar after cleaning; Then, by compound 2(50mM, 1ml) and DMPA(25mM, alcohol mixed solution 2ml) is added drop-wise to seal surface, adsorbs after 3 minutes and dries up afterwards and fully contact with matrix surface with Ar, 365nm irradiation under ultraviolet ray 3min, take off seal, matrix is with drying up with Ar after alcoholic solution ultrasonic cleaning; Finally, by compound 3(10mM, alcoholic solution 1ml) is added drop-wise to PDMS seal surface, adsorb and with Ar, dry up after 3 minutes, seal is fully contacted to 3min with matrix surface, carefully take off seal, matrix, with being immersed into room temperature reaction 60min in 90% trifluoroacetic acid (TFA) solution after alcoholic solution ultrasonic cleaning, dries up after cleaning; Compound 1 is 3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propanoic acid, compound 2 is N-(2-azido ethyl)-3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propionic acid amide., and compound 3 is 2-(2-(2-amino ethoxy) ethyoxyl) the ethyl carbamic acid tert-butyl ester;

(3) surface in situ bioactive molecule is fixed: first, at the fixing mercapto-polyglycol of C=C position of double bond, polyfunctional group surface modifying material is immersed into mercapto-polyglycol (40mM, 2ml) and DMPA(20mM, in mixed solution 2ml), 365nm irradiation under ultraviolet ray 5min, fully cleans with distilled water after taking-up; Then, at-NH ₂carboxylic bioactive molecule is fixed in position, and material is immersed into EDC(40mM, 1ml)/NHS(20mM, 1ml) fully reaction 8 hours in the mixed solution of/heparin (10mg/ml, 2ml), after taking-up, with distilled water, fully clean; Again, at-N ₃position is fixing to be immersed into polyfunctional group surface modifying material in the hirudin solution of 10mg/ml containing amino bioactive molecule, and 365nm ultra-vioket radiation 5 minutes, fully cleans with distilled water after taking-up; Finally ,-NHS group position is fixing to be immersed into the material of surface modification in the albumin solution of 10mg/ml containing amino bioactive molecule, and stirring at normal temperature reaction 8 hours, fully cleans rear dry with distilled water.

embodiment 19:according to following steps, material surface is processed

(1) surface active and self assembly: sample is processed with oxygen gas plasma after fully cleaning, discharge power is 50W, processing time is 10 minutes, sample after processing is immersed in the dimethyl sulphoxide solution of 50mM allyltrimethoxysilanis and reacts 6 hours, after fully cleaning, dries after taking out with dimethyl sulfoxide, ethanol and distilled water;

(3) surface in situ bioactive molecule is fixed: first, at the fixing mercapto-polyglycol of C=C position of double bond, polyfunctional group surface modifying material is immersed into mercapto-polyglycol (2mM, 2ml) and DMPA(2mM, in mixed solution 2ml), 365nm irradiation under ultraviolet ray 3min, fully cleans with distilled water after taking-up; Then, at-NH ₂carboxylic bioactive molecule is fixed in position, and material is immersed into EDC(5mM, 1ml)/NHS(5mM, 1ml)/VEGF(2mg/ml, 2ml) and mixed solution in fully reaction 2 hours, after taking-up, with distilled water, fully clean; Again, at-N ₃position is fixing to be immersed into polyfunctional group surface modifying material in the hirudin solution of 1mg/ml containing amino bioactive molecule, and 365nm ultra-vioket radiation 3 minutes, fully cleans with distilled water after taking-up; Finally ,-NHS group position is fixing to be immersed into the material of surface modification in the albumin solution of 1mg/ml containing amino bioactive molecule, and stirring at normal temperature reaction 2 hours, fully cleans rear dry with distilled water.

embodiment 20:according to following steps, material surface is processed

(1) surface active and self assembly: sample is processed with oxygen gas plasma after fully cleaning, discharge power is 200W, processing time is 20 minutes, sample after processing is immersed in the alcoholic solution of 50mM allyltrimethoxysilanis and reacts 12 hours, after fully cleaning, dries after taking out with ethanol and distilled water;

(3) surface in situ bioactive molecule is fixed: first, at the fixing mercapto-polyglycol of C=C position of double bond, polyfunctional group surface modifying material is immersed into SH-PEG(2mM, 2ml) and DMPA(2mM, in mixed solution 2ml), 365nm irradiation under ultraviolet ray 4min, fully cleans with distilled water after taking-up; Then, at-NH ₂carboxylic bioactive molecule is fixed in position, and material is immersed into EDC(20mM, 1ml)/NHS(10mM, 1ml) fully reaction 4 hours in the mixed solution of/heparin (2mg/ml, 2ml), after taking-up, with distilled water, fully clean; Again, at-N ₃position is fixing to be immersed into polyfunctional group surface modifying material in the hirudin solution of 1mg/ml containing amino bioactive molecule, and 365nm ultra-vioket radiation 4 minutes, fully cleans with distilled water after taking-up; Finally ,-NHS group position is fixing to be immersed into the material of surface modification in the REDV solution of 1mg/ml containing amino bioactive molecule, and stirring at normal temperature reaction 4 hours, fully cleans rear dry with distilled water.

embodiment 21:according to following steps, material surface is processed

(1) surface active and self assembly: sample is processed with oxygen gas plasma after fully cleaning, discharge power is 300W, processing time is 30 minutes, sample after processing is immersed in the alcoholic solution of 50mM allyltriethoxysilane and reacts 24 hours, after fully cleaning, dries after taking out with ethanol and distilled water;

(3) surface in situ bioactive molecule is fixed: first, at the fixing mercapto-polyglycol of C=C position of double bond, polyfunctional group surface modifying material is immersed into SH-PEG(20mM, 2ml) and DMPA(10mM, in mixed solution 2ml), 365nm irradiation under ultraviolet ray 5min, fully cleans with distilled water after taking-up; Then, at-NH ₂carboxylic bioactive molecule is fixed in position, and material is immersed into EDC(40mM, 1ml)/NHS(20mM, 1ml) fully reaction 8 hours in the mixed solution of/heparin (5mg/ml, 2ml), after taking-up, with distilled water, fully clean; Again, at-N ₃position is fixing to be immersed into polyfunctional group surface modifying material in the hirudin solution of 5mg/ml containing amino bioactive molecule, and 365nm ultra-vioket radiation 5 minutes, fully cleans with distilled water after taking-up; Finally ,-NHS group position is fixing to be immersed into the material of surface modification in the VEGF solution of 5mg/ml containing amino bioactive molecule, and stirring at normal temperature reaction 8 hours, fully cleans rear dry with distilled water.

embodiment 22:according to following steps, material surface is processed

(1) surface active and self assembly: sample is processed with oxygen gas plasma after fully cleaning, discharge power is 50W, processing time is 10 minutes, sample after processing is immersed in the dichloromethane solution of 100mM allyltrichlorosilane and reacts 6 hours, after fully cleaning, dries after taking out with dichloromethane, acetone and distilled water;

(2) polyfunctional group chemical surface builds: first, by compound 1(20mM, 1ml) and DMPA(10mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorb after 1 minute and slowly dry up afterwards and fully contact with the material surface of two key modifications with argon, after 365nm irradiation under ultraviolet ray 1min, take off seal, with drying up with Ar after alcoholic solution ultrasonic cleaning, matrix is further immersed into EDC(10mM, 2ml)/NHS(5mM, in mixed solution 2ml), react 1 hour, distilled water dries up with Ar after cleaning; Then, by compound 2(20mM, 1ml) and DMPA(10mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorbs after 1 minute and dries up afterwards and fully contact with matrix surface with Ar, 365nm irradiation under ultraviolet ray 1min, take off seal, matrix is with drying up with Ar after alcoholic solution ultrasonic cleaning; Finally, by compound 3(5mM, alcoholic solution 1ml) is added drop-wise to PDMS seal surface, adsorb and with Ar, dry up after 3 minutes, seal is fully contacted to 1min with matrix surface, carefully take off seal, matrix, with being immersed into room temperature reaction 10min in 90% trifluoroacetic acid (TFA) solution after alcoholic solution ultrasonic cleaning, dries up after cleaning; Compound 1 is 3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propanoic acid, compound 2 is N-(2-azido ethyl)-3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propionic acid amide., and compound 3 is 2-(2-(2-amino ethoxy) ethyoxyl) the ethyl carbamic acid tert-butyl ester;

(3) surface in situ bioactive molecule is fixed: first, at the fixing mercapto-polyglycol of C=C position of double bond, polyfunctional group surface modifying material is immersed into mercapto-polyglycol (20mM, 2ml) and DMPA(10mM, in mixed solution 2ml), 365nm irradiation under ultraviolet ray 3min, fully cleans with distilled water after taking-up; Then, at-NH ₂carboxylic bioactive molecule is fixed in position, and material is immersed into EDC(5mM, 1ml)/NHS(20mM, 1ml)/VEGF(5mg/ml, 2ml) and mixed solution in fully reaction 2 hours, after taking-up, with distilled water, fully clean; Again, at-N ₃position is fixing to be immersed into polyfunctional group surface modifying material in the hirudin solution of 5mg/ml containing amino bioactive molecule, and 365nm ultra-vioket radiation 3 minutes, fully cleans with distilled water after taking-up; Finally ,-NHS group position is fixing to be immersed into the material of surface modification in the albumin solution of 5mg/ml containing amino bioactive molecule, and stirring at normal temperature reaction 2 hours, fully cleans rear dry with distilled water.

embodiment 23:according to following steps, material surface is processed

(1) surface active and self assembly: sample is processed with oxygen gas plasma after fully cleaning, discharge power is 200W, processing time is 20 minutes, sample after processing is immersed in the dimethyl formamide solution of 100mM allyltrimethoxysilanis and reacts 12 hours, after fully cleaning, dries after taking out with dimethyl formamide, ethanol and distilled water;

(3) surface in situ bioactive molecule is fixed: first, at the fixing mercapto-polyglycol of C=C position of double bond, polyfunctional group surface modifying material is immersed into mercapto-polyglycol (40mM, 2ml) and DMPA(20mM, in mixed solution 2ml), 365nm irradiation under ultraviolet ray 4min, fully cleans with distilled water after taking-up; Then, at-NH ₂carboxylic bioactive molecule is fixed in position, and material is immersed into EDC(20mM, 1ml)/NHS(10mM, 1ml) fully reaction 4 hours in the mixed solution of/heparin (10mg/ml, 2ml), after taking-up, with distilled water, fully clean; Again, at-N ₃position is fixing to be immersed into polyfunctional group surface modifying material in the hirudin solution of 10mg/ml containing amino bioactive molecule, and 365nm ultra-vioket radiation 4 minutes, fully cleans with distilled water after taking-up; Finally ,-NHS group position is fixing to be immersed into the material of surface modification in the REDV solution of 10mg/ml containing amino bioactive molecule, and stirring at normal temperature reaction 4 hours, fully cleans rear dry with distilled water.

embodiment 24:according to following steps, material surface is processed

(1) surface active and self assembly: sample is processed with oxygen gas plasma after fully cleaning, discharge power is 300W, processing time is 30 minutes, sample after processing is immersed in the alcoholic solution of 100mM allyltrimethoxysilanis and reacts 24 hours, after fully cleaning, dries after taking out with ethanol and distilled water;

(3) surface in situ bioactive molecule is fixed: first, at the fixing mercapto-polyglycol of C=C position of double bond, polyfunctional group surface modifying material is immersed into mercapto-polyglycol (40mM, 2ml) and DMPA(20mM, in mixed solution 2ml), 365nm irradiation under ultraviolet ray 5min, fully cleans with distilled water after taking-up; Then, at-NH ₂carboxylic bioactive molecule is fixed in position, and material is immersed into EDC(40mM, 1ml)/NHS(20mM, 1ml) fully reaction 8 hours in the mixed solution of/heparin (10mg/ml, 2ml), after taking-up, with distilled water, fully clean; Again, at-N ₃position is fixing to be immersed into polyfunctional group surface modifying material in the hirudin solution of 10mg/ml containing amino bioactive molecule, and 365nm ultra-vioket radiation 5 minutes, fully cleans with distilled water after taking-up; Finally ,-NHS group position is fixing to be immersed into the material of surface modification in the VEGF solution of 10mg/ml containing amino bioactive molecule, and stirring at normal temperature reaction 8 hours, fully cleans rear dry with distilled water.

embodiment 25:according to following steps, material surface is processed

(1) surface active and self assembly: sample is processed with oxygen gas plasma after fully cleaning, discharge power is 50W, processing time is 10 minutes, sample after processing is immersed in the toluene solution of 100mM allyltriethoxysilane and reacts 6 hours, after fully cleaning, dries after taking out with toluene, acetone and distilled water;

(3) surface in situ bioactive molecule is fixed: first, at the fixing mercapto-polyglycol of C=C position of double bond, polyfunctional group surface modifying material is immersed into mercapto-polyglycol (2mM, 2ml) and DMPA(2mM, in mixed solution 2ml), 365nm irradiation under ultraviolet ray 3min, fully cleans with distilled water after taking-up; Then, at-NH ₂carboxylic bioactive molecule is fixed in position, and material is immersed into EDC(5mM, 1ml)/NHS(5mM, 1ml) fully reaction 2 hours in the mixed solution of/albumin (2mg/ml, 2ml), after taking-up, with distilled water, fully clean; Again, at-N ₃position is fixing to be immersed into polyfunctional group surface modifying material in the VEGF solution of 1mg/ml containing amino bioactive molecule, and 365nm ultra-vioket radiation 3 minutes, fully cleans with distilled water after taking-up; Finally ,-NHS group position is fixing to be immersed into the material of surface modification in the RGD solution of 1mg/ml containing amino bioactive molecule, and stirring at normal temperature reaction 2 hours, fully cleans rear dry with distilled water.

embodiment 26:according to following steps, material surface is processed

(1) surface active and self assembly: sample is processed with oxygen gas plasma after fully cleaning, discharge power is 200W, processing time is 20 minutes, sample after processing is immersed in the toluene solution of 100mM allyltrichlorosilane and reacts 12 hours, after fully cleaning, dries after taking out with toluene, acetone and distilled water;

(2) polyfunctional group chemical surface builds: first, by compound 1(80mM, 1ml) and DMPA(40mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorb after 2 minutes and slowly dry up afterwards and fully contact with the material surface of two key modifications with argon, after 365nm irradiation under ultraviolet ray 2min, take off seal, with drying up with Ar after alcoholic solution ultrasonic cleaning, matrix is further immersed into EDC(20mM, 2ml)/NHS(10mM, in mixed solution 2ml), react 2 hours, distilled water dries up with Ar after cleaning; Then, by compound 2(50mM, 1ml) and DMPA(25mM, alcohol mixed solution 1ml) is added drop-wise to seal surface, adsorbs after 2 minutes and dries up afterwards and fully contact with matrix surface with Ar, 365nm irradiation under ultraviolet ray 2min, take off seal, matrix is with drying up with Ar after alcoholic solution ultrasonic cleaning; Finally, by compound 3(20mM, alcoholic solution 1ml) is added drop-wise to PDMS seal surface, adsorb and with Ar, dry up after 2 minutes, seal is fully contacted to 2min with matrix surface, carefully take off seal, matrix, with being immersed into room temperature reaction 30min in 90% trifluoroacetic acid (TFA) solution after alcoholic solution ultrasonic cleaning, dries up after cleaning; Compound 1 is 3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propanoic acid, compound 2 is N-(2-azido ethyl)-3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propionic acid amide., and compound 3 is 2-(2-(2-amino ethoxy) ethyoxyl) the ethyl carbamic acid tert-butyl ester;

(3) surface in situ bioactive molecule is fixed: first, at the fixing mercapto-polyglycol of C=C position of double bond, polyfunctional group surface modifying material is immersed into mercapto-polyglycol (20mM, 2ml) and DMPA(10mM, in mixed solution 2ml), 365nm irradiation under ultraviolet ray 4min, fully cleans with distilled water after taking-up; Then, at-NH ₂carboxylic bioactive molecule is fixed in position, and material is immersed into EDC(20mM, 1ml)/NHS(10mM, 1ml) fully reaction 4 hours in the mixed solution of/heparin (5mg/ml, 2ml), after taking-up, with distilled water, fully clean; Again, at-N ₃position is fixing to be immersed into polyfunctional group surface modifying material in the hirudin solution of 5mg/ml containing amino bioactive molecule, and 365nm ultra-vioket radiation 4 minutes, fully cleans with distilled water after taking-up; Finally ,-NHS group position is fixing to be immersed into the material of surface modification in the REDV solution of 5mg/ml containing amino bioactive molecule, and stirring at normal temperature reaction 4 hours, fully cleans rear dry with distilled water.

embodiment 27:according to following steps, material surface is processed

(3) surface in situ bioactive molecule is fixed: first, at the fixing mercapto-polyglycol of C=C position of double bond, polyfunctional group surface modifying material is immersed into mercapto-polyglycol (2mM, 2ml) and DMPA(2mM, in mixed solution 2ml), 365nm irradiation under ultraviolet ray 5min, fully cleans with distilled water after taking-up; Then, at-NH ₂carboxylic bioactive molecule is fixed in position, and material is immersed into EDC(40mM, 1ml)/NHS(20mM, 1ml)/VEGF(2mg/ml, 2ml) and mixed solution in fully reaction 8 hours, after taking-up, with distilled water, fully clean; Again, at-N ₃position is fixing to be immersed into material in the hirudin solution of 1mg/ml containing amino bioactive molecule, and 365nm ultra-vioket radiation 5 minutes, fully cleans with distilled water after taking-up; Finally ,-NHS group position is fixing to be immersed into the material of surface modification in the albumin solution of 1mg/ml containing amino bioactive molecule, and stirring at normal temperature reaction 8 hours, fully cleans rear dry with distilled water.

Claims

1. the surface modifying method of biomaterial, it is characterized in that: first material surface is carried out carrying out surface self-organization after oxygen gas plasma activation processing, then adopt Microcontact printing alternately fixedly to there is successively the chemical molecular of different functional groups, and then successively the bioactive molecule with difference in functionality is fixed on to material surface, obtain having the biomaterial of multifunctional bio-active.

2. the surface modifying method of biomaterial according to claim 1, is characterized in that: the discharge mode of described oxygen gas plasma activation processing is radio-frequency discharge mode, and discharge power is 50W～300W, and the processing time is 10～30 minutes.

3. the surface modifying method of biomaterial according to claim 1, it is characterized in that: described surface self-organization is surface self-organization thiazolinyl silane, thiazolinyl silane is allyltrimethoxysilanis, allyltriethoxysilane or allyltrichlorosilane, the self-assembling reaction time is 6～24 hours, solution concentration is 5mM～100mM, and solvent is a kind of in dichloromethane, ethanol, toluene, dimethyl sulfoxide, dimethyl formamide.

4. the surface modifying method of biomaterial according to claim 1, it is characterized in that: described Microcontact printing is to adopt ribbon micrographics seal on the matrix surface of self assembly thiazolinyl silane, to replace successively fixed compound 1, compound 2 and compound 3, compound 1 is the chemical molecular (3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propanoic acid and analog thereof) that two ends are respectively sulfydryl and carboxyl, compound 2 is chemical moleculars (N-(2-azido ethyl)-3-(2-(2-sulfydryl ethyoxyl) ethyoxyl) propionic acid amide. and analog thereof) that two ends are respectively sulfydryl and azido group, compound 3 is chemical moleculars (2-(2-(2-amino ethoxy) ethyoxyl) the ethyl carbamic acid tert-butyl ester and analog thereof) that two ends are respectively amino and t-butyl carbamate base.

5. the surface modifying method of biomaterial according to claim 4, it is characterized in that: described micro-contact printing fixed compound 1 is by compound 1(20～80mM) and 2, 2-dimethoxy-phenyl 1-Phenylethanone. (DMPA, 10～40mM) alcohol mixed solution is added drop-wise to seal surface, fully absorption slowly dried up with argon after 1～3 minute, again seal is fully contacted with material modified surface, after the irradiation under ultraviolet ray 1～3min of room temperature with 365nm, take off seal, matrix is with drying up with Ar after alcoholic solution ultrasonic cleaning, further be immersed into 1-(3-dimethylaminopropyl)-3-ethyl carbodiimide (EDC, 10～40mM)/N-maloyl imines (NHS, 5～20mM) in mixed solution, react 1～4 hour, distilled water dries up with Ar after cleaning.

6. the surface modifying method of biomaterial according to claim 4, it is characterized in that: described micro-contact printing fixed compound 2 is by compound 2(20～80mM) and DMPA(10～40mM) alcohol mixed solution be added drop-wise to seal surface, fully absorption dried up with Ar after 1～3 minute, again seal is fully contacted with material modified surface, under room temperature, use irradiation under ultraviolet ray 1～3min of 365nm, carefully take off seal, matrix is with drying up with Ar after alcoholic solution ultrasonic cleaning.

7. the surface modifying method of biomaterial according to claim 4, it is characterized in that: described micro-contact printing fixed compound 3 is by compound 3(5～20mM) alcoholic solution be added drop-wise to PDMS seal surface, fully absorption dried up with Ar after 1～3 minute, again seal is fully contacted to 1～3min with material modified surface, carefully take off seal, matrix is room temperature reaction 10～60min in 90% trifluoroacetic acid (TFA) solution with being immersed into mass concentration after alcoholic solution ultrasonic cleaning, after cleaning, dries up.

8. the surface modifying method of biomaterial according to claim 1, is characterized in that: the described bioactive molecule fixedly successively with difference in functionality is ,-N ₃group position is fixing containing amino bioactive molecule, the fixing bioactive molecule containing sulfydryl (SH) in C=C position ,-NH ₂carboxylic bioactive molecule is fixed in position,-NHS group position is fixing containing amino bioactive molecule, containing amino bioactive molecule, be hirudin, protein (albumin, antithrombase, Fibrinogen etc.) or polypeptide (arginine-glycine-aspartic acid (RGD), arginine-glutamic acid-aspartic acid-valine (REDV) etc.); Bioactive molecule containing sulfydryl (SH) is mercapto-polyglycol (SH-PEG); Carboxylic bioactive molecule is extracellular matrix protein (fibronectin, laminin，LN, bone morphogenetic protein etc.), cell growth factor (endothelial cell growth factor (ECGF) (VEGF) etc.), albumin or heparin.

9. the surface modifying method of biomaterial according to claim 8, it is characterized in that: the fixing bioactive molecule containing sulfydryl of material surface position of double bond is that polyfunctional group surface modifying material is immersed into bioactive molecule (2～40mM) and DMPA(2～20mM) mixed solution in, 365nm irradiation under ultraviolet ray 3～5min, fully cleans with distilled water after taking-up; Material surface-NH ₂the fixing carboxylic bioactive molecule in position is that polyfunctional group surface modifying material is immersed into EDC(5～40mM)/(NHS, in the mixed solution of 5～20mM)/bioactive molecule (2～10mg/ml), fully react 2～8 hours, after taking-up, with distilled water, fully clean; Material surface-N ₃position is fixing is that polyfunctional group surface modifying material is immersed in the bioactive molecule solution of 1～10mg/ml containing amino bioactive molecule, and 365nm ultra-vioket radiation 3～5 minutes, fully cleans with distilled water after taking-up; Material surface-NHS group position is fixing is that surface modifying material is immersed in bioactive molecule (1～10mg/ml) solution containing amino bioactive molecule, and stirring at normal temperature reaction 2～8 hours is fully cleaned rear dry with distilled water.