CN104010672A - Blood separating device - Google Patents
Blood separating device Download PDFInfo
- Publication number
- CN104010672A CN104010672A CN201280063492.8A CN201280063492A CN104010672A CN 104010672 A CN104010672 A CN 104010672A CN 201280063492 A CN201280063492 A CN 201280063492A CN 104010672 A CN104010672 A CN 104010672A
- Authority
- CN
- China
- Prior art keywords
- assembly
- blood
- space
- lid
- separating mechanism
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000004369 blood Anatomy 0.000 title claims abstract description 112
- 239000008280 blood Substances 0.000 title claims abstract description 112
- 239000012530 fluid Substances 0.000 claims abstract description 8
- 238000000926 separation method Methods 0.000 claims description 15
- 239000000463 material Substances 0.000 claims description 13
- 238000007789 sealing Methods 0.000 claims description 13
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 11
- 239000000284 extract Substances 0.000 claims description 11
- 239000000741 silica gel Substances 0.000 claims description 11
- 229910002027 silica gel Inorganic materials 0.000 claims description 11
- 230000003373 anti-fouling effect Effects 0.000 claims description 6
- 239000011148 porous material Substances 0.000 claims description 5
- 239000012780 transparent material Substances 0.000 claims description 3
- 210000004623 platelet-rich plasma Anatomy 0.000 description 15
- 210000001772 blood platelet Anatomy 0.000 description 12
- 210000002381 plasma Anatomy 0.000 description 11
- 238000000034 method Methods 0.000 description 6
- 241000237970 Conus <genus> Species 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 210000003677 hemocyte Anatomy 0.000 description 4
- 229940000351 hemocyte Drugs 0.000 description 4
- 230000017531 blood circulation Effects 0.000 description 3
- 239000000470 constituent Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 230000002093 peripheral effect Effects 0.000 description 3
- 101150064138 MAP1 gene Proteins 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000036560 skin regeneration Effects 0.000 description 2
- 230000017423 tissue regeneration Effects 0.000 description 2
- 201000004384 Alopecia Diseases 0.000 description 1
- 208000008035 Back Pain Diseases 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 206010048768 Dermatosis Diseases 0.000 description 1
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 1
- 208000008930 Low Back Pain Diseases 0.000 description 1
- 102000013275 Somatomedins Human genes 0.000 description 1
- 231100000360 alopecia Toxicity 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 238000010241 blood sampling Methods 0.000 description 1
- 230000036770 blood supply Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 208000017520 skin disease Diseases 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/56—Labware specially adapted for transferring fluids
- B01L3/563—Joints or fittings ; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors
- B01L3/5635—Joints or fittings ; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors connecting two containers face to face, e.g. comprising a filter
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B7/00—Elements of centrifuges
- B04B7/08—Rotary bowls
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/10—Apparatus for enzymology or microbiology rotatably mounted
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/24—Apparatus for enzymology or microbiology tube or bottle type
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/041—Connecting closures to device or container
- B01L2300/042—Caps; Plugs
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0681—Filter
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0848—Specific forms of parts of containers
- B01L2300/0851—Bottom walls
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/06—Valves, specific forms thereof
- B01L2400/0633—Valves, specific forms thereof with moving parts
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5021—Test tubes specially adapted for centrifugation purposes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
- G01N33/491—Blood by separating the blood components
Abstract
In one embodiment of the present invention, provided is a blood separating device comprising: a first kit which comprises a first space for receiving blood on the inside thereof, and wherein the first space is separated between top and bottom by means of a first opening part; a second kit which comprises a second space for receiving blood on the inside thereof, and wherein the second space is separated between top and bottom by means of a second opening part; a plate which is disposed between the first kit and the second kit, and is provided with a through-connection hole for establishing fluid through connection of the first space and the second space; a stopper having a first bar which is provided projecting upwards from the plate and opens and closes the first opening part, and a second bar which is provided projecting downwards from the plate and opens and closes the second opening part; a first cap which covers the upper end part of the first kit; and a second cap which covers the bottom end part of the second kit. The stopper allows blood to be separated a plurality of times by respectively opening and closing the first opening part and the second opening part.
Description
Technical field
The present invention relates to blood separating mechanism, relate more specifically to a kind of blood separating mechanism that extracts PRP by centrifugalize.
Background technology
Blood is made up of blood plasma, platelet and hemocyte, and wherein, platelet comprises tissue regeneration and repairs required composition.When blood is carried out to centrifugalize, according to its weight, be separated into blood plasma, platelet and hemocyte, the platelet-shaped according to said method separating becomes the abundant blood plasma of blood plasma, be platelet rich plasma (PRP:Platelet Rich Plasma) and the platelet that lacks blood plasma, i.e. platelet poor plasma (PPP:Platelet Poor Plasma).Because platelet contains various somatomedin, wound healing and skin regeneration are had to important effect.Report demonstration, PRP contains this platelet with high concentration, and thus, various growth factors promote the breeding of peripheral cells, have the effect that the compositions such as collagen protein are synthesized in promotion fully.Thus, recently use PRP in various fields such as the ailing dermatosis such as treatment, treatment, skin regeneration or the burn treating of alopecia such as lumbago.
There is the blood that the method by for separating of this PRP is extracted and carry out centrifugalize, to blood cell composition and platelet poor plasma (PPP, Platelet Poor Plasma) separate, and again it is carried out to centrifugalize, and only extract the method for PRP.But the separation method that utilizes existing blood separating mechanism, there are the following problems, and after blood sampling, moving to the process of centrifugalize container, move in the process of the separation container that comprises filter element, blood can be exposed in air.And, utilize existing blood separating mechanism, be difficult to platelet to be concentrated into high concentration, and, be difficult to accurately extract required PRP amount.
Existing patent documentation has Korea S's patent document No. 10-2011-0045980.
Summary of the invention
Technical task
The present invention researches and develops in order to solve above-mentioned prior art problem, and object of the present invention be not exposed to blood under the state of air for providing a kind of, can carry out the blood separating mechanism of two times centrifugal separation.And object of the present invention is for providing a kind of blood separating mechanism that concentrated PRP can easily separate more.And object of the present invention is for providing a kind of blood separating mechanism that is accurately extracted required amount by an assembly.
Technical scheme
A kind of blood separating mechanism, is characterized in that, comprising: the 1st assembly, and inner side comprises the 1st space of containment blood, described the 1st space separates up and down by the 1st peristome; The 2nd assembly, inner side comprises the 2nd space of containment blood, described the 2nd space separates up and down by the 2nd peristome; Dish, is disposed between described the 1st assembly and described the 2nd assembly, has the intercommunicating pore that makes described the 1st space and described the 2nd space carry out fluid connection; Stop, has: the 1st bar (bar), and from the upwards side-prominent configuration of described dish, to open and close described the 1st peristome, and the 2nd bar, from the downward side-prominent configuration of described dish, to open and close described the 2nd peristome; The 1st lid, hides the upper end of described the 1st assembly; And the 2nd lid, hide the bottom of described the 2nd assembly, wherein, described stop is by opening and closing respectively described the 1st peristome and described the 2nd peristome, and carries out repeatedly blood separation.
One embodiment of the invention, described the 1st space and described the 2nd space, before injecting blood, keep vacuum state.
One embodiment of the invention, described stop is rotatably combined by screw with described the 1st assembly and described the 2nd assembly, and moves up and down.
Described the 1st assembly or described the 2nd assembly have the peristome for injecting or extract blood.
One embodiment of the invention, described peristome has the antifouling filter element of using.
One embodiment of the invention, described the 1st lid is rotatably combined by screw with described the 1st assembly, and moves up and down.
One embodiment of the invention, described the 2nd lid is rotatably combined by screw with described the 2nd assembly, and moves up and down.
One embodiment of the invention, described the 1st lid or described the 2nd lid comprise the parts of silica gel or quality of rubber materials.
One embodiment of the invention, more than one in described the 1st assembly, described the 2nd assembly, described stop, described the 1st lid and described the 2nd lid are formed by transparent material.
One embodiment of the invention, described the 1st lid or described the 2nd lid mark have graduation mark.
One embodiment of the invention, described the 1st assembly or described the 2nd assembly are be separated into upside space and lower side space hourglass-shaped.
One embodiment of the invention, between described stop and described the 1st assembly, and dispose the sealing ring of silica gel or quality of rubber materials between described stop and described the 2nd assembly.
Beneficial effect
According to the present invention, can provide a kind of and blood be not exposed under the state of air, can carry out the blood separating mechanism of two times centrifugal separation.And, a kind of blood separating mechanism that concentrates PRP more and can easily separate can be provided.And, a kind of blood separating mechanism that is accurately extracted required amount by an assembly can be provided.
Effect of the present invention is not defined in described effect, should be appreciated that the institute reasoning out in the structure that comprises the invention of recording from detailed explanation of the present invention or claims produces effect.
Brief description of the drawings
Fig. 1 is the cutaway view of the blood separating mechanism of one embodiment of the invention;
Fig. 2 is the view sub-anatomy of the blood separating mechanism of Fig. 1;
Fig. 3 and Fig. 4 are the sectional view of the blood separating mechanism of Fig. 1;
Fig. 5 is the partial cross section figure of the operation of the stop of the blood separating mechanism of signal displayed map 1;
Fig. 6 is the phantom that shows the antifouling collocation form with filter element;
Fig. 7 and Fig. 8 are the accompanying drawing that shows the true form of the blood separating mechanism of one embodiment of the invention.
Detailed description of the invention
Below, with reference to accompanying drawing, the present invention will be described.But the present invention realizes with various form, therefore, be not defined in embodiment described herein.And in order to clearly state in the accompanying drawings the present invention, omit the part irrelevant with explanation, and, by whole description, similar part is marked to similar reference numeral.
In whole description, certain part is while " being connected " with another part, the situation that comprises " directly connecting ", also comprise wherein between across the also situation of " indirect connection " of other parts.And, when certain part " comprises " a certain element, record without the special property opposed, not discharge other element, also comprise another element.
Reference accompanying drawing below, is elaborated to embodiments of the invention.
Fig. 1 is the cutaway view of the blood separating mechanism of one embodiment of the invention, Fig. 2 is the view sub-anatomy of the blood separating mechanism of Fig. 1, Fig. 3 and Fig. 4 are the sectional view of the blood separating mechanism of Fig. 1, Fig. 5 is the partial cross section figure of the operation of the stop of the blood separating mechanism of signal displayed map 1, and Fig. 6 shows the phantom of the antifouling collocation form with filter element.
With reference to Fig. 1 and Fig. 2, blood separating mechanism 1 comprises the 1st lid the 10, the 1st assembly 20, stop 30, the 2nd assembly 40 and the 2nd lid 50.According to this element, be formed for altogether 3 chambeies (spatial portion) of blood separation, open up to each other respectively or close in this type of chamber.
With reference to Fig. 3 and Fig. 4, each element is described in detail.First, the 1st lid 10 hides the upper end of the 1st assembly 20.The 1st lid 10 comprises: lateral part 11, is contacted with the external diameter of the upside cylindrical portion 21 of the 1st assembly 20 described below; And medial part 12, secure bond is in this lateral part 11, and is contacted with the internal diameter of the upside cylindrical portion 21 of the 1st assembly 20.
The internal diameter of the lateral part 11 of the 1st lid 10 has spire 13, and this spire 13 is rotatably combined by screw with the spire 26 of the upside cylindrical portion 21 that is positioned at the 1st assembly 20 described below.With respect to the 1st assembly 20, can move up and down with respect to the 1st assembly 20 by rotating the 1st lid 10, the 1 lids 10.Thus, can regulate the amount of the blood of the inner space (the 1st space) that is contained in the 1st assembly 20.
Medial part 12 secure bond of the 1st lid 10 are in lateral part 11.For example, as shown in Figure 3, the protuberance that is positioned at the side inner peripheral surface of lateral part 11 is aimed at the groove that inserts the side outer peripheral face that is positioned at medial part 13, and medial part 12 and lateral part 11 are fixed combination.
Medial part 12 for example, for having flexible material, the parts of silica gel or quality of rubber materials.Insert entry needle by medial part 12, blood can be injected to the inside of blood separating mechanism 1, also can extract from blood separating mechanism 1 blood of separation.Because medial part 12 forms by having flexible material, thereby medial part 12 can be combined closely in the upside cylindrical portion 21 of the 1st assembly 20.Thus, between the 1st lid 10 and the 1st assembly 20, even without other seal member, also can seal to prevent fluid leaks.
The 1st assembly 20 is disposed at the downside of the 1st lid 10, the 1st space that its inner side comprises containment blood.The 1st assembly 20 comprises: upside cylindrical portion 21; Downside cylindrical portion 22, one is connected in this upside cylindrical portion 21; Inner side conus portion 23, between upside cylindrical portion 21 and downside cylindrical portion 22, outstanding formation to the inside; Cervical region 24, the upside of conus portion 23 inside being formed at.Cervical region 24 has the 1st peristome 24a, and by the 1st peristome 24a, the 1st space is separated into upside space 20a and lower side space 20b up and down.
The upper end of the lateral surface of upside cylindrical portion 21 has the upside spire 26 with spire 13 combinations of above-mentioned the 1st lid 10.The bottom of the lateral surface of downside cylindrical portion 22 has the downside spire 27 with upside spire 35 combinations of stop 30 described below.Screw combination between downside cylindrical portion 22 and stop 30 is described as follows.
On the position that contacts with downside cylindrical portion 22 in upside cylindrical portion 21 of conus portion 23, inner side, trend inner space (the 1st space), forms more to upside, gradually little coniform of diameter.The upper end of inner side conus portion 23 has the cervical region 24 of the tubulose that upwards side direction extends to form.When being separated into upper and lower sides by these cervical region 24, the 1 spaces, while carrying out centrifugal blood separation, the constituent of blood occurs freely to move up and down.And, when the centrifugalize of blood finishes, by closing this cervical region 24, make blood carry out space separation.
Have for the peristome 25 that injects or extract blood in the side surface part of inner side conus portion 23.These peristome 25 compact configuration have not shown lid.This lid is made up of silica gel or quality of rubber materials, thus the blood in syringe is injected in blood separating mechanism 1, maybe the blood that completes separation is extracted to outside by syringe.
In addition, peristome 25 has the antifouling filter element of using.With reference to Fig. 6, peristome 25 is closely provided with antifouling with filter element 251, and on filter element 251, secure bond has sealing ring 252.And on sealing ring 252, there is peristome lid 253.Thus, when vacuum state in blood separating mechanism 1 is destroyed, open peristome lid 253, and by filter element 251, in blood separating mechanism 1, can carry out under the state of air communication, by other peristome 46 described below, blood is injected in blood separating mechanism 1, maybe by extremely outside the blood draw that completes separation.Stop 30 is disposed between the 1st assembly 20 and the 2nd assembly 40.Stop 30 comprises: outside cylindrical portion 31; Coil 32, be positioned at the internal diameter central part of outside cylindrical portion 31; The 1st bar (bar) 33, side-prominent setting to upside upwards above coiling 32 central part; The 2nd bar 34, side-prominent setting to downside faces down from coiling 32 central part.
Have and the upside spire 35 of downside spire 27 combinations of above-mentioned the 1st assembly 20 at the upside of the medial surface of outside cylindrical portion 31.Thus, stop 30 and the 1st assembly 20 rotatably carry out combination by screw, with respect to stop 30, the 1st assembly 20 are moved up and down, and can open and close whereby the 1st peristome 24a of the 1st assembly 20.
The downside of the medial surface of outside cylindrical portion 31 has the downside spire 36 with upside spire 47 combinations of the 2nd assembly 40 hereinafter described.Thus, stop 30 and the 2nd assembly 40, by rotatably combination of screw, with respect to stop 30, move up and down the 2nd assembly 40, can open and close whereby the 2nd peristome 43a of the 2nd assembly 40.
With reference to Fig. 5, with respect to stop 30, make upwards side shifting of the 1st assembly 20, the 1st peristome 24a of Open from This Side the 1st assembly 20, and with respect to stop 30, make the downward side shifting of the 2nd assembly 40, confirm thus the state of the 2nd peristome 43a that opens the 2nd assembly 40.
Referring again to Fig. 3 and Fig. 4, between stop 30 and the 1st assembly 20, dispose sealing ring 60.Sealing circle 60 is the parts of silica gel or quality of rubber materials, and sealing prevents that fluid leaks is between stop 30 and the 1st assembly 20 whereby.
And, between stop 30 and the 2nd assembly 40, dispose sealing ring 70.This sealing ring 70 is the parts of silica gel or quality of rubber materials, and sealing prevents that fluid leaks is between stop 30 and the 2nd assembly 40 whereby.
Dish 32 is lamellar, and this dish 32 has the intercommunicating pore 32a of up/down perforation.While overlooking, intercommunicating pore 32a is spherical, fan-shaped or semilune, but is not defined in this shape.By intercommunicating pore 32a, the inner space (the 2nd space) of the inner space of above-mentioned the 1st assembly 20 (the 1st space) and the 2nd assembly 40 hereinafter described carries out fluid connection., in fact the lower side space 20b of the 1st assembly 20 act as one with the upside space 40a of the 2nd assembly 40 hereinafter described.
The 1st bar 33 and the 2nd bar 34 are the parts of the set elongated cylindrical of diameter, but are not defined in this shape.The end of the 1st bar 33 and the 2nd bar 34 is contacted with the 1st peristome 24a and the 2nd peristome 43a, prevents blood flow, and in order to play the function that prevents this blood flow, the end of the 1st bar 33 and the 2nd bar 34 is formed as semi-spherical shape.
The 2nd assembly 40 is disposed at the downside of stop 30, the 2nd space that its inner side comprises containment blood.The 2nd assembly 40 has: upside cylindrical portion 41; Upside connecting portion 42, one is connected in this upside cylindrical portion 41, and diameter trend downside diminishes gradually; The cervical region 43 of tubulose, one is connected in the bottom of this upside connecting portion 42; Downside connecting portion 44, one is connected in this cervical region 43, and diameter becomes large gradually to downside; Downside cylindrical portion 45, one is connected in downside connecting portion 44.Cervical region 43 has the 2nd peristome 43a, and by the 2nd peristome 43a, the 2nd space is separated into upside space 40a and lower side space 40b up and down., the 2nd assembly 40 is formed as being separated into the hourglass-shaped of upside space 40a and lower side space 40b.
The upper end of the lateral surface of upside cylindrical portion 41 has the upside spire 47 with spire 36 combinations of above-mentioned stop 30.The bottom of the lateral surface of downside cylindrical portion 45 has the downside spire 48 with spire 52 combinations of the 2nd lid 50 hereinafter described.The combination of being undertaken by screw between downside cylindrical portion 45 and the 2nd lid 50 is described as follows.
And when being separated into upper and lower sides by cervical region 43, the 2 spaces of the 2nd assembly 40, when centrifugal blood separates, the constituent of blood occurs freely to move up and down.And, when the centrifugalize of blood completes, by closing cervical region 43, make blood carry out spatiality and separate.
The side surface part of upside connecting portion 42 has the peristome 46 for injecting or extract blood.This peristome 46 is closely provided with not shown lid.This lid is made up of silica gel or quality of rubber materials, thus the blood in syringe is injected in blood separating mechanism 1, maybe the blood that completes separation is passed through to syringe, extracts to outside.
The 2nd lid 50 is positioned at the downside of the 2nd assembly 40, to hide the bottom of the 2nd assembly 40.The 2nd lid 50 comprises the lateral part 51 of the external diameter of the downside cylindrical portion 45 that is contacted with above-mentioned the 2nd assembly 40.
The internal diameter of the lateral part 51 of the 2nd lid 50 has spire 52, and this spire 52 is rotatably combined by screw with the spire 48 of the downside cylindrical portion 45 that is positioned at above-mentioned the 2nd assembly 40.Rotate the 2nd lid 50 with respect to the 2nd assembly 40, thus, the 2nd lid 50 can move up and down with respect to the 2nd assembly 40.Can regulate whereby the blood flow volume of the inner space portion that is contained in the 2nd assembly 40.For example, in Fig. 3, the 2nd lid 50 is the state with respect to the 2nd assembly 40 downward side shiftings, and thus, it is large that the volume of lower side space 40b becomes, and in Fig. 4, the 2nd lid 50 is with respect to the upwards state of side shifting of the 2nd assembly 40, thus, and the smaller volume of lower side space 40b.
Between the 2nd lid 50 and the 2nd assembly 40, dispose sealing ring 80.Sealing circle 80 is the parts of silica gel or quality of rubber materials, and whereby, sealing is avoided between fluid leaks to the 2 lids 50 and the 2nd assembly 40.
Thus, blood separating mechanism 1 is divided into 3 spatial portions, i.e. the i.e. lower side space 20b of the 1st assembly 20 and the upside space 40a of the 2nd assembly 40 of the upside space 20a of the 1st assembly 20, the 2nd lower side space 40b of assembly 40 and the spatial portion of one.At this, the upside space 20a of the 1st assembly 20 separates by the 1st peristome 24a with lower side space 20b, and the upside space 40a of the 2nd assembly 40 separates by the 2nd peristome 43a with lower side space 40b.And stop 30, by opening and closing respectively the 1st peristome 24a and the 2nd peristome 43a, carries out repeatedly blood separation.This is explained as follows.
And the 2nd space of the 1st space of the 1st assembly 20 inside and the 2nd assembly 40 inside, before injecting blood, keeps vacuum state.The 1st space and the 2nd space remain vacuum state, thus, blood supply during to blood separating mechanism 1, are prevented from being difficult to situation about injecting because of internal air pressure.And in the time that blood injects, without manufacturing extraneous air circulation canal, therefore structure becomes simply, makes the injection of blood become easy.
And after manufacture, through after a while time, the vacuum in blood separating mechanism 1 is slowly destroyed.Thus, in the time of the vacuum maintaining in blood separating mechanism 1, opening under the state of some peristomes 25, also can inject blood by another one peristome 46.
And, each element of blood separating mechanism 1, in the 1st lid the 10, the 1st assembly 20, stop 30, the 2nd assembly 40 and the 2nd lid 50 is above is formed by transparent material.Thus, user can easily be observed amount or the released state of blood.
In addition, the 1st lid 10 or the 2nd lid 50 subscripts are marked with graduation mark, thus can Measurement accuracy or adjust the volume of the upside space 20a of the 1st assembly 20 and the lower side space 40b of the 2nd assembly 40.
In addition, in above-described embodiment, all have for the peristome 25,46 injecting or extract blood and be illustrated with the 1st assembly 20 and the 2nd assembly 40, also can only there is some in this peristome 25,46.And, also can be only on some in the 1st assembly 20 or the 2nd assembly 40, there are 2 above peristomes.
And, in above-described embodiment, be shaped as different being illustrated with the 1st assembly 20 and the 2nd assembly 40, but the shape of the 1st assembly 20 and the 2nd assembly 40 also can be identical, and its shape also can be relative.For example, the 1st assembly 20 also can be formed as hourglass-shaped.
And in above-described embodiment, with different being illustrated of shape of the 1st lid 10 and the 2nd lid 50, but the shape of the 1st lid 10 and the 2nd lid 50 also can be identical, and its shape also can be relative.For example, the 2nd lid 50 also can have the parts of silica gel or quality of rubber materials.
And, in above-described embodiment, be respectively arranged with upside spire 35 and downside spire 36 is illustrated with the medial surface of outside cylindrical portion 31, but the upside spire 35 of outside cylindrical portion 32 and downside spire 36 is some, or two lateral surface that are all arranged at outside cylindrical portion 32.The position of the downside spire 27 of this situation the 1st assembly 20 and the upside spire 47 of the 2nd assembly 40, also must change ground can with the position of the upside spire 35 of outside cylindrical portion 31 and 36 combinations of downside spire.
Fig. 7 and Fig. 8 are the accompanying drawing of the true form of the blood separating mechanism 1 of one embodiment of the invention.With reference to Fig. 7, can confirm the state that carries out the centrifugalize of blood by blood separating mechanism 1, with reference to Fig. 8, can confirm blood to inject the state of blood separating mechanism 1.
, utilize the blood separating mechanism 1 of one embodiment of the invention below, the method for carrying out twice centrifugal blood separation is described.
First, the blood of extraction is injected in blood separating mechanism 1 by the peristome 25 of the 1st assembly 20 or the peristome 46 of the 2nd assembly 40.Secondly, the 1st peristome 24a of the 1st assembly 20 closes by the 1st bar 33 of stop 30, and the 2nd peristome 43a of the 2nd assembly 40, under the state of opening, carries out the 1st centrifugalize.While completing centrifugalize for the first time, the blood plasma in blood and hemocyte layer are positioned at the upside space 40a of the 2nd assembly 40, and hemocyte is positioned at lower side space 40b.Now, by rotating the 2nd lid 50, suitably regulate the volume of lower side space 40b, thus, blood constituent is correctly separated.Afterwards, by the 2nd bar 34 of stop 30, close the 2nd peristome 43a of the 2nd assembly 40.Then, upset blood separating mechanism 1, the 1st assembly 20, with respect to the 2nd assembly 40, after being positioned at downside, under the state of the 1st peristome 24a of opening the 1st assembly 20, carries out the 2nd centrifugalize.Whereby, platelet rich plasma (PRP) and platelet poor plasma (PPP) layering.The 1st lid 10 that is positioned at downside is rotated, after suitably regulating the volume of upside space 20a of the 1st assembly 20, with respect to the 1st assembly 20 rotary stopper devices 30, thus, closes the 1st peristome 24a of the 1st assembly 20.In this way, the medial part 12 by the 1st lid 10 utilize syringe to extract the PRP in the blood separating.
Thus, because blood can be not externally carry out the 2nd centrifugalize from being exposed in blood separating mechanism 10, thus, do not exist because the worry infecting occurs blood to air, can extract highly enriched PRP.
The explanation of the invention described above is for illustrating, and general technical staff of the technical field of the invention should be appreciated that in the situation that not changing the technology of the present invention thought or essential feature, can be out of shape by other concrete form.Therefore above-described embodiment in all respects, only for illustrating, and and non-limiting the present invention.For example, separate enforcement with each element of single form explanation, similarly, separately the element of explanation also can be implemented with the form of combination.
Scope of the present invention shows according to claim, is to be understood that: the various changes that the meaning of accessory rights claimed range and scope and equal concept are carried out or the form of distortion, be all contained in scope of the present invention.
Claims (12)
1. a blood separating mechanism, is characterized in that, comprising:
The 1st assembly, inner side comprises the 1st space of containment blood, described the 1st space separates up and down by the 1st peristome;
The 2nd assembly, inner side comprises the 2nd space of containment blood, described the 2nd space separates up and down by the 2nd peristome;
Dish, is disposed between described the 1st assembly and described the 2nd assembly, has the intercommunicating pore that makes described the 1st space and described the 2nd space carry out fluid connection;
Stop, has: the 1st bar (bar), and from the upwards side-prominent configuration of described dish, to open and close described the 1st peristome, and the 2nd bar, from the downward side-prominent configuration of described dish, to open and close described the 2nd peristome;
The 1st lid, hides the upper end of described the 1st assembly;
And the 2nd lid, hide the bottom of described the 2nd assembly,
Wherein, described stop is by opening and closing respectively described the 1st peristome and described the 2nd peristome, and carries out repeatedly blood separation.
2. blood separating mechanism according to claim 1, is characterized in that, described the 1st space and described the 2nd space, before injecting blood, keep vacuum state.
3. blood separating mechanism according to claim 1, is characterized in that, described stop is rotatably combined by screw with described the 1st assembly and described the 2nd assembly, and moves up and down.
4. blood separating mechanism according to claim 1, is characterized in that, described the 1st assembly or described the 2nd assembly have the peristome for injecting or extract blood.
5. blood separating mechanism according to claim 4, is characterized in that, described peristome has the antifouling filter element of using.
6. blood separating mechanism according to claim 1, is characterized in that, described the 1st lid is rotatably combined by screw with described the 1st assembly, and moves up and down.
7. blood separating mechanism according to claim 1, is characterized in that, described the 2nd lid is rotatably combined by screw with described the 2nd assembly, and moves up and down.
8. according to a certain described blood separating mechanism in claim 1 to 7, it is characterized in that, described the 1st lid or described the 2nd lid comprise the parts of silica gel or quality of rubber materials.
9. according to a certain described blood separating mechanism in claim 1 to 7, it is characterized in that, more than one in described the 1st assembly, described the 2nd assembly, described stop, described the 1st lid and described the 2nd lid formed by transparent material.
10. according to a certain described blood separating mechanism in claim 1 to 7, it is characterized in that, described the 1st lid or described the 2nd lid mark have graduation mark.
11. according to a certain described blood separating mechanism in claim 1 to 7, it is characterized in that, described the 1st assembly or described the 2nd assembly are be separated into upside space and lower side space hourglass-shaped.
12. according to a certain the described blood separating mechanism in claim 1 to 7, it is characterized in that, between described stop and described the 1st assembly, and between described stop and described the 2nd assembly, disposes the sealing ring of silica gel or quality of rubber materials.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR10-2011-0138589 | 2011-12-20 | ||
| KR1020110138589A KR101170028B1 (en) | 2011-12-20 | 2011-12-20 | Blood separating apparatus |
| PCT/KR2012/001235 WO2013094819A1 (en) | 2011-12-20 | 2012-02-20 | Blood separating device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104010672A true CN104010672A (en) | 2014-08-27 |
| CN104010672B CN104010672B (en) | 2016-01-13 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201280063492.8A Active CN104010672B (en) | 2011-12-20 | 2012-02-20 | Blood separating mechanism |
Country Status (3)
| Country | Link |
|---|---|
| KR (1) | KR101170028B1 (en) |
| CN (1) | CN104010672B (en) |
| WO (1) | WO2013094819A1 (en) |
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| CN106040444A (en) * | 2015-04-09 | 2016-10-26 | 韩基洙 | Blood separating vial |
| CN108025320A (en) * | 2015-07-17 | 2018-05-11 | 统计诊断与创新有限公司 | The method and apparatus that sample is separated and collected |
| CN108992979A (en) * | 2018-07-19 | 2018-12-14 | 中国科学院东北地理与农业生态研究所 | A kind of mariages mouth combination unit of reagent exchange stored filter |
| CN110026297A (en) * | 2018-01-12 | 2019-07-19 | 瑞崴制造股份有限公司 | Body fluid separator |
| CN111194241A (en) * | 2017-09-05 | 2020-05-22 | 拜克门寇尔特公司 | Collecting and preparing blood samples for immediate diagnosis |
| CN111254045A (en) * | 2018-11-30 | 2020-06-09 | 瑞崴制造股份有限公司 | Kit for separating and concentrating humoral cells containing stem cells |
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| KR101626373B1 (en) * | 2015-02-04 | 2016-06-01 | 주식회사 지에스엠코리아 | Blood separating device |
| CN106669875B (en) * | 2017-02-13 | 2022-07-01 | 深圳市邦沃科技有限公司 | Platelet-rich plasma centrifuge tube and extraction method thereof |
| KR102119835B1 (en) * | 2017-11-22 | 2020-06-05 | (주)시지바이오 | Centrifuge device for separating blood components |
| US20210291162A1 (en) * | 2018-04-25 | 2021-09-23 | Global Life Sciences Solutions Usa Llc | Storage Vial |
| KR101892197B1 (en) * | 2018-06-20 | 2018-08-28 | (주)웰포트 | Apparatus for extracting platelet rich plasma |
| KR102527628B1 (en) * | 2019-05-20 | 2023-05-03 | (의) 삼성의료재단 | Container for tissue adhesiveness |
| KR102339809B1 (en) * | 2021-03-30 | 2021-12-16 | (주)레보메드 | Bio kit for extracting bodily fluids containing stem cells |
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| CN111194241A (en) * | 2017-09-05 | 2020-05-22 | 拜克门寇尔特公司 | Collecting and preparing blood samples for immediate diagnosis |
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| CN108992979B (en) * | 2018-07-19 | 2020-09-15 | 中国科学院东北地理与农业生态研究所 | Reagent exchange storage filtering double-thread-opening combined device |
| CN111254045A (en) * | 2018-11-30 | 2020-06-09 | 瑞崴制造股份有限公司 | Kit for separating and concentrating humoral cells containing stem cells |
| CN111254045B (en) * | 2018-11-30 | 2023-07-11 | 瑞崴制造股份有限公司 | Body fluid cell separation and concentration kit containing stem cells |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104010672B (en) | 2016-01-13 |
| WO2013094819A1 (en) | 2013-06-27 |
| KR101170028B1 (en) | 2012-08-01 |
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Address after: Gyeonggi Do, South Korea Patentee after: Ruiwei Medical Equipment Co.,Ltd. Guo jiahuodiqu after: Han Guo Address before: Gyeonggi Do, South Korea Patentee before: REV-MED, Inc. Guo jiahuodiqu before: Han Guo |
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