CN103940752A - Method for detecting antigen-antibody in 96-microplate in backside manner - Google Patents
Method for detecting antigen-antibody in 96-microplate in backside manner Download PDFInfo
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- CN103940752A CN103940752A CN201410170850.6A CN201410170850A CN103940752A CN 103940752 A CN103940752 A CN 103940752A CN 201410170850 A CN201410170850 A CN 201410170850A CN 103940752 A CN103940752 A CN 103940752A
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- microplate
- microwell plate
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Abstract
The invention relates to a method for detecting antigen-antibody in 96-microplate in a backside manner. A biosensor is bonded at the bottom of a bottomless 96-microplate, to-be-detected biological samples with different concentrations are injected into wells of the microplate, light emitted from an incident light source is vertically irradiated to the surface of the biosensor at the bottom of the microplate through a coupling fiber, and reflected light vertically emerging out from the bottom of the microplate is coupled to a spectrum analyzer through optical fibers; the combination condition of the biological samples in the wells is analyzed by analyzing change of the resonance peak; the biological samples nearby the biosensor at the bottom of the well are detected at the bottom of the microplate without influence of unevenness of the biological samples in the wells; the spectrum analyzer is utilized to realize dynamic monitoring to the characteristics of the biological samples, therefore, the detecting real-time property is realized; the method is simple to operate, has high processing speed, reduces error caused by manual operation and greatly improves the direction efficiency.
Description
Technical field
The present invention relates to a kind of optical detecting method, particularly a kind of back-illuminated type detects the method for antigen-antibody in 96-microwell plate.
Background technology
Optical detecting method is being brought into play more and more important effect in Modern high-tech industry.Traditional manual detection method, not only wastes time and energy, and error is large, and efficiency is low.Comparatively speaking, optical detecting method is without the robotization that contacts and can realize determination and analysis.
Secondly, when light is detected by normal incidence, the variable concentrations of the biological sample in different wells can absorb the light of different amounts, and the biological sample of different amounts also can cause catoptrical difference, thereby causes testing result error ratio larger.
So, how to solve said problem above, become one of problem of the anxious wish solution of association area.
Summary of the invention
The present invention be directed to that present optical detection is wasted time and energy, error is large, inefficient problem, a kind of back-illuminated type proposing detects the method for antigen-antibody in 96-microwell plate, particularly detecting protein bound ability, the quantity of bound analyte, chemical affinity between receptor agents and analyte, and detect have bacteriostatic activity natural products, detect antibacterial substance vigor, detect in the application such as output of antibacterial substance in fermentation liquor and play an important role.Detection method is simple, easy to operate.
Technical scheme of the present invention is: a kind of back-illuminated type detects the method for antigen-antibody in 96-microwell plate, biology sensor is adhered to the bottom of bottomless 96-microwell plate, in microwell plate well, inject the biological sample to be measured of variable concentrations, the light that incident light light source sends is the biosensor surface to microwell plate bottom through optical fiber vertical irradiation, from the reflected light of microwell plate bottom vertical outgoing through coupling fiber to spectroanalysis instrument.By analyzing the variation of resonance peak, thereby analyze the combination situation of biological sample in well.
Described incident light all can be the parallel optical fiber of array with catoptrical coupled fiber.
Beneficial effect of the present invention is: back-illuminated type of the present invention detects the method for antigen-antibody in 96-microwell plate, by microwell plate floor detection, mainly detects near the biological sample of shaft bottom biology sensor, can not be subject to the impact of biological sample unevenness in well; Utilize spectroanalysis instrument can realize the dynamic monitoring of biological sample characteristic, realize the real-time detecting; Method is simple to operate, and processing speed is fast, has reduced the error that manual operations brings, and has improved significantly detection efficiency.
Brief description of the drawings
Fig. 1 is the side schematic view of the embodiment of the present invention.
Embodiment
The side schematic view of embodiment as shown in Figure 1, adheres to bottomless 96-microwell plate 2 backs by biology sensor 3, realizes the detection of biological sample.In microwell plate 2 wells, put into the biological sample to be measured 1 of variable concentrations, launch a branch of incident light by optical fiber 4, impinge perpendicularly on the back of microwell plate 2, collect from the reflected light of the vertical outgoing in back of microwell plate 2 by optical fiber 5, and sent into spectroanalysis instrument, by analyzing the variation of resonance peak, thereby analyze the difference of biological sample in well and change.By analyzing the variation of resonance peak, thereby analyze the combination situation of biological sample in well, can realize the dynamic monitoring of biological sample characteristic, realize real-time and detect.
Can realize by mobile microwell plate 2 or optical fiber the detection of different wells.In order to improve detection efficiency, the optical fiber of single can be changed into eight parallel optical fiber, in like manner, collect catoptrical optical fiber and also can be replaced by eight parallel optical fiber.So, detection efficiency can improve several times, and meanwhile, the effect detecting is in real time also better.
Utilize back-illuminated type to detect reaction and the characteristic in differential responses stage of biological sample in microwell plate, reduced by biological sample height difference in different wells, to the absorption of the light also different errors of bringing.By changing optical fiber 4 and optical fiber 5 into eight parallel optical fiber, will greatly improve so detection rates, can realize better real-time detection.
Back-illuminated type detection method provided by the invention, detects for a large amount of 96-microwell plate combining with biological sample at present.This method can realize high flux by a small amount of biological sample and detect, and has effectively saved biological sample resource.
The invention provides a kind of back-illuminated type detection method, particularly detecting protein bound ability, the quantity of bound analyte, chemical affinity between receptor agents and analyte, and detect have bacteriostatic activity natural products, detect antibacterial substance vigor, detect in the application such as output of antibacterial substance in fermentation liquor and play an important role.
The method also can be used for little molecular screening, cell proliferation, cytotoxicity, and activity screen, protein-protein interaction, and cell-membrane receptor expression etc.By this back-illuminated type detection method, the more accurately reaction of detection of biological sample and variation.
Claims (2)
1. the method for antigen-antibody in a back-illuminated type detection 96-microwell plate, it is characterized in that, biology sensor is adhered to the bottom of bottomless 96-microwell plate, in microwell plate well, inject the biological sample to be measured of variable concentrations, the light that incident light light source sends is the biosensor surface to microwell plate bottom through optical fiber vertical irradiation, from the reflected light of microwell plate bottom vertical outgoing through coupling fiber to spectroanalysis instrument, by analyzing the variation of resonance peak, thereby analyze the combination situation of biological sample in well.
2. back-illuminated type detects the method for antigen-antibody in 96-microwell plate according to claim 1, it is characterized in that, described incident light all can be the parallel optical fiber of array with catoptrical coupled fiber.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410170850.6A CN103940752A (en) | 2014-04-28 | 2014-04-28 | Method for detecting antigen-antibody in 96-microplate in backside manner |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410170850.6A CN103940752A (en) | 2014-04-28 | 2014-04-28 | Method for detecting antigen-antibody in 96-microplate in backside manner |
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| Publication Number | Publication Date |
|---|---|
| CN103940752A true CN103940752A (en) | 2014-07-23 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410170850.6A Pending CN103940752A (en) | 2014-04-28 | 2014-04-28 | Method for detecting antigen-antibody in 96-microplate in backside manner |
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| CN (1) | CN103940752A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030059855A1 (en) * | 2000-10-30 | 2003-03-27 | Sru Biosystems, Llc | Method and instrument for detecting biomolecular interactions |
| CN1879018A (en) * | 2003-09-22 | 2006-12-13 | Sru生物系统公司 | Label-free methods for performing assays using a colorimetric resonant optical biosensor |
| CN101057131A (en) * | 2004-06-28 | 2007-10-17 | Sru生物系统公司 | Integration of direct binding sensors with mass spectrometry for functional and structural characterization of molecules |
| CN102317781A (en) * | 2008-12-15 | 2012-01-11 | Sru生物系统公司 | Methods of Detection of Changes in Cells |
-
2014
- 2014-04-28 CN CN201410170850.6A patent/CN103940752A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030059855A1 (en) * | 2000-10-30 | 2003-03-27 | Sru Biosystems, Llc | Method and instrument for detecting biomolecular interactions |
| CN1879018A (en) * | 2003-09-22 | 2006-12-13 | Sru生物系统公司 | Label-free methods for performing assays using a colorimetric resonant optical biosensor |
| CN101057131A (en) * | 2004-06-28 | 2007-10-17 | Sru生物系统公司 | Integration of direct binding sensors with mass spectrometry for functional and structural characterization of molecules |
| CN102317781A (en) * | 2008-12-15 | 2012-01-11 | Sru生物系统公司 | Methods of Detection of Changes in Cells |
Non-Patent Citations (1)
| Title |
|---|
| 张大伟等: "导模共振光学生物传感器的研究", 《 中国光学学会2010年光学大会论文集》 * |
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Application publication date: 20140723 |