CN103932703B - The recording method of primate sleep electricity physiological signal and device - Google Patents
The recording method of primate sleep electricity physiological signal and device Download PDFInfo
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- CN103932703B CN103932703B CN201410195895.9A CN201410195895A CN103932703B CN 103932703 B CN103932703 B CN 103932703B CN 201410195895 A CN201410195895 A CN 201410195895A CN 103932703 B CN103932703 B CN 103932703B
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Abstract
The present invention relates to a kind of can system log (SYSLOG) primate sleep electricity physiological signal method. The present invention is made up of following steps: one, pick up simultaneously and record the four road electricity physiological signals of tested animal; Two, four road amplifier of creatures' physical electric signals devices adopt the structure of traditional instrumentation amplifier+main amplifier; Three, the digital output end of multichannel ADC is connected with flush bonding processor, and flush bonding processor is connected to TF card, and flush bonding processor is connected to small liquid crystal display; Four, the I2C HPI of flush bonding processor is connected to the I2C of real time clock circuit from machine interface; The output pin that wakes up of real time clock circuit is connected to the external interrupt pin 1 of flush bonding processor; The stand-by power supply pin of real time clock circuit is connected to the positive pole of silver oxide cell simultaneously; The output of five, magnetic induction MEMS is connected to the external interrupt pin 2 of flush bonding processor; Six, keyboard is directly connected on the universaling I/O port of flush bonding processor.
Description
Technical field
The present invention relates to a kind of can system log (SYSLOG) primate sleep electricity physiological signal method.
Background technology
Research to sleep, the many unsolved mysteries for untiing animals and humans brain have very important significance. EEG signals under record sleep state, and EEG signals and the electricity physiological signal such as the corresponding myoelectricity in moment, eye electricity are comprehensively analyzed, it is the important means of research humans and animals sleep state switching and Changing Pattern thereof. Owing to can not carry out abrasive experiment, test in living human's brain, the sleep electro physiology experiment carried out on the primate (such as macaque or baboon) being similar to the mankind is just particularly important.
Existing brain electricity, myoelectricity or eye electrographic device are generally of the features such as volume is big, power consumption is many, and animal can not on one's own initiative from coordinating carrying out of experiment. Therefore, adopt the electricity physiological signal of above-mentioned existing equipment record animal, be necessary for limiting wholly or in part the activity of animal, for instance macaque is placed in monkey chair fixing. Existing research shows, the sleep behavior causing baboon the change of " non-natural " is occurred by health limitations affect, for instance: the pattern of " sleep " to " regaining consciousness " state switching is disturbed, abnormal drowsiness and aprosexia etc. Although there is no similar research (restriction state impact on its sleep pattern) on macaque, but there are some researches show that the normal behaviour of macaque is had a significant effect by the restriction of monkey chair.
Embedded system all achieves breakthrough progress in power consumption and volume etc. in recent years. But reasonably select various embedded scheme, be recorded for primate sleep electricity physiological signal, be technical barrier urgently to be resolved hurrily.
Summary of the invention
The purpose of the present invention is intended to overcome the deficiencies in the prior art, it is provided that a kind of recording method being realized primate electricity physiological signal by embedded technology.
The recording method of primate of the present invention sleep electricity physiological signal is made up of following steps:
One, pick up simultaneously and record the four road electricity physiological signals of tested animal, every road signal amplifier has two Differential Input electrodes respectively: record end and reference edge, all No. four amplifiers export one jointly with reference to ground, its electromotive force is equal to the midpoint of supply voltage and ground, for the common-mode voltage by tested animal bodies, controlling can in the common-mode voltage range of normal operation at amplifier;Wherein, for amplifying/pick up the record end of the first via amplifier of EEG signals, it is directly placed on the cerebral cortex in frontal lobe portion of tested animal brain by surgical operation; Reference edge is then fixed on tested animal parietal bone central area; Record end and reference edge for amplifying/pick up No. second amplifier of electromyographic signal are fixed on the both sides of tested Primate biceps brachii m. respectively through surgical operation; Record end and reference edge for amplifying/pick up No. threeth amplifier of horizontal direction electro-ocular signal are fixed on tested Primate left eye socket of the eye left and right sides skull respectively through surgical operation; Record end and reference edge for amplifying/pick up No. fourth amplifier of vertical direction electro-ocular signal are fixed on the skull of both sides up and down of tested Primate left eye socket of the eye respectively through surgical operation; The reference ground that all No. four amplifiers export jointly is then fixed on brow ridge center, tested animal left and right;
Two, four road amplifier of creatures' physical electric signals devices adopt the structure of traditional instrumentation amplifier+main amplifier, wherein, the faint electrical biological signals of the Differential Input that electrode is inputted by instrumentation amplifier, be converted to the single-ended voltage signal relative to reference ground, be equal to supply voltage and the midpoint on ground relative to the electromotive force on reference ground; Main amplifier is made up of General operational amplifier, after being connected to instrumentation amplifier, is responsible for amplify more than 60dB relative to the single-ended signal on reference ground; The bioelectrical signals amplified relative to reference ground, process that No. four amplifiers export respectively, and it is connected to the analog input end of four-way ADC, be converted to digital signal;
Three, the digital output end of multichannel ADC is connected with the interface 1 of the SPI of flush bonding processor, and the SPI interface 2 of flush bonding processor is connected to TF card, and the SPI interface 3 of flush bonding processor is connected to small liquid crystal display;
Four, the I2C HPI of flush bonding processor is connected to the I2C of real time clock circuit from machine interface; The output pin that wakes up of real time clock circuit is connected to the external interrupt pin 1 of flush bonding processor; The stand-by power supply pin of real time clock circuit is connected to the positive pole of silver oxide cell simultaneously;
The output of five, magnetic induction MEMS is connected to the external interrupt pin 2 of flush bonding processor;
Six, keyboard is directly connected on the universaling I/O port of flush bonding processor.
The recording equipment of primate of the present invention sleep electricity physiological signal, is formed by with lower part:
One, four road amplifier of creatures' physical electric signals devices pick up simultaneously and record four road electricity physiological signals of tested Primate, every road amplifier of creatures' physical electric signals device has two Differential Input electrodes respectively, these two Differential Input electrodes respectively record end and reference edge, all four road amplifier of creatures' physical electric signals devices export one jointly with reference to ground, the electromotive force on this reference ground is equal to the 1/2 of the electromotive force of power supply and the electromotive force sum on ground, for the common-mode voltage by tested Primate health, controlling can in the common-mode voltage range of normal operation at amplifier of creatures' physical electric signals device; Wherein, for amplifying/pick up the record end of the first via amplifier of creatures' physical electric signals device of EEG signals, it is directly placed on the cerebral cortex in frontal lobe portion of tested Primate brain; Reference edge for amplifying/pick up the first via amplifier of creatures' physical electric signals device of EEG signals is then fixed on tested Primate parietal bone central area; Record end and reference edge for amplifying/pick up the second road amplifier of creatures' physical electric signals device of electromyographic signal is individually fixed in the both sides of tested Primate biceps brachii m.; Record end and reference edge for amplifying/pick up the 3rd road amplifier of creatures' physical electric signals device of horizontal direction electro-ocular signal is separately fixed at tested Primate left eye socket of the eye left and right sides skull;Record end and reference edge for amplifying/pick up the 4th road amplifier of creatures' physical electric signals device of vertical direction electro-ocular signal is separately fixed at the skull of both sides up and down of tested Primate left eye socket of the eye; The reference ground that all four road amplifier of creatures' physical electric signals devices export jointly is then fixed on the center place between the brow ridge of tested Primate left and right;
Two, each road amplifier of creatures' physical electric signals device in four road amplifier of creatures' physical electric signals devices is all made up of traditional instrumentation amplifier and main amplifier, wherein, the faint electrical biological signals that Differential Input electrode is inputted by instrumentation amplifier, be converted to the single-ended voltage signal relative to reference ground, be equal to the 1/2 of the electromotive force of power supply and the electromotive force sum on ground relative to the electromotive force on reference ground; Main amplifier is made up of General operational amplifier, after being connected to instrumentation amplifier, is responsible for amplify more than 60dB relative to the single-ended voltage signal on reference ground; The bioelectrical signals amplified relative to reference ground, process that four road amplifier of creatures' physical electric signals devices export respectively, the signal after amplification is connected to the analog input end of four-way ADC, and the signal after amplification is converted to digital signal;
Three, the digital output end of four-way ADC is connected with the interface 1 of the SPI of flush bonding processor, and the SPI interface 2 of flush bonding processor is connected to TF card, and the SPI interface 3 of flush bonding processor is connected to small liquid crystal display;
Four, the I2C HPI of flush bonding processor is connected to the I2C of real time clock circuit from machine interface; The output pin that wakes up of real time clock circuit is connected to the external interrupt pin 1 of flush bonding processor; The stand-by power supply pin of real time clock circuit is connected to the positive pole of silver oxide cell simultaneously;
The output of five, magnetic induction MEMS is connected to the external interrupt pin 2 of flush bonding processor;
Six, keyboard is directly connected on the universaling I/O port of flush bonding processor.
Above-mentioned signal amplifier, flush bonding processor, TF card, real time clock circuit, magnetic induction and keyboard are all retrieved from prior art.
The structure of instrumentation amplifier+main amplifier that described amplifier of creatures' physical electric signals device adopts may refer to AnalogDeviceCorporation, " LowCost; LowPowerInstrumentationAmplifierAD620DataSheet (Rev.D) " [DB/OL], www.analog.com, 2001. and AnalogDeviceCorporation, " JFETInputInstrumentationAmplifierwithRail-to-RailOutputi nMSOPPackage (Rev.O) " [DB/OL], www.analog.com, 2006.
Four tunnel low-power consumption amplifier of creatures' physical electric signals devices are respectively used to amplify/pick up the brain electricity of tested Primate, eye electricity (each road of horizontal and vertical) and electromyographic signal. Thereafter then by analog/digital converter (Analog-to-DigitalConverter, ADC), four tunnels are converted to, through the analogue signal amplified, the digital signal that can store. ADC selects the model with more than four difference analogue input channels, such as MCP3308.
Flush bonding processor can adopt low-power consumption reduced instruction set computer (RISC) flush bonding processor, what be characterized as being under same treatment speed is low in energy consumption, its SPI (SerialPeripheralInterface, Serial Peripheral Interface (SPI)) interface 1 is connected to the digital output end of multichannel ADC, to obtain the multichannel bioelectrical signals after digitized. The SPI interface 2 of flush bonding processor is connected to TF card, in order to deposit the various bioelectrical signals that amplifier picks up in mass storage, use TF card (Trans-flashCard) as signal storage medium, and use file system (FileSystem) software wherein, it is characterized in that capacity is big, volume is little, low in energy consumption, and storage mass data is more reliable.The SPI interface 3 of flush bonding processor is connected to small liquid crystal display, in order to realize human-computer interaction function.
The I2C HPI of flush bonding processor is connected to the I2C of real time clock circuit from machine interface, and with this configuration realizing the time and reading; The output pin that wakes up of real time clock circuit is connected to the external interrupt pin 1 of flush bonding processor, in order to wake processor up in the suitable time so that it is enter recording status. Adopt the real time clock circuit (RealTimeClock having by interrupting arousal function, such as IC L1208, DS1337 etc.), it is characterized in the low-down power consumption retention time, and flush bonding processor to be waken up in the time needing system log (SYSLOG). Processor can be made to enter resting state in the time that need not record, and operationally wake processor up. The stand-by power supply pin of real time clock circuit is connected to the positive pole of silver oxide cell simultaneously, to ensure that at any time system has correct temporal information.
The output of magnetic induction MEMS is connected to the external interrupt pin 2 of flush bonding processor, in order to realize the non-contact switch to monitor when required by Magnet. Adopt magnetic induction MEMS (Micro-Electro-MechanicalSystems, mems device, such as A3212 etc.), MEMS can under very little volume and consumption conditions the minor variations of perception external magnetic field. In order to realize the Untouched control to monitor.
By method of the invention, it is possible to when being not intended to primate freedom of action and not affecting primate ortho sleep, the electricity physiological signal signal such as brain electricity under its sleep state of continuous non-volatile recording, myoelectricity, eye electricity and electrocardio. The recording equipment volume that this method adopts is little, lightweight, it is possible to be fixed on the head of primate, convenient and easy.
Accompanying drawing explanation
Fig. 1 be the present invention theory diagram.
Fig. 2 is a specific embodiment block diagram of the present invention.
Detailed description of the invention
The present invention is described further to provide below specific embodiment, but it is not used in the restriction present invention.
Fig. 2 is the physical circuit theory diagram according to thinking of the present invention design. Its core have employed has ARM7TDMI-S kernel, by the risc processor LPC2132 of grace intelligence Pu semiconductor production. LPC2132 is 32 arm processors of a new generation that power consumption is extremely low, there is the ability processing complexity, it is possible to meet present invention design to including that embedded system proposes: the various real-time process of file system, Man Machine Interface and multi-channel data acquisition. It addition, LPC2132 sheet is also integrated with various peripheral hardwares required for the present invention, including the on-chip memory (RAM and FLASHROM) of the real-time clock for timing mode, SPI interface and abundance for controlling TF storage card and LCD.
LPC2132 also has and has power managed function: under " dormancy " state, LPC2132 enters the king oiscillator halted state that power consumption is very low, and now LPC2132 kernel is owing to losing clock, and only internal storage state remains unchanged, and operating current is reduced to below 10uA. Due to the operating current of real-time clock integrated in LPC2132 very little (generally at about 10uA), and this real-time clock can LPC2132 in a dormant state time work independently, so LPC2132 is capable of above-mentioned " timing working " pattern. On daytime, tested animal is in the waking state without record, and now LPC2132 enters " resting state " of very power saving, only has real-time clock and keep timely function in system;In evening, when reaching the cut-in time that timing mode is arranged, the real-time clock being constantly in duty by interrupting, will wake the LPC2132 in " resting state " up and make it into " duty " of record data. In Fig. 2 in the lower left corner " the record silver oxide cell for the retention time " then when system main power source (poly-lithium battery) does not connect, power for the real time clock circuit in LPC2132, to prevent resetting system time after every time start.
Additionally in software aspects, in order to facilitate device and the PC systems exchange data equipped with commercial operation system (Windows, Linux etc.), in recording equipment, embedded system employs " file system " the software kit ZLGFS increased income. This document system can in the system of only small code size, it is achieved with the compatibility of PC file system.
In Fig. 2, the MEMS A3212 in the lower right corner, is that the Micro Energy Lose magnetic field produced by Allegro company controls switching device. When A3212 will have artificial magnetic field to occur in outside, respond, by interrupt notification LPC2132, the control signal that under magnetic control pattern, experimentation personnel send.
In order to ensure the portability of system and sufficiently long working time, in device, the design of battery power supply system is very crucial. Poly-lithium battery is a kind of selection preferably, and it can under same volume and weight condition, it is provided that higher than more than the one times capacity given of common disposable alkaline cell. It addition, poly-lithium battery can repeated charge more than 100 times, and major part both passes through water-proofing treatment. To sum up the system shown in Fig. 2 employs poly-lithium battery that capacity is 4500mAH as main battery.
In order to according to specifically studying the parameters such as requirement, the mode of operation of flexible configuration recording equipment, current time, recording equipment leaves Man Machine Interface. In order to improve the portability of device, reducing the discomfort of tested animal wears, device have employed diagonal line length and is only the single color LCD MzL-05 of 1.4 inches. The operating current of MzL-05 only 1.5mA, while reducing device volume, effectively extends same volume and the working time of weight battery.
It addition, the recording equipment shown in Fig. 2 can record four road bioelectrical signals simultaneously, four groups of signal pickup circuit are respectively directed to brain electricity, myoelectricity, eye electricity and electrocardiosignal. AFE (analog front end) have employed analog amplify circuit AD623 and the MCP6002 of low-power consumption and constitutes signal pickup circuit, and analog to digital conversion circuit has then selected 13 ADCMCP3308 of four-way, fully differential.
Under duty, the primary processor LPC2132 in signal recording apparatus is operated in 10MHz dominant frequency, together with the IF card of the 2GB capacity of record data, and the small-sized data liquid crystal display MzL-05 of 1.4, its total power consumption is about 20mA3.3V; Under resting state, in recording equipment, all of equipment all enters the exquisite state that power consumption is extremely low, and its total power consumption is about 20uA3.3V. In the every day of the lower record sleep electricity physiological signal of 8 hours in working order, if the polymerization road lithium battery selecting capacity to be 4500mAH, recording equipment can after once charging, the continuous sleep electricity physiological signal recording tested animal for 22 days.
Picking up simultaneously and record the four road electricity physiological signals of tested animal, every road signal amplifier has two Differential Input electrodes respectively: record end and reference edge, and all No. four amplifiers export one jointly with reference to ground; Wherein, for amplifying/pick up the record end of the first via amplifier of EEG signals, it is directly placed on the cerebral cortex in frontal lobe portion of tested animal brain by surgical operation;Reference edge is then fixed on tested animal parietal bone central area and CZ (CentralZone); Record end and reference edge for amplifying/pick up No. second amplifier of electromyographic signal is individually fixed in the both sides of tested Primate biceps brachii m.; For amplifying/the record end of No. threeth amplifier of pickup level (X) direction electro-ocular signal and reference edge be separately fixed at the left and right sides of tested Primate left eye socket of the eye; Record end and reference edge for amplifying/pick up No. fourth amplifier of vertical (Y) direction electro-ocular signal is separately fixed at the both sides up and down of tested Primate left eye socket of the eye; The reference ground that all No. four amplifiers export jointly is then fixed on brow ridge center, tested animal left and right.
Finally, the package unit of battery is included, it is possible to loading in the Water-proof aluminium alloy shell that volume is 77 �� 52 �� 35mm (L �� W �� H), its gross weight can control at below 250g. This device can be fixed on the head of tested Primate.
Claims (1)
1. the recording equipment of a primate sleep electricity physiological signal, it is characterised in that formed by with lower part:
One, four road amplifier of creatures' physical electric signals devices pick up simultaneously and record four road electricity physiological signals of tested Primate, every road amplifier of creatures' physical electric signals device has two Differential Input electrodes respectively, these two Differential Input electrodes respectively record end and reference edge, all four road amplifier of creatures' physical electric signals devices export one jointly with reference to ground, the electromotive force on this reference ground is equal to the 1/2 of the electromotive force of power supply and the electromotive force sum on ground, for the common-mode voltage by tested Primate health, controlling can in the common-mode voltage range of normal operation at amplifier of creatures' physical electric signals device; Wherein, for amplifying/pick up the record end of the first via amplifier of creatures' physical electric signals device of EEG signals, it is directly placed on the cerebral cortex in frontal lobe portion of tested Primate brain; Reference edge for amplifying/pick up the first via amplifier of creatures' physical electric signals device of EEG signals is then fixed on tested Primate parietal bone central area; Record end and reference edge for amplifying/pick up the second road amplifier of creatures' physical electric signals device of electromyographic signal is individually fixed in the both sides of tested Primate biceps brachii m.; Record end and reference edge for amplifying/pick up the 3rd road amplifier of creatures' physical electric signals device of horizontal direction electro-ocular signal is separately fixed at tested Primate left eye socket of the eye left and right sides skull; Record end and reference edge for amplifying/pick up the 4th road amplifier of creatures' physical electric signals device of vertical direction electro-ocular signal is separately fixed at the skull of both sides up and down of tested Primate left eye socket of the eye; The reference ground that all four road amplifier of creatures' physical electric signals devices export jointly is then fixed on the center place between the brow ridge of tested Primate left and right;
Two, each road amplifier of creatures' physical electric signals device in four road amplifier of creatures' physical electric signals devices is all made up of traditional instrumentation amplifier and main amplifier, wherein, the faint electrical biological signals that Differential Input electrode is inputted by instrumentation amplifier, be converted to the single-ended voltage signal relative to reference ground, be equal to the 1/2 of the electromotive force of power supply and the electromotive force sum on ground relative to the electromotive force on reference ground; Main amplifier is made up of General operational amplifier, after being connected to instrumentation amplifier, is responsible for amplify more than 60dB relative to the single-ended voltage signal on reference ground; The bioelectrical signals amplified relative to reference ground, process that four road amplifier of creatures' physical electric signals devices export respectively, the signal after amplification is connected to the analog input end of four-way ADC, and the signal after amplification is converted to digital signal;
Three, the digital output end of four-way ADC is connected with the interface 1 of the SPI of flush bonding processor, and the SPI interface 2 of flush bonding processor is connected to TF card, and the SPI interface 3 of flush bonding processor is connected to small liquid crystal display;
Four, the I2C HPI of flush bonding processor is connected to the I2C of real time clock circuit from machine interface; The output pin that wakes up of real time clock circuit is connected to the external interrupt pin 1 of flush bonding processor; The stand-by power supply pin of real time clock circuit is connected to the positive pole of silver oxide cell simultaneously;
The output of five, magnetic induction MEMS is connected to the external interrupt pin 2 of flush bonding processor;
Six, keyboard is directly connected on the universaling I/O port of flush bonding processor.
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| US3850161A (en) * | 1973-04-09 | 1974-11-26 | S Liss | Method and apparatus for monitoring and counteracting excess brain electrical energy to prevent epileptic seizures and the like |
| CN102068251A (en) * | 2011-01-24 | 2011-05-25 | 北京航空航天大学 | Implantable front-end acquisition device for brain electrical signal and manufacturing method thereof |
| CN202397462U (en) * | 2011-12-20 | 2012-08-29 | 华南理工大学 | Intraoperative motor area function positioning system based on brain wave slow cortical potential (SCP) wavelet analysis |
| CN102894973A (en) * | 2012-09-25 | 2013-01-30 | 浙江大学 | ECoG (electrocorticogram) signal amplifier |
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| US9042974B2 (en) * | 2005-09-12 | 2015-05-26 | New York University | Apparatus and method for monitoring and treatment of brain disorders |
| US9162051B2 (en) * | 2006-09-21 | 2015-10-20 | Neuropace, Inc. | Treatment of language, behavior and social disorders |
| WO2014066855A1 (en) * | 2012-10-26 | 2014-05-01 | The Regents Of The University Of California | Methods of decoding speech from brain activity data and devices for practicing the same |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3850161A (en) * | 1973-04-09 | 1974-11-26 | S Liss | Method and apparatus for monitoring and counteracting excess brain electrical energy to prevent epileptic seizures and the like |
| CN102068251A (en) * | 2011-01-24 | 2011-05-25 | 北京航空航天大学 | Implantable front-end acquisition device for brain electrical signal and manufacturing method thereof |
| CN202397462U (en) * | 2011-12-20 | 2012-08-29 | 华南理工大学 | Intraoperative motor area function positioning system based on brain wave slow cortical potential (SCP) wavelet analysis |
| CN102894973A (en) * | 2012-09-25 | 2013-01-30 | 浙江大学 | ECoG (electrocorticogram) signal amplifier |
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