CN103900873A - Liquid flowing device of flow cytometer - Google Patents
Liquid flowing device of flow cytometer Download PDFInfo
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- CN103900873A CN103900873A CN201410103355.3A CN201410103355A CN103900873A CN 103900873 A CN103900873 A CN 103900873A CN 201410103355 A CN201410103355 A CN 201410103355A CN 103900873 A CN103900873 A CN 103900873A
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Abstract
The invention discloses a liquid flowing device of a flow cytometer. The liquid flowing device comprises a flow type pond, wherein the flow type pond is bonded with an outer wall ring of the flow type pond; the outer wall of the outer wall ring of the flow type pond is matched with the upper end of a clamping body of the flow type pond; a waste-liquid clamping block is fixed on the flow type pond by a fourth rubber washer; a supporting block is connected in a positioning groove hole of the clamping body of the flow type pond in a matching manner; the waste-liquid clamping block is fastened with the supporting block by a long screw and fastening screws; the top surface and the bottom surface of the supporting block are connected with the clamping body of the flow type pond and are fastened by fastening screws; the clamping body of the flow type pond is fixed in a positioning hole at the bottom end of the clamping body of the flow type pond by threaded connection between a screw cap and a needle pipe positioning component; a first pipe joint is screwed on the waste-liquid clamping block; and a second pipe joint and a third pipe joint are screwed at the two sides of the lower end of the flow type pond. The liquid flowing device disclosed by the invention has the advantages that the focusing process of sheath-liquid laminar flow, the focusing process of a sample to be detected under the action of sheath-liquid laminar flow and the process of forming a stable flow by a test sample in a continuous cavity body can be realized, so that the liquid-flow disturbance caused by the liquid flowing device based on separable type flow type pond is effectively avoided.
Description
Technical field
The present invention relates to a kind of liquid stream device that microsome in fluid sample is carried out to multiparameter determination and analysis, what more specifically relate to is a kind of liquid stream device of flow cytometer.
Background technology
At present, cell or particulate are done to determination and analysis and conventionally have two class methods: a class is static method, to testing sample, (containing cell or other particulate or the liquid of thin layer tissue) done after single or multiple lift processing, it is compressed on to (for example microslide) on thin slice statically, again its various performance characterization parameters are done to determination and analysis, the instrument of employing has ordinary optical microscope, fluorescent microscope or laser confocal microscope etc. conventionally, another kind of is dynamic method, allow cell or other particulate in testing sample flow through specific search coverage with constant speed " one by one ", utilize sniffer (for example photomultiplier, avalanche diode etc.) observation flows through the multiple performance characterization parameter of each cell or other particulate of this search coverage, and utilize computing machine to do data processing and classification on a large scale to the result of observation, the principle of work of Here it is flow cytometer, in " flow cytometry ultimate principle and practical technique " that the people such as " Practical Flow Cytometry " and Liang Zhihui who shows at Shapiro shows, introduce to some extent.
In flow cytometer, for cell or other particulate realized in testing sample flow through specific search coverage with constant speed " one by one ", the means that conventionally adopt are to utilize laminar flow " focusing " power of outside sheath fluid (for example physiological saline) to realize.Sheath fluid-testing sample " focusing " core apparatus of general flow cytometer as shown in Figure 1, sheath fluid forms gradually the laminar flow of " focusing " in the cavity C8 in former hypomere streaming pond 27, testing sample gradually reduces under laminar flow " focusing " power of sheath fluid, and forms constant flow rate in the cavity C7 in former epimere streaming pond 26.Liquid stream " focusing " device in this separate type streaming pond needs positioning accuracy request, the technological requirement of mirror finish and the seal request of laminating of former epimere streaming pond 26 and former hypomere streaming pond 27 center pits all very high.Can not realize minute surface without leaking under the condition of laminating, also must between former epimere streaming pond 26 and former hypomere streaming pond 27, add packing washer, this can affect the sheath fluid that forms stably stratified flow in the cavity C8 exit in former hypomere streaming pond 27 undoubtedly, and then can affect testing sample " focusing " stream; When the location of former epimere streaming pond 26 and former hypomere streaming pond 27 center pits is undesirable, can affect testing sample " focusing " position in the cavity C7 in former epimere streaming pond 26, depart from the ideal position of search coverage, and then affect the signal to noise ratio (S/N ratio) of detectable signal, in addition, this situation also can the former hypomere streaming of local interference pond 27 cavity C8 exit form the sheath fluid of stably stratified flow, and then can affect testing sample " focusings " and flow.Therefore, people need to have a kind of liquid stream device that adopts monoblock type streaming pond, are used for realizing " focusing " that testing sample forms constant flow rate under laminar flow " focusings " power of sheath fluid and flow.
Summary of the invention
The object of the invention is to overcome the above problem that prior art exists, a kind of liquid stream device of flow cytometer is provided, fluid sample is carried out to liquid stream fast and focus on.
For realizing above-mentioned technical purpose, reach above-mentioned technique effect, the present invention is achieved through the following technical solutions:
A kind of liquid stream device of flow cytometer, comprise streaming pond, described streaming pond and the outer wall ring structure glue adhesion of streaming pond, the outer wall of described streaming pond outer wall ring coordinates with hole wall microclearance, clamp upper end, streaming pond, in the clamp of described streaming pond, be placed with the second rubber washer, in the left and right side threaded hole of described streaming pond clamp, be screwed into symmetrically the first holding screw and the second holding screw, described the first holding screw and described the second holding screw are fastened on the outer wall of described streaming pond outer wall ring, on upper surface, described streaming pond, vertically fix waste liquid fixture block by the 4th rubber washer, in the location notch hole of clamp side, described streaming pond, be connected with back-up block, described waste liquid fixture block and described back-up block pass through long spiro nail, the first trip bolt and the second trip bolt are fastening, two sides up and down of described back-up block and the locating slot of described streaming pond clamp are connected, and with the 3rd trip bolt, the 4th trip bolt, the 5th trip bolt, the 6th trip bolt is fastening, described streaming pond clamp is threaded connection in the pilot hole of bottom that nut needle tubing positioning component is fixed on described streaming pond clamp, on described waste liquid fixture block, be screwed with the first pipe adapter, the both sides, lower end in described streaming pond are screwed with the second pipe adapter and the 3rd pipe adapter.
Described needle tubing positioning component comprises needle tubing, described needle tubing and the adhesion of needle tubing guide pillar, and described needle tubing guide pillar is threaded with fastening pressure head, and described needle tubing and the second sample tubule joint closely cooperate, and described the second sample tubule joint is connected with sample tubule.
The invention has the beneficial effects as follows:
(1) process that flow cytometer liquid of the present invention stream device can be realized sheath fluid laminar flow " focusings " process, testing sample " focusings " process and test sample formation steady flow under sheath fluid Laminar Flow all, in a continuous cavity, has been avoided flowing based on the liquid in separate type streaming pond the liquid flow disturbance that device brings effectively.
(2) flow cytometer liquid stream device of the present invention, under positioning clamping device, can be realized precision positioning and the clamping in monoblock type streaming pond.
(3) the needle tubing positioning component of flow cytometer of the present invention can be realized location and the clamping of needle tubing.
(4) utilize needle tubing guide pillar and the streaming pond clamp in flow cytometer liquid stream device of the present invention, can realize the high precision right alignment in needle tubing and streaming pond.
(5) flow cytometer liquid of the present invention stream device can be realized testing sample and in streaming pond, forms while stablizing constant current, and the deviation of this constant current sample central shaft and streaming pond central shaft is in 5um.
Accompanying drawing explanation
Fig. 1 is sheath fluid-testing sample " focusing " device sketch of general flow cytometer of the present invention;
Fig. 2 is needle tubing positioning component wiring layout of the present invention;
Fig. 3 is lower the first sample tubule joint in needle tubing of the present invention location and the sectional view that coordinates of needle tubing;
Fig. 4 is the second sample tubule joint of the present invention and the sectional view that coordinates of needle tubing;
Fig. 5 is the wiring layout of applicable monoblock type streaming of the present invention pond precise positioning and clamping device;
Fig. 6 is flow cytometer liquid stream device lateral cross-sectional view of the present invention;
Fig. 7 is flow cytometer liquid stream device forward sectional view of the present invention;
Fig. 8 is flow cytometer liquid stream device rear view of the present invention;
Fig. 9 is the acquisition of signal schematic diagram based on flow cytometer liquid stream device of the present invention.
Number in the figure explanation: 1, the first pipe adapter, 2, long spiro nail, 3, waste liquid fixture block, 4, back-up block, 5, streaming pond clamp, 6, streaming pond, 7, streaming pond outer wall ring, 8, the second rubber washer, 9, needle tubing, 10, needle tubing guide pillar, 11, the second sample tubule joint, 11A, the first sample tubule, 12, sample tubule, 13, the 3rd rubber washer, 14, nut, 15, pressure head, 16, trip bolt, 17, adjust chuck, 18, square fixture block, 19, the first rubber washer, 20, precision pins, 21, the second holding screw, 22, the first holding screw, 23, the second pipe adapter, 24, the 3rd pipe adapter, 25, the 4th rubber washer, 26, the first trip bolt, 27, the second trip bolt, 28, the 3rd trip bolt, 29, the 4th trip bolt, 30, the 5th trip bolt, 31, the 6th trip bolt.
Embodiment
Below with reference to the accompanying drawings and in conjunction with the embodiments, describe the present invention in detail.
A kind of liquid stream device of flow cytometer, comprise streaming pond 6, structure glue adhesion is used with streaming pond outer wall ring 7 in described streaming pond 6, the outer wall of described streaming pond outer wall ring 7 coordinates with streaming pond clamp 5 hole wall microclearances, upper end, in described streaming pond clamp 5, be placed with the second rubber washer 8, in the left and right side threaded hole of described streaming pond clamp 5, be screwed into symmetrically the first holding screw 22 and the second holding screw 21, described the first holding screw 22 and described the second holding screw 21 are fastened on the outer wall of described streaming pond outer wall ring 7, on 6 upper surfaces, described streaming pond, vertically fix waste liquid fixture block 3 by the 4th rubber washer 25, in the location notch hole of clamp 5 sides, described streaming pond, be connected with back-up block 4, described waste liquid fixture block 3 passes through long spiro nail 2 with described back-up block 4, the first trip bolt 26 and the second trip bolt 27 are fastening, two sides up and down of described back-up block 4 and the locating slot of described streaming pond clamp 5 are connected, and with the 3rd trip bolt 28, the 4th trip bolt 29, the 5th trip bolt 30, the 6th trip bolt 31 is fastening, described streaming pond clamp 5 is threaded connection in the pilot hole of bottom that nut 14 needle tubing positioning components are fixed on described streaming pond clamp 5, on described waste liquid fixture block 3, be screwed with the first pipe adapter 1, the both sides, lower end in described streaming pond 6 are screwed with the second pipe adapter 23 and the 3rd pipe adapter 24.
Described needle tubing positioning component comprises needle tubing 9, described needle tubing 9 and 10 adhesions of needle tubing guide pillar, described needle tubing guide pillar 10 is threaded with fastening pressure head 15, and described needle tubing 9 and the second sample tubule joint 11 closely cooperate, and described the second sample tubule joint 11 is connected with sample tubule 12.
Installation step of the present invention is as follows:
Step 1) as shown in Figure 2,3, the first sample tubule joint 11A is compressed in needle tubing guide pillar 10 with being threaded of needle tubing guide pillar 10 through fastening pressure head 15, adopt again transition fit or gap fiting mode to insert needle tubing 9, and the bottom of needle tubing 9 is contacted with the top of the first sample tubule joint 11A, again by structure glue by needle tubing 9 and 10 adhesions of needle tubing guide pillar, back out again afterwards fastening pressure head 15 and take off the first sample tubule joint 11A, completing location and the assembling of needle tubing;
After structure glue is solidified, the second sample tubule joint 11 can be installed, and with fastening pressure head 15 compression that is threaded with needle tubing guide pillar 10, the state after compression is as shown in Figure 4.Wherein, the second sample tubule joint 11 closely cooperates on circular conical surface one annulus with needle tubing guide pillar 10, prevents the leakage problem of testing sample with this.The roundlet shell of column that the first sample tubule joint 11A and the difference of the second sample tubule joint 11 are the latter is than the former short 0 ~ 40um, prevent closely cooperating in process at the second sample tubule joint 11 and needle tubing guide pillar 10, second top of sample tubule joint 11 and the bottom of needle tubing 9 interfere
Step 2) first the second rubber washer 8 is placed in streaming pond clamp 5, prevent that with this sheath fluid from leaking; Be enclosed within on the multiaspect post in streaming pond 6 adjusting chuck 17, wherein, the cooperation of these two parts is that microclearance coordinates, and streaming pond 6 can be rotated with the rotation of adjusting chuck 17; Lower end cylinder wall and streaming pond clamp 5 precision-fit of chuck 17 will be adjusted again, realize its location on surface level with this, wherein, for streaming pond can be rotated in streaming pond clamp 5 with adjusting chuck 17, between the outer wall of streaming pond outer wall ring 7 and streaming pond clamp 5 upper end hole walls for microclearance coordinates, can also be at the outside surface figure of streaming pond outer wall ring 7 skim solid lubricant; Chuck 17 is adjusted in rotation again, precision pins 20 can be inserted in and adjust between the locating slot of chuck 17 and the pilot hole of clamp 5 upper surfaces, streaming pond, realizes its location in vertical sense of rotation with this; Place successively again the first rubber washer 19 and square fixture block 18, the trip bolt 16 of screwing on, streaming pond 6 is fastening downwards; Afterwards, the first holding screw 22 and the second holding screw 21 are tightened symmetrically in the side threaded hole of streaming pond clamp 5, be fastened on the outer wall of streaming pond outer wall ring 7; Unload successively afterwards precision pins 20, trip bolt 16, square fixture block 18, the first rubber washer 19 and adjust chuck 17;
Step 3) is placed on the 4th rubber washer 25 in the lower surface circular groove of waste liquid fixture block 3, and waste liquid fixture block 3 is pressed on the upper surface in streaming pond 6; The positioning cylinder of back-up block 4 sides is coordinated with streaming pond clamp 5 side location notch holes, another two sides of back-up block 4 coordinate with the locating slot of streaming pond clamp 5, and with the 3rd trip bolt 28, the 4th trip bolt 29, the 5th trip bolt 30,31 4 screw fastenings of the 6th trip bolt; Be screwed into long spiro nail 2, the first trip bolt 26 and 27, three screw fit of the second trip bolt fastening simultaneously, make waste liquid fixture block 3 vertically be fastened on streaming pond upper surface; The 3rd rubber washer 13 is enclosed within on needle tubing guide pillar 10; Again the needle tubing positioning component in step 1 is inserted in streaming pond clamp 5 bottom pilot holes; Again with the fastening needle tubing positioning component that is threaded between nut 14 and streaming pond clamp 5; Finally be screwed into successively the first pipe adapter 1, the second pipe adapter 23, the 3rd pipe adapter 24.
The detailed process that liquid is mobile:
As shown in Figure 6,7, testing sample is inputted from sample tubule 12, enters in " focusing " the chamber C3 in streaming pond 6 through needle tubing 9; Sheath fluid first flows in the current stabilization chamber C4 in streaming pond 6 through the tube chamber C6 of the second pipe adapter 23 or the tube chamber C5 of the 3rd pipe adapter 24, forms stable laminar flow; Afterwards, sheath fluid in " focusing " the chamber C3 in streaming pond 6 " focusing ", and to be in its " focusing " laminar flow central shaft testing sample produce " focusing " power; Under " focusing " power of sheath fluid, testing sample is meeting " focusing " fast in " focusing " the chamber C3 in streaming pond 6; Afterwards, " focusing " sheath fluid and sample flow in the detection cavity C2 in streaming pond 6; Last sheath fluid and tested sample and flow out flow cytometer liquid through the tube chamber C1 of the first pipe adapter 1 and flow device.
Shown in Fig. 9, laser, from the sidewall B1 vertical incidence in streaming pond 6, excites the sample after " focusing " in the search coverage in streaming pond 6; Can in the sidewall B2 perpendicular to streaming pond 6 and sidewall B4 direction, survey fluorescence or the side scattered light of sample stimulated emission, can in the sidewall B3 direction perpendicular to streaming 6, survey forward scattering light.
Principle of the present invention:
The material of described streaming pond outer wall ring 7 is wimet, is to use structure glue adhesion after transition fit or employing minim gap coordinate with streaming pond outer wall fit system again, avoids damaged for the protection of streaming pond in fastening process.
The first sample tubule joint 11A is for needle tubing 9 in needle tubing guide pillar 10 central axis direction location, and the second sample tubule joint 11 is for clamping sample tubule 12; Wherein, the small column segment length 10 ~ 40um of small column Length Ratio the second sample tubule joint 11 of the first described sample tubule joint 11A.
The first rubber washer 19 is for preventing sheath fluid and having tested the leakage of the mixed liquor of sample; The second rubber washer 8 is for preventing the leakage of sheath fluid; The 3rd rubber washer 13 is for preventing the leakage of sheath fluid; The 4th rubber washer is for the protection of streaming pond.
The first pipe adapter 1 is for delivery of sheath fluid and the mixed liquor of testing sample; The second pipe adapter 23 is for delivery of sheath fluid; The 3rd pipe adapter 24 is for monitoring or conveying or stable sheath fluid.
Adjust chuck 17, the polyhedron precision-fit in its inner polygon hole and streaming pond, makes the streaming pond can be with its rotation and movement; Adjust the face of cylinder, lower end of chuck 17 and the upper end face precision-fit of streaming pond clamp 5, on surface level, realize the precision positioning of the position in streaming pond; The threaded engagement of the upper surface of described adjustment chuck 17 and trip bolt 16, in the vertical direction is realized the fastening of streaming pond.
Described precision pins 20, for adjusting between the locating slot of chuck 17 and the accurate pin-and-hole of streaming pond clamp, is realized the precision positioning in the orientation in streaming pond 6 in vertical sense of rotation.
Claims (5)
1. the liquid of flow cytometer stream device, it is characterized in that: comprise streaming pond (6), structure glue adhesion is used with streaming pond outer wall ring (7) in described streaming pond (6), the outer wall of described streaming pond outer wall ring (7) coordinates with hole wall microclearance, streaming pond clamp (5) upper end, in described streaming pond clamp (5), be placed with the second rubber washer (8), in the left and right side threaded hole of described streaming pond clamp (5), be screwed into symmetrically the first holding screw (22) and the second holding screw (21), described the first holding screw (22) and described the second holding screw (21) are fastened on the outer wall of described streaming pond outer wall ring (7), on upper surface, described streaming pond (6), vertically fix waste liquid fixture block (3) by the 4th rubber washer (25), in the location notch hole of described streaming pond clamp (5) side, be connected with back-up block (4), described waste liquid fixture block (3) passes through long spiro nail (2) with described back-up block (4), the first trip bolt (26) and the second trip bolt (27) are fastening, two sides up and down of described back-up block (4) and the locating slot of described streaming pond clamp (5) are connected, and with the 3rd trip bolt (28), the 4th trip bolt (29), the 5th trip bolt (30), the 6th trip bolt (31) is fastening, described streaming pond clamp (5) is threaded connection in the pilot hole of bottom that nut (14) needle tubing positioning component is fixed on described streaming pond clamp (5), on described waste liquid fixture block (3), be screwed with the first pipe adapter (1), the both sides, lower end in described streaming pond (6) are screwed with the second pipe adapter (23) and the 3rd pipe adapter (24).
2. flow cytometer liquid stream device according to claim 1, it is characterized in that: described needle tubing positioning component comprises needle tubing (9), described needle tubing (9) is used the bonding mode adhesion of structure glue after adopting transition fit mode or adopt clearance fit with needle tubing guide pillar (10) again, for delivery of testing sample, described needle tubing guide pillar (10) is threaded with fastening pressure head (15), described needle tubing (9) closely cooperates with the second sample tubule joint (11), described the second sample tubule joint (11) is connected with sample tubule (12), described needle tubing (9) material is stainless steel or other wimet, its linearity is between 10 ~ 30um, described needle tubing guide pillar (10) upper end hole and needle tubing closely cooperate, and lower stomidium coordinates without leaking with described sample tubule (12) joint, and the lower stomidium of outer cylinder and described streaming pond clamp (5) closely cooperates, for location and the clamping of needle tubing.
3. flow cytometer liquid stream device according to claim 1, it is characterized in that: described streaming pond (6) adopts monoblock type streaming pond, the a part of outer wall in described streaming pond (6) is the face of cylinder, for its precision positioning and fastening, the outer wall of another part is multiple smooth faces, excitation laser is injected from a smooth face, and fluorescence signal or scattered light signal penetrate from other several smooth faces.
4. flow cytometer liquid stream device according to claim 1, is characterized in that:
Described the first rubber washer (19) is for preventing sheath fluid and having tested the leakage of the mixed liquor of sample; Described the second rubber washer (8) is for preventing the leakage of sheath fluid; Described the 3rd rubber washer (13) is for preventing the leakage of sheath fluid; Described the 4th rubber washer (25) is for the protection of streaming pond.
5. flow cytometer liquid stream device according to claim 1, is characterized in that: described the first pipe adapter (1) is for delivery of sheath fluid and the mixed liquor of testing sample; Described the second pipe adapter (23) is for delivery of sheath fluid; Described the 3rd pipe adapter (24) is for monitoring or conveying or stable sheath fluid.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410103355.3A CN103900873B (en) | 2014-03-19 | 2014-03-19 | A kind of fluidic device of flow cytometer |
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| CN201410103355.3A CN103900873B (en) | 2014-03-19 | 2014-03-19 | A kind of fluidic device of flow cytometer |
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| CN103900873B CN103900873B (en) | 2016-04-20 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106226223A (en) * | 2016-08-24 | 2016-12-14 | 桂林优利特医疗电子有限公司 | Particle analyzer |
| CN106706479A (en) * | 2016-12-29 | 2017-05-24 | 桂林优利特医疗电子有限公司 | Particle sheath flow imaging device |
| JP2018071648A (en) * | 2016-10-28 | 2018-05-10 | 東京応化工業株式会社 | Joint member, capillary unit, and screening device |
| CN108474729A (en) * | 2015-12-30 | 2018-08-31 | 生命技术公司 | The system and method that stabilized fluid stream is provided |
| CN114112872A (en) * | 2021-11-22 | 2022-03-01 | 常州必达科生物科技有限公司 | Device and method for calibrating sample flow position in flow type pool |
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| CN114112872A (en) * | 2021-11-22 | 2022-03-01 | 常州必达科生物科技有限公司 | Device and method for calibrating sample flow position in flow type pool |
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| CN103900873B (en) | 2016-04-20 |
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Effective date of registration: 20151210 Address after: Kolding road high tech Zone of Suzhou City, Jiangsu Province, No. 88 215000 Applicant after: SUZHOU INSTITUTE OF BIOMEDICAL ENGINEERING AND TECHNOLOGY, CHINESE ACADEMY OF SCIENCES Address before: Kolding road high tech Zone of Suzhou City, Jiangsu Province, No. 88 215000 Applicant before: Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences |
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Effective date of registration: 20160324 Address after: Science and Technology City kolding road high tech Zone of Suzhou City, Jiangsu Province, No. 88 215163 Applicant after: SUZHOU INSTITUTE OF BIOMEDICAL ENGINEERING AND TECHNOLOGY, CHINESE ACADEMY OF SCIENCES Address before: Kolding road high tech Zone of Suzhou City, Jiangsu Province, No. 88 215000 Applicant before: SUZHOU INSTITUTE OF BIOMEDICAL ENGINEERING AND TECHNOLOGY, CHINESE ACADEMY OF SCIENCES |
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Application publication date: 20140702 Assignee: Zhongsheng (Suzhou) Medical Instrument Co., Ltd. Assignor: SUZHOU INSTITUTE OF BIOMEDICAL ENGINEERING AND TECHNOLOGY, CHINESE ACADEMY OF SCIENCES Contract record no.: 2017320010008 Denomination of invention: Liquid flowing device of flow cytometer Granted publication date: 20160420 License type: Exclusive License Record date: 20170307 |
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