CN103627633A - Polymerase chain reaction kit - Google Patents

Polymerase chain reaction kit Download PDF

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Publication number
CN103627633A
CN103627633A CN201310616754.5A CN201310616754A CN103627633A CN 103627633 A CN103627633 A CN 103627633A CN 201310616754 A CN201310616754 A CN 201310616754A CN 103627633 A CN103627633 A CN 103627633A
Authority
CN
China
Prior art keywords
bracket
reagent box
pcr reagent
chain reaction
polymerase chain
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201310616754.5A
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Chinese (zh)
Inventor
刘志华
聂尚海
申跃华
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Suzhou Dongsheng Xingye Scientific Instrument Co Ltd
Original Assignee
Suzhou Dongsheng Xingye Scientific Instrument Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Suzhou Dongsheng Xingye Scientific Instrument Co Ltd filed Critical Suzhou Dongsheng Xingye Scientific Instrument Co Ltd
Priority to CN201310616754.5A priority Critical patent/CN103627633A/en
Publication of CN103627633A publication Critical patent/CN103627633A/en
Pending legal-status Critical Current

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B01L2300/024—Storing results with means integrated into the container

Abstract

The invention provides a polymerase chain reaction kit which comprises a bracket, wherein a hole used for accommodating a reagent reaction substance is formed in the bracket, and an information storage chip is arranged on the bracket. The information storage chip is used for storing relevant information and experiment detection information of the polymerase chain reaction kit. The detection information corresponds to an experiment amplification product, the detection information and the experiment amplification product are stored and transferred together, and thus automatic data exchange, searching and reading-writing between the polymerase chain reaction kit and a gene amplifier matched with the polymerase chain reaction kit are realized. The polymerase chain reaction kit realizes a management mode of uniformly archiving a polymerase chain reaction kit amplification product, polymerase chain reaction kit information and detection information, and solves the problem that the polymerase chain reaction kit amplification product and the experiment detection information are disordered and difficult to check.

Description

A kind of pcr reagent box
Technical field
The present invention relates to polymerase chain reaction device, particularly relate to a kind of pcr reagent box.
Background technology
In medical science and biology field, the DNA detection test kit of using polymerase chain reaction technology is widely adopted.These test kits require user that many parameters are set, as temperature, time, cycle number, reference material type, reference material numerical value, Case definition etc., these parameters directly determine the success or failure of PCR experiment, are the key factors that affects DNA detection result and diagnostic reliability, accuracy.
Common way is that this process has increased user's labour intensity, has reduced working efficiency, also strengthened time cost and error probability by the manual typing parameter one by one of user.Because many samples are micro-, disposable consumption, so the consequence that the mistake of DNA detection result causes cannot be retrieved sometimes.
Some producers have pre-installed reagent and reactant on porous plate, to reduce user's operation easier and labour intensity, but because the thermo-contact performance of heating system and porous plate is uncertain, often cause experimental result deviation.
Some test kit producers provide barcode and the Quick Response Code of printing, and its information comprising is reagent relevant information normally, as: test kit type, numbering, the date of production etc.These codings are on the one hand for the production of, storekeeper, logistics, on the other hand also for the automatic information of reagent necessary instrument read, validity period management.These ways make process control have basic guarantee, have reduced the error probability in DNA detection process.
The reagent producer of some technological precedences at present also provides the parameter for analyzing such as reference material type, reference material numerical value, calibration parameter, Case definition in bar code or Quick Response Code, makes the level of automation of DNA detection system higher.
Ubiquitous problem is: information is read-only, unidirectional character, only comprise the test kit relevant information of dispatching from the factory; And detection information is only retained in supporting detecting instrument in computer; Conventionally test tube is that isolate or random placement, and reagent amplified production corresponding detection information in kind is very easily upset after experiment completes, and is difficult to correspondence and verification afterwards.
The important value of PCR-based experiment and sample retrospective, check requirement, the pcr amplification product after having tested, monitoring information have value for preservation within a certain period of time.Require detection information to follow pcr amplification product correspondence in kind, storage, shift.Though test kit in the market has the porous plate of pre-installed reagents, do not there is electronic information storage function.
Summary of the invention
Based on this, be necessary easily to be upset for reagent amplified production after PCR experiment and experiment detection information, be difficult to the problem of correspondence and verification, a kind of pcr reagent box is provided.
A kind of pcr reagent box, described pcr reagent box comprises bracket, on described bracket, offer several in order to hold the hole of reagent react material, described bracket is provided with information storage chip, is also provided with the connecting interface that can be connected with gene-amplificative instrament on described bracket.
In an embodiment, described information storage chip is integrated in described bracket therein, and described information storage chip is radio-frequency card or flash chip.
In an embodiment, described bracket is provided with and prevents that experiment volume increase thing is to the closing means of external diffusion therein, and described closing means comprises preservative film and oil, and described oil is wrapped on the inwall of described hole by preservative film.
In an embodiment, described bracket is provided with temperature sensor therein.
In an embodiment, described bracket is provided with heating module therein.
In an embodiment, described heating module is comprised of chip Heating element therein.
In an embodiment, described bracket is provided with refrigeration module therein.
Therein in an embodiment, pre-packaged in described hole have a reagent react material.
In an embodiment, on described bracket, be also provided with the guide portion of indication plug direction therein.
In an embodiment, described guide portion is arranged at the edge of described bracket therein.
Above-mentioned pcr reagent box includes bracket, offers to hold the hole of reagent react material on bracket, is also provided with information storage chip on bracket.Described information storage chip is used for relevant information and the experiment detection information of store aggregated enzyme chain reaction test kit.Described detection information is corresponding with experiment amplified production, and both store together, shifts together, realize with supporting gene-amplificative instrament between automaticdata exchange, retrieval, read and write.Amplified product of polymerase chain reaction realized by such pcr reagent box and pcr reagent box information, detection information are unified the way to manage of filing, solved amplified product of polymerase chain reaction and tested detection information and upset, and being difficult to the problem of verification.
Accompanying drawing explanation
Fig. 1 is the schematic top plan view of pcr reagent box;
Fig. 2 is the side sectional view of pcr reagent box;
Fig. 3 is the enlarged view of I part in Fig. 2.
Embodiment
Below in conjunction with accompanying drawing, preferred embodiment of the present invention is described in detail, thereby so that advantages and features of the invention can be easier to be it will be appreciated by those skilled in the art that, protection scope of the present invention is made to more explicit defining.
Please refer to Fig. 1～2, an embodiment of the invention provide a kind of pcr reagent box 100.This pcr reagent box 100 comprises bracket 110, offers some in order to hold the hole 111 of testing reactive material on bracket 110.This bracket 110 is provided with for storing the information storage chip 120 of relevant information.On this bracket 110, be also provided with the connecting interface 130 for being connected with outside support equipment gene-amplificative instrament.
Bracket 110 is as plummer and the reaction platform of reagent react material, offer several thereon in order to hold the hole being evenly distributed 111 of testing reactive material, replaced the scattered experiment reaction tube in conventional apparatus, the form of unified structure, is convenient to carry out the operations such as pre-treatment, application of sample, reaction, cleaning on associate device like this.No matter be that solid-state or liquid reagent react material all can be packaged in this hole 111.Liquid reagent react material adopts the mode of overlay film to be made conventionally; Solid-state reagent react material conventionally adopt freeze-drying or solidify, semicure technique is made.After completing, reagent react material by modes such as coatings, is packaged in hole 111 again.In use, user only need add sample and/or water and just can complete the configuration of experiment reagent.Pre-packaged reagent react material can be simplified user's operation, thereby reduces misoperation probability, improves conventional efficient and accuracy rate.
Please refer to Fig. 3, bracket 110 is provided with closing means 140, and this closing means 140 is mainly in order to prevent that experiment volume increase thing is to external diffusion.In the present embodiment, closing means 140 comprises preservative film and oil, and oil is wrapped on the inwall that is fixed on hole 111 by preservative film.During use, preservative film is poked, oil flows out in hole 111 and swims in experiment volume increase thing top from preservative film, thereby reach, prevents that experiment volume increase thing is to the object of external diffusion.In other embodiments, can also adopt other mode, for example can adopt and in hole 111, pour in advance oil formation oil reservoir into, equipment by needle tubing and so on imports to experiment reagent the mode of oil reservoir below or adopts and mount thrust-augmenting nozzle on 111 inwall of hole again, oil flow into the mode in hole 111 through thrust-augmenting nozzle, be not specifically limited herein, as long as can meet, prevent that experiment volume increase thing is to the demand of external diffusion.
In bracket 110, be provided with temperature sensor 150, heating module 160 and/or refrigeration module.In the present embodiment, heating module 160 is comprised of chip Heating element, and refrigeration module is comprised of semiconductor cooler.Pcr reagent box 100 is connected with peripheral equipment gene-amplificative instrament, with respect to traditional mode of being carried out heated for controlling temperature by gene-amplificative instrament, can eliminates the error of bracket 110 and outside temperature control system, guarantee the reliability and stability of experiment reagent.In other embodiments, heating module 160 can also adopt type of heating such as printing heating line, Electric radiant Heating Film, semiconductor cooler to realize, and is not specifically limited herein.Refrigeration module also can adopt other refrigeration modes to realize, and can complete refrigerating function, is as the criterion, and is not specifically limited herein.
In bracket 110, be also provided with information storage chip 120.When pcr reagent box 100 dispatches from the factory, in information storage chip 120 is recorded in the production information of this pcr reagent box 100, validity period information, reference material information, Case definition information etc., so that gene-amplificative instrament supporting when supporting gene-amplificative instrament is connected can read and automatically configure correlation parameter automatically, guarantee that polymerase chain reaction meets the condition for peace of wanting of producer's regulation, and can make user obtain the diagnostic result of standard.When carrying out polymerase chain reaction, this information storage chip 120 can also be used to storage from pcr process monitoring information and the detected result information of supporting gene-amplificative instrament, and now the information of record is one, corresponding, transferable with experiment volume increase thing.Utilize supporting read-write equipment can realize information backup, retrieval, file, be beneficial to follow-up experimental result be verified.In present embodiment, this information storage chip 120 is radio-frequency cards, can also use flash chip in other embodiments, be not specifically limited herein, as long as can meet external system can, by the information on wired or wireless mode reading information storage chip 120, detect information and detected result and can be deposited into the requirement in information storage chip 120 simultaneously.
Bracket 110 is provided with a connecting interface 130, and this connecting interface 130 couples together pcr reagent box 100 and peripheral equipment gene-amplificative instrament.When pcr reagent box 100 being inserted into while connecting on peripheral equipment gene-amplificative instrament, owing to being integrated with temperature sensor 150, heating module 160 and/or refrigeration module on pcr reagent box 100, so can realize stable precision temperature, control.
Bracket 110 is provided with the guide portion 170 in order to indication plug direction,, when this pcr reagent box 100 is connected with peripheral equipment gene-amplificative instrament, plays guide effect, thereby avoids bracket 110 by dislocation, rotation, upset.In present embodiment, an angle of bracket 110 is different from other angles, and this different angle is guide portion 170, can avoid in use bracket 110 by dislocation, rotation, upset.In other embodiments, can also adopt the mode showing with arrow on bracket 110 to arrange and form guide portion 170, be not specifically limited herein, as long as can meet the demand of indication plug direction.
This is integrated with information storage chip 120, temperature sensor 150, the pcr reagent box 100 of heating module 160 and/or refrigeration module is connected with peripheral equipment gene-amplificative instrament by connecting interface 130, the relevant information of dispatching from the factory that not only can store aggregated enzyme chain reaction test kit 110, monitoring information and monitoring result during all right store aggregated enzyme chain reaction, facilitate follow-up backup, retrieval and file, amplified product of polymerase chain reaction and pcr reagent box 100 information have been realized, detection information is unified archive management, solved amplified product of polymerase chain reaction and tested detection information and upset, and be difficult to the problem of verifying.
The above embodiment has only expressed several embodiment of the present invention, and it describes comparatively concrete and detailed, but can not therefore be interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.

Claims (10)

1. a pcr reagent box, it is characterized in that, described pcr reagent box comprises bracket, on described bracket, offer several in order to hold the hole of reagent react material, described bracket is provided with information storage chip, is also provided with the connecting interface that can be connected with gene-amplificative instrament on described bracket.
2. pcr reagent box according to claim 1, is characterized in that, described information storage chip is integrated in described bracket, and described information storage chip is radio-frequency card or flash chip.
3. pcr reagent box according to claim 1, it is characterized in that, described bracket is provided with and prevents that experiment volume increase thing is to the closing means of external diffusion, and described closing means comprises preservative film and oil, and described oil is wrapped on the inwall of described hole by preservative film.
4. pcr reagent box according to claim 1, is characterized in that, described bracket is provided with temperature sensor.
5. according to the pcr reagent box described in claim 1 or 4, it is characterized in that, described bracket is provided with heating module.
6. pcr reagent box according to claim 5, is characterized in that, described heating module is comprised of chip Heating element.
7. pcr reagent box according to claim 1, is characterized in that, described bracket is provided with refrigeration module.
8. pcr reagent box according to claim 1, is characterized in that, pre-packaged in described hole have a reagent react material.
9. pcr reagent box according to claim 1, is characterized in that, is also provided with the guide portion of indication plug direction on described bracket.
10. pcr reagent box according to claim 9, is characterized in that, described guide portion is arranged at the edge of described bracket.
CN201310616754.5A 2013-11-27 2013-11-27 Polymerase chain reaction kit Pending CN103627633A (en)

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Application Number Priority Date Filing Date Title
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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017214906A1 (en) * 2016-06-15 2017-12-21 彭鹏 Reagent kit having cooling function, temperature display function, and wireless management function
WO2017214905A1 (en) * 2016-06-15 2017-12-21 彭鹏 Reagent kit having cooling function, loading reminder function, and wireless management function
WO2017214836A1 (en) * 2016-06-14 2017-12-21 彭鹏 Reagent kit having wireless management function and loading reminder function
WO2017214825A1 (en) * 2016-06-13 2017-12-21 石庆学 Reagent kit having thermal insulation, timing, sample adding reminding and wireless management functions
WO2017214866A1 (en) * 2016-06-15 2017-12-21 彭鹏 Reagent kit having cooling function, alarm function, and wireless management function
WO2017214786A1 (en) * 2016-06-13 2017-12-21 石庆学 Reagent kit having cold insulation function and wireless management function
WO2017214826A1 (en) * 2016-06-13 2017-12-21 石庆学 Reagent kit having cold insulation, alarming, wireless management and sample adding reminding functions
WO2017214837A1 (en) * 2016-06-14 2017-12-21 彭鹏 Reagent kit having music playback function and wireless management function
WO2017214760A1 (en) * 2016-06-12 2017-12-21 彭鹏 Reagent kit with timer function and remote management
WO2017214824A1 (en) * 2016-06-13 2017-12-21 石庆学 Reagent kit having cold insulation, temperature display, alarming, and wireless management functions

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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017214760A1 (en) * 2016-06-12 2017-12-21 彭鹏 Reagent kit with timer function and remote management
WO2017214825A1 (en) * 2016-06-13 2017-12-21 石庆学 Reagent kit having thermal insulation, timing, sample adding reminding and wireless management functions
WO2017214786A1 (en) * 2016-06-13 2017-12-21 石庆学 Reagent kit having cold insulation function and wireless management function
WO2017214826A1 (en) * 2016-06-13 2017-12-21 石庆学 Reagent kit having cold insulation, alarming, wireless management and sample adding reminding functions
WO2017214824A1 (en) * 2016-06-13 2017-12-21 石庆学 Reagent kit having cold insulation, temperature display, alarming, and wireless management functions
WO2017214836A1 (en) * 2016-06-14 2017-12-21 彭鹏 Reagent kit having wireless management function and loading reminder function
WO2017214837A1 (en) * 2016-06-14 2017-12-21 彭鹏 Reagent kit having music playback function and wireless management function
WO2017214906A1 (en) * 2016-06-15 2017-12-21 彭鹏 Reagent kit having cooling function, temperature display function, and wireless management function
WO2017214905A1 (en) * 2016-06-15 2017-12-21 彭鹏 Reagent kit having cooling function, loading reminder function, and wireless management function
WO2017214866A1 (en) * 2016-06-15 2017-12-21 彭鹏 Reagent kit having cooling function, alarm function, and wireless management function

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Application publication date: 20140312