CN103518698B - The sweep-out method of green alga in a kind of laver culture process - Google Patents
The sweep-out method of green alga in a kind of laver culture process Download PDFInfo
- Publication number
- CN103518698B CN103518698B CN201310424955.5A CN201310424955A CN103518698B CN 103518698 B CN103518698 B CN 103518698B CN 201310424955 A CN201310424955 A CN 201310424955A CN 103518698 B CN103518698 B CN 103518698B
- Authority
- CN
- China
- Prior art keywords
- green alga
- algicide
- laver
- sodium percarbonate
- sweep
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The present invention relates to a kind of method that suppresses chlorella growth, be particularly related to the sweep-out method of the green alga in a kind of laver culture process, first be the preparation of algicide: solid SODIUM PERCARBONATE is dissolved in deionized water, is mixed with SODIUM PERCARBONATE treatment fluid, the pH value of this treatment fluid is 9-10; Then be chosen at flood tide first 2 hours, by the algicide preparing be directly sprayed in laver culture be attached on the green alga frond on lace curtaining, on whole raft frame and whole piece hawser, the sprinkling frequency of this algicide is 1-3 month/time. The present invention adopts SODIUM PERCARBONATE as algicide, has nontoxic, odorless, pollution-free, water-soluble strong, facilitates adapted, environmental sound and inanimate object cumulative bad. Described green alga sweep-out method is very simple, convenient, and use cost is low, without rinsing, is subject to effect of natural conditions less, can use on a large scale. Algicide sprayed after 3 days, and the clearance rate of green alga is 100%, and the growth of laver is not formed to impact.
Description
Technical field
The present invention relates to a kind of method that suppresses chlorella growth, particularly green in a kind of laver culture processThe sweep-out method of algae.
Background technology
The cultivation of laver has long history in China, is traditional and compared with tool in China Water product aquacultureOne of representational industry, industry size is only second to sea-tangle and occupies second in the middle of algae. China laver is supportedGrow the occupation rate of industry in laver market, the world and constantly rise, especially the later stage nineties presents aobvious especiallyThe growing trend of work, China in 2000 surmounts Japan and suddenly becomes the first in the world laver culture big country, 2009The laver culture Liang Zhan world of year China cultivation total amount 65%, has initially increased by 54% than cultivation in 1971.
Laver growth cycle is divided into grows seedlings, collects seedling, nets training (putting into sea area) and the four-stage of gathering. And it is purpleIn the net training stage of dish, green alga will badly influence the growth of laver.
Annual late April starts, the middle ten days upper to September by thallus of porphyra maturation (carpospore cyst and anthreidForm) plant vegetables and be placed in the container that fills clean sea water, be attached to clam shell or oyster after diffusing carposporeOn shell, enter filamentous and cultivate, grow seedlings the stage. The seawater of growing seedlings is generally after being exposed to the sun and precipitatingClean sea water, so only have the spore of laver can be attached on lace curtaining, green alga can affect hardly laver and grow seedlingsProcess; By ripe filamentous, conchospore collects on preprepared laver lace curtaining, and this stage isCollect seedling the stage, green alga does not affect the process of collecting seedling yet; In 9 months, the last ten-days period laver adopted after seedling and lace curtaining can have been putEnter sea area, now, the green alga spore existing in sea area just can be attached on lace curtaining, raft frame and hawser. 10The middle of the month green alga raised growth, because sea area exists a large amount of green alga spores, it can competitiveness anchor at lace curtainingUpper, through the growth of 1 month, now on lace curtaining, growth had the long green alga of 10cm, and biomass is huge, andLaver is only seedling state, and its length is only 500 μ m left and right. So, the raised growth of green alga, seriousHinder the normal growth of laver.
Traditional green alga sweep-out method is: cold net method, lace curtaining is transported back on the bank, and put 20 days left sides of freezer intoRight; Seasoning, takes back lace curtaining on the bank, is placed on spacious place and is exposed to the sun one day. But the side that these are traditionalMethod can only partly be removed the green alga on lace curtaining, and the green alga on raft frame and hawser also can not be eliminated. Even processThe lace curtaining of processing, puts into the green alga that also can regrow out behind sea area again, has a strong impact on the percentage of product of laver,The income of laver is caused to huge loss.
In prior art, also useful acid-treated method is removed the green alga growing in laver culture process, as NingboUniversity discloses pickling agent and the processing method of porphyra haitanensis cultivation green algae and the cell of catching an illness, and publication number is101822271A, when the shortcoming of the method is preparating acid treatment fluid, not only can form harm to health,But also can cause the secondary pollution of ocean. Human body contacts its steam or smog, can cause acute poisoning, goes outThe conjunctivitis of losing face, nose and oral mucosa have burn feeling, gingiva bleeding gingival hemorrhage, tracheitis etc. Hydrochloric acid has danger to environmentEvil, can pollute water body and soil, and the hydrochloric acid of low concentration also can kill Marine Planktonic animals and plants, destroysThe ecological balance, and hydrochloric acid belongs to hazardous chemical, has environmental risk in storage process. Although, this patentIn point out that acid treatment reaches 90% to the clearance rate of green alga, but can cause larger impact, laver to laverThallus diminishes, and turns to be yellow etc., affects the quality of laver. Equally, the method also can not prevent that lace curtaining from putting into seaGreen alga continued growth behind district.
In laver culture, conventionally also adopt containing the agricultural chemicals of plant growth inhibitor and remove green alga, agricultural chemicals isOne class bioactivator, is again a class chemically reactive substance, can residue in environment, to specific after distributionIn environment, biocenological composition and variation cause that certain impacts, or with some other materials or thing in environmentBody interacts, and diffusion profile in specific environment, causes bad to the benign development of the ecosystemConsequence.
So algae removal agent or the growth inhibitor of high security have the very high market demand.
Summary of the invention
The object of this invention is to provide the sweep-out method of the green alga in a kind of laver culture process, this method adoptsSODIUM PERCARBONATE is as algicide, and can remove effectively and quickly green alga does not affect again the normal growth of laver simultaneously,And also can not pollute environment, not need rinsing, simple, easy to operate.
Object of the present invention can be achieved through the following technical solutions:
A sweep-out method for green alga in laver culture process, its step comprises:
In flood tide first 2 hours, algicide is directly sprayed on to the green alga on lace curtaining that is attached in laver cultureOn frond, on whole raft frame and whole piece hawser, the sprinkling frequency of this algicide is 1-3 month/time; Preferably, the sprinkling frequency of this algicide is 2 months/times;
The compound method of described algicide is, solid SODIUM PERCARBONATE is dissolved in deionized water, is mixed withSodium carbonate treatment fluid, the pH value of this treatment fluid is 9-10;
The valid density of the SODIUM PERCARBONATE in described SODIUM PERCARBONATE treatment fluid is 40-150mmol/L. Preferably,The valid density of the SODIUM PERCARBONATE in described SODIUM PERCARBONATE treatment fluid is 40-100mmol/L.
Sprinkling amount on the green alga frond of described algicide on lace curtaining is 1-6mL/cm2, this algicide is wholeSprinkling amount on individual raft frame and whole piece hawser is 120-170mL/ rice. Preferably, described algicide is on lace curtainingGreen alga frond on sprinkling amount be 2-3mL/cm2, the spray of this algicide on whole raft frame and whole piece hawserThe amount of spilling is 150mL/ rice.
Described laver is Porphyra yezoensis.
Compared with prior art, beneficial effect of the present invention is:
1, the present invention adopts SODIUM PERCARBONATE as algicide, removes the green alga growing in laver culture process,That SODIUM PERCARBONATE has is nontoxic, odorless, the advantage such as pollution-free, and chemical property is more stable, is convenient to transport, water-solubleProperty is strong, facilitates adapted, environmental sound and inanimate object cumulative bad.
2, the green alga sweep-out method in laver culture process provided by the invention is very simple, convenient, usesCost is low, without rinsing, is subject to effect of natural conditions less, can use on a large scale. Algicide sprayed after 3 days,The clearance rate of green alga is 100%, and the growth of laver is not formed to impact.
Brief description of the drawings
Fig. 1 is the growth rate comparison diagram that sprays the flat Enteromorpha sample after SODIUM PERCARBONATE treatment fluid.
Fig. 2 is chlorophyll fluorescence parameters maximum amount that sprays the flat Enteromorpha sample after SODIUM PERCARBONATE treatment fluidYield comparison figure.
Fig. 3 sprays after SODIUM PERCARBONATE treatment fluid, the photosynthetic rate after standing 120min and the variation of P/RFigure.
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further described:
Embodiment 1
In the present embodiment, choose Porphyra yezoensis culture zone, Jiangsu Rudong county raft frame as experimental subjects, concrete stepsAs follows:
(1), the preparation of algicide: solid SODIUM PERCARBONATE is dissolved in deionized water, is mixed with percarbonic acidSodium treatment fluid, pH value is 9.5, the valid density of the SODIUM PERCARBONATE in this treatment fluid is 100mmol/L.
(2), in flood tide first 2 hours, convenient operation when Porphyra yezoensis raft frame all surfaces. By step (1)In algicide be sprayed directly on the green alga frond being attached on lace curtaining, sprinkling amount is 2-3mL/cm2,Algicide is directly sprayed on whole raft frame and whole piece hawser, and sprinkling amount is 150mL/ rice. The spray of this algicideSpilling frequency is 2 months/times. In Porphyra yezoensis breeding process, fisherman almost can plunge into the commercial sea and carry out laver every dayThe arrangement of lace curtaining and maintenance, so, in the time plunging into the commercial sea arrangement and safeguard, can select in batches to lace curtaining, raft frameCarry out spraying operation with hawser, be subject to effect of natural conditions less, simple to operate, can use on a large scale.
Algicide was sprayed at after green alga frond upper 3 day, and frond is all dead, and the death rate reaches 100%,Naturally, in tidal phenomena, under the physical action of dead green alga in marine stream, fracture enters sea area, reachesTo the object of removing green alga, and the growth of Porphyra yezoensis is not formed to impact.
Embodiment 2
Experiment material: fresh, healthy flat waterside is adopted back in sea area and obtained after laboratory cultures in Jiangsu Rudong countyTongue sample.
In order to detect the inhibition of algicide provided by the invention to chlorella growth, be detected object by EnteromorphaTest:
SODIUM PERCARBONATE dissolution of solid, in distilled water, is mixed with to SODIUM PERCARBONATE valid density and isThe treatment fluid of 40-50mmol/L, is placed in watering can for subsequent use. Because the Enteromorpha quantity ratio of cultivating in laboratoryLess, do not have the Enteromorpha quantity in actual field many, so in the treatment fluid of preparation, SODIUM PERCARBONATE valid densityFor 40-50mmol/L just can suppress the normal growth of Enteromorpha.
The flat Enteromorpha sample of selecting some complete health, sucks surface moisture, is divided into 6 groups, and every group approximatelyFor 2cm2, spray wherein 3 groups with the above-mentioned SODIUM PERCARBONATE treatment fluid preparing is even, be labeled as experimental group,Every group of sprinkling amount is 4-6mL, and all the other 3 groups are as a control group, with distilled water spray, respectively leave standstill 30min,60, after 120min with seawater flushing, measure chlorophyll fluorescence parameters and the photosynthetic oxygen evolution speed of frond, thenAgain flat Enteromorpha sample is put into blake bottle renewal cultivation, observe the frond state variation of every day.
Test result shows:
The flat Enteromorpha sample grown rate of 1, spraying through SODIUM PERCARBONATE treatment fluid obviously declines, processing 30,60, after 120min more blank group declined respectively 57.96%, 28.50%, 84.50%, concrete as Fig. 1 instituteShow, as can be seen from Figure, SODIUM PERCARBONATE treatment fluid sprays after flat Enteromorpha sample, leaves standstill 120min, can make itSubstantially stop growing.
2, chlorophyll fluorescence parameters maximum amount suboutput Fv/Fm successively decreases along with processing time lengthening is graduallyTrend, when SODIUM PERCARBONATE treatment fluid sprays and leaves standstill after 120min, although can not be lethal rapidly, Fv/FmThan 50% left and right that declined of the blank group under the same terms, photosynthetic activity reduces, specifically as shown in Figure 2.
3, experiment combination control group is processed through spraying, and leaves standstill respectively after 120min the light of experimental group frondClose and put more blank group of oxygen speed and decline to a great extent, only have 1/5 of blank group; The respiratory rate of experimental group frondObviously raise, the P/R(photosynthetic oxygen evolution speed/respiratory rate of experimental group) value approached 1, lower than green algaNormal P/R value (normal P/R value=4), specifically as shown in Figure 3, the photosynthesis of illustrative experiment group EnteromorphaBy severe inhibition. After one week, the death that bleaches of experimental group Enteromorpha root, shows that SODIUM PERCARBONATE is to green alga frondGrowth has remarkable inhibition.
The above is preferred embodiment of the present invention, but the present invention should not be confined to this embodiment instituteDisclosed content. Do not depart from the equivalence or the amendment that under spirit disclosed in this invention, complete so every, all fallEnter the scope of protection of the invention.
Claims (5)
1. a sweep-out method for the green alga in laver culture process, its step comprises:
In flood tide first 2 hours, algicide is directly sprayed on to the green alga on lace curtaining that is attached in laver cultureOn frond, on whole raft frame and whole piece hawser, the sprinkling frequency of this algicide is 1-3 month/time; Except algaeSprinkling amount on the green alga frond of agent on lace curtaining is 1-6mL/cm2, algicide is at whole raft frame and whole piece cableSprinkling amount on rope is 120-170mL/ rice;
The compound method of described algicide is, solid SODIUM PERCARBONATE is dissolved in deionized water, is mixed withSodium carbonate treatment fluid, the valid density of the SODIUM PERCARBONATE in this SODIUM PERCARBONATE treatment fluid is 40-150mmol/L,PH value is 9-10.
2. the sweep-out method of the green alga in laver culture process according to claim 1, its feature existsIn: the valid density of the SODIUM PERCARBONATE in described SODIUM PERCARBONATE treatment fluid is 40-100mmol/L.
3. the sweep-out method of the green alga in laver culture process according to claim 1, its feature existsIn: the sprinkling amount on the green alga frond of algicide on lace curtaining is 2-3mL/cm2, algicide is at whole raft frameAnd sprinkling amount on whole piece hawser is 150mL/ rice.
4. the sweep-out method of the green alga in laver culture process according to claim 1, its feature existsIn: the sprinkling frequency of described algicide is 2 months/times.
5. according to the sweep-out method of the green alga in the laver culture process described in any one in claim 1-4,It is characterized in that: described laver is Porphyra yezoensis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310424955.5A CN103518698B (en) | 2013-09-17 | 2013-09-17 | The sweep-out method of green alga in a kind of laver culture process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310424955.5A CN103518698B (en) | 2013-09-17 | 2013-09-17 | The sweep-out method of green alga in a kind of laver culture process |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103518698A CN103518698A (en) | 2014-01-22 |
| CN103518698B true CN103518698B (en) | 2016-05-18 |
Family
ID=49921339
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310424955.5A Active CN103518698B (en) | 2013-09-17 | 2013-09-17 | The sweep-out method of green alga in a kind of laver culture process |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103518698B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110235773A (en) * | 2019-07-26 | 2019-09-17 | 国家海洋环境监测中心 | Seaweed raft frame green alga preventing control method based on micro- traditional font library abatement of aestivating |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107262439A (en) * | 2017-06-28 | 2017-10-20 | 中国科学院海洋研究所 | A kind of method for removing miscellaneous algae Enteromorpha on cultivation lace curtaining |
| CN109496826A (en) * | 2018-11-13 | 2019-03-22 | 国家海洋环境监测中心 | Huanghai Sea green tide preventing control method based on overwintering micro- traditional font library abatement |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4847089A (en) * | 1986-07-16 | 1989-07-11 | David N. Kramer | Cleansing and distinfecting compositions, including bleaching agents, and sponges and other applicators incorporating the same |
| CN102550388A (en) * | 2012-01-06 | 2012-07-11 | 上海海洋大学 | Method for removing green algae in the middle and late culture stages of Porphyra yezoensis |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0789595A4 (en) * | 1994-11-04 | 2000-11-02 | Betzdearborn Inc | Synergistic biocidal combinations |
| WO2007089235A1 (en) * | 2006-02-03 | 2007-08-09 | Enviro Tech Chemical Services, Inc. | Algaecidal compositions for water treatment and method of use thereof |
-
2013
- 2013-09-17 CN CN201310424955.5A patent/CN103518698B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4847089A (en) * | 1986-07-16 | 1989-07-11 | David N. Kramer | Cleansing and distinfecting compositions, including bleaching agents, and sponges and other applicators incorporating the same |
| CN102550388A (en) * | 2012-01-06 | 2012-07-11 | 上海海洋大学 | Method for removing green algae in the middle and late culture stages of Porphyra yezoensis |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110235773A (en) * | 2019-07-26 | 2019-09-17 | 国家海洋环境监测中心 | Seaweed raft frame green alga preventing control method based on micro- traditional font library abatement of aestivating |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103518698A (en) | 2014-01-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Redmond et al. | New England seaweed culture handbook: nursery systems | |
| Redmond et al. | New England seaweed culture handbook | |
| Bezerra et al. | Cultivation of the red seaweed Gracilaria birdiae (Gracilariales, Rhodophyta) in tropical waters of northeast Brazil | |
| CN101822271B (en) | Acidizing fluid for green algae and disease infected cell in porphyra haitanensis cultivation and treating method thereof | |
| CN104620832B (en) | Ancient Cacumen Platycladi cuttage breeding method | |
| CN104813939B (en) | Method for constructing lotus regeneration system | |
| Jothinayagi et al. | Effect of seaweed liquid fertilizer of Sargassum wightii on the growth and biochemical characteristics of Abelmoschus esculentus (L.) Medikus | |
| CN105104164B (en) | Sargassum fusifome quality breeding method | |
| CN102783415B (en) | Method for conservation in vitro of cassava germplasm resources with stability and high efficiency | |
| Yarish et al. | Gracilaria culture handbook for new England | |
| CN103518698B (en) | The sweep-out method of green alga in a kind of laver culture process | |
| CN102550388B (en) | Method for removing green algae in the middle and late culture stages of Porphyra yezoensis | |
| CN105016524A (en) | Method for removing floating cladophora | |
| CN107996242A (en) | A kind of Moringa container seedling culture method | |
| CN112889849B (en) | Composite plant extracts, compositions and methods of use for controlling the growth of cladophora | |
| CN104957063B (en) | River crab master supports the ecological prevention method of pond moss | |
| Kimathi et al. | Growth rates of Eucheuma denticulatum and Kappaphycus alvarezii (Rhodophyta; Gigartinales) cultured using modified off-bottom and floating raft techniques on the Kenyan coast | |
| CN109496826A (en) | Huanghai Sea green tide preventing control method based on overwintering micro- traditional font library abatement | |
| CN105706869B (en) | A kind of horse-tail takes root the method for nursery | |
| CN106259319A (en) | A kind of multifunctional slow-release algicide | |
| CN110178726A (en) | A kind of root media and weeping willow tissue culture and rapid propagation method for weeping willow tissue-culturing rapid propagation | |
| CN204762659U (en) | Laver cultivation raft frame | |
| CN104957025B (en) | It is a kind of to prevent and treat the method that green alga grows nonparasitically upon another plant on seaweed cultivation raft frame | |
| Rød | Sori disinfection in cultivation of Saccharina latissima: Evaluation of chemical treatments against diatom contamination | |
| CN104926000A (en) | Method for rapidly clearing away floating green alga spirogyra |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |