CN103518698A - Method for removing green alga in nori cultivation process - Google Patents
Method for removing green alga in nori cultivation process Download PDFInfo
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- CN103518698A CN103518698A CN201310424955.5A CN201310424955A CN103518698A CN 103518698 A CN103518698 A CN 103518698A CN 201310424955 A CN201310424955 A CN 201310424955A CN 103518698 A CN103518698 A CN 103518698A
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- green alga
- sodium percarbonate
- algicide
- laver
- sweep
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Abstract
The invention relates to a method for restraining green alga growth, in particular to a method for removing green alga in the nori cultivation process. Firstly, algaecide is prepared, wherein solid sodium percarbonate is dissolved in deionized water, so that sodium percarbonate treating fluid is prepared, and the pH value of the treating fluid is between 9 and 10; the prepared algaecide is directly sprayed on green alga bodies attached to a net curtain, a whole raft frame and a whole mooring rope in the nori cultivation process 2 hours before a flood tide and the algaecide is sprayed once every 1 to 3 months. According to the method for removing the green alga in the nori cultivation process, the sodium percarbonate serves as the algaecide and is free of toxin, odor and pollution, high in water solubility, convenient to prepare, free of harm to the environment and free of biological accumulation. The method for removing the green alga in the nori cultivation process is very simple and convenient to achieve, the use cost is low, rinse is not required, and the method is slightly influenced by natural conditions and can be used in a large scale. Three days later after the algaecide is sprayed, the removal rate of the green alga is 100%, and the growth of the nori is not influenced.
Description
Technical field
The present invention relates to a kind of method that suppresses chlorella growth, particularly the sweep-out method of the green alga in a kind of laver culture process.
Background technology
The cultivation of laver has long history in China, is one of industry traditional and more representative in China Water product aquaculture, and industry size is only second to sea-tangle and occupies second in the middle of algae.Occupation rate in laver market, the Cultivation of Porphyra In China Ye world constantly rises, especially the later stage nineties presents significant growing trend especially, China in 2000 surmounts Japan and suddenly becomes the first in the world laver culture big country, China laver culture Liang Zhan world cultivation total amount 65% in 2009, has initially increased by 54% than cultivation in 1971.
Laver growth cycle is divided into grows seedlings, collects seedling, nets training (putting into sea area) and the four-stage of gathering.And in the net training stage of laver, green alga will badly influence the growth of laver.
Annual late April starts, the middle ten days upper to September planting vegetables of thallus of porphyra ripe (carpospore cyst and anthreid form) to be placed in to the container that fills clean sea water, after diffusing carpospore, be attached on clam shell or oyster shell, enter filamentous and cultivate, grow seedlings the stage.The seawater of growing seedlings is generally the clean sea water after being exposed to the sun and precipitating, so only have the spore of laver can be attached on lace curtaining, green alga can affect laver seedling raising process hardly; By ripe filamentous, conchospore collects on preprepared laver lace curtaining, and this stage is the stage of collecting seedling, and green alga does not affect the process of collecting seedling yet; In 9 months, the last ten-days period laver adopted after seedling and lace curtaining can have been put into sea area, now, the green alga spore existing in sea area just can be attached on lace curtaining, raft frame and hawser.Mid-October green alga raised growth, because sea area exists a large amount of green alga spores, it can competitiveness anchor on lace curtaining, through the growth of 1 month, now on lace curtaining, growth had the long green alga of 10cm, and biomass is huge, and laver is only seedling state, its length is only 500 μ m left and right.So the raised growth of green alga, seriously hinders the normal growth of laver.
Traditional green alga sweep-out method is: cold net method, lace curtaining is transported back on the bank, and put freezer into about 20 days; Seasoning, takes back lace curtaining on the bank, is placed on spacious place and is exposed to the sun one day.But these traditional methods can only partly be removed the green alga on lace curtaining, and the green alga on raft frame and hawser also can not be eliminated.Even if treated lace curtaining, puts into the green alga that also can regrow out behind sea area again, have a strong impact on the percentage of product of laver, the income of laver is caused to huge loss.
In prior art, also useful acid-treated method is removed the green alga growing in laver culture process, as University Of Ningbo discloses pickling agent and the processing method of Porphyra haitanensis cultivation green algae and the cell of catching an illness, publication number is 101822271A, when the shortcoming of the method is preparating acid treatment fluid, not only can form harm to health, but also can cause the secondary pollution of ocean.Human body contacts its steam or smog, can cause acute poisoning, occurs eye conjunctivitis, and nose and oral mucosa have burn feeling, gingiva bleeding gingival hemorrhage, tracheitis etc.Hydrochloric acid has harm to environment, to water body and soil, can pollute, and the hydrochloric acid of low concentration also can kill Marine Planktonic animals and plants, destroys the ecological balance, and hydrochloric acid belongs to hazardous chemical, has environmental risk in storage process.Although, in this patent, point out that acid treatment reaches 90% to the clearance rate of green alga, can cause larger impact to laver, thallus of porphyra diminishes, and turns to be yellow etc., affects the quality of laver.Equally, the method also can not prevent that lace curtaining from putting into green alga continued growth behind sea area.
In laver culture, conventionally also adopt containing the agricultural chemicals of plant growth inhibitor and remove green alga, agricultural chemicals is a class bioactivator, it is again a class chemically reactive substance, after distribution, can residue in environment, biocenological composition and variation in specific environment be caused to certain impacts, or interact with some other material or the object in environment, diffusion profile in specific environment, causes adverse consequences to the benign development of the ecosystem.
So algae removal agent or the growth inhibitor of high security have the very high market demand.
Summary of the invention
The sweep-out method that the object of this invention is to provide the green alga in a kind of laver culture process, this method adopts SODIUM PERCARBONATE as algicide, and can remove effectively and quickly green alga does not affect again the normal growth of laver simultaneously, and can not pollute environment yet, do not need rinsing, simple, easy to operate.
Object of the present invention can be achieved through the following technical solutions:
A sweep-out method for green alga in process, its step comprises:
In flood tide first 2 hours, by algicide be directly sprayed in laver culture be attached on the green alga frond on lace curtaining, on whole raft frame and whole piece hawser, the sprinkling frequency of this algicide is 1-3 month/time; Preferably, the sprinkling frequency of this algicide is 2 months/times;
The compound method of described algicide is, solid SODIUM PERCARBONATE is dissolved in deionized water, is mixed with SODIUM PERCARBONATE treatment fluid, and the pH value of this treatment fluid is 9-10;
The valid density of the SODIUM PERCARBONATE in described SODIUM PERCARBONATE treatment fluid is 40-150mmol/L.Preferably, the valid density of the SODIUM PERCARBONATE in described SODIUM PERCARBONATE treatment fluid is 40-100mmol/L.
Sprinkling amount on the green alga frond of described algicide on lace curtaining is 1-6mL/cm
2, the sprinkling amount of this algicide on whole raft frame and whole piece hawser is 120-170mL/ rice.Preferably, the sprinkling amount on the green alga frond of described algicide on lace curtaining is 2-3mL/cm
2, the sprinkling amount of this algicide on whole raft frame and whole piece hawser is 150mL/ rice.
Described laver is porphyra yezoensis.
Compared with prior art, beneficial effect of the present invention is:
1, the present invention adopts SODIUM PERCARBONATE as algicide, removes the green alga growing in laver culture process, and that SODIUM PERCARBONATE has is nontoxic, odorless, the advantage such as pollution-free, chemical property is more stable, is convenient to transportation, water-soluble strong, facilitate adapted, environmental sound and inanimate object cumulative bad.
2, the green alga sweep-out method in laver culture process provided by the invention is very simple, convenient, and use cost is low, without rinsing, is subject to effect of natural conditions less, can use on a large scale.Algicide sprayed after 3 days, and the clearance rate of green alga is 100%, and the growth of laver is not formed to impact.
Accompanying drawing explanation
Fig. 1 is the growth rate comparison diagram that sprays the flat Enteromorpha sample after SODIUM PERCARBONATE treatment fluid.
Fig. 2 is the chlorophyll fluorescence parameters maximum amount suboutput comparison diagram that sprays the flat Enteromorpha sample after SODIUM PERCARBONATE treatment fluid.
Fig. 3 sprays after SODIUM PERCARBONATE treatment fluid, the photosynthetic rate after standing 120min and the variation diagram of P/R.
Embodiment
Below in conjunction with embodiment, the invention will be further described:
Embodiment 1
In the present embodiment, choose porphyra yezoensis culture zone, Jiangsu Rudong county raft frame as experimental subjects, concrete steps are as follows:
(1), the preparation of algicide: solid SODIUM PERCARBONATE is dissolved in deionized water, is mixed with SODIUM PERCARBONATE treatment fluid, pH value is 9.5, and the valid density of the SODIUM PERCARBONATE in this treatment fluid is 100mmol/L.
(2), in flood tide first 2 hours, convenient operation when porphyra yezoensis raft frame all surfaces.Algicide in step (1) is sprayed directly on the green alga frond being attached on lace curtaining, sprinkling amount is 2-3mL/cm
2, algicide directly to be sprayed on whole raft frame and whole piece hawser, sprinkling amount is 150mL/ rice.The sprinkling frequency of this algicide is 2 months/times.In porphyra yezoensis breeding process, fisherman almost can plunge into the commercial sea and carry out arrangement and the maintenance of laver lace curtaining every day, so, when plunging into the commercial sea arrangement and safeguarding, can select lace curtaining, raft frame and hawser to be carried out to spraying operation in batches, be subject to effect of natural conditions less, simple to operate, can use on a large scale.
Algicide is sprayed to green alga frond after upper 3 day, and frond is all dead, and lethality reaches 100%, in natural tidal phenomena, under the physical action of dead green alga in marine stream, fracture enters sea area, reaches the object of removing green alga, and the growth of porphyra yezoensis is not formed to impact.
Experiment material: fresh, healthy flat Enteromorpha sample is adopted back in sea area and obtained after laboratory cultures in Jiangsu Rudong county.
In order to detect the inhibition of algicide provided by the invention to chlorella growth, by Enteromorpha, be that detected object is tested:
SODIUM PERCARBONATE dissolution of solid, in distilled water, is mixed with to the treatment fluid that SODIUM PERCARBONATE valid density is 40-50mmol/L, is placed in watering can standby.Because the Enteromorpha quantity of cultivating in laboratory is fewer, do not have the Enteromorpha quantity in actual field many, so in the treatment fluid of preparation, SODIUM PERCARBONATE valid density is that 40-50mmol/L just can suppress the normal growth of Enteromorpha.
The flat Enteromorpha sample of selecting some complete health, sucks surface moisture, is divided into 6 groups, and every group is about 2cm
2with the above-mentioned SODIUM PERCARBONATE treatment fluid preparing, evenly spray wherein 3 groups, be labeled as experimental group, every group of sprinkling amount is 4-6mL, and all the other 3 groups as a control group, with distilled water, sprays, respectively after standing 30min, 60,120min with seawater flushing, measure chlorophyll fluorescence parameters and the photosynthetic oxygen evolution speed of frond, and then flat Enteromorpha sample is put into blake bottle renewal cultivation, observe the frond state variation of every day.
Test result shows:
The flat Enteromorpha sample grown rate of 1, spraying through SODIUM PERCARBONATE treatment fluid obviously declines, after processing 30,60,120min, more blank group has declined respectively 57.96%, 28.50%, 84.50%, specifically as shown in Figure 1, as can be seen from Figure, SODIUM PERCARBONATE treatment fluid sprays after flat Enteromorpha sample, standing 120min, can make it substantially stop growing.
2, chlorophyll fluorescence parameters maximum amount suboutput Fv/Fm is along with processing time lengthening is the trend of successively decreasing gradually, after the sprinkling of SODIUM PERCARBONATE treatment fluid standing 120min, although can not be lethal rapidly, but Fv/Fm is than 50% left and right that declined of the blank group under the same terms, photosynthetic activity reduces, specifically as shown in Figure 2.
3, experiment combination control group is processed through spraying, and respectively after standing 120min, more blank group of the photosynthetic oxygen evolution speed of experimental group frond declines to a great extent, and only has 1/5 of blank group; The respiratory rate of experimental group frond obviously raises, the P/R(photosynthetic oxygen evolution speed/respiratory rate of experimental group) value has approached 1, lower than the normal P/R value (normal P/R value=4) of green alga, specifically as shown in Figure 3, the photosynthesis of illustrative experiment group Enteromorpha is by severe inhibition.After one week, the death that bleaches of experimental group Enteromorpha root, shows that SODIUM PERCARBONATE has remarkable inhibition to the growth of green alga frond.
The above is preferred embodiment of the present invention, but the present invention should not be confined to the disclosed content of this embodiment.So every, do not depart from the equivalence completing under spirit disclosed in this invention or revise, all falling into the scope of protection of the invention.
Claims (7)
1. a sweep-out method for the green alga in laver culture process, its step comprises:
In flood tide first 2 hours, by algicide be directly sprayed in laver culture be attached on the green alga frond on lace curtaining, on whole raft frame and whole piece hawser, the sprinkling frequency of this algicide is 1-3 month/time;
The compound method of described algicide is, solid SODIUM PERCARBONATE is dissolved in deionized water, is mixed with SODIUM PERCARBONATE treatment fluid, and the pH value of this treatment fluid is 9-10.
2. the sweep-out method of the green alga in laver culture process according to claim 1, is characterized in that: the valid density of the SODIUM PERCARBONATE in described SODIUM PERCARBONATE treatment fluid is 40-150mmol/L.
3. the sweep-out method of the green alga in laver culture process according to claim 2, is characterized in that: the valid density of the SODIUM PERCARBONATE in described SODIUM PERCARBONATE treatment fluid is 40-100mmol/L.
4. the sweep-out method of the green alga in laver culture process according to claim 1, is characterized in that: the sprinkling amount on the green alga frond of algicide on lace curtaining is 1-6mL/cm
2, the sprinkling amount of algicide on whole raft frame and whole piece hawser is 120-170mL/ rice.
5. the sweep-out method of the green alga in laver culture process according to claim 4, is characterized in that: the sprinkling amount on the green alga frond of algicide on lace curtaining is 2-3mL/cm
2, the sprinkling amount of algicide on whole raft frame and whole piece hawser is 150mL/ rice.
6. the sweep-out method of the green alga in laver culture process according to claim 1, is characterized in that: the sprinkling frequency of described algicide is 2 months/times.
7. according to the sweep-out method of the green alga in the laver culture process described in any one in claim 1-6, it is characterized in that: described laver is porphyra yezoensis.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107262439A (en) * | 2017-06-28 | 2017-10-20 | 中国科学院海洋研究所 | A kind of method for removing miscellaneous algae Enteromorpha on cultivation lace curtaining |
| CN109496826A (en) * | 2018-11-13 | 2019-03-22 | 国家海洋环境监测中心 | Huanghai Sea green tide preventing control method based on overwintering micro- traditional font library abatement |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110235773A (en) * | 2019-07-26 | 2019-09-17 | 国家海洋环境监测中心 | Seaweed raft frame green alga preventing control method based on micro- traditional font library abatement of aestivating |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107262439A (en) * | 2017-06-28 | 2017-10-20 | 中国科学院海洋研究所 | A kind of method for removing miscellaneous algae Enteromorpha on cultivation lace curtaining |
| CN109496826A (en) * | 2018-11-13 | 2019-03-22 | 国家海洋环境监测中心 | Huanghai Sea green tide preventing control method based on overwintering micro- traditional font library abatement |
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