CN103435395A - Drip-irrigation type salt stress-alleviating and disease-preventing biological bacterial fertilizer and preparation method thereof - Google Patents
Drip-irrigation type salt stress-alleviating and disease-preventing biological bacterial fertilizer and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a drip-irrigation type salt stress-alleviating and disease-preventing biological bacterial fertilizer and a preparation method thereof. The drip-irrigation type salt stress-relieving and disease-preventing biological bacterial fertilizer comprises nutrition carriers, additives, microelements and functional microorganisms, wherein the nutrition carriers comprise two or more of humic acid, glutamic acid, urea, corn flour, potassium sulphate, potassium chloride and diammonium hydrogen phosphate; the additives comprise two or more of bentonite, sodium carboxymethyl cellulose, sodium alginate an polyvinyl alcohol; the microelements comprise one or two of boric acid an ammonium molybdate; and the functional microorganisms is formed by combining salt stress-alleviating growth-promoting bacteria and disease-preventing growth-promoting bacteria, wherein the salt stress-alleviating growth-promoting bacteria is one or two selected from pseudomonas putida and azotobacter chroococcum; and the disease-preventing growth-promoting bacteria is one or two selected from bacillus subtilis and bacillus megatherium. The biological bacterial fertilizer is in a liquid dosage form, has relatively high effective viable count and relatively long quality guarantee period. The effective viable count of the biological bacterial fertilizer is higher than or equal to 10<10> cfu/mL; and total nutrient content of (N+P2O5+K2O) is higher than or equal to 12%.
Description
Technical field
What the present invention relates to is microorganism field, specifically a kind of drip irrigation formulation solution salt diseases prevention bio-bacterial manure and preparation method thereof.
Background technology
Xinjiang is located in the NORTHWEST CHINA inland, because strong evaporation from land and crop transpiration impel the salinity Upward Migration in underground water, thereby causes the accumulation of salinity in soil; Simultaneously, unsuitable agricultural irrigation and water conservancy projects, cause the soil salinization, thereby affect agricultural planting.Xinjiang wherein Jin80Ge county (city) all faces serious soil salinization problem, particularly outstanding with Southern Xinjiang, as the salinification of Kaxgar Prefecture, ploughs and accounts for 48.72% of regional cultivated area.In recent years, various types of Secondary Salines are day by day serious, cause that crop is short of seedling, disconnected ridge, production declining, seriously restricted the development of Xinjiang agriculture.Therefore, need to wherein utilize the bio-technology improvement saltings from the solution of the new angle research soil salinization, the alleviation plant salt is coerced day by day and is come into one's own.
In addition, because Xinjiang low temperature in early spring, range of temperature are large, often cause that seedling blight generally occurs, cause harm serious.The general sickness rate of cotton seedling root rot caused by damping-off cause of disease Rhizictonia solani Kuhn is 27%-75%, and serious reaches more than 90%, causes the disconnected ridge that is short of seedling, and greatly affects crop yield and quality.Chemical prevention is main prophylactico-therapeutic measures, but has contaminate environment, easily causes pesticide residue, easily makes pathogenic bacteria develop immunity to drugs, and destroys the problems such as the eubiosis, usually is difficult to the prevention effect that reaches satisfied.Therefore, need to find new way disease control is studied, wherein biological control has become study hotspot preventing and treating aspect soil-borne disease.
Recent study shows, beneficial rhizosphere microorganisms can promote growing of crop, improves output, more can help crop to tolerate the adverse environmental factors such as some are saline and alkaline, arid, heavy metal.Therefore, utilize plant growth-promoting rhizobacteria to help crop to improve soil salinization tolerance, the microbial preparation that exploitation has good growth-promoting functions receives publicity day by day.In addition, at plant rhizosphere, exist many beneficial rhizosphere microorganisms that crop pest had to good control effect, and can, by directly with the effect of indirect two aspects, Plant diseases being controlled, increase plant resistance and growth potential simultaneously.Therefore, adopting biological method to address these problems is current development trend.
Yet, also more single for the microbial fertilizer function at present, and the screening study of bacterial strain and nutrition carrier system also lacks.In addition, still belong at present blank for the research of the microbial fertilizer of drip irrigation model application, and similar competitive products is abroad also not yet arranged.The solution salt diseases prevention bio-bacterial manure of a kind of drip irrigation formulation of the present invention, be adapted to the Xinjiang climatope and be suitable for the solution salt disease-proof functions microbial fertilizer product of applying under the drip irrigation pattern, apply mode by drip irrigation bacterial manure is applied to plant rhizosphere, the viable bacteria body is more easily grown surely in plant rhizosphere, what promote thalline and crop makes effect mutually, effectively improve thalline and surely grow rate, survival volume and long-lasting at crop rhizosphere, give full play to the usefulness of thalline, solved the problems such as the ubiquitous long-lasting of microbial fertilizer was poor in the past, stability is low, effect is not obvious.Therefore, the bio-bacterial manure that is suitable for the Under-Mulch-Drip Irrigation in Xinjiang pattern and the preparation method of Patent exploitation of the present invention, will have larger application potential and wide market outlook.
Summary of the invention
The object of the present invention is to provide that a kind of living bacteria count is high, cost is low, be applicable to the application of drip irrigation cropping pattern, plant is had and alleviate salt stress, prevent and treat disease and promote bio-bacterial manure of the effects such as growth and preparation method thereof.
The objective of the invention is to be achieved through the following technical solutions.
A kind of drip irrigation type solution salt diseases prevention bio-bacterial manure of the present invention and preparation method thereof, it comprises nutrition carrier, auxiliary agent, trace element and functional microorganism; Wherein, effective bacterial content>=10 in bio-bacterial manure
10cfu/ml, total nutrient (N+P
2o
5+ K
2o)>=12%, and preserve effective viable bacteria content>=10 in bacterial manure of the present invention half a year
8cfu/ml.
Described a kind of drip irrigation formulation solution salt diseases prevention bio-bacterial manure is characterized in that comprising: take the fertilizer oeverall quality as 100%:
Nutrition carrier 10%-40%;
Auxiliary agent 5%-15%;
Trace element 1%-2%;
Microbial fermentation solution 50%-80%.
Described nutrition carrier comprises two or more in humic acid, L-glutamic acid, urea, Semen Maydis powder, vitriolate of tartar, Repone K, Secondary ammonium phosphate; Auxiliary agent comprises two or more in wilkinite, Xylo-Mucine, sodium alginate, polyvinyl alcohol; Trace element comprises one or both in boric acid, ammonium molybdate.
Described functional microorganism comprises Salte stress-relieving and growth-promoting bacterium and disease prevention growth-promoting bacterium, wherein the Salte stress-relieving and growth-promoting bacterium comprises one or both in pseudomonas putida (Pseudomonas putida preserving number ATCC17485), azotobacter chroococcum (Azotobacter chroococcum preserving number is ACCC10098), and described two kinds of bacterial strains are purchased from Chinese agriculture microbial strains preservation administrative center.These microorganisms can reduce under salt stress plant to Na
+the absolute absorption amount, increase K
+, Ca
2+and Fe
2+absorption, effectively remove secondary salinization to the coercing of crop, there is molten phosphorus and nitrogen fixing capacity, the absorption of strong crop to nutritive element; The disease prevention growth-promoting bacterium comprises subtilis (Bacillus subtilis-NCD-2 preserving number is CGMCC1019), one or both in bacillus cereus (Bacillus cereus preserving number is CGMCC4991), described two kinds of bacterial strains are purchased from respectively China Committee for Culture Collection of Microorganisms's common micro-organisms center.These microorganisms, to the crops seedling stage disease, as root rot, blight and verticillium evil etc. have good prevention, preventive and therapeutic effect, can promote plant strain growth to reach the absorption to nutritive substance.
A kind of drip irrigation formulation solution salt diseases prevention bio-bacterial manure of the present invention and preparation method thereof, the specific implementation step is as follows:
Step 1, by the activation of the functional microorganism of cryopreservation, liquid culture seed liquor, standby; Comprising:
(1) preparation of NA substratum
By NaCl (5.0g), peptone (10.0g), extractum carnis (3.0g), H
2o (1000ml) is mixed, configuration NA substratum; If solid NA substratum, add 1.8% agar; Sterilizing, standby;
(2) activation of bacterial strain
The beneficial microorganism of cryopreservation is rule on the NA substratum, 28-32 ℃ of lower constant temperature culture 2-3 days; Picking list bacterium colony, streak culture on new NA solid medium, so 2-3 time;
(3) cultivation of functional microorganism
Functional microorganism by after activation, be linked into Semen Maydis powder, dregs of beans liquid nutrient medium (Semen Maydis powder 50g, bean cake powder 16g, K
2hPO
42g, MnSO
40.2g, NaCl5g, H
2o1000ml), in, under 28-32 ℃, the condition of 150-220rpm, enlarged culturing to microbe population reaches 10
12more than cfu/ml, be cultured to logarithmic phase, standby.
Step 2, get the nutrition carrier that constituent mass per-cent is respectively 10%-40%, the auxiliary agent of 5%-15%, and the trace element of 1%-2% fully mixes; Sterilizing, standby;
Step 3, mix the bacterium liquid of step 1 with nutrition carrier, auxiliary agent, the trace element of step 2, wherein, the mass percent of functional microorganism is 50%-80%, obtains a kind of drip irrigation formulation solution salt diseases prevention bio-bacterial manure.
A kind of solution salt diseases prevention bio-bacterial manure made through above-mentioned steps, its finished product index is as follows:
pH：6.8～7.6；
Total nutrient (N+P
2o
5+ K
2o)>=12%;
Living bacteria count in bacterial manure is greater than 10
10cfu/ml;
After the room temperature lower seal stores half a year, the living bacteria count in bacterial manure can be greater than 10
8cfu/ml;
Miscellaneous bacteria rate≤10%;
Solid substance particle diameter≤0.1mm;
Compare the technological innovation of patent of the present invention and beneficial effect with existing patent of invention technology:
1, bio-bacterial manure of the present invention has the effects such as disease such as the salt stress of alleviation, control root rot in seedling stage, blight, and can promote seed germination and plant growth, increases crop yield, improves crop quality, reduces agriculture production cost;
2, bio-bacterial manure of the present invention belongs to liquid dosage form, is suitable for applying under the Under-Mulch-Drip Irrigation in Xinjiang cropping pattern, by drip irrigation, bacterial manure is applied to crop rhizosphere, effectively improve thalline and surely grow quantity, give full play to the thalline effect, realize being easy to surely grow technological breakthrough and innovation that effect guarantees;
3, bio-bacterial manure of the present invention, original screening organotrophy carrier and auxiliary agent carrier system technology, for thalline provides the required various nutrients of growing, guarantee that living bacteria count is high, long quality-guarantee period and good stability.Solved the problem of bio-feritlizer result of use poor stability in the past.
The accompanying drawing explanation
Fig. 1 is for separating the design sketch of the cotton seedling of salt diseases prevention bio-bacterial manure drip irrigation;
Fig. 2 is the blank design sketch.
Embodiment
A kind of drip irrigation formulation solution salt diseases prevention bio-bacterial manure of the present invention, using two or more in humic acid, L-glutamic acid, urea, Semen Maydis powder, vitriolate of tartar, Repone K and Secondary ammonium phosphate as nutrition carrier, using two or more in wilkinite, Xylo-Mucine, sodium alginate, polyvinyl alcohol as auxiliary agent, using one or both in boric acid, ammonium molybdate as trace element, with the fermented liquid of each one or both bacterial strains in functional microorganism Salte stress-relieving and growth-promoting bacterium and biocontrol microorganisms, carry out the composite of each composition and separate salt diseases prevention bio-bacterial manure with exploitation.
For the characteristic that absolutely proves the technical program and the mode of implementing the technical program, below provide embodiment, but the present invention is not limited to this.
Preparation Example 1
The mass ratio of each composition is as follows:
Each component in nutrition carrier, auxiliary agent, trace element is mixed according to aforementioned proportion, and under 121 ℃ of conditions, sterilizing is 20 minutes, standby.Under aseptic condition, prior cultured functional microorganism is added in above-mentioned solution according to aforementioned proportion, obtain the liquid dosage form bio-bacterial manure.Wherein, the pH value is 6.8～7.6, total nutrient N+P
2o
5+ K
2o>=12.1%, the living bacteria count in bacterial manure is greater than 10
10cfu/ml, and preserve the living bacteria count in bacterial manure after half a year under room temperature and can reach 10
8cfu/ml, miscellaneous bacteria rate≤10%.
Preparation Example 2
The mass ratio of each composition is as follows:
Each component in nutrition carrier, auxiliary agent, trace element is mixed according to aforementioned proportion, and under 121 ℃ of conditions, sterilizing is 20 minutes, standby.Under aseptic condition, prior cultured functional microorganism is added in above-mentioned solution according to aforementioned proportion, obtain the liquid dosage form bio-bacterial manure.Wherein, the pH value is 6.8～7.6, total nutrient N+P
2o
5+ K
2o>=13.06%, the living bacteria count in bacterial manure is greater than 10
10cfu/ml, and preserve the living bacteria count in bacterial manure after half a year under room temperature and can reach 10
8cfu/ml, miscellaneous bacteria rate≤10%.
Preparation Example 3
The mass ratio of each composition is as follows:
Each component in nutrition carrier, auxiliary agent, trace element is mixed according to aforementioned proportion, and under 121 ℃ of conditions, sterilizing is 20 minutes, standby.Under aseptic condition, prior cultured functional microorganism is added in above-mentioned solution according to aforementioned proportion, obtain the liquid dosage form bio-bacterial manure.Wherein, the pH value is 6.8～7.6, total nutrient N+P
2o
5+ K
2o>=14.02%, the living bacteria count in bacterial manure is greater than 10
10cfu/ml, and preserve the living bacteria count in bacterial manure after half a year under room temperature and can reach 10
8cfu/ml, miscellaneous bacteria rate≤10%.
Preparation Example 4
The mass ratio of each composition is as follows:
Each component in nutrition carrier, auxiliary agent, trace element is mixed according to aforementioned proportion, and under 121 ℃ of conditions, sterilizing is 20 minutes, standby.Under aseptic condition, prior cultured functional microorganism is added in above-mentioned solution according to aforementioned proportion, obtain the liquid dosage form bio-bacterial manure.Wherein, the pH value is 6.8～7.6, total nutrient N+P
2o
5+ K
2o>=14.98%, the living bacteria count in bacterial manure is greater than 10
10cfu/ml, and preserve the living bacteria count in bacterial manure after half a year under room temperature and can reach 10
8cfu/ml, miscellaneous bacteria rate≤10%.
In order to investigate the cotton growth situation by solution salt diseases prevention bio-bacterial manure drip irrigation of the present invention, carry out field test under the envrionment conditions of land for growing field crops, prior to seeding leading ion level experimental field to be measured, the total salt content of anthropogenic soil is 0.3-0.7%.
Test example 1
By the solution salt diseases prevention bacterial manure of Preparation Example 3 of the present invention, sowing in the usual way, dilute 10-20 by the present invention and doubly with water droplet, fill with every mu of consumption 5-10 kilogram, applies fertilizer 3-4 time.After emerging 30 days, investigation seeding ratio, sickness rate, and calculate disease index and protection effect, in Table 1.
Table 1 separates that salt diseases prevention bio-bacterial manure is emerged to cotton and the influence of sprout term disease
Result from table 1, cotton seeds is after processing by solution salt diseases prevention bio-bacterial manure drip irrigation of the present invention, and seedling rate and protection effect all are significantly improved, and sickness rate reduces.
Test example 2
Separate the impact of salt diseases prevention bio-bacterial manure on vegetable lamb biomass (plant height, fresh weight, dry weight, individual plant leaf area, leaf area index) in order to investigate, in Table 2.
Table 2 is separated the influence of salt diseases prevention bio-bacterial manure to cotton biomass and output
Result from table 2, cotton seeds is after processing by solution salt diseases prevention bio-bacterial manure drip irrigation of the present invention, and vegetable lamb biomass (plant height, fresh weight, dry weight, individual plant leaf area, leaf area index) all has significant improvement.
Test example 3
Separate the impact of salt diseases prevention bio-bacterial manure on the volume increase (Bolls per plant, Dan Lingchong, mu strain number, seed cotton yield) of cotton in order to investigate, in Table 3.
Table 3 is separated salt diseases prevention bio-bacterial manure to the Yield Increase In Cotton effect
Result from table 3, cotton seeds is after processing by solution salt diseases prevention bio-bacterial manure drip irrigation of the present invention, and the Bolls per plant of vegetable lamb, Dan Lingchong, mu strain number, seed cotton yield all obviously increase.
Claims (8)
1. drip irrigation formulation solution salt diseases prevention bio-bacterial manure and preparation method thereof, it is characterized in that: it comprises nutrition carrier, auxiliary agent, trace element and functional microorganism.The bio-bacterial manure of preparation has the salt stress of alleviation, prevents and treats the effects such as soil-borne disease and promotion plant growth, effective bacterial content>=10 in bio-bacterial manure
10cfu/ml, total nutrient (N+P
2o
5+ K
2o)>=12%, solid substance particle diameter≤0.1mm, and preserve after half a year in bacterial manure effectively viable bacteria content>=10
8cfu/ml.
2. according to described a kind of drip irrigation formulation solution salt diseases prevention bio-bacterial manure of claims 1 and preparation method thereof, it is characterized in that comprising: take the fertilizer oeverall quality as 100%:
Nutrition carrier 10%-40%;
Auxiliary agent 5%-15%;
Trace element 1%-2%;
Microbial fermentation solution 50%-80%.
3. according to claim 1,2 described a kind of drip irrigation formulation solution salt diseases prevention bio-bacterial manures and preparation method thereof, it is characterized in that: described nutrition carrier comprises two or more in humic acid, L-glutamic acid, urea, Semen Maydis powder, vitriolate of tartar, Repone K, Secondary ammonium phosphate; Auxiliary agent comprises two or more in wilkinite, Xylo-Mucine, sodium alginate, polyvinyl alcohol; Trace element comprises one or both in boric acid, ammonium molybdate.
4. a kind of drip irrigation formulation solution salt diseases prevention bio-bacterial manure according to claim 1 and preparation method thereof, it is characterized in that: described functional microorganism comprises Salte stress-relieving and growth-promoting bacterium and disease prevention growth-promoting bacterium, and wherein the Salte stress-relieving and growth-promoting bacterium comprises a kind of in pseudomonas putida (Pseudomonas putida preserving number ATCC17485), azotobacter chroococcum (Azotobacter chroococcum preserving number is ACCC10098) or two kinds; The disease prevention growth-promoting bacterium comprises one or both in subtilis (Bacillus subtilis-NCD-2 preserving number is CGMCC1019), bacillus cereus (Bacillus cereus preserving number is CGMCC4991).
5. a kind of drip irrigation formulation solution salt diseases prevention bio-bacterial manure according to claim 4 and preparation method thereof, it is characterized in that: the volume ratio of each functional microorganism fermented liquid is that pseudomonas putida: azotobacter chroococcum: subtilis: bacillus cereus is 1～2: 0～2: 1～3: 0～3.
6. drip irrigation formulation solution salt diseases prevention bio-bacterial manure and preparation method thereof is characterized in that according to the following steps or order is configured:
1. after two or more in nutrition carrier being dissolved in water, then mix with two or more the aqueous dispersions in auxiliary agent, and add one or both in trace element, 121 ℃ of sterilizing 20min, standby.
2. nutrition carrier, auxiliary agent, trace element and functional microorganism fermented liquid mechanical stirring in inciting somebody to action 1. in the conditioned reaction still are mixed to get finished product.
7. a kind of drip irrigation formulation solution salt diseases prevention bio-bacterial manure according to claim 1 and preparation method thereof is characterized in that: separating salt diseases prevention bio-bacterial manure is a kind of liquid dosage form, effective bacterial content>=10 in bio-bacterial manure
10cfu/ml, total nutrient (N+P
2o
5+ K
2o)>=12%, solid substance particle diameter≤0.1mm, and preserve after half a year in bacterial manure effectively viable bacteria content>=10
8cfu/ml.
8. a kind of drip irrigation formulation solution salt diseases prevention bio-bacterial manure according to claim 7 and preparation method thereof, it is characterized in that: the bio-bacterial manure of exploitation is applicable to the crops such as cotton, processing tomato and wheat under the drip irrigation cropping pattern, effectively alleviate the soil salinization, control soil-borne disease in seedling stage, promotion plant growth, obvious effect of increasing production, and the drip irrigation application process is without latch up phenomenon.
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104262057A (en) * | 2014-09-29 | 2015-01-07 | 石河子大学 | Rhizosphere acid fertilizer and preparation method thereof |
| CN104671997A (en) * | 2015-01-30 | 2015-06-03 | 姜明光 | Biological liquid compound fertilizer containing monosilicic acid, N, P and K |
| CN104817363A (en) * | 2014-10-09 | 2015-08-05 | 北京绿可欣生物科技有限公司 | Humic acid water-soluble fertilizer, and preparation and application methods thereof |
| CN105001017A (en) * | 2015-06-16 | 2015-10-28 | 西南科技大学 | Preparation method for desert soil micro drip irrigation microbial bacterial manure |
| CN106748542A (en) * | 2017-02-13 | 2017-05-31 | 山东狮克现代农业投资有限公司 | A kind of high-activity microorganism bacterial manure suitable for fertigation |
| CN108484278A (en) * | 2018-05-28 | 2018-09-04 | 新疆农业科学院微生物应用研究所（中国新疆-亚美尼亚生物工程研究开发中心） | A kind of high activity bacillus subtilis compound amino acid liquid fertilizer being exclusively used in trickle irrigation |
| CN108531425A (en) * | 2018-04-11 | 2018-09-14 | 山东省花生研究所 | Pseudomonas putida and its application in preventing peanut root disease |
| CN109206250A (en) * | 2018-11-13 | 2019-01-15 | 广东新维生物科技股份有限公司 | A kind of fertilizer solving the problems, such as soil root and preparation method |
| CN109627066A (en) * | 2018-12-26 | 2019-04-16 | 广西遍天原种猪有限责任公司 | Utilize the method for sick dead pig harmless treat production compost |
| CN111676177A (en) * | 2020-07-23 | 2020-09-18 | 中国科学院成都生物研究所 | Germination growth-promoting bacterium composition and application thereof |
| CN114292142A (en) * | 2022-01-24 | 2022-04-08 | 安徽朴茂农业科技有限公司 | Polyuronic acid ecological fertilizer additive and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101698627A (en) * | 2009-11-25 | 2010-04-28 | 北京理工大学 | Multifunctional microbial fertilizer and preparation method thereof |
| CN102399839A (en) * | 2011-05-13 | 2012-04-04 | 中国海洋大学生物工程开发有限公司 | Production process of bacillus psychrosaccharolyticus extracellular polysaccharide and application thereof |
| CN102899264A (en) * | 2012-06-25 | 2013-01-30 | 石河子大学 | Microbial fertilizer and its preparation method and use |
-
2013
- 2013-05-02 CN CN2013101579591A patent/CN103435395A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101698627A (en) * | 2009-11-25 | 2010-04-28 | 北京理工大学 | Multifunctional microbial fertilizer and preparation method thereof |
| CN102399839A (en) * | 2011-05-13 | 2012-04-04 | 中国海洋大学生物工程开发有限公司 | Production process of bacillus psychrosaccharolyticus extracellular polysaccharide and application thereof |
| CN102899264A (en) * | 2012-06-25 | 2013-01-30 | 石河子大学 | Microbial fertilizer and its preparation method and use |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104262057A (en) * | 2014-09-29 | 2015-01-07 | 石河子大学 | Rhizosphere acid fertilizer and preparation method thereof |
| CN104817363A (en) * | 2014-10-09 | 2015-08-05 | 北京绿可欣生物科技有限公司 | Humic acid water-soluble fertilizer, and preparation and application methods thereof |
| CN104671997A (en) * | 2015-01-30 | 2015-06-03 | 姜明光 | Biological liquid compound fertilizer containing monosilicic acid, N, P and K |
| CN105001017A (en) * | 2015-06-16 | 2015-10-28 | 西南科技大学 | Preparation method for desert soil micro drip irrigation microbial bacterial manure |
| CN106748542A (en) * | 2017-02-13 | 2017-05-31 | 山东狮克现代农业投资有限公司 | A kind of high-activity microorganism bacterial manure suitable for fertigation |
| CN108531425A (en) * | 2018-04-11 | 2018-09-14 | 山东省花生研究所 | Pseudomonas putida and its application in preventing peanut root disease |
| CN108531425B (en) * | 2018-04-11 | 2021-04-06 | 山东省花生研究所 | Pseudomonas putida and application thereof in prevention and treatment of peanut root diseases |
| CN108484278A (en) * | 2018-05-28 | 2018-09-04 | 新疆农业科学院微生物应用研究所（中国新疆-亚美尼亚生物工程研究开发中心） | A kind of high activity bacillus subtilis compound amino acid liquid fertilizer being exclusively used in trickle irrigation |
| CN109206250A (en) * | 2018-11-13 | 2019-01-15 | 广东新维生物科技股份有限公司 | A kind of fertilizer solving the problems, such as soil root and preparation method |
| CN109627066A (en) * | 2018-12-26 | 2019-04-16 | 广西遍天原种猪有限责任公司 | Utilize the method for sick dead pig harmless treat production compost |
| CN111676177A (en) * | 2020-07-23 | 2020-09-18 | 中国科学院成都生物研究所 | Germination growth-promoting bacterium composition and application thereof |
| CN111676177B (en) * | 2020-07-23 | 2022-03-18 | 中国科学院成都生物研究所 | Germination growth-promoting bacterium composition and application thereof |
| CN114292142A (en) * | 2022-01-24 | 2022-04-08 | 安徽朴茂农业科技有限公司 | Polyuronic acid ecological fertilizer additive and application thereof |
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Application publication date: 20131211 |