CN103399390B - Biochip scanner phosphor collection object lens - Google Patents
Biochip scanner phosphor collection object lens Download PDFInfo
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- CN103399390B CN103399390B CN201310173583.3A CN201310173583A CN103399390B CN 103399390 B CN103399390 B CN 103399390B CN 201310173583 A CN201310173583 A CN 201310173583A CN 103399390 B CN103399390 B CN 103399390B
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Abstract
The present invention relates to a kind of fluoroscopic examination optical imagery objective lens, particularly be applied in the biochip scanner phosphor collection object lens based on CCD or cmos imaging method, comprise 8 eyeglasses, CCD imaging surface is played from object space, fluorescence signal passes through successively: J1 plano-convex positive lens, J2 double-concave negative lens, the concavo-convex positive lens of J3, J4 plano-convex positive lens, J5 biconvex positive lens, the concavo-convex negative lens of J6, the average lens of J7, J8 convex-concave positive lens, and each lens arrangement is on an optical axis; Wherein J5 biconvex positive lens, J6 concavo-convex negative lens composition cemented doublet, the surface of the close object space of J4 plano-convex positive lens arranges aperture diaphragm, and the average lens of J7 are emitting fluorescence optical filter.Biochip scanner phosphor collection object lens provided by the invention, structure is simple, and cost is low, and visual field is large, can improve sweep velocity fast, significantly improve phosphor collection efficiency and detection sensitivity, go for all visible light wave ranges.
Description
Technical field
The present invention relates to a kind of fluoroscopic examination optical imagery objective lens, be particularly applied in the biochip scanner phosphor collection object lens based on CCD or cmos imaging method.
Background technology
Current most biochip scanner adopts fluorochrome label, utilize strong exciting light irradiating biological chip fluorescence excitation signal, and utilize detector to detect fluorescence intensity, to obtain biochip information, then software process obtains valuable biological information by analysis.
For the fluorescent scanning system using CCD image-forming principle to detect, the quality of quality to checkout equipment of its phosphor collection object lens performance serves key effect, the several important index of its imaging lens: the numerical aperture of (1) camera lens, use the object lens of large-numerical aperture to significantly improve fluorescence detection efficiency, strengthen low level light signal; (2) operating distance; (3) visual field of optical system, visual field is as far as possible larger; (4) picture element is superior, and optical system must ensure the image quality in apparent field; (5) the use wavelength band of optical system; (6) rear cut-off distance of system.
The open CN1851520A of Chinese patent disclosed in 25 days October in 2006, name is called " imaging lens for rapid biochip detects ", disclose a kind of phosphor collection imaging lens of Biochiop quick inspector, although meet quick, efficient, highly sensitive, the advantage such as long reach, Large visual angle of biochip test aspect.But also there is following shortcoming in this camera lens: the length of (1) this camera lens is oversize; (2) imaging of each visual field is not very even; (3) rear cut-off distance of camera lens is little; (4) range of choice of fluorescent reagent is little.
The and for example open CN1657913A of Chinese patent disclosed in 24 days Augusts in 2005, name is called " microarray chip detection system ", and disclosed microarray chip detection system adopts the method for CCD imaging, and this system uses 12 lens, complex structure.
Summary of the invention:
For above-mentioned technical matters, the phosphor collection object lens that the present invention will provide a kind of structure simple, highly sensitive, concrete technical scheme is:
Biochip scanner phosphor collection object lens, comprise 8 eyeglasses, CCD imaging surface is played from object space, fluorescence signal passes through successively: J1 plano-convex positive lens, J2 double-concave negative lens, the concavo-convex positive lens of J3, J4 plano-convex positive lens, J5 biconvex positive lens, the concavo-convex negative lens of J6, the average lens of J7, J8 convex-concave positive lens, and each lens arrangement is on an optical axis; Wherein J5 biconvex positive lens, J6 concavo-convex negative lens composition cemented doublet, the surface of the close object space of J4 plano-convex positive lens arranges aperture diaphragm, and the average lens of J7 are emitting fluorescence optical filter.
J1 plano-convex positive lens, J2 double-concave negative lens, the concavo-convex negative lens of J6, J8 convex-concave positive lens all adopt H-ZF52 type optical glass, and the concavo-convex positive lens of J3, J4 plano-convex positive lens all adopt H-ZBAF3 type optical glass; J5 biconvex positive lens adopts H-ZK6 type optical glass, and the average lens of J7 adopt H-K9 type optical glass.
Eyeglass is all standard spherical lens, processes respectively.
The numerical aperture 0.4 of biochip scanner phosphor collection object lens, the long 5-7mm of operating distance.
The back focal length of biochip scanner phosphor collection object lens from 19mm, to be connected with optical imaging sensor CCD or CMOS.
Biochip scanner phosphor collection object lens provided by the invention, structure is simple, and cost is low, and visual field is large, can improve sweep velocity fast, and camera lens numerical aperture is large, can significantly improve phosphor collection efficiency, improves the detection sensitivity of instrument; Fit in picture CCD/CMOS, clear, that resolution is high fluorescence picture can be obtained; Operating distance is long, facilitates the adjustment between object space and camera lens, guarantees excellent image quality; Camera lens goes for all visible light wave ranges, is very easy to the unrestricted choice of user to fluorescent marker dyes.
Accompanying drawing illustrates:
Fig. 1: lens construction schematic diagram of the present invention;
Fig. 2: the spatial frequency curve map of camera lens of the present invention;
Fig. 3: the disc of confusion schematic diagram of camera lens wide visual field scope of the present invention in picture plane;
Fig. 4: the curvature of field of the present invention and distortion curve figure;
Fig. 5: the optical path difference curve map of camera lens of the present invention;
Fig. 6: the FFT diffraction energy distribution plan of the different view field imagings of camera lens of the present invention.
Embodiment:
Now in conjunction with Figure of description, fluoroscopic examination object lens are described in further detail.
As shown in Figure 1, phosphor collection camera lens of the present invention includes from the object side to the image side successively along optical axis: biochip scanner phosphor collection object lens, comprise 8 eyeglasses, CCD imaging surface is played from object space, fluorescence signal passes through successively: J1 plano-convex positive lens 1, J2 double-concave negative lens 2, the concavo-convex positive lens 3 of J3, J4 plano-convex positive lens 4, J5 biconvex positive lens 5, the concavo-convex negative lens 6 of J6, the average lens 7 of J7, J8 convex-concave positive lens 8, each lens arrangement is on an optical axis, wherein J5 biconvex positive lens 5, the concavo-convex negative lens 6 of J6 forms cemented doublet, the surface of the close object space of J4 plano-convex positive lens 4 arranges aperture diaphragm, the average lens 7 of J7 are emitting fluorescence optical filter.
The concavo-convex negative lens 6 of J1 plano-convex positive lens 1, J2 double-concave negative lens 2, J6, J8 convex-concave positive lens 8 all adopt H-ZF52 type optical glass, and the concavo-convex positive lens 3 of J3, J4 plano-convex positive lens 4 all adopt H-ZBAF3 type optical glass; J5 biconvex positive lens 5 adopts H-ZK6 type optical glass, and the average lens 7 of J7 adopt H-K9 type optical glass.
Each mirror design parameter of the eyeglass of biochip scanner phosphor collection object lens is as shown in the table successively, (unit: millimeter) range of size error ± 0.2mm:
Fluorescent dye on biochip is generation fluorescence signal under the exciting of incident light, and part fluorescence enters imaging lens, then arrives last detection image planes by above-mentioned a series of lens successively, and CCD camera is placed in test side.Because be collect the such feeble signal of emitting fluorescence, so the preferential CCD camera used with refrigerating function, the ground unrest of signal can be reduced like this, improve signal to noise ratio (S/N ratio).
Average lens 7 are emitting fluorescence optical filter, according to using different fluorescent dyes, can switch to different fluorescence interference optical filters.This camera lens can carry out the rectification of aberration for all visible light wave ranges, be convenient to select different fluorescent dye.
Fig. 2 is spatial frequency curve map of the present invention, and in figure, horizontal ordinate is spatial frequency, and ordinate is optical transfer function.Optical transfer function method evaluates the main method of optical system imaging quality.When frequency 80lp/mm, the optical transfer function of whole visual field, still more than 0.25, meets imaging requirements.
Fig. 3 be full filed scope as the disc of confusion figure in plane, phosphor collection object lens in whole visual field, the various aberration of visible ray full band range inner equilibrium.
Fig. 4 is the curvature of field of the present invention and distortion curve figure, and in figure, left-half is curvature of field curve map, and right half part is distortion curve figure.
Fig. 5 is optical path difference curve map of the present invention, can find out, the aberration balancing of each visual field is more homogeneous.
Fig. 6 is the transfer efficiency of diffraction energy of the present invention, and as can be seen from the figure within spot radius 7um, the transfer efficiency of diffraction energy is greater than 50%.
Can find out that this imaging lens shows excellent optical property from these curve maps above.
Claims (6)
1. biochip scanner phosphor collection object lens, be made up of 8 eyeglasses, it is characterized in that: play CCD imaging surface from object space, fluorescence signal passes through successively: J1 plano-convex positive lens (1), J2 double-concave negative lens (2), the concavo-convex positive lens of J3 (3), J4 plano-convex positive lens (4), J5 biconvex positive lens (5), the concavo-convex negative lens of J6 (6), the average lens of J7 (7), J8 convex-concave positive lens (8), and each lens arrangement is on an optical axis; Wherein J5 biconvex positive lens (5), the concavo-convex negative lens of J6 (6) composition cemented doublet, the surface of the close object space of J4 plano-convex positive lens (4) arranges aperture diaphragm, and the average lens of J7 (7) are emitting fluorescence optical filter.
2. biochip scanner phosphor collection object lens according to claim 1, it is characterized in that: described J1 plano-convex positive lens (1), J2 double-concave negative lens (2), the concavo-convex negative lens of J6 (6), J8 convex-concave positive lens (8) all adopt H-ZF52 type optical glass, the concavo-convex positive lens of J3 (3), J4 plano-convex positive lens (4) all adopt H-ZBAF3 type optical glass; J5 biconvex positive lens (5) adopts H-ZK6 type optical glass, and the average lens of J7 (7) adopt H-K9 type optical glass.
3. biochip scanner phosphor collection object lens according to claim 1 and 2, is characterized in that: the design parameter of described each minute surface of eyeglass is as follows successively,
Unit is millimeter, range of size error ± 0.2mm.
4. biochip scanner phosphor collection object lens according to claim 1 and 2, is characterized in that: eyeglass is all standard spherical lens.
5. biochip scanner phosphor collection object lens according to claim 1 and 2, is characterized in that: the numerical aperture 0.4 of biochip scanner phosphor collection object lens, the long 5-7mm of operating distance.
6. biochip scanner phosphor collection object lens according to claim 1 and 2, is characterized in that: the back focal length of biochip scanner phosphor collection object lens is from 19mm.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310173583.3A CN103399390B (en) | 2013-05-10 | 2013-05-10 | Biochip scanner phosphor collection object lens |
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| CN201310173583.3A CN103399390B (en) | 2013-05-10 | 2013-05-10 | Biochip scanner phosphor collection object lens |
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| CN103399390A CN103399390A (en) | 2013-11-20 |
| CN103399390B true CN103399390B (en) | 2015-12-02 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112505879B (en) * | 2020-11-18 | 2023-11-24 | 西安玄瑞光电科技有限公司 | Wide-view-field long-focus high-resolution lens |
| CN113866966B (en) * | 2021-10-11 | 2023-05-02 | 深圳赛陆医疗科技有限公司 | Imaging lens of gene sequencer, gene sequencer and gene sequencing system |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5052763A (en) * | 1990-08-28 | 1991-10-01 | International Business Machines Corporation | Optical system with two subsystems separately correcting odd aberrations and together correcting even aberrations |
| EP0863440A2 (en) * | 1997-02-28 | 1998-09-09 | Canon Kabushiki Kaisha | Projection exposure apparatus and device manufacturing method |
| CN1851520A (en) * | 2006-05-30 | 2006-10-25 | 南开大学 | Imaging lens for rapid biochip detection |
| US7382552B2 (en) * | 2004-09-08 | 2008-06-03 | Canon Kabushiki Kaisha | Zoom optical system |
| CN203232193U (en) * | 2013-05-10 | 2013-10-09 | 无锡国盛生物工程有限公司 | Fluorescence collection object lens of biological chip scanner |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004020819A (en) * | 2002-06-14 | 2004-01-22 | Pentax Corp | Zoom lens system |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5052763A (en) * | 1990-08-28 | 1991-10-01 | International Business Machines Corporation | Optical system with two subsystems separately correcting odd aberrations and together correcting even aberrations |
| EP0863440A2 (en) * | 1997-02-28 | 1998-09-09 | Canon Kabushiki Kaisha | Projection exposure apparatus and device manufacturing method |
| US7382552B2 (en) * | 2004-09-08 | 2008-06-03 | Canon Kabushiki Kaisha | Zoom optical system |
| CN1851520A (en) * | 2006-05-30 | 2006-10-25 | 南开大学 | Imaging lens for rapid biochip detection |
| CN203232193U (en) * | 2013-05-10 | 2013-10-09 | 无锡国盛生物工程有限公司 | Fluorescence collection object lens of biological chip scanner |
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