CN103399390A - Fluorescence collecting object lens of biological chip scanner - Google Patents
Fluorescence collecting object lens of biological chip scanner Download PDFInfo
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- CN103399390A CN103399390A CN2013101735833A CN201310173583A CN103399390A CN 103399390 A CN103399390 A CN 103399390A CN 2013101735833 A CN2013101735833 A CN 2013101735833A CN 201310173583 A CN201310173583 A CN 201310173583A CN 103399390 A CN103399390 A CN 103399390A
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Abstract
The invention relates to a fluorescence collecting object lens of a biological chip scanner, which is based on a CCD or CMOS imaging method. The fluorescence collecting object lens comprises eight lenses, and fluorescence signals successively penetrate through a plano-convex positive lens J1, a double-concave negative lens J2, a concave-convex positive lens J3, a plano-concave positive lens J4, a plano-convex positive lens J5, a concave-convex negative lens J6, a plano-plane lens J7 and a convex-concave positive lens J8 from an object side to a CCD imaging surface, wherein the lenses are arranged on a optical axis, the double-convex positive lens J5 and the concave-convex negative lens J6 form a double gummed lens, an aperture diaphragm is arranged on the surface of the plano-convex positive lens J4 close to the object side, and the plano-plane lens J7 is an optical filter of emission fluorescence. The fluorescence collecting object lens of the biological chip scanner provided by the invention is simple in structure, low in cost, and large in view field, can rapidly increase scanning speed, substantially improves the fluorescence collection efficiency and detection flexibility, and can be suitable for all visible optical bands.
Description
Technical field
The present invention relates to a kind of fluoroscopic examination optical imagery objective lens, particularly be applied in the biochip scanner phosphor collection object lens based on CCD or cmos imaging method.
Background technology
Present most biochip scanner adopts fluorochrome label, utilize strong exciting light irradiating biological chip fluorescence excitation signal, and utilize detector to survey fluorescence intensity, to obtain biochip information, then software is processed and is obtained valuable biological information by analysis.
For the fluorescent scanning system of using the CCD image-forming principle to detect, the quality of its phosphor collection object lens performance has played key effect to the quality of checkout equipment, the several important index of its imaging lens: the numerical aperture of (1) camera lens, use the object lens of large-numerical aperture can significantly improve fluoroscopic examination efficiency, strengthen low level light signal; (2) operating distance; (3) visual field of optical system, visual field is as far as possible larger; (4) picture element is superior, and optical system must guarantee the image quality in apparent field; (5) the use wavelength band of optical system; (6) rear cut-off distance of system.
The open CN1851520A of disclosed Chinese patent on October 25th, 2006, name is called " for the imaging lens of rapid biochip detection ", a kind of phosphor collection imaging lens of Biochiop quick inspector is disclosed, although met the advantages such as quick, efficient, highly sensitive, the long reach of biochip test aspect, large visual field.But also there is following shortcoming in this camera lens: the length of (1) this camera lens is oversize; (2) imaging of each visual field is not very even; (3) rear cut-off distance of camera lens is little; (4) range of choice of fluorescent reagent is little.
And for example on August 24th, 2005, disclosed Chinese patent disclosed CN1657913A, and name is called " microarray chip detection system ", and disclosed microarray chip detection system adopts the method for CCD imaging, and this system is used 12 lens, complex structure.
Summary of the invention:
For above-mentioned technical matters, the present invention will provide a kind of simple in structure, highly sensitive phosphor collection object lens, and concrete technical scheme is:
Biochip scanner phosphor collection object lens, comprise 8 eyeglasses, from object space, play the CCD imaging surface, fluorescence signal passes through successively: J1 plano-convex positive lens, J2 double-concave negative lens, the concavo-convex positive lens of J3, J4 plano-convex positive lens, J5 biconvex positive lens, the concavo-convex negative lens of J6, the average lens of J7, J8 convex-concave positive lens, and each lens arrangement is on an optical axis; Wherein the concavo-convex negative lens of J5 biconvex positive lens, J6 forms cemented doublet, on the surface of the close object space of J4 plano-convex positive lens, aperture diaphragm is set, and the average lens of J7 are the emitting fluorescence optical filter.
J1 plano-convex positive lens, J2 double-concave negative lens, the concavo-convex negative lens of J6, J8 convex-concave positive lens all adopt H-ZF52 type optical glass, and the concavo-convex positive lens of J3, J4 plano-convex positive lens all adopt H-ZBAF3 type optical glass; J5 biconvex positive lens adopts H-ZK6 type optical glass, and the average lens of J7 adopt H-K9 type optical glass.
Eyeglass is all standard spherical lens, respectively processing.
The numerical aperture 0.4 of camera lens, the long 5-7mm of operating distance.
The back focal length of camera lens is from 19mm, in order to be connected with optical imaging sensor CCD or CMOS.
The total length of whole camera lens is no more than 80mm.
Biochip scanner phosphor collection object lens provided by the invention, simple in structure, cost is low, and visual field is large, can improve fast sweep velocity, and the camera lens numerical aperture is large, can significantly improve phosphor collection efficiency, improves the detection sensitivity of instrument; Fit in picture CCD/CMOS, can obtain the fluorescence picture clear, that resolution is high; Operating distance is long, facilitates the adjusting between object space and camera lens, guarantees good image quality; Camera lens goes for all visible light wave ranges, is very easy to user's freely selecting fluorescent marker dyes.
The accompanying drawing explanation:
Fig. 1: lens construction schematic diagram of the present invention;
Fig. 2: the spatial frequency curve map of camera lens of the present invention;
Fig. 3: the disc of confusion schematic diagram of the wide field range of camera lens of the present invention on the picture plane;
Fig. 4: the curvature of field of the present invention and distortion curve figure;
Fig. 5: the optical path difference curve map of camera lens of the present invention;
Fig. 6: the FFT diffraction energy distribution plan of the different view field imagings of camera lens of the present invention.
Embodiment:
Now in conjunction with Figure of description, the fluoroscopic examination object lens are described in further detail.
as shown in Figure 1, phosphor collection camera lens of the present invention includes from the object side to the image side successively along optical axis: biochip scanner phosphor collection object lens, comprise 8 eyeglasses, from object space, play the CCD imaging surface, fluorescence signal passes through successively: J1 plano-convex positive lens 1, J2 double-concave negative lens 2, the concavo-convex positive lens 3 of J3, J4 plano-convex positive lens 4, J5 biconvex positive lens 5, the concavo-convex negative lens 6 of J6, the average lens 7 of J7, J8 convex-concave positive lens 8, each lens arrangement is on an optical axis, J5 biconvex positive lens 5 wherein, the concavo-convex negative lens 6 of J6 forms cemented doublet, on the surface of the close object space of J4 plano-convex positive lens 4, aperture diaphragm is set, the average lens 7 of J7 are the emitting fluorescence optical filter.
J1 plano-convex positive lens 1, J2 double-concave negative lens 2, the concavo-convex negative lens 6 of J6, J8 convex-concave positive lens 8 all adopt H-ZF52 type optical glass, and the concavo-convex positive lens 3 of J3, J4 plano-convex positive lens 4 all adopt H-ZBAF3 type optical glass; J5 biconvex positive lens 5 adopts H-ZK6 type optical glass, and the average lens 7 of J7 adopt H-K9 type optical glass.
It is as shown in the table successively that the minute surface Basic Design of camera lens is counted parameter, (unit: millimeter) range of size error ± 0.2mm:
Fluorescent dye on biochip is the generation fluorescence signal under the exciting of incident light, and part fluorescence enters imaging lens, then by above-mentioned a series of lens, arrives last detection image planes successively, and the CCD camera is placed in test side.Because be to collect the such feeble signal of emitting fluorescence, so preferential use, with the CCD camera of refrigerating function, can reduce the ground unrest of signal like this, improve signal to noise ratio (S/N ratio).
Average lens 7 are the emitting fluorescence optical filter, according to using different fluorescent dyes, can switch to different fluorescence interference filters.This camera lens can carry out the rectification of aberration for all visible light wave ranges, be convenient to select different fluorescent dyes.
Fig. 2 is spatial frequency curve map of the present invention, and in figure, horizontal ordinate is spatial frequency, and ordinate is optical transfer function.The optical transfer function method is to estimate the main method of optical system imaging quality.When frequency 80lp/mm, the optical transfer function of whole visual field still, more than 0.25, has met imaging requirements.
Fig. 3 be full field range as the disc of confusion figure on plane, the phosphor collection object lens in whole visual field, the various aberrations of visible light all band scope inner equilibrium.
Fig. 4 is the curvature of field of the present invention and distortion curve figure, and in figure, left-half is curvature of field curve map, and right half part is distortion curve figure.
Fig. 5 is optical path difference curve map of the present invention, can find out, the aberration balancing of each visual field is homogeneous relatively.
Fig. 6 is the transfer efficiency of diffraction energy of the present invention, and as can be seen from the figure in spot radius 7um, the transfer efficiency of diffraction energy is greater than 50%.
From above these curve maps, finding out the good optical property of this imaging lens performance.
Claims (7)
1. biochip scanner phosphor collection object lens, comprise 8 eyeglasses, it is characterized in that: from object space, play the CCD imaging surface, fluorescence signal passes through successively: J1 plano-convex positive lens (1), J2 double-concave negative lens (2), the concavo-convex positive lens of J3 (3), J4 plano-convex positive lens (4), J5 biconvex positive lens (5), the concavo-convex negative lens of J6 (6), the average lens of J7 (7), J8 convex-concave positive lens (8), and each lens arrangement is on an optical axis; Wherein J5 biconvex positive lens (5), the concavo-convex negative lens of J6 (6) form cemented doublet, on the surface of the close object space of J4 plano-convex positive lens (4), aperture diaphragm are set, and the average lens of J7 (7) are the emitting fluorescence optical filter.
2. biochip scanner phosphor collection object lens according to claim 1, it is characterized in that: described J1 plano-convex positive lens (1), J2 double-concave negative lens (2), the concavo-convex negative lens of J6 (6), J8 convex-concave positive lens (8) all adopt H-ZF52 type optical glass, and the concavo-convex positive lens of J3 (3), J4 plano-convex positive lens (4) all adopt H-ZBAF3 type optical glass; J5 biconvex positive lens (5) adopts H-ZK6 type optical glass, and the average lens of J7 (7) adopt H-K9 type optical glass.
3. biochip scanner phosphor collection object lens according to claim 1 and 2, it is characterized in that: it is as follows successively that the Basic Design of described each minute surface of camera lens is counted parameter, (unit: millimeter) range of size error ± 0.2mm:
Camera lens surface sequence number radius-of-curvature vertex distance D optical index abbe number
1 Infinity 2.84 1.84663 23.784819
2 -11.22 2.3
3 -6.127 8.8 1.84663 23.784819
4 83.37 1.97
5 -84 7 1.656913 51.12808
6 -17.498 0.6
7 Infinity 6.79 1.656913 51.12808
8 -21.28 8.6
9 42.09 10 1.612718 58.579159
10 -22.59 2.7 1.84663 23.784819
11 -101.16 17
12 Infinity 2 1.516371 64.133559
13 Infinity 8.5
14 38.25 2.45 1.84663 23.784819
15 67.925 19。
4. biochip scanner phosphor collection object lens according to claim 1 and 2, it is characterized in that: eyeglass is all standard spherical lens.
5. biochip scanner phosphor collection object lens according to claim 1 and 2, is characterized in that: the numerical aperture 0.4 of camera lens, the long 5-7mm of operating distance.
6. biochip scanner phosphor collection object lens according to claim 1 and 2, it is characterized in that: the back focal length of camera lens is from 19mm.
7. biochip scanner phosphor collection object lens according to claim 1 and 2, it is characterized in that: the total length of whole camera lens is no more than 80mm.
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| CN201310173583.3A CN103399390B (en) | 2013-05-10 | 2013-05-10 | Biochip scanner phosphor collection object lens |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112505879A (en) * | 2020-11-18 | 2021-03-16 | 西安玄瑞光电科技有限公司 | Wide-field-of-view long-focus high-resolution lens |
| CN113866966A (en) * | 2021-10-11 | 2021-12-31 | 深圳赛陆医疗科技有限公司 | Imaging lens of gene sequencer, gene sequencer and gene sequencing system |
Citations (6)
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|---|---|---|---|---|
| US5052763A (en) * | 1990-08-28 | 1991-10-01 | International Business Machines Corporation | Optical system with two subsystems separately correcting odd aberrations and together correcting even aberrations |
| EP0863440A2 (en) * | 1997-02-28 | 1998-09-09 | Canon Kabushiki Kaisha | Projection exposure apparatus and device manufacturing method |
| JP2004020819A (en) * | 2002-06-14 | 2004-01-22 | Pentax Corp | Zoom lens system |
| CN1851520A (en) * | 2006-05-30 | 2006-10-25 | 南开大学 | Imaging lens for rapid biochip detection |
| US7382552B2 (en) * | 2004-09-08 | 2008-06-03 | Canon Kabushiki Kaisha | Zoom optical system |
| CN203232193U (en) * | 2013-05-10 | 2013-10-09 | 无锡国盛生物工程有限公司 | Fluorescence collection object lens of biological chip scanner |
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2013
- 2013-05-10 CN CN201310173583.3A patent/CN103399390B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5052763A (en) * | 1990-08-28 | 1991-10-01 | International Business Machines Corporation | Optical system with two subsystems separately correcting odd aberrations and together correcting even aberrations |
| EP0863440A2 (en) * | 1997-02-28 | 1998-09-09 | Canon Kabushiki Kaisha | Projection exposure apparatus and device manufacturing method |
| EP0863440B1 (en) * | 1997-02-28 | 2003-04-23 | Canon Kabushiki Kaisha | Projection exposure apparatus and device manufacturing method |
| JP2004020819A (en) * | 2002-06-14 | 2004-01-22 | Pentax Corp | Zoom lens system |
| US7382552B2 (en) * | 2004-09-08 | 2008-06-03 | Canon Kabushiki Kaisha | Zoom optical system |
| CN1851520A (en) * | 2006-05-30 | 2006-10-25 | 南开大学 | Imaging lens for rapid biochip detection |
| CN203232193U (en) * | 2013-05-10 | 2013-10-09 | 无锡国盛生物工程有限公司 | Fluorescence collection object lens of biological chip scanner |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112505879A (en) * | 2020-11-18 | 2021-03-16 | 西安玄瑞光电科技有限公司 | Wide-field-of-view long-focus high-resolution lens |
| CN112505879B (en) * | 2020-11-18 | 2023-11-24 | 西安玄瑞光电科技有限公司 | Wide-view-field long-focus high-resolution lens |
| CN113866966A (en) * | 2021-10-11 | 2021-12-31 | 深圳赛陆医疗科技有限公司 | Imaging lens of gene sequencer, gene sequencer and gene sequencing system |
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