CN103396933B - 通过杂交进行的随机阵列dna分析 - Google Patents
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- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/061—Sources
- G01N2201/06113—Coherent sources; lasers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2223/00—Investigating materials by wave or particle radiation
- G01N2223/40—Imaging
- G01N2223/413—Imaging sensor array [CCD]
Abstract
Description
靶 | 5'-探针的FM/SMM | 3'-探针的FM/SMM |
Tgt1 | 14 | 20 |
Tgt2 | 7 | 12 |
Tgt3 | 9 | 16 |
Tgt4 | 4 | 4 |
Claims (10)
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US60/450,566 | 2003-02-26 | ||
CN2004800108063A CN1791682B (zh) | 2003-02-26 | 2004-02-26 | 通过杂交进行的随机阵列dna分析 |
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CN103396933B true CN103396933B (zh) | 2016-04-20 |
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Families Citing this family (161)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6401267B1 (en) * | 1993-09-27 | 2002-06-11 | Radoje Drmanac | Methods and compositions for efficient nucleic acid sequencing |
AU2004214891B2 (en) | 2003-02-26 | 2010-01-07 | Complete Genomics, Inc. | Random array DNA analysis by hybridization |
US8114978B2 (en) * | 2003-08-05 | 2012-02-14 | Affymetrix, Inc. | Methods for genotyping selected polymorphism |
US7169560B2 (en) * | 2003-11-12 | 2007-01-30 | Helicos Biosciences Corporation | Short cycle methods for sequencing polynucleotides |
US7276338B2 (en) | 2003-11-17 | 2007-10-02 | Jacobson Joseph M | Nucleotide sequencing via repetitive single molecule hybridization |
EP1841879A4 (en) | 2005-01-25 | 2009-05-27 | Population Genetics Technologi | ISOTHERMAL DNA AMPLIFICATION |
SG162795A1 (en) | 2005-06-15 | 2010-07-29 | Callida Genomics Inc | Single molecule arrays for genetic and chemical analysis |
EP2302070B1 (en) | 2005-06-23 | 2012-08-22 | Keygene N.V. | Strategies for high throughput identification and detection of polymorphisms |
GB0514935D0 (en) | 2005-07-20 | 2005-08-24 | Solexa Ltd | Methods for sequencing a polynucleotide template |
US10316364B2 (en) | 2005-09-29 | 2019-06-11 | Keygene N.V. | Method for identifying the source of an amplicon |
ES2338459T5 (es) | 2005-09-29 | 2014-02-24 | Keygene N.V. | Exploración de alto rendimiento de poblaciones mutagenizadas |
WO2007044245A2 (en) * | 2005-10-07 | 2007-04-19 | Callida Genomics, Inc. | Self-assembled single molecule arrays and uses thereof |
EP2363504A1 (en) | 2005-12-22 | 2011-09-07 | Keygene N.V. | Method for high-throughtput AFLP-based polymorphism detection |
WO2007087262A2 (en) | 2006-01-23 | 2007-08-02 | Population Genetics Technologies Ltd. | Selective genome amplification |
CN100510105C (zh) * | 2006-02-08 | 2009-07-08 | 博奥生物有限公司 | 一种序列特异性寡核苷酸探针及其应用 |
EP1987159B2 (en) | 2006-02-08 | 2020-08-12 | Illumina Cambridge Limited | Method for sequencing a polynucleotide template |
SG10201405158QA (en) | 2006-02-24 | 2014-10-30 | Callida Genomics Inc | High throughput genome sequencing on dna arrays |
CN101432439B (zh) * | 2006-02-24 | 2013-07-24 | 考利达基因组股份有限公司 | Dna阵列上的高通量基因组测序 |
WO2007123744A2 (en) * | 2006-03-31 | 2007-11-01 | Solexa, Inc. | Systems and devices for sequence by synthesis analysis |
US20090253581A1 (en) | 2006-04-04 | 2009-10-08 | Keygene N.V. | High Throughput Detection of Molecular Markers Based on AFLP and High Throughput Sequencing |
US7923054B2 (en) * | 2006-04-19 | 2011-04-12 | Gore Enterprise Holdings, Inc. | Functional porous substrates for attaching biomolecules |
EP2027442A2 (en) * | 2006-05-16 | 2009-02-25 | Applied Biosystems, Inc. | Systems, methods, and apparatus for single molecule sequencing |
JP5081232B2 (ja) * | 2006-05-22 | 2012-11-28 | ナノストリング テクノロジーズ, インコーポレイテッド | ナノレポーターを分析するためのシステムおよび方法 |
JP4947573B2 (ja) * | 2006-08-02 | 2012-06-06 | 独立行政法人科学技術振興機構 | マイクロアレイデータの解析方法及び解析装置 |
US7947487B2 (en) | 2006-10-05 | 2011-05-24 | Massachusetts Institute Of Technology | Multifunctional encoded particles for high-throughput analysis |
US7910354B2 (en) * | 2006-10-27 | 2011-03-22 | Complete Genomics, Inc. | Efficient arrays of amplified polynucleotides |
US20080221832A1 (en) * | 2006-11-09 | 2008-09-11 | Complete Genomics, Inc. | Methods for computing positional base probabilities using experminentals base value distributions |
US20090111705A1 (en) * | 2006-11-09 | 2009-04-30 | Complete Genomics, Inc. | Selection of dna adaptor orientation by hybrid capture |
CN101275168B (zh) * | 2007-03-13 | 2013-06-05 | 拜奥希格马公司 | 核苷酸序列阵列和使用该阵列的方法 |
JP5020734B2 (ja) * | 2007-07-31 | 2012-09-05 | 株式会社日立ハイテクノロジーズ | 核酸解析方法及び装置 |
JP2009036687A (ja) * | 2007-08-03 | 2009-02-19 | Panasonic Corp | 遺伝子検出方法 |
WO2009052214A2 (en) * | 2007-10-15 | 2009-04-23 | Complete Genomics, Inc. | Sequence analysis using decorated nucleic acids |
US7897344B2 (en) * | 2007-11-06 | 2011-03-01 | Complete Genomics, Inc. | Methods and oligonucleotide designs for insertion of multiple adaptors into library constructs |
US8518640B2 (en) * | 2007-10-29 | 2013-08-27 | Complete Genomics, Inc. | Nucleic acid sequencing and process |
US8415099B2 (en) | 2007-11-05 | 2013-04-09 | Complete Genomics, Inc. | Efficient base determination in sequencing reactions |
US9382585B2 (en) | 2007-10-30 | 2016-07-05 | Complete Genomics, Inc. | Apparatus for high throughput sequencing of nucleic acids |
WO2009061840A1 (en) * | 2007-11-05 | 2009-05-14 | Complete Genomics, Inc. | Methods and oligonucleotide designs for insertion of multiple adaptors employing selective methylation |
WO2009073629A2 (en) | 2007-11-29 | 2009-06-11 | Complete Genomics, Inc. | Efficient shotgun sequencing methods |
US8592150B2 (en) | 2007-12-05 | 2013-11-26 | Complete Genomics, Inc. | Methods and compositions for long fragment read sequencing |
EP4053546A1 (en) * | 2007-12-06 | 2022-09-07 | Genalyte, Inc. | Device and method for performing label-free monitoring of processes. |
EP2245462B1 (en) * | 2008-01-22 | 2017-05-31 | Koninklijke Philips N.V. | Detection of target components with the help of indicator particles |
WO2009094583A1 (en) * | 2008-01-23 | 2009-07-30 | Complete Genomics, Inc. | Methods and compositions for preventing bias in amplification and sequencing reactions |
WO2009097368A2 (en) | 2008-01-28 | 2009-08-06 | Complete Genomics, Inc. | Methods and compositions for efficient base calling in sequencing reactions |
WO2009140339A1 (en) * | 2008-05-14 | 2009-11-19 | Opgen, Inc. | Methods of determining properties of molecules |
ES2437610T3 (es) | 2008-07-17 | 2014-01-13 | Ikfe Institut Für Klinische Forschung Und Entwicklung Gmbh | Biomarcadores para cardiodiabetes |
WO2010009438A1 (en) | 2008-07-17 | 2010-01-21 | Andreas Pfuetzner | Biomarkers for insulin resistance and beta-cell dysfunction |
WO2010062627A2 (en) | 2008-10-27 | 2010-06-03 | Genalyte Inc. | Biosensors based on optical probing and sensing |
US20100256196A1 (en) | 2008-12-04 | 2010-10-07 | Ikfe | Biomarkers for Atherosclerosis |
US20110118134A1 (en) | 2008-12-11 | 2011-05-19 | Ikfe Gmbh | Biomarkers for insulin sensitizer drug response |
EP2382467A1 (en) | 2008-12-30 | 2011-11-02 | IKFE GmbH | Biomarkers for adipose tissue activity |
US20100210541A1 (en) | 2009-01-07 | 2010-08-19 | Andreas Pfuetzner | Biomarkers for Appetite Regulation |
AU2010257118B2 (en) * | 2009-06-04 | 2014-08-28 | Lockheed Martin Corporation | Multiple-sample microfluidic chip for DNA analysis |
US9524369B2 (en) | 2009-06-15 | 2016-12-20 | Complete Genomics, Inc. | Processing and analysis of complex nucleic acid sequence data |
WO2011012280A1 (en) * | 2009-07-30 | 2011-02-03 | Roche Diagnostics Gmbh | A set of oligonucleotide probes as well as methods and uses related thereto |
US9023769B2 (en) | 2009-11-30 | 2015-05-05 | Complete Genomics, Inc. | cDNA library for nucleic acid sequencing |
WO2011068518A1 (en) * | 2009-12-04 | 2011-06-09 | Massachusetts Institute Of Technology | Multiplexed quantitative pcr end point analysis of nucleic acid targets |
US20110136104A1 (en) * | 2009-12-04 | 2011-06-09 | Massachusetts Institute Of Technology | Multiplexed quantitative pcr end point analysis of nucleic acid targets |
US20190300945A1 (en) | 2010-04-05 | 2019-10-03 | Prognosys Biosciences, Inc. | Spatially Encoded Biological Assays |
US10787701B2 (en) | 2010-04-05 | 2020-09-29 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
CA2802049C (en) | 2010-06-07 | 2018-07-10 | Firefly Bioworks, Inc. | Scanning multifunctional particles |
US8586301B2 (en) | 2010-06-30 | 2013-11-19 | Stratos Genomics, Inc. | Multiplexed identification of nucleic acid sequences |
CN103228796B (zh) | 2010-08-11 | 2016-06-01 | 赛路拉公司 | 基因分型dna |
JP6114694B2 (ja) | 2010-10-04 | 2017-04-12 | ジナプシス インコーポレイテッド | 自動化された再使用可能な並行生物反応のための系および方法 |
US9184099B2 (en) | 2010-10-04 | 2015-11-10 | The Board Of Trustees Of The Leland Stanford Junior University | Biosensor devices, systems and methods therefor |
US8725422B2 (en) | 2010-10-13 | 2014-05-13 | Complete Genomics, Inc. | Methods for estimating genome-wide copy number variations |
GB2497501A (en) | 2010-10-15 | 2013-06-12 | Lockheed Corp | Micro fluidic optic design |
WO2012061778A2 (en) | 2010-11-05 | 2012-05-10 | Genalyte, Inc. | Optical analyte detection systems and methods of use |
WO2012078321A1 (en) * | 2010-12-10 | 2012-06-14 | The Board Of Trustees Of The Leland Stanford Junior University | Phased genome sequencing |
US10208334B2 (en) * | 2011-04-12 | 2019-02-19 | Michael D. Okura | Systems, methods, and compositions for enhancing the specificity of nucleic acid hybridization |
GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
US9926596B2 (en) | 2011-05-27 | 2018-03-27 | Genapsys, Inc. | Systems and methods for genetic and biological analysis |
US8585973B2 (en) | 2011-05-27 | 2013-11-19 | The Board Of Trustees Of The Leland Stanford Junior University | Nano-sensor array |
US10501791B2 (en) | 2011-10-14 | 2019-12-10 | President And Fellows Of Harvard College | Sequencing by structure assembly |
US10837879B2 (en) | 2011-11-02 | 2020-11-17 | Complete Genomics, Inc. | Treatment for stabilizing nucleic acid arrays |
WO2013067489A1 (en) * | 2011-11-05 | 2013-05-10 | President And Fellows Of Harvard College | Nucleic acid-based linkers for detecting and measuring interactions |
CN104105797B (zh) | 2011-12-01 | 2016-08-31 | 吉纳普赛斯股份有限公司 | 用于高效电子测序与检测的系统和方法 |
US11021737B2 (en) | 2011-12-22 | 2021-06-01 | President And Fellows Of Harvard College | Compositions and methods for analyte detection |
US10227639B2 (en) | 2011-12-22 | 2019-03-12 | President And Fellows Of Harvard College | Compositions and methods for analyte detection |
US9322054B2 (en) | 2012-02-22 | 2016-04-26 | Lockheed Martin Corporation | Microfluidic cartridge |
EP2844771A4 (en) | 2012-05-04 | 2015-12-02 | Complete Genomics Inc | METHOD FOR DETERMINING THE ABSOLUTE GENOME-WIDE COPY COUNTER CHANGES OF COMPLEX TUMORS |
WO2013184754A2 (en) | 2012-06-05 | 2013-12-12 | President And Fellows Of Harvard College | Spatial sequencing of nucleic acids using dna origami probes |
US10347361B2 (en) | 2012-10-24 | 2019-07-09 | Nantomics, Llc | Genome explorer system to process and present nucleotide variations in genome sequence data |
US10138509B2 (en) | 2013-03-12 | 2018-11-27 | President And Fellows Of Harvard College | Method for generating a three-dimensional nucleic acid containing matrix |
WO2014143637A1 (en) | 2013-03-15 | 2014-09-18 | The Board Of Trustees Of The University Of Illinois | Methods and compositions for enhancing immunoassays |
US9328382B2 (en) | 2013-03-15 | 2016-05-03 | Complete Genomics, Inc. | Multiple tagging of individual long DNA fragments |
EP2971141B1 (en) | 2013-03-15 | 2018-11-28 | Genapsys, Inc. | Systems for biological analysis |
WO2014210225A1 (en) | 2013-06-25 | 2014-12-31 | Prognosys Biosciences, Inc. | Methods and systems for determining spatial patterns of biological targets in a sample |
SG11201601201VA (en) * | 2013-08-19 | 2016-03-30 | Singular Bio Inc | Assays for single molecule detection and use thereof |
CN103484561A (zh) * | 2013-10-18 | 2014-01-01 | 南京农业大学 | 一种核酸单分子检测方法 |
US10125393B2 (en) | 2013-12-11 | 2018-11-13 | Genapsys, Inc. | Systems and methods for biological analysis and computation |
EP3132060B1 (en) | 2014-04-18 | 2019-03-13 | Genapsys Inc. | Methods and systems for nucleic acid amplification |
US10179932B2 (en) | 2014-07-11 | 2019-01-15 | President And Fellows Of Harvard College | Methods for high-throughput labelling and detection of biological features in situ using microscopy |
US11198900B2 (en) | 2014-12-06 | 2021-12-14 | Children's Medical Center Corporation | Nucleic acid-based linkers for detecting and measuring interactions |
CN107735497B (zh) * | 2015-02-18 | 2021-08-20 | 卓异生物公司 | 用于单分子检测的测定及其应用 |
US10774374B2 (en) | 2015-04-10 | 2020-09-15 | Spatial Transcriptomics AB and Illumina, Inc. | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
US11396650B2 (en) | 2015-06-02 | 2022-07-26 | Children's Medical Center Corporation | Nucleic acid complexes for screening barcoded compounds |
WO2017027835A1 (en) * | 2015-08-12 | 2017-02-16 | Circulogene Theranostics, Llc | Method of preparing cell free nucleic acid molecules by in situ amplification |
MX2018005611A (es) | 2015-11-03 | 2018-11-09 | Harvard College | Metodo y aparato para la formacion de imagenes volumetricas de una matriz tridimensional que contiene acido nucleico. |
WO2017139409A1 (en) | 2016-02-09 | 2017-08-17 | Children's Medical Center Corporation | Method for detection of analytes via polymer complexes |
CA3018582A1 (en) * | 2016-03-25 | 2017-09-28 | Bioceryx Inc. | Apparatuses and methods for assessing target sequence numbers |
US20170290292A1 (en) * | 2016-04-08 | 2017-10-12 | Empire Technology Development Llc | Coatings for dag prevention |
CN109415761B (zh) | 2016-04-25 | 2022-09-20 | 哈佛学院董事及会员团体 | 用于原位分子检测的杂交链反应方法 |
US11352667B2 (en) | 2016-06-21 | 2022-06-07 | 10X Genomics, Inc. | Nucleic acid sequencing |
EP3488017A4 (en) | 2016-07-20 | 2020-02-26 | Genapsys Inc. | SYSTEMS AND METHODS FOR NUCLEIC ACID SEQUENCING |
CN109983125A (zh) | 2016-08-31 | 2019-07-05 | 哈佛学院董事及会员团体 | 生成用于通过荧光原位测序检测的核酸序列文库的方法 |
CN109923216A (zh) | 2016-08-31 | 2019-06-21 | 哈佛学院董事及会员团体 | 将生物分子的检测组合到使用荧光原位测序的单个试验的方法 |
IL266197B2 (en) | 2016-10-24 | 2024-03-01 | Geneinfosec Inc | Hiding information contained within nucleic acids |
TWI777989B (zh) | 2016-11-03 | 2022-09-21 | 大陸商深圳華大智造科技有限公司 | 用於生物或化學分析的生物感測器以及製造其的方法 |
HUE059673T2 (hu) | 2017-01-04 | 2022-12-28 | Mgi Tech Co Ltd | Nukleinsav-szekvenálás affinitási reagensek felhasználásával |
SG11201908509TA (en) | 2017-03-20 | 2019-10-30 | Mgi Tech Co Ltd | Biosensors for biological or chemical analysis and methods of manufacturing the same |
CN109789167A (zh) | 2017-04-14 | 2019-05-21 | 哈佛学院董事及会员团体 | 用于产生细胞衍生的微丝网络的方法 |
WO2018218150A1 (en) | 2017-05-26 | 2018-11-29 | President And Fellows Of Harvard College | Systems and methods for high-throughput image-based screening |
WO2019060177A1 (en) | 2017-09-19 | 2019-03-28 | Complete Genomics, Inc. | MANUFACTURE OF SEQUENCING FLOW CELLS AT THE WAFER |
MX2020003113A (es) | 2017-09-21 | 2020-09-07 | Genapsys Inc | Sistemas y metodos para secuenciar acido nucleico. |
US11597965B2 (en) | 2017-10-06 | 2023-03-07 | 10X Genomics, Inc. | RNA templated ligation |
US10344328B2 (en) | 2017-11-17 | 2019-07-09 | Ultima Genomics, Inc. | Methods for biological sample processing and analysis |
US11499962B2 (en) | 2017-11-17 | 2022-11-15 | Ultima Genomics, Inc. | Methods and systems for analyte detection and analysis |
US11591636B2 (en) | 2017-11-20 | 2023-02-28 | Children's Medical Center Corporation | Force-controlled nanoswitch assays for single-molecule detection in complex biological fluids |
EP3530755B1 (de) | 2018-02-26 | 2020-08-26 | AGCT GmbH | Verfahren zum anzeigen des fortschrittes der amplifikation von nukleinsäuren und kit zu dessen durchführung |
EP3530756A1 (de) * | 2018-02-26 | 2019-08-28 | AGCT GmbH | Verfahren zur selektiven amplifikation von nukleinsäuren und kit zu dessen durchführung |
CN108642159B (zh) * | 2018-06-27 | 2022-07-01 | 上海宏滩生物科技有限公司 | 生物芯片检测系统 |
EP3836967A4 (en) | 2018-07-30 | 2022-06-15 | ReadCoor, LLC | METHODS AND SYSTEMS FOR SAMPLE PROCESSING OR ANALYSIS |
CN109682784A (zh) * | 2018-12-29 | 2019-04-26 | 广州市锐博生物科技有限公司 | 生成彩色图像的系统及高通量测序仪 |
TW202043450A (zh) | 2018-11-15 | 2020-12-01 | 中國商深圳華大智造科技有限公司 | 用於集成傳感器盒的系統與方法 |
WO2020108588A1 (en) | 2018-12-01 | 2020-06-04 | Mgi Tech Co., Ltd. | Methods and structures to improve light collection efficiency in biosensors |
US10512911B1 (en) | 2018-12-07 | 2019-12-24 | Ultima Genomics, Inc. | Implementing barriers for controlled environments during sample processing and detection |
US11649485B2 (en) | 2019-01-06 | 2023-05-16 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
US10852518B1 (en) * | 2019-03-14 | 2020-12-01 | Ultima Genomics, Inc. | Methods, devices, and systems for analyte detection and analysis |
US11118223B2 (en) | 2019-03-14 | 2021-09-14 | Ultima Genomics, Inc. | Methods, devices, and systems for analyte detection and analysis |
US10900078B2 (en) | 2019-03-14 | 2021-01-26 | Ultima Genomics, Inc. | Methods, devices, and systems for analyte detection and analysis |
US10830703B1 (en) | 2019-03-14 | 2020-11-10 | Ultima Genomics, Inc. | Methods, devices, and systems for analyte detection and analysis |
CN109745934B (zh) * | 2019-03-18 | 2023-11-21 | 中国人民解放军军事科学院军事医学研究院 | 一种阵列式合成装置及喷墨合成仪 |
SG11202111878RA (en) | 2019-05-31 | 2021-11-29 | 10X Genomics Inc | Method of detecting target nucleic acid molecules |
EP4055185A1 (en) | 2019-11-08 | 2022-09-14 | 10X Genomics, Inc. | Spatially-tagged analyte capture agents for analyte multiplexing |
WO2021092433A2 (en) | 2019-11-08 | 2021-05-14 | 10X Genomics, Inc. | Enhancing specificity of analyte binding |
FI3891300T3 (fi) | 2019-12-23 | 2023-05-10 | 10X Genomics Inc | Menetelmät spatiaalista analyysiä varten rna-templatoitua ligaatiota käyttäen |
US11732299B2 (en) | 2020-01-21 | 2023-08-22 | 10X Genomics, Inc. | Spatial assays with perturbed cells |
US11702693B2 (en) | 2020-01-21 | 2023-07-18 | 10X Genomics, Inc. | Methods for printing cells and generating arrays of barcoded cells |
US11821035B1 (en) | 2020-01-29 | 2023-11-21 | 10X Genomics, Inc. | Compositions and methods of making gene expression libraries |
US11898205B2 (en) | 2020-02-03 | 2024-02-13 | 10X Genomics, Inc. | Increasing capture efficiency of spatial assays |
US11732300B2 (en) | 2020-02-05 | 2023-08-22 | 10X Genomics, Inc. | Increasing efficiency of spatial analysis in a biological sample |
US11835462B2 (en) | 2020-02-11 | 2023-12-05 | 10X Genomics, Inc. | Methods and compositions for partitioning a biological sample |
US11891654B2 (en) | 2020-02-24 | 2024-02-06 | 10X Genomics, Inc. | Methods of making gene expression libraries |
US11926863B1 (en) | 2020-02-27 | 2024-03-12 | 10X Genomics, Inc. | Solid state single cell method for analyzing fixed biological cells |
US11768175B1 (en) | 2020-03-04 | 2023-09-26 | 10X Genomics, Inc. | Electrophoretic methods for spatial analysis |
EP4242325A3 (en) | 2020-04-22 | 2023-10-04 | 10X Genomics, Inc. | Methods for spatial analysis using targeted rna depletion |
EP4153775A1 (en) | 2020-05-22 | 2023-03-29 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
EP4153776A1 (en) | 2020-05-22 | 2023-03-29 | 10X Genomics, Inc. | Spatial analysis to detect sequence variants |
AU2021283184A1 (en) | 2020-06-02 | 2023-01-05 | 10X Genomics, Inc. | Spatial transcriptomics for antigen-receptors |
EP4025692A2 (en) | 2020-06-02 | 2022-07-13 | 10X Genomics, Inc. | Nucleic acid library methods |
WO2021252499A1 (en) | 2020-06-08 | 2021-12-16 | 10X Genomics, Inc. | Methods of determining a surgical margin and methods of use thereof |
AU2021294334A1 (en) | 2020-06-25 | 2023-02-02 | 10X Genomics, Inc. | Spatial analysis of DNA methylation |
US11761038B1 (en) | 2020-07-06 | 2023-09-19 | 10X Genomics, Inc. | Methods for identifying a location of an RNA in a biological sample |
US11926822B1 (en) | 2020-09-23 | 2024-03-12 | 10X Genomics, Inc. | Three-dimensional spatial analysis |
US11827935B1 (en) | 2020-11-19 | 2023-11-28 | 10X Genomics, Inc. | Methods for spatial analysis using rolling circle amplification and detection probes |
WO2022140028A1 (en) | 2020-12-21 | 2022-06-30 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
CN112899152B (zh) * | 2021-02-02 | 2023-11-17 | 中国科学院苏州纳米技术与纳米仿生研究所 | 核酸快速扩增和检测的微流控芯片、检测方法及系统 |
EP4301870A1 (en) | 2021-03-18 | 2024-01-10 | 10X Genomics, Inc. | Multiplex capture of gene and protein expression from a biological sample |
CN112986206B (zh) * | 2021-05-20 | 2021-07-30 | 北京百奥纳芯生物科技有限公司 | 一种检测基因芯片杂交结果的方法 |
WO2023034489A1 (en) | 2021-09-01 | 2023-03-09 | 10X Genomics, Inc. | Methods, compositions, and kits for blocking a capture probe on a spatial array |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5091302A (en) * | 1989-04-27 | 1992-02-25 | The Blood Center Of Southeastern Wisconsin, Inc. | Polymorphism of human platelet membrane glycoprotein iiia and diagnostic and therapeutic applications thereof |
US5632957A (en) * | 1993-11-01 | 1997-05-27 | Nanogen | Molecular biological diagnostic systems including electrodes |
CN1175283A (zh) * | 1994-12-09 | 1998-03-04 | 海塞克公司 | 用于dna测序和dna鉴定的方法和装置 |
CN1250485A (zh) * | 1997-01-16 | 2000-04-12 | 希斯克有限公司 | 检测或定量测定核酸种类的方法和组合物 |
US6472156B1 (en) * | 1999-08-30 | 2002-10-29 | The University Of Utah | Homogeneous multiplex hybridization analysis by color and Tm |
Family Cites Families (152)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4994373A (en) | 1983-01-27 | 1991-02-19 | Enzo Biochem, Inc. | Method and structures employing chemically-labelled polynucleotide probes |
US4719179A (en) | 1984-11-30 | 1988-01-12 | Pharmacia P-L Biochemicals, Inc. | Six base oligonucleotide linkers and methods for their use |
US4883750A (en) | 1984-12-13 | 1989-11-28 | Applied Biosystems, Inc. | Detection of specific sequences in nucleic acids |
US5202231A (en) | 1987-04-01 | 1993-04-13 | Drmanac Radoje T | Method of sequencing of genomes by hybridization of oligonucleotide probes |
US5525464A (en) | 1987-04-01 | 1996-06-11 | Hyseq, Inc. | Method of sequencing by hybridization of oligonucleotide probes |
US5124246A (en) | 1987-10-15 | 1992-06-23 | Chiron Corporation | Nucleic acid multimers and amplified nucleic acid hybridization assays using same |
GB8810400D0 (en) | 1988-05-03 | 1988-06-08 | Southern E | Analysing polynucleotide sequences |
US5700637A (en) | 1988-05-03 | 1997-12-23 | Isis Innovation Limited | Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays |
GB8822228D0 (en) | 1988-09-21 | 1988-10-26 | Southern E M | Support-bound oligonucleotides |
US6346413B1 (en) | 1989-06-07 | 2002-02-12 | Affymetrix, Inc. | Polymer arrays |
US5744101A (en) | 1989-06-07 | 1998-04-28 | Affymax Technologies N.V. | Photolabile nucleoside protecting groups |
US5800992A (en) | 1989-06-07 | 1998-09-01 | Fodor; Stephen P.A. | Method of detecting nucleic acids |
US6416952B1 (en) | 1989-06-07 | 2002-07-09 | Affymetrix, Inc. | Photolithographic and other means for manufacturing arrays |
US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
US5427930A (en) | 1990-01-26 | 1995-06-27 | Abbott Laboratories | Amplification of target nucleic acids using gap filling ligase chain reaction |
CA2036946C (en) | 1990-04-06 | 2001-10-16 | Kenneth V. Deugau | Indexing linkers |
JP3080178B2 (ja) | 1991-02-18 | 2000-08-21 | 東洋紡績株式会社 | 核酸配列の増幅方法およびそのための試薬キット |
JP3085409B2 (ja) | 1991-03-29 | 2000-09-11 | 東洋紡績株式会社 | 標的核酸配列の検出方法およびそのための試薬キット |
US5474796A (en) | 1991-09-04 | 1995-12-12 | Protogene Laboratories, Inc. | Method and apparatus for conducting an array of chemical reactions on a support surface |
US6589726B1 (en) | 1991-09-04 | 2003-07-08 | Metrigen, Inc. | Method and apparatus for in situ synthesis on a solid support |
PT534858E (pt) | 1991-09-24 | 2000-09-29 | Keygene Nv | Amplificacao selectiva de fragmentos de restricao um metodo geral para a obtencao de "impressoes digitais" do adn |
US5403708A (en) | 1992-07-06 | 1995-04-04 | Brennan; Thomas M. | Methods and compositions for determining the sequence of nucleic acids |
GB9214873D0 (en) | 1992-07-13 | 1992-08-26 | Medical Res Council | Process for categorising nucleotide sequence populations |
US5403320A (en) * | 1993-01-07 | 1995-04-04 | Venus Corporation | Bone milling guide apparatus and method |
US5837832A (en) * | 1993-06-25 | 1998-11-17 | Affymetrix, Inc. | Arrays of nucleic acid probes on biological chips |
DE69433487T2 (de) * | 1993-09-27 | 2004-11-25 | Arch Development Corp., Chicago | Methoden und zusammensetzungen zur effizienten nukleinsaeuresequenzierung |
US6401267B1 (en) * | 1993-09-27 | 2002-06-11 | Radoje Drmanac | Methods and compositions for efficient nucleic acid sequencing |
SE9400522D0 (sv) | 1994-02-16 | 1994-02-16 | Ulf Landegren | Method and reagent for detecting specific nucleotide sequences |
US5641658A (en) | 1994-08-03 | 1997-06-24 | Mosaic Technologies, Inc. | Method for performing amplification of nucleic acid with two primers bound to a single solid support |
US6013445A (en) | 1996-06-06 | 2000-01-11 | Lynx Therapeutics, Inc. | Massively parallel signature sequencing by ligation of encoded adaptors |
US5866337A (en) | 1995-03-24 | 1999-02-02 | The Trustees Of Columbia University In The City Of New York | Method to detect mutations in a nucleic acid using a hybridization-ligation procedure |
US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
DE69612013T2 (de) | 1995-11-21 | 2001-08-02 | Univ Yale New Haven | Unimolekulare segmentamplifikation und bestimmung |
US5799637A (en) | 1996-05-01 | 1998-09-01 | Cifuni; Charles G. | Rocket effect sparking plug |
US5851804A (en) | 1996-05-06 | 1998-12-22 | Apollon, Inc. | Chimeric kanamycin resistance gene |
DE19638667C2 (de) | 1996-09-20 | 2001-05-17 | Osram Opto Semiconductors Gmbh | Mischfarbiges Licht abstrahlendes Halbleiterbauelement mit Lumineszenzkonversionselement |
GB9620209D0 (en) | 1996-09-27 | 1996-11-13 | Cemu Bioteknik Ab | Method of sequencing DNA |
US6297006B1 (en) | 1997-01-16 | 2001-10-02 | Hyseq, Inc. | Methods for sequencing repetitive sequences and for determining the order of sequence subfragments |
US6309824B1 (en) | 1997-01-16 | 2001-10-30 | Hyseq, Inc. | Methods for analyzing a target nucleic acid using immobilized heterogeneous mixtures of oligonucleotide probes |
ATE269908T1 (de) | 1997-04-01 | 2004-07-15 | Manteia S A | Methode zur sequenzierung von nukleinsäuren |
US5888737A (en) | 1997-04-15 | 1999-03-30 | Lynx Therapeutics, Inc. | Adaptor-based sequence analysis |
US20040229221A1 (en) | 1997-05-08 | 2004-11-18 | Trustees Of Columbia University In The City Of New York | Method to detect mutations in a nucleic acid using a hybridization-ligation procedure |
WO1999009217A1 (en) | 1997-08-15 | 1999-02-25 | Hyseq, Inc. | Methods and compositions for detection or quantification of nucleic acid species |
US6124120A (en) | 1997-10-08 | 2000-09-26 | Yale University | Multiple displacement amplification |
CA2305449A1 (en) | 1997-10-10 | 1999-04-22 | President & Fellows Of Harvard College | Replica amplification of nucleic acid arrays |
DE29724852U1 (de) | 1997-10-22 | 2005-01-13 | Carl Zeiss Meditec Ag | Vorrichtung zur Formgebung von Objekten |
DE19803936A1 (de) | 1998-01-30 | 1999-08-05 | Patent Treuhand Ges Fuer Elektrische Gluehlampen Mbh | Ausdehnungskompensiertes optoelektronisches Halbleiter-Bauelement, insbesondere UV-emittierende Leuchtdiode und Verfahren zu seiner Herstellung |
US6136537A (en) | 1998-02-23 | 2000-10-24 | Macevicz; Stephen C. | Gene expression analysis |
US6501091B1 (en) | 1998-04-01 | 2002-12-31 | Massachusetts Institute Of Technology | Quantum dot white and colored light emitting diodes |
US6004755A (en) | 1998-04-07 | 1999-12-21 | Incyte Pharmaceuticals, Inc. | Quantitative microarray hybridizaton assays |
US6284497B1 (en) * | 1998-04-09 | 2001-09-04 | Trustees Of Boston University | Nucleic acid arrays and methods of synthesis |
JP4262799B2 (ja) | 1998-04-16 | 2009-05-13 | 平田機工株式会社 | 生タイヤ供給方法 |
US6355419B1 (en) | 1998-04-27 | 2002-03-12 | Hyseq, Inc. | Preparation of pools of nucleic acids based on representation in a sample |
US6255469B1 (en) | 1998-05-06 | 2001-07-03 | New York University | Periodic two and three dimensional nucleic acid structures |
EP1046032A4 (en) * | 1998-05-18 | 2002-05-29 | Univ Washington | LIQUID ANALYSIS CARTRIDGE |
US6316229B1 (en) | 1998-07-20 | 2001-11-13 | Yale University | Single molecule analysis target-mediated ligation of bipartite primers |
US6504180B1 (en) | 1998-07-28 | 2003-01-07 | Imec Vzw And Vrije Universiteit | Method of manufacturing surface textured high-efficiency radiating devices and devices obtained therefrom |
WO2000006770A1 (en) | 1998-07-30 | 2000-02-10 | Solexa Ltd. | Arrayed biomolecules and their use in sequencing |
US6787308B2 (en) | 1998-07-30 | 2004-09-07 | Solexa Ltd. | Arrayed biomolecules and their use in sequencing |
US6232067B1 (en) | 1998-08-17 | 2001-05-15 | The Perkin-Elmer Corporation | Adapter directed expression analysis |
US6653077B1 (en) | 1998-09-04 | 2003-11-25 | Lynx Therapeutics, Inc. | Method of screening for genetic polymorphism |
US6203989B1 (en) * | 1998-09-30 | 2001-03-20 | Affymetrix, Inc. | Methods and compositions for amplifying detectable signals in specific binding assays |
DE69930310T3 (de) * | 1998-12-14 | 2009-12-17 | Pacific Biosciences of California, Inc. (n. d. Ges. d. Staates Delaware), Menlo Park | Kit und methode zur nukleinsäuresequenzierung einzelner moleküle durch polymerase synthese |
US6534293B1 (en) | 1999-01-06 | 2003-03-18 | Cornell Research Foundation, Inc. | Accelerating identification of single nucleotide polymorphisms and alignment of clones in genomic sequencing |
DE60042775D1 (de) | 1999-01-06 | 2009-10-01 | Callida Genomics Inc | Verbesserte sequenzierung mittels hybridisierung durch verwendung von sondengemischen |
GB9901475D0 (en) | 1999-01-22 | 1999-03-17 | Pyrosequencing Ab | A method of DNA sequencing |
US6514768B1 (en) | 1999-01-29 | 2003-02-04 | Surmodics, Inc. | Replicable probe array |
US6410231B1 (en) * | 1999-02-26 | 2002-06-25 | Incyte Genomics, Inc. | SNP detection |
US6506594B1 (en) * | 1999-03-19 | 2003-01-14 | Cornell Res Foundation Inc | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays |
EP1192274A2 (en) | 1999-03-25 | 2002-04-03 | Hyseq, Inc. | Solution-based methods and materials for sequence analysis by hybridization |
DE19915510C1 (de) * | 1999-04-07 | 2000-07-27 | Preh Elektro Feinmechanik | Verfahren zur Herstellung von aus Metall bestehenden Gehäuse- oder Stellteilen |
US20030215821A1 (en) * | 1999-04-20 | 2003-11-20 | Kevin Gunderson | Detection of nucleic acid reactions on bead arrays |
EP1190100B1 (en) | 1999-05-20 | 2012-07-25 | Illumina, Inc. | Combinatorial decoding of random nucleic acid arrays |
US6573369B2 (en) | 1999-05-21 | 2003-06-03 | Bioforce Nanosciences, Inc. | Method and apparatus for solid state molecular analysis |
US6502952B1 (en) | 1999-06-23 | 2003-01-07 | Fred Jack Hartley | Light emitting diode assembly for flashlights |
US7501245B2 (en) | 1999-06-28 | 2009-03-10 | Helicos Biosciences Corp. | Methods and apparatuses for analyzing polynucleotide sequences |
US6504301B1 (en) | 1999-09-03 | 2003-01-07 | Lumileds Lighting, U.S., Llc | Non-incandescent lightbulb package using light emitting diodes |
CA2384838C (en) | 1999-09-13 | 2006-07-18 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
US7211390B2 (en) | 1999-09-16 | 2007-05-01 | 454 Life Sciences Corporation | Method of sequencing a nucleic acid |
WO2001023610A2 (en) | 1999-09-29 | 2001-04-05 | Solexa Ltd. | Polynucleotide sequencing |
US6297016B1 (en) | 1999-10-08 | 2001-10-02 | Applera Corporation | Template-dependent ligation with PNA-DNA chimeric probes |
DE19955747A1 (de) | 1999-11-19 | 2001-05-23 | Osram Opto Semiconductors Gmbh | Optische Halbleitervorrichtung mit Mehrfach-Quantentopf-Struktur |
EP1238105A2 (en) | 1999-12-02 | 2002-09-11 | Molecular Staging Inc. | Generation of single-strand circular dna from linear self-annealing segments |
US6500620B2 (en) | 1999-12-29 | 2002-12-31 | Mergen Ltd. | Methods for amplifying and detecting multiple polynucleotides on a solid phase support |
GB0002389D0 (en) | 2000-02-02 | 2000-03-22 | Solexa Ltd | Molecular arrays |
EP1255865B1 (en) | 2000-02-07 | 2007-04-18 | Illumina, Inc. | Nucleic acid detection method using universal priming |
EP1990428B1 (en) | 2000-02-07 | 2010-12-22 | Illumina, Inc. | Nucleic acid detection methods using universal priming |
US6913884B2 (en) * | 2001-08-16 | 2005-07-05 | Illumina, Inc. | Compositions and methods for repetitive use of genomic DNA |
DE10006738C2 (de) | 2000-02-15 | 2002-01-17 | Osram Opto Semiconductors Gmbh | Lichtemittierendes Bauelement mit verbesserter Lichtauskopplung und Verfahren zu seiner Herstellung |
WO2001062982A2 (en) | 2000-02-25 | 2001-08-30 | Mosaic Technologies, Inc. | Methods for multi-stage solid phase amplification of nucleic acids |
US20020004204A1 (en) | 2000-02-29 | 2002-01-10 | O'keefe Matthew T. | Microarray substrate with integrated photodetector and methods of use thereof |
DE10010638A1 (de) | 2000-03-03 | 2001-09-13 | Osram Opto Semiconductors Gmbh | Verfahren zur Herstellung eines lichtabstrahlenden Halbleiterkörpers mit Lumineszenzkonversionselement |
US6413722B1 (en) | 2000-03-22 | 2002-07-02 | Incyte Genomics, Inc. | Polymer coated surfaces for microarray applications |
DE10020464A1 (de) | 2000-04-26 | 2001-11-08 | Osram Opto Semiconductors Gmbh | Strahlungsemittierendes Halbleiterbauelement auf GaN-Basis |
DE10026254A1 (de) | 2000-04-26 | 2001-11-08 | Osram Opto Semiconductors Gmbh | Lumineszenzdiodenchip mit einer auf GaN basierenden strahlungsemittierenden Epitaxieschichtenfolge |
US6878563B2 (en) | 2000-04-26 | 2005-04-12 | Osram Gmbh | Radiation-emitting semiconductor element and method for producing the same |
EP1286226B1 (en) | 2000-05-25 | 2008-02-27 | Fuji Xerox Co., Ltd. | Toner and image forming method |
TWI292227B (en) | 2000-05-26 | 2008-01-01 | Osram Opto Semiconductors Gmbh | Light-emitting-dioed-chip with a light-emitting-epitaxy-layer-series based on gan |
US20020017652A1 (en) | 2000-08-08 | 2002-02-14 | Stefan Illek | Semiconductor chip for optoelectronics |
US6635363B1 (en) | 2000-08-21 | 2003-10-21 | General Electric Company | Phosphor coating with self-adjusting distance from LED chip |
US6429460B1 (en) | 2000-09-28 | 2002-08-06 | United Epitaxy Company, Ltd. | Highly luminous light emitting device |
US20020084745A1 (en) | 2000-12-29 | 2002-07-04 | Airma Optoelectronics Corporation | Light emitting diode with light conversion by dielectric phosphor powder |
US20020096254A1 (en) | 2001-01-22 | 2002-07-25 | Michael Kober | Optical device module and method of fabrication |
DK1370690T3 (da) | 2001-03-16 | 2012-07-09 | Kalim Mir | Arrays og fremgangsmåder til anvendelse heraf |
US6777187B2 (en) | 2001-05-02 | 2004-08-17 | Rubicon Genomics, Inc. | Genome walking by selective amplification of nick-translate DNA library and amplification from complex mixtures of templates |
JP2002368263A (ja) | 2001-06-06 | 2002-12-20 | Toyoda Gosei Co Ltd | Iii族窒化物系化合物半導体発光素子 |
TW543128B (en) | 2001-07-12 | 2003-07-21 | Highlink Technology Corp | Surface mounted and flip chip type LED package |
EP1437776B1 (en) | 2001-10-12 | 2011-09-21 | Nichia Corporation | Light emitting device and method for manufacture thereof |
GB2382137A (en) | 2001-11-20 | 2003-05-21 | Mats Gullberg | Nucleic acid enrichment |
JP4055405B2 (ja) | 2001-12-03 | 2008-03-05 | ソニー株式会社 | 電子部品及びその製造方法 |
US7011945B2 (en) | 2001-12-21 | 2006-03-14 | Eastman Kodak Company | Random array of micro-spheres for the analysis of nucleic acids |
US20040002090A1 (en) | 2002-03-05 | 2004-01-01 | Pascal Mayer | Methods for detecting genome-wide sequence variations associated with a phenotype |
JP3707688B2 (ja) | 2002-05-31 | 2005-10-19 | スタンレー電気株式会社 | 発光装置およびその製造方法 |
US20050019776A1 (en) | 2002-06-28 | 2005-01-27 | Callow Matthew James | Universal selective genome amplification and universal genotyping system |
DE10234977A1 (de) | 2002-07-31 | 2004-02-12 | Osram Opto Semiconductors Gmbh | Strahlungsemittierendes Dünnschicht-Halbleiterbauelement auf GaN-Basis |
AU2003267583A1 (en) | 2002-09-19 | 2004-04-08 | The Chancellor, Master And Scholars Of The University Of Oxford | Molecular arrays and single molecule detection |
US20040058330A1 (en) * | 2002-09-20 | 2004-03-25 | Prokaria, Ltd. | Methods of use for thermostable RNA ligases |
DE10245628A1 (de) | 2002-09-30 | 2004-04-15 | Osram Opto Semiconductors Gmbh | Elektromagnetische Strahlung emittierender Halbleiterchip und Verfahren zu dessen Herstellung |
US7459273B2 (en) | 2002-10-04 | 2008-12-02 | Affymetrix, Inc. | Methods for genotyping selected polymorphism |
WO2004034028A2 (en) * | 2002-10-09 | 2004-04-22 | The Board Of Trustees Of The University Of Illinois | Microfluidic systems and components |
US20040086869A1 (en) * | 2002-10-31 | 2004-05-06 | Schembri Carol T. | Device having multiple molecular arrays |
JP4395133B2 (ja) | 2002-12-20 | 2010-01-06 | カリパー・ライフ・サイエンシズ・インク. | Dnaの単一分子増幅および検出 |
AU2004254552B2 (en) | 2003-01-29 | 2008-04-24 | 454 Life Sciences Corporation | Methods of amplifying and sequencing nucleic acids |
JP2006517798A (ja) | 2003-02-12 | 2006-08-03 | イェニソン スベンスカ アクティエボラーグ | 核酸配列用の方法及び手段 |
AU2004214891B2 (en) | 2003-02-26 | 2010-01-07 | Complete Genomics, Inc. | Random array DNA analysis by hybridization |
WO2005029040A2 (en) | 2003-09-18 | 2005-03-31 | Parallele Biosciences, Inc. | System and methods for enhancing signal-to-noise ratios of microarray-based measurements |
GB0324456D0 (en) | 2003-10-20 | 2003-11-19 | Isis Innovation | Parallel DNA sequencing methods |
EP2202322A1 (en) | 2003-10-31 | 2010-06-30 | AB Advanced Genetic Analysis Corporation | Methods for producing a paired tag from a nucleic acid sequence and methods of use thereof |
US7169560B2 (en) | 2003-11-12 | 2007-01-30 | Helicos Biosciences Corporation | Short cycle methods for sequencing polynucleotides |
WO2005073410A2 (en) | 2004-01-28 | 2005-08-11 | 454 Corporation | Nucleic acid amplification with continuous flow emulsion |
GB0402895D0 (en) | 2004-02-10 | 2004-03-17 | Solexa Ltd | Arrayed polynucleotides |
EP2248911A1 (en) | 2004-02-19 | 2010-11-10 | Helicos Biosciences Corporation | Methods and kits for analyzing polynucleotide sequences |
CA2557841A1 (en) | 2004-02-27 | 2005-09-09 | President And Fellows Of Harvard College | Polony fluorescent in situ sequencing beads |
US20050214840A1 (en) | 2004-03-23 | 2005-09-29 | Xiangning Chen | Restriction enzyme mediated method of multiplex genotyping |
GB2413796B (en) | 2004-03-25 | 2006-03-29 | Global Genomics Ab | Methods and means for nucleic acid sequencing |
US20050260609A1 (en) | 2004-05-24 | 2005-11-24 | Lapidus Stanley N | Methods and devices for sequencing nucleic acids |
US20070117104A1 (en) | 2005-11-22 | 2007-05-24 | Buzby Philip R | Nucleotide analogs |
WO2006007207A2 (en) | 2004-05-25 | 2006-01-19 | Helicos Biosciences Corporation | Methods and devices for nucleic acid sequence determination |
US7276720B2 (en) | 2004-07-19 | 2007-10-02 | Helicos Biosciences Corporation | Apparatus and methods for analyzing samples |
US20060012793A1 (en) | 2004-07-19 | 2006-01-19 | Helicos Biosciences Corporation | Apparatus and methods for analyzing samples |
WO2006073504A2 (en) | 2004-08-04 | 2006-07-13 | President And Fellows Of Harvard College | Wobble sequencing |
GB0422551D0 (en) | 2004-10-11 | 2004-11-10 | Univ Liverpool | Labelling and sequencing of nucleic acids |
CA2588122A1 (en) | 2004-11-16 | 2006-05-26 | Helicos Biosciences Corporation | Tirf single molecule analysis and method of sequencing nucleic acids |
EP1844162B1 (en) | 2005-02-01 | 2014-10-15 | Applied Biosystems, LLC | Method for identifying a sequence in a polynucleotide |
SG162795A1 (en) | 2005-06-15 | 2010-07-29 | Callida Genomics Inc | Single molecule arrays for genetic and chemical analysis |
EP1907591A2 (en) | 2005-07-28 | 2008-04-09 | Helicos Biosciences Corporation | Consecutive base single molecule sequencing |
US7666593B2 (en) | 2005-08-26 | 2010-02-23 | Helicos Biosciences Corporation | Single molecule sequencing of captured nucleic acids |
WO2007087310A2 (en) | 2006-01-23 | 2007-08-02 | Population Genetics Technologies Ltd. | Nucleic acid analysis using sequence tokens |
WO2007092538A2 (en) | 2006-02-07 | 2007-08-16 | President And Fellows Of Harvard College | Methods for making nucleotide probes for sequencing and synthesis |
SG10201405158QA (en) | 2006-02-24 | 2014-10-30 | Callida Genomics Inc | High throughput genome sequencing on dna arrays |
US7910354B2 (en) | 2006-10-27 | 2011-03-22 | Complete Genomics, Inc. | Efficient arrays of amplified polynucleotides |
US20090111705A1 (en) | 2006-11-09 | 2009-04-30 | Complete Genomics, Inc. | Selection of dna adaptor orientation by hybrid capture |
-
2004
- 2004-02-26 AU AU2004214891A patent/AU2004214891B2/en not_active Expired
- 2004-02-26 CN CN201310140881.2A patent/CN103396933B/zh not_active Expired - Lifetime
- 2004-02-26 US US10/547,214 patent/US8105771B2/en active Active
- 2004-02-26 CN CN201310141641.4A patent/CN103289893B/zh not_active Expired - Lifetime
- 2004-02-26 CN CN201310140862.XA patent/CN103397082B/zh not_active Expired - Lifetime
- 2004-02-26 EP EP04715167.5A patent/EP1601791B1/en not_active Expired - Lifetime
- 2004-02-26 CA CA2930400A patent/CA2930400A1/en not_active Abandoned
- 2004-02-26 EP EP10176830A patent/EP2365095A1/en not_active Withdrawn
- 2004-02-26 JP JP2006503913A patent/JP4691014B2/ja not_active Expired - Lifetime
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Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5091302A (en) * | 1989-04-27 | 1992-02-25 | The Blood Center Of Southeastern Wisconsin, Inc. | Polymorphism of human platelet membrane glycoprotein iiia and diagnostic and therapeutic applications thereof |
US5632957A (en) * | 1993-11-01 | 1997-05-27 | Nanogen | Molecular biological diagnostic systems including electrodes |
CN1164894A (zh) * | 1994-09-09 | 1997-11-12 | 内诺金公司 | 自动分子生物学诊断系统 |
CN1175283A (zh) * | 1994-12-09 | 1998-03-04 | 海塞克公司 | 用于dna测序和dna鉴定的方法和装置 |
CN1250485A (zh) * | 1997-01-16 | 2000-04-12 | 希斯克有限公司 | 检测或定量测定核酸种类的方法和组合物 |
US6472156B1 (en) * | 1999-08-30 | 2002-10-29 | The University Of Utah | Homogeneous multiplex hybridization analysis by color and Tm |
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