CN103308587A - Hollow fiber electrophoresis gel imaging system - Google Patents
Hollow fiber electrophoresis gel imaging system Download PDFInfo
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- CN103308587A CN103308587A CN2013102080595A CN201310208059A CN103308587A CN 103308587 A CN103308587 A CN 103308587A CN 2013102080595 A CN2013102080595 A CN 2013102080595A CN 201310208059 A CN201310208059 A CN 201310208059A CN 103308587 A CN103308587 A CN 103308587A
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Abstract
The invention relates to a hollow fiber electrophoresis gel imaging system. The hollow fiber electrophoresis gel imaging system comprises a CCD (Charge Coupled Device) or CMOS (Complementary Metal-Oxide-Semiconductor) camera capable of focusing, a stepping motor, high-voltage power supply electrodes, a first light filter, hollow fibers, a touch-screen LED (light-emitting diode) display, a second light filter, ultraviolet lamps and an imaging dark box, wherein one or multiple hollow fibers are arranged between the two high-voltage power supply electrodes, the camera is mounted above the hollow fiber and is driven to transversely and horizontally move by the stepping motor, and the first light filter is mounted at the bottom of the camera; the second light filter is arranged below the hollow fibers, and the ultraviolet lamps are arranged below the second light filter; the touch-screen LED display is used for controlling the imaging system; gel is filled in the hollow fibers, a to-be-measured biological or drug molecule sample is dropwise added at one ends of the hollow fibers, and the hollow fibers are taken as lanes of electrophoresis and conductive channels of light beams to carry out electrophoresis gel imaging and molecular spectrum analysis and detection on the sample. The hollow fiber electrophoresis gel imaging system has the characteristics of small sample dosage, sensitivity, high efficiency, non pollution and the like.
Description
Technical field
The present invention relates to a kind of hollow-core fiber running gel imaging system.
Background technology
The nucleic acid gel electrophoresis is a kind of common method of separation in the molecular biology experiment, evaluation and purification of nucleic acid molecules, probe, amplified production.Its ultimate principle is that gel is put in the electric field, utilize the characteristic that charged nucleic acid moves by gel mesh anode under the pH neutral, nucleic acid fragment big or small behind the electrophoresis appropriate time, that conformation is different will be scalariform and be arranged on the gel diverse location, thereby reach the purpose of separation.The multifactor impacts such as the migration rate of nucleic acid is subject to molecular size, conformation, agarose concentration, applies voltage, electric field, electrophoretic buffer, intercalative dye amount.Just because of this, traditional nucleic acid electrophoresis imaging effect is subjected to kinds of experiments condition and effect of parameters, is difficult to realize the organic unity of repeatability and accuracy.Simultaneously, the common dyes ethidium bromide of nucleic acid electrophoresis (ethidium bromide, EB) is a kind of strong mutagen and moderate toxicity arranged, and uses operationally lack of standardizationly easily to bring adverse influence to health and environment.
Summary of the invention
Defective for prior art exists the purpose of this invention is to provide a kind of hollow-core fiber running gel imaging system, and the detection of light harvesting Epidemiological Analysis and electrophoresis and integrated are realized integrated detection.Native system is collection nucleic acid electrophoresis, optical imagery, data collection and analysis, " one-stop " experimental study platform of detection of biological nucleic acid molecules, be used for measuring biomedical molecule electricity, optics, and gel images, to the multi-faceted detection of molecule.
For achieving the above object, the present invention adopts following technical scheme:
A kind of hollow-core fiber running gel imaging system comprises CCD or the CMOS camera that can focus, stepping motor, high-voltage power supply electrode, the first optical filter, hollow-core fiber, touch-screen light-emitting diode display, the second optical filter, uviol lamp and imaging camera bellows; Described two high-voltage power supply electrode intermediate arrangement have one or more hollow-core fiber, and described camera is installed in the top of hollow-core fiber, drive left and right horizontal by stepping motor and move, and the bottom of camera is equipped with the first optical filter; Described the second optical filter is arranged in the below of hollow-core fiber, and the second optical filter below is uviol lamp; Described camera, stepping motor, the high-voltage power supply electrode, the first optical filter, hollow-core fiber, the second optical filter and uviol lamp place described imaging camera bellows, and described touch-screen light-emitting diode display is installed in the imaging camera bellows outside, is used for controlling imaging system; Be gel in the hollow-core fiber, biology to be measured or drug molecule sample drop are added in an end of hollow-core fiber, utilize hollow-core fiber as the swimming lane of electrophoresis and the passage of light beam conduction, and sample is carried out running gel imaging and Molecular Spectral Analysis detection.
Compared with prior art, the present invention has following outstanding substantive distinguishing features and advantage significantly:
Hollow-core fiber running gel imaging system of the present invention can be from optical imagery, Molecular Spectral Analysis, and the many levels such as electrophoresis electric field separates are detection molecules simultaneously.The cardinal principle of this system is still by charged nucleic acid molecules separates in the electrophoretic buffer classification, difference is that the carrier of nucleic acid electrophoresis is hollow optic fibre, this optical fiber can conduct light beam on the one hand, the conduction light beam can carry out detection analysis through the information of Carrier molecule behind the molecule, on the other hand can be in its hollow middle separation of molecule being carried out electric field action etc. as the swimming lane of electrophoresis, can effectively overcome nucleic acid molecules in electrophoresis process, be subjected to tube wall dissociate because of element affect, such as surface charge, surfactant, protein etc.Dye molecule EB directly is poured in the optical fiber tube, has avoided and the contacting of the external world, and can effectively reduce pollution.Output signal is by the light signal collection system, and the fluorescence intensity of ultraviolet irradiation embedded nuclear acid molecule dyestuff EB gained is converted to digital signal, can reduce common background interference in the traditional core acid gel electrophoresis.The characteristics such as in sum, it is few that this system has amount of samples, sensitive efficient, pollution-free.
Description of drawings
Fig. 1 is the overall design schematic diagram of the new type gel imaging system that the present invention relates to.
Embodiment
Details are as follows by reference to the accompanying drawings for the preferred embodiments of the present invention:
Referring to Fig. 1, a kind of hollow-core fiber running gel imaging system comprises CCD or the CMOS camera 1 that can focus, stepping motor 2, high-voltage power supply electrode 3, the first optical filters 4, hollow-core fiber 5, touch-screen light-emitting diode display 6, the second optical filters 7, uviol lamp 8 and imaging camera bellows 9; Described two high-voltage power supply electrode 3 intermediate arrangement have one or more hollow-core fiber 5, and described camera 1 is installed in the top of hollow-core fiber 5, drive left and right horizontal by stepping motor 2 and move, and the bottom of camera 1 is equipped with the first optical filter 4; Described the second optical filter 7 is arranged in the below of hollow-core fiber 5, and the second optical filter 7 belows are uviol lamp 8; Described camera 1, stepping motor 2, high-voltage power supply electrode 3, the first optical filters 4, hollow-core fiber 5, the second optical filters 7 and uviol lamp 8 place described imaging camera bellows 9, and described touch-screen light-emitting diode display 6 is installed in imaging camera bellows 9 outsides, is used for controlling imaging system; Be gel in the hollow-core fiber 5, biology to be measured or drug molecule sample drop are added in an end of hollow-core fiber 5, utilize hollow-core fiber 5 as the swimming lane of electrophoresis and the passage of light beam conduction, and sample is carried out running gel imaging and Molecular Spectral Analysis detection.
Camera 1 adopts Sony corporation of Japan APR-2110Z adjustable focus CCD camera in the present embodiment; Adopting diameter is the hollow-core fiber 5 of 420 μ m, totally 20; High-voltage power voltage is 1kv; The gel that adopts is Ago-Gel; Tested sample is that DNA marker is the PCR fragment of a bacteriophage in dna molecular amount reference material and the phage display library.After sample adds and electrophoresis powers on, collect clearly gel imaging picture.
Claims (1)
1. hollow-core fiber running gel imaging system, it is characterized in that, comprise CCD or the CMOS camera (1) that to focus, stepping motor (2), high-voltage power supply electrode (3), the first optical filter (4), hollow-core fiber (5), touch-screen light-emitting diode display (6), the second optical filter (7), uviol lamp (8) and imaging camera bellows (9); Described two high-voltage power supply electrodes (3) intermediate arrangement has one or more hollow-core fiber (5), described camera (1) is installed in the top of hollow-core fiber (5), drive left and right horizontal by stepping motor (2) and move, the bottom of camera (1) is equipped with the first optical filter (4); Described the second optical filter (7) is arranged in the below of hollow-core fiber (5), and the second optical filter (7) below is uviol lamp (8); Described camera (1), stepping motor (2), high-voltage power supply electrode (3), the first optical filter (4), hollow-core fiber (5), the second optical filter (7) and uviol lamp (8) place described imaging camera bellows (9), and described touch-screen light-emitting diode display (6) is installed in imaging camera bellows (9) outside, is used for controlling imaging system; Be gel in the hollow-core fiber (5), biology to be measured or drug molecule sample drop are added in an end of hollow-core fiber (5), utilize hollow-core fiber (5) as the swimming lane of electrophoresis and the passage of light beam conduction, sample is carried out running gel imaging and Molecular Spectral Analysis detection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310208059.5A CN103308587B (en) | 2013-05-30 | 2013-05-30 | Hollow fiber electrophoresis gel imaging system |
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| CN201310208059.5A CN103308587B (en) | 2013-05-30 | 2013-05-30 | Hollow fiber electrophoresis gel imaging system |
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| CN103308587A true CN103308587A (en) | 2013-09-18 |
| CN103308587B CN103308587B (en) | 2014-12-31 |
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| CN201310208059.5A Expired - Fee Related CN103308587B (en) | 2013-05-30 | 2013-05-30 | Hollow fiber electrophoresis gel imaging system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107860813A (en) * | 2017-11-16 | 2018-03-30 | 上海仪龙生物科技有限公司 | A kind of PhastGel electrophoresis and real-time imaging devices |
| CN111812091A (en) * | 2020-06-28 | 2020-10-23 | 上海交通大学 | Chip gel electrophoresis and on-line UV-VIS imaging detection device thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5324401A (en) * | 1993-02-05 | 1994-06-28 | Iowa State University Research Foundation, Inc. | Multiplexed fluorescence detector system for capillary electrophoresis |
| JPH09281078A (en) * | 1996-04-09 | 1997-10-31 | Hitachi Electron Eng Co Ltd | Dna base sequence determining apparatus |
| US20010054554A1 (en) * | 2000-04-13 | 2001-12-27 | Janusz Pawliszyn | Measurement of fluorescence using capillary isoelectric focusing |
| CN1865932A (en) * | 2005-05-19 | 2006-11-22 | 清华大学 | Fluorometric detector for micro-fluidic chip system |
| CN1869662A (en) * | 2006-05-15 | 2006-11-29 | 清华大学 | Multi-channel column imaging fluorescent detector |
-
2013
- 2013-05-30 CN CN201310208059.5A patent/CN103308587B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5324401A (en) * | 1993-02-05 | 1994-06-28 | Iowa State University Research Foundation, Inc. | Multiplexed fluorescence detector system for capillary electrophoresis |
| JPH09281078A (en) * | 1996-04-09 | 1997-10-31 | Hitachi Electron Eng Co Ltd | Dna base sequence determining apparatus |
| US20010054554A1 (en) * | 2000-04-13 | 2001-12-27 | Janusz Pawliszyn | Measurement of fluorescence using capillary isoelectric focusing |
| CN1865932A (en) * | 2005-05-19 | 2006-11-22 | 清华大学 | Fluorometric detector for micro-fluidic chip system |
| CN1869662A (en) * | 2006-05-15 | 2006-11-29 | 清华大学 | Multi-channel column imaging fluorescent detector |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107860813A (en) * | 2017-11-16 | 2018-03-30 | 上海仪龙生物科技有限公司 | A kind of PhastGel electrophoresis and real-time imaging devices |
| CN111812091A (en) * | 2020-06-28 | 2020-10-23 | 上海交通大学 | Chip gel electrophoresis and on-line UV-VIS imaging detection device thereof |
| CN111812091B (en) * | 2020-06-28 | 2023-09-05 | 上海交通大学 | Chip gel electrophoresis and online UV-VIS imaging detection device thereof |
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| CN103308587B (en) | 2014-12-31 |
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Granted publication date: 20141231 Termination date: 20190530 |