CN103308349A - Micro-dialysis sensor - Google Patents
Micro-dialysis sensor Download PDFInfo
- Publication number
- CN103308349A CN103308349A CN2013102298519A CN201310229851A CN103308349A CN 103308349 A CN103308349 A CN 103308349A CN 2013102298519 A CN2013102298519 A CN 2013102298519A CN 201310229851 A CN201310229851 A CN 201310229851A CN 103308349 A CN103308349 A CN 103308349A
- Authority
- CN
- China
- Prior art keywords
- sensor
- microdialysis
- electrode
- conduit
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
Abstract
The invention discloses a micro-dialysis sensor which comprises a micro-dialysis membrane guide pipe (6), a micro-dialysis liquid guide pipe (5), a liquid inlet channel guide pipe (7) and a liquid outlet channel guide pipe (8), wherein the micro-dialysis membrane guide pipe (6) is positioned at the top end of the lower part of the micro-dialysis liquid guide pipe (5); the liquid inlet channel guide pipe (7) and the liquid outlet channel guide pipe (8) are positioned in the micro-dialysis liquid guide pipe (5); a needle electrode or a sensor is arranged in the micro-dialysis liquid guide pipe (5). The micro-dialysis sensor is applied to detecting biochemical substance concentration or electrophysiology, has the functions of observing the concentration index of the specific biochemical substance in real time and has the unparalleled advantage for tests in which detection data are required to be rapidly acquired; the micro-dialysis sensor has the function of collecting micro-dialysis liquid, can be used for detecting and verifying other later-stage biological indexes and has the function of detecting electrophysiological function signals in real time, so that an experimenter can simultaneously know the coordination of the electrophysiological and biochemical functions.
Description
Technical field
The present invention relates to a kind of microdialysis sensor, real-time detection of biological is active when specifically being used for biological living sampling or administration.
Background technology
The tradition micro dialysis needle is a kind of device for biological living sampling and administration, and this device will be fixed in particular experiment position on the biological living in experiment.Dialysis needle mainly is by the liquid-inlet passage, and liquid outlet passage and microdialysis film pipeline form, and in experimentation, dislysate will be filled with by the liquid-inlet passage and be poured in the microdialysis film pipeline, then flows out by liquid outlet passage to be collected.In microdialysis film pipeline, because the concentration gradient acting force, the different high concentration materials in dislysate and the biosome in the liquid can enter another liquid phase by dialysis membrane, the function that therefore can reach topical and measure the interior biochemical substances concentration of body.
Be in the Chinese patent of CN1358994A at publication number, disclose a kind of sensor type microdialysis biological living sampling device, on the basis of traditional microdialysis film, stay pipe, feed tube, drain pipe, lower external face at stay pipe arranges one deck conduction sensing membrane that one deck is made by gold, platinum or palladium metal, and at the outside surface of stay pipe one layer insulating is set.Overcome the limitation that conventional microdialysis sampling device can not detect gas in the biological tissue or other biochemical substances content simultaneously with this, so that can when obtaining microdialysate, detect the sensor-type microdialysis sampling device of organizing gas and other biochemical substances content in the inner cell external environment.
To sum up, existing Microdialysis sgstem for the biological living experiment remains in following several point defects:
1) can not immediately reflect fast the defective of biological living other biological activity index: measure biochemical substances concentration and need certain hour to make dislysate reach minimum measurement volumes by collecting dislysate, so the Biological indicators reflection has a timing difference.
2) can't reflect simultaneously the other biological characteristic performance, such as electrophysiological change.
Summary of the invention
For deficiency of the prior art, the invention provides a kind of microdialysis sensor, to incorporate the method for microelectrode or microsensor into, so that when utilizing the sampling of microdialysis principle or administration, can detect in real time biochemical substances concentration and electric physiology.
A kind of microdialysis sensor, comprise microdialysis membrane duct, microdialysate conduit, liquid-inlet passage catheter and liquid outlet passage conduit, the microdialysis membrane duct is positioned at the lower tip of microdialysate conduit, liquid-inlet passage catheter and liquid outlet passage conduit are positioned within the microdialysate conduit, wherein, in the microdialysate conduit, also be provided with needle electrode or sensor.
Preferably, in the microdialysate conduit sensor electrode is set, described sensor electrode contains in the electrode insulation sleeve pipe, and the sensor electrode upper end is connected to electrode contact, and electrode contact further links to each other with the electric signal gatherer with the electromotive force device.And microdialysate conduit outside is wound with the reference electrode that is communicated with electrode contact.
Preferably, be arranged with the liquid-inlet access sheath on the upper end expose portion of described liquid-inlet passage catheter, be arranged with the liquid outlet passage sleeve pipe on the upper end expose portion of liquid outlet passage conduit.
Preferably, also be provided with microdialysis sensor fixed body on the described microdialysis sensor.
Preferably, described sensor electrode is made by platinum, gold or these metals of platinum plating tungsten filament.And the outside of sensor electrode scribbles dglutamic oxidase layer, choline oxidase layer, Nafion layer, glucose oxidase layer, lactose oxidase layer or pyruvate oxidase layer.Certainly, can also be with the absolute electrode in the alternative such scheme of a plurality of electrodes of microarray formula.
Compared with prior art, microdialysis sensor of the present invention has following some advantage:
1) structure that mutually combines of microdialysis and microsensor technology is compact and do not change separately
Measuring ability;
2) can be combined with a plurality of microsensors, so that detection material is diversified, at same biology
Obtain simultaneously various functional parameter in the body, have very great significance for relation and the interaction observed between the various functions index, and save experiment fees and time.
3) real-time monitored particular organisms chemical substance concentration index is detected fast for needs
The experiment of data has incomparable advantage.Collect simultaneously microdialysate, be used for the detection of later stage other biological index and examine.
4) detect in real time the electrophysiological function signal, can make the experimenter understand simultaneously electric physiology with biological
The harmony of chemical functional.
Description of drawings
Fig. 1 is the structural representation of microdialysis sensor of the present invention.
Number in the figure explanation: 1. liquid-inlet access sheath, 2. liquid outlet passage sleeve pipe, 3. electrode contact, 4. microdialysis sensor fixed body, 5. microdialysate conduit, 6. microdialysis membrane duct, 7. liquid-inlet passage catheter, 8. liquid outlet passage conduit, 9. electrode insulation sleeve pipe, 10. sensor electrode, 11. reference electrodes.
Embodiment
A kind of microdialysis sensor of the present invention is comprised of three parts, and topmost part is comprised of liquid-inlet access sheath 1, liquid outlet passage sleeve pipe 2 and electrode contact 3, is to be exposed to the part that biological living is outer and be connected with other instruments in the experiment.Liquid-inlet access sheath 1 is connected with the micro liquid sampling pump, and from then on dislysate enters the microdialysis sensor; Liquid outlet passage sleeve pipe 2 is connected with the microdialysate gatherer, collects dislysate with biochemical substances in the body with this; Electrode contact 3 links to each other with the electric signal gatherer with the electromotive force device, is used for collecting the raw electrical signal of physiology or biochemical substances concentration.
This device center section is main part, is the part that each pipeline connects, and can be close to organism surface in experiment.The reference electrode 11 that contains in electrode insulation sleeve pipe 9 sensor electrode 10 and be wrapped in outside the microdialysate conduit 5 will be connected on the electrode contact, and reference electrode 11 can be selected silver-silver chloride electrode; Liquid-inlet passage catheter 7 and liquid outlet passage conduit 8 will be connected respectively to liquid-inlet access sheath 1 and liquid outlet passage sleeve pipe 2; The liquid entrance passage catheter that these are fine and sensor electrode 10 will close up and be received in the microdialysate conduit 5, and head is used glue sealing; Spiral fashion is twined and is fixed on the reference electrode 11 of microdialysate conduit 5 outsides as optional part with glue; All these pipelines connect will be fixed and be imbedded in by hard material to be in the microdialysis sensor fixed body 4 of matrix, to be used for reinforcing whole device, and is beneficial to the experimenter and uses operation.
This installs last part is microdialysate conduit 5 bottoms and microdialysis membrane duct 6 parts, and they externally are connected and fixed with glue, and inner hollow space links up, and will be full of dislysate in the experiment.This part in the meeting implantable bioartificial body, is used for gathering every physiological and biochemical index in the biosome in experiment.The liquid-inlet passage catheter will one in microdialysate conduit bottom microdialysis membrane duct 6, the conduit mouth of liquid outlet passage conduit 8 then rests on microdialysate conduit middle and upper part; Sensor electrode 10 also is arranged in hollow conduit, made by materials such as metal such as platinum, gold or platinum plating tungsten filaments, can do different disposal to the microsensor surface according to different requirement of experiment, comprise the biological indicator that is not limited to following several processing mode and detects:
1) sensor surface scribbles the dglutamic oxidase layer, detects content of glutamic acid by electrochemical method;
2) sensor surface scribbles the choline oxidase layer, detects acetyl choline content by electrochemical method;
3) sensor surface scribbles glucose oxidase layer, detects glucose content by electrochemical method;
4) sensor surface scribbles the lactose oxidase layer, detects lactose content by electrochemical method;
5) sensor surface scribbles the pyruvate oxidase layer, detects pyruvic acid content by electrochemical method;
6) sensor surface scribbles the Nafion layer, detects DOPAMINE CONTENT IN RABBIT by electrochemical method;
7) keep the sensor gold metal surface, can be used as metal microelectrode for detection of electricity physiological signal.
And also can change the sensor electrical number of poles according to requirement of experiment, for example can utilize a plurality of electrode spread of microarray formula to substitute absolute electrode in the present invention.
The above; it only is better case study on implementation of the present invention; be not that the present invention is imposed any restrictions; every similar mode of any simple modification, change, employing that essence is done above embodiment according to the present invention substitutes and the variation of equivalent structure, all still belongs in the protection domain of technical solution of the present invention.
Claims (10)
1. microdialysis sensor, comprise microdialysis membrane duct (6), microdialysate conduit (5), liquid-inlet passage catheter (7) and liquid outlet passage conduit (8), microdialysis membrane duct (6) is positioned at the lower tip of microdialysate conduit (5), liquid-inlet passage catheter (7) and liquid outlet passage conduit (8) are positioned within the microdialysate conduit (5), it is characterized in that, in microdialysate conduit (5), be provided with needle electrode or sensor.
2. microdialysis sensor according to claim 1 is characterized in that, sensor electrode (10) is set in microdialysate conduit (5), and described sensor electrode (10) contains in electrode insulation sleeve pipe (9).
3. microdialysis sensor according to claim 2 is characterized in that, described sensor electrode (10) upper end is connected to electrode contact (3), and electrode contact (3) further links to each other with the electric signal gatherer with the electromotive force device.
4. microdialysis sensor according to claim 3 is characterized in that, described microdialysate conduit (5) outside is wound with the reference electrode (11) that is communicated with electrode contact (3).
5. microdialysis sensor according to claim 1, it is characterized in that, be arranged with liquid-inlet access sheath (1) on the upper end expose portion of described liquid-inlet passage catheter (7), be arranged with liquid outlet passage sleeve pipe (2) on the upper end expose portion of liquid outlet passage conduit (8).
6. microdialysis sensor according to claim 1 is characterized in that, also is provided with on the described microdialysis sensor for the microdialysis sensor fixed body (4) of reinforcing.
7. each described microdialysis sensor in 6 according to claim 1 is characterized in that, described sensor electrode (10) is made by platinum, gold or these metals of platinum plating tungsten filament.
8. microdialysis sensor according to claim 7 is characterized in that, the outside of described sensor electrode (10) scribbles dglutamic oxidase layer, choline oxidase layer or Nafion layer.
9. microdialysis sensor according to claim 7 is characterized in that, the outside of described sensor electrode (10) scribbles glucose oxidase layer, lactose oxidase layer or pyruvate oxidase layer.
10. microdialysis sensor according to claim 7 is characterized in that, described sensor electrode (10) can be a plurality of electrodes of microarray formula, and scribbles different enzyme layers or Nafion layer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013102298519A CN103308349A (en) | 2013-06-09 | 2013-06-09 | Micro-dialysis sensor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013102298519A CN103308349A (en) | 2013-06-09 | 2013-06-09 | Micro-dialysis sensor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103308349A true CN103308349A (en) | 2013-09-18 |
Family
ID=49133813
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013102298519A Pending CN103308349A (en) | 2013-06-09 | 2013-06-09 | Micro-dialysis sensor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103308349A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103822944A (en) * | 2014-02-12 | 2014-05-28 | 绿谷（集团）有限公司 | Real-time on-line detection method and device of rapid biological signals |
| CN104383631A (en) * | 2014-11-27 | 2015-03-04 | 王俐帧 | Anesthetic needle with measuring device |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1993005701A1 (en) * | 1991-09-20 | 1993-04-01 | Imperial College Of Science, Technology And Medicine | A dialysis electrode device |
| US5607390A (en) * | 1992-12-15 | 1997-03-04 | Institute Of Neurology | Dialysis probe |
| US6013029A (en) * | 1993-10-09 | 2000-01-11 | Korf; Jakob | Monitoring the concentration of a substance or a group of substances in a body fluid |
| US6280604B1 (en) * | 2000-03-10 | 2001-08-28 | Duke University | Electrode materials, systems and methods for the electrochemical detection of nitric oxide |
| CN1358994A (en) * | 2000-12-18 | 2002-07-17 | 中国科学院化学研究所 | Sensor type microdialysis biological living sampling device |
| WO2003003911A2 (en) * | 2001-07-06 | 2003-01-16 | Lukas Schaupp | Measurement of the concentration of substances in living organisms using microdialysis |
| CN101548892A (en) * | 2009-04-29 | 2009-10-07 | 天津市先石光学技术有限公司 | Implanted in-vivo continuous blood sugar monitor |
| CN101642380A (en) * | 2009-09-17 | 2010-02-10 | 华东理工大学 | Direct implantation type microdialysis probe and preparation method thereof |
| CN102504111A (en) * | 2011-11-17 | 2012-06-20 | 中国科学院化学研究所 | Electrode surface modification material, preparation method thereof and application thereof |
| CN102525569A (en) * | 2011-12-28 | 2012-07-04 | 浙江省肿瘤医院 | Array type microdialysis probe |
| CN203365179U (en) * | 2013-06-09 | 2013-12-25 | 覃思 | Microdialysis sensor |
-
2013
- 2013-06-09 CN CN2013102298519A patent/CN103308349A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1993005701A1 (en) * | 1991-09-20 | 1993-04-01 | Imperial College Of Science, Technology And Medicine | A dialysis electrode device |
| US5607390A (en) * | 1992-12-15 | 1997-03-04 | Institute Of Neurology | Dialysis probe |
| US6013029A (en) * | 1993-10-09 | 2000-01-11 | Korf; Jakob | Monitoring the concentration of a substance or a group of substances in a body fluid |
| US6280604B1 (en) * | 2000-03-10 | 2001-08-28 | Duke University | Electrode materials, systems and methods for the electrochemical detection of nitric oxide |
| CN1358994A (en) * | 2000-12-18 | 2002-07-17 | 中国科学院化学研究所 | Sensor type microdialysis biological living sampling device |
| WO2003003911A2 (en) * | 2001-07-06 | 2003-01-16 | Lukas Schaupp | Measurement of the concentration of substances in living organisms using microdialysis |
| CN101548892A (en) * | 2009-04-29 | 2009-10-07 | 天津市先石光学技术有限公司 | Implanted in-vivo continuous blood sugar monitor |
| CN101642380A (en) * | 2009-09-17 | 2010-02-10 | 华东理工大学 | Direct implantation type microdialysis probe and preparation method thereof |
| CN102504111A (en) * | 2011-11-17 | 2012-06-20 | 中国科学院化学研究所 | Electrode surface modification material, preparation method thereof and application thereof |
| CN102525569A (en) * | 2011-12-28 | 2012-07-04 | 浙江省肿瘤医院 | Array type microdialysis probe |
| CN203365179U (en) * | 2013-06-09 | 2013-12-25 | 覃思 | Microdialysis sensor |
Non-Patent Citations (2)
| Title |
|---|
| 张建锋 等: "固定化联吡啶钌电化学发光结合微透析采样在线研究药物蛋白反应", 《分析化学研究报告》, vol. 40, no. 5, 31 May 2012 (2012-05-31), pages 730 - 734 * |
| 陈郁初: "介绍一种活体在位实时监测脑内化学物质变化的新方法", 《生命科学》, vol. 13, no. 4, 31 August 2001 (2001-08-31), pages 189 - 192 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103822944A (en) * | 2014-02-12 | 2014-05-28 | 绿谷（集团）有限公司 | Real-time on-line detection method and device of rapid biological signals |
| CN104383631A (en) * | 2014-11-27 | 2015-03-04 | 王俐帧 | Anesthetic needle with measuring device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101755202B (en) | Sensor and apparatus for analysing gases present in blood | |
| US11224367B2 (en) | Sensor module and method of using a sensor module | |
| CN1456888A (en) | Physiological sample collector and use method thereof | |
| JPH11503654A (en) | Apparatus and method for continuous monitoring of a physiological analyte of a subject | |
| US20150057513A1 (en) | Minimally Invasive Stress Sensors and Methods | |
| Ali et al. | Intracellular K $^+ $ Determination With a Potentiometric Microelectrode Based on ZnO Nanowires | |
| CN101929977A (en) | Enzyme bioelectrochemical sensing chip and preparation and using methods thereof | |
| Zhou et al. | Biomedical sensor, device and measurement systems | |
| Poscia et al. | A novel continuous subcutaneous lactate monitoring system | |
| CN103308349A (en) | Micro-dialysis sensor | |
| Downs et al. | Microneedle electrochemical aptamer-based sensing: Real-time small molecule measurements using sensor-embedded, commercially-available stainless steel microneedles | |
| CN109464146B (en) | Myoelectricity inspection needle with treatment effect | |
| CN203365179U (en) | Microdialysis sensor | |
| CN2890919Y (en) | Cylindrical body fluid glucose electrochemical sensor | |
| CN203324287U (en) | Combined microdialysis experiment system | |
| CN208447588U (en) | A kind of medicative myoelectricity inspection needle of tool | |
| US10330630B2 (en) | Reader device and method of signal amplification | |
| CN207202871U (en) | A kind of physiologic parameter analysis system continuously or discontinuously | |
| CN109030597A (en) | Hollow Nano needle-graphene composite material sensor and its application | |
| JP2010504819A (en) | System and method for continuous detection of an analyte in the bloodstream | |
| CN108324273B (en) | Myoelectricity inspection needle | |
| CN106645345A (en) | Flexible bioelectrode | |
| Hutter et al. | Point-of-care and self-testing for potassium: Recent advances | |
| CN204734491U (en) | What can be used for lasting blood sugar monitoring has pressure measurement of a wound arteriopuncture needle | |
| Zeng et al. | Simultaneous detection of norepinephrine and 5-hydroxytryptophan using poly-alizarin/multi-walled carbon nanotubes-graphene modified carbon fiber microelectrode array sensor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130918 |