CN103063834A - Method and system for analysis of immune quantitative chromatographic assay strip - Google Patents
Method and system for analysis of immune quantitative chromatographic assay strip Download PDFInfo
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- CN103063834A CN103063834A CN2012105845448A CN201210584544A CN103063834A CN 103063834 A CN103063834 A CN 103063834A CN 2012105845448 A CN2012105845448 A CN 2012105845448A CN 201210584544 A CN201210584544 A CN 201210584544A CN 103063834 A CN103063834 A CN 103063834A
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Abstract
The invention provides a method and a system for analysis of an immune quantitative chromatographic assay strip. The method comprises the following steps of carrying out full-course scanning of a test zone of an immune quantitative chromatographic assay strip to be tested, acquiring fluorescence signal intensity of all test points in the test zone of the immune quantitative chromatographic assay strip, determining fluorescence signal intensity of a test band, a quality control band and the background of the immune quantitative chromatographic assay strip according to the fluorescence signal intensity of all the test points in the test zone, and carrying out analysis of the immune quantitative chromatographic assay strip according to the fluorescence signal intensity of the test band, the quality control band and the background, and a concentration of a test solution. The method can analyze an immune quantitative chromatographic assay strip with a test solution and improve a test precision of an immune quantitative chromatographic assay strip.
Description
Technical field
The application relates to immune field, particularly a kind of analytical approach and system of immune quantitative chromatography strip.
Background technology
Modern immunological technique is along with the development of immunolabelling technique.Adopt fluorescein labelled antibody technology radiation isotope mark finally from nineteen forty-one coons etc., more finally enzyme mark technology and labelling techniques such as collaurum, quantum dot, magnetic nano particle, latex particle.These technology are widespread use in clinical immunology at present, and providing of immune diagnostic technique has been provided greatly.Have the in recent years widespread use in clinical diagnosis of immunity-chromatography test strip of the advantage such as quick, reliable, easy and simple to handle, and be extended to the fields such as food safety detection, environment measuring.
Immunochromatography technique is the reaction principle according to antigen-antibody, binding film separation principle and a kind of detection technique of developing.It is detected solution to be added drop-wise to detect on the strip, capillarity by film interacts antibody (antigen) fixing on detected material and the film, thereby produce special detection line, obtain the concentration value of described detected solution according to described detection line by calculating.
Immune quantitative chromatography strip is the qualitative strip of collaurum mostly, and it is to judge the negative or positive of testing result according to the depth of detection line colour developing.The inventor finds through research, also do not have a kind ofly effectively immune quantitative chromatography strip to be carried out analyzing detecting method, can improve the accuracy of detection of immune quantitative chromatography strip.
Summary of the invention
The application's technical matters to be solved provides a kind of analytical approach of immune quantitative chromatography strip, can have the immune quantitative chromatography strip of detected solution to analyze to dripping, and improves the accuracy of detection of immune quantitative chromatography strip.
In order to address the above problem, the application discloses a kind of analytical approach of immune quantitative chromatography strip, comprising:
Surveyed area to immune quantitative chromatography strip to be detected carries out whole process scanning;
Obtain the fluorescence signal intensity of all test points in the surveyed area of described immune quantitative chromatography strip to be detected;
Determine the fluorescence signal intensity of the background of the fluorescence signal intensity of fluorescence signal intensity, quality control band of the detection band in the described immune quantitative chromatography strip and described immune quantitative chromatography strip according to the fluorescence signal intensity of each test point in the described surveyed area;
Described immune quantitative chromatography strip is analyzed in conjunction with the concentration of detected solution according to the fluorescence signal intensity of described detection band, the fluorescence signal intensity of quality control band, the fluorescence signal intensity of background.
Above-mentioned method, preferred, the concentration of the fluorescence signal intensity of the fluorescence signal intensity of the described detection band of described foundation, the fluorescence signal intensity of quality control band, background and detected solution is analyzed described immune quantitative chromatography strip and is comprised:
Calculate the first difference of the fluorescence signal intensity of the fluorescence signal intensity of described detection band and described background;
Calculate the second difference of the fluorescence signal intensity of the fluorescence signal intensity of described quality control band and described background;
Calculate respectively the first quotient of described the first difference and described the second difference and the second quotient of described the second difference and described the first difference;
The concentration of described the first quotient or described the second quotient and detected solution is carried out the correlativity match, realize the analysis to described immune quantitative chromatography strip.
Above-mentioned method, preferred, the fluorescence signal intensity of all test points comprises in the described surveyed area that obtains described immune quantitative chromatography strip to be detected:
Use photoelectric detective circuit and detect light to described zone to be detected emission, described detection reflection of light degree is determined the fluorescence signal intensity of each test point in the surveyed area of described immune quantitative chromatography strip to be detected according to each test point in the described zone to be detected.
Above-mentioned method, preferably, determine that according to the fluorescence signal intensity of each test point in the described surveyed area fluorescence signal intensity of the background of the fluorescence signal intensity of fluorescence signal intensity, quality control band of the detection band in the described immune quantitative chromatography strip and described immune quantitative chromatography strip comprises:
Fluorescence signal intensity according to each test point in the described surveyed area carries out the zone division to described each test point, thereby determines the background of detection band, quality control band and test zone in the described test zone;
Respectively the fluorescence signal intensity of all test points in described detection band, quality control band and the test zone background is averaged, thereby obtain the fluorescence signal intensity of the background of the fluorescence signal intensity of fluorescence signal intensity, quality control band of the detection band in the described immune quantitative chromatography strip and described immune quantitative chromatography strip.
A kind of analytic system of immune quantitative chromatography strip comprises:
Scanning element is used for the surveyed area of immune quantitative chromatography strip to be detected is carried out whole process scanning;
Acquiring unit is for the fluorescence signal intensity of all test points of surveyed area of obtaining described immune quantitative chromatography strip to be detected;
Determining unit is used for determining according to the fluorescence signal intensity of described each test point of surveyed area the fluorescence signal intensity of the background of the fluorescence signal intensity of fluorescence signal intensity, quality control band of the detection band in the described immune quantitative chromatography strip and described immune quantitative chromatography strip;
Analytic unit is for described immune quantitative chromatography strip being analyzed in conjunction with the concentration of detected solution according to the fluorescence signal intensity of described detection band, the fluorescence signal intensity of quality control band, the fluorescence signal intensity of background.
Above-mentioned system, preferred, described analytic unit comprises:
The first computation subunit, be used for calculating the fluorescence signal intensity of described detection band and described background fluorescence signal intensity the first difference and calculate the fluorescence signal intensity of described quality control band and the second difference of the fluorescence signal intensity of described background;
The second computation subunit is used for calculating respectively the first quotient of described the first difference and described the second difference and the second quotient of described the second difference and described the first difference;
Analyze subelement, be used for the concentration of described the first quotient or described the second quotient and detected solution is carried out the correlativity match, realize the analysis to described immune quantitative chromatography strip.
The analytical approach of a kind of immune quantitative chromatography strip that the embodiment of the present application provides, by the instrument device strip detection window is carried out whole process scanning, read the signal intensity of each point of surveyed area, then according to the Changing Pattern of signal intensity, automatically detect the signal intensity of band (T), quality control band (C), background (B) in the identification strip, carry out the correlativity match according to the concentration of (T-B)/(C-B) or ratio (C-B)/(T-B) and analyte.Improved the accuracy of detection sensitivity, sensing range and the strip of strip by this computing method.Simultaneously also make detection curve become " straight line " by former " S " type curve, thereby reduced the difficulty of strip difference between batch control.
Description of drawings
In order to be illustrated more clearly in the technical scheme in the embodiment of the present application, the accompanying drawing of required use was done to introduce simply during the below will describe embodiment, apparently, accompanying drawing in the following describes only is some embodiment of the application, for those of ordinary skills, under the prerequisite of not paying creative work, can also obtain according to these accompanying drawings other accompanying drawing.
Fig. 1 is the process flow diagram of the analytical approach of a kind of immune quantitative chromatography strip of providing of the embodiment of the present application;
Fig. 2 is the another process flow diagram of the analytical approach of a kind of immune quantitative chromatography strip of providing of the embodiment of the present application;
Fig. 3 is a kind of strip fluorescent scanning figure that the embodiment of the present application provides;
Fig. 4 is that a kind of fluorescence that the embodiment of the present application provides compares synoptic diagram;
Fig. 5 is the structural representation of the analytic system of a kind of immune quantitative chromatography strip of providing of the embodiment of the present application;
Fig. 6 is the another structural representation of the analytic system of a kind of immune quantitative chromatography strip of providing of the embodiment of the present application.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present application, the technical scheme in the embodiment of the present application is clearly and completely described, obviously, described embodiment only is the application's part embodiment, rather than whole embodiment.Based on the embodiment among the application, those of ordinary skills are not making the every other embodiment that obtains under the creative work prerequisite, all belong to the scope of the application's protection.
The application can be used in numerous general or special purpose calculation element environment or the configuration.For example: personal computer, server computer, handheld device or portable set, plate equipment, multi-processor device, comprise distributed computing environment of above any device or equipment etc.
The inventor finds through research, existing analytical approach to immune quantitative chromatography strip adopts the ratio of detection line and nature controlling line intensity quantitatively to calculate mostly, do not consider the strip background signal interference and since the NC film when stroke detection line and nature controlling line to the damage of NC film.Thereby cause this computing method not exclusively identical with actual strip test case.Greatly improved the inaccuracy of testing result.Simultaneously because particle flows at film, and when detecting, the NC film is except existing fluorescence signal on T, the C line, equally also there is fluorescence signal at the remainder of strip.When the computing method that adopt T/C or C/T, in fact the fluorescence intensity level at T, C line has comprised the background fluorescence signal value.When the detection material of test low concentration, because the fluorescence intensity of T line is very low, almost to distinguish when little than background fluorescence signal value, background fluorescence intensity can the severe jamming test value, and when test concentrations was high, the background fluorescence signal value also can disturb nature controlling line.Thereby cause strip inaccurate when test concentrations is low or high, reduced the test specification of strip, the while is owing to the interference of background, the sensitivity of also greatly having lowered strip.
With reference to figure 1, show the process flow diagram of the analytical approach of a kind of immune quantitative chromatography of the application strip, comprising:
Step S101: the surveyed area to immune quantitative chromatography strip to be detected carries out whole process scanning;
Step S102: the fluorescence signal intensity that obtains all test points in the surveyed area of described immune quantitative chromatography strip to be detected;
Use photoelectric detective circuit in the embodiment of the present application and detect light to described zone to be detected emission, described detection reflection of light degree is determined the fluorescence signal intensity of each test point in the surveyed area of described immune quantitative chromatography strip to be detected according to each test point in the described zone to be detected.
Step S103: the fluorescence signal intensity of determining the background of the fluorescence signal intensity of fluorescence signal intensity, quality control band of the detection band in the described immune quantitative chromatography strip and described immune quantitative chromatography strip according to the fluorescence signal intensity of each test point in the described surveyed area;
Fluorescence signal intensity according to each test point in the described surveyed area in the embodiment of the present application carries out the zone division to described each test point, thereby determines the background of detection band, quality control band and test zone in the described test zone;
Respectively the fluorescence signal intensity of all test points in described detection band, quality control band and the test zone background is averaged, thereby obtain the fluorescence signal intensity of the background of the fluorescence signal intensity of fluorescence signal intensity, quality control band of the detection band in the described immune quantitative chromatography strip and described immune quantitative chromatography strip.
Step S104: in conjunction with the concentration of detected solution described immune quantitative chromatography strip is analyzed according to the fluorescence signal intensity of described detection band, the fluorescence signal intensity of quality control band, the fluorescence signal intensity of background.
With reference to figure 2, show the another process flow diagram of the analytical approach of a kind of immune quantitative chromatography of the application strip, the concentration of the fluorescence signal intensity of the fluorescence signal intensity of the described detection band of described foundation, the fluorescence signal intensity of quality control band, background and detected solution is analyzed described immune quantitative chromatography strip and is comprised:
Step S201: the first difference of calculating the fluorescence signal intensity of the fluorescence signal intensity of described detection band and described background; And the second difference of the fluorescence signal intensity of the fluorescence signal intensity of the described quality control band of calculating and described background;
Step S202: calculate respectively the first quotient of described the first difference and described the second difference and the second quotient of described the second difference and described the first difference;
Step S203: the concentration of described the first quotient or described the second quotient and detected solution is carried out the correlativity match, realize the analysis to described immune quantitative chromatography strip.
For the more detailed analytical approach that the application is provided is described, the application provides following specific embodiment, the microdose urine protein strip is inserted in the mobile draw-in groove of immunofluorescence analysis instrument, analyser can begin to scan the fluorescent value of strip coding and strip automatically, after analyser confirms that strip does not have quality problems, mobile draw-in groove can withdraw from automatically, and prompting user can add the mixed solution of sample and fluorescent latex particles.When mixed liquor joins on the strip, instrument starts countdown function automatically, the automatic fluorescence signal intensity of reading test strips view window behind the 5min, and every strip instrument gathers 40 fluorescence signals automatically.Its collection result synoptic diagram such as the application are shown in Figure 3.
At first, be mixed with the microdose urine protein solution of variable concentrations, 300,250,200,150,100,75,50,25,10,5,1,0ug/ml concentration is respectively:, then the solution of getting the above-mentioned variable concentrations of 10ul joins in the fluorescent latex particles that 200ul contains antialbumin antibody fully mixing 1min, then get the 75ul mixed liquor and join in the strip, reading test strips fluorescent value behind the 5min.5 strips of each concentration determination, the average fluorescent strength value of 5 strips is as shown in table 1:
The average fluorescent strength value of table 15 strip
B1 is the average background fluorescent value of strip leading portion, the i.e. mean value of the 7th, 8,9 data.
B2 is the average background fluorescent value in strip stage casing, the i.e. mean value of the 20th, 21,22 data.
B3 is the average background fluorescent value of strip back segment, the i.e. mean value of the 37th, 38,39 data.
Above-mentioned original fluorescent value is processed, and its result synoptic diagram such as the application are shown in Figure 4.
Show the analytic system of a kind of immune quantitative chromatography of the application strip referring to Fig. 5, comprising:
Acquiring unit 302 is for the fluorescence signal intensity of all test points of surveyed area of obtaining described immune quantitative chromatography strip to be detected;
Determining unit 303 is used for determining according to the fluorescence signal intensity of described each test point of surveyed area the fluorescence signal intensity of the background of the fluorescence signal intensity of fluorescence signal intensity, quality control band of the detection band in the described immune quantitative chromatography strip and described immune quantitative chromatography strip;
Show a detailed construction synoptic diagram of the analytic system of a kind of immune quantitative chromatography of the application strip referring to Fig. 6, described analytic unit 304 comprises:
The first computation subunit 305, be used for calculating the fluorescence signal intensity of described detection band and described background fluorescence signal intensity the first difference and calculate the fluorescence signal intensity of described quality control band and the second difference of the fluorescence signal intensity of described background;
The second computation subunit 306 is used for calculating respectively the first quotient of described the first difference and described the second difference and the second quotient of described the second difference and described the first difference;
Analyze subelement 307, be used for the concentration of described the first quotient or described the second quotient and detected solution is carried out the correlativity match, realize the analysis to described immune quantitative chromatography strip.
Need to prove that each embodiment in this instructions all adopts the mode of going forward one by one to describe, what each embodiment stressed is and the difference of other embodiment that identical similar part is mutually referring to getting final product between each embodiment.For device class embodiment because itself and embodiment of the method basic simlarity, so describe fairly simple, relevant part gets final product referring to the part explanation of embodiment of the method.
More than analytical approach and the system of a kind of immune quantitative chromatography strip that the application is provided be described in detail, used specific case herein the application's principle and embodiment are set forth, the explanation of above embodiment just is used for helping to understand the application's method and core concept thereof; Simultaneously, for one of ordinary skill in the art, the thought according to the application all will change in specific embodiments and applications, and in sum, this description should not be construed as the restriction to the application.
Claims (6)
1. the analytical approach of an immune quantitative chromatography strip is characterized in that, comprising:
Surveyed area to immune quantitative chromatography strip to be detected carries out whole process scanning;
Obtain the fluorescence signal intensity of all test points in the surveyed area of described immune quantitative chromatography strip to be detected;
Determine the fluorescence signal intensity of the background of the fluorescence signal intensity of fluorescence signal intensity, quality control band of the detection band in the described immune quantitative chromatography strip and described immune quantitative chromatography strip according to the fluorescence signal intensity of each test point in the described surveyed area;
Described immune quantitative chromatography strip is analyzed in conjunction with the concentration of detected solution according to the fluorescence signal intensity of described detection band, the fluorescence signal intensity of quality control band, the fluorescence signal intensity of background.
2. method according to claim 1, it is characterized in that the concentration of the fluorescence signal intensity of the fluorescence signal intensity of the described detection band of described foundation, the fluorescence signal intensity of quality control band, background and detected solution is analyzed described immune quantitative chromatography strip and comprised:
Calculate the first difference of the fluorescence signal intensity of the fluorescence signal intensity of described detection band and described background; And the second difference of the fluorescence signal intensity of the fluorescence signal intensity of the described quality control band of calculating and described background;
Calculate respectively the first quotient of described the first difference and described the second difference and the second quotient of described the second difference and described the first difference;
The concentration of described the first quotient or described the second quotient and detected solution is carried out the correlativity match, realize the analysis to described immune quantitative chromatography strip.
3. method according to claim 1 is characterized in that, the fluorescence signal intensity of all test points comprises in the described surveyed area that obtains described immune quantitative chromatography strip to be detected:
Use photoelectric detective circuit and detect light to described zone to be detected emission, described detection reflection of light degree is determined the fluorescence signal intensity of each test point in the surveyed area of described immune quantitative chromatography strip to be detected according to each test point in the described zone to be detected.
4. according to claim 1 or 3 described methods, it is characterized in that, determine that according to the fluorescence signal intensity of each test point in the described surveyed area fluorescence signal intensity of the background of the fluorescence signal intensity of fluorescence signal intensity, quality control band of the detection band in the described immune quantitative chromatography strip and described immune quantitative chromatography strip comprises:
Fluorescence signal intensity according to each test point in the described surveyed area carries out the zone division to described each test point, thereby determines the background of detection band, quality control band and test zone in the described test zone;
Respectively the fluorescence signal intensity of all test points in described detection band, quality control band and the test zone background is averaged, thereby obtain the fluorescence signal intensity of the background of the fluorescence signal intensity of fluorescence signal intensity, quality control band of the detection band in the described immune quantitative chromatography strip and described immune quantitative chromatography strip.
5. the analytic system of an immune quantitative chromatography strip is characterized in that, comprising:
Scanning element is used for the surveyed area of immune quantitative chromatography strip to be detected is carried out whole process scanning;
Acquiring unit is for the fluorescence signal intensity of all test points of surveyed area of obtaining described immune quantitative chromatography strip to be detected;
Determining unit is used for determining according to the fluorescence signal intensity of described each test point of surveyed area the fluorescence signal intensity of the background of the fluorescence signal intensity of fluorescence signal intensity, quality control band of the detection band in the described immune quantitative chromatography strip and described immune quantitative chromatography strip;
Analytic unit is for described immune quantitative chromatography strip being analyzed in conjunction with the concentration of detected solution according to the fluorescence signal intensity of described detection band, the fluorescence signal intensity of quality control band, the fluorescence signal intensity of background.
6. system according to claim 5 is characterized in that, described analytic unit comprises:
The first computation subunit, be used for calculating the fluorescence signal intensity of described detection band and described background fluorescence signal intensity the first difference and calculate the fluorescence signal intensity of described quality control band and the second difference of the fluorescence signal intensity of described background;
The second computation subunit is used for calculating respectively the first quotient of described the first difference and described the second difference and the second quotient of described the second difference and described the first difference;
Analyze subelement, be used for the concentration of described the first quotient or described the second quotient and detected solution is carried out the correlativity match, realize the analysis to described immune quantitative chromatography strip.
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| CN104502590A (en) * | 2014-12-23 | 2015-04-08 | 北京出入境检验检疫局检验检疫技术中心 | Hepatitis A virus chromatography test paper strip based on low-noise excitation fluorescent label |
| CN104714009A (en) * | 2015-03-17 | 2015-06-17 | 深圳市海王英特龙生物技术股份有限公司 | Immunoassay detection system and detection method |
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| CN113009150A (en) * | 2021-02-20 | 2021-06-22 | 北京华科泰生物技术股份有限公司 | Concentration device for collecting urine microalbumin in sweat, detection kit comprising same and application thereof |
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