CN102928397A - Optical system of holographic needlepoint enhanced Raman spectrometer - Google Patents
Optical system of holographic needlepoint enhanced Raman spectrometer Download PDFInfo
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- CN102928397A CN102928397A CN2012104397887A CN201210439788A CN102928397A CN 102928397 A CN102928397 A CN 102928397A CN 2012104397887 A CN2012104397887 A CN 2012104397887A CN 201210439788 A CN201210439788 A CN 201210439788A CN 102928397 A CN102928397 A CN 102928397A
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Abstract
The invention provides an optical system of a holographic needlepoint enhanced Raman spectrometer and relates to the Raman spectrometer. The optical system comprises a laser, a beam splitter prism group, an edge filter, a lens with a high numerical aperture (NA) value, a sample objective table and a Raman signal acquisition lens, the beam splitter prism group is used for dividing a laser light beam sent out by the laser into two parallelly operating laser light beams, the beam splitter prism group comprises a block prism and a triangular prism, the edge filter is arranged at the front of the beam splitter prism group and enables the two laser light beams to enter the Raman signal acquisition lens in incident mode at the angle of 45 degrees, the lens with the high NA value is used for converging the two laser light beams reflected by the edge filter at a focus point of the tail end of a needlepoint, and the Raman signal acquisition lens is used for conveying acquired Raman signals to the spectrometer and a detector to perform light splitting and detection. The optical system is simple and convenient to operate. In practical application, upright and inverted modes can be designed aiming at different detection objects.
Description
Technical field
The present invention relates to Raman spectrometer, especially relate to holographic Tip-Enhanced Raman Spectroscopy instrument optical system.
Background technology
No matter conventional Raman spectrum or Surface enhanced raman spectroscopy, its spatial resolution all can't break through optical diffraction limit, thereby there is great limitation in the object of research.
It is found that in recent years, adopt " Tip-Enhanced Raman Spectroscopy technology " can reach tens nanometers to spatial resolution and the monomolecular detection sensitivity of the breakthrough optical diffraction limit of several Nano grades.Under normal pressure and air conditions, Tip-Enhanced Raman Spectroscopy technology (hereinafter referred to as TERS) be at present unique can be in the surface and interface optical characterisation technology of several nanometers to tens nanoscale sampling chemical analysis information.Therefore, no matter commercial Application, or basic scientific research all has very large demand to " Tip-Enhanced Raman Spectroscopy instrument ", is the study hotspot in present Raman spectrum field to development, the Application and Development of TERS instrument.Optical system is that the main of TERS also is important ingredient.
The basic functional principle of TERS is: utilize scanning probe microscopy that gold or silver-colored needle point are approached substrate, under the Ear Mucosa Treated by He Ne Laser Irradiation of suitable wavelength and polarization, tip end is because surface plasmon resonance effect produces the electromagnetic field that strengthens, and the Raman signal that therefore is positioned at needle point below sample is enhanced.TERS has high sensitivity and spatial resolution, and can obtain simultaneously pattern and the chemical information of sample surfaces.Only need adopt the laser power of milliwatt level to get final product, therefore be paid attention to widely.
At present, more international renowned companies, such as NTMDT, Renishaw and Nanonics have released business-like TERS instrument.The independent intellectual property rightization of TERS instrument also is seen in application, such as US Patent No. 2002/0154301, patent US2010/0245816; The acquired Chinese patent 201110354369.9 of Chinese patent CN101082585A and applicant.
A common feature of above TERS instrument is that its optical system substantially all is to be based upon on the basis of the burnt microtechnic of copolymerization.This is so that the instrumentation difficulty is large, and cost is high.The high-caliber research work of delivering in the world up to now substantially all is that the instrument of developing voluntarily in the laboratory obtains, and rarely has at commercial TERS instrument to obtain high-caliber achievement in research.
Summary of the invention
The object of the present invention is to provide holographic Tip-Enhanced Raman Spectroscopy instrument optical system.
The present invention is provided with:
A laser instrument, described laser instrument is used for the Raman signal of excited sample, as system's excitation source;
An Amici prism group, the beam of laser bundle that described Amici prism group is used for laser instrument is sent is divided into the excitation beam that two bundles run parallel, and described Amici prism group is provided with block prism and triangular prism, and described Amici prism is mounted on laser instrument the place ahead;
An edge filter sheet, the excitation beam that two bundles that described edge filter sheet forms for the reflection beam splitting prism run parallel, and on the Raman collection light path the exciting and the noises such as sample light, Rayleigh scattering of filtering reflection, described edge filter sheet is located at Amici prism group the place ahead, two bundle laser is incident in Raman signal with 45° angle gathers lens;
High NA value lens, described high NA value lens are used for two bundle excitation beams of edge filter sheet reflection are converged at a tip end focus point;
A sample stage, described sample stage are used for placing testing sample;
A Raman signal gathers lens, and the Raman signal that described Raman signal collection lens are used for gathering is transported to spectrometer and detecting device carries out light splitting and detection.
Described laser instrument can adopt LD laser instrument or gas laser etc.
Described edge filter sheet can adopt long pass filter sheet or notch filter sheet etc.
The present invention has following outstanding advantages:
1, the emitting laser bundle light beam that becomes two bundles to run parallel by the prism component, two light beams are realized assembling through the high-NA camera lens.Obviously, this is a kind of new holographic register system.This system is simple, easy to operate.In actual applications, can design for different detected objects and just putting and be inverted two kinds of patterns.
2, twin-beam excites when having avoided needle point and spot coupling owing to the reflection of needle tip bracket (for example semi-girder of afm tip) to exciting light Gauss center, greatly improved the launching efficiency of laser, and reduce the ground unrest that thus reflection causes, Raman spectrum is collected and signal is processed thereby effectively improve.
3, compact optical system is conducive to TERS instrument miniaturization and practical.Above characteristics make the present invention and patent US2002/0154301, and US2010/0245816, CN101082585A compare has unique superior and innovation.
4, the present invention is different from the product of having reported at present, and the present invention adopts the twin-beam focusing system, so claim holographic TERS optical system.With respect to the TERS instrument system of having reported at present convenient easy-to-use, can in actual production, research, obtain important application.
Description of drawings
Fig. 1 is the structural representation of positively fixed type optical system of the present invention.
Fig. 2 is the structural representation of inversion type optical system of the present invention.
In Fig. 1 and 2, respectively be labeled as: the 1st, laser instrument, the 2nd, block prism, the 3rd, triangular prism, the 4th, edge filter sheet, the 5th, high NA value lens, the 6th, needle point, the 7th, sample stage, the 8th, Raman signal gathers lens, and the 9th, raman spectroscopic system, the 10th, focused light is at the enlarged drawing of tip end.
Embodiment
Referring to Fig. 1 and 2, Fig. 1 provides the structural representation of positively fixed type optical system of the present invention, and Fig. 2 provides the structural representation of inversion type optical system of the present invention.
The embodiment of the invention is provided with:
A laser instrument 1, described laser instrument 1 is used for the Raman signal of excited sample, as system's excitation source;
An Amici prism group, the beam of laser bundle that described Amici prism group is used for laser instrument is sent is divided into the excitation beam that two bundles run parallel, and described Amici prism group is provided with block prism 2 and triangular prism 3, and described Amici prism is mounted on laser instrument 1 the place ahead;
An edge filter sheet 4, the excitation beam that two bundles that described edge filter sheet 4 is used for reflection beam splitting prism composition run parallel, and on the Raman collection light path the exciting and the noises such as sample light, Rayleigh scattering of filtering reflection, described edge filter sheet 4 is located at Amici prism group the place ahead, two bundle laser is incident in Raman signal with 45° angle gathers lens 8;
High NA value lens 5, described high NA value lens 5 are used for two bundle excitation beams of edge filter sheet 4 reflections are converged at a needle point 6 terminal focus points;
A sample stage 7, described sample stage 7 is used for placing testing sample;
Raman signal gathers lens 8, and described Raman signal gathers Raman signal that lens 8 are used for gathering and is transported to raman spectroscopic system 9(and comprises spectrometer and detecting device) carry out light splitting and detection.
Described laser instrument 1 can adopt LD laser instrument or gas laser etc.
Described edge filter sheet 4 can adopt long pass filter sheet or notch filter sheet etc.
In Fig. 1, adopt the positively fixed type structure, namely testing sample is opaque, and needle point 6 inserts from the two light beams angle, and light beam can drop on needle point 6 ends by focus point.
In Fig. 2, adopt the inversion type structure, namely testing sample is the transparent body, needle point 6 ends are positioned at twin-beam can focus point.
Claims (3)
1. holographic Tip-Enhanced Raman Spectroscopy instrument optical system is characterized in that being provided with:
A laser instrument, described laser instrument is used for the Raman signal of excited sample, as system's excitation source;
An Amici prism group, the beam of laser bundle that described Amici prism group is used for laser instrument is sent is divided into the excitation beam that two bundles run parallel, and described Amici prism group is provided with block prism and triangular prism, and described Amici prism is mounted on laser instrument the place ahead;
An edge filter sheet, the excitation beam that two bundles that described edge filter sheet forms for the reflection beam splitting prism run parallel, and on the Raman collection light path the exciting and the noises such as sample light, Rayleigh scattering of filtering reflection, described edge filter sheet is located at Amici prism group the place ahead, two bundle laser is incident in Raman signal with 45° angle gathers lens;
High NA value lens, described high NA value lens are used for two bundle excitation beams of edge filter sheet reflection are converged at a tip end focus point;
A sample stage, described sample stage are used for placing testing sample;
A Raman signal gathers lens, and the Raman signal that described Raman signal collection lens are used for gathering is transported to spectrometer and detecting device carries out light splitting and detection.
2. holographic Tip-Enhanced Raman Spectroscopy instrument optical system as claimed in claim 1 is characterized in that described laser instrument adopts LD laser instrument or gas laser.
3. holographic Tip-Enhanced Raman Spectroscopy instrument optical system as claimed in claim 1 is characterized in that described edge filter sheet adopts long pass filter sheet or notch filter sheet.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103743720A (en) * | 2014-01-20 | 2014-04-23 | 厦门大学 | Confocal microscopic Raman spectrometer with angle resolution capacity |
| CN103743721A (en) * | 2014-01-20 | 2014-04-23 | 厦门大学 | Plasmon-enhanced Raman spectroscopy dynamic detection system |
| CN103852461A (en) * | 2014-03-28 | 2014-06-11 | 厦门大学 | Electrochemical needle point enhanced Raman spectrometry instrument based on scanning probe microscope |
| CN105067587A (en) * | 2015-08-17 | 2015-11-18 | 苏州优谱德精密仪器科技有限公司 | Enhanced Raman spectroscopy system |
| CN105067588A (en) * | 2015-08-17 | 2015-11-18 | 苏州优谱德精密仪器科技有限公司 | Novel enhanced Raman spectroscopy system |
| CN105510296A (en) * | 2015-12-29 | 2016-04-20 | 北京华泰诺安探测技术有限公司 | Portable fluorescence-disappearance Raman spectrum detection system |
| CN106323470A (en) * | 2016-08-04 | 2017-01-11 | 北京华泰诺安探测技术有限公司 | Polarization modulation Raman probe using spatial output Laser, and spectral detection method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103743720A (en) * | 2014-01-20 | 2014-04-23 | 厦门大学 | Confocal microscopic Raman spectrometer with angle resolution capacity |
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| CN105510296A (en) * | 2015-12-29 | 2016-04-20 | 北京华泰诺安探测技术有限公司 | Portable fluorescence-disappearance Raman spectrum detection system |
| CN105510296B (en) * | 2015-12-29 | 2018-08-31 | 北京华泰诺安探测技术有限公司 | The portable fluorescence Raman spectrum detection system that disappears |
| CN106323470A (en) * | 2016-08-04 | 2017-01-11 | 北京华泰诺安探测技术有限公司 | Polarization modulation Raman probe using spatial output Laser, and spectral detection method |
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