CN102835962A - Collecting box for collecting cord blood and use method of collecting box - Google Patents
Collecting box for collecting cord blood and use method of collecting box Download PDFInfo
- Publication number
- CN102835962A CN102835962A CN2012103191092A CN201210319109A CN102835962A CN 102835962 A CN102835962 A CN 102835962A CN 2012103191092 A CN2012103191092 A CN 2012103191092A CN 201210319109 A CN201210319109 A CN 201210319109A CN 102835962 A CN102835962 A CN 102835962A
- Authority
- CN
- China
- Prior art keywords
- blood
- collecting
- cord blood
- anticoagulant
- collection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention discloses a collecting box for collecting cord blood and a use method of the collecting box. The collecting box comprises a collecting box, wherein a container for aseptically collecting cord blood is arranged in the box; and a sufficient non-liquid anticoagulant is filled in the container for collecting cord blood, and more specifically, the container for collecting cord blood is an injector or a collecting bag; the non-liquid anticoagulant comprises heparin, heparinoid type additives, citrate, ethylene diamine tetraacetic acid and oxalic acid. The invention also provides the use method of the collecting box for collecting cord blood. The use method comprises the following steps of: a, checking and disinfecting; b, checking the container; c, starting to collect; d, collecting a blood sample; e, repeatedly collecting; and f, mixing. All appliances and parts of the collecting box can be produced in batch, assembled and disinfected; the collecting box is good in stability, convenient to use and low in cost, and a human error of quantitative use of the anticoagulant can be eliminated; and meanwhile, the use method provided by the invention is simple to operate and a large amount of training time is saved.
Description
Technical field
The present invention relates to be used to collect the collection box and the method for using thereof of Cord blood.
Background technology
Cord blood is the blood that remains in after neonatal umbilical cord is cut off in umbilical cord or the placenta tissue.Generally speaking, the umbilical cord behind the baby due is dropped as biological waste with Placenta Hominis.Cord blood contains the high stem cell of medical value, like hematopoietic stem cell, can be used for many different stem cell transplantations and regenerative medicine and uses, such as the HSCT to hemocyte deficiency disorders and tumor disease therapeutic.Therefore,, collect and preserve these stem cell, be clinical practice in the future, necessary guarantee is provided as the infant umbilical cord blood in stem cell and other monocytic important source.
Human hematopoietic stem is the blast cell of blood various types of cells.Erythrocyte, leukocyte, platelet etc. all are by differentiation of stem cells.Therefore, they are fundamentals of setting up human immune system and blood system.The source of hematopoietic stem cell has three kinds: bone marrow, peripheral blood and Cord blood.Cord blood stem cell is because have the low characteristics weak with immunogenicity of himself differentiation degree, and it has the purposes of uniqueness.In the cell transplantation operation, because its immunogenicity is low, umbilical cord blood transplantation can reduce the immunological rejection of body.In addition, in stem cell transplantation, the blood system of human body even if single stem cell can regenerate.Therefore, it is vital for the weak immune system reconstruction of patient.Except the stem cell transplantation of hemopoietic system.Cord blood can also be divided into, and is used to treat heart disease, blood vessel, apoplexy, and the cell of commonly encountered diseases such as senile dementia has brought hope for curing these diseases.
The purposes of cord blood stem cell is a lot, but cell quantity of transplanting and transplant time are very important for therapeutic effect, so should maximized collection and storage cord blood stem cell.Yet the acquisition method of Cord blood but is not an ideal very at present.
Usually when collecting Cord blood, the collection personnel can use the container that contains the traditional liquid anticoagulant to prevent blood clotting, such as the collection container that contains liquid heparin or liquid sodium citrate.Yet before blood collecting, doctor or nurse are expelled to the liquid anticoagulant in the collection container earlier in operating room or delivery room meeting.Prepackage here has the non-sterile collection bag of CPD anticoagulant also often to be used, and when carrying out blood collection, is that strict aseptic requirement is arranged to these collecting bags.Catcher needs aseptic sterilization before use, but disinfecting process can cause destruction to a certain degree by the liquid towards anticoagulant.On the other hand; The anticoagulant interpolation is carried out in manual work can bring certain pollution risk; And the environment because of the delivery room is not always very to clean when the practical operation of hospital; Such as, thereby umbilical cord is in the environment of blood that has mother or urine and pollutes, so the collection that will adopt convenient feasible sterile equipment in advance, material and sterile working's method to carry out Cord blood is essential.In addition, anticoagulant adds excessive or low excessively or the inefficacy of anticoagulant own all can cause blood clotting, thereby reduces the practical value of Cord blood.Experiment is proof further, uses liquid anticoagulant, presses because change Premeabilisation of cells, also can cause the cord blood cells response rate to reduce.
The collection personnel need feasible sterile equipment easy to use, material and acquisition method; Under aseptic or half gnotobasis, carry out cord blood collection; Disinfect or be inappropriate under the environment of repetitive operation thereby completely cut off, the artificial risk of adding or using liquid anticoagulant to bring because of not passing through.
Summary of the invention
The technical problem that the present invention will solve is to overcome above-mentioned defective, provides a kind of simple in structure, easy and simple to handle and guarantee the collection box and the method for using thereof that are used to collect Cord blood of Cord blood collecting effect.
For addressing the above problem, the technical scheme that the present invention adopted is:
Be used to collect the collection box of Cord blood, it is characterized in that: comprise that is collected a box, be provided with aseptic collection Cord blood container in the box, collect the on-liquid anticoagulant that is provided with capacity in the Cord blood container.
Say further:
Said collection Cord blood container is syringe or collection bag.
Said on-liquid anticoagulant comprises heparin, heparinoid type additive, citrate, ethylenediaminetetraacetic acid and oxalic acid.
Said on-liquid anticoagulant is a freeze-dried type, or is Powdered.
Further say:
Also be provided with wherein one or several of aseptic cleaning wiping cloth, syringe, pallet, identification label, operation instruction, gauze, needle holder in the said aseptic box.
The present invention also provides a kind of method for using that is used to collect the collection box of Cord blood, it is characterized in that:
Comprise following steps:
A, inspection sterilization: check the parts intact condition in the aseptic box; Whether the inspection syringe has seal cover, device body free from flaw, and syringe does not lack parts and imperfect;
B, syringe should install non-liquid anticoagulant, guarantee that its dosage should be sufficient, are enough to prevent that the Cord blood of collecting from condensing;
C, begin to collect, clamp umbilical cord, and cut off in locating near the baby;
D, with after speckling with around the gauze wiping umbilical cord on position of disinfectant solution, use the fetus end blood sample collection of syringe from umbilical vein;
E, repetition d step reach the purpose of collecting maximum; And every syringe all should insert from umbilical cord at a distance;
F, collected umbilical blood with syringe after, move around with rotation mode and to mix the blood of anticoagulant and collection, so that make the effective mixing of anticoagulant and blood reach the anticoagulant effect.
The present invention also provides the another kind of method for using that is used to collect the collection box of Cord blood, it is characterized in that:
Comprise following steps:
The method for using of collecting bag:
A, inspection sterilization: check the apparatus status in the aseptic box; Whether the conduit of inspection collection bag bending occurs, or tearing appears in sack and conduit or the situation of cut, and whether inspection has the parts and the capping of disappearance;
B, collection bag should install non-liquid anticoagulant, guarantee that its dosage should be sufficient, are enough to prevent that the Cord blood of collecting from condensing;
C, begin to collect, clamp umbilical cord, and cut off in locating near the baby;
D, with after speckling with around the gauze wiping umbilical cord on position of disinfectant solution, use needle tubing on the collection bag from the fetus end blood sample collection of umbilical vein; For making flow maximization, cause the suitable lower position, needle tubing insert division that maintains of collection bag of discharging by gravity;
E, collected umbilical blood with collecting bag after, move around with rotation mode and to mix the blood of anticoagulant and collection, so that make the effective mixing of anticoagulant and blood reach the anticoagulant effect; After blood flow stops, preferably squeezing the remaining blood volume of umbilical cord and get in the pipe, so that collect more umbilical cord blood volume.
Adopted above technical scheme, each device component of the collection box among the present invention can be produced by batch, the sterilization of assembling back, and its product stability is good, and convenient the use is cheap, and can stop the human error that anticoagulant quantitatively uses.In the prepackage anticoagulant, the operator can avoid forgetting the probability of adding anticoagulant.Use freeze-dried type or other non-liquid anticoagulant also can improve the response rate of handling the Cord blood sample.Opposite with liquid, thus the osmotic pressure that uses the umbilical cord blood sample of non-liquid anticoagulant can not change the hemocyte film produces bad result.In addition, the use of non-liquid anticoagulant can promote the uniform distribution of anticoagulant in the Cord blood sample.Another advantage of non-liquid anticoagulant is that storage temperature is adaptive extensively.The picture some liquid anticoagulant, especially heparin, its storage temperature with 25 the degree be advisable, by contrast, the storage temperature of the non-liquid heparin among the present invention can be from 0 the degree about to 30 degree left and right sides scopes.Therefore, this invention can be guaranteed the stability of anticoagulant, reduces the osmotic pressure of cell membrane, increases substantially the cell response rate, can also improve the sample collection amount.Method for using provided by the invention, simple to operate, common medical personnel do training slightly and can skillfully operate, and have practiced thrift a large amount of training times.
The specific embodiment
Embodiment:
Be used to collect the collection box of Cord blood, comprise that is collected a box, be provided with in the box and collect the Cord blood sterile chamber, collect the on-liquid anticoagulant that is provided with capacity in the Cord blood container.
In the present embodiment, said collection Cord blood container is syringe or collection bag.Syringe or collection bag are two kinds of different acquisition instruments, can provide two kinds of methods for using that acquisition instrument is different respectively below.
In order to guarantee the anticoagulant effect, said on-liquid anticoagulant comprises heparin, heparinoid type additive, citrate, ethylenediaminetetraacetic acid and oxalic acid, and the on-liquid anticoagulant is a freeze-dried type, or is Powdered.
In addition, also be provided with wherein one or several of aseptic cleaning wiping cloth, syringe, pallet, identification label, operation instruction, gauze, needle holder in the said aseptic box.
Each device component of collection box among the present invention can be produced by batch, the sterilization of assembling back, and its product stability is good, and convenient the use is cheap, and can stop the human error that anticoagulant quantitatively uses.In the prepackage anticoagulant, the operator can avoid forgetting the probability of adding anticoagulant.Use freeze-dried type or other non-liquid anticoagulant also can improve the response rate of handling the Cord blood sample.Opposite with liquid, thereby can not changing the hemocyte osmotic pressure, the umbilical cord blood sample that adds non-liquid anticoagulant do not produce bad result.In addition, the use of non-liquid anticoagulant can promote the uniform distribution of anticoagulant in the Cord blood sample.Another advantage of non-liquid anticoagulant is the extensive of storage temperature.The picture some liquid anticoagulant, especially heparin, its storage temperature with 25 the degree be advisable, by contrast, the storage temperature of the non-liquid heparin among the present invention can be from 0 the degree about to 30 degree left and right sides scopes.Therefore, this invention not only can improve cell yield and can also improve the sample collection amount.
In the present embodiment, the syringe when acquisition instrument is syringe is collected the method for using of box, comprises following steps:
A, inspection sterilization: check the apparatus status in the aseptic box; Whether the inspection syringe has seal cover, device body free from flaw, and syringe does not lack parts and imperfect;
B, syringe should install non-liquid anticoagulant, guarantee that its dosage should be sufficient, are enough to prevent that the Cord blood of collecting from condensing;
C, begin to collect, clamp umbilical cord, and cut off in locating near the baby;
D, with after speckling with around the gauze wiping umbilical cord on position of disinfectant solution, use the fetus end blood sample collection of syringe from umbilical vein;
E, repetition d step reach the purpose of collecting maximum; And every syringe all should insert from umbilical cord at a distance;
F, collected umbilical blood with syringe after, move around with rotation mode and to mix the blood of anticoagulant and collection, so that make the effective mixing of anticoagulant and blood reach the anticoagulant effect.
In the present embodiment, the collection bag when acquisition instrument is collection bag is collected the method for using of box, comprises following steps:
A, inspection sterilization: check the apparatus status in the aseptic box; Whether the conduit of inspection collection bag bending occurs, or tearing appears in sack and conduit or the situation of cut, and whether inspection has the parts and the capping of disappearance;
B, collection bag should install non-liquid anticoagulant, guarantee that its dosage should be sufficient, are enough to prevent that the Cord blood of collecting from condensing;
C, begin to collect, clamp umbilical cord, and cut off in locating near the baby;
D, with after speckling with around the gauze wiping umbilical cord on position of disinfectant solution, use needle tubing on the collection bag from the fetus end blood sample collection of umbilical vein; For making flow maximization, cause the suitable lower position, needle tubing insert division that maintains of collection bag of discharging by gravity;
E, collected umbilical blood with collecting bag after, move around with rotation mode and to mix the blood of anticoagulant and collection, so that make the effective mixing of anticoagulant and blood reach the anticoagulant effect; After blood flow stops, preferably squeezing the remaining blood volume of umbilical cord and get in the pipe, so that collect more umbilical cord blood volume.
Two kinds of methods for using provided by the invention, simple to operate, common medical personnel do training slightly and can skillfully operate, and have practiced thrift a large amount of training times.
Above-described only is preferred implementation of the present invention, should be pointed out that for the person of ordinary skill of the art, under the prerequisite that does not break away from the invention design, can also make some distortion and improvement, and these all belong to protection scope of the present invention.
Claims (7)
1. be used to collect the collection box of Cord blood, it is characterized in that: comprise that is collected a box, be provided with aseptic collection Cord blood container in the box, collect the on-liquid anticoagulant that is provided with capacity in the Cord blood container.
2. collect the collection box of Cord blood according to being used to described in the claim 1, it is characterized in that: said collection Cord blood container is syringe or collection bag.
3. collect the collection box of Cord blood according to being used to described in the claim 2, it is characterized in that: said on-liquid anticoagulant comprises heparin, heparinoid type additive, citrate, ethylenediaminetetraacetic acid and oxalic acid.
4. collect the collection box of Cord blood according to being used to described in the claim 3, it is characterized in that: said on-liquid anticoagulant is a freeze-dried type, or is Powdered.
5. collect the collection box of Cord blood according to being used to described in the claim 1, it is characterized in that: also be provided with wherein one or several of aseptic cleaning wiping cloth, syringe, pallet, identification label, operation instruction, gauze, needle holder in the said box.
6. be used to collect the method for using that the syringe of Cord blood is collected box, it is characterized in that:
Comprise following steps:
A, inspection sterilization: the parts intact condition in the box is collected in inspection; Whether the inspection syringe has seal cover, device body free from flaw, and syringe does not lack parts and imperfect;
B, syringe should install non-liquid anticoagulant, guarantee that its dosage should be sufficient, are enough to prevent that the Cord blood of collecting from condensing;
C, begin to collect, clamp umbilical cord, and cut off in locating near the baby;
D, with after speckling with around the gauze wiping umbilical cord on position of disinfectant solution, use the fetus end blood sample collection of syringe from umbilical vein;
E, repetition d step reach the purpose of collecting maximum; And every syringe all should insert from umbilical cord at a distance;
F, collected umbilical blood with syringe after, move around with rotation mode and to mix the blood of anticoagulant and collection, so that make the effective mixing of anticoagulant and blood reach the anticoagulant effect.
7. be used to collect the method for using that the collection bag of Cord blood is collected box, it is characterized in that:
Comprise following steps:
The method for using of collecting bag:
A, inspection sterilization: check the parts intact condition in the aseptic box; Whether the conduit of inspection collection bag bending occurs, or tearing appears in sack and conduit or the situation of cut, and whether inspection has the parts and the capping of disappearance;
B, collection bag should install non-liquid anticoagulant, guarantee that its dosage should be sufficient, are enough to prevent that the Cord blood of collecting from condensing;
C, begin to collect, clamp umbilical cord, and cut off in locating near the baby;
D, with after speckling with around the gauze wiping umbilical cord on position of disinfectant solution, use needle tubing on the collection bag from the fetus end blood sample collection of umbilical vein; For making flow maximization, cause the suitable lower position, needle tubing insert division that maintains of collection bag of discharging by gravity;
E, collected umbilical blood with collecting bag after, move around with rotation mode and to mix the blood of anticoagulant and collection, so that make the effective mixing of anticoagulant and blood reach the anticoagulant effect; After blood flow stops, preferably squeezing the remaining blood volume of umbilical cord and get in the pipe, so that collect the Cord blood of maximum.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012103191092A CN102835962A (en) | 2012-08-31 | 2012-08-31 | Collecting box for collecting cord blood and use method of collecting box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012103191092A CN102835962A (en) | 2012-08-31 | 2012-08-31 | Collecting box for collecting cord blood and use method of collecting box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102835962A true CN102835962A (en) | 2012-12-26 |
Family
ID=47363887
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012103191092A Pending CN102835962A (en) | 2012-08-31 | 2012-08-31 | Collecting box for collecting cord blood and use method of collecting box |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102835962A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5484060A (en) * | 1995-02-09 | 1996-01-16 | Middle; George H. | Sterile infant identification device |
| US5674227A (en) * | 1996-05-08 | 1997-10-07 | Burns; Margaret K. | Afterbirth retaining device |
| CN1272355A (en) * | 2000-05-10 | 2000-11-08 | 朱璋根 | Disposable aseptic omphalotomy box |
| CN201127651Y (en) * | 2007-11-30 | 2008-10-08 | 任祥美 | Disposable article kit for sampling venous blood sample |
| US20090209031A1 (en) * | 2006-01-26 | 2009-08-20 | Tyco Healthcare Group Lp | Medical device package |
| CN102335046A (en) * | 2011-08-02 | 2012-02-01 | 江苏省北科生物科技有限公司 | Package box of cord blood collection |
-
2012
- 2012-08-31 CN CN2012103191092A patent/CN102835962A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5484060A (en) * | 1995-02-09 | 1996-01-16 | Middle; George H. | Sterile infant identification device |
| US5674227A (en) * | 1996-05-08 | 1997-10-07 | Burns; Margaret K. | Afterbirth retaining device |
| CN1272355A (en) * | 2000-05-10 | 2000-11-08 | 朱璋根 | Disposable aseptic omphalotomy box |
| US20090209031A1 (en) * | 2006-01-26 | 2009-08-20 | Tyco Healthcare Group Lp | Medical device package |
| CN201127651Y (en) * | 2007-11-30 | 2008-10-08 | 任祥美 | Disposable article kit for sampling venous blood sample |
| CN102335046A (en) * | 2011-08-02 | 2012-02-01 | 江苏省北科生物科技有限公司 | Package box of cord blood collection |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11571187B2 (en) | Method for obtaining sterile human amniotic fluid and uses thereof | |
| JP5229342B2 (en) | Serum preparation method | |
| AU2017283496B2 (en) | Modular device for cell spraying | |
| US20080287829A1 (en) | Collection means and a method for collecting cord blood | |
| JP5357139B2 (en) | Tissue collection | |
| CN204521969U (en) | Nursing decontaminating apparatus | |
| CN104152405B (en) | The method of separation and Extraction hematopoietic stem cell from Placenta Hominis | |
| CN206443861U (en) | A kind of medical tray with preliminarily pasteurized function | |
| WO2018062075A1 (en) | Tool for aseptically treating suspension | |
| CN107902213A (en) | A kind of clinical laboratory's sample storage device | |
| CN202128658U (en) | Medical nursing rescue carriage | |
| CN102835962A (en) | Collecting box for collecting cord blood and use method of collecting box | |
| CN201350187Y (en) | Portable checkbox | |
| CN204274894U (en) | The special ease of Use platform of nurse-in-charge | |
| CN204468297U (en) | Operation puncturing pin container | |
| CN113288662A (en) | Multifunctional movable operation nursing device convenient to clean | |
| CN203436685U (en) | Sterilizing eraser | |
| RU2523563C1 (en) | Bone marrow technology from dead donors with beating and non-beating heart | |
| CN216376000U (en) | Hospital infection is with medical waste collection device that can prevent leaking | |
| RU175546U1 (en) | DEVICE FOR PREPARING FILLING BODY FOR CLOSING THE BONE DEFECT | |
| CN209695392U (en) | A kind of packet for culture of drawing blood | |
| US20120004635A1 (en) | Method and means for collecting cord blood | |
| CN220175709U (en) | A keep ware for blood platelet rich plasma partial shipment | |
| CN211784541U (en) | Closed urine collecting and specimen collecting device | |
| CN211094745U (en) | Novel operating table is used in operating room nursing |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20121226 |