CN102680303A - Instrument for sectioning and dyeing biological sample on electronic microscope - Google Patents
Instrument for sectioning and dyeing biological sample on electronic microscope Download PDFInfo
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- CN102680303A CN102680303A CN2011100638019A CN201110063801A CN102680303A CN 102680303 A CN102680303 A CN 102680303A CN 2011100638019 A CN2011100638019 A CN 2011100638019A CN 201110063801 A CN201110063801 A CN 201110063801A CN 102680303 A CN102680303 A CN 102680303A
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- groove
- biological sample
- electron microscope
- turnover panel
- truss
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Abstract
The invention relates to an instrument for sectioning and dyeing a biological sample on an electronic microscope, which belongs to the field of biological sample preparation. The instrument is composed of a locating handle, a water outlet, a groove flap board, a wattle, a built-in coverless box, a top cover, a stable gasket and a groove frame, wherein the groove flap board is arranged in a top groove of the groove frame; the locating handle is arranged on the outer side of the groove frame; the wattle is arranged above the groove flap board; the built-in coverless box is located in the top groove of the groove frame and arranged on one side of the groove flap board; the top cover is covered on the top groove of the groove frame; and the bottom part of the groove frame is provided with the stable gasket. According to the invention, the instrument can perform dyeing on biological sample parity for many times without repeated manual operations; thus, the time difference can be reduced; the generation of pollutant is eliminated; a high-quality experiment result is ensured to obtain; and the instrument can be used for immunohistochemical incubation of a histochemical microscope and preparation of macromolecule sample drop tablets and the like.
Description
Technical field
The invention belongs to the biological sample preparation field, be specifically related to a kind of apparatus that is used for the section statining of electron microscope biological sample.
Background technology
Since electron microscope was born, people constantly explored and improve the technology of the preparation in early stage of the biological sample that is used for electron microscope observation, section, dyeing always, in the hope of obtaining more high-quality realistic images and experimental result.Colouring method after the conventional biolgoical sample embedded section adopts pure manual operations usually; Time difference when dyeing because of lead is uncontrollable; Precision is difficult to grasp; The deposition that plumbous dyeing liquor and carbon dioxide in air reaction cause can produce biological sample to be polluted, and causes obtaining high-quality experimental result; Also because the operation of original colouring method is more loaded down with trivial details; So regular meeting adopted piece to dye when biological sample was carried out (acetic acid uranium) dyeing; Prepare a routine sample and need use the above acetic acid uranium dye liquor of 1ml at least, thereby also correspondingly caused the waste and the pollution of acetic acid uranium solution.
Summary of the invention
The objective of the invention is to overcome the defective and the deficiency of prior art; A kind of apparatus that is used for the section statining of electron microscope biological sample is provided, the pollution that this apparatus can solve the loaded down with trivial details process of conventional biological sample electron microscope sample dyeing course and reduce acetic acid uranium use amount and reduce section statining.
The apparatus utilization location turnover panel that is used for the section statining of electron microscope biological sample of the present invention adds location snap close function technology; To repeatedly dye before the electron microscope sample observation (dyeing of acetic acid uranium, plumbous dyeing, negative staining); Need not to carry out repeatedly hand process, can reduce the mistiming and the generation of destroy contaminants.
Particularly; The apparatus that is used for the section statining of electron microscope biological sample of the present invention; It is characterized in that; Comprise that but snap close controls built-in turnover panel and secluding air flow-through grooves, by position fixing knob 1, water delivering orifice 2, groove turnover panel 3, lath 4, built-in uncovered box 5, top cover 6, stablize packing ring 7 and truss 8 is formed; Described groove turnover panel 3 places in the top groove of truss 8; Position fixing knob 1 is located at truss 8 outsides, its position is corresponding with groove turnover panel 3; Described lath 4 places the top of groove turnover panel 3; Described built-in uncovered box 5 is positioned at the top groove of truss 8, a side of groove turnover panel 3, and top cover 6 covers on the top groove of truss 8, and the bottom of truss 8 is provided with for four jiaos stablizes packing ring 7.
Among the present invention, described position fixing knob 1 is for turning to position fixing knob, the upset of control groove turnover panel 3, turns to, and its length is 4cm, handle be provided with can 30 ℃, 45 ℃ and 60 ℃ location locating button;
Described water delivering orifice 2 is used for getting rid of the solution of groove, which is provided with valve, can close water delivering orifice, and said diameter valve is 0.5cm;
Among the present invention, described groove turnover panel 3 places in the groove of truss 8, and this groove turnover panel 3 is rotatable, in one embodiment of the invention, the length of groove turnover panel 3 be 16cm, width be 0.6cm, highly for 0.3cm;
Among the present invention; Described lath 4 employing silica gel are processed, and which is provided with the band location and carry net (copper mesh, nickel screen etc.) embedding mouth, and this lath 4 is protruding embedded for down; In one embodiment of the invention; Its lower bottom part length is 16cm, the wide 0.6cm of being, the high 0.3cm of being, upper base length is that cm, height are the silica gel strip of 0.3cm (type paraffin plate function) for 16cm, wide 1.2, can set 16 on this lath and be colored year net of cutting into slices;
Among the present invention, described built-in uncovered box 5 is movable, and its length is that 3.5cm, width are 1.6cm, highly are to be used to insert sodium hydrate particle by 1.0cm;
Among the present invention, described top cover 6 is a button-type, and physical dimension is 22.4cm*2.2cm*0.8cm, and wherein, the following length of side of its button-type interface is 22.4cm, the wide 2.2cm of being, the high 0.3cm of being, the last length of side of button-type interface is 22cm, the wide 1.8cm of being, the high 0.5cm of being; The surface on described top 6 is provided with the handle 9 that physical dimension is 5cm*1.0cm*0.5cm;
Among the present invention, described stable packing ring 7 adopts rubber to process, and is provided with four usually;
Among the present invention, described truss 8 is a ladder-shaped frame, and its physical dimension is: long for 22cm, highly be 8cm, wherein, the seat end wide be 6cm, thickly be 0.5cm, the top of truss 8 is long be 22cm, wide be 1.8cm; The top of truss 8 is provided with groove; The length of this inside grooves is 20cm; Its left 16cm place is a boundary 10, and groove reprint 3 is established in boundary 10 left sides, built-in uncovered box 5 is established on the right side, and the height of boundary 10 is 1.8cm; The height in boundary 10 left sides is 2.3cm, width 1.8cm, and the height on boundary 10 right sides is that 1.8cm, width are 1.8cm.
During practice; The net (copper mesh, nickel screen etc.) that carries that only needs to have the biological sample section by the setting order embeds the embedding mouth location on the lath 4 with the angle of 45 or 90 degree; And with on the lath 4 placement groove turnover panels 3; Add the carbon dioxide that sodium hydrate particle is used to absorb local space for 5 li at built-in uncovered capsule, produce sediment to reduce the reaction of carbon dioxide and plumbous dyeing liquor; Top cover 6 was covered 1-2 minute; Taking down top cover 6 needs to carry net biologic slice one side having of dyeing at every, drips fast with pipettor and goes up dyeing liquor bonnet upper top cover 5-8 minute; Take down top cover 6, positioning of rotating handle 1 makes the location snap into miter angle, gets distilled water with garter drop method, washes each sheet back and forth by 90 of per minutes and carries net 5-8 time, blots residue distilled water with filter paper, dries promptly to can be used for electron microscopic observation.
The present invention compared with prior art has the following advantages:
(1) craft that reduces in the dyeing course repeats loaded down with trivial details operation, removes to cause pollution too greatly because of the pure manual operations mistiming;
(2) can carry out secondary (or repeatedly) dyeing in same position, that is: acetic acid uranium dyeing, plumbous dyeing;
(3) for the generation of acetic acid uranium dyeing can the minimizing effectively pollutant, can reduce the generation of about 90% pollutant;
(4) can carry out the electron microscope sample negative staining;
(5) can carry out electron microscopic sample hatches after histochemical;
(6) keep the pollution-free of sample to greatest extent, obtain the result of best quality;
(7) practice thrift the consumption etc. of acetic acid uranium, double distilled water effectively.
The apparatus that is used for the section statining of electron microscope biological sample of the present invention; Utilize the location turnover panel to add location snap close function technology, repeatedly dye before observing electron microscope sample, (histochemistry hatches but especially coordination repeatedly dyes; The dyeing of acetic acid uranium; Plumbous dyeing) etc., need not to carry out repeatedly manual operations, can reduce the mistiming and the generation of destroy contaminants; Give security for obtaining high-quality experimental result, the preparation macromolecule sample of hatching, reach that apparatus of the present invention can be used for the SABC of histochemistry's electron microscope drips sheet etc.
Parts that apparatus designed and the preparation material thereof that is used for the section statining of electron microscope biological sample of the present invention all can be purchased through market; Further combine structure shown in Figure 1, conventional preparation method prepares the described apparatus that is used for the section statining of electron microscope biological sample through this area.
The present invention prepares the described apparatus that is used for the section statining of electron microscope biological sample through following method:
As shown in Figure 1, control built-in turnover panel and have a secluding air flow-through grooves but described apparatus is provided with snap close, its by position fixing knob 1, water delivering orifice 2, groove turnover panel 3, lath 4, built-in uncovered box 5, top cover 6, stablize packing ring 7 and truss 8 is formed; Described groove turnover panel 3 places in the top groove of truss 8; Position fixing knob 1 is located at truss 8 outsides, its position is corresponding with groove turnover panel 3; Described lath 4 places the top of groove turnover panel 3; Described built-in uncovered box 5 is positioned at the top groove of truss 8, a side of groove turnover panel 3, and top cover 6 covers on the top groove of truss 8, and the bottom of truss 8 is provided with for four jiaos stablizes packing ring 7.The locating button of 30 ℃, 45 ℃ and 60 ℃ location of ability is set on the described position fixing knob 1; Establish valve on the water delivering orifice 2; Groove turnover panel 3 places in the groove of truss 8, and this groove turnover panel 3 is rotatable; Lath 4 employing silica gel are processed, and it is provided with is with the location to carry net embedding mouth, and this lath 4 is protruding embedded for down, on this lath, sets several and is colored a section year net; Described built-in uncovered box 5 is made for movable, is used to insert sodium hydrate particle; Described top cover 6 is processed button-type, and its surface is provided with handle 9; Adopt rubber to process and stablize packing ring 7; Adopt stainless steel to process truss 8, groove is established on its top, and left 16cm place is a boundary 10 in the groove.
For the ease of understanding, through accompanying drawing and specific embodiment the apparatus that is used for the section statining of electron microscope biological sample of the present invention is carried out detailed description below.What need particularly point out is, specific embodiment and accompanying drawing only are in order to explain that obviously those skilled in the art can explain according to this paper, and the present invention is carried out various corrections or change, and these corrections and changing also will be included within the scope.
Description of drawings
Fig. 1 is a structural representation of the present invention,
Wherein, the 1st, position fixing knob, the 2nd, water delivering orifice, the 3rd, the groove turnover panel, the 4th, lath, the 5th, built-in uncovered box, the 6th, top cover, the 7th, stablize packing ring, the 8th, truss, the 9th, handle, the 10th, boundary.
Embodiment
Embodiment 1
As shown in Figure 1; Apparatus among the present invention is to utilize the location turnover panel to add location snap close function technology; To repeatedly dye before the electron microscope sample observation (dyeing of acetic acid uranium, plumbous dyeing, negative staining); But described apparatus is provided with snap close and controls dyeing apparatus built-in turnover panel, that have the secluding air flow-through grooves, its by position fixing knob 1, water delivering orifice 2, groove turnover panel 3, lath 4, built-in uncovered box 5, top cover 6, stablize packing ring 7 and truss 8 is formed; Described groove turnover panel 3 places in the top groove of truss 8; Position fixing knob 1 is located at truss 8 outsides, its position is corresponding with groove turnover panel 3; Described lath 4 places the top of groove turnover panel 3; Described built-in uncovered box 5 is positioned at the top groove of truss 8, a side of groove turnover panel 3, and top cover 6 covers on the top groove of truss 8, and the bottom of truss 8 is provided with for four jiaos stablizes packing ring 7.Described position fixing knob 1 length is 4cm, is provided with the locating button of 30 ℃, 45 ℃ and 60 ℃ location of ability; Described water delivering orifice 2 is provided with valve, is used for getting rid of the solution of groove, and its diameter valve is 0.5cm; Described groove turnover panel 3 is built in the groove of truss 8, and this groove turnover panel 3 is rotatable, its length be 16cm, width be 0.6cm, highly for 0.3cm; Described lath 4 adopts silica gel to process; It is provided with is with the location to carry net embedding mouth; This lath 4 is protruding embedded for down; Its lower bottom part length is 16cm, the wide 0.6cm of being, the high 0.3cm of being, upper base length is that cm, height are the silica gel strip of 0.3cm (type paraffin plate function) for 16cm, wide 1.2, on this lath, can set 16 and be colored year net of cutting into slices; Described built-in uncovered box 5 is movable, and its length is that 3.5cm, width are 1.6cm, highly are to be used to insert sodium hydrate particle by 1.0cm; Described top cover 6 is a button-type, and physical dimension is 22.4cm*2.2cm*0.8cm, and wherein, the following length of side of its button-type interface is 22.4cm, the wide 2.2cm of being, the high 0.3cm of being, the last length of side of button-type interface is 22cm, the wide 1.8cm of being, the high 0.5cm of being; The surface on described top 6 is provided with the handle 9 that physical dimension is 5cm*1.0cm*0.5cm; Described stable packing ring 7 has four, adopts rubber to process; Described truss 8 adopts stainless steels to process, and its physical dimension is: long for 22cm, highly be 8cm, wherein, the seat end wide be 6cm, thickly be 0.5cm, the top of truss 8 is long be 22cm, wide be 1.8cm; Described truss 8 tops are provided with groove; The length of this inside grooves is 20cm; Its left 16cm place is a boundary 10, and groove reprint 3 is established in boundary 10 left sides, built-in uncovered box 5 is established on the right side, and the height of boundary 10 is 1.8cm; The height in boundary 10 left sides is 2.3cm, width 1.8cm, and the height on boundary 10 right sides is that 1.8cm, width are 1.8cm.
Instrumentation of the present invention is easy; What will have the biological sample section during use in order carries net (copper mesh, nickel screen etc.) with the embedding mouth location on the angle embedding lath 4 of 45 or 90 degree; And with on the lath 4 placement groove turnover panels 3; Add the carbon dioxide that sodium hydrate particle is used to absorb local space for 5 li at built-in uncovered capsule, produce sediment to reduce the reaction of carbon dioxide and plumbous dyeing liquor; Top cover 6 was covered 1-2 minute; Taking down top cover 6 needs to carry net biologic slice one side having of dyeing at every, drips fast with pipettor and goes up dyeing liquor bonnet upper top cover 5-8 minute; Take down top cover 6, positioning of rotating handle 1 makes the location snap into miter angle, gets distilled water with garter drop method, washes each sheet back and forth by 90 of per minutes and carries net 5-8 time, blots residue distilled water with filter paper, dries to get final product electron microscopic observation.
But the present invention's coordination repeatedly dyes (histochemistry hatches, acetic acid uranium dyeing, plumbous dyeing) etc., need not to carry out repeatedly manual operations, can reduce the mistiming, the generation of destroy contaminants, give security for obtaining high-quality experimental result.
Claims (10)
1. apparatus that is used for the section statining of electron microscope biological sample; It is characterized in that; Comprise that but snap close controls built-in turnover panel and secluding air flow-through grooves, by position fixing knob (1), water delivering orifice (2), groove turnover panel (3), lath (4), built-in uncovered box (5), top cover (6), stablize packing ring (7) and truss (8) is formed; Described groove turnover panel (3) places in the top groove of truss (8); Position fixing knob (1) is located at truss (8) outside, its position is corresponding with groove turnover panel (3); Described lath (4) places the top of groove turnover panel (3); Described built-in uncovered box (5) is positioned at the top groove of truss (8), a side of groove turnover panel (3), and top cover (6) covers on the top groove of truss (8), and the bottom of truss (8) is provided with stable packing ring (7) for four jiaos.
2. by the described apparatus that is used for the section statining of electron microscope biological sample of claim 1, it is characterized in that described position fixing knob (1) length is 4cm, position fixing knob is provided with the locating button of 30 ℃, 45 ℃ and 60 ℃ location.
3. by the described apparatus that is used for the section statining of electron microscope biological sample of claim 1, it is characterized in that described water delivering orifice (2) is provided with valve, this diameter valve is 0.5cm.
4. by the described apparatus that is used for the section statining of electron microscope biological sample of claim 1, it is characterized in that described groove turnover panel (3) is rotation groove turnover panel, its length is that 16cm, width are 0.6cm, highly are 0.3cm.
5. by the described apparatus that is used for the section statining of electron microscope biological sample of claim 1, it is characterized in that described lath (4) is protruding embedded down; This lath (4) is provided with is with the location to carry net embedding mouth.
6. by the described apparatus that is used for the section statining of electron microscope biological sample of claim 1, it is characterized in that, its length of described built-in uncovered box (5) be 3.5cm, width be 1.6cm, highly for 1.0cm.
7. by the described apparatus that is used for the section statining of electron microscope biological sample of claim 1, it is characterized in that described top cover (6) is a button-type, its surface is provided with handle (9).
8. by the described apparatus that is used for the section statining of electron microscope biological sample of claim 7, it is characterized in that the physical dimension of described top cover (6) is 22.4cm*2.2cm*0.8cm.
9. by the described apparatus that is used for the section statining of electron microscope biological sample of claim 1, it is characterized in that the top of described truss (8) is provided with groove, this inside grooves left 16cm place is provided with boundary (10).
10. by the described apparatus that is used for the section statining of electron microscope biological sample of claim 9, it is characterized in that groove reprint (3) is established in its left side of described boundary (10), built-in uncovered box (5) is established on the right side.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110063801.9A CN102680303B (en) | 2011-03-11 | 2011-03-11 | Instrument for sectioning and dyeing biological sample on electronic microscope |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110063801.9A CN102680303B (en) | 2011-03-11 | 2011-03-11 | Instrument for sectioning and dyeing biological sample on electronic microscope |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102680303A true CN102680303A (en) | 2012-09-19 |
| CN102680303B CN102680303B (en) | 2015-06-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201110063801.9A Expired - Fee Related CN102680303B (en) | 2011-03-11 | 2011-03-11 | Instrument for sectioning and dyeing biological sample on electronic microscope |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6262243A (en) * | 1985-09-12 | 1987-03-18 | Omron Tateisi Electronics Co | Automatic blood specimen preparing apparatus |
| US20060178776A1 (en) * | 2003-12-15 | 2006-08-10 | Feingold Gordon A | Systems and methods for the automated pre-treatment and processing of biological samples |
| CN101029858A (en) * | 2007-03-16 | 2007-09-05 | 首都医科大学北京神经科学研究所 | Apparatus for dyeing electron microscope specimen |
| CN202066731U (en) * | 2011-03-11 | 2011-12-07 | 复旦大学 | Instrument used for slicing and staining biological samples of electron microscope |
-
2011
- 2011-03-11 CN CN201110063801.9A patent/CN102680303B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6262243A (en) * | 1985-09-12 | 1987-03-18 | Omron Tateisi Electronics Co | Automatic blood specimen preparing apparatus |
| US20060178776A1 (en) * | 2003-12-15 | 2006-08-10 | Feingold Gordon A | Systems and methods for the automated pre-treatment and processing of biological samples |
| CN101029858A (en) * | 2007-03-16 | 2007-09-05 | 首都医科大学北京神经科学研究所 | Apparatus for dyeing electron microscope specimen |
| CN202066731U (en) * | 2011-03-11 | 2011-12-07 | 复旦大学 | Instrument used for slicing and staining biological samples of electron microscope |
Non-Patent Citations (2)
| Title |
|---|
| 吴友吕: "《用于电子显微镜微生物标本锇酸蒸汽固定和超薄切片的结晶碘蒸汽染色的一种装置》", 《微生物学报》 * |
| 吴洪娟: "《防污染的良超薄切片电子染色法》", 《潍坊医学院学报》 * |
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| CN102680303B (en) | 2015-06-17 |
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