CN102565131A - Biosensing tester and biosensing test system - Google Patents
Biosensing tester and biosensing test system Download PDFInfo
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- CN102565131A CN102565131A CN2010106052923A CN201010605292A CN102565131A CN 102565131 A CN102565131 A CN 102565131A CN 2010106052923 A CN2010106052923 A CN 2010106052923A CN 201010605292 A CN201010605292 A CN 201010605292A CN 102565131 A CN102565131 A CN 102565131A
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Abstract
The invention discloses a biosensing tester and a biosensing test system. The biosensing tester comprises a first operational amplifier, a second operational amplifier, a first reference voltage source, a second reference voltage source, a detection device, a first port, a second port, a third port and a fourth port; the first input of the first operational amplifier is connected with the first reference voltage source, and the second input of the first operational amplifier is connected with the second port; the output of the first operational amplifier is connected with the first port and the detection device; the first input of the second operational amplifier is connected with the second reference voltage source, and the second input of the second operational amplifier is connected with the fourth port; and the output of the second operational amplifier is connected with the third port. According to the biosensing tester and the biosensing test system disclosed by the invention, the biosensing testing precision can be effectively improved.
Description
Technical field
The present invention relates to the bio-sensing field tests, specifically, relate to a kind of bio-sensing tester and bio-sensing test macro.
Background technology
At present, the bio-sensor system that is used for the test signal relevant with biological fluid content of analyte (such as blood-sugar content) comprises the bio-sensing test-strips and uses the test macro of this test-strips.Fig. 1 is a typical bio-sensing test-strips; This bio-sensing test-strips is by the biological reagent 18 on substrate 12, substrate one end, form with the conductive region 14,16 of biological reagent 18 conductings.It is poor between test electrode 14a, 16a, to apply a constant potential, so that the electric signal that the reaction of the measured matter in detection of biological reagent 18 and the biological sample is produced.
The shortcoming of the bio-sensing test-strips of this structure is: even constant through the current potential that applies between the sub-14b of electrode tips, the 16b; Because all there is dead resistance in any conductive material, through conductive channel 14c, 16c and the actual potential difference (PD) that puts between test electrode 14a, the 16a is not the potential difference (PD) that is applied; And because that the dead resistance of bio-sensing test-strips can not be accomplished is in full accord, so the actual potential difference (PD) that puts between test electrode 14a, the 16a can not be accomplished constant.
Summary of the invention
The technical problem underlying that the present invention will solve is, a kind of bio-sensing tester and bio-sensing test macro are provided, and can effectively improve the bio-sensing measuring accuracy.
For solving the problems of the technologies described above, the present invention has adopted following technical scheme:
A kind of bio-sensing tester comprises:
First operational amplifier, second operational amplifier, first reference voltage source, second reference voltage source, detection means, and first port, second port, the 3rd port, the 4th port; First input of said first operational amplifier connects said first reference voltage source, and second input connects said second port, and the output of said first operational amplifier connects said first port, and connects said detection means; First input of said second operational amplifier connects said second reference voltage source, and second input connects said the 4th port, and the output of said second operational amplifier connects said the 3rd port.
In a kind of embodiment of said bio-sensing tester, said bio-sensing tester also comprises resistance, and said resistance is connected between the output and said first port of said first operational amplifier.
In a kind of embodiment of said bio-sensing tester, when the resistance of said resistance is biological sensing testing and more than 10 times of dead resistance of the bio-sensing test-strips that is used of said bio-sensing tester.
In a kind of embodiment of said bio-sensing tester, said first reference voltage source and second reference voltage source are digital to analog converter.
In a kind of embodiment of said bio-sensing tester, said detection means is an analog to digital converter.
In a kind of embodiment of said bio-sensing tester; Also comprise microprocessor; Have first control output end that links to each other with said first reference voltage source and second control output end that links to each other with said second reference voltage source, set in order to the output potential of controlling said first reference voltage source and said second reference voltage source; Said microprocessor also has the input end that links to each other with said detection means; Said first reference voltage source and second reference voltage source are the digital to analog converter of said microprocessor internal, and said detection means is the analog to digital converter of said microprocessor internal.
The present invention also provides a kind of bio-sensing test macro; Comprise the bio-sensing test-strips and cooperate with said bio-sensing test-strips and the bio-sensing tester that carries out the bio-sensing test that said bio-sensing test-strips comprises first test electrode, first conductive channel that is communicated with first test electrode, second conductive channel, second test electrode, the 3rd conductive channel that is communicated with second test electrode, the 4th conductive channel; Said bio-sensing tester comprises first operational amplifier, second operational amplifier, first reference voltage source, second reference voltage source, detection means, and first port, second port, the 3rd port, the 4th port; First input of said first operational amplifier connects said first reference voltage source, and second input connects said second port, and the output of said first operational amplifier connects said first port, and connects said detection means; First input of said second operational amplifier connects said second reference voltage source, and second input connects said the 4th port, and the output of said second operational amplifier connects said the 3rd port; During use, first port and the coupling of said first conductive channel, second port and the coupling of said second conductive channel, the 3rd port and the coupling of said the 3rd conductive channel, the 4th port and the coupling of said the 4th conductive channel.
In a kind of embodiment of said bio-sensing test macro, said bio-sensing tester also comprises resistance, and said resistance is connected between the output and said first port of said first operational amplifier.
In a kind of embodiment of said bio-sensing test macro, the resistance of said resistance is more than 10 times of dead resistance of said bio-sensing test-strips.
In a kind of embodiment of said bio-sensing test macro, said first reference voltage source and second reference voltage source are digital to analog converter; Said detection means is an analog to digital converter.
When carrying out the bio-sensing test, biological reagent and biological analyte reaction generate little electric current, but because operational amplifier has very large input impedance, and little electric current can only flow to or flow out the output of first operational amplifier, promptly on first conductive channel, flow; There is not electric current to flow and connect on second conductive channel of first operational amplifier second input; That is to say; Current potential on second conductive channel is consistent; First test electrode is identical with the current potential of second input of first operational amplifier, and the current potential of first test electrode can be set through digital to analog converter D/A334 accurately, guarantees that the actual voltage difference that is applied on the bio-sensing test-strips is required voltage difference; Make the current potential that the test electrode of bio-sensing test-strips obtains setting, guaranteed the precision of bio-sensing test.
Further, through resistance in series between first conductive channel of the output of first operational amplifier and bio-sensing test-strips, because resistance in series is far longer than dead resistance usually; For example, in described a kind of embodiment, resistance in series is set to be at least more than 10 times of dead resistance; Thereby the pressure drop that forms on the dead resistance will significantly reduce; Pressure drop will mainly focus on resistance in series, and during the electric signal of the output detection of biological sensing through first operational amplifier, the influence to electrical signal detection of dead resistance will reduce greatly like this; Thereby reduced the influence of dead resistance to measuring accuracy, further improved the bio-sensing measuring accuracy.
Description of drawings
Fig. 1 is a kind of typical bio-sensing test-strips;
Fig. 2 is the employed bio-sensing test-strips of the embodiment of the invention;
Fig. 3 is the bio-sensing tester of an embodiment of the present invention;
Fig. 4 is the bio-sensing tester of the another kind of embodiment of the present invention.
Embodiment
Combine accompanying drawing that the present invention is done further explain through embodiment below.
The present invention relates to a kind of bio-sensing tester and bio-sensing test macro.Said bio-sensing tester and bio-sensing test macro are used for test and biological fluid content of analyte---such as the relevant signal of blood-sugar content; Also can be used for measuring and disturbing---such as the signal of hematocrit in the blood sugar and temperature correlation, so that analyze the content of analyte signal.This bio-sensing tester and bio-sensing test macro that the embodiment of the invention proposed; (it is poor to be generally constant potential with required potential difference (PD) for ability; But it is poor to be not limited to constant potential) be applied to the test electrode of reaction zone; And compensate the dead resistance of bio-sensing test-strips conductive channel simultaneously, thereby reduce of the influence of the dead resistance of bio-sensing test-strips as much as possible measuring accuracy.
Referring to Fig. 2, employed bio-sensing test-strips comprises in the embodiment of the invention: substrate 12 generally can be the thick film of 350uM, like PET or PVC etc.; Working electrode 226,214 reaches electrode 216,224, can be made through technologies such as printing or plating; Wherein, label 226 is as the general designation of label 226a, 226b, 226c; Label 214 is as the general designation of label 214a, 214b, 214c; Label 216 is as the general designation of label 216a, 216b, 216c; Label 224 is as the general designation of label 224a, 224b, 224c.The implication of label a, b, c is following:
Test electrode a---the electrode part in the reaction zone: 226a, 214a, 216a, 224a;
Contact terminal b---the part that is connected with the bio-sensing tester: 226b, 214b, 216b, 224b;
Bus c---connect the part of test electrode and contact terminal: 226c, 214c, 216c, 224c;
The bca of above-mentioned each electrode or acb constitute each conductive channel of bio-sensing test-strips; Wherein, 226b, 226c, 226a constitute first conductive channel; 214b, 214c, 214a constitute second conductive channel, and 216b, 216c, 216a constitute the 3rd conductive channel, and 224b, 224c, 224a constitute the 4th conductive channel.The first test electrode 226a, 214a are communicated with first conductive channel, second conductive channel; The second test electrode 216a, 224a are communicated with the 3rd conductive channel, the 4th conductive channel.
Biological reagent 18 is attached to test electrode 216a, the 214a of bio-sensing test-strips one end through printing or spraying, technologies such as some liquid etc.When give test electrode 216a, when 214a adds a potential difference (PD), the biological analyte reaction of biological reagent 18 and required mensuration content can be measured the content of said biological analyte reaction.
The bio-sensing test-strips has a reaction zone 20.Reaction zone 20 comprises test electrode 214a, 216a, and test electrode directly contacts with the sample of the biological analyte of required mensuration content.In whole electro-chemical test system; The bio-sensing test-strips is inserted in the tester that uses this test-strips; Test electrode 214a in the reaction zone 20,216a are connected with Fig. 3 or bio-sensing tester shown in Figure 4; The bio-sensing tester provides a potential difference (PD) (it is poor to be generally constant potential, but it is poor to be not limited to constant potential) for test electrode 214a, 216a, and measures the reaction of electrochemical sensor to this potential difference (PD).This reaction and content of analyte are proportional.
The bio-sensing test-strips has contact region 22.Contact region 22 comprises contact terminal 226b, 214b, 216b, 224b.Contact region 22 usually but may not be certain, be positioned at away from the end on the bio-sensing test-strips of reaction zone 20.
The bio-sensing tester connects the bio-sensing test-strips through contact terminal 226b, 214b, 216b, 224b.Bus 226c, 214c, 216c, 224c connect contact terminal 226b, 214b, 216b, 224b and test electrode 226a, 214a, 216a, 224a respectively.
Fig. 3 is the circuit diagram of the bio-sensing tester of embodiment one; Test electrode 214a, the 216a that the test circuit of bio-sensing tester is comprised for the reaction zone 20 of bio-sensing test-strips applies required potential difference (PD), and (it is poor to be generally constant potential; But it is poor to be not limited to constant potential); And compensate the dead resistance of the conductive channel of bio-sensing test-strips simultaneously, thereby reduce the influence of dead resistance as much as possible measuring accuracy.
When the bio-sensing test-strips is inserted the bio-sensing tester; First port of bio-sensing tester is connected to the contact terminal 226b of bio-sensing test-strips; Second port is connected to the contact terminal 214b of bio-sensing test-strips; The 3rd port is connected to the contact terminal 216b of bio-sensing test-strips, and the 4th port is connected to the contact terminal 224b of bio-sensing test-strips.
Second operational amplifier 320 of the test circuit of bio-sensing tester is formed voltage follower with electrode 216,224.First input of second operational amplifier 320 connects second reference voltage source (in the example of Fig. 3; Be digital to analog converter D/A336); Second input connects the 4th port that is used to connect bio-sensing test-strips contact terminal 224b, and output connects the 3rd port that is used to connect bio-sensing test-strips contact terminal 216b.Second reference voltage source, i.e. D/A336 output required voltage is supplied with said voltage follower, thus the test electrode 216a that follows path that is positioned at voltage follower keeps exporting same voltage with D/A522.
First input of first operational amplifier 310 of the test circuit of bio-sensing tester connects first reference voltage source (in the example of Fig. 3; Be digital to analog converter D/A334); Second input of first operational amplifier 310 connects second port that is used to connect bio-sensing test-strips contact terminal 214b, and the output of first operational amplifier 310 connects first port that is used to connect bio-sensing test-strips contact terminal 226b.In the inside of tester circuit, the output of first operational amplifier 310 is also connected to a detection means (in the example of Fig. 3, promptly modulus converter A/D 332).D/A334 output required voltage is supplied with first input of first operational amplifier 310, and the current potential of second input of first operational amplifier 310 equates with the current potential of its first input.The biological reagent of reaction zone 20 and biological analyte reaction; Generate little electric current; But because operational amplifier has very large input impedance, little electric current can only flow to or flow out the output of first operational amplifier 310, promptly on first conductive channel that 226b, 226c, 226a constitute, flows; And do not have electric current to flow on second conductive channel that the 214a, 214c, the 214b that connect 310 second inputs of first operational amplifier constitute; That is to say; Current potential on 214a, 214c, the 214b conductive channel is consistent, and promptly test electrode 214a is identical with the current potential of second input of first operational amplifier 310.Test electrode 226a is identical with the current potential of first input of first operational amplifier 310, is the current potential that digital to analog converter D/A334 is exported, and the current potential of test electrode 214a is the current potential of electrode 226a, can set through digital to analog converter D/A334.
Therefore can pass through digital to analog converter D/A334,336 between test electrode 214a, the 216a and set required potential difference (PD).The setting of potential difference (PD), test result are come out mainly by microprocessor processes; Microprocessor has first control output end that links to each other with first reference voltage source (digital to analog converter D/A334) and second control output end that links to each other with second reference voltage source (digital to analog converter D/A336), sets in order to the output potential of controlling first reference voltage source and second reference voltage source; Microprocessor also has the input end that links to each other with detection means (modulus converter A/D 332); Can digital to analog converter D/A334,336, digital to analog converter D/A336 etc. all be arranged on the inside of microprocessor; Promptly first reference voltage source and second reference voltage source are the digital to analog converter of microprocessor internal, and detection means is the analog to digital converter of microprocessor internal.
There are dead resistance in conductive channel 226b, 226c, 226a, and because technological reason, this dead resistance of bio-sensing test-strips can not be accomplished unanimity.Referring to Fig. 4; Can be between the output of first operational amplifier of bio-sensing tester and first port resistance in series 440; And make the dead resistance of the resistance of this resistance in series 440 much larger than (for example more than 10 times) conductive channel 226b, 226c, 226a; Be the pressure drop that on resistance 440, produces of little electric current much larger than the pressure drop that on dead resistance, produces, thereby can control dead resistance institute errors caused.
Above content is to combine concrete embodiment to the further explain that the present invention did, and can not assert that practical implementation of the present invention is confined to these explanations.For the those of ordinary skill of technical field under the present invention, under the prerequisite that does not break away from the present invention's design, can also make some simple deduction or replace, all should be regarded as belonging to protection scope of the present invention.
Claims (10)
1. a bio-sensing tester is characterized in that, comprising:
First operational amplifier, second operational amplifier, first reference voltage source, second reference voltage source, detection means, and first port, second port, the 3rd port, the 4th port; First input of said first operational amplifier connects said first reference voltage source, and second input connects said second port, and the output of said first operational amplifier connects said first port, and connects said detection means; First input of said second operational amplifier connects said second reference voltage source, and second input connects said the 4th port, and the output of said second operational amplifier connects said the 3rd port.
2. bio-sensing tester as claimed in claim 1 is characterized in that, said bio-sensing tester also comprises resistance, and said resistance is connected between the output and said first port of said first operational amplifier.
3. bio-sensing tester as claimed in claim 2 is characterized in that, when the resistance of said resistance is biological sensing testing and more than 10 times of dead resistance of the bio-sensing test-strips that is used of said bio-sensing tester.
4. bio-sensing tester as claimed in claim 1 is characterized in that, said first reference voltage source and second reference voltage source are digital to analog converter.
5. bio-sensing tester as claimed in claim 1 is characterized in that, said detection means is an analog to digital converter.
6. like the arbitrary described bio-sensing tester of claim 1-5; It is characterized in that; Also comprise microprocessor; Have first control output end that links to each other with said first reference voltage source and second control output end that links to each other with said second reference voltage source, set in order to the output potential of controlling said first reference voltage source and said second reference voltage source; Said microprocessor also has the input end that links to each other with said detection means, and said first reference voltage source and second reference voltage source are the digital to analog converter of said microprocessor internal, and said detection means is the analog to digital converter of said microprocessor internal.
7. bio-sensing test macro; It is characterized in that; Comprise the bio-sensing test-strips and cooperate with said bio-sensing test-strips and the bio-sensing tester that carries out the bio-sensing test that said bio-sensing test-strips comprises first test electrode, first conductive channel that is communicated with first test electrode, second conductive channel, second test electrode, the 3rd conductive channel that is communicated with second test electrode, the 4th conductive channel; Said bio-sensing tester comprises first operational amplifier, second operational amplifier, first reference voltage source, second reference voltage source, detection means, and first port, second port, the 3rd port, the 4th port; First input of said first operational amplifier connects said first reference voltage source, and second input connects said second port, and the output of said first operational amplifier connects said first port, and connects said detection means; First input of said second operational amplifier connects said second reference voltage source, and second input connects said the 4th port, and the output of said second operational amplifier connects said the 3rd port; During use, first port and the coupling of said first conductive channel, second port and the coupling of said second conductive channel, the 3rd port and the coupling of said the 3rd conductive channel, the 4th port and the coupling of said the 4th conductive channel.
8. bio-sensing test macro as claimed in claim 7 is characterized in that, said bio-sensing tester also comprises resistance, and said resistance is connected between the output and said first port of said first operational amplifier.
9. bio-sensing test macro as claimed in claim 8 is characterized in that, the resistance of said resistance is more than 10 times of dead resistance of said bio-sensing test-strips.
10. like the arbitrary described bio-sensing test macro of claim 7-9, it is characterized in that said first reference voltage source and second reference voltage source are digital to analog converter; Said detection means is an analog to digital converter.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010605292 CN102565131B (en) | 2010-12-24 | 2010-12-24 | Biosensing test system |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201010605292 CN102565131B (en) | 2010-12-24 | 2010-12-24 | Biosensing test system |
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| CN102565131A true CN102565131A (en) | 2012-07-11 |
| CN102565131B CN102565131B (en) | 2013-09-04 |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2528010Y (en) * | 2002-04-02 | 2002-12-25 | 泰博科技股份有限公司 | Biological sensor |
| US20030203498A1 (en) * | 2002-04-25 | 2003-10-30 | Home Diagnostics, Inc. | System and methods for blood glucose sensing |
| US20050279631A1 (en) * | 2004-06-18 | 2005-12-22 | Celentano Michael J | System and method for quality assurance of a biosensor test strip |
| US20080093227A1 (en) * | 2006-10-18 | 2008-04-24 | Agamatrix, Inc. | Error detection in analyte measurements based on measurement of system resistance |
| US20080112852A1 (en) * | 2002-04-25 | 2008-05-15 | Neel Gary T | Test Strips and System for Measuring Analyte Levels in a Fluid Sample |
| CN201903525U (en) * | 2010-12-24 | 2011-07-20 | 深圳市金亿帝科技有限公司 | Biological sensing test instrument and biological sensing test system |
-
2010
- 2010-12-24 CN CN 201010605292 patent/CN102565131B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2528010Y (en) * | 2002-04-02 | 2002-12-25 | 泰博科技股份有限公司 | Biological sensor |
| US20030203498A1 (en) * | 2002-04-25 | 2003-10-30 | Home Diagnostics, Inc. | System and methods for blood glucose sensing |
| US20080112852A1 (en) * | 2002-04-25 | 2008-05-15 | Neel Gary T | Test Strips and System for Measuring Analyte Levels in a Fluid Sample |
| US20050279631A1 (en) * | 2004-06-18 | 2005-12-22 | Celentano Michael J | System and method for quality assurance of a biosensor test strip |
| US20080093227A1 (en) * | 2006-10-18 | 2008-04-24 | Agamatrix, Inc. | Error detection in analyte measurements based on measurement of system resistance |
| CN201903525U (en) * | 2010-12-24 | 2011-07-20 | 深圳市金亿帝科技有限公司 | Biological sensing test instrument and biological sensing test system |
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| CN102565131B (en) | 2013-09-04 |
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