CN102393358B - The measuring method of chemical liquid luminescence intensity - Google Patents
The measuring method of chemical liquid luminescence intensity Download PDFInfo
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- CN102393358B CN102393358B CN201110411503.4A CN201110411503A CN102393358B CN 102393358 B CN102393358 B CN 102393358B CN 201110411503 A CN201110411503 A CN 201110411503A CN 102393358 B CN102393358 B CN 102393358B
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Abstract
The present invention relates to the measuring method of a kind of chemiluminescence measuring instrument and chemical liquid luminescence intensity.Existing chemiluminescence measuring instrument volume is large, measuring speed is slow.For this reason, this chemiluminescence measuring instrument comprises microwell plate, photoelectric sensor and microwell plate drive system, microwell plate drive system can drive microwell plate to vertically move, it is characterized in that: it also comprises light transmitting fiber, this light transmitting fiber one end is fixed on the input end of described photoelectric sensor, its other end is mobile terminal, is furnished with transversal driving mechanism, and this transversal driving mechanism can drive mobile terminal to move laterally above microwell plate.As optimization, when light transmitting fiber mobile terminal is moved relative to microwell plate, back and forth movement or by column back and forth movement line by line.It is little that chemiluminescence measuring instrument of the present invention has volume, the advantage that measuring speed is fast, efficiency is high, and the measuring method of chemical liquid luminescence intensity of the present invention is all measured at round trip, and speed is fast, efficiency is high, is applicable to biological chemistry and clinical diagnosis field.
Description
Technical field
The present invention relates to the measuring method of a kind of chemiluminescence measuring instrument and chemical liquid luminescence intensity.
Background technology
Chemiluminescence measurement utilizes instrument catching and analyzing faint optical signal, is widely used at present in biological chemistry and clinical diagnosis field.Instrument is optics, machinery, and electronics controls the product of multi-field combine with technique automatically.
Under the low light level, photomultiplier output signal has into the feature of nature discretize, and when low light irradiation is to photomultiplier transit tube cathode, each incident photon makes photocathode launch an electronics with certain probability (i.e. quantum efficiency).This photoelectron is through the multiplication of dynode system, and finally in plate tank, form a current impulse, form a potential pulse by pull-up resistor, this pulse is called single photon pulses.According to the nonoverlapping photonic energy pulse calculating gained, the luminous intensity of fluid to be measured can be known.
Existing chemiluminescence measuring instrument is all by mobile microwell plate, and hole-specifically pause and measure testing liquid, measuring speed is slow, and its photomultiplier is perpendicular to pedestal, and volume is large.
Summary of the invention
The technical problem to be solved in the present invention how to overcome the defect that existing chemiluminescence measuring instrument volume is large, measuring speed is slow, provides the measuring method of a kind of chemiluminescence measuring instrument and chemical liquid luminescence intensity.
For solving the problems of the technologies described above, this chemiluminescence measuring instrument comprises microwell plate, photoelectric sensor and microwell plate drive system, microwell plate drive system can drive microwell plate to vertically move, it is characterized in that: it also comprises light transmitting fiber, this light transmitting fiber one end is fixed on the input end of described photoelectric sensor, its other end is mobile terminal, is furnished with transversal driving mechanism, and this transversal driving mechanism can drive mobile terminal to move laterally above microwell plate.Design like this, microwell plate drive system is only equipped with longitudinal moving mechanism, and transverse shifting is driven by the transversal driving mechanism that volume is little, and microwell plate transverse shifting stroke reduces, and instrument entirety is long-pending to be reduced, and measuring speed is accelerated greatly.
As optimization, described microwell plate evenly has 8 × 12=96 micropore, longitudinal often row 12 micropores, laterally often arrange totally 8 micropores.
As optimization, described photoelectric sensor is end window type photomultiplier.
As optimization, described photomultiplier is laterally fixing.Design like this, described photomultiplier is parallel to pedestal, and volume is less.
The measuring method of chemical liquid luminescence intensity of the present invention, comprises the steps:
1) testing liquid is injected in the micropore of the luminous measuring instrument microwell plate of afore mentioned chemical;
2) microwell plate drive system and transversal driving mechanism in the luminous measuring instrument of afore mentioned chemical is alternately started, "the" shape motion is done relative to microwell plate in light transmitting fiber mobile terminal, time not stopping continuously and slip over each micropore in light transmitting fiber mobile terminal, photoelectric sensor gathers photon count value.When testing liquid measured by existing chemiluminescence measuring instrument, photoelectric sensor and microwell plate generation relative movement, need pause to every hole and measure.Design like this, when photoelectric sensor of the present invention and microwell plate generation relative movement, do not stop continuous coverage, and efficiency is higher.
As optimization, the 2nd) when step light transmitting fiber mobile terminal is moved relative to microwell plate, back and forth movement line by line.
As optimization, the 2nd) when step light transmitting fiber mobile terminal is moved relative to microwell plate, back and forth movement by column.
It is little that chemiluminescence measuring instrument of the present invention has volume, the advantage that measuring speed is fast, efficiency is high, and the measuring method of chemical liquid luminescence intensity of the present invention is all measured at round trip, and speed is fast, efficiency is high, is applicable to biological chemistry and clinical diagnosis field.
Accompanying drawing explanation
Be described further below in conjunction with the measuring method of accompanying drawing to chemiluminescence measuring instrument of the present invention and chemical liquid luminescence intensity:
Fig. 1 is chemiluminescence measuring instrument perspective view of the present invention;
Fig. 2 is that schematic diagram is moved towards relative to the one of microwell plate in the measuring method light transmitting fiber mobile terminal of this chemical liquid luminescence intensity;
Fig. 3 is that schematic diagram is moved towards relative to the another kind of microwell plate in the measuring method light transmitting fiber mobile terminal of this chemical liquid luminescence intensity.
In figure: 1 be microwell plate, 2 be photoelectric sensor, 3 be light transmitting fiber, 31 be mobile terminal, 4 be transverse shifting servomotor, 5 for micropore.
Embodiment
Embodiment one: as Figure 1-3, this chemiluminescence measuring instrument comprises microwell plate 1, photoelectric sensor 2 and microwell plate drive system, microwell plate drive system can drive microwell plate 1 to vertically move (X-direction namely in Fig. 1), it is characterized in that: it also comprises light transmitting fiber 3, this light transmitting fiber 3 one end is fixed on the input end of described photoelectric sensor 2, its other end is mobile terminal 31, is furnished with transversal driving mechanism, this transversal driving mechanism comprises transverse shifting servomotor 4, can drive mobile terminal 31 above microwell plate 1, move laterally (Y-direction namely in Fig. 1).
Described microwell plate 1 evenly has 8 × 12=96 micropore 5, longitudinal often row 12 micropores 5, laterally often arrange totally 8 micropores 5.Described photoelectric sensor 2 is photomultiplier, and this photomultiplier is laterally fixing.
The measuring method of chemical liquid luminescence intensity of the present invention, comprises the steps:
1) testing liquid is injected in the micropore 5 of the luminous measuring instrument microwell plate 1 of afore mentioned chemical;
2) the microwell plate drive system in the luminous measuring instrument of afore mentioned chemical and transversal driving mechanism is alternately started, "the" shape motion is done relative to microwell plate 1 in light transmitting fiber 3 mobile terminal 31, aim at each micropore 5 on microwell plate 1 one by one, when light transmitting fiber 3 mobile terminal 31 slips over each micropore, photoelectric sensor gathers photon count value.
2nd) when step light transmitting fiber mobile terminal 31 is moved relative to microwell plate 1, can as shown in Figure 2, back and forth movement by column; Also can as shown in Figure 3, back and forth movement line by line.
Claims (1)
1. a measuring method for chemical liquid luminescence intensity, adopts chemiluminescence measuring instrument, it is characterized in that:
Described chemiluminescence measuring instrument comprises microwell plate (1), photoelectric sensor (2) and microwell plate drive system, microwell plate drive system can drive microwell plate (1) to vertically move, it also comprises light transmitting fiber (3), this light transmitting fiber (3) one end is fixed on the input end of described photoelectric sensor (2), its other end is mobile terminal (31), is furnished with transversal driving mechanism, this transversal driving mechanism comprises transverse shifting servomotor (4), this transversal driving mechanism can drive mobile terminal (31) to move laterally in microwell plate (1) top,
Wherein microwell plate drive system is only equipped with longitudinal moving mechanism, and transverse shifting is driven by the transversal driving mechanism that volume is little;
Described microwell plate (1) evenly has 8 × 12=96 micropore (5), longitudinal often row 12 micropores (5), laterally often arrange totally 8 micropores (5);
Described photoelectric sensor (2) is end window type photomultiplier; Described photomultiplier is laterally fixing, and described photomultiplier is parallel to pedestal;
The measuring method of described chemical liquid luminescence intensity comprises the steps:
1) testing liquid is injected in the micropore (5) of described chemiluminescence measuring instrument microwell plate (1);
2) the microwell plate drive system in described chemiluminescence measuring instrument and transversal driving mechanism is alternately started, "the" shape motion is done relative to microwell plate (1) in light transmitting fiber (3) mobile terminal (31), aim at each micropore (5) on microwell plate (1) one by one, time not stopping continuously and slip over each micropore in light transmitting fiber (3) mobile terminal (31), photoelectric sensor gathers photon count value;
Wherein the 2nd) when step light transmitting fiber mobile terminal (31) is mobile relative to microwell plate (1), line by line or by column back and forth movement;
The measuring method of described chemical liquid luminescence intensity is all measured at round trip.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201110411503.4A CN102393358B (en) | 2011-12-12 | 2011-12-12 | The measuring method of chemical liquid luminescence intensity |
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| CN201110411503.4A CN102393358B (en) | 2011-12-12 | 2011-12-12 | The measuring method of chemical liquid luminescence intensity |
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| CN102393358A CN102393358A (en) | 2012-03-28 |
| CN102393358B true CN102393358B (en) | 2015-10-21 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104535760B (en) * | 2014-12-31 | 2016-08-24 | 重庆科斯迈生物科技有限公司 | Chemical illumination immunity analysis instrument detection case |
| CN106841178B (en) * | 2017-02-17 | 2019-10-18 | 郑州科蒂亚生物技术有限公司 | Chemiluminescence detection system and its microwell plate detect transmitting device |
| CN108519332B (en) * | 2018-04-28 | 2019-12-03 | 南通市肺科医院（南通市第六人民医院） | Full-automatic microwell plate system and the computer-readable medium for the system |
| CN109444118B (en) * | 2018-12-24 | 2021-07-27 | 三诺生物传感股份有限公司 | Measuring method and device for chemical luminous inspection instrument |
| CN113011549A (en) * | 2021-02-20 | 2021-06-22 | 重庆博奥新景医学科技有限公司 | Self-adaptive measurement and calculation method applied to luminescence value of chemiluminescence analyzer |
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| CN1311436A (en) * | 2000-03-01 | 2001-09-05 | 上海和泰光电科技有限公司 | Reading of biological chip fluorescent image on rotary platform |
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| CN101300478A (en) * | 2005-11-03 | 2008-11-05 | 霍夫曼-拉罗奇有限公司 | Analytical multi-spectral optical detection system |
| CN202393693U (en) * | 2011-12-12 | 2012-08-22 | 珠海丽珠试剂股份有限公司 | Chemiluminescent measuring instrument |
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| US5459325A (en) * | 1994-07-19 | 1995-10-17 | Molecular Dynamics, Inc. | High-speed fluorescence scanner |
| CN1311436A (en) * | 2000-03-01 | 2001-09-05 | 上海和泰光电科技有限公司 | Reading of biological chip fluorescent image on rotary platform |
| CN1521497A (en) * | 2003-02-14 | 2004-08-18 | ��������Ƽ�ʵҵ�ɷ�����˾ | Microarray biochip reflective image obtaining and analysis device and method |
| CN101300478A (en) * | 2005-11-03 | 2008-11-05 | 霍夫曼-拉罗奇有限公司 | Analytical multi-spectral optical detection system |
| CN202393693U (en) * | 2011-12-12 | 2012-08-22 | 珠海丽珠试剂股份有限公司 | Chemiluminescent measuring instrument |
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