CN102393358A - Chemiluminescence measuring instrument and measuring method of chemical liquid luminescence intensity - Google Patents
Chemiluminescence measuring instrument and measuring method of chemical liquid luminescence intensity Download PDFInfo
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- CN102393358A CN102393358A CN2011104115034A CN201110411503A CN102393358A CN 102393358 A CN102393358 A CN 102393358A CN 2011104115034 A CN2011104115034 A CN 2011104115034A CN 201110411503 A CN201110411503 A CN 201110411503A CN 102393358 A CN102393358 A CN 102393358A
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- microwell plate
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- chemiluminescence
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Abstract
The invention relates to a chemiluminescence measuring instrument and a measuring method of chemical liquid luminescence intensity. Current chemiluminescence measuring instruments have a large volume and a slow measuring speed. Therefore, the chemiluminescence measuring instrument comprises a micropore plate, a photoelectric sensor, and a micropore plate driving system; the micropore plate driving system can drive the micropore plate to move longitudinally; the chemiluminescence measuring instrument is characterized in that the instrument also comprises an optical fiber; one end of the the optical fiber is fixed on an input end of the photoelectric sensor, and the other end is a movable end, and is equipped with a transverse driving mechanism; the transverse driving mechanism can drive the movable end to move laterally above the micropore plate. For optimization, when the movable end of the optical fiber moves relative to the micropore plate, row-by-row reciprocating motion or column-by-column reciprocating motion is performed. The chemiluminescence measuring instrument of the invention has the advantages of small volume, high measuring speed, and high efficiency, and the measuring method of chemical liquid luminescence intensity of the invention measures during the reciprocating stroke, has a high speed, and high efficiency, and is applicable to the fields of biochemistry and clinical diagnosis.
Description
Technical field
The present invention relates to the measuring method of a kind of chemiluminescence measuring instrument and chemical liquid luminous intensity.
Background technology
The chemiluminescence measurement is to utilize instrument to the catching and analyzing of faint optical signal, and is widely used in biological chemistry and clinical diagnosis field at present.Instrument is an optics, machinery, and electronics is controlled the product that multi-field technology combines automatically.
Photomultiplier output signal has into the characteristics of nature discretize under the low light level, and when the low light level shone the photomultiplier transit tube cathode, each incident photon made electronics of photocathode emission with certain probability (being quantum efficiency).This photoelectron forms a current impulse at last through the multiplication of dynode system in plate tank, form a potential pulse through pull-up resistor, and this pulse is called single photon pulses.According to nonoverlapping photonic energy pulse of calculating gained, can know the luminous intensity of fluid to be measured.
Existing chemiluminescence measuring instrument all is through mobile microwell plate, pauses by the hole and measures testing liquid, and measuring speed is slow, and its photomultiplier is perpendicular to pedestal, and volume is big.
Summary of the invention
The technical matters that the present invention will solve is how to overcome the existing defective that chemiluminescence measuring instrument volume is big, measuring speed is slow, and the measuring method of a kind of chemiluminescence measuring instrument and chemical liquid luminous intensity is provided.
For solving the problems of the technologies described above; This chemiluminescence measuring instrument comprises microwell plate, photoelectric sensor and microwell plate drive system, and the microwell plate drive system can drive microwell plate and vertically move, and it is characterized in that: it also comprises light transmitting fiber; This light transmitting fiber one end is fixed on the input end of said photoelectric sensor; Its other end is the mobile terminal, is furnished with transversal driving mechanism, and this transversal driving mechanism can drive the mobile terminal and above microwell plate, move laterally.So design, the microwell plate drive system only is equipped with longitudinal moving mechanism, laterally moves by the little transversal driving mechanism of volume to drive, and the horizontal shift motion of microwell plate reduces, whole long-pending the reducing of instrument, measuring speed is accelerated greatly.
As optimization, evenly have 8 * 12=96 micropore on the said microwell plate, 12 micropores of vertical every row, horizontal every row is totally 8 micropores.
As optimization, said photoelectric sensor is end window type photomultiplier.
As optimization, said photomultiplier crosswise fixed.So design, said photomultiplier is parallel to pedestal, and volume is littler.
The measuring method of chemical liquid luminous intensity of the present invention comprises the steps:
1) testing liquid is injected in the micropore of aforementioned chemiluminescence measuring instrument microwell plate;
2) alternately start microwell plate drive system and transversal driving mechanism in the aforementioned chemiluminescence measuring instrument; The motion of " it " font is done with respect to microwell plate in the light transmitting fiber mobile terminal; Do not stop when slipping over each micropore continuously in the light transmitting fiber mobile terminal, photoelectric sensor is gathered photon count value.When existing chemiluminescence measuring instrument was measured testing liquid, photoelectric sensor and microwell plate relatively moved, and needed the pause of every hole is measured.So design, when photoelectric sensor of the present invention and microwell plate relatively move, the continuous coverage that do not stop, efficient is higher.
When as optimization, the 2nd) step, moved with respect to microwell plate the light transmitting fiber mobile terminal, back and forth movement line by line.
As optimization, the 2nd) when the step, moved with respect to microwell plate the light transmitting fiber mobile terminal, by the row back and forth movement.
It is little that chemiluminescence measuring instrument of the present invention has a volume, the advantage that measuring speed is fast, efficient is high, and the measuring method of chemical liquid luminous intensity of the present invention is all measured at round trip, and speed is fast, efficient is high, is applicable to biological chemistry and clinical diagnosis field.
Description of drawings
Be described further below in conjunction with the measuring method of accompanying drawing chemiluminescence measuring instrument of the present invention and chemical liquid luminous intensity:
Fig. 1 is a chemiluminescence measuring instrument perspective view of the present invention;
Fig. 2 is a kind of the move towards synoptic diagram of the measuring method light transmitting fiber mobile terminal of this chemical liquid luminous intensity with respect to microwell plate;
Fig. 3 is that synoptic diagram is moved towards with respect to the another kind of microwell plate in the measuring method light transmitting fiber mobile terminal of this chemical liquid luminous intensity.
Among the figure: 1 is that microwell plate, 2 is that photoelectric sensor, 3 is that light transmitting fiber, 31 is that mobile terminal, 4 is that horizontal shift servo motor, 5 is micropore.
Embodiment
Embodiment one: shown in Fig. 1-3; This chemiluminescence measuring instrument comprises microwell plate 1, photoelectric sensor 2 and microwell plate drive system; The microwell plate drive system can drive microwell plate 1 and vertically move (being the directions X among Fig. 1), and it is characterized in that: it also comprises light transmitting fiber 3, and these light transmitting fiber 3 one ends are fixed on the input end of said photoelectric sensor 2; Its other end is mobile terminal 31; Be furnished with transversal driving mechanism, this transversal driving mechanism comprises horizontal shift servo motor 4, can drive mobile terminal 31 above microwell plate 1, move laterally (being the Y direction among Fig. 1).
Evenly have 8 * 12=96 micropore 5 on the said microwell plate 1,12 micropores 5 of vertical every row, horizontal every row is totally 8 micropores 5.Said photoelectric sensor 2 is a photomultiplier, this photomultiplier crosswise fixed.
The measuring method of chemical liquid luminous intensity of the present invention comprises the steps:
1) testing liquid is injected in the micropore 5 of aforementioned chemiluminescence measuring instrument microwell plate 1;
2) alternately start microwell plate drive system and transversal driving mechanism in the aforementioned chemiluminescence measuring instrument; The motion of " it " font is done with respect to microwell plate 1 in light transmitting fiber 3 mobile terminals 31; Aim at each micropore 5 on the microwell plate 1 one by one; When light transmitting fiber 3 mobile terminals 31 slipped over each micropore, photoelectric sensor was gathered photon count value.
When the 2nd) step, moved with respect to microwell plate 1 light transmitting fiber mobile terminal 31, can be as shown in Figure 2, by the row back and forth movement; Also can be as shown in Figure 3, back and forth movement line by line.
Claims (7)
1. chemiluminescence measuring instrument; Comprise microwell plate, photoelectric sensor and microwell plate drive system, the microwell plate drive system can drive microwell plate and vertically move, and it is characterized in that: it also comprises light transmitting fiber; This light transmitting fiber one end is fixed on the input end of said photoelectric sensor; Its other end is the mobile terminal, is furnished with transversal driving mechanism, and this transversal driving mechanism can drive the mobile terminal and above microwell plate, move laterally.
2. chemiluminescence measuring instrument according to claim 1 is characterized in that: evenly have 8 * 12=96 micropore on the said microwell plate, and 12 micropores of vertical every row, horizontal every row is totally 8 micropores.
3. chemiluminescence measuring instrument according to claim 1 and 2 is characterized in that: said photoelectric sensor is end window type photomultiplier.
4. chemiluminescence measuring instrument according to claim 3 is characterized in that: said photomultiplier crosswise fixed.
5. the measuring method of a chemical liquid luminous intensity comprises the steps:
1) testing liquid is injected in the micropore of the said chemiluminescence measuring instrument of claim 1 microwell plate;
2) alternately start microwell plate drive system and transversal driving mechanism in the said chemiluminescence measuring instrument of claim 1; The motion of " it " font is done with respect to microwell plate in the light transmitting fiber mobile terminal; Do not stop when slipping over each micropore continuously in the light transmitting fiber mobile terminal, photoelectric sensor is gathered photon count value.
6. the measuring method of chemical liquid luminous intensity according to claim 5 is characterized in that: when the 2nd) step, moved with respect to microwell plate the light transmitting fiber mobile terminal, and back and forth movement line by line.
7. the measuring method of chemical liquid luminous intensity according to claim 5 is characterized in that: when the 2nd) step, moved with respect to microwell plate the light transmitting fiber mobile terminal, by the row back and forth movement.
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
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CN104535760A (en) * | 2014-12-31 | 2015-04-22 | 重庆科斯迈生物科技有限公司 | Detecting box of chemiluminescence immunity analyzer |
CN106841178A (en) * | 2017-02-17 | 2017-06-13 | 郑州艾斯亚生物科技有限公司 | Chemiluminescence detection system and its microwell plate detection transmitting device |
CN108519332A (en) * | 2018-04-28 | 2018-09-11 | 南通市肺科医院(南通市第六人民医院) | Full-automatic microwell plate system and the computer-readable medium for the system |
CN109444118A (en) * | 2018-12-24 | 2019-03-08 | 三诺生物传感股份有限公司 | Chemiluminescence assay apparatus measures method and device |
WO2022174511A1 (en) * | 2021-02-20 | 2022-08-25 | 重庆博奥新景医学科技有限公司 | Adaptive measurement and calculation method applied to luminescence value of chemiluminescence analyzer |
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Publication number | Priority date | Publication date | Assignee | Title |
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CN104535760A (en) * | 2014-12-31 | 2015-04-22 | 重庆科斯迈生物科技有限公司 | Detecting box of chemiluminescence immunity analyzer |
CN106841178A (en) * | 2017-02-17 | 2017-06-13 | 郑州艾斯亚生物科技有限公司 | Chemiluminescence detection system and its microwell plate detection transmitting device |
CN106841178B (en) * | 2017-02-17 | 2019-10-18 | 郑州科蒂亚生物技术有限公司 | Chemiluminescence detection system and its microwell plate detect transmitting device |
CN108519332A (en) * | 2018-04-28 | 2018-09-11 | 南通市肺科医院(南通市第六人民医院) | Full-automatic microwell plate system and the computer-readable medium for the system |
CN108519332B (en) * | 2018-04-28 | 2019-12-03 | 南通市肺科医院(南通市第六人民医院) | Full-automatic microwell plate system and the computer-readable medium for the system |
CN109444118A (en) * | 2018-12-24 | 2019-03-08 | 三诺生物传感股份有限公司 | Chemiluminescence assay apparatus measures method and device |
CN109444118B (en) * | 2018-12-24 | 2021-07-27 | 三诺生物传感股份有限公司 | Measuring method and device for chemical luminous inspection instrument |
WO2022174511A1 (en) * | 2021-02-20 | 2022-08-25 | 重庆博奥新景医学科技有限公司 | Adaptive measurement and calculation method applied to luminescence value of chemiluminescence analyzer |
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