CN102250753A - Device for drawing deoxyribonucleic acid (DNA) molecule into line shape and application thereof - Google Patents
Device for drawing deoxyribonucleic acid (DNA) molecule into line shape and application thereof Download PDFInfo
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- CN102250753A CN102250753A CN2011101375167A CN201110137516A CN102250753A CN 102250753 A CN102250753 A CN 102250753A CN 2011101375167 A CN2011101375167 A CN 2011101375167A CN 201110137516 A CN201110137516 A CN 201110137516A CN 102250753 A CN102250753 A CN 102250753A
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- sheet glass
- sheet
- dna
- leading screw
- glass sheet
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Abstract
The invention discloses a device for drawing a deoxyribonucleic acid (DNA) molecule into a line shape and application thereof. The device disclosed by the invention comprises at least one pair of glass sheets for clamping the DNA molecule, wherein each pair of glass sheets comprise a first glass sheet for placing the DNA molecule and a second glass sheet for covering the first glass sheet; a space for accommodating the DNA molecule is formed between the first glass sheet and the second glass sheet; and the first glass sheet is a silylated glass sheet. The device also comprises a power mechanism for dragging the second glass sheet. The device used for preparing microscopic examination sample slices has the following advantages: 1. the speed is uniform and can be maintained at a set speed all along; 2. the direction is consistent, thereby guaranteeing that the drawn DNA is a straight line; 3. shaking is reduced to the minimum, so that the DNA fracture caused by shaking is maximally reduced; 4. the experiment consistency is guaranteed, multiple sample slices can be simultaneously drawn, and the consistency of the experiments on different sample slices is guaranteed; and 5. the device is simple to operate and agile to use, and the slice drawing speed can be randomly set.
Description
Technical field
The present invention relates to a kind of device and application thereof that dna molecular is pulled into wire
Background technology
The way of traditional similar experiment be sheet glass as on the inclined-plane, allow the solution droplets that contains DNA on the inclined-plane, trickle, utilize stringy shearing force that DNA is spread upon on the sheet glass.This way effect, efficient are all poor, and the repeatability of experimental result is also poor.
Summary of the invention
The object of the present invention is to provide a kind of device that dna molecular is pulled into wire.
Device provided by the invention comprises the sheet glass of the described dna molecular of at least one pair of clamping; Every pair of sheet glass includes: first sheet glass and of placing dna molecular covers second sheet glass of described first sheet glass; Form the accommodation space of described dna molecular between described first sheet glass and second sheet glass; Described first sheet glass is the sheet glass of silanization; Said apparatus also comprises the actuating unit of described second sheet glass of tractive.
Above-mentioned actuating unit comprises motor and the leading screw that connects by shaft coupling, and described leading screw is connected with described second sheet glass; Described motor drives described second sheet glass by described leading screw and moves axially along leading screw.
In another embodiment, said apparatus also comprises the buffer gear that is arranged between described actuating unit and described second sheet glass.
This is stated actuating unit and comprises motor and the leading screw that connects by shaft coupling, and described leading screw is connected with described second sheet glass by described buffer gear.
Above-mentioned buffer gear can be a vibration damping sheet.
Above-mentioned buffer gear also can be interconnective line slideway and vibration damping sheet, and described line slideway is connected with described leading screw, and described vibration damping sheet is connected with described second sheet glass.
Above-mentioned line slideway comprises 2 guide rails and 1 slide block, and the relative side of 2 guide rails has guide groove, and the two ends of slide block put in respectively in the guide groove of 2 guide rails, realizes being slidingly matched with 2 intravital guide grooves of guide rail.
Preferably, the ball row of carrying usefulness are set respectively at the two ends of slide block, slide block is listed in 2 intravital guide grooves of guide rail by ball and moves.
A nearlyer step says that above-mentioned vibration-damping sheet is an elastic material, is preferably hardboard paper or plastic sheet.
Above-mentioned device also belongs to protection scope of the present invention in the application that dna molecular is pulled in the wire.
Above-mentioned dna molecular is to be dissolved in the MES damping fluid of pH5.5.During application, the translational speed of second sheet glass in the above-mentioned device is a 1-1000 millimeter per second.
Use this device to manufacture the advantage of microscope inspection print with respect to the traditional-handwork film-making:
1, speed is even, can remain setting speed;
2, direction unanimity guarantees that the DNA that pulls out is a straight line;
3, vibrations are reduced to minimum, buffer gear (line slideway and/or vibration damping sheet) is arranged, can reduce the dna break that vibrations cause to greatest extent;
4, guarantee that experiment is consistent, can draw many prints simultaneously, guarantee the consistence between the different prints of experiment;
5, simple to operate, to use flexibly, pulling-on piece speed can arbitrarily be set.
Using the dna molecular of microscopic examination wire, is a kind of effective ways of checking the dna replication dna changing conditions from the molecule rank.Along with the continuous upgrading of biological study content in the recent both at home and abroad academic and industrial circle and laboratory facilities, the demand of this type of experiment is grown with each passing day.This experimental installation has a good application prospect.
Description of drawings
Fig. 1 is the structural representation of embodiment 1.
Fig. 2 is a DNA metamorphosis photo among the embodiment 2.
Embodiment
The invention will be further described below in conjunction with specific embodiment, but the present invention is not limited to following examples.
Among the following embodiment, if no special instructions, be ordinary method.
Embodiment 1, dna molecular pulled into the device of wire
As shown in Figure 1, provided by the invention dna molecular is pulled into the device of wire, comprise actuating unit 1, buffer gear 2 and topworks 3.Wherein, topworks 3 is the sheet glass of a pair of clamping dna molecular, is specifically as follows the cover glass 31 and the slide glass 32 that stack up and down, and wherein slide glass 32 is to handle through silanization.Form the accommodation space of dna molecular between cover glass 31 and the slide glass 32.Actuating unit 1 comprises motor 11 and the leading screw 12 that connects by shaft coupling, and motor 11 and leading screw 12 are coaxial.Buffer gear 2 comprises interconnective line slideway 21 and vibration damping sheet 22, line slideway 21 can adopt any type of guide rail in the prior art, the line slideway 21 that present embodiment adopts comprises 211,212 and 1 slide blocks 213 of 2 guide rails, the relative side of guide rail 211,212 has guide groove, the two ends of slide block 213 put in respectively in the guide groove of guide rail 211,212, realize being slidingly matched with the guide groove of guide rail 211,212.The ball row of carrying usefulness can certainly be set respectively at the two ends of slide block 213, and slide block 213 is listed in by ball in the guide groove of guide rail 211,212 and moves.
Leading screw 12 is connected with vibration damping sheet 22 by slide block 213, and vibration damping sheet 22 is connected with cover glass 31.Vibration damping sheet 22 in the present embodiment is a hardboard paper.
In the present embodiment, motor can be servomotor, and leading screw can be ball-screw.Device operational process of the present invention is as follows: start servomotor 11, thereby motor 11 drives ball-screw 12 rotations and realizes that the horizontal axis of ball-screw 12 moves, line slideway 21 and vibration damping sheet 22 are also corresponding to be moved, thereby realizes that cover glass 31 relative slide glasss 32 are moved.
In other embodiments, the actuating unit 1 in apparatus of the present invention can directly be connected with topworks 3, so buffer gear 2 can omit; Certainly, the buffer gear 2 in apparatus of the present invention also can omit a part, as removing line slideway 21 or removing vibration damping sheet 22; During actual production, line slideway 21 and leading screw 12 can be become one, thereby a guide rail needn't be set separately or can replace vibration damping sheet 22 in slide block 213 terminal projections of attaching " possessing suitable stiff and elasticity "; According to the experiment needs, also topworks 3 can be set to the topworks more than 2 pairs.
The above only is preferred embodiment of the present invention, and is in order to restriction the present invention, within the spirit and principles in the present invention not all, any modification of being done, is equal to replacement, improvement etc., all should be included within protection scope of the present invention.
DNA is dissolved in the MES damping fluid of pH 5.5, drops on the slide glass 32, covered 31 is started motor 11, and leading screw 12 drives line slideway 21, hardboard paper 22 horizontal axis move, thereby pulling cover glass 31 pulls into wire with dna molecular.In cover glass 31 drawing process, the photo of DNA change of shape is shown in A, B, C and the D of Fig. 2.
The principle of the invention: DNA is dissolved in the MES damping fluid of pH 5.5, the DNA end is exposed intramolecular hydrophobic group by partially denaturing.Hydrophobic interaction takes place in hydrophobic group and silanized glass surface, makes DNA one end fixedly on the slide glass.Along continuous straight runs is (1-1000 micron per second) pulling cover glass slowly at the uniform velocity, and cover glass pulling liquid level is to a lateral movement, and the surface tension when utilizing the liquid level pulling between water and the air phase pulls into wire with DNA.Examine under a microscope the linear DNA molecule of glass sheet surface, can obtain intuitively changing relevant various biology conclusions with dna molecular.
Claims (10)
1. one kind pulls into the device of wire with dna molecular, and it is characterized in that: it comprises the sheet glass of the described dna molecular of at least one pair of clamping; Every pair of sheet glass includes: first sheet glass and of placing dna molecular covers second sheet glass of described first sheet glass; Form the accommodation space of described dna molecular between described first sheet glass and second sheet glass; Described first sheet glass is the sheet glass of silanization; Described device also comprises the actuating unit of described second sheet glass of tractive.
2. device as claimed in claim 1 is characterized in that: described actuating unit comprises motor and the leading screw that connects by shaft coupling, and described leading screw is connected with described second sheet glass; Described motor drives described second sheet glass by described leading screw and moves axially along leading screw.
3. device as claimed in claim 1 is characterized in that: described device also comprises the buffer gear that is arranged between described actuating unit and described second sheet glass.
4. device as claimed in claim 3 is characterized in that: described actuating unit comprises motor and the leading screw that connects by shaft coupling, and described leading screw is connected with described second sheet glass by described buffer gear.
5. as claim 3 or 4 described devices, it is characterized in that: described buffer gear is a vibration damping sheet.
6. as claim 3 or 4 described devices, it is characterized in that: described buffer gear is interconnective line slideway and vibration damping sheet, and described line slideway is connected with described leading screw, and described vibration damping sheet is connected with described second sheet glass.
7. as claim 5 or 6 described devices, it is characterized in that: described vibration-damping sheet is an elastic material, is preferably hardboard paper or plastic sheet.
Among the claim 1-7 arbitrary described device in the application that dna molecular is pulled in the wire.
9. application as claimed in claim 8 is characterized in that: described dna molecular is to be dissolved in the MES damping fluid of pH5.5.
10. use as claimed in claim 8 or 9, it is characterized in that: the translational speed of second sheet glass among the claim 1-7 in arbitrary described device is a 1-1000 millimeter per second.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110137516.7A CN102250753B (en) | 2011-05-25 | 2011-05-25 | Device for drawing deoxyribonucleic acid (DNA) molecule into line shape and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110137516.7A CN102250753B (en) | 2011-05-25 | 2011-05-25 | Device for drawing deoxyribonucleic acid (DNA) molecule into line shape and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102250753A true CN102250753A (en) | 2011-11-23 |
| CN102250753B CN102250753B (en) | 2014-02-05 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110137516.7A Expired - Fee Related CN102250753B (en) | 2011-05-25 | 2011-05-25 | Device for drawing deoxyribonucleic acid (DNA) molecule into line shape and application thereof |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004125601A (en) * | 2002-10-02 | 2004-04-22 | National Food Research Institute | Method for elongating and immobilizing dna |
| CN101251866A (en) * | 2008-04-10 | 2008-08-27 | 中国科学院上海药物研究所 | Device and method for optimizing stretching direction in stretching molecular kinetics simulation |
| US20100144020A1 (en) * | 2008-12-08 | 2010-06-10 | Electronics And Telecommunications Research Institute | Disposable diagnostic kit |
| CN201610428U (en) * | 2009-12-16 | 2010-10-20 | 重庆大学 | Cell stretching device with continuously-adjustable strain |
| US20110111487A1 (en) * | 2001-09-13 | 2011-05-12 | Axela Biosensors Inc. | Mehtod and apparatus for assay based on light diffraction |
-
2011
- 2011-05-25 CN CN201110137516.7A patent/CN102250753B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110111487A1 (en) * | 2001-09-13 | 2011-05-12 | Axela Biosensors Inc. | Mehtod and apparatus for assay based on light diffraction |
| JP2004125601A (en) * | 2002-10-02 | 2004-04-22 | National Food Research Institute | Method for elongating and immobilizing dna |
| CN101251866A (en) * | 2008-04-10 | 2008-08-27 | 中国科学院上海药物研究所 | Device and method for optimizing stretching direction in stretching molecular kinetics simulation |
| US20100144020A1 (en) * | 2008-12-08 | 2010-06-10 | Electronics And Telecommunications Research Institute | Disposable diagnostic kit |
| CN201610428U (en) * | 2009-12-16 | 2010-10-20 | 重庆大学 | Cell stretching device with continuously-adjustable strain |
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| CN102250753B (en) | 2014-02-05 |
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