CN102199531A - Microfluidic chip for multiple loop-mediated isothermal amplification (LAMP) detection and preparation method thereof - Google Patents
Microfluidic chip for multiple loop-mediated isothermal amplification (LAMP) detection and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of nucleic acid isothermal amplification, and particularly relates to a microfluidic chip for multiple loop-mediated isothermal amplification (LAMP) detection and a preparation method thereof. The chip is made of a high polymer, and is prepared by a micro-electromechanical system (MEMS) method. The chip mainly comprises amplification pools which are spatially and sequentially arranged, capillary channels, and connecting pipelines, wherein the amplification pools realize the effective discrimination of multiple LAMP signals through the spatial discrimination of the signals; the capillary channels are used for preventing the intersection and mixing of LAMP primers, amplification products, byproducts and the like among different amplification pools; and the amplification pools and the capillary channels are communicated through the connecting pipelines, so that fluid steadily and uniformly flows into the amplification pools from the capillary channels. The chip is easy to manufacture and operate; and an effective scheme is provided for the realization of the synchronous clinical detection of various pathogens by an LAMP method.
Description
Technical field
The invention belongs to nucleic acid isothermal amplification technique field, be specifically related to a kind of micro-fluidic chip that is used for multiple LAMP detection and its production and use.
Technical background
The isothermal nucleic acid amplification is relative polymerase chain reaction (Polymerase chain reaction, PCR) an alternating temperature nucleic acid amplification.This technology can not rely on expensive instruments such as temperature cycler, and amplification of nucleic acid in a large number just under steady temperature is subjected to the great attention of academia and industrial community in recent years, develops polytype isothermal nucleic acid amplification in succession.Ring mediated isothermal nucleic acid amplification (Loop-mediated isothermal amplification, LAMP) the earliest by Japanese scientist's invention, this technology has highly sensitive, specificity is good, speed of response is fast, and reaction result is advantage such as naked eyes judgement directly, through nearly 10 years academic research and clinical verification, obtained nearly 400 items and closed patent, and come into the market with the form of various detection kit.Detection when " the isothermal pattern " but of LAMP reaction and its complicated nucleic acid probe make it can not realize in the sample plurality of target thing, promptly multiple detection (promptly detecting plurality of target thing in the sample simultaneously).The developing into us a highly effective solution be provided of micro-fluidic chip technology.The present invention utilizes the micro-fluidic chip correlation technique successfully to capture the multiplicity difficult problem that LAMP detects under the support of related item.
Summary of the invention
The purpose of this invention is to provide a kind of micro-fluidic chip that can be used for multiple LAMP detection and its production and use.
The micro-fluidic chip that is used for multiple LAMP detection provided by the invention structurally mainly comprises three parts: amplification pond, connecting tube and capillary channel.See shown in the accompanying drawing 1.Bag is by last specific probe at target compound in the amplification pond.The amplification pond can be arranged by the various forms spacial ordering, as array-like or divergent shape etc.The Demand Design that its quantity can detect according to target compound, as triple, five is heavy or ten heavy or the like, unrestricted.Effective differentiation of multiple LAMP signal is assigned to realize in the series amplification pond that spacial ordering is arranged by the space region of signal.Capillary channel mainly is the specific nucleic acid probe and reaction product crossed contamination in different ponds of each amplification of characteristic limitations in pond that utilizes its physics confinement (inferior quality transmission coefficient), be that small-scale structure capillaceous makes fluid wherein have very little spread coefficient, can effectively stop the LAMP primer, amplified production and by product (as magnesium pyrophosphate etc.) the mutual intersection between difference amplification pond is mixed.Simultaneously also utilize pulling force capillaceous to be convenient to the sample introduction of sample and reaction solution.Be communicated with connecting tube between amplification pond and the kapillary, make fluid steadily flow into the amplification pond from capillary channel uniformly, and avoid forming in the reaction process steam bubble.
The present invention also provides the preparation method of above-mentioned micro-fluidic chip, and concrete steps are as follows:
1) making of chip template: utilize the method for traditional MEMS to make the chip template.
2) chip cast, the degassing is solidified: after dimethyl siloxane and solidifying agent are mixed, be poured on the chip template vacuum outgas, hot setting.
3) chip punching, involution: punch at chip sample introduction place with syringe needle, after plasma treatment, with the permanent bonding of slide.
4) functionalization chip: the probe of tested target set nucleic acid thing correspondence is passed through injection-method of evaporation bag quilt in the amplification pond of chip correspondence, finish the functionalization chip.
The inventive method can be made all kinds multiple LAMP detection chip of polymkeric substance of (comprising the chip size size, arrangement of amplification pond and number etc.).And can finish various functional chips very easily, and do not produce crossed contamination by injection-method of evaporation.
The checking of micro-fluidic chip of the present invention:
The present invention utilizes the LAMP amplification system that the validity of chip is verified.In ten channel microfluidic chips, the 1st, 2, bag or not by nucleic acid probe in the 5th amplification pond by last specific probe at four kinds of target set nucleic acid things (Probe 1, Probe 2, Probe 3, Probe 4) in 3, the 4 amplification ponds.Feed sample (containing four kinds of target nucleic acids), feed the LAMP reaction solution again, LAMP reacts 30 min-60 min under constant temperature.(d) green fluorescence LAMP signal (c) white precipitate appearred, in experimental result such as accompanying drawing 2 in being coated with the amplification pond of target compound probe; Distinctive LAMP scalariform amplified band (e) has also appearred in the agarose gel electrophoresis.And the LAMP signal does not appear in not wrapping by the amplification pond of nucleic acid probe, amplified band does not appear in electrophoretic analysis.
Use the basic operational steps of micro-fluidic chip of the present invention as follows:
1) sample injects: with liquid-transfering gun test sample is added to the chip addition pool, adds a certain amount of LAMP amplification liquid then.Under pulling force capillaceous, sample and amplification liquid distribute equably rapidly and enter the augmentation detection pond.
2) constant-temperature amplification: with the chip involution, in water-bath, carry out the about 30 min-60 min of isothermal reaction with uncured polydimethylsiloxane.
3) result judges: can directly whether produce and judge detected result according to white precipitate in the amplification pond, also can be under ultraviolet lamp basis whether produce fluorescence and come result of determination.
Compared with prior art, the present invention has following advantage:
1) the present invention is by the different effective differentiations that realize multiple LAMP signal in amplification pool space position on the chip, visual result, and effect is obvious, need not be very beneficial for the promotion and application of LAMP method in basic unit by other expensive instruments.
2) by MEMS chip manufacturing method, pond spatial disposition arbitrarily on chip as required can realize increasing.
3) have unlimited extensibility on this chip structure pattern theory, can finish according to the actual requirements arbitrarily that multiple LAMP effectively increases, thereby satisfy effective quick diagnosis of clinical any multiple pathogenic agent.
4) judgement is no more than 1h from the application of sample to result, and operation is very simple and convenient, and the non-specialised staff just can successfully operate by simple demonstration.
Description of drawings
The microfluidic chip structure of the multiple LAMP of Fig. 1.
Fig. 2 is used for the compliance test result of the micro-fluidic chip of multiple LAMP.
The multiple LAMP chip fast typing of three kinds of influenza viruses of Fig. 3 detects.
The multiple LAMP chip somatotype of eight kinds of important swine disease pathogenic agent of Fig. 4 detects.
Embodiment
Below in conjunction with specific examples the present invention is described further, but specific examples is not done any qualification to the present invention.
Embodiment 1Influenza A virus (Flu A virus), seasonal influenza A virus (Seasonal flu A virus) and pig source property influenza A virus (Pandemic flu A virus) fast typing in 2009 detect.
Chip specific design size: the amplification pond be of a size of (long: 10 mm, wide: 0.6 mm, dark: 0.8 mm); Capillary channel be of a size of (long: 2 mm, wide: 0.1 mm, dark: 0.1 mm).
The specificity LAMP probes of three kinds of influenza viruses is wrapped respectively by in the 1-the 4 amplification pond 1 (1 '), 2 (2 '), 3 (3 '); Amplification pond 4 (4 ') bag is by last positive control LAMP probe; The 5th amplification pond 5 (5 ') is dressing probe not, as blank.We detect three kinds of important influenza viruses of somatotype with this functional chip.
Get three kinds of viral standard substance liquid of 4 μ L and clinical reference material respectively, be added to the chip well, add 46 μ L LAMP amplification liquid then, the sealing chip puts 65
oC water-bath 1h.
Result such as accompanying drawing 3 and subordinate list 1:
For three kinds of standard substance, LAMP signal (white precipitate, a-f, or green fluorescence a '-f ') appears in the pond of increasing accordingly, and signal does not appear in other amplification ponds.
For three kinds of clinical reference materials, the gained result also conforms to expectation, first stream virus control 2 (2 '), 4 (4 ') amplifications pond signal, seasonal first stream virus control 1 (1 '), 2 (2 ') and 4 (4 ') amplifications pond signal, pig source property first stream virus control 2 (2 '), 3 (3 ') and 4 (4 ') the pond signal that increases.The result meets expectation, and the nucleotide sequence of people's gene group appears containing in the amplified signal interpret sample in 4 (4 ') amplifications pond.
Simultaneously we are the detection of this chip application in 8 strain clinical samples, detected result such as subordinate list 1.It is pig source property influenza virus that sample 1,2,3,4 detected results demonstrate sample, and sample 5,6 may contain three kinds of influenza viruses simultaneously, and sample 7,8 does not contain this three kinds of influenza viruses, and detected result detects with hospital substantially and conforms to.
All test sample, signal does not appear in the negative amplification of chip Chi Zhongdou, illustrates that there is not signal hybridization phenomenon in different amplifications pond, and detected result is reliable.
The concrete size of chip is same as described above, wrap respectively by last at Schweineseuche virus (FMDV) in the multiple chip amplification of LAMP pond 1-8, Pestivirus suis (CSFV), PRRS virus (PRRSV), transmissible gastro-enteritis virus (TGEV), PRV (Pseudorabies virus) (PRV), pig parvoviral (PPV), pig circular ring virus (PCV), the LAMP specific nucleic acid probe of eight kinds of important swine disease viruses of swine influenza virus (SIV), the function typing chip of eight kinds of swine disease viruses of formation; No. 9 amplification Chi Bao are by last other probes, as negative control; No. 10 amplification pond dressing probes not are as blank.
In the chip addition pool, add sample 1 (FMDV) respectively, sample 2 (CSFV), sample 3(PRRSV), sample 4 (TGEV), sample 5 (PRV), sample 6 (PPV), sample 7 (PCV), sample 8(SIV), sample 9(FMDV, CSFV), sample 10(FMDV, CSFV, PRRSV), sample 11(FMDV, CSFV, PRRSV, TGEV), sample 12(FMDV, CSFV, PRRSV, TGEV, PRV), sample 13(FMDV, CSFV, PRRSV, TGEV, PRV, PPV), sample 14(FMDV, CSFV, PRRSV, TGEV, PRV, PPV, PCV), sample 15(FMDV, CSFV, PRRSV, TGEV, PRV, PPV, PCV, SIV), add LAMP amplification liquid then, the sealing chip puts 65
oC water-bath 1h.
Experimental result such as accompanying drawing 4 (a-o) according to the result, illustrate that the multiple chip of this LAMP can distinguish eight kinds of swine disease viruses well, also can determine the type of target pathogenic agent in the sample.
The multiple LAMP chip of table 1 is to three kinds of influenza virus fast typing detected results
Claims (3)
1. one kind is used for the micro-fluidic chip that multiple LAMP detects, and it is characterized in that being made of three essential parts: amplification pond, capillary channel and connecting tube; Wherein, described amplification pond is that spacial ordering is arranged, and bag is by last specific probe at target compound in the amplification pond, and effective differentiation of multiple LAMP signal is assigned to realize in the amplification pond by the space region of signal; Described capillary channel is used for limiting the specific nucleic acid probe and the crossed contamination of reaction product in different ponds in each amplification pond, also is used for the sample introduction of sample and reaction solution simultaneously; Described connecting tube is used for being communicated with amplification pond and capillary channel, makes fluid steadily flow into the amplification pond from capillary channel uniformly, and avoids forming in the reaction process steam bubble.
2. the preparation method of micro-fluidic chip as claimed in claim 1 is characterized in that concrete steps are:
1) making of chip template: utilize the MEMS method to make the chip template;
2) chip cast, the degassing is solidified: after dimethyl siloxane and solidifying agent are mixed, be poured on the chip template vacuum outgas, hot setting;
3) chip punching, involution: punch at chip sample introduction place with syringe needle, after plasma treatment, with the permanent bonding of slide;
4) functionalization chip: the probe of tested target set nucleic acid thing correspondence is passed through injection-method of evaporation bag quilt in the amplification pond of chip correspondence, finish the functionalization chip.
3. the using method of micro-fluidic chip as claimed in claim 1 is characterized in that concrete steps are:
1) sample injects: with liquid-transfering gun test sample is added to the chip addition pool, adds LAMP amplification liquid then, under pulling force capillaceous, test sample and amplification liquid distribute equably rapidly and enter the amplification pond;
2) constant-temperature amplification: with uncured polydimethylsiloxane with the chip involution, isothermal reaction 30 min-60 min in water-bath;
3) result judges: directly whether produces according to white precipitate in the amplification pond and judge detected result, perhaps whether basis produces fluorescence and comes result of determination under ultraviolet lamp.
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| CN102618670A (en) * | 2012-04-18 | 2012-08-01 | 中国检验检疫科学研究院 | Loop-mediated isothermal amplification (LAMP) miniaturized total analysis method for H5 subtype avian influenza viruses |
| CN102634607A (en) * | 2012-04-18 | 2012-08-15 | 中国检验检疫科学研究院 | H9 subtype avian influenza virus loop-mediated isothermal amplification micro total analysis method |
| CN103114033A (en) * | 2012-11-08 | 2013-05-22 | 杭州腾越生物科技有限公司 | Array type multiplex microfluidic isothermal amplification chip for HPV (human papillomavirus) detection |
| CN103667011A (en) * | 2013-10-23 | 2014-03-26 | 国家纳米科学中心 | Micro-fluidic chip for loop-mediated isothermal amplification, preparation method and application of micro-fluidic chip |
| CN104862216A (en) * | 2014-02-21 | 2015-08-26 | 宁波大学 | High-throughput visual totally-enclosed split-type LAMP-LFD detection chip device |
| CN104862206A (en) * | 2014-02-21 | 2015-08-26 | 宁波大学 | Apparatus system for multiple multi-target nucleic acid whole-process closed sample injection |
| CN104877900A (en) * | 2015-05-26 | 2015-09-02 | 大连理工大学 | High-flux quick-detection microfluidic chip directed towards pathogenic microorganism and preparation method for microfluidic chip |
| CN105483293A (en) * | 2016-01-29 | 2016-04-13 | 中国人民解放军疾病预防控制所 | Fulminating-infectious-disease pathogen detecting primer pair and kit |
| CN105482989A (en) * | 2016-01-19 | 2016-04-13 | 广州迪澳生物科技有限公司 | Multi-index detecting micro-fluidic chip and detection method |
| CN106238112A (en) * | 2016-08-27 | 2016-12-21 | 宋波 | A kind of micro-fluidic chip and the application in the qualification and drug sensitive experiment of pathogen thereof |
| CN107058063A (en) * | 2017-06-12 | 2017-08-18 | 博奥生物集团有限公司 | A kind of method for multiple nucleic acid amplified production fluoroscopic examination based on micro-fluidic chip |
| CN107475364A (en) * | 2017-06-30 | 2017-12-15 | 北京百康芯生物科技有限公司 | Drug resistant gene micro-fluidic chip quick detection kit and detection method |
| CN107988046A (en) * | 2018-01-23 | 2018-05-04 | 吉林大学 | Self-absorption multichannel detection of pathogens micro-fluidic chip based on LAMP |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5340747A (en) * | 1992-03-09 | 1994-08-23 | Difco Laboratories, Inc. | Diagnostic microbiological testing apparatus and method |
| CN101351542A (en) * | 2005-07-15 | 2009-01-21 | 阿普尔拉公司 | Fluid processing device and method |
| CN201280550Y (en) * | 2008-05-15 | 2009-07-29 | 港龙生物技术（深圳）有限公司 | Micro-pore panel apparatus for high flux gene detection and parting |
| WO2010024485A1 (en) * | 2008-08-29 | 2010-03-04 | Korea Advanced Institute Of Science And Technology | Apparatus and method for multiple immunoassays on a chip |
| CN101880627A (en) * | 2010-01-28 | 2010-11-10 | 湖南大学 | Biosensor for simultaneously detecting two lignin degrading enzyme functional genes, preparation and detection method and electrochemical detection system thereof |
| CN101914435A (en) * | 2010-05-24 | 2010-12-15 | 博奥生物有限公司 | Microtube device and using method thereof |
-
2011
- 2011-03-30 CN CN2011100784509A patent/CN102199531A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5340747A (en) * | 1992-03-09 | 1994-08-23 | Difco Laboratories, Inc. | Diagnostic microbiological testing apparatus and method |
| CN101351542A (en) * | 2005-07-15 | 2009-01-21 | 阿普尔拉公司 | Fluid processing device and method |
| CN201280550Y (en) * | 2008-05-15 | 2009-07-29 | 港龙生物技术（深圳）有限公司 | Micro-pore panel apparatus for high flux gene detection and parting |
| WO2010024485A1 (en) * | 2008-08-29 | 2010-03-04 | Korea Advanced Institute Of Science And Technology | Apparatus and method for multiple immunoassays on a chip |
| CN101880627A (en) * | 2010-01-28 | 2010-11-10 | 湖南大学 | Biosensor for simultaneously detecting two lignin degrading enzyme functional genes, preparation and detection method and electrochemical detection system thereof |
| CN101914435A (en) * | 2010-05-24 | 2010-12-15 | 博奥生物有限公司 | Microtube device and using method thereof |
Cited By (24)
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| CN102618670A (en) * | 2012-04-18 | 2012-08-01 | 中国检验检疫科学研究院 | Loop-mediated isothermal amplification (LAMP) miniaturized total analysis method for H5 subtype avian influenza viruses |
| CN102634607A (en) * | 2012-04-18 | 2012-08-15 | 中国检验检疫科学研究院 | H9 subtype avian influenza virus loop-mediated isothermal amplification micro total analysis method |
| CN103114033A (en) * | 2012-11-08 | 2013-05-22 | 杭州腾越生物科技有限公司 | Array type multiplex microfluidic isothermal amplification chip for HPV (human papillomavirus) detection |
| CN103667011A (en) * | 2013-10-23 | 2014-03-26 | 国家纳米科学中心 | Micro-fluidic chip for loop-mediated isothermal amplification, preparation method and application of micro-fluidic chip |
| CN104862216A (en) * | 2014-02-21 | 2015-08-26 | 宁波大学 | High-throughput visual totally-enclosed split-type LAMP-LFD detection chip device |
| CN104862206A (en) * | 2014-02-21 | 2015-08-26 | 宁波大学 | Apparatus system for multiple multi-target nucleic acid whole-process closed sample injection |
| CN104877900A (en) * | 2015-05-26 | 2015-09-02 | 大连理工大学 | High-flux quick-detection microfluidic chip directed towards pathogenic microorganism and preparation method for microfluidic chip |
| CN104877900B (en) * | 2015-05-26 | 2017-04-12 | 大连理工大学 | High-flux quick-detection microfluidic chip directed towards pathogenic microorganism and preparation method for microfluidic chip |
| CN105482989A (en) * | 2016-01-19 | 2016-04-13 | 广州迪澳生物科技有限公司 | Multi-index detecting micro-fluidic chip and detection method |
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| CN105483293A (en) * | 2016-01-29 | 2016-04-13 | 中国人民解放军疾病预防控制所 | Fulminating-infectious-disease pathogen detecting primer pair and kit |
| CN109414664A (en) * | 2016-07-06 | 2019-03-01 | 精密纳米系统有限公司 | Intelligent Microfluidic Mixing instrument and kit |
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| CN107988046A (en) * | 2018-01-23 | 2018-05-04 | 吉林大学 | Self-absorption multichannel detection of pathogens micro-fluidic chip based on LAMP |
| CN110452964A (en) * | 2018-05-08 | 2019-11-15 | 国家纳米科学中心 | The method and application of seven pipeline chips detection pathogenic entero becteria salmonella |
| CN109797204A (en) * | 2019-02-22 | 2019-05-24 | 上海交通大学苏北研究院 | A kind of multiple nucleic acid detection method based on discoid capillary microarray |
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| CN114381550A (en) * | 2021-12-03 | 2022-04-22 | 中国科学院精密测量科学与技术创新研究院 | Multi-target nucleic acid detection kit and detection method for HPV typing |
| WO2023098157A1 (en) * | 2021-12-03 | 2023-06-08 | 中国科学院精密测量科学与技术创新研究院 | Multi-target nucleic acid detection kit and detection method for hpv typing |
| CN115161019A (en) * | 2022-05-11 | 2022-10-11 | 华中农业大学 | Preparation method of nitrogen-doped luminescent carbon quantum dot and application of nitrogen-doped luminescent carbon quantum dot in rapid detection of lysine content in pig serum |
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Application publication date: 20110928 |