CN102175739A - Enzyme injection type glucose biosensor - Google Patents
Enzyme injection type glucose biosensor Download PDFInfo
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- CN102175739A CN102175739A CN2010106162247A CN201010616224A CN102175739A CN 102175739 A CN102175739 A CN 102175739A CN 2010106162247 A CN2010106162247 A CN 2010106162247A CN 201010616224 A CN201010616224 A CN 201010616224A CN 102175739 A CN102175739 A CN 102175739A
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Abstract
The invention provides an enzyme injection type glucose biosensor oriented to a fermentation process. The biosensor comprises a working electrode 1, a counter electrode 2, a reference electrode 3, a buffer solution and enzyme solution inlet 4, a buffer solution and enzyme solution outlet 5, a to-be-detected solution inlet 6, a semipermeable membrane 7, a reaction tank 8, a to-be-detected solution tank 9, a magnetic stirring rotor 10, a seal ring 11, a connection hole 12, an electrode fixing body 13, an outer structure body 14 and an enzyme solution quantitative injecting device 15. In the invention, by utilizing an enzyme solution quantitative injection method, high-pressure steam sterilization can be simultaneously carried out on the biosensor along with a fermentation tank so that the reliability of the biosensor is enhanced and the detection accuracy is improved; by combining with FIA (Flow Injection Analysis), the biosensor easily realizes on-line detection of glucose; the enzyme exists in a fresh enzyme solution way, is independent of the solution to be detected, is not influenced by the outside world and needs no frequent calibration; through replacing different enzyme solutions, different materials can be detected with low detection cost; and the biosensor provided by the invention is compact in structure, convenient for processing and easy for mass production.
Description
Technical field
The present invention relates to the glucose detection technology of biology sensor formula, it is the biology sensor that is used to detect glucose of a kind of comprehensive employing physics, chemistry, biology, electronics, specifically, be a kind of enzyme injecting type glucose biological sensor that is applicable to online detection, belong to field of biosensors towards sweat.
Background technology
1962, Clark and Lyons proposed the notion of " enzyme electrode Enzyme electrode " for the first time, and this incident with milestone significance is considered to the initial of biology sensor development, and therefore Clark also obtains the title of " father of biology sensor ".Updike in 1967 and Hicks have successfully made first glucose enzyme electrode biology sensor according to this principle.
The principle of glucose enzyme electrode biology sensor is an electrochemical reaction process, with the glucose oxidase is that example is that electrochemical reaction takes place in reaction tank for glucose oxidase and glucose, the electronics that generates, form electric current, obtain current signal or voltage signal by testing circuit by electrode, thereby realize the detection of glucose.According to the difference of used electron transfer mediator, the glucose enzyme electrode biology sensor can be divided into the three generations.First generation biology sensor with oxygen molecule or hydrogen peroxide as electron transfer medium between enzyme and electrode, the proportional relation of the size of current of generation and oxygen concentration.The second generation adopts artificial electron transfer mediator such as the potassium ferricyanide/potassium ferrocyanide, ferrocene and derivant thereof, dye molecule, organic conductive salt, metal phthalocyanine etc. as the electron transfer medium, and the artificial redox reaction of electron transfer medium on electrode is reversible.The third generation does not need electron transfer mediator, and electron transport directly takes place between electrode and enzyme.
In the sweat, in order to prevent microbiological contamination, online glucose biological sensor and and reactor between pipeline must be able to bear the high-temperature steam sterilization, descend or forfeiture to avoid its performance of sterilization back, so glucose biological sensor mainly is an off-line type at present, online is also at the experimental stage.Yet glucose is reactant and intermediate product common in the fermentation process, and the control to concentration of glucose in the sweat can be directly connected to product productive rate and quality.Thereby the detection of the real-time online of concentration of glucose is very important and necessary.Therefore, extremely need now a kind of be used for being applicable to of sweat online, fast, glucose biological sensor reliable, easy to use.
Summary of the invention
The invention provides a kind of enzyme injecting type glucose biological sensor that is applicable to online detection towards sweat.
Enzyme injecting type glucose biological sensor, it is characterized in that: this sensor comprises: working electrode 1, to electrode 2, contrast electrode 3, damping fluid and enzyme liquid inlet 4, damping fluid and enzyme liquid outlet 5, test solution inlet 6, semi-permeable diaphragm 7, reaction tank 8, tested liquid pool 9, magnetic stirring rotator 10, O-ring seal 11, connecting hole 12, electrode fixed body 13, external structure body 14, enzyme liquid calibrated shot device 15, and concrete annexation is:
Described working electrode 1, electrode 2 and contrast electrode 3 are fixed in the electrode fixed body 13; Electrode fixed body 13 is fixed in the external structure body 14, external structure body 14 is divided into two parts up and down, described connecting hole 12 fixedly connected these two parts, the external structure body is fixed with semi-permeable diaphragm 7 between two parts about in the of 14, by O-ring seal 11 and extraneous sealing, the first half of external structure body 14 and semi-permeable diaphragm 7, working electrode 1, to electrode 2, contrast electrode 3 formation reaction tanks 8; Constitute tested liquid pool 9 between the latter half of external structure body 14 and the semi-permeable diaphragm 7; Be built-in with magnetic stirring rotator 10 in the tested liquid pool 9.
The first half of external structure body 14 has the damping fluid of reaction tank 8 and enzyme liquid inlet 4 and damping fluid and enzyme liquid outlet 5, and described damping fluid and enzyme liquid inlet 4 are connected with the damping fluid bottle with enzyme liquid calibrated shot device; Described damping fluid and enzyme liquid outlet 5 is connected with waste liquid bottle; The first half of external structure body 14 has the test solution inlet 6 of tested liquid pool 9, and test solution inlet 6 is connected with tested liquid pool 9 by the FIA of flow injection system.
Enzyme liquid calibrated shot device 15 is made of electromagnetism six-way valve 16 and quantitative ring 17, quantitatively encircles 17 two ends and is connected in electromagnetism six-way valve 16 any diagonal angle mouths.Electromagnetism six-way valve 16 a bite wherein is connected to damping fluid and enzyme liquid inlet 4.
Electrode 2 is shaped as cylindrical, annular, and material is a conductive material.
Electrode fixed body 13 is shaped as cylindrical, and material is nonmetallic materials.
Described enzyme injecting type glucose biological sensor adopts enzyme liquid calibrated shot mode.
Advantage of the present invention is:
Enzyme exists in the mode of enzyme liquid, promptly is dissolved in damping fluid by enzyme and adjuvant and makes, and is simple to operate;
Enzyme exists in the enzyme liquid mode of " fresh ", and is independent with test solution, is not subjected to ectocine, makes sensor not need frequent calibration;
Change different types of enzyme liquid, can detect different material, promptly a sensor can detect multiple material;
The detection cost is low, about 0.01 yuan of the used enzyme liquid of single cost;
Conveniently combine, realize the online detection of glucose easily with FIA Flow Injection Analysis system;
Adopt enzyme liquid injection system, can realize that sensor carries out online high pressure steam sterilization simultaneously with fermentation tank, not only strengthened the reliability of sensor, and improved the detection degree of accuracy;
The built-in isolated electromagnetic agitation rotor of tested liquid pool makes that the reactant electrochemical reaction is more abundant, and then has improved detection sensitivity;
Invention compact conformation, easy to process is easy to mass production.
Description of drawings
Fig. 1 is the agent structure synoptic diagram of enzyme injecting type glucose biological sensor.
Fig. 2 be with this enzyme injecting type glucose biological sensor in the structural representation of enzyme liquid calibrated shot device.
Among the figure: 1, working electrode, 2, to electrode, 3, contrast electrode, 4, damping fluid and enzyme liquid inlet, 5, the outlet of damping fluid and enzyme liquid, 6, the test solution inlet, 7, semi-permeable diaphragm, 8, reaction tank, 9, tested liquid pool, 10, magnetic stirring rotator, 11, O-ring seal, 12, connecting hole, 13, electrode fixed body, 14, the external structure body, 15, enzyme liquid calibrated shot device, 16, electromagnetism six-way valve, 17, quantitative ring.
Embodiment
As depicted in figs. 1 and 2, structure of the present invention is by working electrode 1, to electrode 2, contrast electrode 3, damping fluid and enzyme liquid inlet 4, damping fluid and enzyme liquid outlet 5, test solution inlet 6, semi-permeable diaphragm 7, reaction tank 8, tested liquid pool 9, magnetic stirring rotator 10, O-ring seal 11, connecting hole 12, electrode fixed body 13, external structure body 14.
Enzyme injecting type glucose biological sensor, it is characterized in that: this sensor comprises: working electrode 1, to electrode 2, contrast electrode 3, damping fluid and enzyme liquid inlet 4, damping fluid and enzyme liquid outlet 5, test solution inlet 6, semi-permeable diaphragm 7, reaction tank 8, tested liquid pool 9, magnetic stirring rotator 10, O-ring seal 11, connecting hole 12, electrode fixed body 13, external structure body 14, enzyme liquid calibrated shot device 15, and concrete annexation is:
Described working electrode 1, electrode 2 and contrast electrode 3 are fixed in the electrode fixed body 13; Electrode fixed body 13 is fixed in the external structure body 14, external structure body 14 is divided into two parts up and down, described connecting hole 12 fixedly connected these two parts, the external structure body is fixed with semi-permeable diaphragm 7 between two parts about in the of 14, by O-ring seal 11 and extraneous sealing, the first half of external structure body 14 and semi-permeable diaphragm 7, working electrode 1, to electrode 2, contrast electrode 3 formation reaction tanks 8; Constitute tested liquid pool 9 between the latter half of external structure body 14 and the semi-permeable diaphragm 7; Be built-in with magnetic stirring rotator 10 in the tested liquid pool 9;
The first half of external structure body 14 has the damping fluid of reaction tank 8 and enzyme liquid inlet 4 and damping fluid and enzyme liquid outlet 5, and described damping fluid and enzyme liquid inlet 4 are connected with the damping fluid bottle with enzyme liquid calibrated shot device; Described damping fluid and enzyme liquid outlet 5 is connected with waste liquid bottle; The first half of external structure body 14 has the test solution inlet 6 of tested liquid pool 9, and test solution inlet 6 is connected with tested liquid pool 9 by the FIA of flow injection system.
Enzyme liquid calibrated shot device 15 is made of electromagnetism six-way valve 16 and quantitative ring 17, quantitatively encircles 17 two ends and is connected in electromagnetism six-way valve 16 any diagonal angle mouths.Electromagnetism six-way valve 16 a bite wherein is connected to damping fluid and enzyme liquid inlet 4.
Wherein, working electrode 1, electrode 2 and contrast electrode 3 are fixed in the electrode fixed body 13, have constituted the electrochemical measurement three-electrode system jointly.
Electrode 2 is shaped as cylindrical, but is not limited to cylindrically, can be such as but not limited to by metal material or conductive material: platinum, gold, silver, graphite etc. are made.
Electrode fixed body 13 is shaped as cylindrical, but is not limited to cylindrically, and can be such as but not limited to by nonmetallic materials: organic glass, pottery, teflon, polyetheretherketone etc. are made.
Before the sensor measurement sample, with buffer solution for cleaning reaction tank 8 and tested liquid pool 9; Then, liquid to be measured is introduced into tested liquid pool 9 by the FIA of Flow Injection Analysis system, starts the magnetic stirring rotator; Then, by enzyme liquid calibrated shot device glucose oxidase liquid is expelled in the reaction tank 8, glucose oxidase liquid and glucose react under the three-electrode system effect in reaction tank 8, produce current signal, draw by working electrode 1, this current signal is by the I/V translation circuit in the three electrode detection circuit, become voltage signal, then to voltage signal amplification, filtering, obtain one and the proportional voltage signal of concentration of glucose to be measured, this voltage signal amplitude is promptly represented liquid concentration of glucose to be measured.
Claims (10)
1. enzyme injecting type glucose biological sensor, it is characterized in that: this sensor comprises: working electrode (1), to electrode (2), contrast electrode (3), damping fluid and enzyme liquid inlet (4), damping fluid and enzyme liquid outlet (5), test solution inlet (6), semi-permeable diaphragm (7), reaction tank (8), tested liquid pool (9), magnetic stirring rotator (10), O-ring seal (11), connecting hole (12), electrode fixed body (13), external structure body (14), enzyme liquid calibrated shot device, and concrete annexation is:
Described working electrode (1), electrode (2) and contrast electrode (3) are fixed in the electrode fixed body (13); Electrode fixed body (13) is fixed in the external structure body (14), external structure body (14) is divided into two parts up and down, fixedly connected these two parts of described connecting hole (12), external structure body (14) is fixed with semi-permeable diaphragm (7) up and down between two parts, by O-ring seal (11) and extraneous sealing, the first half of external structure body (14) and semi-permeable diaphragm (7), working electrode (1), to electrode (2), contrast electrode (3) formation reaction tank (8); Constitute tested liquid pool (9) between the latter half of external structure body (14) and the semi-permeable diaphragm (7); Be built-in with magnetic stirring rotator (10) in the tested liquid pool (9);
The first half of external structure body (14) has damping fluid and the enzyme liquid inlet (4) and damping fluid and enzyme liquid outlet (5) of reaction tank (8), and described damping fluid is connected with the damping fluid bottle with enzyme liquid calibrated shot device with enzyme liquid inlet (4); Described damping fluid is connected with waste liquid bottle with enzyme liquid outlet (5); The latter half of external structure body (14) has the test solution inlet (6) of tested liquid pool (9), and test solution inlet (6) is connected with tested liquid pool (9) by the FIA of flow injection system.
2. according to the described enzyme injecting type of claim 1 glucose biological sensor, it is characterized in that: working electrode (1) is shaped as cylindrical, annular, and material is a conductive material.
3. according to the described enzyme injecting type of claim 1 glucose biological sensor, it is characterized in that: electrode (2) is shaped as cylindrical, long column shape, and material is a conductive material.
4. according to the described enzyme injecting type of claim 1 glucose biological sensor, it is characterized in that: contrast electrode (3) is shaped as cylindrical, annular, and material is Ag/AgCl.
5. according to the described enzyme injecting type of claim 1 glucose biological sensor, it is characterized in that: electrode fixed body (13) is shaped as cylindrical, and material is nonmetallic materials.
6. according to the described enzyme injecting type of claim 1 glucose biological sensor, it is characterized in that: external structure body (14) is made by nonmetallic materials.
7. according to the described enzyme injecting type of claim 1 glucose biological sensor, it is characterized in that: semi-permeable diaphragm (7) is isolated reaction tank (8) and tested liquid pool (9), allow glucose and micromolecule in the test solution to enter reaction tank (8) participation reaction by it, stop that the molecule bigger than glucose molecule passes through, it can be made by polycarbonate membrane or polysulfone membrane etc.
8. according to the described enzyme injecting type of claim 1 glucose biological sensor, it is characterized in that: adopt enzyme liquid calibrated shot mode, enzyme liquid is dissolved in damping fluid by enzyme and adjuvant and makes.
9. according to the described enzyme injecting type of claim 1 glucose biological sensor, it is characterized in that: the built-in magnetic stirring rotator of tested liquid pool (9) (10), it can be made by the nonmetallic materials of band magnetic core.
10. according to the described enzyme injecting type of claim 1 glucose biological sensor, it is characterized in that: change different types of enzyme liquid, can detect different material, promptly a sensor can detect multiple material.
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| CN2010106162247A CN102175739A (en) | 2010-12-31 | 2010-12-31 | Enzyme injection type glucose biosensor |
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| CN2010106162247A CN102175739A (en) | 2010-12-31 | 2010-12-31 | Enzyme injection type glucose biosensor |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102608342A (en) * | 2012-02-29 | 2012-07-25 | 北京工业大学 | Sampling device for enzyme injection type glucose biosensor online analyzer |
| CN103235016A (en) * | 2013-04-23 | 2013-08-07 | 北京工业大学 | Glucose biosensor for online detection |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4025308A (en) * | 1974-06-07 | 1977-05-24 | United Kingdom Atomic Energy Authority | Method and apparatus for monitoring low concentrations of ions in a flowing fluid |
| JPH02208551A (en) * | 1989-02-08 | 1990-08-20 | New Japan Radio Co Ltd | Biosensor |
| US6214206B1 (en) * | 1995-01-03 | 2001-04-10 | Chemel Ab | Use of a chemical sensor |
| CN1981191A (en) * | 2004-07-08 | 2007-06-13 | 凯麦尔股份公司 | SIRE flow detector |
| US7780917B2 (en) * | 2004-08-25 | 2010-08-24 | Chemel Ab | Calibratable flow detector |
-
2010
- 2010-12-31 CN CN2010106162247A patent/CN102175739A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4025308A (en) * | 1974-06-07 | 1977-05-24 | United Kingdom Atomic Energy Authority | Method and apparatus for monitoring low concentrations of ions in a flowing fluid |
| JPH02208551A (en) * | 1989-02-08 | 1990-08-20 | New Japan Radio Co Ltd | Biosensor |
| US6214206B1 (en) * | 1995-01-03 | 2001-04-10 | Chemel Ab | Use of a chemical sensor |
| CN1981191A (en) * | 2004-07-08 | 2007-06-13 | 凯麦尔股份公司 | SIRE flow detector |
| US7780917B2 (en) * | 2004-08-25 | 2010-08-24 | Chemel Ab | Calibratable flow detector |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102608342A (en) * | 2012-02-29 | 2012-07-25 | 北京工业大学 | Sampling device for enzyme injection type glucose biosensor online analyzer |
| CN103235016A (en) * | 2013-04-23 | 2013-08-07 | 北京工业大学 | Glucose biosensor for online detection |
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Application publication date: 20110907 |