CN102133391A - Autoserum compound for nourishing skin mesoderm and preparation method thereof - Google Patents
Autoserum compound for nourishing skin mesoderm and preparation method thereof Download PDFInfo
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- CN102133391A CN102133391A CN2011100464093A CN201110046409A CN102133391A CN 102133391 A CN102133391 A CN 102133391A CN 2011100464093 A CN2011100464093 A CN 2011100464093A CN 201110046409 A CN201110046409 A CN 201110046409A CN 102133391 A CN102133391 A CN 102133391A
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Abstract
The invention discloses an autoserum compound for nourishing the skin mesoderm and a preparation method thereof. The autoserum compound for nourishing the skin mesoderm is characterized by comprising the following raw materials in percentage by weight: 65-80% of fresh sterile autoserum, 15-30% of sterile phosphate buffered normal saline injection of which the concentration is 0.1mol/L and the pH is 7.2, 0.05-0.15% of sterile rh-bFGF (recombinant human basic fibroblast growth factor) freeze-dried powder, 0.05-0.20% of medicinal glycerol, 0.1-0.3% of medicinal mannitol and 0.1-0.3% of medicinal glycine. Compared with the commonly used autoserum, under the same effect, the autoserum compound disclosed by the invention has the advantage of less actual dosage, can be better cultured in the body tissue and filled into the skin pit, and has a very ideal effect on eliminating wrinkles. The autoserum compound disclosed by the invention has simple preparation processes, is easy in actual operation, has no need of special instruments, and is convenient to use.
Description
Technical field
The invention belongs to aesthetic health care and skin anti-aging and medical biotechnology field, relate to a kind of autoserum complex that is used for the nutrition of skin mesoderm and preparation method thereof.
Background technology
Autoserous main component and basic biological characteristics
Autoserum be the blood that extracts healthy person or patient through separate out naturally or centrifugalize after obtain.Serum is that exsomatize blood various thrombins and mechanism thereof in blood starts and solidifies the light yellow transparent liquid that disengages with the blood clot bunching.Autoserum can provide complete nutrition ingredients (sugar, fat, protein, aminoacid, vitamin, inorganic salt, trace element, nucleic acid) and bioactive substance (multiple hormone, enzyme, cell growth factor, antibody and glycolipid, glycoprotein, mucin, mucopolysaccharide etc.) for the cell growth, these materials can effectively improve the skin tissue cell vigor, impel the division and the propagation of skin primary cell simultaneously.Especially in recent years, the relation of somatomedin and skin aging more and more comes into one's own.For example: insulin like growth factor has mitogenesis and facilitates the effect of fibrocyte breeding; Vascular endothelial cell growth factor all can promote the division growth of vascular endothelial cell in vivo and in vitro, stimulates new vessels to form; Epithelical cell growth factor can promote the in-vitro multiplication of keratinocyte.These somatomedin not only can promote growth, the propagation of cell etc., and good synergism is also arranged between them.Simultaneously, autoserum has short contact to be provided and to stretch the factor makes cell attachment avoid mechanical damage and the characteristics that conjugated protein participation cellular metabolism is provided, also contain trace element and ion as the prothetic group of some important enzyme, as Zn, Se, Mn, Ge etc., they can play an important role at aspects such as antioxidation, and contain a large amount of enzyme in the blood component, for example SOD (removes O
- 2); CAT (removes active H
2O
2), these materials can be to the damage of free radical resisting.Autoserum is applied among medical cosmetology, the plastic surgery gradually because of it can provide needed abundant nutrition composition for cell growth and skin histology.In addition, its application in preclinical medicine, clinical medicine treatment also more and more widely.
The application of autoserum in the dermatology beauty treatment
With advancing age, add the erosion that suffers ultraviolet, harmful substance for a long time, skin is one of the most tangible organ in the body aging process, with other organs of human body the same it the epidermis attenuation can take place, degeneration, water content reduce, elasticity disappears, atrophy, wrinkling, gloomy tarnish, shallow table tiny blood vessels expansion, old mottle, aged gradually problems such as miopragia or forfeiture.The Histological change of skin aging is mainly the elastic fiber increase and collagen protein reduces, when skin is old and feeble gradually, arrangement between the two is also gradually disorderly, wrinkle appears in skin, aging phenomenons such as mottle are to reduce just because of collagen contents in the corium to cause skin corium elasticity and water conservation degree to reduce, thereby cause thinning of skin, it mainly is because matrix metalloproteinase (Matrix metalloproteinases that molecules changes, MMPs) express increase, epidermal hyperplasia and branch voltinism sign abnormal expression, and the main effect of MMPs is dermal tissues such as degrade collagen and elastin laminin.Simultaneously, the number of blood vessel of old people's skin reduces 30% at least, and along with increasing the weight of of skin aging, hypovascular number is more general.And being reflected in cellular level, the aging of skin presentation is cell ageing, fibroblast derives from mesoderm, be to change one of the most obvious and the most responsive cell, it can synthesize and secrete collagen protein and elastin laminin, and then progressively form collagen fiber, reticular fiber, elastic fiber, the improvement of proof wrinkles of skin such as Martin DE depends on reinventing of dermal collagen.Yet dermal collagen reinvent crucial one of them be to promote growth of fibroblasts.
And autoserum is abundant, various biological characteristics can promote mesoblastema (comprising fibroblast, vascular endothelial cell etc.) division, propagation, promotes regenerative cell and has the quantity of vigor cell to be on the increase, and constantly produces collagen protein, elastin laminin, hyaluronic acid, compositions such as dermatan can promote the regeneration of skin new vessels simultaneously, thereby reinvent skin texture, skin presents elasticity, and light is tender, and is ruddy, wrinkle such as smooths at excellent cosmetic result, and the skin aging symptom is improved.Discoveries such as Crowston, with people's tendon fibroblast that human serum is cultivated, apoptosis quantity reduces, molecular marker for increased proliferation, growth conditions is good, and the cytokine in the human serum can suppress the cytotoxic effect of mitogen-toxin c.
Autoserum has provides characteristics biology such as rich nutrient substances and multiple somatomedin for cell growth, thereby can promote the propagation of cell, the reparation of tissue, and provide best growing environment for it, make it in medical cosmetology, plastic surgery's (skin repair treatment, surgical plastic) shows its special advantages in, and obtained good effect.In addition owing to come it to come from from body, no immunologic rejection, complication is few, safe characteristics.In this simultaneously, autoserum is made simple, and convenient sources is damaged less the patient, can effectively reduce medical treatment cost, has alleviated patient's financial burden to a certain extent.The characteristics that autoserum is shown clinically are for it further provides more wide prospect in the treatment of cosmetic plastic surgery.
At present, many methods are being arranged aspect the dermatology beauty and shaping, pharmacotherapy for example, chemistry strips off, the photon delicate skin art, Botulinum toxin is transplanted from the body living cells, the material of the soft tissue that injection is filled etc.Once occurred the use Cera Flava in history, materials such as vaseline are filled skin, reach the beautifying skin effect, and there are fatal shortcomings such as rejection in these materials; Polyalcohol hydrogel has appearred in the modern times in succession, collagen, acellular dermal matrixes etc. are abiotic, biomaterial, simultaneously the autologous tissue aspect mainly adopts autologous fat, and from the molten slurry of body fibroblast etc., but these two kinds still exist the preparation cost height from the body mode of drawing materials, the infection rate height increases multiple shortcomings such as patient suffering; And the untoward reaction of having found to have occurred clinical of injection hyaluronic acid and derivant thereof, the use of injection creotoxin in beauty treatment poisoning occurred and it can only be kept 4-6 month, repressed nerve conduction function is recovered gradually after 4-6 month, muscle contraction, little wrinkle can occur again, so need injection once more at interval after 4～6 months, its cost height, cost an arm and a leg, it is limited to originate, and promotes the use of limited; The cell of transplanting the beauty treatment injection from the body living cells is alive, and is lived, and the emiocytosis collagen protein needs a physiological process, the produce effects that it can be progressive.Be that after injecting the last time 1～2 month begins to take effect generally speaking, optimum efficiency began to present after five or six months.
By contrast, autoserum is a kind of convenient sources, safety non-toxic, aseptic, and it is reusable, economic autologous tissue does not exist that allosome or xenohistocompatibility are poor, the immunological rejection phenomenon and the autoserum of blood type do not contain antiseptic, are that other reagent does not possess.In this simultaneously,, reduced infection chance, can reduce patient's misery for distinguishing and being distinguished non-incision.Add autoserum because the uniqueness of its biological characteristics, multiformity and safe, good stability etc., autoserum is at beautifying skin, wound repair, aspects such as surgical plastic show its special advantages gradually, simultaneously, it also possesses easy preparation, cheap characteristics.Therefore, it can be used as emerging product and fills up the blank that autologous tissue fills.
Yet, use autoserum in shaping and beauty, the best administration concentration of our still indeterminate autoserum, route of administration, optimal dose, and its metabolic way, whether need repeatedly duplicate injection repeatedly, can not determine the observing time after whether autoserum has good late result and treatment.And, traditional method often adopts direct cytotoxicity that the injection of the pure autoserum of high concentration may cause and growth and propagation to suppress even directly causes cell injury, destruction and can't grow, so, should study fully and pay close attention to that it adds that concentration, administration time, potentiation synergist are composite, different medium etc. is to the influence and the intervention thereof of its effective biological action, and the enlarge-effect of great attention effective mechanism, to improve or to improve autoserum in the academic technology level aspect the beauty treatment.
Summary of the invention
The objective of the invention is provides a kind of autoserum complex that is used for the nutrition of skin mesoderm at above-mentioned existing in prior technology problem.
Another object of the present invention provides this preparation method that is used for the autoserum complex of skin mesoderm nutrition.
1, to achieve these goals, the present invention adopts following technical proposals:
A kind of autoserum complex that is used for the nutrition of skin mesoderm of the present invention is characterized in that it is made up of the following weight proportion raw material:
A kind of autoserum complex that is used for the nutrition of skin mesoderm of the present invention is characterized in that it is made up of following preferred weight proportion raw material:
A kind of autoserum complex that is used for the nutrition of skin mesoderm of the present invention is characterized in that described a kind of preparation method that is used for the autoserum complex of skin mesoderm nutrition may further comprise the steps:
(1) in strict accordance with the standardization requirement of human body blood drawing, gather rapidly the operation receiveing person elbow medium-sized vein blood, and collect simultaneously in the aseptic dry blood taking tube that does not contain anticoagulant; Naturally leave standstill about 1 hour, in room temperature with 4000rpmmin
-1The centrifugal 10min of rotating speed; At this moment, the blood in the visible blood taking tube after centrifugal of naked eyes has been divided into three layers: the upper strata light yellow transparent liquid is a serum; Middle very thin one deck canescence is leukocyte and platelet; Bottom one deck redness is an erythrocyte;
(2) in strict accordance with aseptic requirement and above-mentioned preferred weight proportioning, the limpid transparent faint yellow liquid of the superiors that centrifugalize is good is drawn the autoserum of formula ratio with the special-purpose asepsis injector of coupling, and carefully be transferred in the special special-purpose sealed, sterile serum collecting pipe and (include the medicinal glycerin of formula ratio, medicinal glycine, medicinal mannitol, recombination human basic fibroblast growth factor and pH7.2 sterile phosphate buffer saline injection), gently behind the mix homogeneously, be a kind of autoserum complex that is used for the nutrition of skin mesoderm, place 4 ℃ of refrigerators preservations and standby immediately.
2, advantage of the present invention
The invention has the advantages that with used in the past skin wrinkle removing injection and compare, the autoserum that is used for the nutrition of skin mesoderm of the present invention can rest on the skin mesoderm, reach effect in the body cultured tissue, can fill up depression bigger in the skin, as repair wrinkle, skin depressions is filled up skin, and effect is very good.And autoserum, no rejection and toxic and side effects, therapeutic effect is remarkable, treatment back skin nature, the retention time is permanent.Its characteristics are as follows:
(1) core of present technique, be exactly the various cells of cultivating the skin disappearance at the human body skin mesoderm, impel the collagen protein of problem skin part, elastic fibers carries out reparative regeneration, skin presents elasticity, and light is tender, and is ruddy, wrinkle the youth such as smooths and beautifully reproduces on one's body the patient again, and cosmetic result is that general operation or soft tissue are filled incomparable.And contain antibody in the autoserum, can play bacteriostasis.
(2) the present invention's autoserum complex prescription of being used for the nutrition of skin mesoderm brings into play to greatest extent that mutual aid is collaborative, the effect of synergy, makes it to reach more effectively and fast expection beautifying face skin care and skin anti-aging effect.
(3) the present invention adopts best mesoblastema to activate the serum ratio of nourishing and division growth, and provides mesoblastema to mate most, need most and best natural full nutrient composition, optimal pH condition, ion concentration, osmotic pressure, O
2And CO
2The best microenvironment of proper ratio and cell growth, existence, life, science in fact also can obtain more effectively beautifying face skin care and skin anti-aging effect with rationally more in theory.
(4) the present invention adopts best autoserum and other several additives to carry out compound preparation, not only provide and built the microenvironment condition of best mesoblastema existence, growth and life, help mesoblastemic division and propagation and vital movement more, and, also effectively overcome and solved traditional autoserum injection and adopt direct cytotoxicity that the pure autoserum injection of high concentration may cause and growth and propagation to suppress even directly cause cell injury, destruction and can't grow, this also is one of important innovative point of the present invention.
(5) the present invention carries out necessary dilution according to autoserum and other additive, not only break and improved the limitation and the drawback of the pure autoserum injection of traditional autoserum injection applying high density, science and the effectiveness and the reasonability of treatment have been improved, and, obtaining the treatment liquid of same volume, this therapy needs autoserous actual amount only to use 3/4 of serum amount for traditional method, prove by experiment, this concentration, the mesoblastemic growth of the most suitable skin, therefore, technological progress of the present invention not only reduces or has reduced the treatment cost greatly, and, the minimizing of actual blood drawing amount can alleviate or eliminate experimenter's all scrupulous and bad mental reaction influence greatly.
(6) simple, convenient and simple for operation easy of preparation method of the present invention, be easy to promote, be fit to have above use of grass-roots unit of legal medical and beauty treatment qualification.
3, the mechanism of action of autoserum complex of the present invention
Because the skin of human body face is exposed among the external environment all the time, not only suffers the erosion of various harmful pollutants in the nature atmosphere, also suffers the sunlight middle-ultraviolet lamp to cross the damage that irradiation of a specified duration causes; And, also have a lot of people, particularly women also to exist because of using the destruction of the skin of face epidermal barrier 26S Proteasome Structure and Function due to some stripping off property cosmetics etc., add that human body is along with the variation that increases age, naturally-aged that skin showed or physiological aging etc., therefore, science, to carry out the beautifying face skin care be very important and necessary rationally, in good time.
The present invention mainly is prepared into a kind of autoserum complex that is used for the nutrition of skin mesoderm by implementing the dilution autoserum, its curative effect mechanism of action may be relevant with following factor: skin histology and cell need suitable environment and essential condition just can better survive, growth and life (vital movement), and blood constitutent (erythrocyte, leukocyte, platelet, serum and inaemia condensate etc.) in contain cell the most basic needed nutrient substance and cell growth factor etc., particularly the needed various nutritional labelings of skin histology that is contained in the autoserum and cell mainly comprise body fluid, carbohydrate, lipid, protein, peptide, aminoacid, vitamin, inorganic salt, trace element, hormone, enzyme, nucleic acid, antioxidant, cell growth factor and other composition and energy etc.Aminoacid is the ultimate unit of constitutive protein matter, it also is the most basic nutrient of cell, vitamin is a bioactive substance of keeping the cell growth, carbohydrate can provide the energy of cell, inorganic salt be cell form must and participate in the metabolism of cell and the regulation and control of cell micro-environment intermediate ion concentration, the promoting growth of cell factor can promote and accelerate cell growth promoter and division growth; In addition, autoserum also possesses physiology and biochemistry limit interior best ion concentration, gas phase content (oxygen and carbon dioxide content), osmotic pressure and the PH condition etc. of cells survival and propagation, it is the optimal environmental condition of existence of ectoderm and mesoblastema and growth, also be the good culture medium that regenerative cell divides and breeds in the skin histology, make autoserum extremely help the metabolism of cell in the vital movement process.
The autoserum complex that has added synergist (medicinal glycerin, medicinal glycine, medicinal mannitol, recombination human basic fibroblast growth factor and pH7.2 sterile phosphate buffer saline injection) is more conducive to mesoblastema (comprising fibroblast, vascular endothelial cell etc.) division and propagation, and regenerative cell or great-hearted cell quantity are on the increase; Simultaneously, increase by this cell division propagation and young cell quantity, constantly promote compositions such as synthetic new skin histology and the needed structure organization of intercellular substance and bioactive ingredients and collagen protein, elastin laminin, acid mucopolysaccharide, glycoprotein, hyaluronic acid, dermatan, and promote the regeneration of skin new vessels, make the skin corium structure of factor affecting such as suffering physiological, pathologic, environmental condition originally and damage and component thereof be repaired or regenerate, thereby, in the skin histology mesoderm, realize beautifying face skin care and skin anti-aging effect and effect.
4, the present invention prepares points for attention
(1) the autoserous blood sampling site of the present invention derives from elbow medium-sized vein or other ulnar veins usually, not obvious or gather inconvenience, add when the blood consumption is not too big as ulnar vein, also can gather hand back vein and external malleolus vein etc., but the preferably empty stomach venous blood of gathering, to reduce lipidemia etc.
(2) gather blood before, should conscientiously check in the blood collection tube whether dry and sterilization, also answer the scrutiny blood taking needle whether to hold water and be connected with blood collection unit; In addition, whether blood collection tube puts (be strictly on guard against and smash) well.In blood collection procedure, however especially should be with blood along the tube wall of blood collection tube from slowly flowing into, forbid violent jolting, be strictly on guard against that blood produces bubble, in order to avoid haemolysis occurs and cause the plasma fibrinogen degeneration.
(3) in the blood collection procedure, " have a fainting spell during acupuncture treatment " or blood drawing to occur not flurried when dizzy, can allow its flat bed rest can recover for a moment immediately as indivedual operation receiveing persons.In case of necessity, can allow it smell and inhale fragrant ammonia spit of fland.As recovery yet, but acupuncture points such as then acupuncture (or thumb is pinched) philtrum and connected valleys, until reviving.
(4) hypoglycemic reaction and tonicity shock to take place in order preventing to draw blood, should at first to carry out operation receiveing person's ideological work, eliminates its nervous psychology.After blood drawing is finished, also can advise the spoon meat of some beverages of operation receiveing person's adequate diet, gruel and so on; Especially should note understanding and observation operation receiveing person general reaction situation, tightly prevent accidents such as hypertension and cardiovascular diseases.
(5) should be strictly on guard against the haemolysis that hematoclasis occurs.Usually haemolysis mainly is because syringe and container are moist, unclean; Perhaps puncture not smoothly, blood stasis time of pressing vascular or tourniquet to cause during blood drawing oversize etc. causing.For fear of this phenomenon, the tourniquet pressing time does not generally surpass 5 minutes, is preferably in 0.5～1.0 minute and finishes puncture and blood collection smoothly.If venous blood collection puncture not smoothly, tissue injury too much or blood sampling speed is too fast, blood produces a large amount of bubbles when entering the blood collecting tubular container or with excessively concussion or excessive etc. all can cause hematoclasis effectively, cause haemolysis of blood collection tube.If haemolysis is arranged, this serum had better not use.
(6) for running into the patient who suffers from hyperlipemia, in order to prevent lipidemia or chylemia, light diet, the collection simultaneously venous blood more than 12 hours is on an empty stomach adhered in the several leading sky of recommended therapy.Generally, situations such as lipidemia or chylemia, haemolysis, jaundice blood are not suitable for the present invention.
(7) it should be noted especially, when blood drawing, forbid the bubble or the air of hemostix are strayed into to vein, and, syringe can only outwards be taken out, definitely can not push away to intravenous when blood drawing, in order to avoid the injection air is entered, forms aeroembolism and causes serious consequence even life danger.
(8) it is damaged to prevent especially that blood collection tube from occurring in centrifugal process.For fear of this kind situation occurring, the centrifuge tube that one side is collected blood can adopt the plastic that meets the injection requirement, and on the other hand, the centrifugal preceding symmetrical balance of centrifuge jar weight that must keep is with equal.
(9) to note keeping the blood of being gathered, and in time centrifugal with separate.Serum after the centrifugalize should be sub-packed in the autoserum collecting pipe immediately.Autoserum is preferably now adopted existing usefulness, the same day or is once used up, to keep the fresh and therapeutic effect of blood.Strictly according to the facts at exhaustless serum, salable being stored among the cryogenic refrigerator, but the time surpass 3 days, and when reusing or treat, should place 37 ℃ of waters bath with thermostatic control rewarming and reinforcement a little.
The specific embodiment
Further specify product of the present invention and preparation method below in conjunction with specific embodiment
Embodiment 1: the preparation method of synergist in the autoserum complex
The synergist that is used for preparing the autoserum complex is made up of following preferred weight proportion raw material:
Be used for preparing the concrete preparation method (100g is an example with preparation) of the synergist of autoserum complex:
(1) in the preparation container of sterilizing, adds 0.1mol/L sterile phosphate buffer saline (pH7.2) the injection 78.64g of formula ratio 80%; Medicinal glycerin 0.5g, medicinal glycine 0.5g, medicinal mannitol 0.5g are dissolved in wherein, and placed 15 minutes the back that stirs, and in the container of packing into, sealing is in 100 ℃ of sterilization 30min;
(2) get above the sterilization after liquid, add aseptic recombination human basic fibroblast growth factor lyophilized powder 0.2g, add the remaining 0.1mol/L sterile phosphate of formula ratio buffer saline (pH7.2) injection 19.66g again, use ultrafiltration membrance filter, be the synergist that is used for preparing the autoserum complex, divide then to install to (every pipe 3ml) in the special-purpose sealed, sterile serum collecting pipe.
Embodiment 2: a kind of preparation method that is used for the autoserum complex of skin mesoderm nutrition
A kind of autoserum complex that is used for the nutrition of skin mesoderm is made up of following preferred weight proportion raw material:
A kind of concrete preparation method (10ml is an example with preparation) that is used for the autoserum complex of skin mesoderm nutrition:
(1) in strict accordance with the standardization requirement of human body blood drawing, gather rapidly the operation receiveing person about 20 milliliters of elbow medium-sized vein blood, and collect simultaneously in the aseptic dry blood taking tube that does not contain anticoagulant.
(2) 20 milliliters of blood will having collected are after room temperature leaves standstill about 1 hour, and it is centrifugal that two blood collection tube symmetry are put into centrifuge, and adjusting needs centrifugal rotation speed and time (4000rpmmin
-1, centrifugal 10 minutes) carry out centrifugal.Unit with good conditionsi preferably adopts refrigerated centrifuge, and centrifugal indoor temperature preferably is controlled between 5-15 ℃ and gets final product.
(3) close centrifuge immediately behind centrifugal the finishing, carefully take out blood collection tube (centrifuge tube), at this moment, the blood in the visible centrifuge tube of naked eyes has been divided into three layers: the upper strata light yellow transparent liquid is a serum; Middle very thin one deck canescence is leukocyte and platelet; Bottom one deck redness is an erythrocyte.Simultaneously, notice that the outward appearance of observing supernatant has or not haemolysis, jaundice blood and lipidemia.
(4) the limpid transparent faint yellow liquid-autoserum of the superiors that centrifugalize is good, adopt autoserum to shift special-purpose syringe and draw 7 milliliters respectively, carefully be transferred in the special special-purpose sealed, sterile serum collecting pipe (including 3 milliliters of the medicinal glycerin, medicinal glycine, medicinal mannitol, recombination human basic fibroblast growth factor of formula ratio and pH7.2 sterile phosphate buffer saline injection), gently behind the mix homogeneously, be a kind of autoserum complex that is used for the nutrition of skin mesoderm, place 4 ℃ of refrigerators preservations and standby immediately.
Claims (3)
1. autoserum complex that is used for the nutrition of skin mesoderm is characterized in that it is made up of the following weight proportion raw material:
2. a kind of autoserum complex that is used for the nutrition of skin mesoderm according to claim 1 is characterized in that it is made up of following preferred weight proportion raw material:
3. according to claim 1 or the described a kind of autoserum complex that is used for the nutrition of skin mesoderm of claim 2, it is characterized in that described a kind of preparation method that is used for the autoserum complex of skin mesoderm nutrition may further comprise the steps:
(1) in strict accordance with the standardization requirement of human body blood drawing, gather rapidly the operation receiveing person elbow medium-sized vein blood, and collect simultaneously in the aseptic dry blood taking tube that does not contain anticoagulant; Naturally leave standstill about 1 hour, in room temperature with 4000rpmmin
-1The centrifugal 10min of rotating speed; At this moment, the blood in the visible blood taking tube after centrifugal of naked eyes has been divided into three layers: the upper strata light yellow transparent liquid is a serum; Middle very thin one deck canescence is leukocyte and platelet; Bottom one deck redness is an erythrocyte;
(2) in strict accordance with aseptic requirement and above-mentioned preferred weight proportioning, the limpid transparent faint yellow liquid of the superiors that centrifugalize is good is drawn the autoserum of formula ratio with the special-purpose asepsis injector of coupling, and carefully be transferred in the special special-purpose sealed, sterile serum collecting pipe and (include the medicinal glycerin of formula ratio, medicinal glycine, medicinal mannitol, recombination human basic fibroblast growth factor and pH7.2 sterile phosphate buffer saline injection), gently behind the mix homogeneously, be a kind of autoserum complex that is used for the nutrition of skin mesoderm, place 4 ℃ of refrigerators preservations and standby immediately.
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| CN1090753A (en) * | 1993-02-09 | 1994-08-17 | 朱钧 | Cosmetics containing animal blood serum |
| US20050271633A1 (en) * | 1997-02-20 | 2005-12-08 | Kleinsek Don A | Issue defect augmentation and repair with in vitro cultured fibroblasts |
| CN101057823A (en) * | 2004-01-15 | 2007-10-24 | 方昌阁 | Composite biological preparation with antisenility and beautifying function |
| CN101327172A (en) * | 2008-07-23 | 2008-12-24 | 冯来坤 | Alkaline fibroblast growth factor and use of its composition in preparing beauty treatment cosmetics |
-
2011
- 2011-02-25 CN CN2011100464093A patent/CN102133391A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1090753A (en) * | 1993-02-09 | 1994-08-17 | 朱钧 | Cosmetics containing animal blood serum |
| US20050271633A1 (en) * | 1997-02-20 | 2005-12-08 | Kleinsek Don A | Issue defect augmentation and repair with in vitro cultured fibroblasts |
| CN101057823A (en) * | 2004-01-15 | 2007-10-24 | 方昌阁 | Composite biological preparation with antisenility and beautifying function |
| CN101327172A (en) * | 2008-07-23 | 2008-12-24 | 冯来坤 | Alkaline fibroblast growth factor and use of its composition in preparing beauty treatment cosmetics |
Non-Patent Citations (3)
| Title |
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| 《Ann Dermatol》 20111231 Dae Hun Kim Can Platelet-rich Plasma Be Used for Skin Rejuvenation Evaluation of Effects of Platelet-rich Plasma on Human Dermal Fibroblast , * |
| 《Clinics in Dermatology》 20081231 Matilde Iorizzo Biorejuvenation theory and practice , * |
| 《Dermatologic Surgery》 20061231 Snehal P. Amin et al Mesotherapy for facial skin rejuvenation a clinical, histologic, and electron microscopic evaluation , * |
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Application publication date: 20110727 |