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Publication numberCN101975769 A
Publication typeApplication
Application numberCN 201010284908
Publication date16 Feb 2011
Filing date17 Sep 2010
Priority date17 Sep 2010
Publication number201010284908.1, CN 101975769 A, CN 101975769A, CN 201010284908, CN-A-101975769, CN101975769 A, CN101975769A, CN201010284908, CN201010284908.1
Inventors刘丽娜, 李伟华, 李步洪, 聂英斌, 谢树森, 陈德福, 黄志勇
Applicant福建师范大学
Export CitationBiBTeX, EndNote, RefMan
External Links: SIPO, Espacenet
Human tissue autofluorescence detection system based on excitation of light sources with different wavelength
CN 101975769 A
Abstract
The invention relates to an autofluorescence detection system for exciting specific endogenous fluorescent substances in a human tissue by light sources with different wavelengths and detecting information of fluorescence with specific wavelength corresponding to the fluorescent substances in endoscopy for the human early tumor tissue. The autofluorescence detection system mainly consists of an excitation light source part, an excitation-detection-acquisition part, an optical collection part, a signal preprocessing part and a computer part. The autofluorescence detection system is characterized in that a tunable laser (1) can adjust the needed pulse laser wavelengths according to the specific endogenous fluorescent substances in the human tissue, and a filter wheel (10) is equipped with narrow band pass filters with different center wavelengths. The system can excite the specific endogenous fluorescent substances in the human tissue by a plurality of optimal excitation wavelengths sequentially, thus avoiding the problem of no benefit for analysis owing to insufficient fluorescence information or information overlap and the like caused by excitation of single wavelength or broad spectrum light sources, and improving sensitivity and specificity of early tumor detection.
Claims(4)  translated from Chinese
  1. 一种基于不同波长光源激发的人体组织自体荧光检测系统,主要由激发光源部分、激发‑检测‑采集部分、光学收集部分、信号预处理部分和计算机部分构成,其中信号预处理部分与计算机部分通过信号线进行连接,激发光源部分通过激发光与激发‑检测‑采集部分中的激发光输入端的光纤相连,激发‑检测‑采集部分中的荧光输出端的光纤通过荧光信号通路与光学收集部分相连,荧光信号通路经过光学收集部分传输后进入信号预处理部分,信号预处理部分中光电倍增管光电转化后变为电信号,进入计算机部分,其特征在于可调谐激光器(1)可以根据人体组织中特定的内源性荧光物质调节所需的脉冲激光波长,滤光轮(10)上安装有不同中心波长的窄带滤光片。 Based on human tissue autofluorescence excitation light of different wavelengths detection system, mainly by the excitation light source part, inspired - detection - acquisition part, an optical collection part, signal preprocessing and computer parts, where the signal preprocessing part by computer signal line connection, the excitation light source part by the excitation light and excitation - detection - collection portion of the excitation light input end of the fiber is connected to inspire - detection - acquisition part of the fluorescent output of the fiber by the fluorescent signal path connected to the optical collection section, fluorescence After the optical signal path to collect part of the transmission signal preprocessing section into the signal preprocessing section photomultiplier tube after photoelectric conversion into electrical signals, into the computer part, characterized in that the tunable laser (1) according to specific human tissues endogenous fluorescent substance regulating the desired pulse laser wavelength, the installation has different center wavelength of narrow band filters of the filter wheel (10).
  2. 2.根据权利要求1所述的一种基于不同波长光源激发的人体组织自体荧光检测系统, 其特征在于所述的可调谐激光器可依次调节的波长范围从210nm到2200nm。 2. A according to claim 1, wherein the tissue autofluorescence-based detection systems excitation light sources of different wavelengths, characterized in that the wavelength range of the tunable laser can be successively adjusted from 210nm to 2200nm.
  3. 3.根据权利要求1所述的一种基于不同波长光源激发的人体组织自体荧光检测系统, 其特征在于所述的滤光轮上安装有不同中心波长的窄带滤光片。 3. According to one of claim 1, wherein the tissue autofluorescence-based detection systems excitation light sources of different wavelengths, characterized in that said filter wheel installed on different central wavelength narrowband filter.
  4. 4.根据权利要求1所述的一种基于不同波长光源激发的人体组织自体荧光检测系统, 其特征在于可以通过计算机上的同步控制软件,实现可调谐激光器输出的激发光波长与滤光轮上相对应的窄带滤光片的同步转换。 A according to claim 1, wherein the body tissue based on the excitation light sources of different wavelengths of autofluorescence detection system, characterized by synchronous control software on a computer, implement the tunable laser output from the excitation light wavelength and the filter wheel narrowband filter corresponding synchronous conversion.
Description  translated from Chinese

一种基于不同波长光源激发的人体组织自体荧光检测系统 Based on tissue excitation light sources of different wavelengths of autofluorescence detection system

技术领域 FIELD

[0001] 本发明属生物医学类仪器,涉及一种人体组织自体荧光检测系统,尤其是一种可用于人体早期肿瘤组织的内窥镜检查,利用不同波长光源激发人体组织中特定的内源性荧光物质,并检测与这些荧光物质相对应的特定波长荧光信息的自体荧光检测系统。 [0001] The present invention belongs to the class of biomedical instruments, involving a human tissue autofluorescence detection system, especially one that can be used early endoscopy human tumor tissue, using different wavelength light to stimulate the body tissue specific endogenous a fluorescent substance, and detecting a fluorescent substance corresponding to the autofluorescence of these specific wavelength fluorescence detection system information.

技术背景 Technical Background

[0002] 现有的人体组织自体荧光检测系统是通过利用普通内窥镜,并采用单一波长或宽光谱的光源激发人体组织的内源性荧光物质,利用正常和肿瘤组织的自体荧光光谱差异对人体组织进行荧光检测的系统。 [0002] existing human tissue autofluorescence detection system is through the use of ordinary endoscope, and the use of a single wavelength or a broad spectrum of light to stimulate endogenous fluorescent substances of human tissue, the use of normal and tumor tissue autofluorescence spectroscopy differences human tissue fluorescence detection system. 人体组织的自体荧光主要来源于氨基酸、结构蛋白、 酶、辅酶、脂肪、维生素和卟啉等物质,它们各自具有不同的最佳荧光激发波长和与之对应的荧光发射波长,其中常见的内源性荧光物质如色氨酸、胶原蛋白、还原型烟酰胺腺嘌呤二核苷酸、吓啉和黄素腺嘌呤二核苷酸等所对应的最佳激发波长分别为280、325、340、400 和450nm,相应的最佳荧光发射波长分别为335、390、460、635和535nm。 Autofluorescence of human tissue mainly from amino acids, structural proteins, enzymes, coenzymes, fat, vitamins and porphyrin and other substances, which each have different optimal excitation wavelength and the corresponding fluorescence emission wavelength, wherein the common source fluorescent substances such as tryptophan, collagen, reduced nicotinamide adenine dinucleotide, scared morpholine flavin adenine dinucleotide and the like corresponding to the optimal excitation wavelengths were 280,325,340,400 and 450nm, corresponding optimal fluorescence emission wavelengths were 335,390,460,635 and 535nm. 在某一特定波长激发光源的激发下,人体组织自体荧光光谱是多种内源性荧光物质光谱的叠加。 Under the excitation wavelength of the excitation light source of a particular human tissue autofluorescence spectroscopy is superimposed multiple endogenous fluorescent substance spectrum. 截至目前,已获得临床初步应用的自体荧光检测系统有(I)Xillix-LIFE lung/GI/ENT(Xillix Technologies Corp, Richmond, BC, Canada) ( WWik^ 442nm) ; (2) D-Iight System (Karl Storz, Tuttlingen, Germany) ( MR 'iA 442nm) ; (3)WavSTAT, Optical Biopsy System(SpectraScience, San Diego, USA)( # ^ ^ ^ 410nm) ; (4)SAFE-3000(Pentax, Tokyo, Japan) ( ■发M 长408nm) ; (5) Evis LuceraSpectrum(Olympus, Japan) Up to now, has received preliminary clinical application of autologous fluorescence detection system (I) Xillix-LIFE lung / GI / ENT (Xillix Technologies Corp, Richmond, BC, Canada) (WWik ^ 442nm); (2) D-Iight System ( Karl Storz, Tuttlingen, Germany) (MR 'iA 442nm); (3) WavSTAT, Optical Biopsy System (SpectraScience, San Diego, USA) (# ^ ^ ^ 410nm); (4) SAFE-3000 (Pentax, Tokyo, Japan ) (■ M long hair 408nm); (5) Evis LuceraSpectrum (Olympus, Japan)

长395-475nm)。 Long 395-475nm). 这些自体荧光内窥镜检测系统采用可见光波段的单一波长(408、410和442nm)或宽光谱激发光源(395-475nm),这些激发波长不仅不能激发色氨酸、胶原蛋白和还原型烟酰胺腺嘌呤二核苷酸的荧光,而且不能有效地激发卟啉的荧光。 These single wavelength autofluorescence endoscopy system uses visible light (408, 410 and 442nm) or broad spectrum excitation light source (395-475nm), the excitation wavelength is not only not stimulate tryptophan, collagen and reduced nicotinamide gland purine dinucleotide fluorescence, and can not effectively stimulate the porphyrin fluorescence. 因此,系统检测到的荧光信号主要来源于部分卟啉和黄素腺嘌呤二核苷酸。 Therefore, the system detects the fluorescence signal from the main part of the porphyrin and flavin adenine dinucleotide. 激发波长过于单一或者过于宽广均未能有效地激发具有诊断意义的荧光信息,在临床应用中尚无法实现人体早期肿瘤组织的高灵敏度和特异性检测。 Single excitation wavelength is too broad or too have failed to effectively stimulate fluorescence information with diagnostic significance in clinical applications still can not achieve high sensitivity and specificity of early detection of human tumor tissue.

发明内容 SUMMARY

[0003] 针对现有的人体组织自体荧光检测系统采用单一波长或者宽光谱光源作为激发光源,未能有效地激发具有诊断意义的内源性荧光物质的荧光信息,在临床应用中存在灵敏度和特异性不高等不足,本发明提供一种基于多波长光源激发的人体组织自体荧光检测系统,该系统可采用多个最佳激发波长激发人体组织中特定的内源性荧光物质,检测相应的荧光信息,避免采用单一波长或宽光谱光源激发导致荧光信息不够丰富或信息重叠不利于分析等问题,从而提高人体早期肿瘤组织的检测灵敏度和特异性。 [0003] The use of a single wavelength or broad spectrum light source for existing human tissue autofluorescence detection system as the excitation source, failed to effectively stimulate the intrinsic fluorescence of the fluorescent material information has diagnostic significance, there is a sensitivity and specificity in clinical applications of not higher insufficient, the present invention provides a multi-wavelength light source based on tissue autofluorescence excitation detection system, which can be a plurality of the optimum excitation wavelength of the excitation human tissue specific endogenous fluorescent substance, detection of the respective fluorescent information avoid the use of a single wavelength or broad spectrum fluorescent light excitation leads to duplication of information or information that is not rich enough to facilitate analysis of such issues, thereby enhancing the body of early tumor detection sensitivity and specificity.

[0004] 为实现本发明的目的采用的技术方案是:自体荧光检测系统由激发光源部分、激发-检测-采集部分、光学收集部分、信号预处理部分和计算机部分构成,其中信号预处理部分与计算机部分通过信号线进行连接,激发光源部分通过激发光与激发-检测-采集部分中的激发光输入端的光纤相连,激发-检测-采集部分中的荧光输出端的光纤通过荧光信号通路与光学收集部分相连,荧光信号通路经过光学收集部分传输后进入信号预处理部分,信号预处理部分中光电倍增管光电转化后变为电信号,进入计算机部分。 [0004] For the purpose of the use of technical solutions of the present invention are: autofluorescence detection system from the excitation light source section, excitation - detection - acquisition part, an optical collection portion, a signal preprocessing and computer parts, and wherein the signal pre-processing section Computer parts connected by a signal line, the excitation light source part by the excitation light and excitation - detection - Acquisition section of the excitation light input fiber connected to inspire - detection - acquisition part of the fluorescent output of the optical fiber through the fluorescent signal pathway and optical collection section connected fluorescence signal path after entering the optical collection portion of the transmission signal preprocessing, signal preprocessing section photomultiplier tube after photoelectric conversion into electrical signals into computer parts.

[0005] 所述的激发光源部分由波长可调谐激光器、光衰减器和耦合透镜组成,构成激发光通路。 An excitation light source part [0005], wherein the wavelength tunable laser, optical attenuator and the coupling lens, forming the excitation light path. 所述的可调谐激光器可以根据人体组织中特定的内源性荧光物质调节所需的脉冲激光波长,可依次调节的波长范围从210nm到2200nm,克服了现有的人体组织自体荧光检测系统采用单一波长或者宽光谱光源作为激发光源,未能有效地激发具有诊断意义的内源性荧光物质的荧光信息,在临床应用中存在灵敏度和特异性不高等不足;可调谐激光器输出的脉冲激光功率可以通过光衰减器进行调节,调节后的激发光经过耦合透镜耦合进入Y 型光纤的激发光输入端的光纤内。 The tunable laser can be adjusted according to the desired pulse laser wavelength tissue specific endogenous fluorescent substance, may in turn regulate the wavelength range from 210nm to 2200nm, to overcome the existing human tissue autofluorescence detection system uses a single or a wide-spectrum light source wavelength as the excitation source, failed to excite the fluorescence information effectively endogenous fluorescent substances have diagnostic significance, sensitivity, and specificity is not high shortcomings in clinical application; tunable laser output pulse laser power can be optical attenuator is adjusted, the adjusted excitation light via the coupling lens excitation light coupled into the input end of the Y-fiber.

[0006] 所述的激发_检测_采集部分由两路呈Y型的光纤和内窥镜组成,其中Y型光纤的一路由六根传输激发光的光纤,另一路为单根传输荧光光纤的荧光输出端。 _ _ Collection inspired detection section [0006], wherein the two Y-shaped fiber and endoscopes, of which Y-type optical fiber all the way to the excitation light transmitted by six, and the other way for a single transmission fluorescence optical fluorescence output. 传输激发光的光纤一端为激发光输入端,另一端为接入端,通过内窥镜的活检通道接入内窥镜内,激发光源部分发出的激发光通过该光纤照射待测组织;传输荧光的光纤一端为荧光输出端,另一端亦为接入端,通过内窥镜的活检通道接入内窥镜内。 One end of the optical fiber transmission excitation light for excitation light input end and the other end is the access side, through the biopsy channel of the endoscope access endoscopy, part of the excitation light source excitation light emitted by the fiber under test tissue irradiation; transmission fluorescence One end of the fiber optic fluorescence output end and the other end is also the access side, access through the endoscope biopsy channel of an endoscope. 传输激发光的光纤接入端和传输荧光光纤的接入端合并构成Y字型的尾部,传输激发光的光纤输入端和传输荧光光纤的输出端分开构成Y字型的两个头部。 Access end fiber access terminal and transmission fluorescence excitation light transmission fiber merger constitutes a Y-shaped tail, the transmission of the excitation light input and output ends of the optical fiber transmission fluorescent fibers constitute separate the two Y-shaped head. 待测组织发出的自体荧光通过该光纤的荧光输出端向光学收集部分传送荧光。 Measured tissue autofluorescence emitted fluorescence transmitted through the fluorescence optical collection portion output end of the optical fiber. 这样构成“激发光-光纤-内窥镜-照射-激发荧光”和“收集荧光-光纤-传出荧光”光通路。 This constitutes a "excitation light - Fiber - Endoscope - irradiation - fluorescence excitation" and "collect fluorescence - fiber - Outgoing fluorescent" light path.

[0007] 所述的光学收集部分由滤光轮和两个耦合透镜组成,传输荧光的光纤荧光输出端输出的荧光,通过前耦合透镜耦合后经过滤光轮中与激发光波长相对应的一个滤光片,再由后耦合透镜耦合输入信号预处理部分。 [0007] The optical collection portion by the filter wheel and two coupling lenses, optical fiber fluorescence output end of the transmission output fluorescence by coupling through the coupling lens front filter wheel with an appearance corresponding to the excitation wavelength filter rays, and then by the coupling lens coupled input signal after preprocessing. 滤光轮采用市场上可购买的通用型多孔滤光轮, 孔中根据对应的波长安装有不同中心波长的窄带滤光片。 The filter wheel can be purchased on the market using general-porous filter wheel wells fitted with different center wavelength of narrowband filter according to the corresponding wavelength. 当滤光轮上安装6个窄带滤光片时,中心波长可分别选择335nm,390nm,460nm,520nm,535nm,和635nm,带宽均为20nm,用于分别提取280nm激发光激发产生335nm的荧光信号,340nm激发光激发产生390和460nm的荧光信号,460nm激发光激发产生520nm的荧光信号,450nm激发光激发产生535nm的荧光信号和400nm激发光激发产生635nm的荧光信号。 When installing six narrowband filter on the filter wheel, the center wavelength can be selected 335nm, 390nm, 460nm, 520nm, 535nm, and 635nm, bandwidth are 20nm, 280nm excitation light for excitation were extracted to generate a fluorescent signal 335nm , 340nm excitation light generating fluorescence excitation signal of 390 and 460nm, 460nm excitation light to generate fluorescence signal of 520nm, 450nm excitation light to generate fluorescence signal 535nm and 400nm excitation light to generate fluorescence signal of 635nm. 其中,波长可调谐激光器和滤光轮由计算机部分同步控制,实现激发光波长与滤光轮上的滤光片的一一对应。 Among them, the wavelength tunable laser and filter wheel is controlled by the computer section synchronized to achieve optical wavelength excitation filter and filter wheel on the correspondence.

[0008] 所述的信号预处理部分由光电倍增管、前置放大器、光子计数器和计数板卡组成, 进入光电倍增管的光信号经过光电转化后变为电信号,依次经过前置放大器、光子计数器和计数板卡,进入计算机部分进行数据采集和处理,构成信号预处理部分。 [0008] The signal preprocessing section by a photomultiplier tube, a preamplifier, and a photon counter counts the board composition, into the photomultiplier tube of the optical signal after photoelectric conversion into electrical signals, passes through the pre-amplifier, the photon counter and count cards into the computer for data acquisition and processing part, constitute a signal preprocessing.

[0009] 本发明专利的有益效果是,采用波长可调谐的激光器作为激发光源,获取针对特定内源性荧光物质的最佳激发波长,同时检测相对应的发射荧光,实现了荧光信息的最佳激发和检测;同时,采用滤光片从连续荧光光谱中分解获取分立的荧光波长,这在硬件上实现从连续光谱分解出能够明确表征组织荧光特性的荧光信号,简化数据处理的过程,实现人体组织自体荧光的高灵敏度和特异性检测。 Best [0009] The present invention patents beneficial effects, using a wavelength tunable laser as the excitation source, to obtain optimal excitation wavelength for a specific endogenous fluorescent substance, simultaneous detection of fluorescence emission corresponding to achieve the fluorescence information excitation and detection; Meanwhile, from the continuous use of filter decomposition discrete fluorescence spectra to obtain a fluorescence wavelength, which from a continuous spectrum decomposition of fluorescence characteristics can be clearly characterizing the tissue fluorescence signal in hardware, simplifying the process of data processing, to achieve the body organizations with high sensitivity and specificity for detection of autofluorescence.

附图说明 Brief Description

[0010] 图1是本发明系统结构示意图。 [0010] Figure 1 is a schematic structural view of the system of the present invention. [0011] 图1中,1为可调谐激光器,2为光衰减器,3为耦合透镜,4为传输激发光的光纤一端的激发光输入端,5为传输激发光光纤接入内窥镜的另一端,6为内窥镜,7为待测组织,8 为传输荧光的荧光输出端,9为前耦合透镜,10为滤光轮,11为后耦合透镜,12为冷却电压控制器,13为冷却器,14为光电倍增管,15为前置放大器,16为单光子计数器,17为计数板卡,18为计算机。 [0011] Figure 1, 1 is a tunable laser, an optical attenuator for 2, 3 for the coupling lens, 4 an end of an optical fiber for transmitting the excitation light of the excitation light input terminal 5 to transmit the excitation light fiber access endoscope The other end, for the endoscope 6, 7 under test organization, the output end of the transmission 8 is a fluorescent phosphor, and 9 for the first coupling lens, filter wheel 10, after the coupling lens 11, a voltage controller 12 for cooling, 13 is cooler, photomultiplier tube 14, 15 is a preamplifier, 16 is a single-photon counter for counting boards 17, 18 for the computer.

具体实施方式 DETAILED DESCRIPTION

[0012] 以下结合附图对发明作进一步的说明。 [0012] THE DRAWINGS The invention will further be described.

[0013] 实施例1 [0013] Example 1

[0014] 如图1所示,基于不同波长激发的自体荧光检测系统图。 Shown in [0014] Figure 1, Figure autofluorescence detection system based on different excitation wavelengths. 选取一例人体结肠组织进行自体荧光光谱特性研究。 Select case study of human colon tissue fluorescence spectral characteristics of autologous. 在进行实验之前,通过冷却电压控制器(12)将冷却器(13) (C9144, Hamamatsu Corp.,Japan)开启并冷却30 分钟,使光电倍增管(14) (R928, Hamamatsu Corp.,Hamamatsu, Japan)达到稳定工作状态。 Before the experiment, (12) the cooler (13) (C9144, Hamamatsu Corp., Japan) open voltage controller and cooled by cooling for 30 minutes to a photomultiplier tube (14) (R928, Hamamatsu Corp., Hamamatsu, Japan) to reach steady state. 根据实验要求,打开计算机(18),并预先存储了与结肠组织相对应的三个阈值In和IT2,其数值分别为30000和3,打开可调谐激光器(1) (PIV OPO 355,0Ρ0ΤΕΚ, USA),通过计算机(18)上的控制软件调节可调谐激光器(1)的输出波长为400nm,同时控制选择滤光轮(10) (FW102B, Thorlabs Inc., USA)上中心波长为635nm的滤光片。 According to the experimental requirements, turn on the computer (18), and stored with the corresponding three colonic tissue threshold In and IT2, its value is 30,000, and 3, respectively, open tunable lasers (1) (PIV OPO 355,0Ρ0ΤΕΚ, USA ), by controlling the computer software (18) on the adjusting tunable laser (1) of the output wavelength of 400nm, while controlling the selection filter wheel (10) (FW102B, Thorlabs Inc., USA) on a central wavelength of 635nm filter tablets. 可调谐激光器(1)发出的激发光经过光衰减器(2) 进行功率调节,之后经过耦合透镜(3)耦合并进入传输激发光光纤的激发光输入端(4)上的六根传输激发光的光纤。 Excitation tunable laser (1) light emitted through the optical attenuator (2) for power regulation, after the coupling lens (3) is coupled into the excitation and the excitation light transmission optical fiber transmission six input terminal (4) on the excitation light optical fiber. 传输激发光的光纤接入端(5)通过内窥镜(6) (CF-1T140I/L, Olympus Corp.,Japan)将激发光通过活检通道均勻照射在待测组织(7)上,待测组织(7) 被激发后发出的荧光再由Y型光纤中的传输荧光的光纤接入端收集,最后从Y型光纤的荧光输出端(8)输出。 Transmission of the excitation light fiber access terminal (5) via an endoscope (6) (CF-1T140I / L, Olympus Corp., Japan) the excitation light is irradiated uniformly through the biopsy channel in the test tissue (7), measured Organization (7) after being excited fluorescence emitted by another fiber of Y-type fiber access terminal transmission fluorescence collected, and finally the output ends of the Y from the fluorescent optical fiber (8) output. 输出的荧光由前耦合透镜(9)耦合后经过滤光轮(10)上中心波长为635nm的滤光片,进行窄带滤波后,再由后耦合透镜(11)耦合到光电倍增管(14),通过光电倍增管(14)光电转化后转换为电信号,经过前置放大器(15) (C6438,Hamamatsu Corp., Hamamatsu, Japan)进行电信号放大,放大后的电信号再由单光子计数器(16) (C9744, Hamamatsu Corp. , Hamamatsu, Japan)鉴另U后通过计数板卡(17) (M8784, Hamamatsu Corp., Hamamatsu, Japan)进行计数并由计算机(18)记录635nm的荧光信号I635,其数值为10000, 小于阈值IT1,未能判定组织类型,须进行进一步检测。 Fluorescence output from the front coupling lens (9) is coupled through a filter wheel (10) on the filter center wavelength of 635nm, after narrowband filtering, then (11) is coupled by the coupling lens after the photomultiplier tube (14) , converted by the photomultiplier tube (14) photoelectric conversion into electrical signals, via a preamplifier (15) (C6438, Hamamatsu Corp., Hamamatsu, Japan) were amplified electric signal, and then the electric signal amplified by the single photon counter ( 16) (C9744, Hamamatsu Corp., Hamamatsu, Japan) were counted after Kam another U by computer (18) recording the fluorescence signal I635 635nm by counting board (17) (M8784, Hamamatsu Corp., Hamamatsu, Japan), a value of 10 000, is less than the threshold value IT1, failure determining tissue type, to carry out further tests. 再通过计算机(18)上的控制软件调节可调谐激光器(1)的输出波长为280nm,同时控制选择中心波长为335nm的滤光片,由计数板卡(17)进行计数并由计算机(18)记录335nm的荧光信号I335,其数值为96000 ;再通过计算机(18)上的控制软件调节可调谐激光器(1)的输出波长为340nm,同时控制选择中心波长为390nm的滤光片,由计数板卡(17)进行计数并由计算机(18)记录390nm的荧光信号I39。 Then adjust the tunable laser by controlling the computer software (18) (1) of the output wavelength of 280nm, while controlling the selection of a center wavelength of 335nm filter, by counting board (17) is counted by the computer (18) recording 335nm fluorescence signal I335, a value of 96,000; computer through the control software (18) on the adjusting tunable laser (1) of the output wavelength of 340nm, while controlling the selection of a center wavelength of 390nm filter, by counting board Card (17) counted by the computer (18) recording the fluorescence signal I39 390nm. ,其数值为50000 ;再选择中心波长为460nm的滤光片,记录460nm的荧光信号146(1, 其数值为82000。再选择可调谐激光器(1)输出波长为450nm,同时选择中心波长为535nm 的滤光片检测535nm的荧光信号I535,其数值为15000。最后,再选择可调谐激光器(1)输出波长为460nm,同时选择中心波长为520nm的滤光片检测520nm的荧光信号152(1,其数值为20000。在计算机(18)上进行数据处理,计算Ir = (I46O+I52O+I535)XI335Z(I39O)2的结果为4. 49,大于阈值It2,表明还原型烟酰胺腺嘌呤二核苷酸,黄素腺嘌呤二核苷酸和色氨酸相对于胶原蛋白的相对含量较高,判定待测组织为结肠肿瘤组织。[0015] 实施例2 , A value of 50,000; then select a center wavelength of 460nm filter, record 460nm fluorescence signal 146 (a, a value of 82000. reselection tunable laser (1) output wavelength 450nm, while selecting the center wavelength of 535nm filter detecting fluorescence signal I535 535nm and has a value of 15000. Finally, select tunable laser (1) output wavelength 460nm, while selecting the filter central wavelength 520nm 520nm fluorescence detection signal 152 (1, Its value is 20000. On the computer (18) for data processing, computing Ir = (I46O + I52O + I535) XI335Z (I39O) Results for 4.49, greater than the threshold It2, showed reduced nicotinamide adenine dinucleotide nuclear nucleotide, flavin adenine dinucleotide and tryptophan with relatively high content of collagen tissue is judged to be tested for colon cancer tissue. [0015] Example 2

[0016] 选取另一例人体结肠组织进行自体荧光光谱特性研究。 [0016] Another example choose to study human colon tissue fluorescence spectral characteristics of autologous. 按照实施例1的过程,首先选择400nm激发光,检测635nm的荧光信号I635,其记录的数值为9000,小于阈值IT1,未能判定组织类型,须进行进一步检测。 According to the procedure of Example 1, first select the 400nm excitation light, detecting the fluorescent signal I635 635nm, the value recorded for 9000, less than the threshold value IT1, failure determining tissue type, to carry out further tests. 接着,选择280nm激发光激发,检测335nm的荧光信号I335,其数值为75000。 Next, select the 280nm excitation light, detecting the fluorescent signal I335 335nm and has a value of 75,000. 然后选择340nm激发光,检测390nm和460nm的荧光信号I39tl和146(1, 其数值分别为70000和81000。再选择450nm激发光激发,检测535nm的荧光信号I535,其数值为10000。最后,选择460nm激发光,检测520的荧光信号I52tl,其数值为18000。计算Ir = (I46O+I52O+I535)XI335Z(I39O)2的结果为I- 67,小于阈值IT2,表明表明还原型烟酰胺腺嘌呤二核苷酸,黄素腺嘌呤二核苷酸和色氨酸相对于胶原蛋白的相对含量较低,判定待测组织为结肠正常组织。 Then select the 340nm excitation light, detecting the fluorescent signal I39tl 460nm and 390nm and 146 (1, its value was 70000 and 81000. reselection 450nm excitation light, detecting the fluorescent signal I535 535nm and has a value of 10000. Finally, select 460nm excitation light, detecting the fluorescent signal I52tl 520 and has a value of 18000. calculated Ir = (I46O + I52O + I535) XI335Z (I39O) Results of I- 67, less than the threshold value IT2, show show reduced nicotinamide adenine dinucleotide nucleotides, flavin adenine dinucleotide, and tryptophan with respect to the relatively low collagen content, it is determined as normal colon tissues tested tissue.

[0017] 实施例3 [0017] Example 3

[0018] 选取另一例人体结肠组织进行自体荧光光谱特性研究。 [0018] Another example choose to study human colon tissue fluorescence spectral characteristics of autologous. 按照实施例1的过程,首先选择400nm激发光,检测635nm的荧光信号I635,其记录的数值为60000,高于阈值In,表明卟啉含量较高,判定待测组织为结肠肿瘤组织。 Following the procedure of Example 1, first select the 400nm excitation light, detecting the fluorescent signal I635 635nm, values which record 60000, higher than the threshold value In, indicating a high porphyrin content, determined as the colon tumor tissues tested tissue.

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