CN101918527A - 富集和改变细胞和其他微粒的装置和方法 - Google Patents
富集和改变细胞和其他微粒的装置和方法 Download PDFInfo
- Publication number
- CN101918527A CN101918527A CN2006800198868A CN200680019886A CN101918527A CN 101918527 A CN101918527 A CN 101918527A CN 2006800198868 A CN2006800198868 A CN 2006800198868A CN 200680019886 A CN200680019886 A CN 200680019886A CN 101918527 A CN101918527 A CN 101918527A
- Authority
- CN
- China
- Prior art keywords
- cell
- sample
- size
- particulate
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
- B03C1/30—Combinations with other devices, not otherwise provided for
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/32—Magnetic separation acting on the medium containing the substance being separated, e.g. magneto-gravimetric-, magnetohydrostatic-, or magnetohydrodynamic separation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/0005—Field flow fractionation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0472—Diffusion
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/08—Regulating or influencing the flow resistance
- B01L2400/084—Passive control of flow resistance
- B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C2201/00—Details of magnetic or electrostatic separation
- B03C2201/18—Magnetic separation whereby the particles are suspended in a liquid
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y80/00—Products made by additive manufacturing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00178—Special arrangements of analysers
- G01N2035/00237—Handling microquantities of analyte, e.g. microvalves, capillary networks
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T137/00—Fluid handling
- Y10T137/0318—Processes
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T137/00—Fluid handling
- Y10T137/8593—Systems
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
Abstract
Description
样品编号 | 流量 | 执行结果 | ||
RBC移除 | 血小板移除 | WBC损失 |
12345 | 4mL/hr6mL/hr6mL/hr6mL/hr6mL/hr | 100%100%100%100%100% | 99%99%99%97%98% | <1%<1%<1%<1%<1% |
阵列设计: | L5 |
间隙尺寸: | 第一段:22μm |
液流角: | 1/50 |
阵列/芯片: | 48 |
标称深度 | 150μm |
装置底面积 | 32mm×64mm |
设计特征 | ·多路单阵列·最优化的旁路通道·液流稳定·液流导入和液流导出边界 |
处理的体积(mL) | 26.5 | 8 | 15.4 | 17 | 19 |
流量(mL/h) | 10.6 | 10.0 | 11.8 | 9.8 | 9.8 |
废弃物中的WBC/输入的WBC(Nageotte) | 0.013% | 0.012% | 0 005% | 0.014% | 0.030% |
RBC去除 | 99.993 | 99.992 | 99.997 | 99.995 | 99.999 |
血小板去除 | >99.6% | >99.7% | >99.7% | >99.7% | >99.7% |
裂解前 | 裂解后 | 细胞回收% | |
eRBCsnRBCsfnRBCs | 2.6×106426 | 0.03×106264 | 1%62%68% |
样品 | 起始细胞计数 | 最终细胞计数 | 残留% |
1未处理 | 6.6×106 | 6.6×106 | 100% |
210秒接触 | 6.6×106 | 7.2×105 | 10.9% |
320秒接触 | 6.6×106 | 4.6×105 | 6.9% |
430秒接触 | 6.6×106 | 3.4×105 | 5.2% |
Claims (168)
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US66841505P | 2005-04-05 | 2005-04-05 | |
US60/668,415 | 2005-04-05 | ||
US70406705P | 2005-07-29 | 2005-07-29 | |
US60/704,067 | 2005-07-29 | ||
PCT/US2006/012820 WO2006108101A2 (en) | 2005-04-05 | 2006-04-05 | Devices and method for enrichment and alteration of cells and other particles |
Publications (2)
Publication Number | Publication Date |
---|---|
CN101918527A true CN101918527A (zh) | 2010-12-15 |
CN101918527B CN101918527B (zh) | 2012-06-27 |
Family
ID=37071048
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN2006800198868A Active CN101918527B (zh) | 2005-04-05 | 2006-04-05 | 富集和改变细胞和其他微粒的装置和方法 |
Country Status (9)
Country | Link |
---|---|
US (7) | US20070196820A1 (zh) |
EP (3) | EP2492339B1 (zh) |
JP (1) | JP2008538283A (zh) |
CN (1) | CN101918527B (zh) |
AU (1) | AU2006232103A1 (zh) |
CA (1) | CA2601480A1 (zh) |
GB (1) | GB2429774A (zh) |
HK (1) | HK1175494A1 (zh) |
WO (1) | WO2006108101A2 (zh) |
Cited By (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104212708A (zh) * | 2014-09-19 | 2014-12-17 | 张蓉 | 一种肠道内容物目标成分提取装置 |
CN105431537A (zh) * | 2013-08-07 | 2016-03-23 | 罗伯特·博世有限公司 | 用于处理包含靶细胞和伴随细胞的生物材料样品以提取靶细胞的核酸的方法和装置 |
CN105473716A (zh) * | 2013-08-07 | 2016-04-06 | 罗伯特·博世有限公司 | 用于处理包含靶细胞和伴随细胞的生物材料样品以提取靶细胞的核酸的方法和装置 |
CN106119189A (zh) * | 2016-07-11 | 2016-11-16 | 山东亚大药业有限公司 | 胎儿有核红细胞的分离纯化方法及试剂盒 |
CN107206380A (zh) * | 2015-01-23 | 2017-09-26 | 和卓生物科技(上海)有限公司 | 用于非侵入性产前测试的基于微流体的胎儿细胞的检测和分离 |
CN107429262A (zh) * | 2015-01-07 | 2017-12-01 | 英迪公司 | 一种用于机械和水动力微流体转染的方法以及用于其的设备 |
CN107723207A (zh) * | 2017-11-01 | 2018-02-23 | 深圳市瑞格生物科技有限公司 | 一种分离捕获细胞的芯片及其在肿瘤细胞分选中的应用 |
CN108495922A (zh) * | 2016-01-22 | 2018-09-04 | 小利兰·斯坦福大学托管委员会 | 一种用于从未处理的精液中选择性分选高活动和形态正常的精子的微流体装置 |
CN109311023A (zh) * | 2016-04-22 | 2019-02-05 | 普渡研究基金会 | 高通量颗粒捕获与分析 |
CN109562380A (zh) * | 2016-08-02 | 2019-04-02 | Imec 非营利协会 | 用于聚集流中的物体的方法和装置 |
CN110186835A (zh) * | 2013-03-15 | 2019-08-30 | Gpb科学有限责任公司 | 颗粒的片上微流体处理 |
CN110799840A (zh) * | 2016-10-23 | 2020-02-14 | 伯克利之光生命科技公司 | 筛选b细胞淋巴细胞的方法 |
CN110862905A (zh) * | 2020-01-09 | 2020-03-06 | 北京航空航天大学合肥创新研究院 | 用于细胞迁移实验的芯片装置、制备方法及实验方法 |
CN110885742A (zh) * | 2018-05-25 | 2020-03-17 | 昆山汇先医药技术有限公司 | 富集筛选机构及其制备方法 |
CN111212900A (zh) * | 2017-10-03 | 2020-05-29 | Nok株式会社 | 细胞捕获装置 |
CN112912725A (zh) * | 2018-10-25 | 2021-06-04 | Tl基因体科技株式会社 | 使用微通道对血液进行预处理以对血细胞进行分级 |
CN114272966A (zh) * | 2015-07-22 | 2022-04-05 | 北卡罗来纳-查佩尔山大学 | 含有具有空间分离的珠保留和信号检测段的珠孔几何结构的流体装置和相关方法 |
Families Citing this family (193)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6692952B1 (en) * | 1999-11-10 | 2004-02-17 | Massachusetts Institute Of Technology | Cell analysis and sorting apparatus for manipulation of cells |
US6913697B2 (en) | 2001-02-14 | 2005-07-05 | Science & Technology Corporation @ Unm | Nanostructured separation and analysis devices for biological membranes |
JP2006501449A (ja) * | 2002-09-27 | 2006-01-12 | ザ ジェネラル ホスピタル コーポレーション | 細胞分離のためのマイクロ流体デバイスおよびその使用 |
US20040171091A1 (en) * | 2003-02-27 | 2004-09-02 | Cell Work, Inc. | Standardized evaluation of therapeutic efficacy based on cellular biomarkers |
EP1636564A1 (en) * | 2003-06-13 | 2006-03-22 | The General Hospital Corporation | Microfluidic systems for size based removal of red blood cells and platelets from blood |
US8158410B2 (en) | 2005-01-18 | 2012-04-17 | Biocept, Inc. | Recovery of rare cells using a microchannel apparatus with patterned posts |
EP1838442B1 (en) | 2005-01-18 | 2013-09-11 | Biocept, Inc. | Cell separation using microchannel having patterned posts |
US20090136982A1 (en) | 2005-01-18 | 2009-05-28 | Biocept, Inc. | Cell separation using microchannel having patterned posts |
US20060252087A1 (en) * | 2005-01-18 | 2006-11-09 | Biocept, Inc. | Recovery of rare cells using a microchannel apparatus with patterned posts |
US20070026413A1 (en) * | 2005-07-29 | 2007-02-01 | Mehmet Toner | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
US20060223178A1 (en) * | 2005-04-05 | 2006-10-05 | Tom Barber | Devices and methods for magnetic enrichment of cells and other particles |
US20070196820A1 (en) | 2005-04-05 | 2007-08-23 | Ravi Kapur | Devices and methods for enrichment and alteration of cells and other particles |
US20070026414A1 (en) * | 2005-07-29 | 2007-02-01 | Martin Fuchs | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
US20070026417A1 (en) * | 2005-07-29 | 2007-02-01 | Martin Fuchs | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
EP2594631A1 (en) * | 2005-04-05 | 2013-05-22 | Cellpoint Diagnostics | Devices and method for detecting circulating tumor cells and other particles |
US20070026415A1 (en) * | 2005-07-29 | 2007-02-01 | Martin Fuchs | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
US20090181421A1 (en) * | 2005-07-29 | 2009-07-16 | Ravi Kapur | Diagnosis of fetal abnormalities using nucleated red blood cells |
US20070059680A1 (en) * | 2005-09-15 | 2007-03-15 | Ravi Kapur | System for cell enrichment |
US8921102B2 (en) | 2005-07-29 | 2014-12-30 | Gpb Scientific, Llc | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
US20070026416A1 (en) * | 2005-07-29 | 2007-02-01 | Martin Fuchs | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
US20070059719A1 (en) * | 2005-09-15 | 2007-03-15 | Michael Grisham | Business methods for prenatal Diagnosis |
US20070059683A1 (en) * | 2005-09-15 | 2007-03-15 | Tom Barber | Veterinary diagnostic system |
US20070059774A1 (en) * | 2005-09-15 | 2007-03-15 | Michael Grisham | Kits for Prenatal Testing |
US20070059716A1 (en) * | 2005-09-15 | 2007-03-15 | Ulysses Balis | Methods for detecting fetal abnormality |
US20070059718A1 (en) * | 2005-09-15 | 2007-03-15 | Mehmet Toner | Systems and methods for enrichment of analytes |
US20070059781A1 (en) * | 2005-09-15 | 2007-03-15 | Ravi Kapur | System for size based separation and analysis |
US7695956B2 (en) * | 2006-01-12 | 2010-04-13 | Biocept, Inc. | Device for cell separation and analysis and method of using |
PT2385143T (pt) | 2006-02-02 | 2016-10-18 | Univ Leland Stanford Junior | Rastreio genético fetal não-invasivo por análise digital |
WO2007137245A2 (en) | 2006-05-22 | 2007-11-29 | Columbia University | Systems and methods of microfluidic membraneless exchange using filtration of extraction fluid outlet streams |
EP2029779A4 (en) | 2006-06-14 | 2010-01-20 | Living Microsystems Inc | HIGHLY PARALLEL SNP GENOTYPING UTILIZATION FOR FETAL DIAGNOSIS |
EP2589668A1 (en) | 2006-06-14 | 2013-05-08 | Verinata Health, Inc | Rare cell analysis using sample splitting and DNA tags |
US20080050739A1 (en) * | 2006-06-14 | 2008-02-28 | Roland Stoughton | Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats |
WO2007147018A1 (en) * | 2006-06-14 | 2007-12-21 | Cellpoint Diagnostics, Inc. | Analysis of rare cell-enriched samples |
US8137912B2 (en) | 2006-06-14 | 2012-03-20 | The General Hospital Corporation | Methods for the diagnosis of fetal abnormalities |
US20080007838A1 (en) * | 2006-07-07 | 2008-01-10 | Omnitech Partners, Inc. | Field-of-view indicator, and optical system and associated method employing the same |
US20100288689A1 (en) * | 2006-08-22 | 2010-11-18 | Agency For Science, Technology And Research | Microfluidic filtration unit, device and methods thereof |
CN101765762B (zh) | 2007-04-16 | 2013-08-14 | 通用医疗公司以马萨诸塞州通用医疗公司名义经营 | 使粒子在微通道中聚集的系统和方法 |
US20100233694A1 (en) * | 2007-04-16 | 2010-09-16 | On-O-ity, Inc | Devices and methods for diagnosing, prognosing, or theranosing a condition by enriching rare cells |
BRPI0810095A2 (pt) * | 2007-04-20 | 2014-10-21 | Gen Hospital Corp | Método para a contagem de células |
EP3067807A1 (en) | 2007-07-23 | 2016-09-14 | The Chinese University of Hong Kong | Diagnosing fetal chromosomal aneuploidy using genomic sequencing |
US20100112590A1 (en) | 2007-07-23 | 2010-05-06 | The Chinese University Of Hong Kong | Diagnosing Fetal Chromosomal Aneuploidy Using Genomic Sequencing With Enrichment |
US20090053799A1 (en) * | 2007-08-23 | 2009-02-26 | Cynvenio Biosystems, Llc | Trapping magnetic sorting system for target species |
US20110300608A1 (en) * | 2007-09-21 | 2011-12-08 | Streck, Inc. | Nucleic acid isolation in preserved whole blood |
WO2009058997A2 (en) * | 2007-11-01 | 2009-05-07 | Biocept Inc. | Non-invasive isolation of fetal nucleic acid |
JP2011514182A (ja) * | 2008-02-04 | 2011-05-06 | ザ トラスティーズ オブ コロンビア ユニバーシティ イン ザ シティ オブ ニューヨーク | 流体分離装置、システム、及び方法 |
US8008032B2 (en) | 2008-02-25 | 2011-08-30 | Cellective Dx Corporation | Tagged ligands for enrichment of rare analytes from a mixed sample |
US20110127222A1 (en) * | 2008-03-19 | 2011-06-02 | Cynvenio Biosystems, Inc. | Trapping magnetic cell sorting system |
US20110137018A1 (en) * | 2008-04-16 | 2011-06-09 | Cynvenio Biosystems, Inc. | Magnetic separation system with pre and post processing modules |
FR2931141B1 (fr) | 2008-05-13 | 2011-07-01 | Commissariat Energie Atomique | Systeme microfluidique et procede pour le tri d'amas de cellules et de preference pour leur encapsulation en continu suite a leur tri |
FR2931085B1 (fr) | 2008-05-13 | 2011-05-27 | Commissariat Energie Atomique | Procede de tri de particules ou d'amas de particules dans un fluide circulant dans un canal |
WO2010006174A2 (en) | 2008-07-10 | 2010-01-14 | Reichenbach Steven H | Method and apparatus for sorting particles using asymmetrical particle shifting |
EP2315848B1 (en) * | 2008-07-18 | 2014-12-10 | Canon U.S. Life Sciences, Inc. | Methods and systems for microfluidic dna sample preparation |
US20110262916A1 (en) * | 2008-07-18 | 2011-10-27 | Wen-Hua Fan | Non-invasive fetal rhd genotyping from maternal whole blood |
EP2952589B1 (en) | 2008-09-20 | 2018-02-14 | The Board of Trustees of The Leland Stanford Junior University | Noninvasive diagnosis of fetal aneuploidy by sequencing |
IT1391408B1 (it) * | 2008-10-02 | 2011-12-23 | Silicon Biosystems Spa | Camera di separazione |
EP2350652A2 (en) * | 2008-10-10 | 2011-08-03 | Cnrs-Dae | Cell sorting device |
JP2010099052A (ja) * | 2008-10-27 | 2010-05-06 | Olympus Corp | 細胞分離方法 |
US8162149B1 (en) | 2009-01-21 | 2012-04-24 | Sandia Corporation | Particle sorter comprising a fluid displacer in a closed-loop fluid circuit |
JP5750661B2 (ja) * | 2009-01-23 | 2015-07-22 | 学校法人 芝浦工業大学 | 三次元誘電泳動デバイス |
US20100304978A1 (en) * | 2009-01-26 | 2010-12-02 | David Xingfei Deng | Methods and compositions for identifying a fetal cell |
AU2010239131A1 (en) * | 2009-04-21 | 2011-11-17 | Genetic Technologies Limited | Methods for obtaining fetal genetic material |
EP2437887B1 (en) | 2009-06-04 | 2016-05-11 | Lockheed Martin Corporation | Multiple-sample microfluidic chip for dna analysis |
CN102713640B (zh) * | 2009-06-10 | 2015-09-16 | 辛温尼奥生物系统公司 | 鞘流装置和方法 |
FR2946895A1 (fr) | 2009-06-19 | 2010-12-24 | Commissariat Energie Atomique | Systeme microfluidique et procede correspondant pour le transfert d'elements entre phases liquides et utilisation de ce systeme pour extraire ces elements |
WO2011025986A1 (en) * | 2009-08-28 | 2011-03-03 | The Trustees Of Columbia University In The City Of New York | Multi-layer blood component exchange devices, systems, and methods |
US8584535B2 (en) * | 2009-09-17 | 2013-11-19 | Innova Prep LLC | Liquid to liquid biological particle concentrator with disposable fluid path |
RU2012124051A (ru) | 2009-11-13 | 2013-12-20 | Бекман Каултер, Инк. | Системы и способы для обнаружения наличия биологического признака с применением кластеризации |
US9034658B2 (en) | 2009-11-23 | 2015-05-19 | The General Hospital Corporation | Microfluidic devices for the capture of biological sample components |
CA2785681C (en) | 2009-12-07 | 2019-03-19 | Yale University | Label-free cellular manipulation and sorting via biocompatible ferrofluids |
CN102791616B (zh) | 2009-12-23 | 2015-07-29 | 西托维拉公司 | 用于粒子过滤的系统和方法 |
US8187979B2 (en) * | 2009-12-23 | 2012-05-29 | Varian Semiconductor Equipment Associates, Inc. | Workpiece patterning with plasma sheath modulation |
EP2526183A4 (en) | 2010-01-21 | 2017-05-03 | Biocep Ltd. | Magnetic separation of rare cells |
EP2542661B1 (en) * | 2010-03-04 | 2020-04-22 | National University of Singapore | Detection of circulating tumor cells in a microfluidic sorter |
US8774488B2 (en) | 2010-03-11 | 2014-07-08 | Cellscape Corporation | Method and device for identification of nucleated red blood cells from a maternal blood sample |
US20110293558A1 (en) * | 2010-03-22 | 2011-12-01 | Massachusetts Institute Of Technology | Material properties of t cells and related methods and compositions |
JP5728778B2 (ja) * | 2010-06-01 | 2015-06-03 | 国立大学法人九州工業大学 | 解析装置及び解析装置の製造方法 |
US8590710B2 (en) * | 2010-06-10 | 2013-11-26 | Samsung Electronics Co., Ltd. | Target particles-separating device and method using multi-orifice flow fractionation channel |
EP2625292B1 (en) | 2010-10-07 | 2018-12-05 | The General Hospital Corporation | Biomarkers of cancer |
AU2011315951B2 (en) | 2010-10-15 | 2015-03-19 | Lockheed Martin Corporation | Micro fluidic optic design |
AU2015268583B2 (en) * | 2010-10-28 | 2017-06-15 | Yale University | Microfluidic Processing Of Target Species In Ferrofluids |
WO2012057878A1 (en) | 2010-10-28 | 2012-05-03 | Yale University | Microfluidic processing of target species in ferrofluids |
KR101768123B1 (ko) * | 2010-12-03 | 2017-08-16 | 삼성전자주식회사 | 수력학 필터, 이를 구비한 필터링 장치 및 이에 의한 필터링 방법 |
CA2826384C (en) * | 2011-02-04 | 2020-08-04 | The Trustees Of The University Of Pennsylvania | A method for detecting chromosome structure and gene expression simultaneously in single cells |
EP2490005A1 (en) * | 2011-02-18 | 2012-08-22 | Koninklijke Philips Electronics N.V. | Microfluidic resistance network and microfluidic device |
EP2490020A1 (en) * | 2011-02-18 | 2012-08-22 | Koninklijke Philips Electronics N.V. | Measurement chip, microfluidic device and method of measurement chip manufacture |
KR101882864B1 (ko) | 2011-06-24 | 2018-08-27 | 삼성전자주식회사 | 수력학 필터 유닛, 이를 포함하는 수력학 필터 및 이들을 사용하여 표적 물질을 필터링하는 방법 |
CN103998932B (zh) | 2011-06-29 | 2017-06-06 | 中央研究院 | 使用表面涂层对生物物质的捕获、纯化和释放 |
US10466160B2 (en) | 2011-08-01 | 2019-11-05 | Celsee Diagnostics, Inc. | System and method for retrieving and analyzing particles |
US9404864B2 (en) | 2013-03-13 | 2016-08-02 | Denovo Sciences, Inc. | System for imaging captured cells |
US9174216B2 (en) | 2013-03-13 | 2015-11-03 | DeNovo Science, Inc. | System for capturing and analyzing cells |
CA2842359A1 (en) | 2011-08-01 | 2013-02-07 | Denovo Sciences | Cell capture system and method of use |
CA2844056A1 (en) * | 2011-08-04 | 2013-02-07 | Sage Science, Inc. | Systems and methods for processing fluids |
EP2771685B1 (en) | 2011-10-24 | 2018-06-13 | The General Hospital Corporation | Biomarkers of cancer |
KR101933618B1 (ko) | 2011-11-29 | 2018-12-31 | 삼성전자주식회사 | 표적 물질 검출 및 분리 장치, 및 이를 이용한 표적 물질 검출 및 분리 방법 |
MX353212B (es) | 2011-12-07 | 2018-01-08 | Cytovera Inc | Metodo y dispositivo para el procesamiento de muestras. |
US9322054B2 (en) | 2012-02-22 | 2016-04-26 | Lockheed Martin Corporation | Microfluidic cartridge |
FR2987282B1 (fr) * | 2012-02-24 | 2017-12-29 | Fonds De L'espci Georges Charpak | Microcanal avec dispositif d'ouverture et/ou fermeture et/ou pompage |
WO2014046621A1 (en) | 2012-09-21 | 2014-03-27 | Massachusetts Institute Of Technology | Micro-fluidic device and uses thereof |
US9599590B2 (en) | 2012-10-12 | 2017-03-21 | Sage Science, Inc. | Side-eluting molecular fractionator |
US9846157B2 (en) | 2012-10-26 | 2017-12-19 | The Trustees Of The University Of Pennsylvania | Compositions, methods and microfluidics device for telomerase based in vitro diagnostic assays for detecting circulating tumor cells (CTC) |
US9494500B2 (en) | 2012-10-29 | 2016-11-15 | Academia Sinica | Collection and concentration system for biologic substance of interest and use thereof |
US9606102B2 (en) | 2013-01-26 | 2017-03-28 | Denovo Sciences, Inc. | System and method for capturing and analyzing cells |
US8934700B2 (en) * | 2013-03-04 | 2015-01-13 | Caliper Life Sciences, Inc. | High-throughput single-cell imaging, sorting, and isolation |
WO2014138154A1 (en) * | 2013-03-06 | 2014-09-12 | President And Fellows Of Harvard College | Devices and methods for forming relatively monodisperse droplets |
US9707562B2 (en) | 2013-03-13 | 2017-07-18 | Denovo Sciences, Inc. | System for capturing and analyzing cells |
USD758372S1 (en) * | 2013-03-13 | 2016-06-07 | Nagrastar Llc | Smart card interface |
US9888283B2 (en) | 2013-03-13 | 2018-02-06 | Nagrastar Llc | Systems and methods for performing transport I/O |
US20150064153A1 (en) | 2013-03-15 | 2015-03-05 | The Trustees Of Princeton University | High efficiency microfluidic purification of stem cells to improve transplants |
WO2014144782A2 (en) | 2013-03-15 | 2014-09-18 | Ancera, Inc. | Systems and methods for active particle separation |
WO2016019393A1 (en) | 2014-08-01 | 2016-02-04 | Gpb Scientific, Llc | Methods and systems for processing particles |
EP2971279B1 (en) | 2013-03-15 | 2019-11-13 | The Trustees of Princeton University | Methods and devices for high throughput purification |
WO2014145765A1 (en) | 2013-03-15 | 2014-09-18 | Ancera, Inc. | Systems and methods for bead-based assays in ferrofluids |
WO2014145237A1 (en) * | 2013-03-15 | 2014-09-18 | Dialyflux, Llc | Surfaces for manipulating particle flow |
US9856535B2 (en) | 2013-05-31 | 2018-01-02 | Denovo Sciences, Inc. | System for isolating cells |
US10391490B2 (en) | 2013-05-31 | 2019-08-27 | Celsee Diagnostics, Inc. | System and method for isolating and analyzing cells |
US9656262B2 (en) * | 2013-06-11 | 2017-05-23 | Euveda Biosciences, Inc. | Microfluidic grid-based design for high throughput assays |
EP3058316B1 (en) | 2013-10-18 | 2019-03-13 | The General Hospital Corporation | Microfluidic sorting using high gradient magnetic fields |
US9909102B2 (en) * | 2013-11-19 | 2018-03-06 | Platod | Fluidic device for producing platelets |
US20160279637A1 (en) | 2013-11-22 | 2016-09-29 | The General Hospital Corporation | Microfluidic methods and systems for isolating particle clusters |
US10047344B2 (en) | 2014-02-18 | 2018-08-14 | National University Of Singapore | Biophysically sorted osteoprogenitors from culture expanded bone marrow derived mesenchymal stromal cells (MSCs) |
JP2015166707A (ja) * | 2014-03-04 | 2015-09-24 | キヤノン株式会社 | マイクロ流路デバイス |
EP3126814B1 (en) | 2014-04-01 | 2019-06-12 | Academia Sinica | Methods and systems for cancer diagnosis and prognosis |
CN106459863A (zh) | 2014-05-01 | 2017-02-22 | 阿卜杜拉国王科技大学 | 分离细胞的微流体装置 |
CN106796164B (zh) * | 2014-08-07 | 2020-12-29 | 通用医疗公司 | 细胞的血小板靶向微流体分离 |
KR102323205B1 (ko) | 2014-08-22 | 2021-11-08 | 삼성전자주식회사 | 표적물질 분리장치 및 표적물질 분리방법 |
CN105381824B (zh) | 2014-08-26 | 2019-04-23 | 中央研究院 | 收集器架构布局设计 |
WO2016044555A1 (en) | 2014-09-17 | 2016-03-24 | Massachusetts Institute Of Technology | System and method for inertial focusing microfiltration for intra-operative blood salvage autotransfusion |
MX2017004507A (es) | 2014-10-06 | 2017-06-28 | Univ Pennsylvania | Composiciones y metodos para el aislamiento de celulas tumorales circulantes (ctc). |
CA2964487C (en) | 2014-10-15 | 2024-03-19 | Sage Science, Inc. | Apparatuses, methods and systems for automated processing of nucleic acids and electrophoretic sample preparation |
WO2016073486A1 (en) | 2014-11-03 | 2016-05-12 | The General Hospital Corporation | Concentrating particles in a microfluidic device |
US9636675B2 (en) * | 2014-11-26 | 2017-05-02 | International Business Machines Corporation | Pillar array structure with uniform and high aspect ratio nanometer gaps |
US9835538B2 (en) * | 2014-11-26 | 2017-12-05 | International Business Machines Corporation | Biopolymer separation using nanostructured arrays |
KR102360072B1 (ko) * | 2014-12-08 | 2022-02-08 | 삼성전자주식회사 | 미세입자 분리 장치 |
KR101583633B1 (ko) * | 2015-01-12 | 2016-01-08 | 한국항공대학교산학협력단 | 음의 유전 영동력 기반의 입자 분리 장치 및 이를 이용한 입자 분리 방법 |
US10364467B2 (en) | 2015-01-13 | 2019-07-30 | The Chinese University Of Hong Kong | Using size and number aberrations in plasma DNA for detecting cancer |
KR101749796B1 (ko) * | 2015-02-24 | 2017-06-22 | 고려대학교 산학협력단 | 혈소판 검사용 칩 |
JPWO2016136273A1 (ja) * | 2015-02-27 | 2017-12-07 | 凸版印刷株式会社 | 細胞を分離する方法、およびそのためのデバイス |
US10156568B2 (en) | 2015-04-30 | 2018-12-18 | International Business Machines Corporation | Immunoassay for detection of virus-antibody nanocomplexes in solution by chip-based pillar array |
USD864968S1 (en) | 2015-04-30 | 2019-10-29 | Echostar Technologies L.L.C. | Smart card interface |
GB2598499B (en) | 2015-06-05 | 2022-05-25 | Novartis Ag | Flow-through paramagnetic particle-based cell separation and paramagnetic particle removal |
WO2016210348A2 (en) | 2015-06-26 | 2016-12-29 | Ancera, Inc. | Background defocusing and clearing in ferrofluid-based capture assays |
US10976232B2 (en) | 2015-08-24 | 2021-04-13 | Gpb Scientific, Inc. | Methods and devices for multi-step cell purification and concentration |
CN108699101B (zh) | 2015-11-20 | 2022-04-19 | 塞奇科学股份有限公司 | 用于基因组dna片段的靶向纯化的制备型电泳方法 |
US10107726B2 (en) | 2016-03-16 | 2018-10-23 | Cellmax, Ltd. | Collection of suspended cells using a transferable membrane |
WO2017221898A1 (ja) * | 2016-06-20 | 2017-12-28 | 凸版印刷株式会社 | 液体媒体の置換方法及び該方法のための流路デバイス |
CN106053456B (zh) * | 2016-06-24 | 2018-11-27 | 许毅 | 一种尿液浓缩装置 |
CN109952106B (zh) | 2016-07-21 | 2022-08-05 | 伯克利之光生命科技公司 | 在微流体装置中分选t淋巴细胞 |
JP6843420B2 (ja) * | 2016-08-22 | 2021-03-17 | 国立大学法人東京工業大学 | 微粒子分離デバイスおよび微粒子の分離方法 |
EP3957997A1 (en) * | 2016-08-26 | 2022-02-23 | Juno Therapeutics, Inc. | Methods of enumerating particles present in a cell composition |
CN110520206B (zh) * | 2016-09-13 | 2022-03-18 | 海世欧申有限责任公司 | 微流体过滤装置 |
US10386276B2 (en) | 2016-09-20 | 2019-08-20 | International Business Machines Corporation | Phosphoprotein detection using a chip-based pillar array |
US10253350B2 (en) * | 2016-09-20 | 2019-04-09 | International Business Machines Corporation | Separation of molecules using nanopillar arrays |
US10010883B2 (en) | 2016-09-20 | 2018-07-03 | International Business Machines Corporation | Deterministic lateral displacement arrays |
WO2018085453A1 (en) | 2016-11-01 | 2018-05-11 | California Institute Of Technology | Microfluidic devices and methods for purifying rare antigen-specific t cell populations |
US10603647B2 (en) * | 2016-12-01 | 2020-03-31 | Imagine Tf, Llc | Microstructure flow mixing devices |
US10471425B2 (en) | 2017-02-16 | 2019-11-12 | International Business Machines Corporation | Automated machine for sorting of biological fluids |
JP2020520787A (ja) | 2017-03-28 | 2020-07-16 | クロマタン インコーポレイテッド | 連続向流スパイラルクロマトグラフィー |
CN110506203A (zh) | 2017-04-07 | 2019-11-26 | 塞奇科学股份有限公司 | 用于通过使用集成电泳dna纯化来检测遗传结构变异的系统和方法 |
DE102017003455B4 (de) * | 2017-04-10 | 2020-12-10 | Forschungszentrum Jülich GmbH | Vorrichtung und Verfahren zur Umwandlung von Wärme, chemischer Energie oder elektrischer Energie in Bewegungsenergie sowie Verwendung der Vorrichtung |
JP6990713B2 (ja) | 2017-04-14 | 2022-01-12 | ヴェンタナ メディカル システムズ, インク. | 解離した固定組織のサイズに基づいた分離 |
US11648559B2 (en) * | 2017-08-04 | 2023-05-16 | University Of Georgia Research Foundation, Inc. | Devices and methods for separating circulating tumor cells from biological samples |
EP3651903A4 (en) | 2017-08-29 | 2021-06-16 | Bio-Rad Laboratories, Inc. | SYSTEM AND METHOD FOR ISOLATING AND ANALYZING CELLS |
AU2018323875A1 (en) | 2017-09-01 | 2020-04-23 | Gpb Scientific, Inc. | Methods for preparing therapeutically active cells using microfluidics |
US20190226953A1 (en) | 2018-01-19 | 2019-07-25 | International Business Machines Corporation | Microscale and mesoscale condenser devices |
US11458474B2 (en) | 2018-01-19 | 2022-10-04 | International Business Machines Corporation | Microfluidic chips with one or more vias |
US10946380B2 (en) | 2018-01-19 | 2021-03-16 | International Business Machines Corporation | Microfluidic chips for particle purification and fractionation |
WO2019153032A1 (en) * | 2018-02-07 | 2019-08-15 | University Of South Australia | Pre-natal cell isolation |
US11229912B2 (en) | 2018-03-27 | 2022-01-25 | Hewlett-Packard Development Company, L.P. | Particle separation |
WO2019190490A1 (en) * | 2018-03-27 | 2019-10-03 | Hewlett-Packard Development Company, L.P. | Nucleic acid separation |
JP7048940B2 (ja) * | 2018-03-30 | 2022-04-06 | 株式会社日立製作所 | 細胞製造装置 |
US11185861B2 (en) | 2018-06-13 | 2021-11-30 | International Business Machines Corporation | Multistage deterministic lateral displacement device for particle separation |
DE102018210665A1 (de) * | 2018-06-29 | 2020-01-02 | Robert Bosch Gmbh | Mikrofluidische Flusszelle und Verfahren zur Separierung von Zellen |
US10697719B2 (en) * | 2018-08-09 | 2020-06-30 | International Business Machines Corporation | Monitoring a recirculating cooling system for bacterial growth |
US10898895B2 (en) * | 2018-09-13 | 2021-01-26 | Talis Biomedical Corporation | Vented converging capillary biological sample port and reservoir |
AU2019351130A1 (en) | 2018-09-27 | 2021-04-08 | Grail, Llc | Methylation markers and targeted methylation probe panel |
US10967375B2 (en) | 2018-10-23 | 2021-04-06 | International Business Machines Corporation | Microfluidic devices with multiple inlets and outlets |
US11440002B2 (en) * | 2018-10-23 | 2022-09-13 | International Business Machines Corporation | Microfluidic chips with one or more vias filled with sacrificial plugs |
WO2020097048A1 (en) * | 2018-11-05 | 2020-05-14 | Micromedicine, Inc. | Systems and methods for sorting particles using hydrodynamic sizing |
US10633693B1 (en) | 2019-04-16 | 2020-04-28 | Celsee Diagnostics, Inc. | System and method for leakage control in a particle capture system |
US11273439B2 (en) | 2019-05-07 | 2022-03-15 | Bio-Rad Laboratories, Inc. | System and method for target material retrieval from microwells |
CN114072490A (zh) | 2019-05-07 | 2022-02-18 | 伯乐实验室有限公司 | 用于自动化的单细胞加工的系统和方法 |
WO2020251802A1 (en) | 2019-06-14 | 2020-12-17 | Bio-Rad Laboratories, Inc. | System and method for automated single cell processing and analyses |
WO2021011907A1 (en) | 2019-07-18 | 2021-01-21 | Gpb Scientific, Inc. | Ordered processing of blood products to produce therapeutically active cells |
AU2020411478A1 (en) | 2019-12-28 | 2022-07-14 | Gpb Scientific, Inc. | Microfluidic cartridges for processing particles and cells |
US11211144B2 (en) | 2020-02-18 | 2021-12-28 | Tempus Labs, Inc. | Methods and systems for refining copy number variation in a liquid biopsy assay |
US11211147B2 (en) | 2020-02-18 | 2021-12-28 | Tempus Labs, Inc. | Estimation of circulating tumor fraction using off-target reads of targeted-panel sequencing |
US11475981B2 (en) | 2020-02-18 | 2022-10-18 | Tempus Labs, Inc. | Methods and systems for dynamic variant thresholding in a liquid biopsy assay |
US11504719B2 (en) | 2020-03-12 | 2022-11-22 | Bio-Rad Laboratories, Inc. | System and method for receiving and delivering a fluid for sample processing |
US11738288B2 (en) | 2020-06-29 | 2023-08-29 | Jacques Chammas | Automated system and method to isolate specific cells from blood or bone marrow |
CN111733138B (zh) * | 2020-07-30 | 2021-03-30 | 首都医科大学附属北京友谊医院 | 一种循环肿瘤细胞高通量磁力分选方法 |
FR3117884B1 (fr) | 2020-12-21 | 2024-02-16 | Commissariat Energie Atomique | Système de tri par taille de particules expulsées par centrifugation et procédé de configuration d'un tel système |
CN113214959B (zh) * | 2021-04-06 | 2022-08-26 | 深圳市儿童医院 | 一种用于分离捕获尤文肉瘤循环肿瘤细胞的芯片 |
CN114196521A (zh) * | 2021-12-30 | 2022-03-18 | 中国科学院上海微系统与信息技术研究所 | 一种荧光原位杂交芯片及荧光原位杂交方法 |
Family Cites Families (464)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3924947A (en) | 1973-10-19 | 1975-12-09 | Coulter Electronics | Apparatus for preservation and identification of particles analyzed by flow-through apparatus |
US4009435A (en) | 1973-10-19 | 1977-02-22 | Coulter Electronics, Inc. | Apparatus for preservation and identification of particles analyzed by flow-through apparatus |
US3906929A (en) | 1973-11-23 | 1975-09-23 | Lynn Lawrence Augspurger | Processes for reproduction of cellular bodies |
DE2502621C3 (de) | 1975-01-23 | 1978-09-14 | Kernforschungsanlage Juelich Gmbh, 5170 Juelich | Messung elastischer und dielektrischer Eigenschaften der Membran lebender Zellen |
US4115534A (en) | 1976-08-19 | 1978-09-19 | Minnesota Mining And Manufacturing Company | In vitro diagnostic test |
US4190535A (en) | 1978-02-27 | 1980-02-26 | Corning Glass Works | Means for separating lymphocytes and monocytes from anticoagulated blood |
EP0057907B1 (en) * | 1981-02-05 | 1986-12-30 | Asahi Kasei Kogyo Kabushiki Kaisha | Apparatus for separating blood components |
US4415405A (en) | 1981-08-19 | 1983-11-15 | Yale University | Method for engraving a grid pattern on microscope slides and slips |
US4584268A (en) | 1981-10-13 | 1986-04-22 | Ceriani Roberto Luis | Method and compositions for carcinoma diagnosis |
US4434156A (en) | 1981-10-26 | 1984-02-28 | The Salk Institute For Biological Studies | Monoclonal antibodies specific for the human transferrin receptor glycoprotein |
US5310674A (en) | 1982-05-10 | 1994-05-10 | Bar-Ilan University | Apertured cell carrier |
IL68507A (en) | 1982-05-10 | 1986-01-31 | Univ Bar Ilan | System and methods for cell selection |
US4508625A (en) | 1982-10-18 | 1985-04-02 | Graham Marshall D | Magnetic separation using chelated magnetic ions |
EP0162907B1 (en) | 1983-11-08 | 1992-01-15 | Bar Ilan University | System and methods for cell selection |
US4675286A (en) | 1985-01-28 | 1987-06-23 | Aspen Diagnostics, Inc. | Fetal cell separation and testing |
WO1986006170A1 (en) | 1985-04-10 | 1986-10-23 | Immunicon Corporation | Direct homogeneous assay |
US4990326A (en) * | 1985-05-31 | 1991-02-05 | Summa Medical Corporation | Method for detecting blood platelets |
US5164598A (en) * | 1985-08-05 | 1992-11-17 | Biotrack | Capillary flow device |
US4963498A (en) | 1985-08-05 | 1990-10-16 | Biotrack | Capillary flow device |
US4664796A (en) | 1985-09-16 | 1987-05-12 | Coulter Electronics, Inc. | Flux diverting flow chamber for high gradient magnetic separation of particles from a liquid medium |
US4790640A (en) | 1985-10-11 | 1988-12-13 | Nason Frederic L | Laboratory slide |
US4999283A (en) | 1986-01-10 | 1991-03-12 | University Of Kentucky Research Foundation | Method for x and y spermatozoa separation |
US5447841A (en) | 1986-01-16 | 1995-09-05 | The Regents Of The Univ. Of California | Methods for chromosome-specific staining |
US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
JPS62217973A (ja) | 1986-03-20 | 1987-09-25 | 東レ株式会社 | 液体を分別する装置 |
US4906439A (en) | 1986-03-25 | 1990-03-06 | Pb Diagnostic Systems, Inc. | Biological diagnostic device and method of use |
US4789628A (en) | 1986-06-16 | 1988-12-06 | Vxr, Inc. | Devices for carrying out ligand/anti-ligand assays, methods of using such devices and diagnostic reagents and kits incorporating such devices |
US4814098A (en) | 1986-09-06 | 1989-03-21 | Bellex Corporation | Magnetic material-physiologically active substance conjugate |
US4925788A (en) | 1986-10-24 | 1990-05-15 | Immunicon Corporation | Immunoassay system and procedure based on precipitin-like interaction between immune complex and Clq or other non-immunospecific factor |
JP2662215B2 (ja) | 1986-11-19 | 1997-10-08 | 株式会社日立製作所 | 細胞保持装置 |
US4886761A (en) | 1987-03-26 | 1989-12-12 | Yellowstone Diagnostics Corporation | Polysilicon binding assay support and methods |
US4971904A (en) | 1987-06-26 | 1990-11-20 | E. I. Du Pont De Nemours And Company | Heterogeneous immunoassay |
JP2559760B2 (ja) | 1987-08-31 | 1996-12-04 | 株式会社日立製作所 | 細胞搬送方法 |
US4977078A (en) | 1987-12-22 | 1990-12-11 | Olympus Optical Co., Ltd. | Plate substrate immunoassay device and method for performing a multi-test immunoassay on a specimen |
US5039426A (en) | 1988-05-17 | 1991-08-13 | University Of Utah | Process for continuous particle and polymer separation in split-flow thin cells using flow-dependent lift forces |
US5215926A (en) | 1988-06-03 | 1993-06-01 | Cellpro, Inc. | Procedure for designing efficient affinity cell separation processes |
EP0440749B1 (en) | 1988-08-31 | 1997-05-28 | Aprogenex, Inc. | Manual in situ hybridization assay |
US5183744A (en) | 1988-10-26 | 1993-02-02 | Hitachi, Ltd. | Cell handling method for cell fusion processor |
US5101825A (en) | 1988-10-28 | 1992-04-07 | Blackbox, Inc. | Method for noninvasive intermittent and/or continuous hemoglobin, arterial oxygen content, and hematocrit determination |
ATE141957T1 (de) | 1988-11-15 | 1996-09-15 | Univ Yale | In-situ-unterdrückungs-hybridisierung und verwendungen |
US4984574A (en) | 1988-11-23 | 1991-01-15 | Seth Goldberg | Noninvasive fetal oxygen monitor using NMR |
EP0447424B1 (en) | 1988-12-06 | 1998-01-07 | Flinders Technologies Pty. Ltd. | Isolation of fetal cells from maternal blood to enable prenatal diagnosis |
CA1340565C (en) | 1989-06-29 | 1999-05-25 | Thomas B. Okarma | Device and process for cell capture and recovery |
US5698271A (en) | 1989-08-22 | 1997-12-16 | Immunivest Corporation | Methods for the manufacture of magnetically responsive particles |
US5641628A (en) | 1989-11-13 | 1997-06-24 | Children's Medical Center Corporation | Non-invasive method for isolation and detection of fetal DNA |
EP0791659A3 (en) | 1989-11-13 | 1997-09-17 | Children's Medical Center Corporation | Antigen specific separation of nucleated fetal cells in non-invasive detection of fetal DNA |
WO1991007661A1 (en) | 1989-11-20 | 1991-05-30 | Hill Vincent E | A method of detecting drugs in living and post-mortem skin and a kit therefor |
WO1991008304A1 (en) | 1989-11-24 | 1991-06-13 | Isis Innovation Limited | Prenatal genetic determination |
GB8926781D0 (en) | 1989-11-27 | 1990-01-17 | Nat Res Dev | Identification of micro-organisms |
AU647741B2 (en) | 1989-12-01 | 1994-03-31 | Regents Of The University Of California, The | Methods and compositions for chromosome-specific staining |
GB8929057D0 (en) | 1989-12-22 | 1990-02-28 | Gen Electric Co Plc | Sensor |
FR2657543B1 (fr) | 1990-01-26 | 1992-12-18 | Biocom Sa | Dispositif modulaire pour le recueil, l'incubation, la filtration d'echantillons multiples. |
US5327777A (en) | 1990-02-24 | 1994-07-12 | University Of Hertfordshire | Biorheological measurement |
JPH03247276A (ja) | 1990-02-27 | 1991-11-05 | Hitachi Ltd | 細胞の配列方法及び装置 |
US5770029A (en) | 1996-07-30 | 1998-06-23 | Soane Biosciences | Integrated electrophoretic microdevices |
US6054034A (en) | 1990-02-28 | 2000-04-25 | Aclara Biosciences, Inc. | Acrylic microchannels and their use in electrophoretic applications |
US6176962B1 (en) | 1990-02-28 | 2001-01-23 | Aclara Biosciences, Inc. | Methods for fabricating enclosed microchannel structures |
US5858188A (en) | 1990-02-28 | 1999-01-12 | Aclara Biosciences, Inc. | Acrylic microchannels and their use in electrophoretic applications |
US5750015A (en) | 1990-02-28 | 1998-05-12 | Soane Biosciences | Method and device for moving molecules by the application of a plurality of electrical fields |
FR2659347B1 (fr) | 1990-03-12 | 1994-09-02 | Agronomique Inst Nat Rech | Dispositif de culture de cellules assurant leur immobilisation. |
US5153117A (en) | 1990-03-27 | 1992-10-06 | Genetype A.G. | Fetal cell recovery method |
CA2081203A1 (en) | 1990-04-23 | 1991-10-24 | Ronald J. Berenson | Method for enriching fetal cells from maternal blood |
US5147606A (en) | 1990-08-06 | 1992-09-15 | Miles Inc. | Self-metering fluid analysis device |
US6277569B1 (en) | 1990-09-20 | 2001-08-21 | Vysis, Inc. | Methods for multiple direct label probe detection of multiple chromosomes or regions thereof by in situ hybridization |
ATE148503T1 (de) | 1990-09-20 | 1997-02-15 | Amoco Corp | Sonde zusammensetzungen für chromosom identifizierung und verfahren |
US5541072A (en) | 1994-04-18 | 1996-07-30 | Immunivest Corporation | Method for magnetic separation featuring magnetic particles in a multi-phase system |
US5622831A (en) | 1990-09-26 | 1997-04-22 | Immunivest Corporation | Methods and devices for manipulation of magnetically collected material |
US5135627A (en) | 1990-10-15 | 1992-08-04 | Soane Technologies, Inc. | Mosaic microcolumns, slabs, and separation media for electrophoresis and chromatography |
US5217627A (en) | 1990-11-06 | 1993-06-08 | Pall Corporation | System and method for processing biological fluid |
US5646001A (en) | 1991-03-25 | 1997-07-08 | Immunivest Corporation | Affinity-binding separation and release of one or more selected subset of biological entities from a mixed population thereof |
US5186827A (en) | 1991-03-25 | 1993-02-16 | Immunicon Corporation | Apparatus for magnetic separation featuring external magnetic means |
US5466574A (en) | 1991-03-25 | 1995-11-14 | Immunivest Corporation | Apparatus and methods for magnetic separation featuring external magnetic means |
US5173158A (en) | 1991-07-22 | 1992-12-22 | Schmukler Robert E | Apparatus and methods for electroporation and electrofusion |
US5672481A (en) | 1991-10-23 | 1997-09-30 | Cellpro, Incorporated | Apparatus and method for particle separation in a closed field |
US5240856A (en) | 1991-10-23 | 1993-08-31 | Cellpro Incorporated | Apparatus for cell separation |
US5846708A (en) | 1991-11-19 | 1998-12-08 | Massachusetts Institiute Of Technology | Optical and electrical methods and apparatus for molecule detection |
AU4292893A (en) | 1992-04-21 | 1993-11-18 | Regents Of The University Of California, The | Multicolor in situ hybridization methods for genetic testing |
US5304487A (en) | 1992-05-01 | 1994-04-19 | Trustees Of The University Of Pennsylvania | Fluid handling in mesoscale analytical devices |
US5637469A (en) | 1992-05-01 | 1997-06-10 | Trustees Of The University Of Pennsylvania | Methods and apparatus for the detection of an analyte utilizing mesoscale flow systems |
US5726026A (en) | 1992-05-01 | 1998-03-10 | Trustees Of The University Of Pennsylvania | Mesoscale sample preparation device and systems for determination and processing of analytes |
US5498392A (en) | 1992-05-01 | 1996-03-12 | Trustees Of The University Of Pennsylvania | Mesoscale polynucleotide amplification device and method |
ATE174813T1 (de) | 1992-05-01 | 1999-01-15 | Univ Pennsylvania | Polynukleotide amplifikationsanalyse mit einer mikrofabrizierten vorrichtung |
US5486335A (en) | 1992-05-01 | 1996-01-23 | Trustees Of The University Of Pennsylvania | Analysis based on flow restriction |
US5296375A (en) | 1992-05-01 | 1994-03-22 | Trustees Of The University Of Pennsylvania | Mesoscale sperm handling devices |
US6156270A (en) | 1992-05-21 | 2000-12-05 | Biosite Diagnostics, Inc. | Diagnostic devices and apparatus for the controlled movement of reagents without membranes |
US6143576A (en) | 1992-05-21 | 2000-11-07 | Biosite Diagnostics, Inc. | Non-porous diagnostic devices for the controlled movement of reagents |
US5629147A (en) | 1992-07-17 | 1997-05-13 | Aprogenex, Inc. | Enriching and identifying fetal cells in maternal blood for in situ hybridization |
WO1994007138A1 (en) | 1992-09-14 | 1994-03-31 | Fodstad Oystein | Detection of specific target cells in specialized or mixed cell population and solutions containing mixed cell populations |
US5275933A (en) | 1992-09-25 | 1994-01-04 | The Board Of Trustees Of The Leland Stanford Junior University | Triple gradient process for recovering nucleated fetal cells from maternal blood |
US5489506A (en) | 1992-10-26 | 1996-02-06 | Biolife Systems, Inc. | Dielectrophoretic cell stream sorter |
US6953668B1 (en) | 1992-11-05 | 2005-10-11 | Sloan-Kettering Institute For Cancer Research | Prostate-specific membrane antigen |
US5457024A (en) | 1993-01-22 | 1995-10-10 | Aprogenex, Inc. | Isolation of fetal erythrocytes |
US5427663A (en) | 1993-06-08 | 1995-06-27 | British Technology Group Usa Inc. | Microlithographic array for macromolecule and cell fractionation |
US5714325A (en) | 1993-09-24 | 1998-02-03 | New England Medical Center Hospitals | Prenatal diagnosis by isolation of fetal granulocytes from maternal blood |
US5472842A (en) | 1993-10-06 | 1995-12-05 | The Regents Of The University Of California | Detection of amplified or deleted chromosomal regions |
ES2325393T3 (es) | 1993-10-28 | 2009-09-03 | Houston Advanced Research Center | Aparato poroso de flujo a traves microfabricado para la deteccion diferenciada de reacciones de union. |
US6331274B1 (en) | 1993-11-01 | 2001-12-18 | Nanogen, Inc. | Advanced active circuits and devices for molecular biological analysis and diagnostics |
US6068818A (en) | 1993-11-01 | 2000-05-30 | Nanogen, Inc. | Multicomponent devices for molecular biological analysis and diagnostics |
US6315953B1 (en) | 1993-11-01 | 2001-11-13 | Nanogen, Inc. | Devices for molecular biological analysis and diagnostics including waveguides |
NL9401260A (nl) | 1993-11-12 | 1995-06-01 | Cornelis Johannes Maria Van Ri | Membraan voor microfiltratie, ultrafiltratie, gasscheiding en katalyse, werkwijze ter vervaardiging van een dergelijk membraan, mal ter vervaardiging van een dergelijk membraan, alsmede diverse scheidingssystemen omvattende een dergelijk membraan. |
US5432054A (en) | 1994-01-31 | 1995-07-11 | Applied Imaging | Method for separating rare cells from a population of cells |
NO180658C (no) | 1994-03-10 | 1997-05-21 | Oeystein Fodstad | Fremgangsmåte og anordning for deteksjon av spesifikke målceller i spesialiserte eller blandede cellepopulasjoner og opplösninger som inneholder blandede cellepopulasjoner |
US5563067A (en) | 1994-06-13 | 1996-10-08 | Matsushita Electric Industrial Co., Ltd. | Cell potential measurement apparatus having a plurality of microelectrodes |
US6071394A (en) | 1996-09-06 | 2000-06-06 | Nanogen, Inc. | Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis |
US5637458A (en) | 1994-07-20 | 1997-06-10 | Sios, Inc. | Apparatus and method for the detection and assay of organic molecules |
US5648222A (en) | 1994-07-27 | 1997-07-15 | The Trustees Of Columbia University In The City Of New York | Method for preserving cells, and uses of said method |
US6001229A (en) | 1994-08-01 | 1999-12-14 | Lockheed Martin Energy Systems, Inc. | Apparatus and method for performing microfluidic manipulations for chemical analysis |
US5840502A (en) * | 1994-08-31 | 1998-11-24 | Activated Cell Therapy, Inc. | Methods for enriching specific cell-types by density gradient centrifugation |
US5707799A (en) | 1994-09-30 | 1998-01-13 | Abbott Laboratories | Devices and methods utilizing arrays of structures for analyte capture |
US5662813A (en) | 1994-10-21 | 1997-09-02 | Bioseparations, Inc. | Method for separation of nucleated fetal erythrocytes from maternal blood samples |
CA2181190C (en) | 1994-11-14 | 2001-02-06 | Peter Wilding | Mesoscale sample preparation device and systems for determination and processing of analytes |
US5750339A (en) | 1994-11-30 | 1998-05-12 | Thomas Jefferson University | Methods for identifying fetal cells |
US5648220A (en) | 1995-02-14 | 1997-07-15 | New England Medical Center Hospitals, Inc. | Methods for labeling intracytoplasmic molecules |
US6207369B1 (en) | 1995-03-10 | 2001-03-27 | Meso Scale Technologies, Llc | Multi-array, multi-specific electrochemiluminescence testing |
FR2733055B1 (fr) | 1995-04-12 | 1997-12-19 | Chemodyne Sa | Nouveau dispositif d'etude de cultures organotypiques et ses applications en electrophysiologie |
US5709943A (en) | 1995-05-04 | 1998-01-20 | Minnesota Mining And Manufacturing Company | Biological adsorption supports |
DE19520298A1 (de) * | 1995-06-02 | 1996-12-05 | Bayer Ag | Sortiervorrichtung für biologische Zellen oder Viren |
US5715946A (en) * | 1995-06-07 | 1998-02-10 | Reichenbach; Steven H. | Method and apparatus for sorting particles suspended in a fluid |
US5639669A (en) | 1995-06-07 | 1997-06-17 | Ledley; Robert | Separation of fetal cells from maternal blood |
DE69619400T2 (de) | 1995-06-16 | 2002-09-26 | Univ Washington Seattle | Flacher mikrogefertigter querstromfilter für flüssigkeiten |
US6454945B1 (en) | 1995-06-16 | 2002-09-24 | University Of Washington | Microfabricated devices and methods |
US5856174A (en) | 1995-06-29 | 1999-01-05 | Affymetrix, Inc. | Integrated nucleic acid diagnostic device |
US6130098A (en) | 1995-09-15 | 2000-10-10 | The Regents Of The University Of Michigan | Moving microdroplets |
AU1867397A (en) | 1995-11-16 | 1997-06-05 | Michael W. Dahm | Method of quantifying tumour cells in a body fluid and a suitable test kit |
US5863502A (en) | 1996-01-24 | 1999-01-26 | Sarnoff Corporation | Parallel reaction cassette and associated devices |
US5830679A (en) | 1996-03-01 | 1998-11-03 | New England Medical Center Hospitals, Inc. | Diagnostic blood test to identify infants at risk for sepsis |
US5972721A (en) | 1996-03-14 | 1999-10-26 | The United States Of America As Represented By The Secretary Of The Air Force | Immunomagnetic assay system for clinical diagnosis and other purposes |
US5891651A (en) | 1996-03-29 | 1999-04-06 | Mayo Foundation For Medical Education And Research | Methods of recovering colorectal epithelial cells or fragments thereof from stool |
CA2251186A1 (en) | 1996-04-05 | 1997-10-16 | The Johns Hopkins University | A method of enriching rare cells |
US6399023B1 (en) | 1996-04-16 | 2002-06-04 | Caliper Technologies Corp. | Analytical system and method |
US6958245B2 (en) | 1996-04-25 | 2005-10-25 | Bioarray Solutions Ltd. | Array cytometry |
US6387707B1 (en) | 1996-04-25 | 2002-05-14 | Bioarray Solutions | Array Cytometry |
ES2288760T3 (es) | 1996-04-25 | 2008-01-16 | Bioarray Solutions Ltd. | Ensamblaje electrocinetico controlado por luz de particulas proximas a superficies. |
US5989835A (en) | 1997-02-27 | 1999-11-23 | Cellomics, Inc. | System for cell-based screening |
EP0920627B1 (en) | 1996-06-07 | 2004-05-12 | Immunivest Corporation | Magnetic separation employing external and internal gradients |
US6890426B2 (en) | 1996-06-07 | 2005-05-10 | Immunivest Corporation | Magnetic separation apparatus and methods |
NZ333346A (en) | 1996-06-28 | 2000-03-27 | Caliper Techn Corp | High-throughput screening assay systems in microscale fluidic devices |
IT1294964B1 (it) | 1996-07-12 | 1999-04-23 | Domenico Valerio | Isolamento e cultura di cellule fetali dal sangue periferico materno |
US6074827A (en) * | 1996-07-30 | 2000-06-13 | Aclara Biosciences, Inc. | Microfluidic method for nucleic acid purification and processing |
US6280967B1 (en) | 1996-08-02 | 2001-08-28 | Axiom Biotechnologies, Inc. | Cell flow apparatus and method for real-time of cellular responses |
US6100029A (en) | 1996-08-14 | 2000-08-08 | Exact Laboratories, Inc. | Methods for the detection of chromosomal aberrations |
AU4164597A (en) | 1996-08-26 | 1998-03-19 | Princeton University | Reversibly sealable microstructure sorting devices |
DE69709377T2 (de) | 1996-09-04 | 2002-08-14 | Scandinavian Micro Biodevices | Mikrofliesssystem für die teilchenanalyse und trennung |
US6120666A (en) | 1996-09-26 | 2000-09-19 | Ut-Battelle, Llc | Microfabricated device and method for multiplexed electrokinetic focusing of fluid streams and a transport cytometry method using same |
US5858187A (en) | 1996-09-26 | 1999-01-12 | Lockheed Martin Energy Systems, Inc. | Apparatus and method for performing electrodynamic focusing on a microchip |
US6110343A (en) | 1996-10-04 | 2000-08-29 | Lockheed Martin Energy Research Corporation | Material transport method and apparatus |
US5731156A (en) | 1996-10-21 | 1998-03-24 | Applied Imaging, Inc. | Use of anti-embryonic hemoglobin antibodies to identify fetal cells |
US6008010A (en) | 1996-11-01 | 1999-12-28 | University Of Pittsburgh | Method and apparatus for holding cells |
DE19712309A1 (de) | 1996-11-16 | 1998-05-20 | Nmi Univ Tuebingen | Mikroelementenanordnung, Verfahren zum Kontaktieren von in einer flüssigen Umgebung befindlichen Zellen und Verfahren zum Herstellen einer Mikroelementenanordnung |
EP0938674B1 (de) | 1996-11-16 | 2005-06-01 | NMI Naturwissenschaftliches und Medizinisches Institut an der Universität Tübingen in Reutlingen Stiftung Bürgerlichen Rechts | Mikroelementenanordnung, verfahren zum kontaktieren von in einer flüssigen umgebung befindlichen zellen und verfahren zum herstellen einer mikroelementenanordnung |
US6083761A (en) | 1996-12-02 | 2000-07-04 | Glaxo Wellcome Inc. | Method and apparatus for transferring and combining reagents |
DE69723297D1 (de) * | 1996-12-11 | 2003-08-07 | Amersham Plc Little Chalfont | Selektives verfahren zur lyse von zellen |
WO1998028623A1 (en) | 1996-12-20 | 1998-07-02 | Gamera Bioscience Corporation | An affinity binding-based system for detecting particulates in a fluid |
US6235474B1 (en) | 1996-12-30 | 2001-05-22 | The Johns Hopkins University | Methods and kits for diagnosing and determination of the predisposition for diseases |
US6087134A (en) | 1997-01-14 | 2000-07-11 | Applied Imaging Corporation | Method for analyzing DNA from a rare cell in a cell population |
US5879624A (en) | 1997-01-15 | 1999-03-09 | Boehringer Laboratories, Inc. | Method and apparatus for collecting and processing blood |
US6306584B1 (en) | 1997-01-21 | 2001-10-23 | President And Fellows Of Harvard College | Electronic-property probing of biological molecules at surfaces |
US6008007A (en) | 1997-01-31 | 1999-12-28 | Oncotech, Inc. | Radiation resistance assay for predicting treatment response and clinical outcome |
US6056859A (en) | 1997-02-12 | 2000-05-02 | Lockheed Martin Energy Research Corporation | Method and apparatus for staining immobilized nucleic acids |
AP9901660A0 (en) * | 1997-02-28 | 1999-09-30 | Burstein Lab Inc | Laboratory in a disk. |
GB9704444D0 (en) | 1997-03-04 | 1997-04-23 | Isis Innovation | Non-invasive prenatal diagnosis |
GB9704876D0 (en) | 1997-03-08 | 1997-04-23 | Univ Dundee | Diagnostic methods |
GB2326943B (en) | 1997-03-08 | 1999-06-16 | Univ Dundee | Detection of fetal red cells in maternal blood |
US6623983B1 (en) | 1997-03-25 | 2003-09-23 | Immunivest Corporation | Apparatus and methods for capture and analysis of particulate entities |
US6391622B1 (en) | 1997-04-04 | 2002-05-21 | Caliper Technologies Corp. | Closed-loop biochemical analyzers |
US6066449A (en) | 1997-04-15 | 2000-05-23 | The Trustees Of Columbia University In The City Of New York | Method of detecting metastatic thyroid cancer |
AU727083B2 (en) | 1997-04-25 | 2000-11-30 | Caliper Life Sciences, Inc. | Microfluidic devices incorporating improved channel geometries |
WO1998049344A1 (en) | 1997-04-28 | 1998-11-05 | Lockheed Martin Energy Research Corporation | Method and apparatus for analyzing nucleic acids |
US6169816B1 (en) | 1997-05-14 | 2001-01-02 | Applied Imaging, Inc. | Identification of objects of interest using multiple illumination schemes and finding overlap of features in corresponding multiple images |
US6632619B1 (en) | 1997-05-16 | 2003-10-14 | The Governors Of The University Of Alberta | Microfluidic system and methods of use |
US6156273A (en) | 1997-05-27 | 2000-12-05 | Purdue Research Corporation | Separation columns and methods for manufacturing the improved separation columns |
US5869004A (en) | 1997-06-09 | 1999-02-09 | Caliper Technologies Corp. | Methods and apparatus for in situ concentration and/or dilution of materials in microfluidic systems |
CA2293744A1 (en) | 1997-06-12 | 1998-12-17 | Clinical Micro Sensors, Inc. | Electronic methods for the detection of analytes |
US5882465A (en) | 1997-06-18 | 1999-03-16 | Caliper Technologies Corp. | Method of manufacturing microfluidic devices |
SG115335A1 (en) | 1997-07-04 | 2005-10-28 | Tokyo Electron Ltd | Process solution supplying apparatus |
US5876675A (en) | 1997-08-05 | 1999-03-02 | Caliper Technologies Corp. | Microfluidic devices and systems |
US6368871B1 (en) * | 1997-08-13 | 2002-04-09 | Cepheid | Non-planar microstructures for manipulation of fluid samples |
CA2301309A1 (en) | 1997-08-13 | 1999-02-25 | Cepheid | Microstructures for the manipulation of fluid samples |
US7214298B2 (en) | 1997-09-23 | 2007-05-08 | California Institute Of Technology | Microfabricated cell sorter |
US6540895B1 (en) | 1997-09-23 | 2003-04-01 | California Institute Of Technology | Microfabricated cell sorter for chemical and biological materials |
US5842787A (en) | 1997-10-09 | 1998-12-01 | Caliper Technologies Corporation | Microfluidic systems incorporating varied channel dimensions |
US6241894B1 (en) | 1997-10-10 | 2001-06-05 | Systemix | High gradient magnetic device and method for cell separation or purification |
US5962234A (en) | 1997-10-20 | 1999-10-05 | Applied Imaging Corporation | Use of anti-embryonic epsilon hemoglobin antibodies to identify fetal cells |
US5962250A (en) | 1997-10-28 | 1999-10-05 | Glaxo Group Limited | Split multi-well plate and methods |
US6197523B1 (en) | 1997-11-24 | 2001-03-06 | Robert A. Levine | Method for the detection, identification, enumeration and confirmation of circulating cancer and/or hematologic progenitor cells in whole blood |
AU9233698A (en) | 1997-11-22 | 1999-06-17 | Robert A. Levine | Method for the detection, identification, enumeration and confirmation of circulating cancer cells and/or hematologic progenitor cells in whole blood |
JP3486171B2 (ja) | 1997-12-17 | 2004-01-13 | エコル・ポリテクニック・フェデラル・ドゥ・ロザンヌ(エ・ペー・エフ・エル) | ミクロ構造キャリア上における細胞単体および再構成膜系のポジショニングおよび電気生理学的特性決定 |
US6210889B1 (en) | 1998-01-28 | 2001-04-03 | The Universite Laval | Method for enrichment of fetal cells from maternal blood and use of same in determination of fetal sex and detection of chromosomal abnormalities |
US20020172987A1 (en) | 1998-02-12 | 2002-11-21 | Terstappen Leon W.M.M. | Methods and reagents for the rapid and efficient isolation of circulating cancer cells |
AU760560B2 (en) | 1998-02-12 | 2003-05-15 | Board Of Regents, The University Of Texas System | Methods and reagents for the rapid and efficient isolation of circulating cancer cells |
US20010018192A1 (en) | 1998-02-12 | 2001-08-30 | Terstappen Leon W.M.M. | Labeled cells for use as an internal functional control in rare cell detection assays |
US6537505B1 (en) | 1998-02-20 | 2003-03-25 | Bio Dot, Inc. | Reagent dispensing valve |
US6036857A (en) | 1998-02-20 | 2000-03-14 | Florida State University Research Foundation, Inc. | Apparatus for continuous magnetic separation of components from a mixture |
US6251343B1 (en) | 1998-02-24 | 2001-06-26 | Caliper Technologies Corp. | Microfluidic devices and systems incorporating cover layers |
WO1999044064A1 (en) | 1998-02-27 | 1999-09-02 | Cli Oncology, Inc. | Method and compositions for differential detection of primary tumor cells and metastatic cells |
US6210910B1 (en) | 1998-03-02 | 2001-04-03 | Trustees Of Tufts College | Optical fiber biosensor array comprising cell populations confined to microcavities |
US6027623A (en) * | 1998-04-22 | 2000-02-22 | Toyo Technologies, Inc. | Device and method for electrophoretic fraction |
US6100033A (en) | 1998-04-30 | 2000-08-08 | The Regents Of The University Of California | Diagnostic test for prenatal identification of Down's syndrome and mental retardation and gene therapy therefor |
US6200765B1 (en) | 1998-05-04 | 2001-03-13 | Pacific Northwest Cancer Foundation | Non-invasive methods to detect prostate cancer |
EP1190229B1 (en) | 1998-05-22 | 2011-10-26 | California Institute Of Technology | Microfabricated cell sorter |
US6306589B1 (en) | 1998-05-27 | 2001-10-23 | Vysis, Inc. | Biological assays for analyte detection |
WO1999063332A1 (en) | 1998-06-05 | 1999-12-09 | Sarnoff Corporation | Apparatus for separating molecules |
US6529835B1 (en) | 1998-06-25 | 2003-03-04 | Caliper Technologies Corp. | High throughput methods, systems and apparatus for performing cell based screening assays |
US7339033B2 (en) | 1998-06-26 | 2008-03-04 | Genentech, Inc. | Pro1481 |
US6465225B1 (en) | 1998-06-29 | 2002-10-15 | Evotec Oai Ag | Method and device for manipulating particles in microsystems |
US6045990A (en) | 1998-07-09 | 2000-04-04 | Baust; John M. | Inclusion of apoptotic regulators in solutions for cell storage at low temperature |
US6576478B1 (en) | 1998-07-14 | 2003-06-10 | Zyomyx, Inc. | Microdevices for high-throughput screening of biomolecules |
US6897073B2 (en) | 1998-07-14 | 2005-05-24 | Zyomyx, Inc. | Non-specific binding resistant protein arrays and methods for making the same |
US6274339B1 (en) | 1999-02-05 | 2001-08-14 | Millennium Pharmaceuticals, Inc. | Methods and compositions for the diagnosis and treatment of body weight disorders, including obesity |
FR2782730B1 (fr) | 1998-08-25 | 2002-05-17 | Biocom Sa | Procede de separation cellulaire pour l'isolation de cellules pathogeniques, notamment cancereuses rares, equipement et reactif pour la mise en oeuvre du procede et application du procede |
US6245227B1 (en) | 1998-09-17 | 2001-06-12 | Kionix, Inc. | Integrated monolithic microfabricated electrospray and liquid chromatography system and method |
ES2172353T3 (es) | 1998-09-18 | 2002-09-16 | Micromet Ag | Amplificacion del adn de una unica celula. |
US6656697B1 (en) * | 1998-09-28 | 2003-12-02 | Lifescan, Inc. | Diagnostics based on tetrazolium compounds |
US6637463B1 (en) | 1998-10-13 | 2003-10-28 | Biomicro Systems, Inc. | Multi-channel microfluidic system design with balanced fluid flow distribution |
US6086740A (en) | 1998-10-29 | 2000-07-11 | Caliper Technologies Corp. | Multiplexed microfluidic devices and systems |
US6277489B1 (en) | 1998-12-04 | 2001-08-21 | The Regents Of The University Of California | Support for high performance affinity chromatography and other uses |
US6062261A (en) | 1998-12-16 | 2000-05-16 | Lockheed Martin Energy Research Corporation | MicrofluIdic circuit designs for performing electrokinetic manipulations that reduce the number of voltage sources and fluid reservoirs |
BR9916840A (pt) | 1998-12-23 | 2001-10-09 | Nanogen Inc | Sistema de detecção biológica portátil integrado |
US6636498B1 (en) * | 1999-01-08 | 2003-10-21 | Cisco Technology, Inc. | Mobile IP mobile router |
US6150119A (en) | 1999-01-19 | 2000-11-21 | Caliper Technologies Corp. | Optimized high-throughput analytical system |
US6960449B2 (en) | 1999-02-10 | 2005-11-01 | Cell Works Diagnostics, Inc. | Class characterization of circulating cancer cells isolated from body fluids and methods of use |
EP1163052B1 (en) * | 1999-02-23 | 2010-06-02 | Caliper Life Sciences, Inc. | Manipulation of microparticles in microfluidic systems |
WO2000052446A1 (en) | 1999-03-02 | 2000-09-08 | Qualigen, Inc. | Methods and apparatus for separation of biological fluids |
US6942978B1 (en) | 1999-03-03 | 2005-09-13 | The Board Of Trustees Of The University Of Arkansas | Transmembrane serine protease overexpressed in ovarian carcinoma and uses thereof |
CN1185492C (zh) | 1999-03-15 | 2005-01-19 | 清华大学 | 可单点选通式微电磁单元阵列芯片、电磁生物芯片及应用 |
TW496775B (en) | 1999-03-15 | 2002-08-01 | Aviva Bioscience Corp | Individually addressable micro-electromagnetic unit array chips |
US6858439B1 (en) | 1999-03-15 | 2005-02-22 | Aviva Biosciences | Compositions and methods for separation of moieties on chips |
NZ513390A (en) | 1999-03-19 | 2003-01-31 | Genencor Int | Multi-through hole testing plate for high throughput screening |
US6368562B1 (en) | 1999-04-16 | 2002-04-09 | Orchid Biosciences, Inc. | Liquid transportation system for microfluidic device |
US6942771B1 (en) | 1999-04-21 | 2005-09-13 | Clinical Micro Sensors, Inc. | Microfluidic systems in the electrochemical detection of target analytes |
US6511967B1 (en) * | 1999-04-23 | 2003-01-28 | The General Hospital Corporation | Use of an internalizing transferrin receptor to image transgene expression |
US6174683B1 (en) | 1999-04-26 | 2001-01-16 | Biocept, Inc. | Method of making biochips and the biochips resulting therefrom |
US6589791B1 (en) | 1999-05-20 | 2003-07-08 | Cartesian Technologies, Inc. | State-variable control system |
DE10084613T1 (de) | 1999-05-21 | 2002-09-26 | Univ Leland Stanford Junior | Mikrofluidvorrichtung und Verfahren zum Erzeugen pulsierender Mikrofluidstrahlen in einer Flüssigumgebung |
US6635163B1 (en) | 1999-06-01 | 2003-10-21 | Cornell Research Foundation, Inc. | Entropic trapping and sieving of molecules |
US6878540B2 (en) | 1999-06-25 | 2005-04-12 | Cepheid | Device for lysing cells, spores, or microorganisms |
US6818395B1 (en) | 1999-06-28 | 2004-11-16 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences |
US6395232B1 (en) | 1999-07-09 | 2002-05-28 | Orchid Biosciences, Inc. | Fluid delivery system for a microfluidic device using a pressure pulse |
US6524456B1 (en) | 1999-08-12 | 2003-02-25 | Ut-Battelle, Llc | Microfluidic devices for the controlled manipulation of small volumes |
US6762059B2 (en) | 1999-08-13 | 2004-07-13 | U.S. Genomics, Inc. | Methods and apparatuses for characterization of single polymers |
US6613581B1 (en) * | 1999-08-26 | 2003-09-02 | Caliper Technologies Corp. | Microfluidic analytic detection assays, devices, and integrated systems |
US6623945B1 (en) | 1999-09-16 | 2003-09-23 | Motorola, Inc. | System and method for microwave cell lysing of small samples |
FR2798673B1 (fr) | 1999-09-16 | 2004-05-28 | Exonhit Therapeutics Sa | Methodes et compositions pour la detection d'evenements pathologiques |
US20030113528A1 (en) | 1999-09-17 | 2003-06-19 | Wilson Moya | Patterned porous structures |
EP1218547A4 (en) | 1999-10-15 | 2005-04-20 | Ventana Med Syst Inc | METHOD FOR DETECTING UNIQUE GENE IN SITU COPIES |
AU4504001A (en) | 1999-11-04 | 2001-06-04 | Princeton University | Electrodeless dielectrophoresis for polarizable particles |
US6692952B1 (en) | 1999-11-10 | 2004-02-17 | Massachusetts Institute Of Technology | Cell analysis and sorting apparatus for manipulation of cells |
US20060128006A1 (en) | 1999-11-10 | 2006-06-15 | Gerhardt Antimony L | Hydrodynamic capture and release mechanisms for particle manipulation |
AU1592501A (en) | 1999-11-10 | 2001-06-06 | Massachusetts Institute Of Technology | Cell analysis and sorting apparatus for manipulation of cells |
US6875619B2 (en) | 1999-11-12 | 2005-04-05 | Motorola, Inc. | Microfluidic devices comprising biochannels |
US6361958B1 (en) | 1999-11-12 | 2002-03-26 | Motorola, Inc. | Biochannel assay for hybridization with biomaterial |
US6605453B2 (en) | 1999-12-01 | 2003-08-12 | The Regents Of The University Of California | Electric-field-assisted fluidic assembly of inorganic and organic materials, molecules and like small things including living cells |
US6309889B1 (en) | 1999-12-23 | 2001-10-30 | Glaxo Wellcome Inc. | Nano-grid micro reactor and methods |
US6379884B2 (en) | 2000-01-06 | 2002-04-30 | Caliper Technologies Corp. | Methods and systems for monitoring intracellular binding reactions |
CA2397341A1 (en) | 2000-01-13 | 2001-07-19 | Dhanesh Gohel | Ferrofluid based arrays |
WO2001071026A2 (de) | 2000-03-20 | 2001-09-27 | Adnagen Ag | Kit, verfahren und mikroarray zur bestimmung des geschlechtes eines menschlichen fötus |
ES2548381T3 (es) | 2000-03-27 | 2015-10-16 | Thomas Jefferson University | Composiciones para el tratamiento e imágenes de células de cáncer estomacales y de esófago |
US20030186889A1 (en) | 2000-03-31 | 2003-10-02 | Wolf-Georg Forssmann | Diagnostic and medicament for analysing the cell surface proteome of tumour and inflammatory cells and for treating tumorous and inflammatory diseases, preferably using a specific chemokine receptor analysis and the chemokine receptor-ligand interaction |
CA2404978A1 (en) | 2000-04-03 | 2001-10-11 | Corixa Corporation | Methods, compositions and kits for the detection and monitoring of breast cancer |
US20030170631A1 (en) | 2000-04-03 | 2003-09-11 | Corixa Corporation | Methods, compositions and kits for the detection and monitoring of breast cancer |
AU2001252973A1 (en) | 2000-04-17 | 2001-10-30 | Purdue Research Foundation | Biosensor and related method |
DE10019877A1 (de) | 2000-04-20 | 2001-10-25 | Clariant Gmbh | Wasch- und Reinigungsmittel enthaltend bleichaktive Dendrimer-Liganden und deren Metall-Komplexe |
GB0009784D0 (en) | 2000-04-20 | 2000-06-07 | Simeg Limited | Methods for clinical diagnosis |
WO2001081621A2 (de) | 2000-04-20 | 2001-11-01 | Adnagen Ag | Verfahren, diagnose-kit und mikroarray zur bestimmung des rhesus-faktors |
US7641856B2 (en) | 2004-05-14 | 2010-01-05 | Honeywell International Inc. | Portable sample analyzer with removable cartridge |
US6790330B2 (en) | 2000-06-14 | 2004-09-14 | Board Of Regents, The University Of Texas System | Systems and methods for cell subpopulation analysis |
DE60117556T2 (de) | 2000-06-21 | 2006-11-02 | Bioarray Solutions Ltd. | Multianalytische molekularanalyse durch verwendung anwendungsspezifischer zufallspartikelarrays |
US20020012616A1 (en) * | 2000-07-03 | 2002-01-31 | Xiaochuan Zhou | Fluidic methods and devices for parallel chemical reactions |
KR100845638B1 (ko) | 2000-07-17 | 2008-07-10 | 엡슨 토요콤 가부시키가이샤 | 압전발진기 |
DE10035433C2 (de) | 2000-07-20 | 2002-07-18 | Tuma Wolfgang | Schonende Hochanreicherung von fetalen Zellen aus pripherem Blut und Verwendung derselben |
AU2000274922A1 (en) * | 2000-08-08 | 2002-02-18 | Aviva Biosciences Corporation | Methods for manipulating moieties in microfluidic systems |
US20040005582A1 (en) * | 2000-08-10 | 2004-01-08 | Nanobiodynamics, Incorporated | Biospecific desorption microflow systems and methods for studying biospecific interactions and their modulators |
US6610499B1 (en) | 2000-08-31 | 2003-08-26 | The Regents Of The University Of California | Capillary array and related methods |
US6818424B2 (en) | 2000-09-01 | 2004-11-16 | E. I. Du Pont De Nemours And Company | Production of cyclic terpenoids |
US20020164825A1 (en) | 2000-09-09 | 2002-11-07 | Wen-Tien Chen | Cell separation matrix |
AU2001288437C1 (en) | 2000-09-09 | 2009-05-21 | The Research Foundation Of State University Of New York | Method and compositions for isolating metastatic cancer cells, and use in measuring metastatic potential of a cancer thereof |
EP1334347A1 (en) | 2000-09-15 | 2003-08-13 | California Institute Of Technology | Microfabricated crossflow devices and methods |
CA2421828A1 (en) | 2000-09-30 | 2002-04-11 | Xiao-Bo Wang | Apparatuses containing multiple force generating elements and uses thereof |
US6689615B1 (en) | 2000-10-04 | 2004-02-10 | James Murto | Methods and devices for processing blood samples |
WO2002031506A1 (en) | 2000-10-09 | 2002-04-18 | Aviva Biosciences Coropration | Compositions and methods for separation of moieties on chips |
EP1328342A4 (en) | 2000-10-10 | 2006-03-15 | Aviva Biosciences Corp | INTEGRATED BIOCHIP SYSTEM FOR SAMPLE PREPARATION AND ANALYSIS |
US20050100951A1 (en) | 2000-10-26 | 2005-05-12 | Biocept, Inc. | 3D format biochips and method of use |
US6784420B2 (en) | 2000-11-13 | 2004-08-31 | Genoptix, Inc. | Method of separating particles using an optical gradient |
US6833542B2 (en) | 2000-11-13 | 2004-12-21 | Genoptix, Inc. | Method for sorting particles |
US20020108859A1 (en) | 2000-11-13 | 2002-08-15 | Genoptix | Methods for modifying interaction between dielectric particles and surfaces |
US6744038B2 (en) | 2000-11-13 | 2004-06-01 | Genoptix, Inc. | Methods of separating particles using an optical gradient |
US20020123112A1 (en) | 2000-11-13 | 2002-09-05 | Genoptix | Methods for increasing detection sensitivity in optical dielectric sorting systems |
US20030007894A1 (en) | 2001-04-27 | 2003-01-09 | Genoptix | Methods and apparatus for use of optical forces for identification, characterization and/or sorting of particles |
US20020115163A1 (en) | 2000-11-13 | 2002-08-22 | Genoptix | Methods for sorting particles by size and elasticity |
AU2002243263A1 (en) | 2000-11-15 | 2002-07-24 | Roche Diagnostics Corporation | Methods and reagents for identifying rare fetal cells in the material circulation |
US6521188B1 (en) | 2000-11-22 | 2003-02-18 | Industrial Technology Research Institute | Microfluidic actuator |
US6778724B2 (en) | 2000-11-28 | 2004-08-17 | The Regents Of The University Of California | Optical switching and sorting of biological samples and microparticles transported in a micro-fluidic device, including integrated bio-chip devices |
US6495340B2 (en) | 2000-11-28 | 2002-12-17 | Medis El Ltd. | Cell carrier grids |
WO2002044689A2 (en) | 2000-11-28 | 2002-06-06 | The Regents Of The University Of California | Storing microparticles in optical switch which is transported by micro-fluidic device |
US20020064808A1 (en) | 2000-11-29 | 2002-05-30 | Mutz Mitchell W. | Focused acoustic energy for ejecting cells from a fluid |
US6849423B2 (en) | 2000-11-29 | 2005-02-01 | Picoliter Inc | Focused acoustics for detection and sorting of fluid volumes |
US6893836B2 (en) | 2000-11-29 | 2005-05-17 | Picoliter Inc. | Spatially directed ejection of cells from a carrier fluid |
AU2002219979A1 (en) * | 2000-12-01 | 2002-06-11 | Burstein Technologies, Inc. | Apparatus and methods for separating components of particulate suspension |
FR2817967B1 (fr) | 2000-12-08 | 2003-02-28 | Diagast | Procede de magnetisation de marqueurs chimiques ou biologiques |
US6770434B2 (en) | 2000-12-29 | 2004-08-03 | The Provost, Fellows And Scholars Of The College Of The Holy & Undivided Trinity Of Queen Elizabeth Near Dublin | Biological assay method |
US7205157B2 (en) | 2001-01-08 | 2007-04-17 | Becton, Dickinson And Company | Method of separating cells from a sample |
US6453928B1 (en) | 2001-01-08 | 2002-09-24 | Nanolab Ltd. | Apparatus, and method for propelling fluids |
US20020160363A1 (en) | 2001-01-31 | 2002-10-31 | Mcdevitt John T. | Magnetic-based placement and retention of sensor elements in a sensor array |
US20020106715A1 (en) | 2001-02-02 | 2002-08-08 | Medisel Ltd | System and method for collecting data from individual cells |
US20020110835A1 (en) | 2001-02-13 | 2002-08-15 | Rajan Kumar | Microfluidic devices and methods |
US6913697B2 (en) | 2001-02-14 | 2005-07-05 | Science & Technology Corporation @ Unm | Nanostructured separation and analysis devices for biological membranes |
US6685841B2 (en) | 2001-02-14 | 2004-02-03 | Gabriel P. Lopez | Nanostructured devices for separation and analysis |
US20030190602A1 (en) | 2001-03-12 | 2003-10-09 | Monogen, Inc. | Cell-based detection and differentiation of disease states |
US20030199685A1 (en) | 2001-03-12 | 2003-10-23 | Monogen, Inc. | Cell-based detection and differentiation of disease states |
US7323140B2 (en) | 2001-03-28 | 2008-01-29 | Handylab, Inc. | Moving microdroplets in a microfluidic device |
US20020160518A1 (en) | 2001-04-03 | 2002-10-31 | Hayenga Jon W. | Microfluidic sedimentation |
US20020173043A1 (en) | 2001-04-04 | 2002-11-21 | Eddine Merabet | Cyanide-free reagent, and method for detecting hemoglobin |
US20030036100A1 (en) | 2001-04-10 | 2003-02-20 | Imperial College Innovations Ltd. | Simultaneous determination of phenotype and genotype |
US20030040119A1 (en) * | 2001-04-11 | 2003-02-27 | The Regents Of The University Of Michigan | Separation devices and methods for separating particles |
US7713705B2 (en) | 2002-12-24 | 2010-05-11 | Biosite, Inc. | Markers for differential diagnosis and methods of use thereof |
FR2824144B1 (fr) | 2001-04-30 | 2004-09-17 | Metagenex S A R L | Methode de diagnostic prenatal sur cellule foetale isolee du sang maternel |
AU2002305436A1 (en) | 2001-05-09 | 2002-11-18 | Virginia Commonwealth University | Multiple sequencible and ligatible structures for genomic analysis |
US6805841B2 (en) | 2001-05-09 | 2004-10-19 | The Provost Fellows And Scholars Of The College Of The Holy And Undivided Trinity Of Queen Elizabeth Near Dublin | Liquid pumping system |
US20020166760A1 (en) * | 2001-05-11 | 2002-11-14 | Prentiss Mara G. | Micromagentic systems and methods for microfluidics |
US6743636B2 (en) | 2001-05-24 | 2004-06-01 | Industrial Technology Research Institute | Microfluid driving device |
DE10127079A1 (de) | 2001-06-02 | 2002-12-12 | Ulrich Pachmann | Verfahren zum quantitativen Nachweis vitaler epithelialer Tumorzellen in einer Körperflüssigkeit |
JP2005514187A (ja) | 2001-06-20 | 2005-05-19 | サイトノーム インコーポレーテッド | 流体をマイクロ流体システムと相互接続するための仮想壁流体相互接続ポートを含むマイクロ流体システム |
US7419574B2 (en) | 2001-06-20 | 2008-09-02 | Cummings Eric B | Dielectrophoresis device and method having non-uniform arrays for manipulating particles |
US20060019235A1 (en) | 2001-07-02 | 2006-01-26 | The Board Of Trustees Of The Leland Stanford Junior University | Molecular and functional profiling using a cellular microarray |
EP1413346A4 (en) | 2001-08-03 | 2005-05-25 | Nec Corp | SEPARATING DEVICE AND METHOD FOR PRODUCING THE SEPARATOR |
US6881315B2 (en) | 2001-08-03 | 2005-04-19 | Nec Corporation | Fractionating apparatus having colonies of pillars arranged in migration passage at interval and process for fabricating pillars |
JP2005501236A (ja) | 2001-08-23 | 2005-01-13 | イムニベスト・コーポレイション | 分析用の細胞および生物学的検体の安定化 |
US7863012B2 (en) | 2004-02-17 | 2011-01-04 | Veridex, Llc | Analysis of circulating tumor cells, fragments, and debris |
EP2269736B1 (en) | 2001-08-28 | 2013-04-24 | Gyros Patent Ab | Retaining microfluidic microcavity and other microfluidic structures |
ES2284911T3 (es) | 2001-09-04 | 2007-11-16 | Iq Corporation B.V. | Determinacion y cuantificacion de poblaciones de globulos rojos en muestras. |
DE10143776A1 (de) | 2001-09-06 | 2003-04-03 | Adnagen Ag | Verfahren und Kit zur Diagnostik oder Behandlungskontrolle von Brustkrebs |
EP1409727B1 (de) | 2001-09-06 | 2005-11-09 | Adnagen AG | Verfahren zum qualitativen und/oder quantitativen nachweis von zellen |
US7202045B2 (en) | 2001-09-19 | 2007-04-10 | Regents Of The University Of Michigan | Detection and treatment of cancers of the lung |
US20030072682A1 (en) | 2001-10-11 | 2003-04-17 | Dan Kikinis | Method and apparatus for performing biochemical testing in a microenvironment |
DK1439897T3 (da) | 2001-10-11 | 2011-01-03 | Aviva Biosciences Corp | Fremgangsmåder til adskillelse af sjældne celler fra væskeprøver |
US8980568B2 (en) | 2001-10-11 | 2015-03-17 | Aviva Biosciences Corporation | Methods and compositions for detecting non-hematopoietic cells from a blood sample |
US7166443B2 (en) | 2001-10-11 | 2007-01-23 | Aviva Biosciences Corporation | Methods, compositions, and automated systems for separating rare cells from fluid samples |
US6783647B2 (en) | 2001-10-19 | 2004-08-31 | Ut-Battelle, Llc | Microfluidic systems and methods of transport and lysis of cells and analysis of cell lysate |
AU2002360305A1 (en) | 2001-10-26 | 2003-05-06 | Immunivest Corporation | Multiparameter analysis of comprehensive nucleic acids and morphological features on the same sample |
WO2003037400A2 (en) | 2001-10-31 | 2003-05-08 | Ventrigraft Inc | Methods and device compositions for the recruitment of cells to blood contacting surfaces in vivo |
WO2003040331A2 (en) | 2001-11-07 | 2003-05-15 | Origene Technologies, Inc. | Prostate cancer genes |
US20030232350A1 (en) | 2001-11-13 | 2003-12-18 | Eos Biotechnology, Inc. | Methods of diagnosis of cancer, compositions and methods of screening for modulators of cancer |
US20050244843A1 (en) | 2001-11-16 | 2005-11-03 | Wen-Tien Chen | Blood test prototypes and methods for the detection of circulating tumor and endothelial cells |
WO2003044519A1 (fr) * | 2001-11-20 | 2003-05-30 | Nec Corporation | Appareil de separation, procede de separation et procede de production d'un appareil de separation |
AU2002219137A1 (en) | 2001-11-22 | 2003-06-10 | Adnagen Ag | Diagnosis kit, dna chip, and methods for diagnosing or supervising the treatment of testicular cancer |
CA2469878C (en) | 2001-12-11 | 2011-06-28 | Netech Inc. | Blood cell separating system |
US20040241653A1 (en) | 2001-12-31 | 2004-12-02 | Elena Feinstein | Methods for identifying marker genes for cancer |
EP1466162A1 (en) | 2002-01-10 | 2004-10-13 | Board Of Regents The University Of Texas System | Flow sorting system and methods regarding same |
US7383134B2 (en) | 2002-01-15 | 2008-06-03 | Piper James R | Method and/or system for analyzing biological samples using a computer system |
US7318902B2 (en) | 2002-02-04 | 2008-01-15 | Colorado School Of Mines | Laminar flow-based separations of colloidal and cellular particles |
AU2003219759B2 (en) | 2002-02-14 | 2011-01-20 | Veridex, Llc | Methods and algorithms for cell enumeration in a low-cost cytometer |
US7053967B2 (en) | 2002-05-23 | 2006-05-30 | Planar Systems, Inc. | Light sensitive display |
FR2836072B1 (fr) | 2002-02-21 | 2004-11-12 | Commissariat Energie Atomique | Composant utilisant un materiau composite et destine a un microsysteme d'analyse biologique ou biochimique |
FR2836071B1 (fr) | 2002-02-21 | 2005-02-04 | Commissariat Energie Atomique | Composant pour microsysteme d'analyse biologique ou biochimique |
US6958119B2 (en) | 2002-02-26 | 2005-10-25 | Agilent Technologies, Inc. | Mobile phase gradient generation microfluidic device |
US7223371B2 (en) | 2002-03-14 | 2007-05-29 | Micronics, Inc. | Microfluidic channel network device |
SE0200860D0 (sv) | 2002-03-20 | 2002-03-20 | Monica Almqvist | Microfluidic cell and method for sample handling |
WO2004023105A2 (en) | 2002-03-20 | 2004-03-18 | Advanced Sensor Technologies, Inc. | Personal monitor to detect exposure to toxic agents |
US7312085B2 (en) | 2002-04-01 | 2007-12-25 | Fluidigm Corporation | Microfluidic particle-analysis systems |
AU2003224817B2 (en) | 2002-04-01 | 2008-11-06 | Fluidigm Corporation | Microfluidic particle-analysis systems |
US20040241707A1 (en) | 2002-04-01 | 2004-12-02 | Gao Chun L. | Enhanced diagnostic potential of prostate-specific antigen expressing cells |
US7141369B2 (en) | 2002-04-25 | 2006-11-28 | Semibio Technology, Inc. | Measuring cellular metabolism of immobilized cells |
JP2005524833A (ja) | 2002-05-03 | 2005-08-18 | イムニベスト・コーポレイション | 分析細胞イメージング用のデバイスおよび方法 |
EP1507548B1 (en) | 2002-05-27 | 2014-10-01 | CanImGuide Therapeutics AB | Method for determining immune system affecting compounds |
SE0201738D0 (sv) * | 2002-06-07 | 2002-06-07 | Aamic Ab | Micro-fluid structures |
US20040005247A1 (en) | 2002-07-03 | 2004-01-08 | Nanostream, Inc. | Microfluidic closed-end metering systems and methods |
US20040101444A1 (en) | 2002-07-15 | 2004-05-27 | Xeotron Corporation | Apparatus and method for fluid delivery to a hybridization station |
US20040018611A1 (en) | 2002-07-23 | 2004-01-29 | Ward Michael Dennis | Microfluidic devices for high gradient magnetic separation |
US20040019300A1 (en) * | 2002-07-26 | 2004-01-29 | Leonard Leslie Anne | Microfluidic blood sample separations |
US7214348B2 (en) | 2002-07-26 | 2007-05-08 | Applera Corporation | Microfluidic size-exclusion devices, systems, and methods |
US7118676B2 (en) * | 2003-09-04 | 2006-10-10 | Arryx, Inc. | Multiple laminar flow-based particle and cellular separation with laser steering |
US9435799B2 (en) | 2002-07-31 | 2016-09-06 | Janssen Diagnostics, Inc. | Methods and reagents for improved selection of biological materials |
AU2003259722A1 (en) | 2002-08-08 | 2004-02-25 | Nanostream, Inc. | Systems and methods for high-throughput microfluidic sample analysis |
US20060008807A1 (en) | 2002-08-23 | 2006-01-12 | O'hara Shawn M | Multiparameter analysis of comprehensive nucleic acids and morphological features on the same sample |
US20040043506A1 (en) | 2002-08-30 | 2004-03-04 | Horst Haussecker | Cascaded hydrodynamic focusing in microfluidic channels |
US7094345B2 (en) | 2002-09-09 | 2006-08-22 | Cytonome, Inc. | Implementation of microfluidic components, including molecular fractionation devices, in a microfluidic system |
US6878271B2 (en) | 2002-09-09 | 2005-04-12 | Cytonome, Inc. | Implementation of microfluidic components in a microfluidic system |
US7455770B2 (en) | 2002-09-09 | 2008-11-25 | Cytonome, Inc. | Implementation of microfluidic components in a microfluidic system |
US6806543B2 (en) | 2002-09-12 | 2004-10-19 | Intel Corporation | Microfluidic apparatus with integrated porous-substrate/sensor for real-time (bio)chemical molecule detection |
JP2006500074A (ja) | 2002-09-27 | 2006-01-05 | オリディス・ビオメド・フォルシュングス−ウント・エントヴィックルングス・ゲーエムベーハー | ポリペプチドおよびこれらをコード化する核酸ならびに肝臓障害および上皮癌の防止、診断、または処置のためのその使用 |
JP2006501449A (ja) * | 2002-09-27 | 2006-01-12 | ザ ジェネラル ホスピタル コーポレーション | 細胞分離のためのマイクロ流体デバイスおよびその使用 |
US7150812B2 (en) * | 2002-10-23 | 2006-12-19 | The Trustees Of Princeton University | Method for continuous particle separation using obstacle arrays asymmetrically aligned to fields |
US6811385B2 (en) | 2002-10-31 | 2004-11-02 | Hewlett-Packard Development Company, L.P. | Acoustic micro-pump |
US7122384B2 (en) | 2002-11-06 | 2006-10-17 | E. I. Du Pont De Nemours And Company | Resonant light scattering microparticle methods |
AU2002952696A0 (en) | 2002-11-14 | 2002-11-28 | Genomics Research Partners Pty Ltd | Status determination |
WO2004051231A1 (ja) * | 2002-11-29 | 2004-06-17 | Nec Corporation | 分離装置および分離方法 |
JP2004354364A (ja) * | 2002-12-02 | 2004-12-16 | Nec Corp | 微粒子操作ユニット、それを搭載したチップと検出装置、ならびにタンパク質の分離、捕獲、および検出方法 |
DE10259703A1 (de) | 2002-12-19 | 2004-07-08 | Ivonex Gmbh | Trennungsverfahren |
US6746503B1 (en) | 2003-01-30 | 2004-06-08 | The Regents Of The University Of California | Precision gap particle separator |
DE602004030220D1 (de) | 2003-02-27 | 2011-01-05 | Veridex Llc | ZIRKULIERENDE TUMORZELLEN (CTC's): FRÜHE BEURTEILUNG VON TIME-TO-PROGRESSION, ÜBERLEBEN UND ANSPRECHEN AUF THERAPIE BEI PATIENTEN MIT METASTASIERTEM KREBS |
DE10313201A1 (de) | 2003-03-21 | 2004-10-07 | Steag Microparts Gmbh | Mikrostrukturierte Trennvorrichtung und mikrofluidisches Verfahren zum Abtrennen von flüssigen Bestandteilen aus einer Flüssigkeit, die Partikel enthält |
US20040197832A1 (en) | 2003-04-03 | 2004-10-07 | Mor Research Applications Ltd. | Non-invasive prenatal genetic diagnosis using transcervical cells |
WO2004101762A2 (en) | 2003-05-12 | 2004-11-25 | The Regents Of The University Of Michigan | Detection and treatment of cancers of the colon |
US6962658B2 (en) | 2003-05-20 | 2005-11-08 | Eksigent Technologies, Llc | Variable flow rate injector |
EP1633481A1 (en) | 2003-06-06 | 2006-03-15 | Micronics, Inc. | System and method for heating, cooling and heat cycling on microfluidic device |
EP1636564A1 (en) * | 2003-06-13 | 2006-03-22 | The General Hospital Corporation | Microfluidic systems for size based removal of red blood cells and platelets from blood |
JP2005037346A (ja) * | 2003-06-25 | 2005-02-10 | Aisin Seiki Co Ltd | マイクロ流体制御システム |
WO2005022147A1 (en) | 2003-08-28 | 2005-03-10 | Celula, Inc. | Methods and apparatus for sorting cells using an optical switch in a microfluidic channel network |
AU2004286201B2 (en) | 2003-09-10 | 2010-09-09 | Altheadx, Inc. | Expression profiling using microarrays |
WO2005028663A2 (en) | 2003-09-18 | 2005-03-31 | Immunivest Corporation | Operator independent programmable sample preparation and analysis system |
EP1692264A2 (en) | 2003-10-28 | 2006-08-23 | The Johns Hopkins University | Quantitative multiplex methylation-specific pcr |
BRPI0415886A (pt) | 2003-10-29 | 2007-01-09 | Mec Dynamics Corp | tiras descartáveis e método de determinação da porcentagem de hbalc na hemoglobina |
JP2007526761A (ja) | 2003-10-31 | 2007-09-20 | ヴィタテックス, インコーポレイテッド | 循環性の腫瘍細胞および内皮細胞の検出のための血液試験のプロトタイプおよび方法 |
WO2005049168A2 (en) | 2003-11-17 | 2005-06-02 | Immunivest Corporation | Method and apparatus for pre-enrichment and recovery of cells from densified whole blood |
US7329391B2 (en) | 2003-12-08 | 2008-02-12 | Applera Corporation | Microfluidic device and material manipulating method using same |
ES2616337T3 (es) | 2003-12-12 | 2017-06-12 | Government Of The United States Of America, As Repr. By The Secr. Of The Dept. Of Health And Human Services And His Successors | Un epítopo de linfocito T citotóxico humano y su epítopo agonista del número no variable de secuencias de repetición en tándem de MUC-1 |
US7939249B2 (en) | 2003-12-24 | 2011-05-10 | 3M Innovative Properties Company | Methods for nucleic acid isolation and kits using a microfluidic device and concentration step |
US20050147977A1 (en) | 2003-12-29 | 2005-07-07 | Tae-Woong Koo | Methods and compositions for nucleic acid detection and sequence analysis |
HUE026132T2 (en) | 2004-01-07 | 2016-05-30 | Novartis Vaccines & Diagnostics Inc | M-CSF-specific monoclonal antibody and its uses |
US7277876B2 (en) * | 2004-01-23 | 2007-10-02 | Solomon Research Llc | Dynamic adaptive distributed computer system |
EP1561507A1 (en) | 2004-01-27 | 2005-08-10 | Future Diagnostics B.V. | System for characterising a fluid, microfluidic device for characterising or analysing concentration components, a method of characterising or analysing such concentrations and a measurement device |
WO2005072399A2 (en) | 2004-01-29 | 2005-08-11 | Massachusetts Institute Of Technology | Microscale sorting cytometer |
US20050181353A1 (en) | 2004-02-17 | 2005-08-18 | Rao Galla C. | Stabilization of cells and biological specimens for analysis |
US20050191636A1 (en) | 2004-03-01 | 2005-09-01 | Biocept, Inc. | Detection of STRP, such as fragile X syndrome |
US20050266433A1 (en) | 2004-03-03 | 2005-12-01 | Ravi Kapur | Magnetic device for isolation of cells and biomolecules in a microfluidic environment |
US20060121624A1 (en) | 2004-03-03 | 2006-06-08 | Huang Lotien R | Methods and systems for fluid delivery |
WO2005085861A2 (en) | 2004-03-03 | 2005-09-15 | Oridis Biomed Forschungs- Und Entwicklungs Gmbh | Nucleic acids and encoded polypeptides for use in liver disorders and epithelial cancer |
EP1765503A2 (en) | 2004-03-03 | 2007-03-28 | The General Hospital Corporation | System for delivering a diluted solution |
CA2559453A1 (en) | 2004-03-12 | 2005-09-29 | The Regents Of The University Of California | Methods and apparatus for integrated cell handling and measurements |
SE0400662D0 (sv) * | 2004-03-24 | 2004-03-24 | Aamic Ab | Assay device and method |
US7390388B2 (en) | 2004-03-25 | 2008-06-24 | Hewlett-Packard Development Company, L.P. | Method of sorting cells on a biodevice |
WO2005098046A2 (en) | 2004-04-01 | 2005-10-20 | Immunivest Corporation | Methods for the determination of cell specific biomarkers |
EP1751306A1 (en) | 2004-04-30 | 2007-02-14 | Yale University | Methods and compositions for cancer diagnosis |
WO2005109238A2 (en) | 2004-05-03 | 2005-11-17 | Cygene Laboratories, Inc. | Method and system for a comprehensive knowledge-based anonymous testing and reporting, and providing selective access to test results and report |
US7468249B2 (en) | 2004-05-05 | 2008-12-23 | Biocept, Inc. | Detection of chromosomal disorders |
WO2005108963A1 (en) | 2004-05-06 | 2005-11-17 | Nanyang Technological University | Microfluidic cell sorter system |
US7858040B2 (en) | 2004-05-07 | 2010-12-28 | Saryna Biotechnologies Llc | Direct mixing and injection for high throughput fluidic systems |
US20050252840A1 (en) | 2004-05-13 | 2005-11-17 | Eksigent Technologies, Llc | Micromixer |
US7622281B2 (en) | 2004-05-20 | 2009-11-24 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and compositions for clonal amplification of nucleic acid |
WO2005116264A2 (en) | 2004-05-24 | 2005-12-08 | Immunivest Corporation | A blood test to monitor the genetic changes of progressive cancer using immunomagnetic enrichment and fluorescence in situ hybridization (fish) |
EP1607485A1 (en) | 2004-06-14 | 2005-12-21 | Institut National De La Sante Et De La Recherche Medicale (Inserm) | Method for quantifying VEGF121 isoform in a biological sample |
DE102004036669A1 (de) | 2004-07-28 | 2006-03-23 | Otto Bock Healthcare Gmbh | Pumpe mit einem mit wenigstens einer flexiblen Wandung abgeschlossenen Fluidvolumen |
US20060051265A1 (en) | 2004-09-08 | 2006-03-09 | Health Research, Inc. | Apparatus and method for sorting microstructures in a fluid medium |
WO2006035846A1 (ja) | 2004-09-30 | 2006-04-06 | Dainippon Ink And Chemicals, Inc. | 多孔質金属焼結体の製造方法 |
DE102004047953A1 (de) | 2004-10-01 | 2006-04-20 | Rudolf Rigler | Selektion von Partikeln im laminaren Fluss |
WO2006076567A2 (en) | 2005-01-13 | 2006-07-20 | Micronics, Inc. | Microfluidic rare cell detection device |
US8158410B2 (en) * | 2005-01-18 | 2012-04-17 | Biocept, Inc. | Recovery of rare cells using a microchannel apparatus with patterned posts |
EP1838442B1 (en) | 2005-01-18 | 2013-09-11 | Biocept, Inc. | Cell separation using microchannel having patterned posts |
US20060252087A1 (en) | 2005-01-18 | 2006-11-09 | Biocept, Inc. | Recovery of rare cells using a microchannel apparatus with patterned posts |
US7981696B2 (en) | 2005-02-18 | 2011-07-19 | The United States of America, as represented by the Secretary of Commerce, The National Institute of Standards and Technology | Microfluidic platform of arrayed switchable spin-valve elements for high-throughput sorting and manipulation of magnetic particles and biomolecules |
US20070026418A1 (en) | 2005-07-29 | 2007-02-01 | Martin Fuchs | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
US20070196820A1 (en) | 2005-04-05 | 2007-08-23 | Ravi Kapur | Devices and methods for enrichment and alteration of cells and other particles |
US20070026413A1 (en) | 2005-07-29 | 2007-02-01 | Mehmet Toner | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
US20060223178A1 (en) | 2005-04-05 | 2006-10-05 | Tom Barber | Devices and methods for magnetic enrichment of cells and other particles |
US20070026415A1 (en) | 2005-07-29 | 2007-02-01 | Martin Fuchs | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
US20070026417A1 (en) | 2005-07-29 | 2007-02-01 | Martin Fuchs | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
EP2594631A1 (en) | 2005-04-05 | 2013-05-22 | Cellpoint Diagnostics | Devices and method for detecting circulating tumor cells and other particles |
US20070026414A1 (en) | 2005-07-29 | 2007-02-01 | Martin Fuchs | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
EP1886138A4 (en) * | 2005-05-11 | 2009-04-15 | Genetic Technologies Ltd | PROCESS FOR ENRICHING FETTAL CELLS |
US20070059680A1 (en) | 2005-09-15 | 2007-03-15 | Ravi Kapur | System for cell enrichment |
US8921102B2 (en) | 2005-07-29 | 2014-12-30 | Gpb Scientific, Llc | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
US20070026419A1 (en) | 2005-07-29 | 2007-02-01 | Martin Fuchs | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
US20070026416A1 (en) | 2005-07-29 | 2007-02-01 | Martin Fuchs | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
US20070059683A1 (en) | 2005-09-15 | 2007-03-15 | Tom Barber | Veterinary diagnostic system |
US20070059719A1 (en) | 2005-09-15 | 2007-03-15 | Michael Grisham | Business methods for prenatal Diagnosis |
US20070059718A1 (en) | 2005-09-15 | 2007-03-15 | Mehmet Toner | Systems and methods for enrichment of analytes |
US20070059781A1 (en) | 2005-09-15 | 2007-03-15 | Ravi Kapur | System for size based separation and analysis |
US20070059774A1 (en) | 2005-09-15 | 2007-03-15 | Michael Grisham | Kits for Prenatal Testing |
JP2009509143A (ja) | 2005-09-15 | 2009-03-05 | アルテミス ヘルス,インク. | 分析改善システムおよび方法 |
US20070059716A1 (en) | 2005-09-15 | 2007-03-15 | Ulysses Balis | Methods for detecting fetal abnormality |
EP1943354A2 (en) * | 2005-10-21 | 2008-07-16 | Monaliza Medical Ltd. | Methods and kits for analyzing genetic material of a fetus |
PT2385143T (pt) * | 2006-02-02 | 2016-10-18 | Univ Leland Stanford Junior | Rastreio genético fetal não-invasivo por análise digital |
WO2007112418A2 (en) * | 2006-03-28 | 2007-10-04 | Baylor College Of Medicine | Screening for down syndrome |
US7735652B2 (en) * | 2006-06-01 | 2010-06-15 | The Trustees Of Princeton University | Apparatus and method for continuous particle separation |
-
2005
- 2005-09-15 US US11/227,904 patent/US20070196820A1/en not_active Abandoned
-
2006
- 2006-04-05 JP JP2008505514A patent/JP2008538283A/ja not_active Withdrawn
- 2006-04-05 AU AU2006232103A patent/AU2006232103A1/en not_active Abandoned
- 2006-04-05 EP EP20120169261 patent/EP2492339B1/en active Active
- 2006-04-05 CN CN2006800198868A patent/CN101918527B/zh active Active
- 2006-04-05 CA CA 2601480 patent/CA2601480A1/en not_active Abandoned
- 2006-04-05 EP EP06740612A patent/EP1866410B1/en active Active
- 2006-04-05 WO PCT/US2006/012820 patent/WO2006108101A2/en active Application Filing
- 2006-04-05 EP EP13156633.3A patent/EP2664666B1/en active Active
- 2006-04-05 GB GB0612648A patent/GB2429774A/en not_active Withdrawn
- 2006-06-08 US US11/449,149 patent/US8021614B2/en active Active
-
2011
- 2011-09-14 US US13/232,781 patent/US9174222B2/en active Active
-
2012
- 2012-04-20 US US13/452,054 patent/US8585971B2/en active Active
-
2013
- 2013-02-26 HK HK13102405A patent/HK1175494A1/zh unknown
-
2015
- 2015-11-02 US US14/930,313 patent/US9956562B2/en active Active
-
2018
- 2018-04-27 US US15/965,128 patent/US10786817B2/en active Active
-
2020
- 2020-09-15 US US17/021,100 patent/US20210178403A1/en active Pending
Cited By (26)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110186835A (zh) * | 2013-03-15 | 2019-08-30 | Gpb科学有限责任公司 | 颗粒的片上微流体处理 |
CN110186835B (zh) * | 2013-03-15 | 2022-05-31 | Gpb科学有限公司 | 颗粒的片上微流体处理 |
CN105431537A (zh) * | 2013-08-07 | 2016-03-23 | 罗伯特·博世有限公司 | 用于处理包含靶细胞和伴随细胞的生物材料样品以提取靶细胞的核酸的方法和装置 |
CN105473716A (zh) * | 2013-08-07 | 2016-04-06 | 罗伯特·博世有限公司 | 用于处理包含靶细胞和伴随细胞的生物材料样品以提取靶细胞的核酸的方法和装置 |
US10072258B2 (en) | 2013-08-07 | 2018-09-11 | Robert Bosch Gmbh | Method and device for preparing a sample of biological material containing target cells and accompanying cells for extracting nucleic acids of the target cells |
CN104212708A (zh) * | 2014-09-19 | 2014-12-17 | 张蓉 | 一种肠道内容物目标成分提取装置 |
CN107429262A (zh) * | 2015-01-07 | 2017-12-01 | 英迪公司 | 一种用于机械和水动力微流体转染的方法以及用于其的设备 |
CN107206380A (zh) * | 2015-01-23 | 2017-09-26 | 和卓生物科技(上海)有限公司 | 用于非侵入性产前测试的基于微流体的胎儿细胞的检测和分离 |
CN114272966B (zh) * | 2015-07-22 | 2023-11-07 | 北卡罗来纳-查佩尔山大学 | 含有具有空间分离的珠保留和信号检测段的珠孔几何结构的流体装置和相关方法 |
CN114272966A (zh) * | 2015-07-22 | 2022-04-05 | 北卡罗来纳-查佩尔山大学 | 含有具有空间分离的珠保留和信号检测段的珠孔几何结构的流体装置和相关方法 |
CN108495922B (zh) * | 2016-01-22 | 2022-10-28 | 小利兰·斯坦福大学托管委员会 | 一种用于从未处理的精液中选择性分选高活动和形态正常的精子的微流体装置 |
CN108495922A (zh) * | 2016-01-22 | 2018-09-04 | 小利兰·斯坦福大学托管委员会 | 一种用于从未处理的精液中选择性分选高活动和形态正常的精子的微流体装置 |
CN109311023B (zh) * | 2016-04-22 | 2021-11-26 | 普渡研究基金会 | 高通量颗粒捕获与分析 |
CN109311023A (zh) * | 2016-04-22 | 2019-02-05 | 普渡研究基金会 | 高通量颗粒捕获与分析 |
CN106119189A (zh) * | 2016-07-11 | 2016-11-16 | 山东亚大药业有限公司 | 胎儿有核红细胞的分离纯化方法及试剂盒 |
CN109562380A (zh) * | 2016-08-02 | 2019-04-02 | Imec 非营利协会 | 用于聚集流中的物体的方法和装置 |
CN110799840A (zh) * | 2016-10-23 | 2020-02-14 | 伯克利之光生命科技公司 | 筛选b细胞淋巴细胞的方法 |
CN110799840B (zh) * | 2016-10-23 | 2022-03-01 | 伯克利之光生命科技公司 | 筛选b细胞淋巴细胞的方法 |
CN111212900A (zh) * | 2017-10-03 | 2020-05-29 | Nok株式会社 | 细胞捕获装置 |
CN111212900B (zh) * | 2017-10-03 | 2023-09-29 | Nok株式会社 | 细胞捕获装置 |
CN107723207B (zh) * | 2017-11-01 | 2019-01-01 | 深圳市瑞格生物科技有限公司 | 一种分离捕获细胞的芯片及其在肿瘤细胞分选中的应用 |
CN107723207A (zh) * | 2017-11-01 | 2018-02-23 | 深圳市瑞格生物科技有限公司 | 一种分离捕获细胞的芯片及其在肿瘤细胞分选中的应用 |
CN110885742A (zh) * | 2018-05-25 | 2020-03-17 | 昆山汇先医药技术有限公司 | 富集筛选机构及其制备方法 |
CN110885742B (zh) * | 2018-05-25 | 2023-11-10 | 江苏汇先医药技术有限公司 | 富集筛选机构及其制备方法 |
CN112912725A (zh) * | 2018-10-25 | 2021-06-04 | Tl基因体科技株式会社 | 使用微通道对血液进行预处理以对血细胞进行分级 |
CN110862905A (zh) * | 2020-01-09 | 2020-03-06 | 北京航空航天大学合肥创新研究院 | 用于细胞迁移实验的芯片装置、制备方法及实验方法 |
Also Published As
Publication number | Publication date |
---|---|
US20160144378A1 (en) | 2016-05-26 |
WO2006108101A3 (en) | 2009-04-16 |
GB2429774A (en) | 2007-03-07 |
GB0612648D0 (en) | 2006-08-16 |
US10786817B2 (en) | 2020-09-29 |
CA2601480A1 (en) | 2006-10-12 |
EP1866410B1 (en) | 2012-08-08 |
EP1866410A2 (en) | 2007-12-19 |
JP2008538283A (ja) | 2008-10-23 |
EP2664666A2 (en) | 2013-11-20 |
AU2006232103A1 (en) | 2006-10-12 |
EP2492339A1 (en) | 2012-08-29 |
US8021614B2 (en) | 2011-09-20 |
EP1866410A4 (en) | 2009-11-04 |
US20210178403A1 (en) | 2021-06-17 |
EP2492339B1 (en) | 2014-03-19 |
US20120225473A1 (en) | 2012-09-06 |
WO2006108101A2 (en) | 2006-10-12 |
US20190001344A1 (en) | 2019-01-03 |
US8585971B2 (en) | 2013-11-19 |
EP2664666B1 (en) | 2017-06-07 |
US9174222B2 (en) | 2015-11-03 |
US20070026381A1 (en) | 2007-02-01 |
HK1175494A1 (zh) | 2013-07-05 |
US20120196273A1 (en) | 2012-08-02 |
US20070196820A1 (en) | 2007-08-23 |
EP2664666A3 (en) | 2014-03-12 |
CN101918527B (zh) | 2012-06-27 |
US9956562B2 (en) | 2018-05-01 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN101918527B (zh) | 富集和改变细胞和其他微粒的装置和方法 | |
US10500526B1 (en) | Method and device for high-throughput solution exchange for cell and particle suspensions | |
US8986966B2 (en) | Microfluidic device for cell separation and uses thereof | |
CN101305087A (zh) | 用于磁富集细胞和其他颗粒的装置和方法 | |
Talebjedi et al. | Exploiting microfluidics for extracellular vesicle isolation and characterization: potential use for standardized embryo quality assessment | |
CA2622779A1 (en) | Devices and methods for magnetic enrichment of cells and other particles | |
US10226769B2 (en) | Method and device for high-throughput solution exchange for cell and particle suspensions | |
RU2423698C1 (ru) | Способ селективного выделения популяции жизнеспособных клеток из биологических жидкостей | |
Zhang et al. | On-chip sample preparations for point-of-care cellular analysis of blood |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
ASS | Succession or assignment of patent right |
Owner name: GPB TECHNOLOGICA,JANVES ATEL DES Effective date: 20121112 Owner name: INTEGRATED HOSPITAL COMPANY Free format text: FORMER OWNER: VERINATA HEALTH, INC. Effective date: 20121112 Free format text: FORMER OWNER: INTEGRATED HOSPITAL COMPANY Effective date: 20121112 |
|
C41 | Transfer of patent application or patent right or utility model | ||
C56 | Change in the name or address of the patentee |
Owner name: VERINATA HEALTH, INC. Free format text: FORMER NAME: ARTEMIS HEALTHCARE SERVICES LLC |
|
CP03 | Change of name, title or address |
Address after: California, USA Patentee after: VERINATA HEALTH, Inc. Patentee after: THE GENERAL Hospital Corp. Address before: Massachusetts, USA Patentee before: Artemis Healthcare Patentee before: The General Hospital Corp. |
|
TR01 | Transfer of patent right |
Effective date of registration: 20121112 Address after: Massachusetts, USA Patentee after: THE GENERAL Hospital Corp. Patentee after: GPB Technology Ltd. Address before: Massachusetts, USA Patentee before: The General Hospital Corp. Effective date of registration: 20121112 Address after: Massachusetts, USA Patentee after: THE GENERAL Hospital Corp. Address before: California, USA Patentee before: VERINATA HEALTH, Inc. Patentee before: The General Hospital Corp. |